161,191 results match your criteria genes detected

Degradation of p53 by HPV16-E6 variants isolated from cervical cancer specimens of Moroccan women.

Gene 2021 May 10:145709. Epub 2021 May 10.

Biology and Medical Research Unit, National Centre for Energy, Nuclear Sciences and Techniques (CNESTEN), Rabat, Morocco. Electronic address:

Cervical cancer is the second most diagnosed cancer in Moroccan women. The main etiological factor for developing cervical cancer is the persistent infection with HPV16. Genetic studies have reported the occurrence of amino acid variations within the E6 oncoprotein that promotes host cell transformation by targeting p53 for degradation. Read More

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Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.

PLoS One 2021 13;16(5):e0251305. Epub 2021 May 13.

Environmental Science, Policy and Management, University of California, Berkeley, California, United States of America.

Self-compatibility has become the primary objective of most prune (Prunus domestica) breeding programs in order to avoid the problems related to the gametophytic self-incompatibility (GSI) system present in this crop. GSI is typically under the control of a specific locus., known as the S-locus. Read More

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Trigger and suppression of antiviral defenses by grapevine Pinot gris virus (GPGV): novel insights into virus-host interaction.

Mol Plant Microbe Interact 2021 May 13. Epub 2021 May 13.

University of Udine, 9316, Department of Agriculture, Food, Environmental and Animal Sciences, Udine, Italy;

Grapevine Pinot gris virus (GPGV) is an emerging trichovirus that has been putatively associated with a novel grapevine disease known as grapevine leaf mottling and deformation (GLMD). Yet the role of GPGV in GLMD disease is poorly understood since it has been detected both in symptomatic and symptomless grapevines. We exploited a recently constructed GPGV infectious clone (pRI::GPGV-vir) to induce an antiviral response in Nicotiana benthamiana plants. Read More

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Gene-disruption of the entomopathogenic fungus Beauveria bassiana incubated with dsRNA.

J Basic Microbiol 2021 May 13. Epub 2021 May 13.

Department of Agricultural Biology, Jeonbuk National University, Jeonju, Korea.

The species of Beauveria bassiana is widely used for the management of agricultural insect pests. In this study, we integrated egfp-double-stranded RNA (dsRNA) to a previously generated egfp-expressing B. bassiana transformant (Bb-egfp#3) using a protoplast integration method. Read More

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Lupus susceptibility region containing CDKN1B rs34330 mechanistically influences expression and function of multiple target genes, also linked to proliferation and apoptosis.

Arthritis Rheumatol 2021 May 13. Epub 2021 May 13.

Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.

Objective: A recent genome-wide association study (GWAS) reported a significant genetic association between rs34330 of cyclin-dependent kinase inhibitor 1B (CDKN1B) and risk of systemic lupus erythematosus (SLE) in Han Chinese. This study aims to validate the reported association and elucidate the biochemical mechanisms underlying the variant's effect.

Methods: We performed allelic association with SLE followed by meta-analysis across 11 independent cohorts (n=28,872). Read More

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Overexpression of hsa_circ_0001445 reverses oxLDL‑induced inhibition of HUVEC proliferation via SRSF1.

Mol Med Rep 2021 Jul 13;24(1). Epub 2021 May 13.

Department of Cardiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.

Atherosclerosis is a primary cause of multiple types of cardiovascular disease, including myocardial infarction. In addition, injury of human umbilical vein endothelial cells (HUVECs) can lead to the development of atherosclerosis. Circular (circ)RNAs participate in atherosclerosis. Read More

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The role of connexin 37 polymorphism in spontaneous abortion.

Physiol Res 2021 May 12. Epub 2021 May 12.

Department of Obstetrics and Gynecology, Motol University Hospital, Prague, Czech Republic.

Among unique cardiovascular risk factors in women are complications during pregnancy, including miscarriage. Important risk factor is also genetic background. One of powerful candidate genes for cardiovascular disease of atherosclerotic origin (aCVD) is gene for connexin 37 (Cx37) with strong gene-environment interaction including smoking status, that is also strong risk factor for complications in pregnancy including spontaneous abortion (SA). Read More

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Optical genome mapping, a promising alternative to gold standard cytogenetic approaches in a series of acute lymphoblastic leukemias.

Genes Chromosomes Cancer 2021 May 13. Epub 2021 May 13.

CHU, Service d'Hématologie Biologique, Amiens, Picardie, France.

Acute lymphoblastic leukemias (ALL) are characterized by a large number of cytogenetic abnormalities of clinical interest that require the use of several complementary techniques. Optical genome mapping (OGM) is based on analysis of ultra-high molecular weight DNA molecules that provides a high-resolution genome-wide analysis highlighting copy number and structural anomalies, including balanced translocations. We compared OGM to standard techniques (karyotyping, fluorescent in situ hybridization, single nucleotide polymorphism-array and reverse transcription multiplex ligation-dependent probe amplification) in 10 selected B or T-ALL. Read More

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Mucin 16 Promotes Colorectal Cancer Development and Progression Through Activation of Janus Kinase 2.

Dig Dis Sci 2021 May 12. Epub 2021 May 12.

Department of General Surgery, Anhui Provincial Hospital, Cheeloo College of Medicine, Shandong University, No. 27, Shanda South Road, Jinan, 250012, Shandong, People's Republic of China.

Background: Mucin 16 (MUC16), a cell surface-associated mucin, has been implicated to be upregulated in a large repertoire of malignances. However, its function in the pathogenesis of colorectal cancer (CRC) is unknown.

Aims: Here, we explored the regulatory role of MUC16 in CRC. Read More

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The Prevalence of in Hard Ticks in Europe and Their Role in Q Fever Transmission Revisited-A Systematic Review.

Front Vet Sci 2021 26;8:655715. Epub 2021 Apr 26.

Institute of Bacterial Infections and Zoonoses (IBIZ), Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Jena, Germany.

The zoonosis Q fever is caused by the obligate intracellular bacterium . Besides the main transmission route inhalation of contaminated aerosols, ticks are discussed as vectors since the first isolation of the pathogen from a tick. The rare detection of in ticks and the difficult differentiation of from -like endosymbionts (CLEs) are questioning the relevance of ticks in the epidemiology of Q fever. Read More

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Integrated metabolome and transcriptome revealed the flavonoid biosynthetic pathway in developing leaves.

PeerJ 2021 26;9:e11239. Epub 2021 Apr 26.

Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, College of Forestry, Hainan University, Haikou, Hainan, China.

Background: as a tropical horticultural crop has been widely used for medicinal herb, feed, and vegetable. Recently, increasing studies revealed that this species possesses multiple pharmacological properties. Notably, leaves possess an abundance of flavonoids, but the specific profiles of flavonoids and the mechanisms of fl avonoid bi osynthesis in developing leaves are largely unknown. Read More

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Prevalence and Relatedness of -Mediated Colistin-Resistant Isolated From Livestock and Farmers in Japan.

Front Microbiol 2021 26;12:664931. Epub 2021 Apr 26.

Department of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan.

Colistin is used to treat infectious diseases in humans and livestock; it has also been used as a feed additive for livestock for approximately 50 years. Since the plasmid-mediated colistin resistance gene was discovered in China in 2015, it has been detected worldwide, mainly in livestock. In this study, we investigated the prevalence and characteristics of -mediated colistin-resistant in livestock and farmers in Japan. Read More

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A metallo-β-lactamase enzyme for internal detoxification of the antibiotic thienamycin.

Sci Rep 2021 May 12;11(1):10062. Epub 2021 May 12.

IRD, APHM, MEPHI, IHU-Méditerranée Infection, Aix Marseille Univ, 19-21 Boulevard Jean Moulin, 13005, Marseille, France.

Thienamycin, the first representative of carbapenem antibiotics was discovered in the mid-1970s from soil microorganism, Streptomyces cattleya, during the race to discover inhibitors of bacterial peptidoglycan synthesis. Chemically modified into imipenem (N-formimidoyl thienamycin), now one of the most clinically important antibiotics, thienamycin is encoded by a thienamycin gene cluster composed of 22 genes (thnA to thnV) from S. cattleya NRRL 8057 genome. Read More

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GWAS analysis reveals distinct pathogenicity profiles of Australian Parastagonospora nodorum isolates and identification of marker-trait-associations to septoria nodorum blotch.

Sci Rep 2021 May 12;11(1):10085. Epub 2021 May 12.

Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Perth, WA, Australia.

The fungus Parastagonospora nodorum is the causal agent of septoria nodorum leaf blotch (SNB) and glume blotch which are common in many wheat growing regions in the world. The disease is complex and could be explained by multiple interactions between necrotrophic effectors secreted by the pathogen and matching susceptibility genes in wheat. An Australian P. Read More

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Unraveling the role of microRNA/isomiR network in multiple primary melanoma pathogenesis.

Cell Death Dis 2021 May 12;12(5):473. Epub 2021 May 12.

Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Bologna, Italy.

Malignant cutaneous melanoma (CM) is a potentially lethal form of skin cancer whose worldwide incidence has been constantly increasing over the past decades. During their lifetime, about 8% of CM patients will develop multiple primary melanomas (MPMs), usually at a young age and within 3 years from the first tumor/diagnosis. With the aim of improving our knowledge on MPM biology and pathogenesis, we explored the miRNome of 24 single and multiple primary melanomas, including multiple tumors from the same patient, using a small RNA-sequencing approach. Read More

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Genome Investigation of Urinary Strains and Their Relationship to Isolates of the Vaginal Microbiota.

mSphere 2021 May 12;6(3). Epub 2021 May 12.

Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA.

is a frequent member of the urogenital microbiota. Given the association between and bacterial vaginosis (BV), significant efforts have been focused on characterizing this species in the vaginal microbiota. However, also is a frequent member of the urinary microbiota. Read More

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Development and validation of a core genome multilocus sequence typing scheme to facilitate molecular surveillance.

J Clin Microbiol 2021 May 12. Epub 2021 May 12.

Institute of Hygiene, Microbiology and Environmental Medicine, Medical University Graz, Graz, Austria

causes the severe disease melioidosis. Whole genome-sequencing (WGS) based typing methods currently offer the highest resolution for molecular investigations of this genetically diverse pathogen. Still, its routine application in diagnostic laboratories is limited by the need for high computing power, bioinformatic skills and variable bioinformatic approaches, the latter affecting the results. Read More

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Dynamics of microcystins and saxitoxin in the Indian River Lagoon, Florida.

Harmful Algae 2021 Mar 21;103:102012. Epub 2021 Mar 21.

Harbor Branch Oceanographic Institute, Florida Atlantic University, 5600 US 1 N, Fort Pierce, Florida 34946, USA.

Harmful algal blooms that can produce toxins are common in the Indian River Lagoon (IRL), which covers ~250 km of Florida's east coast. The current study assessed the dynamics of microcystins and saxitoxin in six segments of the IRL: Banana River Lagoon (BRL), Mosquito Lagoon (ML), Northern IRL (NIRL), Central IRL (CIRL), Southern IRL (SIRL), and the St. Lucie Estuary (SLE). Read More

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Clinical Contribution of Next-Generation Sequencing Multigene Panel Testing for BRCA Negative High-Risk Patients With Breast Cancer.

Clin Breast Cancer 2021 Apr 12. Epub 2021 Apr 12.

Department of Medical Genetic, Ege University Faculty of Medicine, Izmir, Turkey.

Background: Breast cancer is the most common malignancy in women and thought to be hereditary in 10% of patients. Recent next-generation sequencing studies have increased the detection of pathogenic or likely pathogenic (P/LP) variants in genes other than BRCA1/2 in patients with breast cancer. This study evaluated pathogenic variants, likely pathogenic variants, and variants of unknown significance in 18 hereditary cancer susceptibility genes in patients with BRCA1/2-negative breast cancer. Read More

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ATF5 and HIF1α cooperatively activate HIF1 signaling pathway in esophageal cancer.

Cell Commun Signal 2021 May 12;19(1):53. Epub 2021 May 12.

Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology, Wuhan, 430030, China.

Background: Esophageal cancer (ESCA) is one of the most common cancers worldwide and has a very poor prognosis. Hypoxia-inducible factor 1 (HIF1) signaling pathway plays a critical role in tumorigenesis and is therefore considered a potential therapeutic target in the treatment of many cancers. Activating transcription factor 5 (ATF5) facilitates the expression of various genes and has been extensively studied for its potential role in cancer treatment. Read More

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Low expression of CHRDL1 and SPARCL1 predicts poor prognosis of lung adenocarcinoma based on comprehensive analysis and immunohistochemical validation.

Cancer Cell Int 2021 May 12;21(1):259. Epub 2021 May 12.

College of Life Sciences, University of the Chinese Academy of Sciences, Beijing, 100049, China.

Purpose: Exploring the molecular mechanisms of lung adenocarcinoma (LUAD) is beneficial for developing new therapeutic strategies and predicting prognosis. This study was performed to select core genes related to LUAD and to analyze their prognostic value.

Methods: Microarray datasets from the GEO (GSE75037) and TCGA-LUAD datasets were analyzed to identify differentially coexpressed genes in LUAD using weighted gene coexpression network analysis (WGCNA) and differential gene expression analysis. Read More

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Genome-wide analysis of DNA methylation and risk of cardiovascular disease in a Chinese population.

BMC Cardiovasc Disord 2021 May 12;21(1):240. Epub 2021 May 12.

National Clinical Research Center for Cardiovascular Diseases, State Key Laboratory of Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Fuwai Hospital, National Center for Cardiovascular Diseases, 167 Beilishi Road, Beijing, 100037, People's Republic of China.

Background: Systemic studies of association of genome-wide DNA methylated sites with cardiovascular disease (CVD) in prospective cohorts are lacking. Our aim was to identify DNA methylation sites associated with the risk of CVD and further investigate their potential predictive value in CVD development for high-risk subjects.

Methods: We performed an epigenome-wide association study (EWAS) to identify CpGs related to CVD development in a Chinese population. Read More

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Differential expression of miRNAs in the presence of B chromosome in the cichlid fish Astatotilapia latifasciata.

BMC Genomics 2021 May 12;22(1):344. Epub 2021 May 12.

Department of Structural and Functional Biology, Institute of Bioscience at Botucatu, São Paulo State University (UNESP), Botucatu, SP, 18618-689, Brazil.

Background: B chromosomes (Bs) are extra elements observed in diverse eukaryotes, including animals, plants and fungi. Although Bs were first identified a century ago and have been studied in hundreds of species, their biology is still enigmatic. Recent advances in omics and big data technologies are revolutionizing the B biology field. Read More

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[Rare thalassemia caused by novel nucleotide variants in the globin gene: four case reports and literature review].

Zhonghua Xue Ye Xue Za Zhi 2021 Apr;42(4):313-317

Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China.

To analyze the DNA sequences and clinical phenotypes of four cases with rare thalassemia to improve its recognition and accurate diagnosis. The DNA sequence characteristics of four cases with rare thalassemia diagnosed from May 2014 to December 2019 were retrospectively analyzed, and related literature was reviewed. The results of the routine gene test for thalassemia indicated that the common three type of deletion and three point mutations in hemoglobin alpha 1/2 (HBA1/A2) , and 16 point mutations in hemoglobin beta (HBB) gene were unable to be detected in cases 1-3, and case 4 was--SEA. Read More

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High-Throughput Screening of an Octanoic Acid Producer Strain Library Enables Detection of New Targets for Increasing Titers in .

ACS Synth Biol 2021 May 12. Epub 2021 May 12.

Institute of Molecular Biosciences, Faculty of Biological Sciences, Goethe University Frankfurt, Max-von-Laue Straße 9, 60438 Frankfurt am Main, Germany.

Octanoic acid is an industrially relevant compound with applications in antimicrobials or as a precursor for biofuels. Microbial biosynthesis through yeast is a promising alternative to current unsustainable production methods. To increase octanoic acid titers in , we use a previously developed biosensor that is based on the octanoic acid responsive promotor coupled to GFP. Read More

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Characterization of intermediate-sized insertions using whole-genome sequencing data and analysis of their functional impact on gene expression.

Hum Genet 2021 May 12. Epub 2021 May 12.

Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Intermediate-sized insertions are one of the structural variants contributing to genome diversity. However, due to technical difficulties in identifying them, their importance in disease pathogenicity and gene expression regulation remains unclear. We used whole-genome sequencing data of 174 Japanese samples to characterize intermediate-sized insertions using a highly-accurate insertion calling method (IMSindel software and joint-call recovery) and obtained a catalogue of 4254 insertions. Read More

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Detection of Extended-Spectrum Beta-Lactamase-Producing and Carbapenem-Resistant Bacteria from Mink Feces and Feed in the United States.

Foodborne Pathog Dis 2021 May 11:1-10. Epub 2021 May 11.

Utah Water Research Laboratory, Department of Civil and Environmental Engineering, Utah State University, Logan, Utah, USA.

Antibiotic-resistant infections caused by extended-spectrum β-lactamases (ESBLs) and carbapenemases are increasing worldwide. Bacteria resistant to extended-spectrum cephalosporins and last resort carbapenems have been reported from food animals and their environments. Other concentrated nonfood-producing animals such as mink farming can be a reservoir of bacteria resistant to these critically important antibiotics. Read More

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Genomic Analysis of Antibiotic-Resistant and -Susceptible Isolated from Bovine Sources in Maputo, Mozambique.

Foodborne Pathog Dis 2021 May 11. Epub 2021 May 11.

Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, USA.

This study reports a genomic analysis of isolates recovered from 25 bovine fecal composite samples collected from four different production units in Maputo city and around Maputo Province, Mozambique. The genomes were analyzed to determine the presence of antibiotic resistance genes (ARGs), genetic relatedness, and virulence factors known to cause diseases in humans. Whole-genome sequencing was conducted on 28 isolates using an Illumina NextSeq 500 sequencing platform. Read More

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Implications of the germline variants of DNA damage response genes detected by cancer precision medicine for radiological risk communication and cancer therapy decisions.

J Radiat Res 2021 May;62(Supplement_1):i44-i52

Laboratory of Molecular Radiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Large-scale cancer-associated gene testing is now being rapidly incorporated into clinical settings, and is leading to incidental identification of the germline variants present in cancer patients. Because many cancer susceptibility genes are related to DNA damage response and repair, the variants may reflect not only the susceptibility to cancer but also the genetically defined radiation sensitivity of the patients and their relatives. When the presence of a certain germline variant increases the risk for developing radiation toxicity or radiation-induced secondary cancers, it will greatly influence the clinical decision-making. Read More

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Detection of coronavirus in vampire bats (Desmodus rotundus) in southern Brazil.

Transbound Emerg Dis 2021 May 12. Epub 2021 May 12.

Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.

The vampire bat (Desmodus rotundus) is a hematophagous animal that feeds exclusively on the blood of domestic mammals. Vampire bat feeding habits enable their contact with mammalian hosts and may enhance zoonotic spillover. Moreover, they may carry several pathogenic organisms, including coronaviruses (CoVs), for which they are important hosts. Read More

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