209,995 results match your criteria germ cell


Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line.

Stem Cell Res 2021 Sep 11;56:102536. Epub 2021 Sep 11.

Department of Biology, University of Louisiana at Lafayette, 410 East Saint Mary Boulevard, Lafayette LA 70503, USA. Electronic address:

A typical DYT1 dystonia is caused by a heterozygous GAG deletion (c.907-909) in the TOR1A gene (ΔE, p.Glu303del) and the pathogenesis is not clear. Read More

View Article and Full-Text PDF
September 2021

Eya1 protein distribution during embryonic development of Xenopus laevis.

Gene Expr Patterns 2021 Sep 15:119213. Epub 2021 Sep 15.

School of Natural Sciences, National University of Galway, Galway, Ireland. Electronic address:

Eya1 and other Eya proteins are important regulators of progenitor proliferation, cell differentiation and morphogenesis in all three germ layers. At present, most of our knowledge of Eya1 distribution is based on in situ hybridization for Eya1 mRNA. However, to begin to dissect the mechanisms underlying Eya1 functions, we need a better understanding of the spatiotemporal distribution of Eya1 proteins during embryonic development, their subcellular localization and their levels of expression in various tissues. Read More

View Article and Full-Text PDF
September 2021

The mutagenic effect of tobacco smoke on male fertility.

Environ Sci Pollut Res Int 2021 Sep 18. Epub 2021 Sep 18.

College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates.

Despite the association between tobacco use and the harmful effects on general health as well as male fertility parameters, smoking remains globally prevalent. The main content of tobacco smoke is nicotine and its metabolite cotinine. These compounds can pass the blood-testis barrier, which subsequently causes harm of diverse degree to the germ cells. Read More

View Article and Full-Text PDF
September 2021

Yarrowia lipolytica, health benefits for animals.

Appl Microbiol Biotechnol 2021 Sep 18. Epub 2021 Sep 18.

Immunology & Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste, SC., Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz, B.C.S. C.P., 23096, México.

The yeast Yarrowia lipolytica has been industrially adopted for docosahexaenoic acid and eicosapentaenoic acid production under good manufacturing practices over 2 decades. In recent years, it has claimed attention for novel biotechnological applications, such as a functional feed additive for animals. Studies have demonstrated that this yeast is safe and has probiotic and nutritional properties for mammals, birds, fish, crustaceans, and molluscs. Read More

View Article and Full-Text PDF
September 2021

Ignored Papers, Invented Quotations: A History of Fetal Alcohol Syndrome.

Authors:
Michael Obladen

Neonatology 2021 Sep 14:1-7. Epub 2021 Sep 14.

Department of Neonatology, Charité University Medicine, Berlin, Germany.

Given the high rate of alcoholism throughout history, its effects on the fetus may have existed for millennia. But, the claim that Greeks and Romans were aware of fetal alcohol syndrome rests on incorrect citations. From 1725, maternal alcohol consumption was associated with retarded fetal growth and neurological anomalies. Read More

View Article and Full-Text PDF
September 2021

The emerging role of mitochondrial derived peptide humanin in the testis.

Biochim Biophys Acta Gen Subj 2021 Sep 14;1865(12):130009. Epub 2021 Sep 14.

Division of Endocrinology, Department of Medicine, The Lundquist Institute and Harbor-UCLA Medical Center, Torrance, CA, United States of America. Electronic address:

The discovery of mitochondrial derive peptides (MDPs) has spotlighted mitochondria as central hubs in control and regulation of cell viability and metabolism in the testis in response to intracellular and extracellular stresses. MDPs (Humanin, MOTS-c and SHLP-2) are present in testes. Humanin, the first MDP, is predominantly expressed in Leydig cells, and moderately in germ cells and seminal plasma. Read More

View Article and Full-Text PDF
September 2021

Conditional immobilization for live imaging Caenorhabditis elegans using auxin-dependent protein depletion.

G3 (Bethesda) 2021 Sep 2. Epub 2021 Sep 2.

Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USA.

The visualization of biological processes using fluorescent proteins and dyes in living organisms has enabled numerous scientific discoveries. The nematode Caenorhabditis elegans is a widely used model organism for live imaging studies since the transparent nature of the worm enables imaging of nearly all tissues within a whole, intact animal. While current techniques are optimized to enable the immobilization of hermaphrodite worms for live imaging, many of these approaches fail to successfully restrain the smaller male worms. Read More

View Article and Full-Text PDF
September 2021

PiRNA pathway in the cardiovascular system: a novel regulator of cardiac differentiation, repair and regeneration.

J Mol Med (Berl) 2021 Sep 17. Epub 2021 Sep 17.

Xiamen Key Laboratory of Cardiovascular Disease, Xiamen Cardiovascular Hospital Xiamen University, Xiamen, China.

Piwi-interacting RNAs (piRNAs) are a novel group of small non-coding RNA molecules with lengths of 21-35 nucleotides, first identified from the germline. PiRNAs and their associated PIWI clade Argonaute proteins constitute a key part of the piRNA pathway, with the best-known biological function to silence transposable elements in germ cells. The piRNA pathway, in fact, is not exclusive to the germline. Read More

View Article and Full-Text PDF
September 2021

Biology of primordial germ cells in vertebrates with emphasis in urodeles amphibians.

Mol Reprod Dev 2021 Sep 17. Epub 2021 Sep 17.

Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México, México.

Primordial germ cells (PGCs) are highly specialized cells that play a relevant role in the maintenance and evolution of the species, since they create new combinations of genetic information between the organisms. Amphibians are a class of amniote vertebrates that are divided into three subclasses, the anurans (frogs and toads), the urodeles (salamanders and newts), and the gymnophiones (caecilians). The study of PGCs in amphibians has been addressed in more detail in anurans while little is known about the biology of this cell lineage in urodeles. Read More

View Article and Full-Text PDF
September 2021

Late Testicular Yolk Sac Tumor With Cerebellar Metastasis and a Rapid Recurrence After a Gross Total Resection: A Case Report.

Cureus 2021 Aug 13;13(8):e17143. Epub 2021 Aug 13.

Department of Neurosurgery, University of Texas Medical Branch, Galveston, USA.

Yolk sac tumor (YST) is the most common prepubertal testicular tumor. It is considered a subtype of non-seminoma germ cell tumor (NSGCT) that is presumed to have an aggressive behavior with high malignant potential, thus requiring multimodality treatment with resection and chemotherapy. Treatment is curative for the majority of patients, even the ones with relapse after a few years. Read More

View Article and Full-Text PDF

Pan-Cancer Analysis of PARP1 Alterations as Biomarkers in the Prediction of Immunotherapeutic Effects and the Association of Its Expression Levels and Immunotherapy Signatures.

Front Immunol 2021 31;12:721030. Epub 2021 Aug 31.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Background: Poly (ADP-ribose) polymerases-1 (PARP1) alterations are associated with PARP1 inhibitor resistance, regulating the function of Treg cells and PDL1 expression in tumor cells, and high PARP1 expression is significantly associated with aggressive behavior and chemotherapeutic resistance in several tumors. However, a comprehensive analysis of the predictive values of PARP1 alteration for immune checkpoint inhibitor (ICI) effectiveness in tumors remains unclear, and the associations between its expression and immunotherapy signatures also needs to be explored further.

Methods: We performed some analyses with the cBioPortal online database (https://www. Read More

View Article and Full-Text PDF

Hecw controls oogenesis and neuronal homeostasis by promoting the liquid state of ribonucleoprotein particles.

Nat Commun 2021 Sep 16;12(1):5488. Epub 2021 Sep 16.

IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan, Italy.

Specialised ribonucleoprotein (RNP) granules are a hallmark of polarized cells, like neurons and germ cells. Among their main functions is the spatial and temporal modulation of the activity of specific mRNA transcripts that allow specification of primary embryonic axes. While RNPs composition and role are well established, their regulation is poorly defined. Read More

View Article and Full-Text PDF
September 2021

YAP creates epiblast responsiveness to inductive signals for germ cell fate.

Development 2021 Sep 16. Epub 2021 Sep 16.

Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster, Germany.

The germ cell lineage in mammals is induced by the stimulation of pluripotent epiblast cells with signaling molecules. Previous studies have suggested that the germ cell differentiation competence or responsiveness of epiblast cells to signaling molecules is established and maintained in epiblast cells of a specific differentiation state. However, the molecular mechanism underlying this process has not been well defined. Read More

View Article and Full-Text PDF
September 2021

Contrast-enhanced ultrasound as a valuable imaging modality for characterizing testicular lesions.

Asian J Androl 2021 Sep 14. Epub 2021 Sep 14.

Department of Ultrasound, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China.

Contrast-enhanced ultrasound (CEUS) is a new form of ultrasound (US) that can dynamically display microvessels in a highly sensitive manner. The purpose of this study was to investigate the efficacy of CEUS for characterizing testicular lesions in comparison with conventional US. Forty-seven patients with testicular lesions were enrolled. Read More

View Article and Full-Text PDF
September 2021

The interrelationship between female reproductive aging and survival.

J Gerontol A Biol Sci Med Sci 2021 Sep 16. Epub 2021 Sep 16.

Department of Animal, Dairy and Veterinary Sciences, Center for Integrated BioSystems, School of Veterinary Medicine, Utah State University, 4700 Old Main Hill, Logan, UT 84322, USA.

The link between survival and reproductive function is demonstrated across many species and is under both long-term evolutionary pressures and short-term environmental pressures. Loss of reproductive function is common in mammals and is strongly correlated with increased rates of disease in both males and females. However, the reproduction-associated change in disease rates is more abrupt and more severe in women, who benefit from a significant health advantage over men until the age of menopause. Read More

View Article and Full-Text PDF
September 2021

Detection of homolog-independent meiotic DNA repair events in with the intersister/intrachromatid repair assay.

STAR Protoc 2021 Sep 8;2(3):100801. Epub 2021 Sep 8.

Institute of Molecular Biology, Department of Biology, University of Oregon, 1229 Franklin Boulevard, Eugene, OR 97403, USA.

Accurate repair of DNA double-strand breaks (DSBs) in developing germ cells is critical to promote proper chromosome segregation and to maintain genome integrity. To directly detect homolog-independent (intersister/intrachromatid) meiotic DSB repair, we exploited the genetics and germline physiology of to (1) induce a single DSB in nuclei across discrete stages of meiotic prophase I; (2) detect repair of that DSB as a homolog-independent crossover or noncrossover; and (3) sequence the resultant product to assess mechanisms of recombination. For complete details on the use and execution of this protocol, please refer to Toraason et al. Read More

View Article and Full-Text PDF
September 2021

Prompt diagnosis and management of a ruptured mediastinal cystic teratoma.

Radiol Case Rep 2021 Nov 6;16(11):3461-3464. Epub 2021 Sep 6.

Department of Radiology, Kirk Kerkorian School of Medicine at UNLV, University of Nevada Las Vegas School of Medicine, 1001 Shadow Ln, Las Vegas, NV 89106, USA.

Mediastinal germ cell tumors are some of the less frequently encountered anterior mediastinal masses. We report an interesting case of a 26-year-old male with a ruptured mediastinal cystic teratoma. Initial plain radiograph and CT scan of the chest showed radiographic evidence of a ruptured cystic teratoma, including a peripherally enhancing, partially calcified mass with internal fat density. Read More

View Article and Full-Text PDF
November 2021

Integrative pan-cancer analysis of MEK1 aberrations and the potential clinical implications.

Sci Rep 2021 Sep 15;11(1):18366. Epub 2021 Sep 15.

Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Alterations of mitogen-activated protein kinase kinase 1 (MEK1) are commonly associated with tumorigenesis, and MEK1 is thought to be a suitable targeted therapy for various cancers. However, abnormal MEK1 alterations and their relevant clinical implications are unknown. Our research comprehensively analyzed the MEK1 alteration spectrum and provided novel insight for targeted therapies. Read More

View Article and Full-Text PDF
September 2021

Male cancer patient sperm cryopreservation for fertility preservation: 10-year monocentric experience.

Basic Clin Androl 2021 Sep 16;31(1):24. Epub 2021 Sep 16.

Human Sperm Bank, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Ministry of Education, Sichuan University, 610041, Sichuan, P.R. China.

Background: Sperm cryopreservation, an effective method for preserving male fertility, is very advantageous for men suffering from cancer. Unfortunately, as both physicians and cancer patients are unaware of the possibilities for sperm cryopreservation, the data on evaluation of semen parameters and disposition of cryopreserved samples among Chinese cancer patients are scarce.

Results: Male tumours were classified into six major types, germ cell tumours (26 %), haematological neoplasms (28 %), head and neck cancers (19 %), thoracic tumours (4 %), abdominal tumours (10 %), and others (13 %). Read More

View Article and Full-Text PDF
September 2021

IL-1 Mediates Microbiome-Induced Inflamm-Ageing of Hematopoietic Stem Cells in Mice.

Blood 2021 Sep 15. Epub 2021 Sep 15.

University Hospital Zurich and University of Zurich, Zurich, Switzerland.

Ageing is associated with impaired hematopoietic and immune function. This is caused in part by decreased hematopoietic stem cell (HSC) population fitness and an increased myeloid differentiation bias. The reasons for this aging-associated HSC impairment are incompletely understood. Read More

View Article and Full-Text PDF
September 2021

Robotic Surgery in Pediatric Oncology: Lessons Learned from the First 100 Tumors-A Nationwide Experience.

Ann Surg Oncol 2021 Sep 14. Epub 2021 Sep 14.

Department of Pediatric Surgery and Urology, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France.

Background: While robotics has become commonplace in adult oncology, it remains rare in pediatric oncology due to the rarity of childhood cancers. We present the results of a large nationwide experience with robotic oncology, with the aim of providing practical and feasible guidelines for child selection.

Methods: This was a prospective analysis performed over a period of 4 years. Read More

View Article and Full-Text PDF
September 2021

An update on oral drug delivery intestinal lymphatic transport.

Acta Pharm Sin B 2021 Aug 9;11(8):2449-2468. Epub 2021 Apr 9.

Key Laboratory of Smart Drug Delivery of MOE, School of Pharmacy, Fudan University, Shanghai 201203, China.

Orally administered drug entities have to survive the harsh gastrointestinal environment, penetrate the enteric epithelia and circumvent hepatic metabolism before reaching the systemic circulation. Whereas the gastrointestinal stability can be well maintained by taking proper measures, hepatic metabolism presents as a formidable barrier to drugs suffering from first-pass metabolism. The pharmaceutical academia and industries are seeking alternative pathways for drug transport to circumvent problems associated with the portal pathway. Read More

View Article and Full-Text PDF

Oral delivery of proteins and peptides: Challenges, status quo and future perspectives.

Acta Pharm Sin B 2021 Aug 29;11(8):2416-2448. Epub 2021 Apr 29.

Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443, China.

Proteins and peptides (PPs) have gradually become more attractive therapeutic molecules than small molecular drugs due to their high selectivity and efficacy, but fewer side effects. Owing to the poor stability and limited permeability through gastrointestinal (GI) tract and epithelia, the therapeutic PPs are usually administered by parenteral route. Given the big demand for oral administration in clinical use, a variety of researches focused on developing new technologies to overcome GI barriers of PPs, such as enteric coating, enzyme inhibitors, permeation enhancers, nanoparticles, as well as intestinal microdevices. Read More

View Article and Full-Text PDF

Expression and localization of Sox10 during hair follicle morphogenesis and induced hair cycle.

Int J Med Sci 2021 13;18(15):3498-3505. Epub 2021 Aug 13.

Department of Dermatology, the second affiliated hospital of Zhejiang University, Hangzhou 310000, China.

Sox transcription factors play many diverse roles during development, including regulating stem cell states, directing differentiation, and influencing the local chromatin landscape. Sox10 has been implicated in the control of stem/progenitor activity and epithelial-mesenchymal transition, yet it has not been studied in relation to the hair follicle cycle or hair follicle stem cell (HFSC) control. To elucidate the role of Sox10 in hair follicle cycle control, we performed immunohistochemical and immunofluorescence analysis of its expression during hair morphogenesis, the postnatal hair cycle, and the depilation-induced murine hair follicle cycle. Read More

View Article and Full-Text PDF

[Association between Immunotherapy with Immune Checkpoint Inhibitors(Anti-PD-1 Antibodies)and Intestinal Microbiota].

Gan To Kagaku Ryoho 2021 Sep;48(9):1096-1099

Dept. of Chest Surgery, Fukushima Medical University.

Immune checkpoint inhibitors(ICIs)are playing an increasingly important role in the treatment of cancer. In the field of lung cancer, ICIs are widely administered from primary therapy to maintenance therapy after chemoradiation for non-small cell lung cancer. However, excluding tumor proportion score(TPS)for PD-L1, no other biomarker has been reported to be clinically useful. Read More

View Article and Full-Text PDF
September 2021

Vinblastine monotherapy induction prior to radiotherapy for patients with intracranial germinoma during the COVID-19 pandemic.

Pediatr Blood Cancer 2021 Sep 14:e29359. Epub 2021 Sep 14.

Department of Paediatric Haematology and Oncology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK.

Background: Patients with localized intracranial germinoma have excellent survival. Reducing treatment burden and long-term sequelae is a priority. Intensive inpatient chemotherapy (e. Read More

View Article and Full-Text PDF
September 2021

Protective effects of Lepidium draba L. leaves extract on testis histopathology, oxidative stress indicators, serum reproductive hormones and inflammatory signalling in oxymetholone-treated rat.

Andrologia 2021 Sep 14:e14239. Epub 2021 Sep 14.

College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China.

This study was aimed to investigate the protective effects of Lepidium draba L. (L. draba) extract on oxymetholone (OM)-induced testicular injury in rat. Read More

View Article and Full-Text PDF
September 2021

The therapeutic potential of adipose tissue-derived mesenchymal stromal cells in the treatment of busulfan-induced azoospermic mice.

J Assist Reprod Genet 2021 Sep 14. Epub 2021 Sep 14.

Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Purpose: The generation of germ cells from mesenchymal stromal cells (MSCs) provides a valuable in vitro platform for infertility modeling. The establishment of these cells is a new approach for assisted reproductive technology (ART) to help infertile patients who lack functional gametes.

Methods: Human adipose-derived MSCs were isolated and then characterized for multipotency by flow cytometry, differentiation capacity, and cytogenetic assays. Read More

View Article and Full-Text PDF
September 2021

The zinc-finger protein OEF-1 stabilizes histone modification patterns and promotes efficient splicing in the C. elegans germ line.

G3 (Bethesda) 2021 Sep 14. Epub 2021 Sep 14.

Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.

To ensure stable transmission of genetic information to the next generation, germ cells frequently silence sex chromosomes, as well as autosomal loci that promote inappropriate differentiation programs. In C. elegans, silenced and active genomic domains are established in germ cells by the histone modification complexes MES-2/3/6 and MES-4, which promote silent and active chromatin states, respectively. Read More

View Article and Full-Text PDF
September 2021

A fungal powdery mildew pathogen induces extensive local and marginal systemic changes in the Arabidopsis thaliana microbiota.

Environ Microbiol 2021 Sep 14. Epub 2021 Sep 14.

RWTH Aachen University, Institute for Biology I, Unit of Plant Molecular Cell Biology, Worringerweg 1, 52056, Aachen, Germany.

Powdery mildew is a foliar disease caused by epiphytically growing obligate biotrophic ascomycete fungi. How powdery mildew colonization affects host resident microbial communities locally and systemically remains poorly explored. We performed powdery mildew (Golovinomyces orontii) infection experiments with Arabidopsis thaliana grown in either natural soil or a gnotobiotic system and studied the influence of pathogen invasion into standing natural multi-kingdom or synthetic bacterial communities (SynComs). Read More

View Article and Full-Text PDF
September 2021