160 results match your criteria gigaspora margarita


Soil legacy of arbuscular mycorrhizal fungus Gigaspora margarita: The potassium-sequestering glomalin improves peanut (Arachis hypogaea) drought resistance and pod yield.

Microbiol Res 2021 Aug 29;249:126774. Epub 2021 Apr 29.

Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, China. Electronic address:

In agroecosystems, drought stress severely threatens crops development. Although potassium (K) is required in amounts by crops under drought stress, the mobilization and availablity of K are limited by the soil water status. Arbuscular mycorrhizal (AM) fungi can form mutualistic associations with most crops and play direct or indirect roles in the host drought resistance. Read More

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The Mosaic Architecture of NRPS-PKS in the Arbuscular Mycorrhizal Fungus Shows a Domain With Bacterial Signature.

Front Microbiol 2020 26;11:581313. Epub 2020 Nov 26.

Department of Life Sciences and Systems Biology, University of Turin, Turin, Italy.

As obligate biotrophic symbionts, arbuscular mycorrhizal fungi (AMF) live in association with most land plants. Among them, has been deeply investigated because of its peculiar features, i.e. Read More

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November 2020

Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes.

mSystems 2020 Dec 15;5(6). Epub 2020 Dec 15.

College of Resources and Environmental Sciences, China Agricultural University, Beijing, China

Plant roots are usually colonized by various arbuscular mycorrhizal (AM) fungal species, which vary in morphological, physiological, and genetic traits. This colonization constitutes the mycorrhizal nutrient uptake pathway (MP) and supplements the pathway through roots. Simultaneously, the extraradical hyphae of each AM fungus is associated with a community of bacteria. Read More

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December 2020

Myristate can be used as a carbon and energy source for the asymbiotic growth of arbuscular mycorrhizal fungi.

Proc Natl Acad Sci U S A 2020 10 30;117(41):25779-25788. Epub 2020 Sep 30.

Faculty of Agriculture, Shinshu University, Minamiminowa, 399-4598 Nagano, Japan

Arbuscular mycorrhizal (AM) fungi, forming symbiotic associations with land plants, are obligate symbionts that cannot complete their natural life cycle without a host. The fatty acid auxotrophy of AM fungi is supported by recent studies showing that lipids synthesized by the host plants are transferred to the fungi, and that the latter lack genes encoding cytosolic fatty acid synthases. Therefore, to establish an asymbiotic cultivation system for AM fungi, we tried to identify the fatty acids that could promote biomass production. Read More

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October 2020

and Its Endobacterium Modulate Symbiotic Marker Genes in Tomato Roots under Combined Water and Nutrient Stress.

Plants (Basel) 2020 Jul 14;9(7). Epub 2020 Jul 14.

Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, I-10125 Torino, Italy.

As members of the plant microbiota, arbuscular mycorrhizal fungi (AMF) may be effective in enhancing plant resilience to drought, one of the major limiting factors threatening crop productivity. AMF host their own microbiota and previous data demonstrated that endobacteria thriving in modulate fungal antioxidant responses. Here, we used the - Glomeribacter gigasporarum system to test whether the tripartite interaction between tomato, and its endobacteria may improve plant resilience to combined water/nutrient stress. Read More

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Effects of single and multiple species inocula of arbuscular mycorrhizal fungi on the salinity tolerance of a Bangladeshi rice (Oryza sativa L.) cultivar.

Mycorrhiza 2020 Jul 4;30(4):431-444. Epub 2020 May 4.

Department of Biology, Plant Conservation and Population Biology, KU Leuven, Kasteelpark Arenberg 31, 2435, 3001, Heverlee, Belgium.

Soil salinization due to sea level rise and groundwater irrigation has become an important agronomic problem in many parts of the world. Symbiosis between crop species and arbuscular mycorrhizal fungi (AMF) may alleviate salt stress-induced detrimental effects on crop growth and yield, for example, through helping the host plant to selectively absorb potassium while avoiding uptake of excessive sodium. Here, we performed a greenhouse experiment to evaluate growth, grain yield, and salt tolerance of a Bangladeshi rice cultivar under three levels of salt stress (0, 75, and 120 mM) after inoculation with three different AMF species from three different genera (Funnelliformis mosseae (BEG12), Acaulospora laevis (BEG13), and Gigaspora margarita (BEG34)), singly and in combination. Read More

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Siderophores in plant root tissue: Tagetes patula nana colonized by the arbuscular mycorrhizal fungus Gigaspora margarita.

Biometals 2020 06 3;33(2-3):137-146. Epub 2020 May 3.

Department of Microbiology & Biotechnology, University of Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany.

More than 70% of vascular plant species live in symbiosis with arbuscular mycorrhizal (AM) fungi. In addition to other effects this symbiosis is known for its significance for plant nutrition including iron. Fungal iron mobilization from soil is commonly dependent on siderophores. Read More

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Evaluation of secondary ions related to plant tissue using least absolute shrinkage and selection operator.

Biointerphases 2020 04 9;15(2):021010. Epub 2020 Apr 9.

Faculty of Science and Technology, Seikei University, 3-3-1 Kichijoji-Kitamachi, Musashino-shi, Tokyo 180-8633, Japan.

With regard to life sciences, it is important to understand biological functions such as metabolic reactions at the cellular level. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) that can provide chemical mappings at 100 nm lateral resolutions is useful for obtaining three-dimensional maps of biological molecules in cells and tissues. TOF-SIMS spectra generally contain several hundred to several thousand secondary ion peaks that provide detailed chemical information. Read More

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Different Genetic Sources Contribute to the Small RNA Population in the Arbuscular Mycorrhizal Fungus .

Front Microbiol 2020 13;11:395. Epub 2020 Mar 13.

Department of Life Sciences and Systems Biology, School of Nature Sciences, University of Turin, Turin, Italy.

RNA interference (RNAi) is a key regulatory pathway of gene expression in almost all eukaryotes. This mechanism relies on short non-coding RNA molecules (sRNAs) to recognize in a sequence-specific manner DNA or RNA targets leading to transcriptional or post-transcriptional gene silencing. To date, the fundamental role of sRNAs in the regulation of development, stress responses, defense against viruses and mobile elements, and cross-kingdom interactions has been extensively studied in a number of biological systems. Read More

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At the nexus of three kingdoms: the genome of the mycorrhizal fungus Gigaspora margarita provides insights into plant, endobacterial and fungal interactions.

Environ Microbiol 2020 01 31;22(1):122-141. Epub 2019 Oct 31.

Department of Life Sciences and Systems Biology, University of Turin, Turin, Italy.

As members of the plant microbiota, arbuscular mycorrhizal fungi (AMF, Glomeromycotina) symbiotically colonize plant roots. AMF also possess their own microbiota, hosting some uncultivable endobacteria. Ongoing research has revealed the genetics underlying plant responses to colonization by AMF, but the fungal side of the relationship remains in the dark. Read More

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January 2020

Size matters: three methods for estimating nuclear size in mycorrhizal roots of Medicago truncatula by image analysis.

BMC Plant Biol 2019 May 4;19(1):180. Epub 2019 May 4.

Department of Life Sciences and Systems Biology, University of Turin, 10125, Torino, Italy.

Background: The intracellular accommodation of arbuscular mycorrhizal (AM) fungi involves a profound molecular reprogramming of the host cell architecture and metabolism, based on the activation of a symbiotic signaling pathway. In analogy with other plant biotrophs, AM fungi are reported to trigger cell cycle reactivation in their host tissues, possibly in support of the enhanced metabolic demand required for the symbiosis.

Results: We here compare the efficiency of three Fiji/ImageJ image analysis plugins in localizing and quantifying the increase in nuclear size - a hallmark of recursive events of endoreduplication - in M. Read More

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Maize varieties can strengthen positive plant-soil feedback through beneficial arbuscular mycorrhizal fungal mutualists.

Mycorrhiza 2019 May 27;29(3):251-261. Epub 2019 Mar 27.

Soil Biology Group, Wageningen University & Research, P.O. Box 47, 6700 AA, Wageningen, The Netherlands.

Plant-soil feedback (PSF) describes the process whereby plant species modify the soil environment, which subsequently impacts the growth of the same or another plant species. Our aim was to explore PSF by two maize varieties (a landrace and a hybrid variety) and three arbuscular mycorrhizal fungi (AMF) species (Funneliformis mosseae, Claroideoglomus etunicatum, Gigaspora margarita, and the mixture). We carried out a pot experiment with a conditioning and a feedback phase to determine PSF with different species of AMF and with a non-mycorrhizal control. Read More

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Local endoreduplication as a feature of intracellular fungal accommodation in arbuscular mycorrhizas.

New Phytol 2019 07 1;223(1):430-446. Epub 2019 Apr 1.

Department of Life Sciences and Systems Biology, University of Turin, 10125, Torino, Italy.

The intracellular accommodation of arbuscular mycorrhizal (AM) fungi is a paradigmatic feature of this plant symbiosis that depends on the activation of a dedicated signaling pathway and the extensive reprogramming of host cells, including striking changes in nuclear size and transcriptional activity. By combining targeted sampling of early root colonization sites, detailed confocal imaging, flow cytometry and gene expression analyses, we demonstrate that local, recursive events of endoreduplication are triggered in the Medicago truncatula root cortex during AM colonization. AM colonization induces an increase in ploidy levels and the activation of endocycle specific markers. Read More

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Landrace maize varieties differ from conventional and genetically modified hybrid maize in response to inoculation with arbuscular mycorrhizal fungi.

Mycorrhiza 2019 May 20;29(3):237-249. Epub 2019 Feb 20.

Rural Engineering Department, Federal University of Santa Catarina, Florianopolis, Brazil.

Land area planted with genetically modified (GM) crops has grown rapidly, and Brazil has the second largest area with those plants. There is, however, limited information on the possible effects of that technology on non-target organisms, especially root symbionts, such as arbuscular mycorrhizal fungi (AMF). We evaluated AMF symbiosis development in five maize genotypes: one landrace, two conventional hybrids (DKB 240 and Formula), and two GM hybrids (DKB 240-VT Pro and Formula TL). Read More

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Metatranscriptomic Analysis and In Silico Approach Identified Mycoviruses in the Arbuscular Mycorrhizal Fungus spp.

Viruses 2018 12 12;10(12). Epub 2018 Dec 12.

Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA.

Arbuscular mycorrhizal fungi (AMF), including spp., can play important roles in nutrient cycling of the rhizosphere. However, the effect of virus infection on AMF's role in nutrient cycling cannot be determined without first knowing the diversity of the mycoviruses in AMF. Read More

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December 2018

Lotuslactone, a non-canonical strigolactone from Lotus japonicus.

Phytochemistry 2019 Jan 12;157:200-205. Epub 2018 Nov 12.

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Nakaku, Sakai, Osaka, 599-8531, Japan; CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0112, Japan. Electronic address:

Root exudates from Lotus japonicus were found to contain at least three different hyphal branching-inducing compounds for the arbuscular mycorrhizal (AM) fungus Gigaspora margarita, one of which had been previously identified as (+)-5-deoxystrigol (5DS), a canonical strigolactone (SL). One of the two remaining unknown hyphal branching inducers was purified and named lotuslactone. Its structure was determined as methyl (E)-2-(3-acetoxy-2-hydroxy-7-methyl-1-oxo-1,2,3,4,5,6-hexahydroazulen-2-yl)-3-(((R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yl)oxy)acrylate, by 1D and 2D NMR spectroscopy, and HR-ESI- and EI-MS. Read More

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January 2019

Effects of Long-Term Subcultured Arbuscular Mycorrhizal Fungi on Red Pepper Plant Growth and Soil Glomalin Content.

Mycobiology 2018 10;46(2):122-128. Epub 2018 May 10.

Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, Republic of Korea.

Arbuscular mycorrhizal fungi (AMF) are well-known for their ability to improve plant growth and help plants withstand abiotic stress conditions. Unlike other fungi and bacteria, AMF cannot be stored, as they are obligate biotrophs. Long-term preservation of AMF spores is challenging and may lead to the loss of viability and efficiency. Read More

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Mycorrhiza-induced alleviation of plant disease caused by Clavibacter michiganensis subsp. michiganensis and role of ethylene in mycorrhiza-induced resistance in tomato.

Acta Biol Hung 2018 Jun;69(2):170-181

Institute of Genetics, Microbiology and Biotechnology, Szent István University , Páter Károly út 1, Gödöllő H-2100 , Hungary.

The protective role of arbuscular mycorrhizal fungi (AMF) against the phytopathogen Clavibacter michiganensis subsp. michiganensis (Cmm) was examined in tomato plants. Seven different AMF isolates were used to determine which ones were able to induce effectively resistance against Cmm. Read More

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Metabolome changes are induced in the arbuscular mycorrhizal fungus Gigaspora margarita by germination and by its bacterial endosymbiont.

Mycorrhiza 2018 Aug 2;28(5-6):421-433. Epub 2018 Jun 2.

Department of Chemistry, University of Tennessee, 1420 Circle Drive, Knoxville, TN, 37996, USA.

Metabolomic profiling is becoming an increasingly important technique in the larger field of systems biology by allowing the simultaneous measurement of thousands of small molecules participating in and resulting from cellular reactions. In this way, metabolomics presents an opportunity to observe the physiological state of a system, which may provide the ability to monitor the whole of cellular metabolism as the technology progresses. The arbuscular mycorrhizal fungus Gigaspora margarita has not previously been explored with regard to metabolite composition. Read More

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The virome of the arbuscular mycorrhizal fungus Gigaspora margarita reveals the first report of DNA fragments corresponding to replicating non-retroviral RNA viruses in fungi.

Environ Microbiol 2018 06 23;20(6):2012-2025. Epub 2018 Feb 23.

Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.

Arbuscular Mycorrhizal Fungi (AMF) are key components of the plant microbiota. AMF genetic complexity is increased by the presence of endobacteria, which live inside many species. A further component of such complexity is the virome associated to AMF, whose knowledge is still very limited. Read More

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Arbuscular mycorrhizal fungi spore propagation using single spore as starter inoculum and a plant host.

J Appl Microbiol 2018 Jun 23;124(6):1556-1565. Epub 2018 Mar 23.

Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk, Korea.

Aims: The propagation of pure cultures of arbuscular mycorrhizal fungal (AMF) is an essential requirement for their large-scale agricultural application and commercialization as biofertilizers. The present study aimed to propagate AMF using the single-spore inoculation technique and compare their propagation ability with the known reference spores.

Methods And Results: Arbuscular mycorrhizal fungal spores were collected from salt-affected Saemangeum reclaimed soil in South Korea. Read More

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Strigolactones cross the kingdoms: plants, fungi, and bacteria in the arbuscular mycorrhizal symbiosis.

J Exp Bot 2018 04;69(9):2175-2188

Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.

Strigolactones (SLs) first evolved as regulators of simple developmental processes in very ancient plant lineages, and then assumed new roles to sustain the increasing biological complexity of land plants. Their versatility is also shown by the fact that during evolution they have been exploited, once released in the rhizosphere, as a communication system towards plant-interacting organisms even belonging to different kingdoms. Here, we reviewed the impact of SLs on soil microbes, paying particular attention to arbuscular mycorrhizal fungi (AMF). Read More

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Gigaspora margarita with and without its endobacterium shows adaptive responses to oxidative stress.

Mycorrhiza 2017 Nov 20;27(8):747-759. Epub 2017 Jul 20.

Department of Life Sciences and Systems Biology, University of Torino, viale Mattioli 25, 10125, Torino, Italy.

Arbuscular mycorrhizal (AM) fungi experience oxidative stress during the plant-fungal interaction, due to endogenous reactive oxygen species (ROS) produced by fungal metabolism and exogenous ROS produced by plant cells. Here, we examine the responses to HO in Gigaspora margarita, an AM fungus containing the endobacterial symbiont Candidatus Glomeribacter gigasporarum (CaGg). Previous studies revealed that G. Read More

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November 2017

Real-time PCR quantification of arbuscular mycorrhizal fungi: does the use of nuclear or mitochondrial markers make a difference?

Mycorrhiza 2017 Aug 31;27(6):577-585. Epub 2017 May 31.

Institute of Botany, The Czech Academy of Sciences, Zámek 1, Průhonice, 252 43, Czech Republic.

Root colonization by arbuscular mycorrhizal fungi (AMF) can be quantified by different approaches. We compared two approaches that enable discrimination of specific AMF taxa and are therefore emerging as alternative to most commonly performed microscopic quantification of AMF in roots: quantitative real-time PCR (qPCR) using markers in nuclear ribosomal DNA (nrDNA) and mitochondrial ribosomal DNA (mtDNA). In a greenhouse experiment, Medicago truncatula was inoculated with four isolates belonging to different AMF species (Rhizophagus irregularis, Claroideoglomus claroideum, Gigaspora margarita and Funneliformis mosseae). Read More

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Population and function analysis of cultivable bacteria associated with spores of arbuscular mycorrhizal fungus Gigaspora margarita.

3 Biotech 2017 May 8;7(1). Epub 2017 Apr 8.

Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology South China, Guangzhou, 510070, China.

This study was aimed to investigate the diversity and function of bacterial population associated with Gigaspora margarita spores. The fungus was propagated in sterilized sand/soil pots using alfalfa (Medicago sativa), grain sorghum (Sorghum bicolor), or maize (Zea mays) as host plants, or in sterilized vermiculite pots using alfalfa as host plants, respectively. Bacteria were isolated from the new-formed spores using diluted plate method, and typical bacterial isolates were identified according to 16S rRNA gene phylogenetic analysis. Read More

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Biocontrol agent f.sp. has no adverse effect on indigenous total fungal communities and specific AMF taxa in contrasting maize rhizospheres.

Fungal Ecol 2016 Oct;23:1-10

Institute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), University of Hohenheim, Stuttgart, Germany.

We studied the effects of f.sp. (Fos), a soil-borne biocontrol agent (BCA) against , on total fungal and arbuscular mycorrhizal fungal (AMF) taxa in rhizospheres of maize in both clayey and sandy soil. Read More

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October 2016

Arbuscular Mycorrhizal Symbiosis Requires a Phosphate Transceptor in the Gigaspora margarita Fungal Symbiont.

Mol Plant 2016 12 27;9(12):1583-1608. Epub 2016 Sep 27.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R.China. Electronic address:

The majority of terrestrial vascular plants are capable of forming mutualistic associations with obligate biotrophic arbuscular mycorrhizal (AM) fungi from the phylum Glomeromycota. This mutualistic symbiosis provides carbohydrates to the fungus, and reciprocally improves plant phosphate uptake. AM fungal transporters can acquire phosphate from the soil through the hyphal networks. Read More

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December 2016

Arbuscular mycorrhizal fungi in the growth and extraction of trace elements by Chrysopogon zizanioides (vetiver) in a substrate containing coal mine wastes.

Int J Phytoremediation 2017 Feb;19(2):113-120

a Biological Sciences Center, Microbiology, Immunology and Parasitology Department, Federal University of Santa Catarina (UFSC) , Florianópolis , Santa Catarina , Brazil.

Vetiver (Chrysopogon zizanioides) is a fast-growing, high biomass producing plant employed for environmental rehabilitation. The study evaluated the effects of arbuscular mycorrhizal fungi (AMF) on the growth and trace element phytoextracting capabilities of vetiver in a substrate containing coalmine wastes in Southern Brazil. AMF included Acaulospora colombiana, Acaulospora morrowiae, Acaulospora scrobiculata, Dentiscutata heterogama, Gigaspora margarita, and Rhizophagus clarus. Read More

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February 2017

Carlactone-type strigolactones and their synthetic analogues as inducers of hyphal branching in arbuscular mycorrhizal fungi.

Phytochemistry 2016 Oct 2;130:90-8. Epub 2016 Jun 2.

Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. Electronic address:

Hyphal branching in the vicinity of host roots is a host recognition response of arbuscular mycorrhizal fungi. This morphological event is elicited by strigolactones. Strigolactones are carotenoid-derived terpenoids that are synthesized from carlactone and its oxidized derivatives. Read More

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October 2016

Sequence variation in nuclear ribosomal small subunit, internal transcribed spacer and large subunit regions of Rhizophagus irregularis and Gigaspora margarita is high and isolate-dependent.

Mol Ecol 2016 06 18;25(12):2816-32. Epub 2016 May 18.

Department of Botany, University of Tartu, 40 Lai St., 51005, Tartu, Estonia.

Arbuscular mycorrhizal (AM) fungi are known to exhibit high intra-organism genetic variation. However, information about intra- vs. interspecific variation among the genes commonly used in diversity surveys is limited. Read More

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