133,059 results match your criteria gel-permeation chromatography


Isolation, characterization, and antimicrobial activity of communic acid from .

J Complement Integr Med 2021 Jul 29. Epub 2021 Jul 29.

Laboratory of Organic Synthesis, Extraction and Valorisation, Faculty of Sciences Aïn Chock, Hassan II University of Casablanca, BP. 20000, Casablanca, Morocco.

Objectives: A mixture of Z and E communic acid is isolated for the first time from the cones of Its biological activity was studied.

Methods: The plant material was extracted in a Soxhlet apparatus with -hexane, the resulting extract was subjected to column chromatography (CC) on silica gel. The structure elucidation of the constituents of the isolated fraction was identified by comparison of its spectroscopic properties H and C NMR data with those reported in the literature. Read More

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Yeast Lipid Extraction and Analysis by HPTLC.

Bio Protoc 2021 Jul 5;11(13):e4081. Epub 2021 Jul 5.

State Key Laboratory of Microbial Metabolism & Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

The diversity of lipid structures, properties, and combinations in biological tissues makes their extraction and analysis an experimental challenge. Accordingly, even for one of the simplest single-cellular fungi, the budding yeast (), numerous extraction and analysis protocols have been developed to separate and quantitate the different molecular lipid species. Among them, most are quite sophisticated and tricky to follow. Read More

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Tear Film Proteome of Healthy Domestic Cats.

Vet Med Int 2021 15;2021:8708023. Epub 2021 Jul 15.

Department of Agricultural and Environmental Sciences, Santa Cruz State University, Rodovia Jorge Amado, km 16, Salobrinho, Ilhéus, Bahia, Brazil.

The aim of this study was to investigate the proteins found in tear film of healthy domestic cats. Schirmer tear test strips were used to collect tear samples of twelve healthy cats, which were mixed, centrifuged, and placed in a single 1.5 mL microtube that was frozen at -20°C, until analysis by two-dimensional polyacrylamide gel and mass spectrometry associated with high-performance liquid chromatography. Read More

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Characterisation of the Mouse Cerebellar Proteome in the GFAP-IL6 Model of Chronic Neuroinflammation.

Cerebellum 2021 Jul 29. Epub 2021 Jul 29.

School of Medicine, Western Sydney University, Locked Bag 1797, Penrith, NSW, 2751, Australia.

GFAP-IL6 transgenic mice are characterised by astroglial and microglial activation predominantly in the cerebellum, hallmarks of many neuroinflammatory conditions. However, information available regarding the proteome profile associated with IL-6 overexpression in the mouse brain is limited. This study investigated the cerebellum proteome using a top-down proteomics approach using 2-dimensional gel electrophoresis followed by liquid chromatography-coupled tandem mass spectrometry and correlated these data with motor deficits using the elevated beam walking and accelerod tests. Read More

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Microplastics in the environment: Sampling, pretreatment, analysis and occurrence based on current and newly-exploited chromatographic approaches.

Sci Total Environ 2021 Jun 29;794:148725. Epub 2021 Jun 29.

Laboratory of Environmental Pollution Control, Department of Chemistry, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Greece; Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Balkan Center, Thessaloniki GR-57001, Greece. Electronic address:

The omnipresent character of microplastics (MPs) in environmental matrices, organisms and products has recently posed the need of their qualitative as well as quantitative analysis imperative, in order to provide data about their abundance and specification of polymer types in several substrates. In this framework, current and emerging approaches based on the chromatographic separation are of increased relevance in the field of MPs analysis and possess a large number of merits, since most of them are applicable in various complex matrices, sensitive and ideal for the detection of small-sized particles, whereas the common absence of any special pre-treatment step before analysis should also be highlighted. Αnalytical pyrolysis coupled with gas chromatography mass spectrometry (GC-MS) has recently gained ground as a powerful means to deliver information on MPs composition and degradation after their release into environment. Read More

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Microchip Electrophoresis Assay for Calmodulin Binding Proteins.

J Sep Sci 2021 Feb 7;44(4):895-902. Epub 2020 Dec 7.

Department of Chemistry, University of Kansas, Lawrence, Kansas, USA.

The calcium signaling protein calmodulin regulates numerous intracellular processes. We introduce a sensitive microchip assay to separate and detect calmodulin binding proteins. The assay utilizes an optimized microchip electrophoresis protein separation platform with laser-induced fluorescence detection. Read More

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February 2021

Comparative hydrolysis analysis of cellulose samples and aspects of its application in conservation science.

Cellulose (Lond) 2021 Jul 23:1-16. Epub 2021 Jul 23.

Department of Chemistry, Institute of Chemistry of Renewables, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, 1190 Austria.

Knowledge about the carbohydrate composition of pulp and paper samples is essential for their characterization, further processing, and understanding the properties. In this study, we compare sulfuric acid hydrolysis and acidic methanolysis, followed by GC-MS analysis of the corresponding products, by means of 42 cellulose and polysaccharide samples. Results are discussed and compared to solid-state NMR (crystallinity) and gel permeation chromatography (weight-averaged molecular mass) data. Read More

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Tailored nanodisc immobilization for one-step purification and reconstitution of cytochrome P450: A tool for membrane proteins' hard cases.

J Sep Sci 2021 Jul 26. Epub 2021 Jul 26.

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.

A novel nanodisc-based immobilization method was developed for high-efficient purification and reconstitution of cytochrome P450 in one step. Using membrane scaffold protein containing a histidine tag, charged-nanodiscs were prepared in the form of self-assembly of lipid-protein nanoparticles. Their properties including the particle diameter and its distribution, and Zeta potential were controlled well by adjusting molar ratios of phospholipids to membrane scaffold protein. Read More

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Snake venom proteomics and antivenomics of two Sundaic lance-headed pit vipers: Trimeresurus wiroti (Malaysia) and Trimeresurus puniceus (Indonesia).

Comp Biochem Physiol Part D Genomics Proteomics 2021 Jul 5;40:100875. Epub 2021 Jul 5.

Venom Research & Toxicology Laboratory, Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. Electronic address:

Envenomation by two medically important Sundaic pit vipers, Trimeresurus wiroti (Malaysia) and Trimeresurus puniceus (Indonesia), causes hemotoxic syndrome with a potentially fatal outcome. Research on the compositions and antigenicity of these pit viper venoms is however lacking, limiting our understanding of the pathophysiology and treatment of envenomation. This study investigated the venom proteomes of both species through a protein decomplexation strategy, applying C reverse-phase high-performance liquid chromatography followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and protein identification through nano-electrospray ionization liquid chromatography-tandem mass spectrometry (nano-ESI-LCMS/MS) of trypsin-digested peptides. Read More

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Depolymerization of biorefinery lignin by improved laccases of the white-rot fungus Obba rivulosa.

Microb Biotechnol 2021 Jul 26. Epub 2021 Jul 26.

Fungal Genetics and Biotechnology, Department of Microbiology, University of Helsinki, University of Helsinki, Biocenter 1 PO Box 56 Viikinkaari 9, Helsinki, FI-00014, Finland.

Fungal laccases are attracting enzymes for sustainable valorization of biorefinery lignins. To improve the lignin oxidation capacity of two previously characterized laccase isoenzymes from the white-rot fungus Obba rivulosa, we mutated their substrate-binding site at T1. As a result, the pH optimum of the recombinantly produced laccase variant rOrLcc2-D206N shifted by three units towards neutral pH. Read More

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Stability of Direct Oral Anticoagulants and Antiarrhythmic Drugs in Serum Collected in Standard (Non-Gel) Serum Tubes versus Tubes Containing Gel Separators.

Ther Drug Monit 2021 Jul 20. Epub 2021 Jul 20.

Department of Clinical Pharmacology, St. Olav University Hospital, Trondheim, Norway. Department of Laboratory Medicine, University Hospital of North Norway, Tromsø, Norway. Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway.

Background: Separation gels are often used in collection tubes, but adsorption of drugs onto the gel may cause falsely low concentrations in therapeutic drug monitoring. In this study, the stability of apixaban, edoxaban, rivaroxaban, flecainide, amiodarone, and desethylamiodarone was assessed in tubes, with and without gel separators.

Methods: Drug-free blood was spiked and stored for up to 7 d in non-gel tubes and gel tubes from two manufacturers (Vacuette and Vacutainer). Read More

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Systematic process evaluation of the conjugation of proteins to gold nanoparticles.

Heliyon 2021 Jun 25;7(6):e07392. Epub 2021 Jun 25.

Laboratorio de Biomateriales, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, 11400, Montevideo, Uruguay.

The present work addresses some fundamental aspects in the preparation of protein-conjugated gold nanoparticles, in order to ensure an appropriate final product. Ten broadly available and/or easy to implement analytical tools were benchmarked and compared in their capacity to provide reliable and conclusive information for each step of the procedure. These techniques included transmission electron microscopy, UV/VIS spectroscopy, dynamic light scattering, zeta-potential, Fourier-transformed infrared spectroscopy, colloidal stability titration, end-point colloidal stability analysis, cyclic voltammetry, agarose gel electrophoresis and size-exclusion chromatography (SEC). Read More

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Investigation of the consequences of ultrasound on the physicochemical, emulsification, and gelatinization characteristics of citric acid-treated whey protein isolate.

J Dairy Sci 2021 Jul 22. Epub 2021 Jul 22.

Key Laboratory of Dairy Science, Northeast Agricultural University, Ministry of Education, Harbin, 150030, PR China. Electronic address:

The effect of ultrasound (US) pretreatment (0, 200, 400, 600, and 800 W) on the physicochemical, emulsification, and gelatinization characteristics of citric acid (CA)-treated whey protein isolate (WPI) was investigated. Size exclusion chromatography demonstrated that when compared with untreated WPI, US pretreatment promoted production of more molecular polymers in the CA-treated WPI. There was a reduction in particle size of CA-treated WPI with the increase of US power (0-800 W), whereas its free sulfhydryl content, surface hydrophobicity, and intrinsic fluorescence strength increased. Read More

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Novel polysaccharide extracted from Sipunculus nudus inhibits HepG2 tumour growth in vivo by enhancing immune function and inducing tumour cell apoptosis.

J Cell Mol Med 2021 Jul 24. Epub 2021 Jul 24.

Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Zhuhai Key Laboratory of Marine Bioresources and Environment, School of Marine Sciences, Sun Yat-Sen University, Guangzhou, China.

A novel polysaccharide was extracted from Sipunculus nudus (SNP). The molecular weight (MW) of SNP was determined to be 9223 Da by high-performance gel permeation chromatography analyses, and the structure of the SNP repeat units was determined to be →3,4-β-D-GlcpNAC (1→ and →4) -α-D-Glcp (1→ in the ratio of 15:1; →2) -α -D-Galp - (1→ as a side chain; and β-D-Galp-(1→ and α- D-Glcp - (1→ as end groups by GC-MS analysis and NMR assays. The effect of SNP on hepatoma HepG2-bearing mice was analysed to verify its potential in the clinical treatment of liver cancer. Read More

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Dispersive clean-up process based on magnetic graphene oxide nanocomposite for determination of 2-glycerol monopalmitate in olive oil prior to GC-FID and GC-MS analysis.

J Sci Food Agric 2021 Jul 24. Epub 2021 Jul 24.

Food Technology and Agricultural Products Research center, Standard Research Institute (SRI), Karaj, Iran.

Background: Recently, developing methods for better quality control, fraud detection, and analytical study of olive oil are tremendous components. Magnetic graphene oxide material is known for its reusability, high adsorption capability, and stability in food sample preparation. Monopalmitine or 2-glycerol monopalmitate (2-GMP%) is one of the main parameters in quality essay and classification of olive oil, which can be classified as extra virgin ≤0. Read More

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Polysaccharide- and β-Cyclodextrin-Based Chiral Selectors for Enantiomer Resolution: Recent Developments and Applications.

Molecules 2021 Jul 16;26(14). Epub 2021 Jul 16.

Department of Chemistry, Institute of Chemistry of Renewable Resources, University of Natural Resources and Life Sciences, Konrad-Lorenz-Straße 24, Tulln, A-3430 Vienna, Austria.

Polysaccharides, oligosaccharides, and their derivatives, particularly of amylose, cellulose, chitosan, and β-cyclodextrin, are well-known chiral selectors (CSs) of chiral stationary phases (CSPs) in chromatography, because they can separate a wide range of enantiomers. Typically, such CSPs are prepared by physically coating, or chemically immobilizing the polysaccharide and β-cyclodextrin derivatives onto inert silica gel carriers as chromatographic support. Over the past few years, new chiral selectors have been introduced, and progressive methods to prepare CSPs have been exploited. Read More

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Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars.

Int J Mol Sci 2021 Jul 19;22(14). Epub 2021 Jul 19.

National Institute of Agricultural Science, RDA, Jeonju 54874, Korea.

The low-molecular weight glutenin subunit (LMW-GS) composition of wheat () flour has important effects on end-use quality. However, assessing the contributions of each LMW-GS to flour quality remains challenging because of the complex LMW-GS composition and allelic variation among wheat cultivars. Therefore, accurate and reliable determination of LMW-GS alleles in germplasm remains an important challenge for wheat breeding. Read More

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Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells.

Int J Mol Sci 2021 Jul 13;22(14). Epub 2021 Jul 13.

College of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Korea.

Aging is associated with immune disregulation and oxidative stress which lead to inflammation and neurodegenerative diseases. We have tried to identify the anti-neuroinflammatory and anti-inflammatory components of L. The dried flowers of were extracted with 70% EtOH, and the obtained extract was divided into CHCl, EtOAc, -BuOH, and HO fractions. Read More

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Stability of Compounded Topical Nifedipine in Cream, Gel, and Ointment Bases.

Int J Pharm Compd 2021 Jul-Aug;25(4):344-351

Biopharmaceutics and Drug Delivery Lab, College of Pharmacy, Halifax, NS, Canada.

The objective of this study was to investigate the stability of compounded nifedipine cream in gel and ointment formulations dispensed in white plastic and glass amber jars. Extemporaneously compounded nifedipine cream (Glaxal Base), gel (K-Y Jelly), and ointment (Aquaphor) in white plastic and glass amber jars were stored at 4°C, 23°C, and 40°C. We determined potency on days 0, 7, 14, 30, 60, and 90, and subsequently assigned beyond-use-dates based on United States Pharmacopeia recommendations, organoleptic properties, and pH changes. Read More

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Compatibility of Estradiol, Estriol, Estrone, Progesterone, and Testosterone Single Formulation in Fitalite, Versatile, or HRT Supreme Cream Base.

Int J Pharm Compd 2021 Jul-Aug;25(4):336-343

Fagron US, St. Paul, Minnesota.

In this work, we focus on three ready-to-use vehicles: Fitalite, Versatile, and HRT Supreme Cream Base. Fitalite is a natural, light, hydrophilic gel-cream that contains vitamin E and oil bodies from plant sources (phytosomes), providing antioxidant and skinmoisturizing properties. Versatile is a vanishing oil-inwater cream base which retains its consistency with a broad range and high concentrations of active pharmaceutical ingredients, dermaceutical ingredients, and solvents. Read More

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Purification, identification, and molecular mechanism of DPP-IV inhibitory peptides from defatted Antarctic krill powder.

J Food Biochem 2021 Jul 23:e13872. Epub 2021 Jul 23.

College of Food Science and Technology, Shanghai Ocean University, Shanghai, P. R. China.

Dipeptidyl peptidase-IV (DPP-IV) inhibitors can reduce the blood sugar levels of diabetic patients by preventing the rapid decomposition of incretin hormone and prolonging its physiological effects. In this study, DPP-IV inhibitory peptides FAGDDAPR and LAPPRGSL were isolated from defatted Antarctic krill powder (DAKP) protein by the sequential purification of ultrafiltration, gel filtration chromatography, and RP-HPLC, and IC values of the two peptides were 349.70 ± 3. Read More

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Cholesterol-binding ability of saponin from Japanese starfish.

J Food Sci Technol 2021 Aug 25;58(8):3056-3064. Epub 2020 Sep 25.

Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477 Japan.

Steroidal gylcosides are the predominant metabolites of starfish and are responsible for various biological activities. Some of these activities are recognized as a part of self-defense mechanism of starfish. Cholesterol-binding ability was evaluated with seven starfish crude extracts, where significantly ( < 0. Read More

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Effect of pulsed light treatment on enzymes and protein allergens associated with their structural changes: a review.

Authors:
Abeer S Alhendi

J Food Sci Technol 2021 Aug 18;58(8):2853-2862. Epub 2020 Nov 18.

Quality Control Department, Grain Board of Iraq, Ministry of Trade, Al Taji, Baghdad, Iraq.

The pulsed light (PL) technique is used for food and surface decontamination widely. The sterilization effect of PL is well known and identified as the photo-chemical effect. Besides, PL is used to inactivate enzymes, reduce the immunoreactivity of proteins, and change protein function properties at a laboratory level. Read More

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Evaluating Directional Dependency of Selective Laser Sintered Patient Specific Biodegradable Devices to Improve Predictive Modeling and Design Verification.

Ann Biomed Eng 2021 Jul 21. Epub 2021 Jul 21.

Department of Biomedical Engineering and Center for 3D Medical Fabrication (3DMedFab), Georgia Institute of Technology, Atlanta, GA, 30332, USA.

Additive manufacturing, or 3D printing, of the bioresorbable polymer [Formula: see text]-polycaprolactone (PCL) is an emerging tissue engineering solution addressing patient specific anatomies. Predictively modeling the mechanical behavior of 3D printed parts comprised of PCL improves the ability to develop patient specific devices that meet design requirements while reducing the testing of extraneous design variants and development time for emergency devices. Predicting mechanical behavior of 3D-printed devices is limited by the variability of effective material moduli that are determined in part by the 3D printing manufacturing process. Read More

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Purification and Characterization of a New Halocin HA4 from Haloferax larsenii HA4 Isolated from a Salt Lake.

Probiotics Antimicrob Proteins 2021 Jul 20. Epub 2021 Jul 20.

Department of Genetics, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.

Halocins are antimicrobial peptides secreted by different members of haloarchaea. Halocin HA4 was purified from Haloferax larsenii HA4 using combination of ultrafiltration and chromatographic techniques. It was found to be ~ 14 kDa with unique N-terminal sequence, HN-AEEEIFXPDX, which did not show homology with the known sequence suggesting a new/novel compound. Read More

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Effect of organosolv extraction on the structure and antioxidant activity of eucalyptus kraft lignin.

Int J Biol Macromol 2021 Jul 16;187:462-470. Epub 2021 Jul 16.

College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, PR China; Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, Nanning 530004, PR China. Electronic address:

In this study, three lignin fractions F1, F2, and F3 were obtained from eucalyptus kraft lignin (EKL) by solvent extraction with ethyl acetate, acetone, and acetone/water. The antioxidant activity of these lignin fractions were measured and evaluated by using the DPPH radical scavenging activity, ABTS radical cation scavenging activity and ferric reducing antioxidant power analysis. These lignin fractions were also characterized by applying Gel permeation chromatography, Fourier transform infrared, P NMR and 2D HSQC NMR techniques. Read More

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Submerged cultivation, characterization and in vitro antitumor activity of polysaccharides from Schizophyllum radiatum.

Int J Biol Macromol 2021 Jul 16;186:919-932. Epub 2021 Jul 16.

Grupo Biopolimer, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia UdeA, Calle 70 No. 52 - 21, Medellín 050010, Colombia. Electronic address:

Production of polysaccharides by white-rot-fungi in submerged cultivation has several advantages due to process control. This work deals with the submerged cultivation, extraction and antitumor activity of polysaccharides from a wild strain of Schizophyllum radiatum isolated from a tropical forest of Colombia. The mushroom was cultivated in laboratory conditions, and classified by classical and molecular taxonomy. Read More

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Size-exclusion chromatography selective cleanup of aflatoxins in oilseeds followed by HPLC determination to assess the potential health risk.

Toxicon 2021 Jul 17;200:110-117. Epub 2021 Jul 17.

Environment and Bio-Agriculture Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

Aflatoxins (AFs) are one of the most harmful carcinogenic natural toxins that affect food. Crops containing reasonably high oil content may be affected by Aspergillus species and consequently by AF contamination. In this study, a proposed testing method for AF detection in oilseed was developed, validated, and used for a market survey to assess the probabilistic risk exposure caused by consuming contaminated oilseeds including corn, sunflower seed, and soybean. Read More

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Hypocholesterolemic Effect of Blackcurrant () Extract in Healthy Female Subjects: A Pilot Study.

Molecules 2021 Jul 4;26(13). Epub 2021 Jul 4.

Department of Nursing Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki 036-8564, Japan.

Blackcurrant extract (BCE) ameliorates dyslipidemia in menopausal model animals and in elderly women at a risk of dyslipidemia. However, it is unknown whether the daily intake of BCE can prevent lipid abnormalities in healthy individuals. Lipids are essential for the body, but they also cause arteriosclerosis. Read More

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Correlation of Secondary Particle Number with the Debye-Hückel Parameter for Thickening Mesoporous Silica Shells Formed on Spherical Cores.

ACS Omega 2021 Jul 30;6(27):17734-17740. Epub 2021 Jun 30.

Department of Chemical Engineering, Tohoku University, 6-6-07 Aoba, Aramaki-aza, Aoba-ku, Sendai, Miyagi 980-8579, Japan.

Mesoporous silica shells were formed on nonporous spherical silica cores during the sol-gel reaction to elucidate the mechanism for the generation of secondary particles that disturb the efficient growth of mesoporous shells on the cores. Sodium bromide (NaBr) was used as a typical electrolyte for the sol-gel reaction to increase the ionic strength of the reactant solution, which effectively suppressed the generation of secondary particles during the reaction wherein a uniform mesoporous shell was formed on the spherical core. The number of secondary particles ( ) generated at an ethanol/water weight ratio of 0. Read More

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