1,360 results match your criteria gfap nestin


Ultrarapid Inflammation of the Olfactory Bulb After Spinal Cord Injury: Protective Effects of the Granulocyte Colony-Stimulating Factor on Early Neurodegeneration in the Brain.

Front Aging Neurosci 2021 25;13:701702. Epub 2021 Jun 25.

Department of Biotechnology and Animal Science, College of Bioresources, National Ilan University, Yilan, Taiwan.

The correlation among olfactory dysfunction, spinal cord injury (SCI), subjective cognitive decline, and neurodegenerative dementia has been established. Impaired olfaction is considered a marker for neurodegeneration. Hence, there is a need to examine if SCI leads to olfactory dysfunction. Read More

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Synergic Neuroprotection Between Hort and Borneol Against Ischemic Stroke by Neurogenesis Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.

Front Pharmacol 2021 16;12:666790. Epub 2021 Jun 16.

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, China.

Hort (LCH) is a famous ethnomedicine in Asia known for its excellent output on stroke treatment, and borneol usually acts as an assistant for its reducing permeability of the blood-brain barrier (BBB) after stroke. Although their synergy against brain ischemia was verified in previous studies, the potential mechanism is still unknown. The research aimed to explore the exact synergic mechanisms between LCH and borneol on neurogenesis within the areas of the dentate gyrus and subventricular zone. Read More

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Differentiation of Porcine Induced Pluripotent Stem Cells (piPSCs) into Neural Progenitor Cells (NPCs).

J Vis Exp 2021 Jun 11(172). Epub 2021 Jun 11.

Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo; Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo;

iPSC-derived neurons are attractive in vitro models to study neurogenesis and early phenotypic changes in mental illness, mainly when most animal models used in pre-clinical research, such as rodents, are not able to meet the criteria to translate the findings to the clinic. Non-human primates, canines, and porcine are considered more adequate models for biomedical research and drug development purposes, mainly due to their physiological, genetic, and anatomical similarities to humans. The swine model has gained particular interest in translational neuroscience, enabling safety and allotransplantation testing. Read More

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Differential expression of stem cell markers in proliferating cells in glioma.

J Cancer Res Clin Oncol 2021 Jun 25. Epub 2021 Jun 25.

Institute of Neuropathology, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany.

Purpose: The identification of prognostically and therapeutically relevant molecular markers is fundamental to the further development of personalised therapies in brain tumours. Current therapeutic options for the treatment of gliomas rely mainly on surgical resection and the inhibition of tumour cell proliferation by irradiation and chemotherapy. Glioma stem cells are a subpopulation of proliferating tumour cells that have self-renewal capacity and can give rise to heterogeneous cells that comprise the tumour and are thought to play a role in the resistance of gliomas to therapy. Read More

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Electroacupuncture promotes the recovery of rats with spinal cord injury by suppressing the Notch signaling pathway via the H19/EZH2 axis.

Ann Transl Med 2021 May;9(10):844

Second Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China.

Background: Spinal cord injury (SCI) is a life-changing event with an extremely poor prognosis. In our preliminary studies, electroacupuncture (EA) was found to promote the repair of SCI, which was closely related to the Notch signaling pathway. Therefore, in the present study, we hypothesized that EA protects against SCI by inhibiting the Notch signaling pathway and sought to investigate the underlying molecular mechanisms. Read More

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Resveratrol-mediated neurorestoration after cerebral ischemic injury - Sonic Hedgehog signaling pathway.

Life Sci 2021 Sep 8;280:119715. Epub 2021 Jun 8.

Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

Aims: Resveratrol pretreatment can decrease ischemic cerebral injury and enhance proliferation of neural stem cells via mediation of Sonic Hedgehog signaling. However, it is relatively little known about whether neurorestorative effects of resveratrol are mediated by Shh signaling in ischemic cerebral injury. The present study tests whether the Shh signaling pathway mediates resveratrol to promote neurorestoration of ischemic cerebral injury. Read More

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September 2021

Angelica sinensis extract promotes neuronal survival by enhancing p38 MAPK-mediated hippocampal neurogenesis and dendritic growth in the chronic phase of transient global cerebral ischemia in rats.

J Ethnopharmacol 2021 Oct 4;278:114301. Epub 2021 Jun 4.

Department of Chinese Medicine, China Medical University Hospital, Taichung, 42056, Taiwan; Research Center for Chinese Medicine & Acupuncture, China Medical University, Taichung, 40402, Taiwan; Graduate Institute of Acupuncture Science, China Medical University, Taichung, 40402, Taiwan. Electronic address:

Ethnopharmacological Relevance: Angelica sinensis (Oliv.) Diels (ASD), commonly known as Dang Gui, is a popular Chinese herb that has long been used to treat ischemic stroke. However, the effects of ASD in chronic cerebral ischemia and its underlying mechanisms still remain unclear. Read More

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October 2021

The effect of melatonin on Hippo signaling pathway in dental pulp stem cells.

Neurochem Int 2021 Sep 25;148:105079. Epub 2021 May 25.

Department of Stem Cell Sciences, Graduate School of Health Sciences, Hacettepe University, Ankara, Turkey; Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, Ankara, Turkey. Electronic address:

Dental pulp stem cells (DPSCs) have a high capacity to differentiate into the neuronal cell lineage. Meanwhile, both Hippo signaling and melatonin are key regulators in neuronal differentiation of neuronal progenitor cells. Recently emerging evidences suggest the possible interaction between melatonin and Hippo signaling in different cell lines. Read More

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September 2021

Developmental neurotoxicity of antimony (Sb) in the early life stages of zebrafish.

Ecotoxicol Environ Saf 2021 May 8;218:112308. Epub 2021 May 8.

Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400030, China. Electronic address:

Accumulating studies have revealed the toxicity of antimony (Sb) to soil-dwelling and aquatic organisms at the individual level. However, little is known about the neurotoxic effects of antimony and its underlying mechanisms. To assess this issue, we investigated the neurotoxicity of antimony (0, 200, 400, 600 and 800 mg/L) in zebrafish embryos. Read More

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Transplantation of rat dental pulp stem cells facilities post-lesion recovery in the somatosensory whisker cortex of male Wistar rats.

Brain Res Bull 2021 Aug 5;173:150-161. Epub 2021 May 5.

Neuroscience Research Center, Institute of Neuropharmachology, Kerman University of Medical Sciences, Kerman, Iran; Cognitive Neuroscience Research Center, Institute of Neuropharmachology, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:

Damage to somatosensory "barrel" cortex reduces the rats' behavioral sensitivity in discrimination of tactile stimuli. Here, we examined how transplantation of stem cells into the lesioned barrel cortex can help in recovery of sensory capacities. We induced mechanical lesions in the right barrel cortex area of male rats. Read More

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Neural stem cells in the adult olfactory bulb core generate mature neurons in vivo.

Stem Cells 2021 May 8. Epub 2021 May 8.

Instituto Cajal-Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.

Although previous studies suggest that neural stem cells (NSCs) exist in the adult olfactory bulb (OB), their location, identity, and capacity to generate mature neurons in vivo has been little explored. Here, we injected enhanced green fluorescent protein (EGFP)-expressing retroviral particles into the OB core of adult mice to label dividing cells and to track the differentiation/maturation of any neurons they might generate. EGFP-labeled cells initially expressed adult NSC markers on days 1 to 3 postinjection (dpi), including Nestin, GLAST, Sox2, Prominin-1, and GFAP. Read More

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microRNA-125b and its downstream Smurf1/KLF2/ATF2 axis as important promoters on neurological function recovery in rats with spinal cord injury.

J Cell Mol Med 2021 May 5. Epub 2021 May 5.

Department of Spine Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.

The purpose of this study is to investigate the role of microRNA-125b (miR-125b) and its mechanism in spinal cord injury (SCI) by targeting Smurf1. After loss- and gain-function approaches were conducted in SCI rat models and neural stem cells (NSCs) isolated from foetal rats, the Basso-Beattie-Bresnahan (BBB) score was calculated, and related protein expression was determined by Western blot analysis and cell apoptosis by TUNEL staining. NSC viability was detected by CCK-8, migration abilities by Transwell assay and apoptosis by flow cytometry. Read More

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Amyloid-Beta Induces Different Expression Pattern of Tissue Transglutaminase and Its Isoforms on Olfactory Ensheathing Cells: Modulatory Effect of Indicaxanthin.

Int J Mol Sci 2021 Mar 25;22(7). Epub 2021 Mar 25.

Institute for Biomedical Research and Innovation (IRIB), National Research Council, 95126 Catania, Italy.

Herein, we assessed the effect of full native peptide of amyloid-beta (Aβ) (1-42) and its fragments (25-35 and 35-25) on tissue transglutaminase (TG2) and its isoforms (TG2-Long and TG2-Short) expression levels on olfactory ensheathing cells (OECs). Vimentin and glial fibrillary acid protein (GFAP) were also studied. The effect of the pre-treatment with indicaxanthin from Opuntia ficus-indica fruit on TG2 expression levels and its isoforms, cell viability, total reactive oxygen species (ROS), superoxide anion (O), and apoptotic pathway activation was assessed. Read More

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The Neural Stem Cell Properties of PKD2L1 Cerebrospinal Fluid-Contacting Neurons .

Front Cell Neurosci 2021 15;15:630882. Epub 2021 Mar 15.

Department of Orthopedics Traumatic, The Affiliated Hospital of Guizhou Medical University, Guiyang City, China.

Cerebrospinal fluid-touching neurons (CSF-cNs) exist in the region surrounding the central canal of the spinal cord, which locate in the adult neurogenic niche. Previous research showed that CSF-cNs expressed the molecular markers of immature neural cells . Here, we explored the potential of CSF-cNs as neural stem cell . Read More

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Microvesicles from Schwann-Like Cells as a New Biomaterial Promote Axonal Growth.

J Biomed Nanotechnol 2021 Feb;17(2):291-302

Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu, China.

Schwann cells promote axonal regeneration following peripheral nerve injury. However, in terms of clinical treatment, the therapeutic effects of Schwann cells are limited by their source. The transmission of microvesicles from neuroglia cells to axons is a novel communication mechanism in axon regeneration. Read More

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February 2021

Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury.

Sci China Life Sci 2021 Mar 24. Epub 2021 Mar 24.

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100190, China.

Nestin is expressed extensively in neural stem/progenitor cells during neural development, but its expression is mainly restricted to the ependymal cells in the adult spinal cord. After spinal cord injury (SCI), Nestin expression is reactivated and Nestin-positive (Nestin) cells aggregate at the injury site. However, the derivation of Nestin cells is not clearly defined. Read More

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SARM1 promotes neuroinflammation and inhibits neural regeneration after spinal cord injury through NF-κB signaling.

Theranostics 2021 20;11(9):4187-4206. Epub 2021 Feb 20.

Department of Orthopedics (Spine Surgery), The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.

Axonal degeneration is a common pathological feature in many acute and chronic neurological diseases such as spinal cord injury (SCI). SARM1 (sterile alpha and TIR motif-containing 1), the fifth TLR (Toll-like receptor) adaptor, has diverse functions in the immune and nervous systems, and recently has been identified as a key mediator of Wallerian degeneration (WD). However, the detailed functions of SARM1 after SCI still remain unclear. Read More

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February 2021

Saikosaponin-d improved the stemness of mouse neural stem cells and increased their thermotolerance potential.

Int J Dev Neurosci 2021 Jun 24;81(4):324-332. Epub 2021 Apr 24.

Department of Neonatology, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin, China.

Purpose: To investigate the effect of saikosaponin-d (Ssd) on proliferation, differentiation, and stemness of neural stem cells (NSCs), and to observe whether Ssd has a protective effect on NSCs at medium-high and high temperature.

Materials And Methods: NSCs were extracted from 15-day fetal mice. After subculture, Ssd treatment was performed. Read More

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miR-31 promotes neural stem cell proliferation and restores motor function after spinal cord injury.

Exp Biol Med (Maywood) 2021 Jun 9;246(11):1274-1286. Epub 2021 Mar 9.

Laboratory Animal Research Center of Shanxi Medical University, Shanxi Key Laboratory of Animal and Animal Model of Human Diseases, Taiyuan 030001, China.

This study aims to examine whether miR-31 promotes endogenous NSC proliferation and be used for spinal cord injury management. In the present study, the morpholino knockdown of miR-31 induced abnormal neuronal apoptosis in zebrafish, resulting in impaired development of the tail. miR-31 agomir transfection in NSCs increased Nestin expression and decreased ChAT and GFAP expression levels. Read More

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Exosomes derived from human placental mesenchymal stem cells enhanced the recovery of spinal cord injury by activating endogenous neurogenesis.

Stem Cell Res Ther 2021 03 12;12(1):174. Epub 2021 Mar 12.

Centre of Reproduction, Development and Aging, Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Room 4021, Building E12, Taipa, Macau, SAR, China.

Background: Spinal cord injury (SCI) is a debilitating medical condition that can result in the irreversible loss of sensorimotor function. Current therapies fail to provide an effective recovery being crucial to develop more effective approaches. Mesenchymal stem cell (MSC) exosomes have been shown to be able to facilitate axonal growth and act as mediators to regulate neurogenesis and neuroprotection, holding great therapeutic potential in SCI conditions. Read More

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Nrg1/ErbB2 regulates differentiation and apoptosis of neural stem cells in the cochlear nucleus through PI3K/Akt pathway.

Neurosci Lett 2021 04 8;751:135803. Epub 2021 Mar 8.

Departments of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi, PR China. Electronic address:

Sensorineural hearing loss (SNHL) is a common causes of disability. Neural stem cells (NSCs) from the cochlear nuclei have been considered to be a potential direction for the treatment of SNHL. Neuregulin 1 (NRG1)/ErbB2 signaling displays an essential role in nervous system development. Read More

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Small Molecule Treatments Improve Differentiation Potential of Human Amniotic Fluid Stem Cells.

Front Bioeng Biotechnol 2021 22;9:623886. Epub 2021 Feb 22.

Department of Molecular Cell Biology, Life Sciences Center, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.

Human amniotic fluid stem cells (AFSC) are an exciting and very promising source of stem cells for therapeutic applications. In this study we investigated the effects of short-term treatments of small molecules to improve stem cell properties and differentiation capability. For this purpose, we used epigenetically active compounds, such as histone deacetylase inhibitors Trichostatin A (TSA) and sodium butyrate (NaBut), as well as multifunctional molecules of natural origin, such as retinoic acid (RA) and vitamin C (vitC). Read More

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February 2021

Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑trans retinoic acid.

Mol Med Rep 2021 03 21;23(3). Epub 2021 Jan 21.

Department of Pediatrics, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, P.R. China.

Neural tube defects (NTDs) are the most serious and common birth defects in the clinical setting. The Notch signaling pathway has been implicated in different processes of the embryonic neural stem cells (NSCs) during neural tube development. The aim of the present study was to investigate the expression pattern and function of Notch1 (N1) in all‑trans retinoic acid (atRA)‑induced NTDs and NSC differentiation. Read More

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In vitro investigation of growth factors including MGF and IGF-1 in neural stem cell activation, proliferation, and migration.

Brain Res 2021 May 16;1759:147366. Epub 2021 Feb 16.

Department of Genetics, Aziz Sancar Research Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. Electronic address:

Neurogenesis is mainly activated after damage in adult tissues. This destruction activates the neural stem cells (NSCs) by exiting from a quiescent state and initiating proliferation, differentiation, and migration towards the damaged area. Although studies have investigated to clarify the process of NSC biology and neurogenesis, there are still significant artifacts in understanding the primary mechanism. Read More

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FOXO3 Regulates Sevoflurane-Induced Neural Stem Cell Differentiation in Fetal Rats.

Cell Mol Neurobiol 2021 Feb 19. Epub 2021 Feb 19.

Department of Anesthesiology, Shengjing Hospital of China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, 110004, Liaoning, China.

With the increase in fetal surgeries, the effect of maternal anesthesia on progeny has attracted much attention. Our previous studies have demonstrated that 3.5% sevoflurane maternal exposure resulted in over-activated autophagy and cognitive impairment in the offspring. Read More

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February 2021

Preemptive Stem Cells Ameliorate Neuropathic Pain in Rats: A Central Component of Preemptive Analgesia.

Microsc Microanal 2021 04;27(2):450-456

Department of pharmacology, Faculty of Medicine, Assiut University, Asyut, Egypt.

The present study aims to investigate the efficacy of intravenously injected mesenchymal stem cells (MSCs) in treating neuropathic pain either before or after its induction by a chronic constriction injury (CCI) model. Rats were divided into four groups: control group, neuropathic group, and treated groups (pre and postinduction) with i.v. Read More

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Dental Pulp From Human Exfoliated Deciduous Teeth-Derived Stromal Cells With Demonstrated Neuronal Potential: In Vivo And In Vitro Studies.

Curr Stem Cell Res Ther 2021 Feb 15. Epub 2021 Feb 15.

Core for Cell Technology, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba. Brazil.

Background: Mesenchymal stromal cells (MSC) have the potential for self-renewal and differentiation in different tissues, characteristics that encourage their use in regenerative medicine. Dental tissue MSCs are easy to collect, have the same embryonic origin as neurons and have neuronal markers that allow their use in treating neurodegenerative diseases. Human exfoliated deciduous teeth (SHED)-derived stromal cells are considered immature and present positive expression of pluripotency and neuronal markers. Read More

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February 2021

Prenatal ethanol exposure impairs the formation of radial glial fibers and promotes the transformation of GFAPδ‑positive radial glial cells into astrocytes.

Mol Med Rep 2021 04 12;23(4). Epub 2021 Feb 12.

Shaanxi Center for Models of Clinical Medicine in International Cooperation of Science and Technology, Shaanxi Provincial People's Hospital and The Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710068, P.R. China.

During embryonic cortical development, radial glial cells (RGCs) are the major source of neurons, and these also serve as a supportive scaffold to guide neuronal migration. Similar to Vimentin, glial fibrillary acidic protein (GFAP) is one of the major intermediate filament proteins present in glial cells. Previous studies confirmed that prenatal ethanol exposure (PEE) significantly affected the levels of GFAP and increased the disassembly of radial glial fibers. Read More

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Isolation and characterization of cancer stem cells derived from human glioblastoma.

Am J Cancer Res 2021 1;11(2):441-457. Epub 2021 Feb 1.

Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Okayama 700-8530, Japan.

Cancer stem cell (CSC) is considered as a cause of cancer recurrence and metastasis. Simultaneously CSCs are responsible for the heterogeneous population in tumor tissues due to their differentiation potential. However, the characterizations of CSCs are still not enough and cancer stem cell lines widely available is desired to be established for the advancement of cancer research. Read More

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February 2021

In vitro characterization of subventricular zone isolated neural stem cells, from adult monkey and rat brain.

Mol Biol Rep 2021 Feb 10;48(2):1311-1321. Epub 2021 Feb 10.

Department of Stem Cell and Developmental Biology, Cell Science Research Center, ROYAN Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.

Neural stem cells (NSCs) are multipotent, self-renewable cells who are capable of differentiating into neurons, astrocytes, and oligodendrocytes. NSCs reside at the subventricular zone (SVZ) of the adult brain permanently to guarantee a lifelong neurogenesis during neural network plasticity or undesirable injuries. Although the specious inaccessibility of adult NSCs niche hampers their in vivo identification, researchers have been seeking ways to optimize adult NSCs isolation, expansion, and differentiation, in vitro. Read More

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February 2021