2,897 results match your criteria glass beads

Bioactive glass S53P4 to fill-up large cavitary bone defect after acute and chronic osteomyelitis treated with antibiotic-loaded cement beads: A prospective case series with a minimum 2-year follow-up.

Injury 2021 Jun 1. Epub 2021 Jun 1.

Trauma Service, Instituto de Ortopedia e Traumatologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, São Paulo, SP, Brazil.

Introduction: Bioactive glass S53P4 (BAG-S53P4) has been used in the treatment of osteomyelitis with excellent results. The aim of this study was to evaluate the clinical and radiographic results of patients treated with use of antibiotic-loaded cement beads, followed by bone defects filling using bioglass.

Methods: We treated a prospective series of patients presenting with acute or chronic osteomyelitis of a long bone of the upper or lower limb. Read More

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Optical Trapping with Focused Surface Waves.

Ann Phys 2020 Apr 24;532(4). Epub 2020 Feb 24.

Department of Optics and Optical Engineering, Institute of Photonics, University of Science and Technology of China, Hefei, Anhui 230026, China.

Near-field optical trapping can be realized with focused evanescent waves that are excited at the water-glass interface due to the total internal reflection, or with focused plasmonic waves excited on the water-gold interface. Herein, the performance of these two kinds of near-field optical trapping techniques is compared using the same optical microscope configuration. Experimental results show that only a single-micron polystyrene bead can be trapped by the focused evanescent waves, whereas many beads are simultaneously attracted to the center of the excited region by focused plasmonic waves. Read More

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Study on swelling and drug releasing behaviors of ibuprofen-loaded bimetallic alginate aerogel beads with pH-responsive performance.

Colloids Surf B Biointerfaces 2021 Jun 2;205:111895. Epub 2021 Jun 2.

Special Glass Key Lab of Hainan Province, School of Materials Science and Engineering, Hainan University, Haikou 570228, China. Electronic address:

Bimetallic alginate aerogel beads were prepared by ionotropic gelation method with Ca-Ba bimetallic solution and ibuprofen was loaded as a model drug. The swelling and drug releasing behaviors of the beads, especially the influence of barium, were investigated in artificial gastric and intestinal fluids. The results showed that these beads presented higher encapsulation efficiency due to the special structure of aerogel, and barium was beneficial for the more stable structure and drug releasing behavior. Read More

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Preparation and osteogenic properties of nanocomposite hydrogel beads loaded with nanometric bioactive glass particles.

Biomed Mater 2021 Jun 2. Epub 2021 Jun 2.

University of Chile, olivos 943, Santiago de Chile, 1025000, CHILE.

Bone reconstruction in the oral and maxillofacial region presents particular challenges related to the development of biomaterials with osteoinductive properties and suitable physical characteristics for their surgical use in irregular bony defects. In this work, the preparation and bioactivity of chitosan - gelatin (ChG) hydrogel beads loaded with either bioactive glass nanoparticles (nBG) or mesoporous bioactive glass nanospheres (nMBG) were studied. In vitro testing of the bionanocomposite beads was carried out in simulated body fluid (SBF), and through viability and osteogenic differentiation assays using dental pulp stem cells (DPSCs). Read More

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Miniature Optical Particle Counter and Analyzer Involving a Fluidic-Optronic CMOS Chip Coupled with a Millimeter-Sized Glass Optical System.

Sensors (Basel) 2021 May 3;21(9). Epub 2021 May 3.

Institut des Nanotechnologies de Lyon, UMR CNRS 5270 Ecole Centrale de Lyon, Université de Lyon, F-69134 Ecully, France.

Our latest advances in the field of miniaturized optical PM sensors are presented. This sensor combines a hybrid fluidic-optronic CMOS (holed retina) that is able to record a specific irradiance pattern scattered by an illuminated particle (scattering signature), while enabling the circulation of particles toward the sensing area. The holed retina is optically coupled with a monolithic, millimeter-sized, refracto-reflective optical system. Read More

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Microfluidic Magnetic Analyte Delivery Technique for Separation, Enrichment, and Fluorescence Detection of Ultratrace Biomarkers.

Anal Chem 2021 Jun 1;93(23):8273-8280. Epub 2021 Jun 1.

School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China.

A microfluidic magnetic analyte delivery (μMAD) technique was developed to realize sample preparation and ultrasensitive biomarker detection. A simply designed microfluidic device was employed to carry out this technique, including a poly(dimethylsiloxane)-glass hybrid microchip having four straight rectangular channels and a permanent magnet. In the μMAD process, functionalized magnetic beads (MBs) were used to recognize and isolate analytes from a complex sample matrix, deliver analytes into tiny microchannels, and preconcentrate analytes in the magnetic trapping/detection region for in situ fluorescence detection. Read More

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Measuring and upscaling micromechanical interactions in a cohesive granular material.

Soft Matter 2021 May 25. Epub 2021 May 25.

School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.

The mechanical properties of a disordered heterogeneous medium depend, in general, on a complex interplay between multiple length scales. Connecting local interactions to macroscopic observables, such as stiffness or fracture, is thus challenging in this type of material. Here, we study the properties of a cohesive granular material composed of glass beads held together by soft polymer bridges. Read More

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A Simple Technology for Generating Marker-Free Chloroplast Transformants of the Green Alga Chlamydomonas reinhardtii.

Methods Mol Biol 2021 ;2317:293-304

Department of Structural and Molecular Biology, University College London, London, UK.

The availability of routine methods for the genetic engineering of the chloroplast genome of Chlamydomonas reinhardtii is allowing researchers to explore the use of this microalga as a phototrophic cell platform for synthesis of high value recombinant proteins and metabolites. However, the established method for delivering transforming DNA into the algal chloroplast involves microparticle bombardment using an expensive "gene gun". Furthermore, selection of transformant lines most commonly involves the use of a bacterial antibiotic resistance gene. Read More

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January 2021

Visible light photocatalytic bismuth oxide coatings are effective at suppressing aquatic cyanobacteria and degrading free-floating genomic DNA.

J Environ Sci (China) 2021 Jun 18;104:128-136. Epub 2020 Dec 18.

Surface Engineering Group, Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, M1 5GD, UK.

Access to safe drinking water free from microbial pollution is an issue of global concern. The use of photocatalytic thin films in water treatment has focused on titanium dioxide, which requires UV-activation, proving a potential barrier to upscaling and implementation in the real world. Visible-light-activated photocatalytic thin films, such as bismuth oxide, have recently been shown to have antimicrobial properties. Read More

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A simple magnetic-assisted microfluidic method for rapid detection and phenotypic characterization of ultralow concentrations of bacteria.

Talanta 2021 Aug 22;230:122291. Epub 2021 Mar 22.

Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Zhunan, Miaoli, 35053, Taiwan; Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu, 30013, Taiwan; Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung, 40227, Taiwan. Electronic address:

Isolation and enumeration of bacteria at ultralow concentrations and antibiotic resistance profiling are of great importance for early diagnosis and treatment of bacteremia. In this work, we describe a simple, rapid, and versatile magnetic-assisted microfluidic method for rapid bacterial detection. The developed method enables magnetophoretic loading of bead-captured bacteria into the microfluidic chamber under external static and dynamic magnetic fields in 4 min. Read More

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Evaluation of Bonding Gap Control Methods for an Epoxy Adhesive Joint of Carbon Fiber Tubes and Aluminum Alloy Inserts.

Materials (Basel) 2021 Apr 15;14(8). Epub 2021 Apr 15.

Institute of Aeronautics and Applied Mechanics, Warsaw University of Technology, 00-665 Warsaw, Poland.

The aim of this paper is to compare two methods of epoxy adhesive bond gap control: one with a geometrical (mechanical) solution and the other with glass beads, which have the diameter of the desired bond gap and are mixed with an epoxy adhesive. The adhered materials were carbon fiber composite tubes and aluminum alloy inserts, which were used as wishbones in a suspension system of a motorsport vehicle. It was assumed that the gap thickness would be equal to 0. Read More

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Effect of Air-Particle Abrasion Protocol and Primer on The Topography and Bond Strength of a High-Translucent Zirconia Ceramic.

J Prosthodont 2021 Apr 28. Epub 2021 Apr 28.

Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Japan.

Purpose: To evaluate effect of air-particle abrasion protocol and primer on surface topography and bond strength of resin cement to high-translucent zirconia ceramics.

Materials And Methods: Two hundred disk-shaped high-translucent zirconia specimens of 5Y-PSZ were prepared. The specimens were assigned to 5 groups in terms of particle type and air-particle abrasion pressure: (1) control, (2) alumina with 0. Read More

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Development of robust models for rapid classification of microplastic polymer types based on near infrared hyperspectral images.

Anal Methods 2021 May;13(19):2215-2222

Marine Biodiversity and Environmental Assessment Research Center (BioEnv), Research Institute for Global Change (RIGC), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.

Hyperspectral data in the near infrared range were examined for nine common types of plastic particles of 1 mm and 100-500 μm sizes on dry and wet glass fiber filters. Weaker peak intensities were detected for small particles compared to large particles, and the reflectances were weaker at longer wavelengths when the particles were measured on a wet filter. These phenomena are explainable due to the effect of the correlation between the particle size and the absorption of infrared light by water. Read More

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A modular microscopic smartphone attachment for imaging and quantification of multiple fluorescent probes using machine learning.

Analyst 2021 Apr;146(8):2531-2541

Department of Electrical and Computer Engineering, School of Engineering, Rutgers The State University of New Jersey, Piscataway, NJ, USA. and Global Health Institute, Rutgers The State University of New Jersey, New Brunswick, NJ, USA.

Portable smartphone-based fluorescent microscopes are becoming popular owing to their ability to provide major functionalities offered by regular benchtop microscopes at a fraction of the cost. However, smartphone-based microscopes are still limited to a single fluorophore, fixed magnification, the inability to work with a different smartphones, and limited usability to either glass slides or cover slips. To overcome these challenges, here we present a modular smartphone-based microscopic attachment. Read More

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Glass-like characteristics of intracellular motion in human cells.

Biophys J 2021 Jun 19;120(11):2355-2366. Epub 2021 Apr 19.

Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands.

The motion in the cytosol of microorganisms such as bacteria and yeast has been observed to undergo a dramatic slowing down upon cell energy depletion. These observations have been interpreted as the motion being "glassy," but whether this notion is useful also for active, motor-protein-driven transport in eukaryotic cells is less clear. Here, we use fluorescence microscopy of beads in human (HeLa) cells to probe the motion of membrane-surrounded structures that are carried along the cytoskeleton by motor proteins. Read More

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An integrated actuating and sensing system for light-addressable potentiometric sensor (LAPS) and light-actuated AC electroosmosis (LACE) operation.

Biomicrofluidics 2021 Mar 12;15(2):024109. Epub 2021 Apr 12.

Ph.D. Program in Biomedical Engineering, Chang Gung University, Taoyuan City 333, Taiwan.

To develop a lab on a chip (LOC) integrated with both sensor and actuator functions, a novel two-in-one system based on optical-driven manipulation and sensing in a microfluidics setup based on a hydrogenated amorphous silicon (a-Si:H) layer on an indium tin oxide/glass is first realized. A high-intensity discharge xenon lamp functioned as the light source, a chopper functioned as the modulated illumination for a certain frequency, and a self-designed optical path projected on the digital micromirror device controlled by the digital light processing module was established as the illumination input signal with the ability of dynamic movement of projected patterns. For light-addressable potentiometric sensor (LAPS) operation, alternating current (AC)-modulated illumination with a frequency of 800 Hz can be generated by the rotation speed of the chopper for photocurrent vs bias voltage characterization. Read More

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Transport of nanoparticles in porous media and its effects on the co-existing pollutants.

Environ Pollut 2021 Aug 7;283:117098. Epub 2021 Apr 7.

Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing, 210098, China.

Nanomaterials are widely used in daily life owing to their superior characteristics. The release and transport of nanoparticles (NPs) in the environment is inevitable during their entire life cycle, posing a risk to the aquatic environment. Thus, considerable attention has been focused on the fate and behavior of NPs in porous media, as well as the co-transport of NPs with other pollutants. Read More

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A modified culture medium and hyphae isolation method can increase quality of the RNA extracted from mycelia of a dimorphic fungal species.

Curr Genet 2021 Apr 10. Epub 2021 Apr 10.

Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, 4032, Debrecen, Hungary.

The capability of RNA isolation with good efficiency and high quality is essential for a downstream application such as RNA sequencing. It requires successful cell culturing and an effective RNA isolation method. Although effective methods are available, production of the homogenous mycelia and extraction of good-quality mycelial RNA from true invasive hyphae, which penetrated into the agar plates, are difficult. Read More

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Post-Processing and Surface Characterization of Additively Manufactured Stainless Steel 316L Lattice: Implications for BioMedical Use.

Materials (Basel) 2021 Mar 12;14(6). Epub 2021 Mar 12.

Department of Orthopaedic Surgery, National University Hospital Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore.

Additive manufacturing of stainless steel is becoming increasingly accessible, allowing for the customisation of structure and surface characteristics; there is little guidance for the post-processing of these metals. We carried out this study to ascertain the effects of various combinations of post-processing methods on the surface of an additively manufactured stainless steel 316L lattice. We also characterized the nature of residual surface particles found after these processes via energy-dispersive X-ray spectroscopy. Read More

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Microcapillary Reactors via Coaxial Electrospinning: Fabrication of Small Poly(Acrylic Acid) Gel Beads and Thin Threads of Biological Cell Dimensions.

Gels 2021 Mar 30;7(2). Epub 2021 Mar 30.

Department of Macromolecular Science and Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.

Poly(acrylic acid) (PAA) bulk gels and threads, typically derived via free-radical polymerization, are of interest as anionic polyelectrolyte mimics of cellular cytosol and as models for early protocells. The thread dimensions have been limited by the diameters of readily-available glass or plastic capillaries, and threads with diameters of less than 50 µm have been difficult to achieve. Here, we report a useful approach for achieving crosslinked, partially neutralized PAA, namely poly(acrylate), gel threads with diameters of a few microns when dry. Read More

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Adding further evidence for clinically significant anti-Le antibody in a voluntary blood donor.

Asian J Transfus Sci 2020 Jul-Dec;14(2):198-199. Epub 2020 Dec 19.

Fatima Hospital, Gorakhpur, Uttar Pradesh, India.

Herein, we report a case of naturally occurring anti-Le alloantibody identified in the plasma of a first time voluntary blood donor. The immunohematology workup was done on the pilot sample tubes collected during blood donation by the conventional tube technique and using ID-Micro Column System Glass Beads card (anti-IgG, Cd; Ortho-Clinical Diagnostics, Raritan, New Jersey, USA). Blood group of the donor was confirmed to be B RhD positive, and the alloantibody in his plasma was identified as anti-Le, having clinically significant characteristics. Read More

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December 2020

A literature survey of all volatiles from healthy human breath and bodily fluids: the human volatilome.

J Breath Res 2021 Apr 21;15(3). Epub 2021 Apr 21.

Centre of Research in Biosciences, University of the West of England, Frenchay Campus, Coldharbour Lane, Bristol BS16 1QY, United Kingdom.

This paper comprises an updated version of the 2014 review which reported 1846 volatile organic compounds (VOCs) identified from healthy humans. In total over 900 additional VOCs have been reported since the 2014 review and the VOCs from semen have been added. The numbers of VOCs found in breath and the other bodily fluids are: blood 379, breath 1488, faeces 443, milk 290, saliva 549, semen 196, skin 623 and urine 444. Read More

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Sustainable functionalized metal-organic framework NH-MIL-101(Al) for CO separation under cryogenic conditions.

Environ Pollut 2021 Jun 10;279:116924. Epub 2021 Mar 10.

Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia. Electronic address:

In this study, a sustainable NH-MIL-101(Al) is synthesized and subjected to characterization for cryogenic CO adsorption, isotherms, and thermodynamic study. The morphology revealed a highly porous surface. The XRD showed that NH-MIL-101(Al) was crystalline. Read More

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Efficient Entrapment of Carbonic Anhydrase in Alginate Hydrogels Using Liposomes for Continuous-Flow Catalytic Reactions.

ACS Omega 2021 Mar 22;6(9):6368-6378. Epub 2021 Feb 22.

Department of Applied Chemistry, Yamaguchi University, Tokiwadai 2-16-1, Ube 755-8611, Japan.

A versatile approach to entrap relatively small enzymes in hydrogels allows their diverse biotechnological applications. In the present work, bovine carbonic anhydrase (BCA) was efficiently entrapped in calcium alginate beads with the help of liposomes. A mixture of sodium alginate (3 wt %) and carbonic anhydrase-liposome conjugates (BCALs) was dripped into a Tris-HCl buffer solution (pH = 7. Read More

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Paper-thin multilayer microfluidic devices with integrated valves.

Lab Chip 2021 04 10;21(7):1287-1298. Epub 2021 Mar 10.

Ningbo University, College of Food and Pharmaceutical Sciences, Ningbo, Zhejiang 315832, China.

Integrated valve microfluidics has an unparalleled capability to automate rapid delivery of fluids at the nanoliter scale for high-throughput biological experimentation. However, multilayer soft lithography, which is used to fabricate valve-microfluidics, produces devices with a minimum thickness of around five millimeters. This form-factor limitation prevents the use of such devices in experiments with limited sample thickness tolerance such as 4-pi microscopy, stimulated Raman scattering microscopy, and many forms of optical or magnetic tweezer applications. Read More

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Microbiota functional activity biosensors for characterizing nutrient metabolism in vivo.

Elife 2021 Mar 8;10. Epub 2021 Mar 8.

Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, United States.

Methods for measuring gut microbiota biochemical activities in vivo are needed to characterize its functional states in health and disease. To illustrate one approach, an arabinan-containing polysaccharide was isolated from pea fiber, its structure defined, and forward genetic and proteomic analyses used to compare its effects, versus unfractionated pea fiber and sugar beet arabinan, on a human gut bacterial strain consortium in gnotobiotic mice. We produced 'Microbiota Functional Activity Biosensors' (MFABs) consisting of glycans covalently linked to the surface of fluorescent paramagnetic microscopic glass beads. Read More

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Photodegradation of organic water pollutants under visible light using anatase F, N co-doped TiO/SiO nanocomposite: Semi-pilot plant experiment and density functional theory calculations.

Chemosphere 2021 Jul 19;275:129903. Epub 2021 Feb 19.

Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran. Electronic address:

Visible-light driven photocatalysts are of great importance in wastewater treatment. In this work, fluorine and nitrogen co-doped titanium dioxide/silica nanocomposite (F-N-TiO/SiO) was synthetized using a sol-gel approach. The as-developed nanocomposite was well characterized using different techniques. Read More

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Alternative tehcniques for Xanthomonas arboricola pv pruni preservation at room temperature.

J Microbiol Methods 2021 04 19;183:106173. Epub 2021 Feb 19.

Technological Development Center, Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil; Department of Food Science and Technology, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil; Chemical, Pharmaceutical and Food Sciences Center, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil. Electronic address:

The efficiency of alternative preservation techniques for Xanthomonas arboricola pv pruni was studied. The preservation methods in sunflower seeds, glass beads and sterile soil were suitable for maintaining viability and productive capacity of xanthan pruni. Read More

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Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis.

J Vis Exp 2021 02 6(168). Epub 2021 Feb 6.

Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center; Department of Ophthalmology, Dean McGee Eye Institute; Dean McGee Eye Institute;

Intraocular bacterial infections are a danger to the vision. Researchers use animal models to investigate the host and bacterial factors and immune response pathways associated with infection to identify viable therapeutic targets and to test drugs to prevent blindness. The intravitreal injection technique is used to inject organisms, drugs, or other substances directly into the vitreous cavity in the posterior segment of the eye. Read More

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February 2021

Activity, stability, and binding capacity of β-galactosidase immobilized on electrospun nylon-6 fiber membrane.

J Dairy Sci 2021 Apr 13;104(4):3888-3898. Epub 2021 Feb 13.

Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT 84602. Electronic address:

In this research, we explored various immobilized enzyme support materials, including the novel nylon-6 fiber membrane (NFM), and evaluated the increase in surface area and its effect on enzyme binding potential. We also manipulated incubation and reaction conditions and assessed the subsequent effects on activity and stability of β-galactosidase, with comparisons between various solid support materials and free (dissolved) enzyme. Nylon-6 fiber membranes were created by electrospinning and were compared with other materials as solid supports for enzyme binding. Read More

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