141,564 results match your criteria genes encoding


Bioinformatics based discovery of new keratinases in protease family M36.

N Biotechnol 2022 Jan 12. Epub 2022 Jan 12.

Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, DTU Bioengineering, Building 221, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. Electronic address:

Keratinases are proteases that can catalyze the degradation of insoluble keratinous biomass. Keratinases in protease family M36 (MEROPS database) are endo-acting proteases. In total, 687 proteases are classified in family M36. Read More

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January 2022

Grouper USP12 exerts antiviral activity against nodavirus infection.

Fish Shellfish Immunol 2022 Jan 12. Epub 2022 Jan 12.

University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

The ubiquitin-specific proteases (USPs) have attracted particular attention due to their multiple functions in different biological processes. USP12, a member of the USP family, has been demonstrated to exert critical roles in diverse cellular processes, including cell death, cancer and antiviral immunity. Here, we cloned a USP12 homolog from orange spotted grouper (Epinephelus coioides, E. Read More

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January 2022

Identification of ribosomal protein L24 (RPL24) from the oriental river prawn, Macrobrachium nipponense, and its roles in ovarian development.

Comp Biochem Physiol A Mol Integr Physiol 2022 Jan 12:111154. Epub 2022 Jan 12.

Engineering Lab of Henan Province for Aquatic Animal Disease Control, Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.

Ribosomal proteins exhibit various extraribosomal functions in addition to their roles in protein synthesis. In this study, complementary DNA (cDNA) of ribosomal protein L24 in Macrobrachium nipponense (MnRPL24) was isolated, and its role in ovarian development was investigated using quantitative real-time PCR (qPCR), immunohistochemistry (IHC), RNA interference (RNAi) and histological observations. The complete cDNA of MnRPL24 is 564 base pairs (bps) and contains a 486 bp open reading frame (ORF) encoding 162 amino acids (aas). Read More

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January 2022

Association of ultra-rare coding variants with genetic generalized epilepsy: A case-control whole exome sequencing study.

Epilepsia 2022 Jan 15. Epub 2022 Jan 15.

Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Belvaux, Luxembourg.

Objective: We aimed to identify genes associated with genetic generalized epilepsy (GGE) by combining large cohorts enriched with individuals with a positive family history. Secondarily, we set out to compare the association of genes independently with familial and sporadic GGE.

Methods: We performed a case-control whole exome sequencing study in unrelated individuals of European descent diagnosed with GGE (previously recruited and sequenced through multiple international collaborations) and ancestry-matched controls. Read More

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January 2022

Characterization of the genome and serine protease of a novel Bacillus subtilis isolate.

Antonie Van Leeuwenhoek 2022 Jan 15. Epub 2022 Jan 15.

Department of Microbiology, Federal Urdu University of Arts, Science and Technology, Karachi, 75300, Pakistan.

Current study was undertaken to carry out the genome-wide analysis of a multipotent isolate from desert soil which was previously identified as Bacillus tequilensis based on 16S rDNA analysis. This study also aims to characterize the serine protease and its biocatalytic potentials implying a combination of empirical and in-silico approaches. Next generation sequencing and short read de novo assembly generated the 4,235,084 bp draft genome of Bacillus sp. Read More

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January 2022

The role of Even-skipped in Drosophila larval somatosensory circuit assembly.

eNeuro 2022 Jan 10. Epub 2022 Jan 10.

Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago IL, 60637

Proper somatosensory circuit assembly is critical for processing somatosensory stimuli and for responding accordingly. In comparison to other sensory circuits (e.g. Read More

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January 2022

In vitro and in silico analysis of Brilliant Black degradation by Actinobacteria and a Paraburkholderia sp.

Genomics 2022 Jan 11. Epub 2022 Jan 11.

Microbial Biotechnology, Ruhr-Universität Bochum, Universitätsstr. 150, 44780 Bochum, Germany. Electronic address:

The soil bacteria isolated in this study, including three strains of actinobacteria and one Paraburkholderia sp., showed decolorization activity of azo dyes in the resting cell assay and were shown to use methyl red as the sole carbon source to proliferate. Therefore, their ability to degrade, bioabsorb, or a combination of both was investigated using the substrate brilliant black. Read More

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January 2022

Native and foreign mitochondrial and nuclear encoded proteins conform the OXPHOS complexes of a holoparasitic plant.

Gene 2022 Jan 11:146176. Epub 2022 Jan 11.

IBAM, Universidad Nacional de Cuyo, CONICET, Facultad de Ciencias Agrarias, Almirante Brown 500, Chacras de Coria, M5528AHB, Mendoza, Argentina; Facultad de Ciencias Exactas y Naturales, Padre Jorge Contreras 1300, Universidad Nacional de Cuyo, M5502JMA, Mendoza, Argentina. Electronic address:

The intimate contact between the holoparasitic plant Lophophytum mirabile (Balanophoraceae) and its host plant (Fabaceae) facilitates the exchange of genetic information, increasing the frequency of horizontal gene transfer (HGT). Lophophytum stands out as it acquired a large number of mitochondrial genes (>20) from its legume host that replaced the majority of the native homologs. These foreign genes code for proteins that form multisubunit enzyme complexes, such as those in the oxidative phosphorylation system (OXPHOS) and cytochrome c maturation (ccm) system, together with dozens of nuclear-encoded subunits. Read More

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January 2022

Genetic analysis of pancreatic phospholipase A2 (PLA2G1B) in patients with chronic pancreatitis.

Pancreatology 2022 Jan 7. Epub 2022 Jan 7.

Pediatric Nutritional Medicine & Else Kröner-Fresenius-Centre for Nutritional Medicine (EKFZ), Technical University Munich (TUM), Freising, Germany. Electronic address:

Background: Genetic mutations in various pancreatic enzymes or their counteracting proteins have been linked to chronic pancreatitis. In particular, variants in the genes encoding pancreatic lipase (PNLIP) and carboxyl ester lipase (CEL) have been associated with pancreatitis. Therefore, we investigated pancreatic phospholipase A2 (PLA2G1B) as a promising candidate gene in patients with chronic pancreatitis. Read More

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January 2022

Molecular characterisation of carbapenem-resistant Klebsiella pneumoniae clinical isolates: preliminary experience from a tertiary care teaching hospital in the Himalayas.

Trans R Soc Trop Med Hyg 2022 Jan 13. Epub 2022 Jan 13.

Central Research Laboratory, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India 560070.

Background: There is a lack of whole-genome sequencing (WGS) data on multidrug-resistant (MDR) bacteria from the Uttarakhand region of India. The aim of this study was to generate WGS data of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates recovered from patients in Uttarakhand's tertiary care centre.

Methods: A cross-sectional study included 29 MDR K. Read More

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January 2022

Dual role of Ovol2 on the germ cell lineage segregation during gastrulation in mouse embryogenesis.

Development 2022 Jan 14. Epub 2022 Jan 14.

Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.

In mammals, primordial germ cells (PGCs), the origin of the germ line, are specified from the epiblast at the posterior region where gastrulation simultaneously occurs, yet the functional relationship between PGC specification and gastrulation remains unclear. Here, we show that Ovol2, a transcription factor conserved across the animal kingdom, balances these major developmental processes by repressing the epithelial-to-mesenchymal transition (EMT) driving gastrulation and the upregulation of genes associated with PGC specification. Ovol2a, a splice variant encoding a repressor domain, directly regulates EMT-related genes and consequently induces re-acquisition of potential pluripotency during PGC specification, whereas Ovol2b, another splice variant missing the repressor domain, directly upregulates genes associated with PGC specification. Read More

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January 2022

Transcription factor Mesenchyme Homeobox Protein 2 (MEOX2) modulates nociceptor function.

FEBS J 2022 Jan 13. Epub 2022 Jan 13.

Ludwig Boltzmann Institute for Rare and Undiagnosed Diseases, Vienna, Austria.

Mesenchyme homeobox protein 2 (MEOX2) is a transcription factor involved in mesoderm differentiation, including development of bones, muscles, vasculature and dermatomes. We have previously identified dysregulation of MEOX2 in fibroblasts from Congenital Insensitivity to Pain (CIP) patients, and confirmed that btn, the Drosophila homologue of MEOX2, plays a role in nocifensive responses to noxious heat stimuli. To determine the importance of MEOX2 in the mammalian peripheral nervous system, we used a Meox2 heterozygous (Meox2 ) mouse model to characterize its function in the sensory nervous system, and more specifically, in nociception. Read More

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January 2022

Metabolic adaptations in cancers expressing isocitrate dehydrogenase mutations.

Cell Rep Med 2021 Dec 21;2(12):100469. Epub 2021 Dec 21.

Chemistry Research Laboratory, 12 Mansfield Road, Department of Chemistry, University of Oxford, Oxford OX1 3TA, UK.

The most frequently mutated metabolic genes in human cancer are those encoding the enzymes isocitrate dehydrogenase 1 (IDH1) and IDH2; these mutations have so far been identified in more than 20 tumor types. Since mutations were first reported in glioma over a decade ago, extensive research has revealed their association with altered cellular processes. Mutations in lead to a change in enzyme function, enabling efficient conversion of 2-oxoglutarate to 2-hydroxyglutarate (2-HG). Read More

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December 2021

Crosstalk of DNA Methylation Triggered by Pathogen in Poplars With Different Resistances.

Front Microbiol 2021 28;12:750089. Epub 2021 Dec 28.

National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.

DNA methylation plays crucial roles in responses to environmental stimuli. Modification of DNA methylation during development and abiotic stress responses has been confirmed in increasing numbers of plants, mainly annual plants. However, the epigenetic regulation mechanism underlying the immune response to pathogens remains largely unknown in plants, especially trees. Read More

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December 2021

Peripheral NF-κB dysregulation in people with schizophrenia drives inflammation: putative anti-inflammatory functions of NF-κB kinases.

Transl Psychiatry 2022 Jan 13;12(1):21. Epub 2022 Jan 13.

Neuroscience Research Australia, Sydney, New South Wales, Australia.

Elevations in plasma levels of pro-inflammatory cytokines and C-reactive protein (CRP) in patient blood have been associated with impairments in cognitive abilities and more severe psychiatric symptoms in people with schizophrenia. The transcription factor nuclear factor kappa B (NF-κB) regulates the gene expression of pro-inflammatory factors whose protein products trigger CRP release. NF-κB activation pathway mRNAs are increased in the brain in schizophrenia and are strongly related to neuroinflammation. Read More

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January 2022

Genomic loci mispositioning in Tmem120a knockout mice yields latent lipodystrophy.

Nat Commun 2022 Jan 13;13(1):321. Epub 2022 Jan 13.

Institute of Cell Biology, University of Edinburgh, Edinburgh, EH9 3BF, UK.

Little is known about how the observed fat-specific pattern of 3D-spatial genome organisation is established. Here we report that adipocyte-specific knockout of the gene encoding nuclear envelope transmembrane protein Tmem120a disrupts fat genome organisation, thus causing a lipodystrophy syndrome. Tmem120a deficiency broadly suppresses lipid metabolism pathway gene expression and induces myogenic gene expression by repositioning genes, enhancers and miRNA-encoding loci between the nuclear periphery and interior. Read More

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January 2022

Computational and Experimental Characterization of rDNA and rRNA G-Quadruplexes.

J Phys Chem B 2022 Jan 13. Epub 2022 Jan 13.

Department of Physics, Wake Forest University, Winston-Salem, North Carolina 27109, United States.

DNA G-quadruplexes in human telomeres and gene promoters are being extensively studied for their role in controlling the growth of cancer cells. G-quadruplexes have been unambiguously shown to exist both and , including in the guanine (G)-rich DNA genes encoding pre-ribosomal RNA (pre-rRNA), which is transcribed in the cell's nucleolus. Recent studies strongly suggest that these DNA sequences ("rDNA"), and the transcribed rRNA, are a potential anticancer target through the inhibition of RNA polymerase I (Pol I) in ribosome biogenesis, but the structures of ribosomal G-quadruplexes at atomic resolution are unknown and very little biophysical characterization has been performed on them to date. Read More

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January 2022

Comparative analysis of ketone body metabolism in BALB/c mice infected with Trypanosoma evansi and Toxoplasma gondii.

Res Vet Sci 2021 Dec 20;143:134-141. Epub 2021 Dec 20.

Key Laboratory of Livestock Infectious Diseases, Ministry of Education, China; College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. Electronic address:

KBs (ketone bodies), i.e., acetoacetate, acetone, and (R)-3-Hydroxybutanoate, constitute the intermediate products of the incomplete oxidative degradation of fatty acids. Read More

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December 2021

Schistosoma mansoni α-N-acetylgalactosaminidase (SmNAGAL) regulates coordinated parasite movement and egg production.

PLoS Pathog 2022 Jan 13;18(1):e1009828. Epub 2022 Jan 13.

Institute of Biological, Environmental and Rural Sciences (IBERS), Edward Llwyd Building, Aberystwyth University, Aberystwyth, United Kingdom.

α-galactosidase (α-GAL) and α-N-acetylgalactosaminidase (α-NAGAL) are two glycosyl hydrolases responsible for maintaining cellular homeostasis by regulating glycan substrates on proteins and lipids. Mutations in the human genes encoding either enzyme lead to neurological and neuromuscular impairments seen in both Fabry- and Schindler/Kanzaki- diseases. Here, we investigate whether the parasitic blood fluke Schistosoma mansoni, responsible for the neglected tropical disease schistosomiasis, also contains functionally important α-GAL and α-NAGAL proteins. Read More

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January 2022

Evolutionary paths to macrolide resistance in a Neisseria commensal converge on ribosomal genes through short sequence duplications.

PLoS One 2022 13;17(1):e0262370. Epub 2022 Jan 13.

Rochester Institute of Technology, Thomas H. Gosnell School of Life Sciences, Rochester, NY, United States of America.

Neisseria commensals are an indisputable source of resistance for their pathogenic relatives. However, the evolutionary paths commensal species take to reduced susceptibility in this genus have been relatively underexplored. Here, we leverage in vitro selection as a powerful screen to identify the genetic adaptations that produce azithromycin resistance (≥ 2 μg/mL) in the Neisseria commensal, N. Read More

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January 2022

Chlorophyll and Pheophytin Dephytylating Enzymes Required for Efficient Repair of PSII in Synechococcus elongatus PCC 7942.

Plant Cell Physiol 2022 Jan 12. Epub 2022 Jan 12.

Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.

The CLD1 and PPH proteins of Arabidopsis thaliana are homologous proteins characterized respectively as a dephytylase for chlorophylls (Chls) and pheophytin a (Phein a) and a Phein a-specific dephytylase. Three genes encoding CLD1/PPH homologs (dphA1, dphA2, dphA3) were found in the genome of the cyanobacterium Synechococcus elongatus PCC 7942 and shown to be conserved in most cyanobacteria. His6-tagged DphA1, DphA2, and DphA3 proteins were expressed in E. Read More

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January 2022

Novel Compound Heterozygous Variants in the Gene in a Russian Patient with Meier-Gorlin Syndrome.

Appl Clin Genet 2022 6;15:1-10. Epub 2022 Jan 6.

Molecular Genetics Laboratory № 3 The Shared Resource Centre "Genome", Federal State Budgetary Scientific Institution Research Centre for Medical Genetics named after Academician N.P. Bochkov, Moscow, Russian Federation.

Background: Meier-Gorlin syndrome (MGS) is a rare genetic syndrome inherited in an autosomal dominant or autosomal recessive manner. The disorder is characterized by bilateral microtia, absence or hypoplasia of the patella, and an intrauterine growth retardation as well as a number of other characteristic features. The cause of the disease is mutations in genes encoding proteins involved in the regulation of the cell cycle (, and ). Read More

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January 2022

The ß-ketoadipate pathway of Acinetobacter baumannii is involved in complement resistance and affects resistance against aromatic antibiotics.

Environ Microbiol Rep 2022 Jan 12. Epub 2022 Jan 12.

Department of Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Goethe-University Frankfurt am Main, Frankfurt, Germany.

Acinetobacter baumannii can thrive on a broad range of substrates such as sugars, alcohols, lipids, amino acids and aromatic compounds. The latter three are abundant in the human host and are potential candidates as carbon sources for the metabolic adaptation of A. baumannii to the human host. Read More

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January 2022

Spatial and temporal dynamics and potential pathogenicity of fecal coliforms in coastal shallow groundwater wells.

Environ Monit Assess 2022 Jan 12;194(2):89. Epub 2022 Jan 12.

Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, 11600, Montevideo, Uruguay.

Access to water through shallow groundwater wells is a common practice in coastal settlements. This, coupled with a lack of planning for wastewater disposal promotes fecal contamination of groundwater and poses a threat to human health. Here, the spatial and temporal dynamics of groundwater fecal contamination was evaluated during summer and winter (2013 and 2014) in a coastal protected area having a high touristic relevance (Cabo Polonio, Uruguay). Read More

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January 2022

Modular evolution of secretion systems and virulence plasmids in a bacterial species complex.

BMC Biol 2022 Jan 13;20(1):16. Epub 2022 Jan 13.

Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.

Background: Many named species as defined in current bacterial taxonomy correspond to species complexes. Uncertainties regarding the organization of their genetic diversity challenge research efforts. We utilized the Agrobacterium tumefaciens species complex (a. Read More

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January 2022

Antibiotic resistance, virulence, and phylogenetic analysis of Escherichia coli strains isolated from free-living birds in human habitats.

PLoS One 2022 12;17(1):e0262236. Epub 2022 Jan 12.

Department of Tropical Medicine and Parasitology-National Centre for Tropical Medicine, Faculty of Health Science with Institute of Maritime and Tropical Medicine, Medical University of Gdańsk, Gdynia, Poland.

Wild birds can be colonized by bacteria, which are often resistant to antibiotics and have various virulence profiles. The aim of this study was to analyze antibiotic resistance mechanisms and virulence profiles in relation to the phylogenetic group of E. coli strains that were isolated from the GI tract of wildfowl. Read More

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January 2022

A novel phage indirectly regulates diatom growth by infecting diatom-associated biofilm-forming bacterium.

Appl Environ Microbiol 2022 Jan 12:AEM0213821. Epub 2022 Jan 12.

Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.

Algae and heterotrophic bacteria have close and intricate interactions, which are regulated by multiple factors in the natural environment. Phages are the major factor determining bacterial mortality. However, their impacts on the algae-associated bacteria and thus on the algae-bacteria interactions are poorly understood. Read More

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January 2022

Identification of key tumor stroma-associated transcriptional signatures correlated with survival prognosis and tumor progression in breast cancer.

Breast Cancer 2022 Jan 12. Epub 2022 Jan 12.

Biomedical Informatics Research Lab, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, 211198, China.

Background: The aberrant expression of stromal gene signatures in breast cancer has been widely studied. However, the association of stromal gene signatures with tumor immunity, progression, and clinical outcomes remains lacking.

Methods: Based on eight breast tumor stroma (BTS) transcriptomics datasets, we identified differentially expressed genes (DEGs) between BTS and normal breast stroma. Read More

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January 2022

A sorghum genome-wide association study (GWAS) identifies a WRKY transcription factor as a candidate gene underlying sugarcane aphid (Melanaphis sacchari) resistance.

Planta 2022 Jan 12;255(2):37. Epub 2022 Jan 12.

Section of Cell and Developmental Biology, University of California at San Diego, 9500 Gilman Dr., La Jolla, CA, 92093-0116, USA.

Main Conclusion: A WRKY transcription factor identified through forward genetics is associated with sorghum resistance to the sugarcane aphid and through heterologous expression reduces aphid populations in multiple plant species. Crop plant resistance to insect pests is based on genetically encoded traits which often display variability across diverse germplasm. In a comparatively recent event, a predominant sugarcane aphid (SCA: Melanaphis sacchari) biotype has become a significant agronomic pest of grain sorghum (Sorghum bicolor). Read More

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January 2022

The PqsE-RhlR Interaction Regulates RhlR DNA Binding to Control Virulence Factor Production in .

Microbiol Spectr 2022 Jan 12:e0210821. Epub 2022 Jan 12.

Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York, USA.

Pseudomonas aeruginosa is an opportunistic pathogen that causes disease in immunocompromised individuals and individuals with underlying pulmonary disorders. P. aeruginosa virulence is controlled by quorum sensing (QS), a bacterial cell-cell communication mechanism that underpins transitions between individual and group behaviors. Read More

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January 2022