8,148 results match your criteria gating analysis

Progression of behavioral deficits during periadolescent development differs in female and male DISC1 knockout rats.

Genes Brain Behav 2021 May 7:e12741. Epub 2021 May 7.

Department of Psychology, Colby College, Waterville, Maine, USA.

Mutations in the disrupted in schizophrenia-1 (DISC1) gene are associated with an increased risk of developing psychological disorders including schizophrenia, bipolar disorder, and depression. Assessing the impact of knocking out genes, like DISC1, in animal models provides valuable insights into the relationship between the gene and behavioral outcomes. Previous research has relied on mouse models to assess these impacts, however these may not yield as reliable or rich a behavioral analysis as can be obtained using rats. Read More

View Article and Full-Text PDF

Effect of ECG-gating retinal photographs on retinal vessel caliber measurements in subjects with and without type 2 diabetes.

Curr Eye Res 2021 May 7. Epub 2021 May 7.

Department of Cardiology, Westmead Hospital, Sydney, Australia.

Purpose/Aim of this study:Retinal vessel caliber is an independent risk marker of cardiovascular disease risk. However, variable mechanical delays in capturing retinal photographs and cardiac cycle-induced retinal vascular changes have shown to reduce the accuracy of retinal vessel caliber measurements, but this has only ever been investigated in healthy subjects. This cross-sectional study is the first study to investigate this issue in type 2 diabetes. Read More

View Article and Full-Text PDF

Free-Breathing Abdominal Magnetic Resonance Fingerprinting Using a Pilot Tone Navigator.

J Magn Reson Imaging 2021 May 5. Epub 2021 May 5.

Department of Radiology, Case Western Reserve University, Cleveland, Ohio, USA.

Background: Quantitative T1 and T2 mapping in the abdomen provides valuable information in tissue characterization but is technically challenging due to respiratory motions. The proposed technique integrates magnetic resonance fingerprinting (MRF) and pilot tone (PT) navigator with retrospective gating to provide simultaneous quantification of multiple tissue properties in a single acquisition without breath-holding or patient set-up.

Purpose: To develop a free-breathing abdominal MRF technique for quantitative mapping in the abdomen. Read More

View Article and Full-Text PDF

Determining clinically relevant features in cytometry data using persistent homology.

bioRxiv 2021 Apr 27. Epub 2021 Apr 27.

Cytometry experiments yield high-dimensional point cloud data that is difficult to interpret manually. Boolean gating techniques coupled with comparisons of relative abundances of cellular subsets is the current standard for cytometry data analysis. However, this approach is unable to capture more subtle topological features hidden in data, especially if those features are further masked by data transforms or significant batch effects or donor-to-donor variations in clinical data. Read More

View Article and Full-Text PDF

Electrostatic gating of single-molecule junctions based on the STM-BJ technique.

Nanoscale 2021 Apr;13(16):7600-7605

State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, iChEM, Xiamen University, 361005, Xiamen, China.

The gating of charge transport through single-molecule junctions is considered a critical step towards molecular circuits but remains challenging. In this work, we report an electrostatic gating method to tune the conductance of single-molecule junctions using the scanning tunneling microscope break junction (STM-BJ) technique incorporated with a back-gated chip as a substrate. We demonstrated that the conductance varied at different applied gating voltages (Vgs). Read More

View Article and Full-Text PDF

A simple, open and extensible gating control unit for cardiac and respiratory synchronisation control in small animal MRI and demonstration of its robust performance in steady-state maintained CINE-MRI.

Magn Reson Imaging 2021 Apr 24. Epub 2021 Apr 24.

Department of Oncology, University of Oxford, Old Road Campus Research Building, Off Roosevelt Drive, Oxford OX3 7DQ, United Kingdom. Electronic address:

Prospective cardiac gating during MRI is hampered by electromagnetic induction from the rapidly switched imaging gradients into the ECG detection circuit. This is particularly challenging in small animal MRI, as higher heart rates combined with a smaller myocardial mass render routine ECG detection challenging. We have developed an open-hardware system that enables continuously running MRI scans to be performed in conjunction with cardio-respiratory gating such that the relaxation-weighted steady state magnetisation is maintained throughout the scan. Read More

View Article and Full-Text PDF

A conserved residue in the P2X4 receptor has a non-conserved function in ATP recognition.

J Biol Chem 2021 Apr 23:100655. Epub 2021 Apr 23.

School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 210009, China;. Electronic address:

Highly conserved amino acids are generally anticipated to have similar functions across a protein superfamily, including that of the P2X ion channels, which are gated by extracellular ATP. However, whether and how these functions are conserved becomes less clear when neighboring amino acids are not conserved. Here, we investigate one such case, focused on the highly conserved residue from P2X4, E118 (rat P2X4 numbering, rP2X4), a P2X subtype associated with human neuropathic pain. Read More

View Article and Full-Text PDF

Analysis of Hemichannels and Gap Junctions: Application and Extension of the Passive Transmembrane Ion Transport Model.

Front Cell Neurosci 2021 7;15:596953. Epub 2021 Apr 7.

School of Mathematics, South China University of Technology, Guangzhou, China.

Electrical synaptic transmission is an essential form of interneuronal communication which is mediated by gap junctions that permit ion flow. Three gene families (connexins, innexins, and pannexins) have evolved to form gap junctional channels. Each gap junctional channel is formed by the docking of the hemichannel of one cell with the corresponding hemichannel of an adjacent cell. Read More

View Article and Full-Text PDF

Slow recovery from the inactivation of voltage-gated sodium channel Nav1.3 in mouse taste receptor cells.

Yoshitaka Ohtubo

Pflugers Arch 2021 Apr 21. Epub 2021 Apr 21.

Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Hibikino 2-4, Kitakyushu, 808-0196, Japan.

Action potentials play an important role in neurotransmitter release in response to taste. Here, I have investigated voltage-gated Na channels, a primary component of action potentials, in respective cell types of mouse fungiform taste bud cells (TBCs) with in situ whole-cell clamping and single-cell RT-PCR techniques. The cell types of TBCs electrophysiologically examined were determined immunohistochemically using the type III inositol 1,4,5-triphoshate receptor as a type II cell marker and synaptosomal-associated protein 25 as a type III cell marker. Read More

View Article and Full-Text PDF

Enhancement of in vivo cardiac photoacoustic signal specificity using spatiotemporal singular value decomposition.

J Biomed Opt 2021 Apr;26(4)

University of Wisconsin-Madison, Department of ECE, Madison, Wisconsin, United States.

Significance: Photoacoustic imaging (PAI) can be used to infer molecular information about myocardial health non-invasively in vivo using optical excitation at ultrasonic spatial resolution. For clinical and preclinical linear array imaging systems, conventional delay-and-sum (DAS) beamforming is typically used. However, DAS cardiac PA images are prone to artifacts such as diffuse quasi-static clutter with temporally varying noise-reducing myocardial signal specificity. Read More

View Article and Full-Text PDF

Frequency of monocyte subsets is linked to the severity of atherosclerosis in patients with ischemic heart disease: A case-control study.

Biomedicine (Taipei) 2020 5;10(2):36-47. Epub 2020 Jun 5.

Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia.

Background: Monocytes are recognized as central cells in the progression of atherosclerosis, and are subcategorized into classical (CD14++CD16), intermediate (CD14++CD16) and non-classical (CD14+CD16) subsets.

Purpose: The present study aimed to assess the relationships between different subsets of monocytes, metabolic and inflammatory factors in patients with stable coronary heart disease.

Methods: A total of 26 patients (both men and women) with stable ischemic heart disease (IHD) were recruited. Read More

View Article and Full-Text PDF

An overview of continuous and discrete phasor analysis of binned or time-gated periodic decays.

X Michalet

Proc SPIE Int Soc Opt Eng 2021 Mar 5;11648. Epub 2021 Mar 5.

Department of Chemistry & Biochemistry, 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA.

Time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of IRF offset, decay truncation and gate shape are also discussed. Read More

View Article and Full-Text PDF

Does pericentral mu-rhythm "power" corticomotor excitability? - A matter of EEG perspective.

Brain Stimul 2021 Apr 20;14(3):713-722. Epub 2021 Apr 20.

Danish Research Centre for Magnetic Resonance, Centre for Functional and Diagnostic Imaging and Research, Copenhagen University Hospital, Amager and Hvidovre, Hvidovre, Denmark; Department of Neurology, Copenhagen University Hospital Bispebjerg, Copenhagen, Denmark; Institute for Clinical Medicine, Faculty of Medical and Health Sciences, University of Copenhagen, Copenhagen, Denmark.

Background: Electroencephalography (EEG) and single-pulse transcranial magnetic stimulation (spTMS) of the primary motor hand area (M1-HAND) have been combined to explore whether the instantaneous expression of pericentral mu-rhythm drives fluctuations in corticomotor excitability, but this line of research has yielded diverging results.

Objectives: To re-assess the relationship between the mu-rhythm power expressed in left pericentral cortex and the amplitude of motor potentials (MEP) evoked with spTMS in left M1-HAND.

Methods: 15 non-preselected healthy young participants received spTMS to the motor hot spot of left M1-HAND. Read More

View Article and Full-Text PDF

Neuropsychological effect of working memory capacity on mental rotation under hypoxia environment.

Int J Psychophysiol 2021 Apr 8;165:18-28. Epub 2021 Apr 8.

Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, School of Psychology, Center for Studies of Psychological Application, Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, China; Plateau Brain Science Research Center, South China Normal University/Tibet University, China. Electronic address:

High-altitude exposure induces the decline of spatial manipulation such as mental rotation which is limited by working memory capacity, but the underlying neuropsychological effect remains to be identified. We evaluated the mental rotation task and the contralateral delay activity (CDA) task under hypoxia environment using the event-related potential. When compared with the controls, the behavior response was slowed on two tasks in the high-altitude group. Read More

View Article and Full-Text PDF

Sound therapy can modulate the functional connectivity of the auditory network.

Prog Neuropsychopharmacol Biol Psychiatry 2021 Apr 7;110:110323. Epub 2021 Apr 7.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China. Electronic address:

The functional connectivity of the auditory network is considered to be important in the development of tinnitus. We hypothesized that sound therapy, as a commonly used effective treatment for tinnitus, can modulate the functional connectivity of the auditory network. In this prospective observational study, we recruited 27 tinnitus patients who had undergone 12 weeks of sound therapy and 27 matched healthy controls. Read More

View Article and Full-Text PDF

Mass-tag barcoding for multiplexed analysis of human synaptosomes and other anuclear events.

Cytometry A 2021 Apr 5. Epub 2021 Apr 5.

Department of Pathology, Stanford University, Stanford, California, USA.

Mass-tag cell barcoding has increased the throughput, multiplexing, and robustness of multiple cytometry approaches. Previously, we adapted mass cytometry for cells to analyze synaptosome preparations (mass synaptometry or SynTOF), extending mass cytometry to these smaller, anuclear particles. To improve throughput and individual event resolution, we report here the application of palladium-based barcoding in human synaptosomes. Read More

View Article and Full-Text PDF

High-dimensional analysis defines multicytokine T-cell subsets and supports a role for IL-21 in atopic dermatitis.

Allergy 2021 Apr 5. Epub 2021 Apr 5.

Department of Dermatology and the Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Background: Flow cytometry is a well-accepted approach for immune profiling; however, its value is restricted by the limited number of markers that can be analyzed simultaneously. Mass cytometry/CyTOF offers broad-scale immune characterization integrating large number of parameters. While partial blood phenotyping was reported in atopic dermatitis (AD), patients' comprehensive profiling, critical for leveraging new targeted treatments, is not available. Read More

View Article and Full-Text PDF

Transcriptome and IgH Repertoire Analyses Show That CD11c B Cells Are a Distinct Population With Similarity to B Cells Arising in Autoimmunity and Infection.

Front Immunol 2021 19;12:649458. Epub 2021 Mar 19.

Laboratory of Molecular Biology and Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD, United States.

A distinct B cell population marked by elevated CD11c expression is found in patients with systemic lupus erythematosus (SLE). Cells with a similar phenotype have been described during chronic infection, but variable gating strategies and nomenclature have led to uncertainty of their relationship to each other. We isolated CD11c cells from peripheral blood and characterized them using transcriptome and IgH repertoire analyses. Read More

View Article and Full-Text PDF

CF Fungal Disease in the Age of CFTR Modulators.

Mycopathologia 2021 Apr 4. Epub 2021 Apr 4.

Respiratory Medicine, Royal Brompton and Harefield NHS Foundation Trust, London, UK.

Fungi are increasingly recognised to have a significant role in the progression of lung disease in Cystic fibrosis with Aspergillus fumigatus the most common fungus isolated during respiratory sampling. The emergence of novel CFTR modulators has, however, significantly changed the outlook of disease progression in CF. In this review we discuss what impact novel CFTR modulators will have on fungal lung disease and its management in CF. Read More

View Article and Full-Text PDF

Assessment of Protocol Impact on Subjectivity Uncertainty When Analyzing Peripheral Blood Mononuclear Cell Flow Cytometry Data Files.

Methods Protoc 2021 Mar 30;4(2). Epub 2021 Mar 30.

Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Loughborough, Leicestershire LE11 3TU, UK.

Measured variability of product within Cell and Gene Therapy (CGT) manufacturing arises from numerous sources across pre-analytical to post-analytical phases of testing. Operators are a function of the manufacturing process and are an important source of variability as a result of personal differences impacted by numerous factors. This research uses measurement uncertainty in comparison to Coefficient of Variation to quantify variation of participants when they complete Flow Cytometry data analysis through a 5-step gating sequence. Read More

View Article and Full-Text PDF

Spinal Inhibitory Interneurons: Gatekeepers of Sensorimotor Pathways.

Int J Mol Sci 2021 Mar 6;22(5). Epub 2021 Mar 6.

Department of Neurobiology and Anatomy, Marion Murray Spinal Cord Research Center, Drexel University College of Medicine, Philadelphia, PA 19129, USA.

The ability to sense and move within an environment are complex functions necessary for the survival of nearly all species. The spinal cord is both the initial entry site for peripheral information and the final output site for motor response, placing spinal circuits as paramount in mediating sensory responses and coordinating movement. This is partly accomplished through the activation of complex spinal microcircuits that gate afferent signals to filter extraneous stimuli from various sensory modalities and determine which signals are transmitted to higher order structures in the CNS and to spinal motor pathways. Read More

View Article and Full-Text PDF

Stereotactic MR-guided online adaptive radiation therapy (SMART) for the treatment of liver metastases in oligometastatic patients: initial clinical experience.

Radiat Oncol J 2021 Mar 26;39(1):33-40. Epub 2021 Mar 26.

Department of Radiation Oncology, Acibadem Mehmet Ali Aydinlar University School of Medicine, Istanbul, Turkey.

Purpose: We aimed to present our initial clinical experience on the implementation of a stereotactic MR-guided online adaptive radiation therapy (SMART) for the treatment of liver metastases in oligometastatic disease.

Materials And Methods: Twenty-one patients (24 lesions) with liver metastasis treated with SMART were included in this retrospective study. Step-and-shoot intensity-modulated radiotherapy technique was used with daily plan adaptation. Read More

View Article and Full-Text PDF

Prospective data-driven respiratory gating of [Ga]Ga-DOTATOC PET/CT.

EJNMMI Res 2021 Mar 31;11(1):33. Epub 2021 Mar 31.

Medical Physics, Uppsala University Hospital, Uppsala, Sweden.

Aim: The aim of this prospective study was to evaluate a data-driven gating software's performance, in terms of identifying the respiratory signal, comparing [Ga]Ga-DOTATOC and [F]FDG examinations. In addition, for the [Ga]Ga-DOTATOC examinations, tracer uptake quantitation and liver lesion detectability were assessed.

Methods: Twenty-four patients with confirmed or suspected neuroendocrine tumours underwent whole-body [Ga]Ga-DOTATOC PET/CT examinations. Read More

View Article and Full-Text PDF

Continuous and discrete phasor analysis of binned or time-gated periodic decays.

Xavier Michalet

AIP Adv 2021 Mar 23;11(3):035331. Epub 2021 Mar 23.

Department of Chemistry and Biochemistry, 607 Charles E. Young Drive E., Los Angeles, California 90095, USA.

The time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived, and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of instrument response function offset, decay truncation, and gate shape are also discussed. Read More

View Article and Full-Text PDF

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time.

J Vis Exp 2021 03 11(169). Epub 2021 Mar 11.

Department of Physiology, Anatomy and Genetics, University of Oxford; Department of Chemistry and Neuroscience Program, Trinity College;

We have developed a method to measure binding of adenine nucleotides to intact, functional transmembrane receptors in a cellular or membrane environment. This method combines expression of proteins tagged with the fluorescent non-canonical amino acid ANAP, and FRET between ANAP and fluorescent (trinitrophenyl) nucleotide derivatives. We present examples of nucleotide binding to ANAP-tagged KATP ion channels measured in unroofed plasma membranes and excised, inside-out membrane patches under voltage clamp. Read More

View Article and Full-Text PDF

Elexacaftor co-potentiates the activity of F508del and gating mutants of CFTR.

J Cyst Fibros 2021 Mar 25. Epub 2021 Mar 25.

Department of Physiology, McGill University, Montréal, Canada; Department of Biochemistry, McGill University, Montréal, Canada.. Electronic address:

Trikafta, the combination of elexacaftor (VX-445), tezacaftor (VX-661) and ivacaftor (VX-770), was approved for therapy of cystic fibrosis (CF) patients with at least one allele of the CFTR mutation F508del. While the corrector function of VX-445 is well established, here we investigated the putative potentiator activity of VX-445 alone and in combination with VX-770. Acute addition of VX-445 increased the VX-770-potentiated F508del- and G551D-CFTR current by ~24% and >70%, respectively, in human bronchial and nasal epithelia. Read More

View Article and Full-Text PDF

Circulating fibrocytes are not disease-specific prognosticators in idiopathic pulmonary fibrosis.

Eur Respir J 2021 Mar 25. Epub 2021 Mar 25.

Division of Respiratory Medicine, NIHR Biomedical Research Centre, University of Nottingham, Nottingham, UK.

Objective: Circulating fibrocytes are elevated in idiopathic pulmonary fibrosis, but the relationship between fibrocyte level with lung function decline and outcomes is lacking replication in prospective clinical study. We aim to validate the utility of circulating fibrocyte levels as a prognostic biomarker in idiopathic pulmonary fibrosis.

Methods: We tested associations between circulating fibrocyte levels, mortality, disease progression and longitudinal lung function in a well-defined prospective observational study of pulmonary fibrosis (PROFILE; NCT01134822). Read More

View Article and Full-Text PDF

K-means quantization for a web-based open-source flow cytometry analysis platform.

Sci Rep 2021 Mar 24;11(1):6735. Epub 2021 Mar 24.

Department of Bioengineering and QB3, UC Berkeley, Berkeley, CA, 94720, USA.

Flow cytometry (FCM) is an analytic technique that is capable of detecting and recording the emission of fluorescence and light scattering of cells or particles (that are collectively called "events") in a population. A typical FCM experiment can produce a large array of data making the analysis computationally intensive. Current FCM data analysis platforms (FlowJo, etc. Read More

View Article and Full-Text PDF

Auditory Sensory Gating of Speech and Nonspeech Stimuli.

J Speech Lang Hear Res 2021 04 23;64(4):1404-1412. Epub 2021 Mar 23.

Department of Audiology and Speech-Language Pathology, University of North Texas, Denton.

Purpose Auditory sensory gating is a neural measure of inhibition and is typically measured with a click or tonal stimulus. This electrophysiological study examined if stimulus characteristics and the use of speech stimuli affected auditory sensory gating indices. Method Auditory event-related potentials were elicited using natural speech, synthetic speech, and nonspeech stimuli in a traditional auditory gating paradigm in 15 adult listeners with normal hearing. Read More

View Article and Full-Text PDF

320 Slice CT in Imaging of Congenital Heart Diseases in Infants: A Single-Center Experience.

Cureus 2021 Feb 15;13(2):e13348. Epub 2021 Feb 15.

Pediatric Radiology, Akron Children's Hospital, Akron, USA.

Objective The study was conducted to evaluate the best possible imaging technique for neonatal cardiac imaging including optimal injection techniques, intravenous line placement, expected radiation dose, and need for sedation while performing the study on a 320 slice Toshiba® Aquilion ONE® scanner. Study results can be used to optimize imaging parameters for maximum clinical yield. We provide representative images of our cases. Read More

View Article and Full-Text PDF
February 2021