42,411 results match your criteria glucose uptake

Tumor-derived exosomes drive immunosuppressive macrophages in a pre-metastatic niche through glycolytic dominant metabolic reprogramming.

Cell Metab 2021 Sep 17. Epub 2021 Sep 17.

Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, KY, USA; Division of Immunotherapy, The Hiram C. Polk, Jr., MD Department of Surgery, Immuno-Oncology Program, Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA. Electronic address:

One of the defining characteristics of a pre-metastatic niche, a fundamental requirement for primary tumor metastasis, is infiltration of immunosuppressive macrophages. How these macrophages acquire their phenotype remains largely unexplored. Here, we demonstrate that tumor-derived exosomes (TDEs) polarize macrophages toward an immunosuppressive phenotype characterized by increased PD-L1 expression through NF-kB-dependent, glycolytic-dominant metabolic reprogramming. Read More

View Article and Full-Text PDF
September 2021

Uteroplacental nutrient flux and evidence for metabolic reprogramming during sustained hypoxemia.

Physiol Rep 2021 Sep;9(18):e15033

Perinatal Research Center, Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado, USA.

Gestational hypoxemia is often associated with reduced birth weight, yet how hypoxemia controls uteroplacental nutrient metabolism and supply to the fetus is unclear. This study tested the effects of maternal hypoxemia (HOX) between 0.8 and 0. Read More

View Article and Full-Text PDF
September 2021

Role of mass spectrometry in the study of interactions between amylin and metal ions.

Mass Spectrom Rev 2021 Sep 24. Epub 2021 Sep 24.

Department of Medicine, University of Padova, Padova, Italy.

Amylin (islet amyloid polypeptide [IAPP]) is a neuroendocrine hormone synthesized with insulin in the beta cells of pancreatic islets. The two hormones act in different ways: in fact insulin triggers glucose uptake in muscle and liver cells, removing glucose from the bloodstream and making it available for energy use and storage, while amylin regulates glucose homeostasis. Aside these positive physiological aspects, human amyloid polypeptide (hIAPP) readily forms amyloid in vitro. Read More

View Article and Full-Text PDF
September 2021

Combination of Metformin and Exercise in Management of Metabolic Abnormalities Observed in Type 2 Diabetes Mellitus.

Filip Jevtovic

Diabetes Metab Syndr Obes 2021 16;14:4043-4057. Epub 2021 Sep 16.

Department of Kinesiology, College of Health and Human Performance, East Carolina University, Greenville, NC, USA.

Excess nutrient intake and lack of exercise characterize the problem of obesity and are common factors in insulin resistance (IR). With an increasing number of prediabetic, and type 2 diabetic populations, metformin is still the most prescribed glucose-lowering drug and is often accompanied by recommendations for regular physical exercise. Metformin, by the inhibition of complex 1 of the electron transport chain, and exercise, by increasing energy expenditure, both elicit a low cellular energy state that leads to improvements in glucose control via activation of adenosine 5' monophosphate-activated protein kinase (AMPK). Read More

View Article and Full-Text PDF
September 2021

Molecular mechanism of sugar transport in plants unveiled by structures of glucose/H symporter STP10.

Nat Plants 2021 Sep 23. Epub 2021 Sep 23.

Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.

Sugars are essential sources of energy and carbon and also function as key signalling molecules in plants. Sugar transport proteins (STP) are proton-coupled symporters responsible for uptake of glucose from the apoplast into plant cells. They are integral to organ development in symplastically isolated tissues such as seed, pollen and fruit. Read More

View Article and Full-Text PDF
September 2021

Targeting MYC-enhanced glycolysis for the treatment of small cell lung cancer.

Cancer Metab 2021 Sep 23;9(1):33. Epub 2021 Sep 23.

Department of Thoracic/Head and Neck Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Introduction: The transcription factor MYC is overexpressed in 30% of small cell lung cancer (SCLC) tumors and is known to modulate the balance between two major pathways of metabolism: glycolysis and mitochondrial respiration. This duality of MYC underscores the importance of further investigation into its role in SCLC metabolism and could lead to insights into metabolic targeting approaches.

Methods: We investigated differences in metabolic pathways in transcriptional and metabolomics datasets based on cMYC expression in patient and cell line samples. Read More

View Article and Full-Text PDF
September 2021

Coordination of consolidated bioprocessing technology and carbon dioxide fixation to produce malic acid directly from plant biomass in Myceliophthora thermophila.

Biotechnol Biofuels 2021 Sep 23;14(1):186. Epub 2021 Sep 23.

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

Background: Consolidated bioprocessing (CBP) technique is a promising strategy for biorefinery construction, producing bulk chemicals directly from plant biomass without extra hydrolysis steps. Fixing and channeling CO into carbon metabolism for increased carbon efficiency in producing value-added compounds is another strategy for cost-effective bio-manufacturing. It has not been reported whether these two strategies can be combined in one microbial platform. Read More

View Article and Full-Text PDF
September 2021

Astaxanthin overproduction of Phaffia rhodozyma PR106 under titanium dioxide stress by transcriptomics and metabolic regulation analysis.

Bioresour Technol 2021 Sep 15;342:125957. Epub 2021 Sep 15.

College of Food Science and Engineering, Jilin Agricultural University, Changchun, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China; National Engineering Laboratory for Wheat and Corn Deep Processing, Changchun, China. Electronic address:

In this study, astaxanthin yield of Phaffia rhodozyma PR106 increased significantly under titanium dioxide (TiO) stress, and the yield of lycopene and β-carotene also increased significantly, as well as the yield of violaxanthin and lutein significantly decreased; in addition, TiO stress promoted cell division and changed cell morphology of PR106. Then, the mechanism of increasing astaxanthin yield was studied by transcriptomics and related metabolic regulation. The results showed that astaxanthin accumulation in PR106 was not directly related to mRNA transcription and post-translational modifications regulation under TiO stress; TiO stress accelerated glucose uptake of yeast, promoted reuse of ethanol, and increased the formation of acetyl-CoA and ATP. Read More

View Article and Full-Text PDF
September 2021

Heme sequestration as an effective strategy for the suppression of tumor growth and progression.

Mol Cancer Ther 2021 Sep 22. Epub 2021 Sep 22.

Biological Sciences, The University of Texas at Dallas

Heme is an essential nutritional, metabolic, and signaling molecule in living organisms. Pathogenic microbes extract heme from hosts to obtain metallonutrient, while heme fuels mitochondrial respiration and ATP generation in lung tumor cells. Here, we generated small heme-sequestering proteins (HeSPs) based on bacterial hemophores. Read More

View Article and Full-Text PDF
September 2021

KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis.

Mol Cell 2021 Sep 14. Epub 2021 Sep 14.

Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address:

AKT is a serine/threonine kinase that plays an important role in metabolism, cell growth, and cytoskeletal dynamics. AKT is activated by two kinases, PDK1 and mTORC2. Although the regulation of PDK1 is well understood, the mechanism that controls mTORC2 is unknown. Read More

View Article and Full-Text PDF
September 2021

Deletion of pleiotrophin impairs glucose tolerance and liver metabolism in pregnant mice: Moonlighting role of glycerol kinase.

FASEB J 2021 Oct;35(10):e21911

Department of Chemistry and Biochemistry, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Alcorcón, Spain.

Pleiotrophin is a pleiotropic cytokine that has been demonstrated to have a critical role in regulating energy metabolism, lipid turnover and plasticity of adipose tissue. Here, we hypothesize that this cytokine can be involved in regulatory processes of glucose and lipid homeostasis in the liver during pregnancy. Using 18-days pregnant Ptn-deficient mice, we evaluated the biochemical profile (circulating variables), tissue mRNA expression (qPCR) and protein levels of key enzymes and transcription factors involved in main metabolic pathways. Read More

View Article and Full-Text PDF
October 2021

Vanillin modulates activities linked to dysmetabolism in psoas muscle of diabetic rats.

Sci Rep 2021 Sep 21;11(1):18724. Epub 2021 Sep 21.

Department of Biochemistry, University of KwaZulu-Natal, Westville Campus, Durban, 4000, South Africa.

Skeletal muscles are important in glucose metabolism and are affected in type 2 diabetes (T2D) and its complications. This study investigated the effect of vanillin on redox imbalance, cholinergic and purinergic dysfunction, and glucose-lipid dysmetabolism in muscles of rats with T2D. Male albino rats (Sprague-Dawley strain) were fed 10% fructose ad libitum for 2 weeks before intraperitoneally injecting them with 40 mg/kg streptozotocin to induce T2D. Read More

View Article and Full-Text PDF
September 2021

Treatment resistance analysis reveals GLUT-1-mediated glucose uptake as a major target of synthetic rocaglates in cancer cells.

Cancer Med 2021 Sep 20. Epub 2021 Sep 20.

Department of Applied Tumor Biology, Institute of Pathology, University of Heidelberg, Heidelberg, Germany.

Rocaglates are natural compounds that have been extensively studied for their ability to inhibit translation initiation. Rocaglates represent promising drug candidates for tumor treatment due to their growth-inhibitory effects on neoplastic cells. In contrast to natural rocaglates, synthetic analogues of rocaglates have been less comprehensively characterized, but were also shown to have similar effects on the process of protein translation. Read More

View Article and Full-Text PDF
September 2021

Adipocyte-specific mTORC2 deficiency impairs BAT and iWAT thermogenic capacity without affecting glucose uptake and energy expenditure in cold-acclimated mice.

Am J Physiol Endocrinol Metab 2021 Sep 20. Epub 2021 Sep 20.

Department of Physiology and Biophysics, Institute of Biomedical Sciences, grid.11899.38Universidade de São Paulo, São Paulo, Brazil.

Deletion of mTORC2 essential component Rictor by a Cre recombinase under control of the broad, non-adipocyte specific aP2/FABP4 promoter impairs thermoregulation and brown adipose tissue (BAT) glucose uptake upon acute cold exposure. We investigated herein whether adipocyte-specific mTORC2 deficiency affects BAT and inguinal white adipose tissue (iWAT) signaling, metabolism and thermogenesis in cold-acclimated mice. For this, 8-weeks old male mice bearing Rictor deletion and therefore mTORC2 deficiency in adipocytes (adiponectin-Cre) and littermates controls were either kept at thermoneutrality (30 ± 1ºC) or cold-acclimated (10 ± 1ºC) for 14 days and evaluated for BAT and iWAT signaling, metabolism and thermogenesis. Read More

View Article and Full-Text PDF
September 2021

Schistosome AMPK Is Required for Larval Viability and Regulates Glycogen Metabolism in Adult Parasites.

Front Microbiol 2021 1;12:726465. Epub 2021 Sep 1.

Department of Microbiology and Immunology, F. Edward Hébert School of Medicine, Uniformed Services University, Bethesda, MD, United States.

On entering the mammalian host, schistosomes transition from a freshwater environment where resources are scarce, to an environment where there is an unlimited supply of glucose, their preferred energy substrate. Adult schistosome glycolytic activity consumes almost five times the parasite's dry weight in glucose per day to meet the parasite's energy demands, and the schistosome glycolytic enzymes and mechanisms for glucose uptake that sustain this metabolic activity have previously been identified. However, little is known of the parasite processes that regulate schistosome glucose metabolism. Read More

View Article and Full-Text PDF
September 2021

Exposure to Perfluoro-Octanoic Acid Associated With Upstream Uncoupling of the Insulin Signaling in Human Hepatocyte Cell Line.

Front Endocrinol (Lausanne) 2021 3;12:632927. Epub 2021 Sep 3.

Department of Medicine, Unit of Andrology and Reproduction Medicine, University of Padova, Padova, Italy.

Perfluoro-alkyl substances (PFAS) are chemical pollutants with prevalent stability and environmental persistence. Exposure to PFAS, particularly perfluoro-octanoic acid (PFOA), has been associated with increased diabetes-related cardiovascular mortality in subjects residing areas of high environmental contamination, however the exact pathogenic mechanism remains elusive. Here we used HepG2 cells, an model of human hepatocyte, to investigate the possible role of PFOA exposure in the alteration of hepatic glucose metabolism. Read More

View Article and Full-Text PDF
September 2021

Why the diabetic heart is energy inefficient: A ketogenesis and ketolysis perspective.

Am J Physiol Heart Circ Physiol 2021 Sep 17. Epub 2021 Sep 17.

Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, United States.

Lack of glucose uptake compromises metabolic flexibility and reduces energy efficiency in the diabetes mellitus (DM) heart. Although increased utilization of fatty acid to compensate glucose substrate has been studied, less is known about ketone body metabolism in the DM heart. Ketogenic diet reduces obesity, a risk factor for T2DM. Read More

View Article and Full-Text PDF
September 2021

[Myokines and adipomyokines: inflammatory mediators or unique molecules of targeted therapy for obesity?]

Probl Endokrinol (Mosk) 2021 Aug 10;67(4):36-45. Epub 2021 Aug 10.

Endocrinology Research Center.

Skeletal muscles make up about 25% of the total mass in children and more than 40% in adults. Studies of the last twenty years have shown that along with the main functions, muscle tissue has hormonal activity. It was found that myocytes are able to release signaling molecules-myokines. Read More

View Article and Full-Text PDF

[Effect of neuregulin-1 on cardiac glucose metabolism in rats with experimental myocardial infarction].

Zhonghua Xin Xue Guan Bing Za Zhi 2021 Sep;49(9):912-919

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

To investigate the effect of neuregulin-1(NRG-1) on cardiac glucose metabolism in Sprague Dawley (SD) rats with experimental myocardial infarction (MI). Adult male SD rats were randomly divided into three groups: the sham-operated group, MI group, and MI+NRG1 group. The rat MI model was established via ligation of the left anterior descending coronary artery. Read More

View Article and Full-Text PDF
September 2021

Dynamin-related protein 1 regulates substrate oxidation in skeletal muscle by stabilizing cellular and mitochondrial calcium dynamics.

J Biol Chem 2021 Sep 13:101196. Epub 2021 Sep 13.

Integrated Physiology and Molecular Medicine Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA 70808; Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH 44106. Electronic address:

Mitochondria undergo continuous cycles of fission and fusion to promote inheritance, regulate quality control, and mitigate organelle stress. More recently, this process of mitochondrial dynamics has been demonstrated to be highly sensitive to nutrient supply, ultimately conferring bioenergetic plasticity to the organelle. However, whether regulators of mitochondrial dynamics play a causative role in nutrient regulation remains unclear. Read More

View Article and Full-Text PDF
September 2021

Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.

Front Oncol 2021 30;11:733595. Epub 2021 Aug 30.

Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

Background: High glycolysis efficiency in tumor cells can promote tumor growth. lncRNAs play an important role in the proliferation, metabolism and migration of cancer cells, but their regulation of tumor glycolysis is currently not well researched.

Methods: We analyzed the co-expression of glycolysis-related genes and lncRNAs in The Cancer Genome Atlas (TCGA) database to screen glycolysis-related lncRNAs. Read More

View Article and Full-Text PDF

Diagnostic Value of Delayed PET/MR in Liver Metastasis in Comparison With PET/CT.

Front Oncol 2021 30;11:717687. Epub 2021 Aug 30.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), NMPA Key Laboratory for Research and Evaluation of Radiopharmaceuticals (National Medical Products Administration), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, China.

Objectives: The aim of this study was to evaluate the value of a delayed positron emission tomography/magnetic resonance (PET/MR) scan relative to a single positron emission tomography/computed tomography (PET/CT) scan for liver metastasis detection.

Methods: In this study, 70 patients with solid malignancies and suspicious liver lesions undergoing 2-deoxy-2-[F]fluoro--glucose [(F)FDG] PET/CT and subsequent delayed liver PET/MR scans were analyzed. The histopathological analysis and/or imaging follow-up were performed as the standard of reference. Read More

View Article and Full-Text PDF

Upregulated circRAD18 promotes tumor progression by reprogramming glucose metabolism in papillary thyroid cancer.

Gland Surg 2021 Aug;10(8):2500-2510

Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, China.

Background: By regulating complex functional processes, circRNAs are crucial in the development of different cancers. Nevertheless, most circRNAs in papillary thyroid cancer metabolic reprogramming remain unknown.

Methods: The expression of circRNA was assessed by qRT-PCR in papillary thyroid cancer tissues and cell lines. Read More

View Article and Full-Text PDF

NADPH debt drives redox bankruptcy: SLC7A11/xCT-mediated cystine uptake as a double-edged sword in cellular redox regulation.

Genes Dis 2021 Nov 25;8(6):731-745. Epub 2020 Nov 25.

Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Cystine/glutamate antiporter solute carrier family 7 member 11 (SLC7A11; also known as xCT) plays a key role in antioxidant defense by mediating cystine uptake, promoting glutathione synthesis, and maintaining cell survival under oxidative stress conditions. Recent studies showed that, to prevent toxic buildup of highly insoluble cystine inside cells, cancer cells with high expression of SLC7A11 (SLC7A11) are forced to quickly reduce cystine to more soluble cysteine, which requires substantial NADPH supply from the glucose-pentose phosphate pathway (PPP) route, thereby inducing glucose- and PPP-dependency in SLC7A11 cancer cells. Limiting glucose supply to SLC7A11 cancer cells results in significant NADPH "debt", redox "bankruptcy", and subsequent cell death. Read More

View Article and Full-Text PDF
November 2021

Circular RNA ZNF609 enhances proliferation and glycolysis during glioma progression by miR-378b/SLC2A1 axis.

Aging (Albany NY) 2021 09 14;13(17):21122-21133. Epub 2021 Sep 14.

Department of Urology Surgery, Linyi People's Hospital, Linyi, Shandong Province, China.

Glioma is a prevalent brain malignancy with aggressive progression and with grave prognosis in adults. Circular RNAs have been reported to regulate glioma development and function as the diagnostic, prognostic, and therapeutic biomarkers. In this study, we were interested the function of circular RNA ZNF609 in modulating glioma. Read More

View Article and Full-Text PDF
September 2021

Design and evaluation of glutathione responsive glycosylated camptothecin nanosupramolecular prodrug.

Drug Deliv 2021 Dec;28(1):1903-1914

Department of Pharmaceutics, Daqing Campus of Harbin Medical University, Daqing, China.

A novel tumor-targeted glutathione responsive Glycosylated-Camptothecin nanosupramolecular prodrug (CPT-GL NSp) was designed and fabricated a disulfide bond. The effects of glycoligand with different polarities on solubility, self-assembly, stability, cellular uptake, and glutathione responsive cleaving were explored, and an optimal glycosylated ligand was selected for nanosupramolecular prodrug. It has been found that CPT-GL NSp exhibited higher drug loading than traditional nanoparticles. Read More

View Article and Full-Text PDF
December 2021

Ketamine ameliorates depressive-like behaviors in mice through increasing glucose uptake regulated by the ERK/GLUT3 signaling pathway.

Sci Rep 2021 Sep 13;11(1):18181. Epub 2021 Sep 13.

Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, No. 25, Taiping Road, Luzhou, 646000, Sichuan Province, People's Republic of China.

To investigate the effects of ketamine on glucose uptake and glucose transporter (GLUT) expression in depressive-like mice. After HA1800 cells were treated with ketamine, 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino]-2-Deoxyglucose (2-NBDG) was added to the cells to test the effects of ketamine on glucose uptake, production of lactate, and expression levels of GLUT, ERK1/2, AKT, and AMPK. Adult female C57BL/6 mice were subjected to chronic unpredictable mild stress (CUMS), 27 CUMS mice were randomly divided into the depression, ketamine (i. Read More

View Article and Full-Text PDF
September 2021

Predictors of attendance at diabetic retinopathy screening among people with type 2 diabetes: Secondary analysis of data from primary care.

Prim Care Diabetes 2021 Sep 10. Epub 2021 Sep 10.

School of Public Health, University College Cork, Ireland.

Aims: To estimate the uptake of diabetic retinopathy screening among adults with type 2 diabetes and to identify and compare factors associated with attendance at the national diabetic retinal screening programme, and other screening services provided by ophthalmic surgeons, community ophthalmologists and optometrists.

Methods: An observational retrospective cohort study was carried out using data from the 2016 audit of care delivered by general practices (n = 30) enrolled in a structured diabetes care initiative. Attendance at any screening in the previous 12 months, and attendance across different types of service (national programme and other screening services) was calculated. Read More

View Article and Full-Text PDF
September 2021

Emerging roles of kisspeptin/galanin in age-related metabolic disease.

Mech Ageing Dev 2021 Sep 10;199:111571. Epub 2021 Sep 10.

Key Laboratory for Metabolic Diseases in Chinese Medicine, First College of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. Electronic address:

Age is a major risk factor for developing metabolic diseases such as obesity and diabetes. There is an unprecedented rise in obesity and type 2 diabetes in recent decades. A convincing majority of brain-gut peptides are associated with a higher risk to develop metabolic disorders, and may contribute to the pathophysiology of age-related metabolic diseases. Read More

View Article and Full-Text PDF
September 2021

IL-10 Dysregulation Underlies Chemokine Insufficiency, Delayed Macrophage Response, and Impaired Healing in Diabetic Wound.

J Invest Dermatol 2021 Sep 10. Epub 2021 Sep 10.

Department of Medicine, Rush University Medical Center, Chicago, IL, USA; Division of Hematology/Oncology/Cell Therapy, Rush University Medical Center, Chicago, IL, USA; Department of Microbial Pathogens and Immunity, Rush University Medical Center, Chicago, IL, USA; Cancer Center, Rush University Medical Center, Chicago, IL, USA. Electronic address:

Persistent inflammation is a major contributor to healing impairment in diabetic chronic wounds. Paradoxically, diabetic wound environment during the acute phase of healing is completely different in that it exhibits reduced macrophage response due to inadequate expression of CCL2 proinflammatory cytokine. What causes reduction in CCL2 expression in diabetic wound early after injury remains unknown. Read More

View Article and Full-Text PDF
September 2021