3,604 results match your criteria gene nidulans


Deletion of Aspergillus nidulans cpsA/rseA induces increased extracellular hydrolase production in solid-state culture partly through the high osmolarity glycerol pathway.

J Biosci Bioeng 2021 Apr 4. Epub 2021 Apr 4.

Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address:

Koji molds, such as Aspergillus oryzae and Aspergillus sojae, are used in the food industry in East Asia and have been explored for the large-scale production of extracellular hydrolases. We previously found that the deletion of a gene encoding a putative GT2 glycosyltransferase increased production of extracellular hydrolases in A. sojae. Read More

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Function of pH-dependent transcription factor PacC in regulating development, pathogenicity, and mycotoxin biosynthesis of phytopathogenic fungi.

FEBS J 2021 Mar 9. Epub 2021 Mar 9.

Key Laboratory of Plant Resources, Institute of Botany, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.

pH, as one of the most important environmental factors, affects various biological processes in pathogenic fungi. Sensing and responding to fluctuations in ambient pH are essential for these fungi to complete their life cycle. Fungi have evolved a complicated and conserved system, the so-called Pal-pH pathway, to regulate genes and adapt to alterations in ambient pH. Read More

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Artificial AmyR::XlnR transcription factor induces α-amylase production in response to non-edible xylan-containing hemicellulosic biomass.

Enzyme Microb Technol 2021 Apr 12;145:109762. Epub 2021 Feb 12.

Faculty of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi, 468-8502, Japan. Electronic address:

Filamentous fungi belonging to the Aspergillus genus are one of the most favored microorganisms for industrial enzyme production because they can secrete large amounts of proteins into the culture medium. α-Amylase, an enzyme produced by Aspergillus species, is important for food and industrial applications. The production of α-amylase is induced by starch, mainly obtained from the edible biomass; however, the increasing demand for foods is limiting the application of the latter. Read More

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Functions of PUF family RNA-binding proteins in .

J Microbiol Biotechnol 2021 Mar 12. Epub 2021 Mar 12.

School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea.

RNA-binding proteins are involved in RNA metabolism and posttranscriptional regulation of various fundamental biological processes. The PUF family of RNA-binding proteins is highly conserved in eukaryotes, and its members regulate gene expression, mitochondrial biogenesis, and RNA processing. However, their biological functions in species remain mostly unknown in filamentous fungi. Read More

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Mannitol-1-phosphate dehydrogenase, MpdA, is required for mannitol production in vegetative cells and involved in hyphal branching, heat resistance of conidia and sexual development in Aspergillus nidulans.

Curr Genet 2021 Mar 8. Epub 2021 Mar 8.

Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, 34134, South Korea.

Aspergillus nidulans produces cleistothecia as sexual reproductive organs in a process affected by genetic and external factors. To gain a deeper insight into A. nidulans sexual development, we performed comparative proteome analyses based on the wild type developmental periods. Read More

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DUG pathway governs degradation of intracellular glutathione in .

Appl Environ Microbiol 2021 Feb 26. Epub 2021 Feb 26.

Department of Molecular Biotechnology and Microbiology, Faculty of Sciences and Technology, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary.

Glutathione (GSH) is an abundant tripeptide that plays a crucial role in shielding cellular macromolecules from various reactive oxygen and nitrogen species in fungi. Understanding GSH metabolism is of vital importance to deciphering redox regulation in these microorganisms. In the present study, to better understand the GSH metabolism in filamentous fungi, we investigate functions of the and genes in the model fungus These genes are orthologues of involved in cytosolic GSH degradation in the yeast. Read More

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February 2021

Identification of the genes encoding the catalytic steps corresponding to LRA4 (l-2-keto-3-deoxyrhamnonate aldolase) and l-lactaldehyde dehydrogenase in Aspergillus nidulans: evidence for involvement of the loci AN9425/lraD and AN0544/aldA in the l-rhamnose catabolic pathway.

Environ Microbiol 2021 Feb 21. Epub 2021 Feb 21.

Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), c/ Catedrático Agustín Escardino Benlloch 7, Paterna, Valencia, 46980, Spain.

l-rhamnose is found in nature mainly as a component of structural plant polysaccharides and can be used as a carbon source by certain microorganisms. Catabolism of this sugar in bacteria, archaea and fungi occurs by two routes involving either phosphorylated or non-phosphorylated intermediates. Unlike the corresponding pathway in yeasts, the metabolic details of the non-phosphorylated pathway in filamentous fungi are not fully defined. Read More

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February 2021

Genome mining of cryptic tetronate natural products from a PKS-NRPS encoding gene cluster in t-22.

Org Biomol Chem 2021 03;19(9):1985-1990

Department of Chemical and Biomolecular Engineering, University of California Los Angeles, CA 90095, USA.

Trichoderma harzianum is a widely used biocontrol agent in agriculture. Obtaining a full inventory of the small molecules that can be biosynthesized from the encoded biosynthetic gene clusters (BGCs) is therefore useful for understanding associated plant-microbe and microbe-microbe interactions. Here we heterologously reconstituted a polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) encoding gene cluster from T. Read More

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Transcriptomic, Protein-DNA Interaction, and Metabolomic Studies of VosA, VelB, and WetA in Aspergillus nidulans Asexual Spores.

mBio 2021 02 9;12(1). Epub 2021 Feb 9.

School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea

In filamentous fungi, asexual development involves cellular differentiation and metabolic remodeling leading to the formation of intact asexual spores. The development of asexual spores (conidia) in is precisely coordinated by multiple transcription factors (TFs), including VosA, VelB, and WetA. Notably, these three TFs are essential for the structural and metabolic integrity, i. Read More

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February 2021

Twists and Turns in the Salicylate Catabolism of , Revealing New Roles of the 3-Hydroxyanthranilate Pathway.

mSystems 2021 Jan 26;6(1). Epub 2021 Jan 26.

Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal

In fungi, salicylate catabolism was believed to proceed only through the catechol branch of the 3-oxoadipate pathway, as shown, e.g., in However, the observation of a transient accumulation of gentisate upon the cultivation of in salicylate medium questions this concept. Read More

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January 2021

Genetic Transformation in Species.

Authors:
Ping Wang

J Fungi (Basel) 2021 Jan 15;7(1). Epub 2021 Jan 15.

Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA.

Genetic transformation plays an imperative role in our understanding of the biology in unicellular yeasts and filamentous fungi, such as , and . It also helps to understand the virulence and drug resistance mechanisms of the pathogenic fungus that causes cryptococcosis in health and immunocompromised individuals. Since the first attempt at DNA transformation in this fungus by Edman in 1992, various methods and techniques have been developed to introduce DNA into this organism and improve the efficiency of homology-mediated gene disruption. Read More

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January 2021

HbxB Is a Key Regulator for Stress Response and β-Glucan Biogenesis in .

Microorganisms 2021 Jan 11;9(1). Epub 2021 Jan 11.

School of Food Science and Biotechnology Kyungpook National University, Daegu 41566, Korea.

Homeobox transcription factors are conserved in eukaryotes and act as multi-functional transcription factors in filamentous fungi. Previously, it was demonstrated that HbxB governs fungal development and spore viability in . Here, the role of HbxB in was further characterized. Read More

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January 2021

A novel mycovirus infecting Aspergillus nidulans that is closely related to viruses in a new genus of the family Partitiviridae.

Arch Virol 2021 Feb 6;166(2):659-664. Epub 2021 Jan 6.

School of Environmental Sciences, University of Guelph, Guelph, ON, N1G 2W1, Canada.

The bisegmented genome of a novel double-stranded (ds) RNA mycovirus, named "Aspergillus nidulans partitivirus 1" (AnPV1), isolated from the fungus Aspergillus nidulans strain HJ5-47, was sequenced and analyzed. AnPV1 contains two segments, AnPV1-1 and AnPV1-2. AnPV1-1 has 1837 bp with an open reading frame (ORF) that potentially encodes a putative RNA-dependent RNA polymerase (RdRp) of 572 amino acids (aa). Read More

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February 2021

Carbon Catabolite Repression in Filamentous Fungi Is Regulated by Phosphorylation of the Transcription Factor CreA.

mBio 2021 01 5;12(1). Epub 2021 Jan 5.

Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirao Preto, Brazil

Filamentous fungi of the genus are of particular interest for biotechnological applications due to their natural capacity to secrete carbohydrate-active enzymes (CAZy) that target plant biomass. The presence of easily metabolizable sugars such as glucose, whose concentrations increase during plant biomass hydrolysis, results in the repression of CAZy-encoding genes in a process known as carbon catabolite repression (CCR), which is undesired for the purpose of large-scale enzyme production. To date, the CH transcription factor CreA has been described as the major CC repressor in spp. Read More

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January 2021

Novel Biological Functions of the NsdC Transcription Factor in Aspergillus fumigatus.

mBio 2021 01 5;12(1). Epub 2021 Jan 5.

Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

The fungal zinc finger transcription factor NsdC is named after, and is best known for, its essential role in sexual reproduction (ever in exual evelopment). In previous studies with , it was also shown to have roles in promotion of vegetative growth and suppression of asexual conidiation. In this study, the function of the homologue in the opportunistic human pathogen was investigated. Read More

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January 2021

Prenylation and Dehydrogenation of a 2-Reversely Prenylated Diketopiperazine as a Branching Point in the Biosynthesis of Echinulin Family Alkaloids in .

ACS Chem Biol 2021 01 31;16(1):185-192. Epub 2020 Dec 31.

Institut für Pharmazeutische Biologie und Biotechnologie, Fachbereich Pharmazie, Philipps-Universität Marburg, Robert-Koch-Straße 4, Marburg 35037, Germany.

The echinulin family alkaloids can be grouped into three series depending on the number of the double bonds adjacent to the diketopiperazine core structure. Heterologous expression of the putative echinulin biosynthetic gene cluster from in led to accumulation of echinulin without a double bond and neoechinulin A with one double bond (Δ) as major products. Their analogues with a different number of prenyl moieties were detected as minor products. Read More

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January 2021

An Platform for the Complete Cluster Refactoring and Total Biosynthesis of Fungal Natural Products.

ACS Synth Biol 2021 01 29;10(1):173-182. Epub 2020 Dec 29.

Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, California 90089, United States.

Fungal natural products (NPs) comprise a vast number of bioactive molecules with diverse activities, and among them are many important drugs. However, the yields of fungal NPs from native producers are usually low, and total synthesis of structurally complex NPs is challenging. As such, downstream derivatization and optimization of lead fungal NPs can be impeded by the high cost of obtaining sufficient starting material. Read More

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January 2021

Heteroexpression of in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes.

Mar Drugs 2020 Dec 18;18(12). Epub 2020 Dec 18.

Ocean College, Zhejiang University, Zhoushan 316021, China.

Fungi are a prospective resource of bioactive compounds, but conventional methods of drug discovery are not effective enough to fully explore their metabolic potential. This study aimed to develop an easily attainable method to elicit the metabolic potential of fungi using as a transcription regulation tool. In this study, functional analysis of (AnLaeA) and sp. Read More

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December 2020

Hancockiamides: phenylpropanoid piperazines from are biosynthesised by a versatile dual single-module NRPS pathway.

Org Biomol Chem 2021 01;19(3):587-595

School of Molecular Sciences, The University of Western Australia, Perth, WA 6009, Australia.

The hancockiamides are an unusual new family of N-cinnamoylated piperazines from the Australian soil fungus Aspergillus hancockii. Genomic analyses of A. hancockii identified a biosynthetic gene cluster (hkm) of 12 genes, including two single-module nonribosomal peptide synthetase (NRPS) genes. Read More

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January 2021

Rice-induced secondary metabolite gene expression in Aspergillus nidulans.

J Ind Microbiol Biotechnol 2020 Dec 18;47(12):1109-1116. Epub 2020 Nov 18.

Department of Medicine, School of Medicine and Public Health, University of Wisconsin Madison, Madison, WI, 53706, USA.

Activation of silent biosynthetic gene clusters in fungi remains a challenge for discovery of new useful natural products. In this work, we identify a group of silent secondary metabolite gene clusters in Aspergillus nidulans that are induced by rice (Oryza sativa). Using reverse phase HPLC purification on extracts of rice, we identified the plant hormone gibberellic acid as one compound present in rice extracts that induced these silent genes. Read More

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December 2020

The Conserved MAP Kinase MpkB Regulates Development and Sporulation without Affecting Aflatoxin Biosynthesis in .

J Fungi (Basel) 2020 Nov 16;6(4). Epub 2020 Nov 16.

Department of Pharmaceutical Engineering, Woosuk University, Wanju 55338, Korea.

In eukaryotes, the MAP kinase signaling pathway plays pivotal roles in regulating the expression of genes required for growth, development, and stress response. Here, we deleted the gene (AFLA_034170), an ortholog of the gene, to characterize its function in , a cosmopolitan, pathogenic, and aflatoxin-producing fungus. Previous studies revealed that MpkB positively regulates sexual and asexual differentiation in . Read More

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November 2020

Post-Synthetic Reduction of Pectin Methylesterification Causes Morphological Abnormalities and Alterations to Stress Response in .

Plants (Basel) 2020 Nov 12;9(11). Epub 2020 Nov 12.

Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.

Pectin is a critical component of the plant cell wall, supporting wall biomechanics and contributing to cell wall signaling in response to stress. The plant cell carefully regulates pectin methylesterification with endogenous pectin methylesterases (PMEs) and their inhibitors (PMEIs) to promote growth and protect against pathogens. We expressed pectin methylesterase (AnPME) in plants to determine the impacts of methylesterification status on pectin function. Read More

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November 2020

Type II phosphatidylserine decarboxylase is crucial for the growth and morphogenesis of the filamentous fungus Aspergillus nidulans.

J Biosci Bioeng 2021 Feb 24;131(2):139-146. Epub 2020 Oct 24.

Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, 113-8657 Tokyo, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address:

Phosphatidylserine decarboxylases (PSDs) catalyze the production of phosphatidylethanolamine (PE) from phosphatidylserine (PS) and are crucial for the maintenance of PE levels in fungi. The PSDs are classified into two types; the type I PSDs are conserved from bacteria to humans, while the type II PSDs exist only in fungi and plants. In yeasts, the deletion of type I PSD-encoding genes causes severe growth retardation. Read More

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February 2021

Internalization of the AstA Transporter into Mitochondria Depends on Growth Conditions, and Affects ATP Levels and Sulfite Oxidase Activity.

Int J Mol Sci 2020 Oct 19;21(20). Epub 2020 Oct 19.

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A str., 02-106 Warsaw, Poland.

The gene encoding an alternative sulfate transporter was originally cloned from the genome of the Japanese isolate as a suppressor of sulfate permease-deficient strains. Expression of the gene is under the control of the sulfur metabolite repression system. The encoded protein transports sulfate across the cell membrane. Read More

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October 2020

In the fungus where it happens: History and future propelling Aspergillus nidulans as the archetype of natural products research.

Fungal Genet Biol 2020 Nov 6;144:103477. Epub 2020 Oct 6.

Department of Medical Microbiology and Immunology, University of Wisconsin- Madison, Madison, WI, United States; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States. Electronic address:

In 1990 the first fungal secondary metabolite biosynthetic gene was cloned in Aspergillus nidulans. Thirty years later, >30 biosynthetic gene clusters (BGCs) have been linked to specific natural products in this one fungal species. While impressive, over half of the BGCs in A. Read More

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November 2020

Identification and characterization of β-d-galactofuranosidases from Aspergillus nidulans and Aspergillus fumigatus.

J Biosci Bioeng 2021 Jan 1;131(1):1-7. Epub 2020 Oct 1.

Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address:

Although β-d-galactofuranosidases (Galf-ases) that hydrolyze β-d-galactofuranose (Galf)-containing oligosaccharides have been characterized in various organisms, to date no Galf-specific Galf-ase-encoding genes have been reported in Aspergillus fungi. Based on the amino acid sequences of previously identified bacterial Galf-ases, here we found two candidate Galf-specific Galf-ase genes AN2395 (gfgA) and AN3200 (gfgB) in the genome of Aspergillus nidulans. Indeed, recombinant GfgA and GfgB proteins exhibited Galf-specific Galf-ase activity, but no detectable α-l-arabinofuranosidase (Araf-ase) activity. Read More

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January 2021

Catabolism of L-rhamnose in A. nidulans proceeds via the non-phosphorylated pathway and is glucose repressed by a CreA-independent mechanism.

Microb Cell Fact 2020 Oct 2;19(1):188. Epub 2020 Oct 2.

Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), c/Catedrático Agustín Escardino Benlloch 7, 46980, Paterna, Valencia, Spain.

L-rhamnose (6-deoxy-mannose) occurs in nature mainly as a component of certain plant structural polysaccharides and bioactive metabolites but has also been found in some microorganisms and animals. The release of L-rhamnose from these substrates is catalysed by extracellular enzymes including α-L-rhamnosidases, the production of which is induced in its presence. The free sugar enters cells via specific uptake systems where it can be metabolized. Read More

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October 2020

A novel fungal gene regulation system based on inducible VPR-dCas9 and nucleosome map-guided sgRNA positioning.

Appl Microbiol Biotechnol 2020 Nov 2;104(22):9801-9822. Epub 2020 Oct 2.

Fungal Genetics Lab, Institute of Microbial Genetics, Department of Applied Genetics and Cell Biology, BOKU-University of Natural Resources and Life Sciences Vienna, BOKU-Campus Tulln, Konrad Lorenz Strasse 24, A-3430, Tulln an der Donau, Austria.

Programmable transcriptional regulation is a powerful tool to study gene functions. Current methods to selectively regulate target genes are mainly based on promoter exchange or on overexpressing transcriptional activators. To expand the discovery toolbox, we designed a dCas9-based RNA-guided synthetic transcription activation system for Aspergillus nidulans that uses enzymatically disabled "dead" Cas9 fused to three consecutive activation domains (VPR-dCas9). Read More

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November 2020

On the use of n-octyl gallate and salicylhydroxamic acid to study the alternative oxidase role.

Arch Biochem Biophys 2020 11 25;694:108603. Epub 2020 Sep 25.

Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Copilco, Cd. Universitaria, Coyoacán, 04510, Ciudad de México, Mexico. Electronic address:

The alternative oxidase (AOX) catalyzes the transfer of electrons from ubiquinol to oxygen without the translocation of protons across the inner mitochondrial membrane. This enzyme has been proposed to participate in the regulation of cell growth, sporulation, yeast-mycelium transition, resistance to reactive oxygen species, infection, and production of secondary metabolites. Two approaches have been used to evaluate AOX function: incubation of cells for long periods of time with AOX inhibitors or deletion of AOX gene. Read More

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November 2020

Velvet activated McrA plays a key role in cellular and metabolic development in Aspergillus nidulans.

Sci Rep 2020 09 15;10(1):15075. Epub 2020 Sep 15.

Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, 53706, USA.

McrA is a key transcription factor that functions as a global repressor of fungal secondary metabolism in Aspergillus species. Here, we report that mcrA is one of the VosA-VelB target genes and McrA governs the cellular and metabolic development in Aspergillus nidulans. The deletion of mcrA resulted in a reduced number of conidia and decreased mRNA levels of brlA, the key asexual developmental activator. Read More

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September 2020