2,196 results match your criteria glucosinolate metabolism


Cytosolic phosphofructokinases are important for sugar homeostasis in leaves of Arabidopsis thaliana.

Ann Bot 2021 Sep 22. Epub 2021 Sep 22.

Plant Physiology, University Bayreuth, Universitaetsstr. 30, 95440 Bayreuth, Germany.

Background And Aims: ATP-dependent phosphofructokinases (PFKs) catalyse phosphorylation of the carbon-1 position of fructose-6-phosphate, to form fructose-1,6-bisphosphate. In the cytosol, this is considered a key step in channelling carbon into glycolysis. Arabidopsis thaliana has seven genes encoding PFK isoforms, two chloroplastic and five cytosolic. Read More

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September 2021

Effects of on Plant Development, Glucosinolate Metabolism, and Resistance in Transgenic .

Front Plant Sci 2021 3;12:707054. Epub 2021 Sep 3.

Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou, China.

MYC2/3/4, known as a basic helix-loop-helix (bHLH) transcription factor, directly activate the genes involved in diverse plant development and secondary metabolites biosynthesis. In this study, we identified and cloned five paralogs (////) from Chinese cabbage ( ssp. ). Read More

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September 2021

Transcriptomic Reprograming of pv. after Treatment with Hydrolytic Products Derived from Glucosinolates.

Plants (Basel) 2021 Aug 11;10(8). Epub 2021 Aug 11.

Group of Genetics, Breeding and Biochemistry of Brassicas, Misión Biológica de Galicia, Spanish Council for Scientific Research (MBG-CSIC), 36143 Pontevedra, Spain.

The bacterium pv. () causes black rot disease in crops. Glucosinolates are known to be part of the defence system of crops against infection. Read More

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The Metabolism of Glucosinolates by Gut Microbiota.

Nutrients 2021 Aug 10;13(8). Epub 2021 Aug 10.

Department of the Sciences of Agriculture, Food, Natural Resources, and Engineering (DAFNE) the University of Foggia, Via Napoli 25, 71122 Foggia, Italy.

Glucosinolates (GLS) and their derivatives are secondary plant metabolites abundant in . Due to the enzymatic reaction between GLS and myrosinase enzyme, characteristic compounds with a pungent taste are formed, used by plants to defend themselves against insect herbivores. These GLS derivatives have an important impact on human health, including anti-inflammation and anti-cancer effects. Read More

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The Inhibitory Effect of Sulforaphane on Bladder Cancer Cell Depends on GSH Depletion-Induced by Nrf2 Translocation.

Molecules 2021 Aug 13;26(16). Epub 2021 Aug 13.

Norwich Medical School, University of East Anglia, Norwich NR4 7UQ, UK.

Sulforaphane (SFN), an isothiocyanate (ITCs) derived from glucosinolate that is found in cruciferous vegetables, has been reported to exert a promising anticancer effect in a substantial amount of scientific research. However, epidemical studies showed inconsistencies between cruciferous vegetable intake and bladder cancer risk. In this study, human bladder cancer T24 cells were used as in vitro model for revealing the inhibitory effect and its potential mechanism of SFN on cell growth. Read More

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Sprouts Grown under Elevated CO Hyperaccumulate Glucosinolates and Antioxidants and Exhibit Enhanced Biological and Reduced Antinutritional Properties.

Biomolecules 2021 08 9;11(8). Epub 2021 Aug 9.

Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza 12613, Egypt.

The nutritional and health-promoting properties of plants are largely determined by their tissue chemistry. Tuning growth conditions could affect the accumulation of phytochemicals and, therefore, enhance the biological activities. Herein, the impact of elevated CO (eCO; 620 µmol CO mol air) on growth and chemical composition of sprouts of three cultivars (Haraz, Khider and Rajab) was investigated. Read More

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Potential glucosinolate genes identified from the co-expression modules using graph clustering approach.

PeerJ 2021 4;9:e11876. Epub 2021 Aug 4.

Centre for Bioinformatics Research, Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, UKM Bangi, Selangor, Malaysia.

Background: Glucosinolates (GSLs) are plant secondary metabolites that contain nitrogen-containing compounds. They are important in the plant defense system and known to provide protection against cancer in humans. Currently, increasing the amount of data generated from various omics technologies serves as a hotspot for new gene discovery. Read More

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The phosphorylated pathway of serine biosynthesis is crucial for indolic glucosinolate biosynthesis and plant growth promotion conferred by the root endophyte Colletotrichum tofieldiae.

Plant Mol Biol 2021 Sep 23;107(1-2):85-100. Epub 2021 Aug 23.

Institute for Plant Sciences, Biocenter University of Cologne, Zülpicher Straße 47b, 50674, Cologne, Germany.

Key Message: Phosphoglycerate Dehydrogenase 1 of the phosphorylated pathway of serine biosynthesis, active in heterotrophic plastids, is required for the synthesis of serine to enable plant growth at high rates of indolic glucosinolate biosynthesis. Plants have evolved effective strategies to defend against various types of pathogens. The synthesis of a multitude of specialized metabolites represents one effective approach to keep plant attackers in check. Read More

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September 2021

Comparative transcriptomic analyses of glucosinolate metabolic genes during the formation of Chinese kale seeds.

BMC Plant Biol 2021 Aug 21;21(1):394. Epub 2021 Aug 21.

College of Horticulture, Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Background: To understand the mechanism of glucosinolates (GSs) accumulation in the specific organs, combined analysis of physiological change and transcriptome sequencing were applied in the current study. Taking Chinese kale as material, seeds and silique walls were divided into different stages based on the development of the embryo in seeds and then subjected to GS analysis and transcriptome sequencing.

Results: The main GS in seeds of Chinese kale were glucoiberin and gluconapin and their content changed with the development of the seed. Read More

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Exploration of the Characteristics of Intestinal Microbiota and Metabolomics in Different Rat Models of Mongolian Medicine.

Evid Based Complement Alternat Med 2021 3;2021:5532069. Epub 2021 Aug 3.

Inner Mongolia University for Nationalities, Mongolian Medical College, Tongliao City, Inner Mongolia Autonomous Region 028000, China.

Background: Mongolian medicine is a systematic theoretical system, which is based on the balance among Heyi, Xila, and Badagan. However, the underlying mechanisms remain unclear. This study aimed to explore the characteristics of intestinal microbiota and metabolites in different rat models of Mongolian medicine. Read More

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Extra-large G-proteins influence plant response to Sclerotinia sclerotiorum by regulating glucosinolate metabolism in Brassica juncea.

Mol Plant Pathol 2021 Oct 10;22(10):1180-1194. Epub 2021 Aug 10.

National Institute of Plant Genome Research, New Delhi, India.

Heterotrimeric G-proteins are one of the highly conserved signal transducers across phyla. Despite the obvious importance of G-proteins in controlling various plant growth and environmental responses, there is no information describing the regulatory complexity of G-protein networks during pathogen response in a polyploid crop. Here, we investigated the role of extra-large G-proteins (XLGs) in the oilseed crop Brassica juncea, which has inherent susceptibility to the necrotrophic fungal pathogen Sclerotinia sclerotiorum. Read More

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October 2021

Precursor-Boosted Production of Metabolites in Microshoots Grown in Plantform Bioreactors, and Antioxidant and Antimicrobial Activities of Biomass Extracts.

Molecules 2021 Jul 31;26(15). Epub 2021 Jul 31.

Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, Poland.

The study demonstrated the effects of precursor feeding on the production of glucosinolates (GSLs), flavonoids, polyphenols, saccharides, and photosynthetic pigments in microshoot cultures grown in Plantform bioreactors. It also evaluated the antioxidant and antimicrobial activities of extracts. L-phenylalanine (Phe) and L-tryptophan (Trp) as precursors were tested at 0. Read More

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Combination of Selenium and UVA Radiation Affects Growth and Phytochemicals of Broccoli Microgreens.

Molecules 2021 Jul 31;26(15). Epub 2021 Jul 31.

College of Horticulture, South China Agricultural University, Guangzhou 510642, China.

Addition of selenium or application of ultraviolet A (UVA) radiation for crop production could be an effective way of producing phytochemical-rich food. This study was conducted to investigate the effects of selenium and UVA radiation, as well as their combination on growth and phytochemical contents in broccoli microgreens. There were three treatments: Se (100 μmol/L NaSeO), UVA (40 μmol/m/s) and Se + UVA (with application of Se and UVA). Read More

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Sulforaphane as a potential remedy against cancer: Comprehensive mechanistic review.

J Food Biochem 2021 Aug 5:e13886. Epub 2021 Aug 5.

Department of Food Science and Technology, Minhaj University, Lahore, Pakistan.

Sulforaphane belongs to the active class of isothiocyanates capable of delivering various biological benefits for health promotion and disease prevention. This compound is considered vital to curtail numerous metabolic disorders. Various studies have proven its beneficial effects against cancer prevention and its possible utilization as a therapeutic agent in cancer treatment. Read More

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Practical Approach To Explore the Effects of Polyphenols on Aryl Hydrocarbon Receptor Regulated Immune Function.

J Agric Food Chem 2021 Aug 2;69(31):8625-8633. Epub 2021 Aug 2.

Malaghan Institute of Medical Research, Post Office Box 7060, Wellington 6242, New Zealand.

The ligand-activated aryl hydrocarbon receptor (AhR) is an important molecular regulator of immune function, whose activity can be modulated by dietary glucosinolate- and tryptophan-derived metabolites. In contrast, the potential use of polyphenols as dietary regulators of AhR-dependent immunity remains unclear. In this perspective, we discuss how cellular metabolism may alter the net effect of polyphenols on AhR, thus potentially reconciling some of the conflicting observations reported in the literature. Read More

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Metabolite Profiling and Transcriptome Analysis Provide Insight into Seed Coat Color in .

Int J Mol Sci 2021 Jul 5;22(13). Epub 2021 Jul 5.

Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China.

The allotetraploid species (mustard) is grown worldwide as oilseed and vegetable crops; the yellow seed-color trait is particularly important for oilseed crops. Here, to examine the factors affecting seed coat color, we performed a metabolic and transcriptomic analysis of yellow- and dark-seeded . seeds. Read More

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The Arabidopsis Iron-Sulfur (Fe-S) Cluster Gene Plays a Role in Host and Nonhost Disease Resistance by Accumulation of Defense-Related Metabolites.

Int J Mol Sci 2021 Jul 1;22(13). Epub 2021 Jul 1.

Noble Research Institute, Ardmore, OK 73401, USA.

Until recently, genes from the iron-sulfur (Fe-S) cluster pathway were not known to have a role in plant disease resistance. The () and () genes are part of a set of 27 Fe-S cluster genes induced after infection with host and nonhost pathogens in Arabidopsis. A role for in plant immunity was recently demonstrated. Read More

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Changes in glucosinolates and their breakdown products during the fermentation of cabbage and prolonged storage of sauerkraut: Focus on sauerkraut juice.

Food Chem 2021 Dec 30;365:130498. Epub 2021 Jun 30.

Department of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10 Str., 10-748 Olsztyn, Poland. Electronic address:

Sauerkraut juice has not gained much scientific attention to date. Therefore, this study aimed to track changes in glucosinolates (GLS) during fermentation of white cabbage and the formation of corresponding breakdown products in sauerkraut and sauerkraut juice separately and to evaluate their stability during prolonged storage of the final products. The results obtained indicate that both sauerkraut and sauerkraut juice are a good source of bioactive compounds: ascorbigen and isothiocyanates. Read More

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December 2021

Validity of dietary isothiocyanate intake estimates from a food frequency questionnaire using 24 h urinary isothiocyanate excretion as an objective biomarker: the JPHC-NEXT protocol area.

Eur J Clin Nutr 2021 Jul 6. Epub 2021 Jul 6.

Epidemiology and Prevention Group, Center for Public Health Sciences, National Cancer Center, Tokyo, Japan.

Background/objectives: Isothiocyanate (ITC) is formed via the hydrolysis of glucosinolates by myrosinase, found in cruciferous vegetables. Although myrosinase is inactivated by the cooking process, no studies have incorporated the effect of cooking into the estimation of dietary ITC intake or evaluated the validity. We evaluated the validity of dietary ITC intake estimated from a food frequency questionnaire (FFQ), and urinary ITC levels using 24 h urine samples or a WFR (weighed food record), and evaluated the reproducibility of dietary ITC in two FFQs administered at an interval of 1-year. Read More

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Effects of the Developmental Regulator BOLITA on the Plant Metabolome.

Genes (Basel) 2021 Jun 29;12(7). Epub 2021 Jun 29.

Cell Identity Laboratory, Biotechnology and Biochemistry Department, CINVESTAV-IPN Irapuato Unit, Irapuato 36824, Mexico.

Transcription factors are important regulators of gene expression. They can orchestrate the activation or repression of hundreds or thousands of genes and control diverse processes in a coordinated way. This work explores the effect of a master regulator of plant development, BOLITA (BOL), in plant metabolism, with a special focus on specialized metabolism. Read More

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Overexpression Confers Heat Shock Tolerance in Transgenic through Inherent Chaperone Activity, Induced Glucosinolate Biosynthesis, and Differential Regulation of Abiotic Stress Response Genes.

Int J Mol Sci 2021 Jun 16;22(12). Epub 2021 Jun 16.

Department of Agricultural Biotechnology, National Institute of Agricultural Sciences (NAS), Rural Development Administration, Jeonju 54874, Korea.

Plant phosphoprotein phosphatases are ubiquitous and multifarious enzymes that respond to developmental requirements and stress signals through reversible dephosphorylation of target proteins. In this study, we investigated the hitherto unknown functions of protein phosphatase 5.2 () by transgenic overexpression of lines. Read More

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Disentangling transcriptional responses in plant defense against arthropod herbivores.

Sci Rep 2021 06 21;11(1):12996. Epub 2021 Jun 21.

Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.

The success in the response of a plant to a pest depends on the regulatory networks that connect plant perception and plant response. Meta-analyses of transcriptomic responses are valuable tools to discover novel mechanisms in the plant/herbivore interplay. Considering the quantity and quality of available transcriptomic analyses, Arabidopsis thaliana was selected to test the ability of comprehensive meta-analyses to disentangle plant responses. Read More

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CRISPR-Cas9-Mediated Gene Editing of Genes Impair Glucoraphanin Accumulation of in the Field.

CRISPR J 2021 06;4(3):416-426

John Innes Centre, Norwich Research Park, Norwich, United Kingdom and Norwich Research Park, Norwich, United Kingdom.

Discoveries in model plants grown under optimal conditions can provide important directions for crop improvement. However, it is important to verify whether results can be translated to crop plants grown in the field. In this study, we sought to study the role of in the regulation of aliphatic glucosinolate (A-GSL) biosynthesis and associated sulfur metabolism in field-grown with the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 gene-editing technology. Read More

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Maca extracts regulate glucose and lipid metabolism in insulin-resistant HepG2 cells via the PI3K/AKT signalling pathway.

Food Sci Nutr 2021 Jun 29;9(6):2894-2907. Epub 2021 Mar 29.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology Jiangnan University Wuxi China.

This work focused on the separation of the active ingredients of maca ( Walpers) and evaluated the antioxidative capability of these components with effects on improving glucose and lipid metabolism in insulin-resistant HepG2 cells. DPPH free radical scavenging and reducing power assays were used to evaluate the antioxidant activity of maca extracts. An insulin-resistant HepG2 cell model induced by glucose, fructose, oleic acid, and palmitic acid was adopted to investigate the effects of maca extracts on regulating glucose and lipid metabolism in this study. Read More

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Effects of selenate on Se, flavonoid, and glucosinolate in broccoli florets by combined transcriptome and metabolome analyses.

Food Res Int 2021 08 31;146:110463. Epub 2021 May 31.

College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China. Electronic address:

Broccoli is a nutritious vegetable popular all over the world. This study investigated the effects of different concentrations of selenate (0, 0.1, 0. Read More

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Proteomic analysis validates previous findings on wounding-responsive plant hormone signaling and primary metabolism contributing to the biosynthesis of secondary metabolites based on metabolomic analysis in harvested broccoli (Brassica oleracea L. var. italica).

Food Res Int 2021 07 5;145:110388. Epub 2021 May 5.

College of Life Science, Dalian Minzu University, Dalian 116600, China; Key Laboratory of Biotechnology and Bioesources Utilization, Ministry of Education, Dalian 116600, China.

The plant wound-response is a complex process that generates physiological modifications for protecting the wounded tissue. In this study, tandem mass tag (TMT)-based quantitative proteomic analysis was performed to clarify the comprehensive molecular mechanism for the wound-response of broccoli subjected to two wounding intensities (0.04 and 1. Read More

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Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms.

Biotechnol Adv 2021 Jun 6:107784. Epub 2021 Jun 6.

Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Chemistry Department, School of Sciences & Engineering, The American University in Cairo, New Cairo, Egypt. Electronic address:

Glucosinolate-producing plants have long been recognized for both their distinctive benefits to human nutrition and their resistance traits against pathogens and herbivores. Despite the accumulation of glucosinolates (GLS) in plants is associated with their resistance to various biotic and abiotic stresses, the defensive and biological activities of GLS are commonly conveyed by their metabolic products. In view of this, metabolism is considered the driving factor upon the interactions of GLS-producing plants with other organisms, also influenced by plant and plant attacking or digesting organism characteristics. Read More

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Transcriptome and metabolome profiling to elucidate mechanisms underlying the blue discoloration of radish roots during storage.

Food Chem 2021 Nov 11;362:130076. Epub 2021 May 11.

Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing 100097, China. Electronic address:

The internal blue discoloration of radish roots (Raphanus sativus) during storage affects their quality. We here performed transcriptome and metabolome profiling to investigate the mechanisms underlying the bluing of radish roots during storage. On comparing white radish (WR) and blue radish (BR), we identified 14,171 differentially expressed genes (upregulated: 7,383, downregulated: 6,788) and 145 differentially accumulated metabolites (upregulated: 117, downregulated: 28). Read More

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November 2021

Carbon monoxide exposure activates ULK1 via AMPK phosphorylation in murine embryonic fibroblasts.

Int J Vitam Nutr Res 2021 Jun 2:1-10. Epub 2021 Jun 2.

Institute of Biochemistry and Molecular Biology I, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.

Carbon monoxide (CO) is endogenously produced upon degradation of heme by heme oxygenases (HOs) and is suggested to act as a gaseous signaling molecule. The expression of HO-1 is triggered by the Nrf2-Keap1 signaling pathway which responds to exogenous stress signals and dietary constituents such as flavonoids and glucosinolates or reactive metabolic intermediates like 4-hydroxynonenal. Endogenous CO affects energy metabolism, regulates the utilization of glucose and addresses CYP450 enzymes. Read More

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Sulforaphane Impact on Reactive Oxygen Species (ROS) in Bladder Carcinoma.

Int J Mol Sci 2021 May 31;22(11). Epub 2021 May 31.

Department of Urology, Goethe-University, 60590 Frankfurt am Main, Germany.

Sulforaphane (SFN) is a natural glucosinolate found in cruciferous vegetables that acts as a chemopreventive agent, but its mechanism of action is not clear. Due to antioxidative mechanisms being thought central in preventing cancer progression, SFN could play a role in oxidative processes. Since redox imbalance with increased levels of reactive oxygen species (ROS) is involved in the initiation and progression of bladder cancer, this mechanism might be involved when chemoresistance occurs. Read More

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