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Taurine Upregulates miRNA-122-5p Expression and Suppresses the Metabolizing Enzymes of Glycolytic Pathway in Hepatocellular Carcinoma.

Mol Biol Rep 2021 Jul 27. Epub 2021 Jul 27.

Department of Zoology, Faculty of Science, Cairo University, Giza, 12613, Egypt.

Background: Hepatocellular carcinoma (HCC) is a complicated disease with a poor prognosis and high mortality rates. The prevention, control, diagnosis, and treatment of liver cancer have become vital focuses in healthcare research.

Aim: This study aimed to evaluate the in vitro effect of taurine (Tau) on the expression of miR-122-5p that targets some limiting glycolytic enzymes and affects the overall glycolytic pathway in HepG2 cells. Read More

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A putative redox-sensing regulator Rex regulates lincomycin biosynthesis in Streptomyces lincolnensis.

J Basic Microbiol 2021 Jul 27. Epub 2021 Jul 27.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.

Lincomycin is an important antimicrobial agent which is widely used in clinical and animal husbandry. The biosynthetic pathway of lincomycin comes to light in the past 10 years, however, the regulatory mechanism is still unclear. In this study, a redox-sensing regulator Rex from Streptomyces lincolnensis (Rex ) was identified and characterized to affect cell growth and lincomycin biosynthesis. Read More

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Effect of protopine exposure on the physiology and gene expression in the bloom-forming cyanobacterium Microcystis aeruginosa.

Environ Sci Pollut Res Int 2021 Jul 27. Epub 2021 Jul 27.

Geographical Science College, Taiyuan Normal University, Jinzhong, 030619, China.

Environment-friendly sound measures with high algal growth inhibition efficiency are required to control and eliminate CyanoHABs. This study examined the effects of protopine on growth, gene expression, and antioxidant system of the M. aeruginosa TY001 and explored possible damage mechanism. Read More

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Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2.

Virus Genes 2021 Jul 26. Epub 2021 Jul 26.

China Animal Health and Epidemiology Center, Qingdao, Shandong, China.

In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using other duck pathogens. The detection limit was 20 copies per µL. Read More

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The enhanced phosphorus use efficiency in phosphate-deficient and mycorrhiza-inoculated barley seedlings involves activation of different sets of PHT1 transporters in roots.

Planta 2021 Jul 27;254(2):38. Epub 2021 Jul 27.

Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, Telangana, 500046, India.

Main Conclusion: Transcriptional activation of subfamily II PHT1 members in roots is associated with the enhanced phosphorus use efficiency and growth promotion of barley seedlings inoculated with Glomus species. The arbuscular mycorrhizal (AM) fungi symbiotic associations in cereal crops are known to regulate growth in cultivar-specific manner and induce phosphate (Pi) transporters (PHT1) in roots. In the present study, we observed that both AM colonization of roots by Glomus species and phosphate starvation enhanced phosphorus use efficiency (PUE) in barley seedlings. Read More

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STRONG: metagenomics strain resolution on assembly graphs.

Genome Biol 2021 Jul 26;22(1):214. Epub 2021 Jul 26.

The iThree institute, University of Technology Sydney, 15 Broadway, Ultimo, 2007, NSW, Australia.

We introduce STrain Resolution ON assembly Graphs (STRONG), which identifies strains de novo, from multiple metagenome samples. STRONG performs coassembly, and binning into metagenome assembled genomes (MAGs), and stores the coassembly graph prior to variant simplification. This enables the subgraphs and their unitig per-sample coverages, for individual single-copy core genes (SCGs) in each MAG, to be extracted. Read More

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Drug resistance evaluation in novel 3D in vitro model.

Biomed Pharmacother 2021 Jun 31;138:111536. Epub 2021 Mar 31.

Institute of Health Sciences, Collegium Medicum, University of Zielona Gora, Zyty 28 St, 65-046 Zielona Gora, Poland. Electronic address:

Ovarian cancer rates the highest mortality among all gynecological malignancies. The main reason for high mortality is the development of drug resistance. It can be related to changes in the expression of many drug resistance genes as well as expression of extracellular matrix proteins and cell density in the tumor. Read More

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Phenotypic and Genotypic Characteristics of Uropathogenic Isolates from Kenya.

Microb Drug Resist 2021 Jul 23. Epub 2021 Jul 23.

Department of Emerging Infectious Diseases, United States Army Medical Research Directorate-Africa, Kenya/Kenya Medical Research Institute, Nairobi, Kenya.

Uropathogenic (UPECs) are a significant cause of urinary tract infections (UTIs). In Kenya, UTIs are typically treated with β-lactam antibiotics without antibiotic susceptibility testing, which could accelerate antibiotic resistance among UPEC strains. This study determined the occurrence of UPEC producing extended-spectrum β-lactamases (ESBLs), the genes conferring resistance to β-lactams, and the phylogenetic groups associated with ESBLs in Kenyan UPECs. Read More

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Propolis Can Improve Caudal Fin Regeneration in Zebrafish () Induced by The Combined Administration of Alloxan and Glucose.

Zebrafish 2021 Jul 23. Epub 2021 Jul 23.

Parasitology Division, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.

Hyperglycemia, a primary symptom in diabetes mellitus, is associated with difficulties in wound healing and regeneration. This condition is due to the length of the inflammatory phase and free radicals. Furthermore, there is evidence that molecular pathogenesis is involved in impaired wound healing in diabetics. Read More

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Exploring novel capping framework: high substituent pyridine-hydroxamic acid derivatives as potential antiproliferative agents.

Daru 2021 Jul 23. Epub 2021 Jul 23.

Departamento de Química, Universidad de Guanajuato, Guanajuato, Gto, 36050, México.

Purpose: Histone deacetylases (HDACs) play a vital role in the epigenetic regulation of gene expression due to their overexpression in several cancer forms. Therefore, these enzymes are considered as a potential anticancer drug target. Different synthetic and natural structures have been studied as HDACs inhibitors; based on available structural design information, the capping group is important for the biological activity due to the different interactions in the active site entrance. Read More

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Comparative analyses of the venom components in the salivary gland transcriptomes and saliva proteomes of some heteropteran insects.

Insect Sci 2021 Jul 23. Epub 2021 Jul 23.

Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Korea.

Salivary gland-specific transcriptomes of nine heteropteran insects with distinct feeding strategies (predaceous, hematophagous, and phytophagous) were analyzed and annotated to compare and identify the venom components as well as their expression profiles. The transcriptional abundance of venom genes was verified via quantitative real-time PCR. Hierarchical clustering of 30 representative differentially expressed venom genes from the nine heteropteran species revealed unique groups of salivary gland-specific genes depending on their feeding strategy. Read More

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N6-Methyladenosine RNA modification in cerebrospinal fluid as a novel potential diagnostic biomarker for progressive multiple sclerosis.

J Transl Med 2021 Jul 22;19(1):316. Epub 2021 Jul 22.

Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.

Background: Progressive multiple sclerosis (PMS) is an uncommon and severe subtype of MS that worsens gradually and leads to irreversible disabilities in young adults. Currently, there are no applicable or reliable biomarkers to distinguish PMS from relapsing-remitting multiple sclerosis (RRMS). Previous studies have demonstrated that dysfunction of N6-methyladenosine (m6A) RNA modification is relevant to many neurological disorders. Read More

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IκB kinase-β regulates neutrophil recruitment through activation of STAT3 signaling in the esophagus.

Cell Mol Gastroenterol Hepatol 2021 Jul 23. Epub 2021 Jul 23.

Department of Medicine, Gastroenterology and Hepatology Division, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. Electronic address:

Background & Aims: The epithelial barrier is the host's first line of defense against damage to the underlying tissue. Upon injury, the epithelium plays a critical role in inflammation. The IKKβ/NF-κB pathway was shown to be active in the esophageal epithelium of patients with esophageal disease. Read More

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A fast HRMA tool to authenticate eight salmonid species in commercial food products.

Food Chem Toxicol 2021 Jul 23:112440. Epub 2021 Jul 23.

LAQV-REQUIMTE, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313, Porto, Portugal. Electronic address:

Atlantic and Pacific salmon are frequently consumed species with very different economic values: farmed Atlantic salmon is cheaper than wild-caught Pacific salmons. Species replacements occur with the high valued Pacific species (Oncorhynchus keta, O. gorbuscha, O. Read More

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Cluster Regulates Primordial Germ Cells Differentiation from Human Mesenchymal Stem Cells.

Cell J 2021 Aug 17;23(3):294-302. Epub 2021 Jul 17.

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Objective: Numerous evidence indicates that microRNAs (miRNAs) are critical regulators in the spermatogenesis process. The aim of this study was to investigate cluster regulates primordial germ cells (PGCs) differentiation from human mesenchymal stem cells (MSCs).

Materials And Methods: In this experimental study, samples containing male adipose (n: 9 samples- age: 25-40 years) were obtained from cosmetic surgeries performed for the liposuction in Imam Khomeini Hospital. Read More

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LC89 exerts antidiabetic effects through regulating hepatic glucagon response and gut microbiota in type 2 diabetic mice.

Food Funct 2021 Jul 26. Epub 2021 Jul 26.

Key Laboratory of Food Nutrition and Safety, Ministry of Education & Tianjin Key Laboratory of Food Nutrition and Safety, Food Biotechnology Engineering Research Center of Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China. and Tianjin Agricultural University, Tianjin 300384, China.

Previous study suggests that Lactobacillus casei exhibits antihyperglycemic activity, however, the molecular mechanism of this has yet to be elucidated. Here, the anti-diabetic effects and underlying mechanisms of Lactobacillus casei LC89 are investigated in type 2 diabetes mellitus (T2DM) mice, which was induced by a high-fat diet (HFD) with streptozotocin (100 mg per kg BW). The results show that LC89 at a dose of 109 CFU day-1 decreases fasting blood glucose (FBG) and insulin levels by 35. Read More

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The roles of different strains in protecting against DSS-induced ulcerative colitis and related functional genes.

Food Funct 2021 Jul 26. Epub 2021 Jul 26.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China. and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.

The role of supplementation with different Bacteroides fragilis (B. fragilis) strains in alleviating ulcerative colitis (UC) is unclear due to the controversial results from animal experiments. In this study, three B. Read More

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Exosomes of Mesenchymal Stem Cells as a Proper Vehicle for Transfecting miR-145 into the Breast Cancer Cell Line and Its Effect on Metastasis.

Biomed Res Int 2021 29;2021:5516078. Epub 2021 Jun 29.

Department of Mesenchymal Stem Cells, Academic Center for Education, Culture and Research (ACECR), Qom Branch, Qom, Iran.

Background: Despite recent advances in scientific knowledge and clinical practice, management, and treatment of breast cancer, as one of the leading causes of female mortality, breast cancer remains a major burden. Recently, methods employing stem cells and their derivatives, i.e. Read More

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Development and Validation of a Ferroptosis-Related Gene Signature for Overall Survival Prediction in Lung Adenocarcinoma.

Front Cell Dev Biol 2021 7;9:684259. Epub 2021 Jul 7.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Ferroptosis is an iron-dependent programmed cell death process. Recent studies have found that ferroptosis inducers hold promising potential in the treatment of lung adenocarcinoma (LUAD). However, the comprehensive analysis about the prognostic value of ferroptosis-related genes in LUAD remains to be elucidated. Read More

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High-fat diet promotes epithelial-mesenchymal transition through enlarged growth of opportunistic pathogens and the intervention of saturated hydrogen.

Am J Transl Res 2021 15;13(6):6016-6030. Epub 2021 Jun 15.

Department of Medical Microbiology, Xiangya School of Medicine, Central South University Changsha, China.

Objectives: This study investigated the effects and mechanism of high-fat diet on the epithelial-mesenchymal transition (EMT) of respiratory tract and the intervention of saturated hydrogen on it.

Methods: 80 five-week-old C57BL6/J male mice were randomly divided into normal control group, H group, high-fat (HF) group and HF+H group, making 20 mice in each group. The weights of the mice were measured on weekly basis. Read More

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ZNRD1-AS1 knockdown alleviates malignant phenotype of retinoblastoma through miR-128-3p/BMI1 axis.

Am J Transl Res 2021 15;13(6):5866-5879. Epub 2021 Jun 15.

First Department of Oncology, Zhumadian Central Hospital Zhumadian, Henan, China.

Background: ZNRD1-AS1 plays an important role in liver cancer, endometrial cancer and other diseases. However, the relationship between ZNRD1-AS1 and retinoblastoma has not been studied in detail. This study aimed to determine the role of ZNRD1-AS1 in retinoblastoma. Read More

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Spironolactone Ameliorates Senescence and Calcification by Modulating Autophagy in Rat Tendon-Derived Stem Cells via the NF-B/MAPK Pathway.

Oxid Med Cell Longev 2021 30;2021:5519587. Epub 2021 Jun 30.

Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, Zhejiang Province, China.

Tendinopathy is a disabling musculoskeletal disease, the pathological process of which is tightly associated with inflammation. Spironolactone (SP) has been widely used as a diuretic in clinical practice. Recently, SP has shown anti-inflammatory features in several diseases. Read More

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Gonadotropin inhibitory hormone downregulates steroid hormone secretion and genes expressions in duck granulosa cells.

Anim Reprod 2021 19;18(2):e20210036. Epub 2021 Jul 19.

Zhongkai University of Agriculture and Engineering, Guangdong Guangzhou, China.

The mechanisms by which GnIH regulates the steroid synthesis pathway in duck granulosa cells remain poorly understood. In this study, we measured steroid hormone secretion by ELISA and reproduction-associated gene expression by quantitative real-time Polymerase Chain Reaction (qPCR) in duck granulosa cells treated with different concentrations of GnIH (0, 0.1, 1, 10, and 100 ng/mL) for 24 h. Read More

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Epitranscriptomic Analysis of m6A Methylome After Peripheral Nerve Injury.

Front Genet 2021 9;12:686000. Epub 2021 Jul 9.

Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China.

N6-methyladenosine (m6A) is one of the most plentiful internal RNA modifications, especially in eukaryotic messenger RNA (mRNA), which plays pivotal roles in the regulation of mRNA life cycle and nerve development. However, the mRNA m6A methylation pattern in peripheral nervous injury (PNI) has not been investigated. In this study, sciatic nerve samples were collected from 7 days after sciatic nerve injury (SNI) and control rats. Read More

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A Novel circRNA-miRNA-mRNA Hub Regulatory Network in Lung Adenocarcinoma.

Front Genet 2021 7;12:673501. Epub 2021 Jul 7.

Department of Radiation Oncology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

The growing evidence suggests that circular RNAs (circRNAs) have significant associations with tumor occurrence and progression, yet the regulatory mechanism of circRNAs in lung adenocarcinoma (LUAD) remains unclear. This study clarified the potentially regulatory network and functional mechanism of circRNAs in LUAD. The expression data of circRNAs, microRNAs (miRNAs), and messenger RNAs (mRNAs) were obtained from the Gene Expression Omnibus (GEO) database. Read More

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A Stable Cell Line Expressing Clustered AChR: A Novel Cell-Based Assay for Anti-AChR Antibody Detection in Myasthenia Gravis.

Front Immunol 2021 8;12:666046. Epub 2021 Jul 8.

Department of Neurology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Cell-based assays (CBAs) and radioimmunoprecipitation assay (RIPA) are the most sensitive methods for identifying anti-acetylcholine receptor (AChR) antibody in myasthenia gravis (MG). But CBAs are limited in clinical practice by transient transfection. We established a stable cell line (KL525) expressing clustered AChR by infecting HEK 293T cells with dual lentiviral vectors expressing the genes encoding the human AChR α1, β1, δ, ϵ and the clustering protein rapsyn. Read More

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Physiological, Genomic and Transcriptomic Analyses Reveal the Adaptation Mechanisms of to Extreme Acid Mine Drainage Environments.

Front Microbiol 2021 8;12:705839. Epub 2021 Jul 8.

Institute of Ecological Science, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China.

Fungi in acid mine drainage (AMD) environments are of great concern due to their potentials of decomposing organic carbon, absorbing heavy metals and reducing AMD acidity. Based on morphological analysis and ITS/18S high-throughput sequencing technology, previous studies have provided deep insights into the diversity and community composition of fungi in AMD environments. However, knowledge about physiology, metabolic potential and transcriptome profiles of fungi inhabiting AMD environments is still scarce. Read More

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A Four-MicroRNA Panel in Peripheral Blood Identified as an Early Biomarker to Diagnose Acute Myocardial Infarction.

Front Physiol 2021 7;12:669590. Epub 2021 Jul 7.

Department of Cardiology, The Second Affiliated Hospital of Nantong University, Nantong, China.

This study aimed to evaluate suitable circulating microRNAs (miRNAs) as diagnostic biomarkers of acute myocardial infarction (AMI). Patients with AMI were enrolled as study participants. All patients with AMI coming from the Second Affiliated Hospital of Nantong University between October 1, 2017 and May 31, 2019 were screened. Read More

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Age-Related Transcriptional Deregulation of Genes Coding Synaptic Proteins in Alzheimer's Disease Murine Model: Potential Neuroprotective Effect of Fingolimod.

Front Mol Neurosci 2021 9;14:660104. Epub 2021 Jul 9.

Laboratory of Preclinical Research and Environmental Agents, Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland.

Alzheimer's disease (AD) induces time-dependent changes in sphingolipid metabolism, which may affect transcription regulation and neuronal phenotype. We, therefore, analyzed the influence of age, amyloid β precursor protein (AβPP), and the clinically approved, bioavailable sphingosine-1-phosphate receptor modulator fingolimod (FTY720) on the expression of synaptic proteins. RNA was isolated, reverse-transcribed, and subjected to real-time PCR. Read More

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The Biological Function of TUSC7/miR-1224-3p Axis in Triple-Negative Breast Cancer.

Cancer Manag Res 2021 17;13:5763-5774. Epub 2021 Jul 17.

Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, People's Republic of China.

Background: Triple-negative breast cancers (TNBC), comprising about 20% of breast cancers, have a poor prognosis. Currently, there is no effective target therapy for TNBC. LncRNA TUSC7 has been identified as a tumor suppressor in osteosarcoma and colorectal cancer. Read More

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