164 results match your criteria generation ids

Ictal wavefront propagation in slices and simulations with conductance-based refractory density model.

PLoS Comput Biol 2022 Jan 18;18(1):e1009782. Epub 2022 Jan 18.

Laboratory of Molecular Mechanisms of Neural Interactions, Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences, Saint Petersburg, Russia.

The mechanisms determining ictal discharge (ID) propagation are still not clear. In the present study, we aimed to examine these mechanisms in animal and mathematical models of epileptiform activity. Using double-patch and extracellular potassium ion concentration recordings in rat hippocampal-cortical slices, we observed that IDs moved at a speed of about 1 mm/s or less. Read More

View Article and Full-Text PDF
January 2022

Co-detection of the measles vaccine and wild-type virus by real-time PCR: public health laboratory protocol.

Access Microbiol 2021 5;3(11):000283. Epub 2021 Nov 5.

Centre for Infectious Disease Research, Diagnostics and laboratory Surveillance (IDS), National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.

In rare cases vaccination with the measles virus vaccine genotype A (MeVA) may cause a vaccine reaction with clinical signs similar to infection with wild-type measles virus (MeVwt). Rapid differentiation between MeVA and MeVwt infection is important for taking adequate public health measures. Recently, a few MeVA real-time reverse-transcription quantitative PCR methods (RT-qPCRs) were described that can distinguish between MeVA and MeVwt. Read More

View Article and Full-Text PDF
November 2021

Strippable Polymeric Nanocomposites Comprising "Green" Chelates, for the Removal of Heavy Metals and Radionuclides.

Polymers (Basel) 2021 Nov 30;13(23). Epub 2021 Nov 30.

National Institute of Materials Physics, 077125 Măgurele, Romania.

The issue of heavy metal and radionuclide contamination is still causing a great deal of concern worldwide for environmental protection and industrial sites remediation. Various techniques have been developed for surface decontamination aiming for high decontamination factors (DF) and minimal environmental impact, but strippable polymeric nanocomposite coatings are some of the best candidates in this area. In this study, novel strippable coatings for heavy metal and radionuclides decontamination were developed based on the film-forming ability of polyvinyl alcohol, with the remarkable metal retention capacity of bentonite nanoclay, together with the chelating ability of sodium alginate and with "new-generation" "green" complexing agents: iminodisuccinic acid (IDS) and 2-phosphonobutane-1,2,4-tricarboxylic acid (PBTC). Read More

View Article and Full-Text PDF
November 2021

"Men Are Scared That Others Will Know and Will Discriminate Against Them So They Would Rather Not Start Treatment." Perceptions of Heterosexual Men on HIV-Related Stigma in HIV Services in Blantyre, Malawi.

J Int Assoc Provid AIDS Care 2021 Jan-Dec;20:23259582211059921

Kamuzu University of Health Sciences, Blantyre, Malawi.

Background: Although the concept of treatment as prevention has generated optimism that an AIDS-free generation is within reach, the success of this approach centers upon early diagnosis and linkages to care for people living with HIV. Unfortunately, people continue to present for HIV care at late stages of disease and HIV-related stigma has been recognized as the major impediment to HIV prevention and treatment efforts. Given the relevance of addressing stigma to improve access and utilization of HIV services among men, this secondary analysis assessed perceptions of heterosexual men in HIV-related stigma on HIV testing and ART services in Blantyre District. Read More

View Article and Full-Text PDF
December 2021

Outcome without any adjuvant systemic treatment in stage I ER+/HER2- breast cancer patients included in the MINDACT trial.

Ann Oncol 2021 Dec 1. Epub 2021 Dec 1.

Department of Surgical Oncology, Netherlands Cancer Institute, Amsterdam, The Netherlands. Electronic address:

Background: Adjuvant systemic treatments (AST) reduce mortality, but have associated short- and long-term toxicities. Careful selection of patients likely to benefit from AST is needed. We evaluated outcome of low-risk breast cancer patients of the EORTC 10041/BIG 3-04 MINDACT trial who received no AST. Read More

View Article and Full-Text PDF
December 2021

Molecular analysis and novel variation identification of Chinese pedigrees with mucopolysaccharidosis using targeted next-generation sequencing.

Clin Chim Acta 2022 Jan 20;524:194-200. Epub 2021 Nov 20.

Obstetrics and Gynecology Department, Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, 450052 PR China. Electronic address:

Background: Mucopolysaccharidosis (MPS) refers to a group of lysosomal storage disorders for which seven types and 11 subtypes are currently recognized. Targeted next-generation sequencing (NGS) offers an important method of disease typing, diagnosis, prenatal diagnosis, and treatment.

Methods: Gene variations in 48 Chinese MPS patients were evaluated using NGS, and the pathogenicity of the DNA alterations was evaluated using PolyPhen2, SIFT, and Mutation Taster. Read More

View Article and Full-Text PDF
January 2022

Detection of Mosaic Variants in Mothers of MPS II Patients by Next Generation Sequencing.

Front Mol Biosci 2021 5;8:789350. Epub 2021 Nov 5.

Laboratory of Molecular Genetics, Medical Genetics Service, HCPA, Porto Alegre, Brazil.

Mucopolysaccharidosis type II is an X-linked lysosomal storage disorder caused by mutations in the gene that encodes the iduronate-2-sulfatase enzyme. The gene is located on the long arm of the X-chromosome, comprising 9 exons, spanning approximately 24 kb. The analysis of carriers, in addition to detecting mutations in patients, is essential for genetic counseling, since the risk of recurrence for male children is 50%. Read More

View Article and Full-Text PDF
November 2021

Validation, Implementation, and Clinical Utility of Whole Genome Sequence-Based Bacterial Identification in the Clinical Microbiology Laboratory.

J Mol Diagn 2021 11 9;23(11):1468-1477. Epub 2021 Aug 9.

Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, California. Electronic address:

The application of next-generation sequencing extends from microbial identification to epidemiologic insight and antimicrobial resistance prediction. Despite this potential, the roadblock for clinical laboratories lies in implementation and validation of such complex technology and data analysis. Herein, a validation study used whole-genome sequencing (WGS) for pan-bacterial identification (ID) in a clinical laboratory, and assessed its clinical relevance. Read More

View Article and Full-Text PDF
November 2021

Factors that influence enrollment in syringe services programs in rural areas: a qualitative study among program clients in Appalachian Kentucky.

Harm Reduct J 2021 06 30;18(1):68. Epub 2021 Jun 30.

Emory University Rollins School of Public Health, Atlanta, GA, USA.

Background: Enrolling sufficient number of people who inject drugs (PWID) into syringe services programs (SSP) is important to curtail outbreaks of drug-related harms. Still, little is known about barriers and facilitators to SSP enrollment in rural areas with no history of such programs. This study's purpose was to develop a grounded theory of the role of the risk environment and individual characteristics of PWID in shaping SSP enrollment in rural Kentucky. Read More

View Article and Full-Text PDF

Detailed pedigree analyses and prenatal diagnosis for a family with mucopolysaccharidosis type II.

BMC Med Genomics 2021 06 30;14(1):175. Epub 2021 Jun 30.

National Research Institute for Health and Family Planning, National Human Genetic Resources Center, Graduate School of Peking Union Medical College, Beijing, 100081, China.

Background: Mucopolysaccharidosis type II (MPS II) is an X-linked multisystem disorder caused by mutations in the gene encoding iduronate 2-sulfatase (IDS). The clinical manifestations of MPS II include skeletal deformities, airway obstruction, cardiomyopathy, and neurologic deterioration. MPS II has high genetic heterogeneity disorder, and ~ 658 variants of IDS have been reported. Read More

View Article and Full-Text PDF

Advancing clinical genomics and precision medicine with GVViZ: FAIR bioinformatics platform for variable gene-disease annotation, visualization, and expression analysis.

Hum Genomics 2021 06 26;15(1):37. Epub 2021 Jun 26.

Rutgers Institute for Health, Health Care Policy and Aging Research, Rutgers University, 112 Paterson Street, New Brunswick, NJ, USA.

Background: Genetic disposition is considered critical for identifying subjects at high risk for disease development. Investigating disease-causing and high and low expressed genes can support finding the root causes of uncertainties in patient care. However, independent and timely high-throughput next-generation sequencing data analysis is still a challenge for non-computational biologists and geneticists. Read More

View Article and Full-Text PDF

Impacts of climate change on yield of cereal crops in northern climatic region of Pakistan.

Environ Sci Pollut Res Int 2021 Nov 22;28(42):60235-60245. Epub 2021 Jun 22.

Department of Agricultural Economics, Sichuan Agriculture University, Chengdu, China.

This study investigates the impacts of climate change on yield of selected cereal crops (wheat and maize) in the northern climatic region of Khyber Pakhtunkhwa (KP) province of Pakistan for the period 1986-2015. The first-generation unit root tests such as the Levin, Lin, and Chu (LLC), augmented Dickey-Fuller (ADF)-Fisher, and the second-generation unit root tests such as cross-sectional augmented Im-Pesaran-Shin (CIPS) and cross-sectional ADF (CADF) are used to check stationarity of the series. The cointegration among the variables is discovered via Pedroni test and Westerlund method. Read More

View Article and Full-Text PDF
November 2021

Biases in study design, implementation, and data analysis that distort the appraisal of clinical benefit and ESMO-Magnitude of Clinical Benefit Scale (ESMO-MCBS) scoring.

ESMO Open 2021 06 20;6(3):100117. Epub 2021 Apr 20.

Cancer Pain and Palliative Medicine Service, Department of Medical Oncology, Shaare Zedek Medical Center, Jerusalem, Israel.

Background: The European Society for Medical Oncology-Magnitude of Clinical Benefit Scale (ESMO-MCBS) is a validated, widely used tool developed to score the clinical benefit from cancer medicines reported in clinical trials. ESMO-MCBS scores assume valid research methodologies and quality trial implementation. Studies incorporating flawed design, implementation, or data analysis may generate outcomes that exaggerate true benefit and are not generalisable. Read More

View Article and Full-Text PDF

Flame: an open source framework for model development, hosting, and usage in production environments.

J Cheminform 2021 Apr 19;13(1):31. Epub 2021 Apr 19.

Research Programme on Biomedical Informatics (GRIB), Department of Experimental and Health Sciences, Hospital del Mar Medical Research Institute (IMIM), Universitat Pompeu Fabra, Barcelona, Spain.

This article describes Flame, an open source software for building predictive models and supporting their use in production environments. Flame is a web application with a web-based graphic interface, which can be used as a desktop application or installed in a server receiving requests from multiple users. Models can be built starting from any collection of biologically annotated chemical structures since the software supports structural normalization, molecular descriptor calculation, and machine learning model generation using predefined workflows. Read More

View Article and Full-Text PDF

Internet of Things: Evolution, Concerns and Security Challenges.

Sensors (Basel) 2021 Mar 5;21(5). Epub 2021 Mar 5.

Department of Information Management, Oriental Institute of Technology, New Taipei City 22064, Taiwan.

The escalated growth of the Internet of Things (IoT) has started to reform and reshape our lives. The deployment of a large number of objects adhered to the internet has unlocked the vision of the smart world around us, thereby paving a road towards automation and humongous data generation and collection. This automation and continuous explosion of personal and professional information to the digital world provides a potent ground to the adversaries to perform numerous cyber-attacks, thus making security in IoT a sizeable concern. Read More

View Article and Full-Text PDF

Preimplantation genetic testing for a chr14q32 microdeletion in a family with Kagami-Ogata syndrome and Temple syndrome.

J Med Genet 2021 Feb 12. Epub 2021 Feb 12.

Cancer Epigenetics and Biology Program, Bellvitge Institute for Biomedical Research, Barcelona, Spain

Introduction: Kagami-Ogata syndrome (KOS14) and Temple syndrome (TS14) are two disorders associated with reciprocal alterations within the chr14q32 imprinted domain. Here, we present a work-up strategy for preimplantation genetic testing (PGT) to avoid the transmission of a causative micro-deletion.

Methods: We analysed DNA from the KOS14 index case and parents using methylation-sensitive ligation-mediated probe amplification and methylation pyrosequencing. Read More

View Article and Full-Text PDF
February 2021

GECO: gene expression clustering optimization app for non-linear data visualization of patterns.

BMC Bioinformatics 2021 Jan 25;22(1):29. Epub 2021 Jan 25.

Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, CA, 92697, USA.

Background: Due to continued advances in sequencing technology, the limitation in understanding biological systems through an "-omics" lens is no longer the generation of data, but the ability to analyze it. Importantly, much of this rich -omics data is publicly available waiting to be further investigated. Although many code-based pipelines exist, there is a lack of user-friendly and accessible applications that enable rapid analysis or visualization of data. Read More

View Article and Full-Text PDF
January 2021

Transcriptome characterization and generation of marker resource for Himalayan vulnerable species, Ulmus wallichiana.

Mol Biol Rep 2021 Jan 13;48(1):721-729. Epub 2021 Jan 13.

Molecular Genetics Laboratory, Department of Botany, Central University of Punjab, VPO Ghudda, Distt, Bathinda, 151401, India.

Ulmus wallichiana is a traditional medicinal plant listed as a vulnerable in the IUCN red list data. Genomic and transcriptomic resources for this species are lacking, hindering its genetic exploration. Further, no polymorphic marker resource is available for this species, thus limiting the elucidation of its underlying genetic diversity, which is a pre-requisite for its conservation. Read More

View Article and Full-Text PDF
January 2021

Rapid identification of anti-idiotypic mAbs with high affinity and diverse epitopes by rabbit single B-cell sorting-culture and cloning technology.

PLoS One 2020 21;15(12):e0244158. Epub 2020 Dec 21.

Department of Antibody Engineering, Genentech Inc., South San Francisco, CA, United States of America.

The proactive generation of anti-idiotypic antibodies (anti-IDs) against therapeutic antibodies with desirable properties is an important step in pre-clinical and clinical assay development supporting their bioanalytical programs. Here, we describe a robust platform to generate anti-IDs using rabbit single B cell sorting-culture and cloning technology by immunizing rabbits with therapeutic drug Fab fragment and sorting complementarity determining regions (CDRs) specific B cells using designed framework control as a negative gate to exclude non-CDRs-specific B cells. The supernatants of cultured B cells were subsequently screened for binding to drug-molecule by enzyme-linked immunosorbent assay and the positive hits of B cell lysates were selected for cloning of their immunoglobulin G (IgG) variable regions. Read More

View Article and Full-Text PDF

Association between polymorphism in gene related to the dopamine circuit and motivations for drinking in patients with alcohol use disorder.

Psychiatry Res 2021 01 7;295:113563. Epub 2020 Nov 7.

Department of Psychobiology, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu, 862 - 1o. andar, Zip code 04023-062, São Paulo, SP, Brazil.

The development of alcohol use disorder (AUD) is influenced by genetic, psychological, and social factors. However, the identification of the load of each of these factors and the association between them is still debatable. This study aimed to explore the load of the association between AUD and polymorphisms in genes of the dopaminergic system, as well as with drinking triggers. Read More

View Article and Full-Text PDF
January 2021

Why behaviours do not change: structural constraints that influence household decisions to control pig diseases in Myanmar.

Prev Vet Med 2020 Oct 8;183:105138. Epub 2020 Sep 8.

Myanmar Oxford University Research Clinical Unit, Kokkine swimming pool lane, Yangon, Myanmar. Electronic address:

This article problematises the ways in which behavioural change by poor individuals dominates the global discussions on means to tackle issues caused by systemic problems. We do so by focusing on the case of animal diseases, a symptom of many systematic failures. While livestock farming has long been praised as a mitigating factor against poverty, livestock diseases threaten the lives and livelihoods of poor and marginalised people who depend on animals. Read More

View Article and Full-Text PDF
October 2020

High-Throughput Liquid Chromatography-Tandem Mass Spectrometry Quantification of Glycosaminoglycans as Biomarkers of Mucopolysaccharidosis II.

Int J Mol Sci 2020 Jul 30;21(15). Epub 2020 Jul 30.

Denali Therapeutics, Inc., 161 Oyster Point Blvd, South San Francisco, CA 94080, USA.

We recently developed a blood-brain barrier (BBB)-penetrating enzyme transport vehicle (ETV) fused to the lysosomal enzyme iduronate 2-sulfatase (ETV:IDS) and demonstrated its ability to reduce glycosaminoglycan (GAG) accumulation in the brains of a mouse model of mucopolysaccharidosis (MPS) II. To accurately quantify GAGs, we developed a plate-based high-throughput enzymatic digestion assay coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to simultaneously measure heparan sulfate and dermatan sulfate derived disaccharides in tissue, cerebrospinal fluid (CSF) and individual cell populations isolated from mouse brain. The method offers ultra-high sensitivity enabling quantitation of specific GAG species in as low as 100,000 isolated neurons and a low volume of CSF. Read More

View Article and Full-Text PDF

Simulation of Human Small Intestinal Digestion of Starch Using an In Vitro System Based on a Dialysis Membrane Process.

Foods 2020 Jul 10;9(7). Epub 2020 Jul 10.

Department of Chemistry, Universidad Tecnológica Metropolitana, Las Palmeras 3360, Ñuñoa, Santiago 7800003, Chile.

This work deepens our understanding of starch digestion and the consequent absorption of hydrolytic products generated in the human small intestine. Gelatinized starch dispersions were digested with α-amylase in an in vitro intestinal digestion system (-IDS) based on a dialysis membrane process. This study innovates with respect to the existing literature, because it considers the impact of simultaneous digestion and absorption processes occurring during the intestinal digestion of starchy foods and adopts phenomenological models that deal in a more realistic manner with the behavior found in the small intestine. Read More

View Article and Full-Text PDF

Psychosocial and cultural needs of children with intellectual disability and their families among the Syrian refugee population in Turkey.

J Intellect Disabil Res 2020 08 6;64(8):644-656. Epub 2020 Jul 6.

Department of Mental Health, Ministry of Health, Ankara, Turkey.

Background: Turkey is the country hosting the largest number of refugees from Syria, with currently 3 571 175 million persons. The general health needs of the refugees are being addressed; however, people with intellectual disabilities (IDs), particularly children, are relatively missed. The aim of this study was to identify medical, psychological and social needs of children with ID and their families, among the Syrian refugee population in Turkey, and to define psychosocial and cultural needs for planning of future services. Read More

View Article and Full-Text PDF

[Identification of a novel splicing variant of IDS gene in a pedigree affected with type II glycosaminoglycan product storage disease].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Jul;37(7):713-716

Center for Reproductive Medicine, Shandong University; National Research Center for Assisted Reproductive Technology and Reproductive Genetics; Key Laboratory for Reproductive Endocrinology of Ministry of Education, China; Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, Shandong 250001, China.

Objective: To analyze variant of IDS gene in a pedigree affected with mucopolysaccharidosis type II (MPS II).

Methods: The proband was subjected to next generation sequencing and Sanger sequencing to identify potential variants. Suspected variant was analyzed by its co-segregation with the disease in the pedigree. Read More

View Article and Full-Text PDF

TopoTag: A Robust and Scalable Topological Fiducial Marker System.

IEEE Trans Vis Comput Graph 2021 09 29;27(9):3769-3780. Epub 2021 Jul 29.

Fiducial markers have been playing an important role in augmented reality (AR), robot navigation, and general applications where the relative pose between a camera and an object is required. Here we introduce TopoTag, a robust and scalable topological fiducial marker system, which supports reliable and accurate pose estimation from a single image. TopoTag uses topological and geometrical information in marker detection to achieve higher robustness. Read More

View Article and Full-Text PDF
September 2021

Identifying the Prescribing Physician in US Healthcare Claims Data.

Med Care 2020 06;58(6):e39-e44

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC.

Background: Identifying the prescribing physician may be essential in studies that use health care claims databases but often there is no single consistent physician identifier (ID) available that spans an entire database and no standard methods have emerged to address this issue.

Methods: Unique Drug Enforcement Administration (DEA) numbers (N=36,721) were identified from the pharmacy claims of a cohort of postmenopausal (55+) initiators of osteoporosis medications (2008-2011) in the United Healthcare database. The proportion of times a Provider ID appeared at least once on the outpatient medical service claims (OMSCs) in the 14 days before a new prescription (Rx) out of the total number of new Rxs for each DEA was calculated and the Provider ID with the highest proportion was considered the most likely match to the DEA. Read More

View Article and Full-Text PDF

Utilization of Technology to Improve Efficiency in Investigational Drug Management Processes.

J Pharm Technol 2020 Apr 22;36(2):84-90. Epub 2020 Jan 22.

University of Kansas Medical Center, Kansas City, KS, USA.

Background: An investigational pharmacy is responsible for all tasks related to receiving, storing, and dispensing of any investigational drugs. Traditional methods of inventory and protocol tracking on paper binders are very tedious and could be error-prone. To evaluate the utilization of the IDS to efficiently manage the inventory within an investigational Pharmacy. Read More

View Article and Full-Text PDF

Factors associated with infertility distress of infertile women: a cross-sectional study.

J Psychosom Obstet Gynaecol 2020 12 6;41(4):275-281. Epub 2020 Jan 6.

Midwifery, Süleymaniye Obstetrics and Gynecology Training and Research Hospital, Istanbul, Turkey.

Background: Women undergoing infertility treatment may suffer infertility distress.

Objective: The aim of this study was to determine factors associated with infertility distress in women with infertility problems.

Materials And Methods: Women ( = 368) who received infertility treatment and agreed to participate in the study constituted the sample of this cross-sectional descriptive study. Read More

View Article and Full-Text PDF
December 2020

Functional electrical stimulation of the facial muscles to improve symptoms in individuals with major depressive disorder: pilot feasibility study.

Biomed Eng Online 2019 Nov 14;18(1):109. Epub 2019 Nov 14.

Rehabilitation Engineering Laboratory, Lyndhurst Centre, KITE, Toronto Rehabilitation Institute-University Health Network, Toronto, ON, Canada.

Background: Currently, the mainstay of treatment in patients diagnosed with major depressive disorder (MDD) requiring medical attention is second generation anti-depressants. However, about 40% of patients treated with second-generation anti-depressants do not respond to initial treatment and approximately 70% do not achieve remission during the first-step treatment. There are a few non-pharmacological options available, but none have shown consistently positive results. Read More

View Article and Full-Text PDF
November 2019