302,996 results match your criteria gene transfer

RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy.

Cell Stem Cell 2021 Sep 14. Epub 2021 Sep 14.

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA; Center for Pharmacogenomics, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA. Electronic address:

Doxorubicin is an anthracycline chemotherapy agent effective in treating a wide range of malignancies, but its use is limited by dose-dependent cardiotoxicity. A recent genome-wide association study identified a SNP (rs2229774) in retinoic acid receptor-γ (RARG) as statistically associated with increased risk of anthracycline-induced cardiotoxicity. Here, we show that human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from patients with rs2229774 and who suffered doxorubicin-induced cardiotoxicity (DIC) are more sensitive to doxorubicin. Read More

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September 2021

Global Proximity Interactome of the Human Macroautophagy Pathway.

Autophagy 2021 Sep 15:1-13. Epub 2021 Sep 15.

Cell Biology Program, Hospital for Sick Children, Toronto, Ontario, Canada.

Abbreviations: BioID: proximity-dependent biotin identification; GO: gene ontology; OSBPL: oxysterol binding protein like; VAPA: VAMP associated protein A; VAPB: VAMP associated protein B and C. Read More

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September 2021

Gene expression profiles in the brain of phenylketonuria mouse model reversed by the low phenylalanine diet therapy.

Metab Brain Dis 2021 Sep 15. Epub 2021 Sep 15.

Department of Pediatric Endocrinology and Genetic Metabolism, Shanghai Institute for Pediatric Research, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Kongjiang Road 1665#, Shanghai, 200092, China.

To gain insight into the potential protective mechanisms of low phenylalanine diet (LPD) in phenylketonuria (PKU), gene expression profiles were studied in the cerebral cortex and hippocampus of a PKU mouse model (BTBR-Pah). PKU mice were fed with low Phe diet (LPD-PKU group) and normal diet (PKU group). Wild-type mice were treated with normal diet (WT group) as control. Read More

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September 2021

Hashimoto's thyroiditis and coexisting disorders in correlation with HLA status-an overview.

Wien Med Wochenschr 2021 Sep 15. Epub 2021 Sep 15.

Teaching Unit, Medizinische Universität Wien/Medical University Vienna, Vienna, Austria.

Hashimoto's thyroiditis (HT), also known as chronic lymphocytic thyroiditis, is a frequent disorder of the thyroid gland caused by autoimmune-trigged lymphocytic infiltration and destruction of the thyroid gland. With the progressive destruction of the organ, the thyroid gland shrinks in size, thus commonly leading to hypothyroidism. Therapy of HT is mainly focused on managing the thyroid dysfunction by oral substitution of L‑thyroxine. Read More

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September 2021

Identification of Species by Combining Molecular and Morphological Data Using Convolutional Neural Networks.

Syst Biol 2021 Sep 15. Epub 2021 Sep 15.

College of Life Sciences, Capital Normal University, Beijing 100048, People's Republic of China.

Integrative taxonomy is central to modern taxonomy and systematic biology, including behaviour, niche preference, distribution, morphological analysis and DNA barcoding. However, decades of use demonstrate that these methods can face challenges when used in isolation, for instance, potential misidentifications due to phenotypic plasticity for morphological methods, and incorrect identifications because of introgression, incomplete lineage sorting and horizontal gene transfer for DNA barcoding. Although researchers have advocated the use of integrative taxonomy, few detailed algorithms have been proposed. Read More

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September 2021

Genetic and epigenetic mechanisms involved in bariatric surgery.

Ann Ital Chir 2021 ;92:397-405

Morbid obesity (BMI>40 kg/m2) is a challenging health condition with an increasing incidence in the last decades. Conventional therapy which consists in diet and lifestyle interventions, along with pharmaceutical therapy, has a limited effect on morbidly obese patients. In this context, bariatric surgery is the most effective approach, leading to significant weight loss, along with other beneficial effects like type 2 diabetes resolution or improvement of cardiovascular status. Read More

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January 2021

A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis.

Microbiol Spectr 2021 Sep 15:e0075421. Epub 2021 Sep 15.

Department of Microbiology, Cornell Universitygrid.5386.8, Ithaca, New York, USA.

The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction. Conventional CRISPR-Cas9 editing strategies require design and molecular cloning of an appropriate guide RNA (gRNA) to target genome cleavage and a repair template for introduction of the desired site-specific genome modification. Here, we present a streamlined method that leverages the existing collection of nearly 4,000 Bacillus subtilis strains (the BKE collection) with individual genes replaced by an integrated erythromycin () resistance cassette. Read More

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September 2021

Genetic Determinants of Intrinsic Antibiotic Tolerance in Mycobacterium avium.

Microbiol Spectr 2021 Sep 15:e0024621. Epub 2021 Sep 15.

Center for Systems Approaches to Infectious Diseases (C-SAID), Johns Hopkins University School of Medicinegrid.471401.7, Baltimore, Maryland, USA.

The Mycobacterium avium complex (MAC) is one of the most prevalent causes of nontuberculous mycobacteria pulmonary infection in the United States, and yet it remains understudied. Current MAC treatment requires more than a year of intermittent to daily combination antibiotic therapy, depending on disease severity. In order to shorten and simplify curative regimens, it is important to identify the innate bacterial factors contributing to reduced antibiotic susceptibility, namely, antibiotic tolerance genes. Read More

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September 2021

[CRISPR gene therapy enters the clinic: the future starts now].

Ned Tijdschr Geneeskd 2021 Aug 30;165. Epub 2021 Aug 30.

Amsterdam UMC, locatie VUmc, afd. Humane Genetica, Amsterdam.

In 2020, the Nobel Prize in Chemistry was awarded to American molecular biologist Jennifer Doudna and her French colleague Emmanuelle Charpentier for their fundamental research on CRISPR, an ingenious bacterial immune system. Studies into the working mechanism of CRISPR led to many Eureka moments. Through smart biotechnological engineering, CRISPR became suitable for applications in 'DNA surgery': the targeted editing of the genetic code. Read More

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Independent of direct interspecies electron transfer: Magnetite-mediated sulphur cycle for anaerobic degradation of benzoate under low-concentration sulphate conditions.

J Hazard Mater 2021 Sep 3;423(Pt A):127051. Epub 2021 Sep 3.

Key Laboratory of Industrial Ecology and Environmental Engineering (Dalian University of Technology), Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.

The aim of this study was to investigate the primary mechanism of magnetite promoting anaerobic degradation of aromatic compounds under the low-concentration sulphate conditions. Under influent conditions of benzoate at 50 mM-chemical oxygen demand (COD) and sulphate at 15 mM, magnetite promoted benzoate degradation (77.1% vs 56. Read More

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September 2021

Reviewing pharmacogenetics to advance precision medicine for opioids.

Biomed Pharmacother 2021 Sep 12;142:112060. Epub 2021 Sep 12.

Department of Pharmacology and Toxicology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada; Department of Psychiatry, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada; Institute of Medical Sciences, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada; Centre for Addiction and Mental Health, Toronto, ON, Canada. Electronic address:

Background: Adequate opioid prescribing is critical for therapeutic success of pain management. Despite the widespread use of opioids, optimized opioid therapy remains unresolved with risk of accidental lethal overdosing. With the emergence of accumulating evidence linking genetic variation to opioid response, pharmacogenetic based treatment recommendations have been proposed. Read More

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September 2021

Targeted nanostructured lipid carrier containing galangin as a promising adjuvant for improving cytotoxic effects of chemotherapeutic agents.

Naunyn Schmiedebergs Arch Pharmacol 2021 Sep 15. Epub 2021 Sep 15.

Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Resistance to chemotherapeutic drugs is the main limitation of cancer therapy. The combination use of chemotherapeutic agents and galangin (a naturally active flavonoid) amplifies the effectiveness of cancer treatment. This study aimed to prepare arginyl-glycyl-aspartic acid (RGD) containing nanostructured lipid carrier (NLC-RGD) to improve the bioavailability of galangin and explore its ability in improving the cytotoxic effects of doxorubicin (DOX). Read More

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September 2021

Isolation of Bovine leukemia virus from cows with persistent lymphocytosis in Iraq.

Vet Anim Sci 2021 Dec 30;14:100201. Epub 2021 Aug 30.

Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriyah University, Baghdad, Iraq.

This is the first study to report on the isolation of bovine leukemia virus (BLV) from peripheral blood mononuclear cells of two cross bred cows in Iraq. The cattle were seropositive by ELISA when selected while being surveyed for the detection of BLV. Among six cows, two were cases of persistent lymphocytosis (PL). Read More

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December 2021

A review of the endocrine resistance in hormone-positive breast cancer.

Tsai-Ju Chien

Am J Cancer Res 2021 15;11(8):3813-3831. Epub 2021 Aug 15.

Division of Hemato-Oncology, Department of Internal Medicine, Branch of Zhong-Zhou, Taipei City Hospital Taipei, Taiwan.

Hormone-positive breast cancer (BC) is a unique heterogeneous disease with a favorable prognosis compared to other types of breast cancer. As tumor biology influences the prognosis and clinical treatment, a deep understanding of how the molecular mechanisms regulate hormone sensitivity or resistance is critical in improving the efficacy and overcoming the endocrine resistance. This article comprehensively reviews the endocrine resistance in hormone-positive BC from a molecular and genetic perspective, encompassing the updated treatment and developing direction. Read More

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Mapping the landscape of synthetic lethal interactions in liver cancer.

Theranostics 2021 26;11(18):9038-9053. Epub 2021 Aug 26.

Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou, China.

Almost all the current therapies against liver cancer are based on the "one size fits all" principle and offer only limited survival benefit. Fortunately, synthetic lethality (SL) may provide an alternate route towards individualized therapy in liver cancer. The concept that simultaneous losses of two genes are lethal to a cell while a single loss is non-lethal can be utilized to selectively eliminate tumors with genetic aberrations. Read More

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Chimeric antigen receptor-T cells immunotherapy for targeting breast cancer.

Res Pharm Sci 2021 Oct 19;16(5):447-454. Epub 2021 Aug 19.

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

Redirected chimeric antigen receptor (CAR) T-cells can recognize and eradicate cancer cells in a major histocompatibility complex independent manner. Genetic engineering of T cells through CAR expression has yielded great results in the treatment of hematological malignancies compared with solid tumors. There has been a constant effort to enhance the effectiveness of these living drugs, due to their limited success in targeting solid tumors. Read More

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October 2021

Knowing When to Use Stereotactic Ablative Radiation Therapy in Oligometastatic Cancer.

Cancer Manag Res 2021 7;13:7009-7031. Epub 2021 Sep 7.

Department of Radiotherapy and Radiosurgery, IRCCS Humanitas Research Hospital, Milan, Italy.

Oligometastatic patients are a heterogeneous and yet not well-defined population. The actual definition identifies as oligometastatic, patients with 1-5 metastases in 1-3 different organs. However, only a proportion of these patients are "true" oligometastatic and therefore derive some kinds of benefit from local ablative approaches like stereotactic ablative radiation therapy (SABR). Read More

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September 2021

Phoniatric, Audiological, Orodental and Speech Problems in a Boy with Cardio-Facio-Cutaneous Syndrome Type 3 (CFC 3) Due to a Pathogenic Variant in - Case Study.

Appl Clin Genet 2021 6;14:389-398. Epub 2021 Sep 6.

Department of Clinical Genetics, Medical University of Białystok, Białystok, Poland.

Cardio-facio-cutaneous syndrome 3 (CFC3) due to variants in is a rare genetic disorder manifested mainly by short stature, facial dysmorphism, abnormalities of the cardiovascular system, skin changes, and intellectual disability. The aim of the study is the evaluation of the occurrence of pathological changes in the upper respiratory tract, orthodontic disorders, as well as voice, speech and hearing abnormalities in an 11-year-old boy with CFC3 syndrome. The lack of detailed diagnostics of speech, voice and hearing disorders, as well as the degree of their severity was an inspiration to undertake research in this field. Read More

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September 2021

Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii.

Sci Rep 2021 Sep 14;11(1):18270. Epub 2021 Sep 14.

Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo, 204-8588, Japan.

Trichosporon asahii is a pathogenic fungus that causes severe, deep-seated fungal infections in neutropenic patients. Elucidating the infection mechanisms of T. asahii based on genetic studies requires a specific gene-targeting system. Read More

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September 2021

Therapeutic targeting of the inflammasome in myeloid malignancies.

Blood Cancer J 2021 Sep 14;11(9):152. Epub 2021 Sep 14.

Division of Hemato-Oncology, Department of Oncology, Albert Einstein College of Medicine, Bronx, NY, USA.

Even though genetic perturbations and mutations are important for the development of myeloid malignancies, the effects of an inflammatory microenvironment are a critical modulator of carcinogenesis. Activation of the innate immune system through various ligands and signaling pathways is an important driver of myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). The DAMPs, or alarmins, which activate the inflammasome pathway via the TLR4/NLR signaling cascade causes the lytic cell death of hematopoietic stem and progenitor cells (HSPCs), ineffective hematopoiesis, and β-catenin-induced proliferation of cancer cells, leading to the development of MDS/AML phenotype. Read More

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September 2021

A Randomized Phase II Trial of Idiotype Vaccination and Adoptive Autologous T-Cell Transfer in Multiple Myeloma patients.

Blood 2021 Sep 14. Epub 2021 Sep 14.

City of Hope, Duarte, California, United States.

We hypothesized that combining adoptively transferred autologous T cells with a cancer vaccine strategy would enhance therapeutic efficacy by adding anti-myeloma idiotype-keyhole limpet hemocyanin (Id-KLH) vaccine to vaccine-specific co-stimulated T cells. In this randomized, phase II trial, eligible patients received either the control (KLH only) or Id-KLH vaccine, an auto-transplant, vaccine-specific co-stimulated T-cells expanded ex-vivo, and two booster doses of the assigned vaccine. In 36 patients (20 in KLH, 16 in Id-KLH) enrolled, no dose-limiting toxicity was seen in either arm. Read More

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September 2021

Assessment of Long-Term in vitro Multiplied Human Wharton's Jelly-Derived Mesenchymal Stem Cells prior to Their Use in Clinical Administration.

Cells Tissues Organs 2021 Sep 14:1-11. Epub 2021 Sep 14.

Surat Raktadan Kendra and Research Centre, Surat, India.

The quantity of mesenchymal stem/stromal cells (MSCs) required for a particular therapy demands their subsequent expansion through ex vivo culture. During in vitro multiplication, they undergo replicative senescence which may alter their genetic stability. Therefore, this study was aimed to analyze cellular, molecular, and chromosomal alterations in Wharton's jelly-derived MSCs (WJ-MSCs) during their in vitro sequential passages, where WJ-MSCs were sequentially passaged up to P14 and cells were evaluated at an interval of P2, P6, P10, and P14. Read More

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September 2021

A Case of a 49-Year-Old Man with Nonclassical Fabry Disease Diagnosed by Renal Biopsy.

Nephron 2021 Sep 14:1-4. Epub 2021 Sep 14.

Department of Nephrology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Hangzhou, China.

Fabry disease (FD) is a rare X-linked lysosomal storage disorder caused by mutations in the galactosidase A (GLA) gene that result in deficiency of α-GLA activity, leading to major organ failure and premature mortality. According to different disease courses, FD can be divided into classical and nonclassical phenotypes. The nonclassical FD phenotype is always absent of characteristic symptoms, which makes identifying it challenging. Read More

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September 2021

Local ablative treatment with surgery and/or radiotherapy in single-site and oligometastatic carcinoma of unknown primary.

Eur J Cancer 2021 Sep 11;157:179-189. Epub 2021 Sep 11.

Clinical Cooperation Unit Molecular Hematology/Oncology, German Cancer Research Center (DKFZ), Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany; Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany; Department of Medical Oncology, National Center for Tumor Diseases (NCT), University of Heidelberg, Heidelberg, Germany. Electronic address:

Background: Single-site carcinoma of unknown primary (CUP) is recognised as a distinct favourable subtype in the European Society of Medical Oncology (ESMO) classification. There is broad consensus that these patients are candidates for local ablative treatment strategies with surgery and/or radiotherapy, but data on their outcomes are scarce.

Patients And Methods: In this study, we have addressed the prospects of cure and prognostic factors in a retrospective cohort of 63 patients who were eligible for local treatment at our centre. Read More

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September 2021

First mitogenome of moniezia sichuanensis from forest musk deer with comparative analyses within cyclophyllidea.

Vet Parasitol 2021 Sep 8;299:109575. Epub 2021 Sep 8.

Key Laboratory of Non-Invasive Research Technology for Endangered Species, School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, 10083, China; Department of Zoology, School of Ecology and Nature Conservation, Beijing Forestry University, 35 Qinghua East Road, Beijing, 100083, China. Electronic address:

We characterised, for the first time, the whole mitogenome of a unique tapeworm, Moniezia sichuanensis (Cyclophyllidea, Anoplocephalidae) of forest musk deer (Moschus berezovskii). The total length of the circular mitogenome was 13,652 bp. It consisted of 12 protein-coding genes (PCGs), 22 transfer RNA genes, and two ribosomal RNA genes, which are typical of the mitogenomes of Moniezia. Read More

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September 2021

Long non-coding RNAs as new players in bladder cancer: Lessons from pre-clinical and clinical studies.

Life Sci 2021 Sep 11:119948. Epub 2021 Sep 11.

Department of Food Hygiene and Quality Control, Division of Epidemiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

The clinical management of bladder cancer (BC) has become an increasing challenge due to high incidence rate of BC, malignant behavior of cancer cells and drug resistance. The non-coding RNAs are considered as key factors involved in BC progression. The long non-coding RNAs (lncRNAs) are RNA molecules and do not encode proteins. Read More

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September 2021

Black box of phage-bacterium interactions: exploring alternative phage infection strategies.

Open Biol 2021 Sep 15;11(9):210188. Epub 2021 Sep 15.

Department of Microbiology and Molecular Genetics, University of California, One Shields Avenue, Davis, CA 95616, USA.

The canonical lytic-lysogenic binary has been challenged in recent years, as more evidence has emerged on alternative bacteriophage infection strategies. These infection modes are little studied, and yet they appear to be more abundant and ubiquitous in nature than previously recognized, and can play a significant role in the ecology and evolution of their bacterial hosts. In this review, we discuss the extent, causes and consequences of alternative phage lifestyles, and clarify conceptual and terminological confusion to facilitate research progress. Read More

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September 2021

Synthesis of human amyloid restricted to liver results in an Alzheimer disease-like neurodegenerative phenotype.

PLoS Biol 2021 Sep 14;19(9):e3001358. Epub 2021 Sep 14.

Curtin Health Innovation Research Institute, Faculty of Health Sciences, Curtin University, Bentley, Australia.

Several lines of study suggest that peripheral metabolism of amyloid beta (Aß) is associated with risk for Alzheimer disease (AD). In blood, greater than 90% of Aß is complexed as an apolipoprotein, raising the possibility of a lipoprotein-mediated axis for AD risk. In this study, we report that genetic modification of C57BL/6J mice engineered to synthesise human Aß only in liver (hepatocyte-specific human amyloid (HSHA) strain) has marked neurodegeneration concomitant with capillary dysfunction, parenchymal extravasation of lipoprotein-Aß, and neurovascular inflammation. Read More

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September 2021

Identification of hub genes in common cancers of women in India and targeting for the search of anticancer agent from phytoconstituent using interaction network analysis and virtual screening.

J Biomol Struct Dyn 2021 Sep 14:1-7. Epub 2021 Sep 14.

Department of Applied Science, Indian Institute of Information Technology Allahabad, Prayagraj, India.

Cancer is one of the most dreadful diseases across the globe, with the advancement in this field a great advent has been achieved in treating cancer by various therapies like chemotherapy, radiation therapy, hormone therapy, gene therapy, and many more but also the most serious concern associated with the available treatments are the toxicities or the side effects linked to them, apart from this the treatment of many malignancies are still not available, because of these such issues, tremendous research is still going on in the whole world to find a better and more potent treatment option for cancer. Cancer develops due to the synergistic effects of both genetic and epigenetic factors. The mutations that change the normal functioning of the genes are responsible for cancer. Read More

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September 2021

The Complex Transcriptional Landscape of Magnetosome Gene Clusters in Magnetospirillum gryphiswaldense.

mSystems 2021 Sep 14:e0089321. Epub 2021 Sep 14.

Department of Microbiology, University of Bayreuthgrid.7384.8, Bayreuth, Germany.

Magnetosomes are complex membrane organelles synthesized by magnetotactic bacteria (MTB) for navigation in the Earth's magnetic field. In the alphaproteobacterium Magnetospirillum gryphiswaldense, all steps of magnetosome formation are tightly controlled by >30 specific genes arranged in several gene clusters. However, the transcriptional organization of the magnetosome gene clusters has remained poorly understood. Read More

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September 2021