1,970 results match your criteria genes'

Genotyping of Virulence Genes and : Regional and National Study.

Int J Microbiol 2021 29;2021:5540560. Epub 2021 Jun 29.

Microbiology and Immunology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

() plays a crucial role in the pathogenesis of gastritis, peptic ulcer, and gastric cancer. The presence of pathogenicity islands (PAI) genes contributes to the pathogenesis of many gastrointestinal disorders. Cytotoxin-associated gene A () and vacuolating cytotoxin gene () are the most known virulence genes in . Read More

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Gene Loss, Pseudogenization in Plastomes of Genus (), and Putative Selection for Adaptation to Environmental Conditions.

Front Genet 2021 8;12:674783. Epub 2021 Jul 8.

Department of Higher Plants, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.

is a large family with more than 1,600 species, belonging to 75 genera. The largest genus--is vast, comprising about a thousand species. species (as well as other members of the ) are widespread and diversified, they are adapted to a wide range of habitats from shady forests to open habitats like meadows, steppes, and deserts. Read More

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The regulation of 1-methylcyclopropene treatment on the subfamily genes expression of ethylene response factors in peaches during storage.

Acta Sci Pol Technol Aliment 2021 Jul-Sep;20(3):313-323

College of Food Science and Technology, Nanjing Agricultural University, Jiangsu, PR China.

Background: Ethylene response factors (ERFs) perform diverse functions in fruit development, ripening and senescence. However, the effects of postharvest treatments on ERF genes have not been widely investigated due to the lack of peach ERF genomic information. The aim of this study was to investigate the ERF genes' expression of freshly harvested peach during storage after 1-methylcyclopropene (1-MCP) treatment. Read More

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A comprehensive investigation on liver regeneration: a meta-analysis and systems biology approach.

Clin Exp Hepatol 2021 Jun 30;7(2):183-190. Epub 2021 Jun 30.

Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Aim Of The Study: Liver regeneration is one of the essential fields of regenerative medicine as a branch of tissue engineering and molecular biology that draws global researchers' attention. This study aims to conduct a systematic review and meta-analysis on the high-throughput gene expression microarray dataset of liver regeneration on the NCBI-GEO database to identify the significant genes and signaling pathways and confirm the genes from literature studies on associated diseases.

Material And Methods: We thoroughly searched the NCBI-GEO database to retrieve and screen the GEO microarray datasets' contents. Read More

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Bacteria tolerant to colistin in coastal marine environment: Detection, microbiome diversity and antibiotic resistance genes' repertoire.

Chemosphere 2021 Oct 21;281:130945. Epub 2021 May 21.

Department of Biology, Faculty of Science, University of Split, Split, Croatia. Electronic address:

The global spread of mobilized colistin resistance (mcr) genes in clinical and natural environments dangerously diminishes the effectiveness of this last-resort antibiotic, becoming an urgent health threat. We used a multidisciplinary approach to detect mcr-1 gene and colistin (CL)-resistant bacteria in seawater from two Croatian public beaches. Illumina-based sequencing of metagenomic 16S rRNA was used to assess the taxonomic, functional, and antibiotic resistance genes (ARGs) profiling of the bacterial community tolerant to CL regarding different culture-based isolation methodologies. Read More

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October 2021

Viral Load Difference between Symptomatic and Asymptomatic COVID-19 Patients: Systematic Review and Meta-Analysis.

Infect Dis Rep 2021 Jul 16;13(3):645-653. Epub 2021 Jul 16.

Department of Translational Medicine, University of Ferrara, 44121 Ferrara, Italy.

We conducted a systematic review and meta-analysis to investigate the possible difference in the SARS-CoV-2 viral load between asymptomatic and symptomatic COVID-19 patients. Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were followed in abstracting data and assessing validity. We searched MEDLINE, Scopus, Web of Science and Google Scholar for all investigations in the English language, reporting data on the threshold cycle (C) from real-time RT-PCR assays for the RNA-dependent RNA polymerase (), envelope () and nucleocapsid () SARS-CoV-2 genes in asymptomatic and symptomatic COVID-19 patients. Read More

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Overexpression of the Long Noncoding RNA Inhibits Non-Small Cell Lung Cancer Cell Proliferation and Invasion Through HIF-2a Pathway.

Cancer Biother Radiopharm 2021 Jul 20. Epub 2021 Jul 20.

Department of Radiology, Tuberculosis Hospital of Shaanxi Province, The Fifth People's Hospital of Shaanxi Province, Xi'an, China.

The role and molecular mechanism of long-chain noncoding RNAs (lncRNAs) in lung cancer remain to be elucidated. The aim of this study was to investigate the association between a long coding RNA hypoxia-inducible factor-2α (HIF-2α) promoter upstream transcript () and clinical characteristics of non-small cell lung cancer (NSCLC) and its regulatory role in NSCLC. The correlation between expression and pathological features of NSCLC was analyzed in NSCLC patient samples. Read More

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NF-kB decoy oligodeoxynucleotide preserves disc height in a rabbit anular-puncture model and reduces pain induction in a rat xenograft-radiculopathy model.

Eur Cell Mater 2021 Jul 20;41:90-109. Epub 2021 Jul 20.

Department of Orthopedic Surgery, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093,

While it is known that the degenerated intervertebral disc (IVD) is one of the primary reasons for low-back pain and subsequent need for medical care, there are currently no established effective methods for direct treatment. Nuclear factor-κB (NF-κB) is a transcription factor that regulates various genes' expression, among which are inflammatory cytokines, in many tissues including the IVD. NF-κB decoy is an oligodeoxynucleotide containing the NF-κB binding site that entraps NF-κB subunits, resulting in suppression of NF-κB activity. Read More

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H3K27Ac modification and gene expression in psoriasis.

J Dermatol Sci 2021 Jul 6. Epub 2021 Jul 6.

Laboratory of Molecular Cell Biology, Center for Cancer Research and Department of Medicine C, Sheba Medical Center, Tel Hashomer, Israel; Faculty of Medicine, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address:

Background: Numerous alterations in gene expression have been described in psoriatic lesions compared to uninvolved or healthy skin. However, the mechanisms which induce this altered expression remain unclear. Epigenetic modifications play a key role in regulating genes' expression. Read More

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-regulatory switches establish scale colour identity and pattern diversity in .

Elife 2021 Jul 19;10. Epub 2021 Jul 19.

Department of Zoology, University of Cambridge, Downing St., Cambridge, United Kingdom.

In butterflies, wing colour pattern diversity and scale types are controlled by a few genes of large effect that regulate colour pattern switches between morphs and species across a large mimetic radiation. One of these genes, , has been repeatedly associated with colour pattern evolution in butterflies. Here we carried out CRISPR knockouts in multiple species and show that is a major determinant of scale cell identity. Read More

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Dynamics of extended-spectrum beta-lactamase-producing colonization in long-term carriers following travel abroad.

Microb Genom 2021 Jul;7(7)

Département Biologie Computationnelle, Institut Pasteur, USR 3756 CNRS, F-75015 Paris, France.

Travel to tropical regions is associated with high risk of acquiring extended-spectrum beta-lactamase-producing (ESBL-E) that are typically cleared in less than 3 months following return. The conditions leading to persistent carriage that exceeds 3 months in some travellers require investigation. Whole-genome sequencing (Illumina MiSeq) was performed on the 82 ESBL-E isolates detected upon return and 1, 2, 3, 6 and 12 months later from the stools of 11 long-term (>3 months) ESBL-E carriers following travel abroad. Read More

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Selective activation of cannabinoid receptor 2 regulates Treg/Th17 balance to ameliorate neutrophilic asthma in mice.

Ann Transl Med 2021 Jun;9(12):1015

Department of Pulmonary and Critical Care Medicine, Hainan General Hospital, Haikou, China.

Background: The cannabinoid receptor 2 (CNR2) plays a critical role in relieving asthma, with the mechanism still unclear. We aimed to investigate the mechanism of the CNR2 agonist (β-caryophyllene, β-Car) in regulating the balance of regulatory T cells (Treg) and T helper cell 17 (Th17) and thus its role in asthma.

Methods: The study group of 50 pathogen-free female BALB/c mice were randomly divided at 6-8 weeks old into five groups of Control, Asthma, Asthma + β-Car (10 mg/kg), Asthma + β-Car + SR144528 (specific CNR2 antagonist, 3 mg/kg), and Asthma + β-Car + CMD178 (inhibitor of Treg cell, 10 mg/kg). Read More

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Bioinformatics analysis of C3 and CXCR4 demonstrates their potential as prognostic biomarkers in clear cell renal cell carcinoma (ccRCC).

BMC Cancer 2021 Jul 15;21(1):814. Epub 2021 Jul 15.

Department of Urology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, 310014, China.

Background: The molecular prognostic biomarkers of clear cell renal cell carcinoma (ccRCC) are still unknown. We aimed at researching the candidate biomarkers and potential therapeutic targets of ccRCC.

Methods: Three ccRCC expression microarray datasets (include GSE14762, GSE66270 and GSE53757) were downloaded from the gene expression omnibus (GEO) database. Read More

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MRN complex and cancer risk: old bottles, new wine.

Clin Cancer Res 2021 Jul 14. Epub 2021 Jul 14.

Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, Hospitalet de Llobregat, Barcelona.

The MRN complex, composed of MRE11A, RAD50, and NBN, mediates vital molecular functions to maintain genomic stability and hence protect against related disorders. Germline mutations in the MRN genes predispose to three different syndromes: Ataxia-Telangiectasia-Like Disorder (MRE11A deficiency), Nijmegen Breakage Syndrome (NBS) (NBN deficiency) and NBS-Like Disorder (RAD50 deficiency). The potential cancer component of these syndromes in addition to the close physical and functional proximity of the MRN complex to BRCA1 have promoted the MRN genes as candidate risk genes for developing breast cancer. Read More

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A graph convolutional neural network for gene expression data analysis with multiple gene networks.

Stat Med 2021 Jul 14. Epub 2021 Jul 14.

Division of Biostatistics, University of Minnesota, Minneapolis, Minnesota, USA.

Spectral graph convolutional neural networks (GCN) are proposed to incorporate important information contained in graphs such as gene networks. In a standard spectral GCN, there is only one gene network to describe the relationships among genes. However, for genomic applications, due to condition- or tissue-specific gene function and regulation, multiple gene networks may be available; it is unclear how to apply GCNs to disease classification with multiple networks. Read More

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Bioinformatics Analysis of Candidate Genes and Pathways Related to Hepatocellular Carcinoma in China: A Study Based on Public Databases.

Pathol Oncol Res 2021 26;27:588532. Epub 2021 Mar 26.

Department of Hepatology, Tianjin Second People's Hospital, Tianjin, China.

Hepatocellular carcinoma (HCC) is a highly aggressive malignant tumor of the digestive system worldwide. Chronic hepatitis B virus (HBV) infection and aflatoxin exposure are predominant causes of HCC in China, whereas hepatitis C virus (HCV) infection and alcohol intake are likely the main risk factors in other countries. It is an unmet need to recognize the underlying molecular mechanisms of HCC in China. Read More

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InpherNet accelerates monogenic disease diagnosis using patients' candidate genes' neighbors.

Genet Med 2021 Jul 6. Epub 2021 Jul 6.

Department of Computer Science, Stanford School of Engineering, Stanford, CA, USA.

Purpose: Roughly 70% of suspected Mendelian disease patients remain undiagnosed after genome sequencing, partly because knowledge about pathogenic genes is incomplete and constantly growing. Generating a novel pathogenic gene hypothesis from patient data can be time-consuming especially where cohort-based analysis is not available.

Methods: Each patient genome contains dozens to hundreds of candidate variants. Read More

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Genomic evidence of adaptive evolution in the reptilian SOCS gene family.

PeerJ 2021 24;9:e11677. Epub 2021 Jun 24.

College of Life Science, Qufu Normal University, Qufu, Shandong, China.

The suppressor of the cytokine signaling (SOCS) family of proteins play an essential role in inhibiting cytokine receptor signaling by regulating immune signal pathways. Although SOCS gene functions have been examined extensively, no comprehensive study has been performed on this gene family's molecular evolution in reptiles. In this study, we identified eight canonical SOCS genes using recently-published reptilian genomes. Read More

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Dose-Dependent Effects of Cold Atmospheric Argon Plasma on the Mesenchymal Stem and Osteosarcoma Cells In Vitro.

Int J Mol Sci 2021 Jun 24;22(13). Epub 2021 Jun 24.

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia.

The antimicrobial, anti-inflammatory and tissue-stimulating effects of cold argon atmospheric plasma (CAAP) accelerate its use in various fields of medicine. Here, we investigated the effects of CAAP at different radiation doses on mesenchymal stem cells (MSCs) and human osteosarcoma (MNNG/HOS) cells. We observed an increase in the growth rate of MSCs at sufficiently low irradiation doses (10-15 min) of CAAP, while the growth of MNNG/HOS cells was slowed down to 41% at the same irradiation doses. Read More

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N6-Methylandenosine-Related lncRNAs in Tumor Microenvironment Are Potential Prognostic Biomarkers in Colon Cancer.

Front Oncol 2021 11;11:697949. Epub 2021 Jun 11.

Department of General Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, China.

Background: LncRNA dysregulation and the tumor microenvironment (TME) have been shown to play a vital role in the progression and prognosis of colon cancer (CC). We aim to reveal the potential molecular mechanism from the perspective of lncRNA in the TME and provide the candidate biomarkers for CC prognosis.

Methods: ESTIMATE analysis was used to divide the CC patients into high and low immune or stromal score groups. Read More

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p38β - MAPK11 and its role in female cancers.

J Ovarian Res 2021 Jun 26;14(1):84. Epub 2021 Jun 26.

Division of Thoracic Surgery, The Royal Brompton & Harefield NHS Foundation Trust, Harefield Hospital, London, UB9 6JH, UK.

Background: The p38MAPK family of Mitogen Activated Protein Kinases are a group of signalling molecules involved in cell growth, survival, proliferation and differentiation. The widely studied p38α isoform is ubiquitously expressed and is implicated in a number of cancer pathologies, as are p38γ and p38δ. However, the mechanistic role of the isoform, p38β, remains fairly elusive. Read More

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Reemergence of reticuloendotheliosis virus and Marek's disease virus co-infection in Yellow-Chickens in Southern China.

Poult Sci 2021 Aug 12;100(8):101099. Epub 2021 Mar 12.

Institute for Poultry Science and Health, Guangxi University, Nanning, Guangxi 530004, China. Electronic address:

The reticuloendotheliosis virus (REV) and the Marek's disease virus (MDV) cause reticuloendotheliosis (RE) and Marek's disease (MD) in poultry, respectively. According to epidemiological results obtained in our laboratory from 2010 to 2017, the positive rates of REV and MDV co-infection remained at low levels. In the present study, during the period of October 2018 to July 2020, 4 clinical cases with high morbidity (5%-20%) and mortality (2%-10%), caused by the co-infection of REV and vv+ MDV-like strains, were diagnosed and analyzed by histopathological observation, cell cultures and detection with ELISA and IFA, and the PCR and by sequencing of the isolates' genes. Read More

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FINER: enhancing the prediction of tissue-specific functions of isoforms by refining isoform interaction networks.

NAR Genom Bioinform 2021 Jun 22;3(2):lqab057. Epub 2021 Jun 22.

Department of Computer Science and Engineering, University of California, Riverside, CA 92521, USA.

Annotating the functions of gene products is a mainstay in biology. A variety of databases have been established to record functional knowledge at the gene level. However, functional annotations at the isoform resolution are in great demand in many biological applications. Read More

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Cyclophosphamide Exposure Causes Long-Term Detrimental Effect of Oocytes Developmental Competence Through Affecting the Epigenetic Modification and Maternal Factors' Transcription During Oocyte Growth.

Front Cell Dev Biol 2021 7;9:682060. Epub 2021 Jun 7.

Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Cyclophosphamide (CTX) is widely used in various cancer therapies and in immunosuppression, and patients can still have babies after CTX chemotherapy. CTX directly causes primordial follicle loss with overactivation and DNA damage-induced apoptosis. Previous studies have shown that maternal exposure to CTX before conception increases the incidence of birth abnormalities and alters the methylation of genes in the oocytes of offspring. Read More

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Interplay between SARS-CoV-2-derived miRNAs, immune system, vitamin D pathway and respiratory system.

J Cell Mol Med 2021 Jun 22. Epub 2021 Jun 22.

Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

The new coronavirus pandemic started in China in 2019. The intensity of the disease can range from mild to severe, leading to death in many cases. Despite extensive research in this area, the exact molecular nature of virus is not fully recognized; however, according to pieces of evidence, one of the mechanisms of virus pathogenesis is through the function of viral miRNAs. Read More

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Biogenesis of a Bacteriophage Long Non-Contractile Tail.

J Mol Biol 2021 Jun 18;433(18):167112. Epub 2021 Jun 18.

Unité de Virologie Moléculaire et Structurale, Centre de Recherche de Gif, CNRS UPR 3296 and IFR115, CNRS, Gif-sur-Yvette, France; Institute for Integrative Biology of the Cell, Université Paris-Saclay, CEA, CNRS, 91198 Gif-sur-Yvette, France. Electronic address:

Siphoviruses are main killers of bacteria. They use a long non-contractile tail to recognize the host cell and to deliver the genome from the viral capsid to the bacterial cytoplasm. Here, we define the molecular organization of the Bacillus subtilis bacteriophage SPP1 ~ 6. Read More

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Identifying HLA DRB1-DQB1 alleles associated with Chlamydia trachomatis infection and in silico prediction of potentially-related peptides.

Sci Rep 2021 Jun 18;11(1):12837. Epub 2021 Jun 18.

Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), 111321, Bogotá D.C., Colombia.

HLA class II (HLA-II) genes' polymorphism influences the immune response to Chlamydia trachomatis (Ct), it is considered a sexually transmitted infection. However, associations between HLA-II alleles and Ct-infection have been little explored in humans; this study was thus aimed at determining HLA-DRB1-DQB1 alleles/haplotypes' effect on Ct-infection outcome in a cohort of Colombian women. Cervical sample DNA was used as template for detecting Ct by PCR and typing HLA-DRB1-DQB1 alleles/haplotypes by Illumina MiSeq sequencing. Read More

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Cartilage tissue engineering by co-transplantation of chondrocyte extracellular vesicles and mesenchymal stem cells, entrapped in chitosan-hyaluronic acid hydrogel.

Biomed Mater 2021 Jul 13;16(5). Epub 2021 Jul 13.

Department of Cell Therapy and Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Mesenchymal stem cells (MSCs) on injectable hydrogels are mostly used to regenerate articular cartilage, which would have a variety of outcomes. Chondrocyte extracellular vesicles (EVs) have attracted many attentions for their chondrogenic differentiation capacity; however, the roles of EVs in both chondrogenic differentiation of MSCs and cartilage regeneration are poorly understood yet. In the current study, to investigate the differentiation effects of human articular chondrocyte EVs on adipose-derived MSCs, they were cultured in injectable chitosan-hyaluronic acid (CS-HA) hydrogel and then treated with chondrocyte EVs for 21 days. Read More

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Integrated analysis reveals the participation of IL4I1, ITGB7, and FUT7 in reshaping the TNBC immune microenvironment by targeting glycolysis.

Ann Med 2021 12;53(1):916-928

Key Laboratory of the Ministry of Education, Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The overall response rate of immunotherapy in triple-negative breast cancer (TNBC) remains unsatisfactory. Accumulating evidence indicated that glucose metabolic reprogramming could modulate immunotherapy efficacy. However, transcriptomic evidence remains insufficient. Read More

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December 2021

Identification of new semen trait-related candidate genes in Duroc boars through genome-wide association and weighted gene co-expression network analyses.

J Anim Sci 2021 Jul;99(7)

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China.

Semen traits are crucial in commercial pig production since semen from boars is widely used in artificial insemination for both purebred and crossbred pig production. Revealing the genetic architecture of semen traits potentially promotes the efficiencies of improving semen traits through artificial selection. This study is aimed to identify candidate genes related to the semen traits in Duroc boars. Read More

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