499,543 results match your criteria fungi bacterial

Research Note: Characterization of antibiotic resistant phenotypes and linked genes of Escherichia coli and Klebsiella pneumoniae from healthy broiler chickens, Karnataka, India.

Poult Sci 2021 Mar 22;100(6):101094. Epub 2021 Mar 22.

Animal Disease Diagnostic Laboratory, Government Veterinary Hospital, Jail Road, Karangalpady, Mangalore, 575002, Karnataka, India.

The study was carried out to estimate the burden and pattern of antibiotic resistance and to identify antibiotic resistance genes with focus on ESBL producers, plasmid mediated quinolone resistance, and tetracycline efflux genes, in faecal bacterial isolates collected from poultry farms of coastal Southern Karnataka, India. High resistance to fluoroquinolones was observed with 94% Escherichia coli and 80% Klebsiella pneumoniae being resistant to both ciprofloxacin and levofloxacin. All the Escherichia coli strains were resistant to tetracycline (100%). Read More

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Nanoscale dynamics of peptidoglycan assembly during the cell cycle of Streptococcus pneumoniae.

Curr Biol 2021 May 7. Epub 2021 May 7.

Univ. Grenoble Alpes, CNRS, CEA, IBS, 38000 Grenoble, France. Electronic address:

Dynamics of cell elongation and septation are key determinants of bacterial morphogenesis. These processes are intimately linked to peptidoglycan synthesis performed by macromolecular complexes called the elongasome and the divisome. In rod-shaped bacteria, cell elongation and septation, which are dissociated in time and space, have been well described. Read More

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Helicobacter pylori CagA elicits BRCAness to induce genome instability that may underlie bacterial gastric carcinogenesis.

Cell Host Microbe 2021 May 6. Epub 2021 May 6.

Department of Microbiology, Graduate School of Medicine, the University of Tokyo, Tokyo 113-0033, Japan. Electronic address:

Infection with CagA-producing Helicobacter pylori plays a causative role in the development of gastric cancer. Upon delivery into gastric epithelial cells, CagA deregulates prooncogenic phosphatase SHP2 while inhibiting polarity-regulating kinase PAR1b through complex formation. Here, we show that CagA/PAR1b interaction subverts nuclear translocation of BRCA1 by inhibiting PAR1b-mediated BRCA1 phosphorylation. Read More

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Zinc limitation triggers anticipatory adaptations in Mycobacterium tuberculosis.

PLoS Pathog 2021 May 14;17(5):e1009570. Epub 2021 May 14.

School of Life Sciences, University of Hawai'i at Mānoa, Honolulu, Hawaii, United States of America.

Mycobacterium tuberculosis (Mtb) has complex and dynamic interactions with the human host, and subpopulations of Mtb that emerge during infection can influence disease outcomes. This study implicates zinc ion (Zn2+) availability as a likely driver of bacterial phenotypic heterogeneity in vivo. Zn2+ sequestration is part of "nutritional immunity", where the immune system limits micronutrients to control pathogen growth, but this defense mechanism seems to be ineffective in controlling Mtb infection. Read More

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Characterization of Bacillus nealsonii strain KBH10 capable of reducing aqueous mercury in laboratory-scale reactor.

Water Sci Technol 2021 May;83(9):2287-2295

Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan E-mail:

The environmental release of mercury is continuously increasing with high degree of mobility, transformation and amplified toxicity. Improving remediation strategies is becoming increasingly important to achieve more stringent environmental safety standards. This study develops a laboratory-scale reactor for bioremediation of aqueous mercury using a biofilm-producing bacterial strain, KBH10, isolated from mercury-polluted soil. Read More

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Relationships Between Bronchoscopy, Microbiology, and Radiology in Noncystic Fibrosis Bronchiectasis.

Pediatr Allergy Immunol Pulmonol 2021 May 14. Epub 2021 May 14.

Department of Pediatric Pulmonolgy, Bezmialem Vakif University Medical Faculty, Istanbul, Turkey.

Published data on the correlations of bronchoscopy findings with microbiological, radiological, and pulmonary function test results in children with noncystic fibrosis (CF) bronchiectasis (BE) are unavailable. The aims of this study were to evaluate relationships between Bronchoscopic appearance and secretion scoring, microbiological growth, radiological severity level, and pulmonary function tests in patients with non-CF BE. Children with non-CF BE were identified and collected over a 6-year period. Read More

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Biosynthesis of silver nano-drug using and its synergistic antibacterial activity against multidrug-resistant bacteria for wound dressing applications.

3 Biotech 2021 Jun 8;11(6):255. Epub 2021 May 8.

Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif, 21944 Saudi Arabia.

We report here the synthesis of silver nanoparticles (AgNPs) from an aqueous extract of and their use as an antimicrobial agent on their own or in combination with antibiotics in inhibiting multidrug-resistant bacteria (MDR). One strategy of bacterial infection control in wound healing is AgNP biosynthesis. We collected bacterial strains of patient skin infections from Al-Adwani Hospital. Read More

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Colistin Resistance among Isolated from Clinical Samples in Gaza Strip.

Can J Infect Dis Med Microbiol 2021 20;2021:6634684. Epub 2021 Apr 20.

Department of Medical Laboratory Sciences Faculty of Health Sciences Islamic University of Gaza, Gaza Strip, Gaza, Palestine, State of Palestine.

Bacterial infections, especially drug-resistant infections, are a major global health issue. The emergence of multidrug-resistant (MDR) strains of and the lack of new antibiotics have worrisome prospects for all of humanity. Colistin is considered the last-line drug for MDR Gram-negative bacteria (GNB), and it is often used for treatment of respiratory infections caused by MDR-GNB. Read More

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Assessment of Airborne Bacterial and Fungal Communities in Shahrekord Hospitals.

J Environ Public Health 2021 24;2021:8864051. Epub 2021 Apr 24.

Department of Environmental Health Engineering, School of Health, Tehran University of Medical Sciences, Tehran, Iran.

This paper presents information about airborne microorganisms (bacteria and fungi) in the indoor air of two hospitals (Kashani and Hajar) in the city of Shahrekord, Iran. The settle plate technique using open Petri dishes containing different culture media was employed to collect a sample and using Quick Take 30 Sample Pump three days per week for a period of 8 weeks. Standard microbiological methods were employed for the identification of bacterial and fungal isolates. Read More

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The Molecular Information About Deadwood Bacteriomes Partly Depends on the Targeted Environmental DNA.

Front Microbiol 2021 27;12:640386. Epub 2021 Apr 27.

Department of Microbiology, University of Innsbruck, Innsbruck, Austria.

Microbiome studies mostly rely on total DNA extracts obtained directly from environmental samples. The total DNA consists of both intra- and extracellular DNA, which differ in terms of their ecological interpretation. In the present study, we have investigated for the first time the differences among the three DNA types using microbiome sequencing of deadwood logs (Hunter decay classes I, III, and V). Read More

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Shifts in the Oral Microbiota During a Four-Week Commercial Saturation Dive to 200 Meters.

Front Physiol 2021 27;12:669355. Epub 2021 Apr 27.

Department of Circulation and Medical Imaging, Faculty of Medicine and Health Sciences, NTNU Norwegian University of Science and Technology, Trondheim, Norway.

During commercial saturation diving, divers live and work under hyperbaric and hyperoxic conditions. The myriads of bacteria that live in and on the human body must adjust to the resultant hyperbaric stress. In this study, we examined the shifts in bacterial content in the oral cavity of saturation divers, using a metagenomic approach to determine the diversity in the composition of bacterial phyla and genera in saliva from 23 male divers before, during, and immediately after 4 weeks of commercial heliox saturation diving to a working depth of circa 200 m. Read More

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MC-100093, a novel β-lactam GLT-1 enhancer devoid of antimicrobial properties attenuates cocaine relapse in rats.

J Pharmacol Exp Ther 2021 May 13. Epub 2021 May 13.

School of Pharmacy, Temple University, United States.

Cocaine use disorder (CUD) currently lacks FDA-approved treatments. In rodents, the glutamate transporter-1 (GLT-1) is downregulated in the nucleus accumbens following cocaine self-administration and increasing the expression and function of GLT-1 reduces the reinstatement of cocaine-seeking. The beta-lactam antibiotic ceftriaxone upregulates GLT-1 and attenuates cue- and cocaine-induced cocaine seeking without affecting motivation for natural rewards. Read More

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miR-155 and miR-146a collectively regulate meningitic Escherichia coli infection-mediated neuroinflammatory responses.

J Neuroinflammation 2021 May 13;18(1):114. Epub 2021 May 13.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.

Background: Escherichia coli is the most common Gram-negative bacterium causing meningitis, and E. coli meningitis is associated with high mortality and morbidity throughout the world. Our previous study showed that E. Read More

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Phenotypic changes in group B streptococci grown in the presence of the polyols, erythritol, sorbitol and mannitol.

BMC Microbiol 2021 May 13;21(1):145. Epub 2021 May 13.

Division of Diagnostic and Applied Microbiology, Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada.

Background: Group B streptococci (GBS) are important neonatal bacterial pathogens that can cause severe invasive disease in the newborn. It is thought that in many cases of invasive neonatal GBS disease, the bacteria ascend the vagina into the uterus and infect the amniotic fluid surrounding the fetus. Important constituents of this environment include the polyols or sugar alcohols of which erythritol, sorbitol and mannitol are examples. Read More

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Both viable and inactivated amoeba spores protect their intracellular bacteria from drinking water disinfection.

J Hazard Mater 2021 May 11;417:126006. Epub 2021 May 11.

School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510006, China; Environmental Microbiomics Research Center, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Guangzhou 510006, China. Electronic address:

In drinking water systems, waterborne pathogens constitute a significant threat. While most studies focus on a single infectious agent, such as bacteria, fungi, viruses, and protists, the effect of interactions among these infectious agents on disinfection treatment has largely been ignored. In this study, we find that dormant amoeba spores, a frequently found protist in drinking water systems, can protect their intracellular bacteria from drinking water disinfection. Read More

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Real-life experience with ceftolozane/tazobactam in Canada; results from the CLEAR (Canadian LEadership on Antimicrobial Real-life usage) registry.

J Glob Antimicrob Resist 2021 May 10. Epub 2021 May 10.

Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada. Electronic address:

Objectives: Ceftolozane/tazobactam is a cephalosporin/β-lactamase inhibitor combination with activity against Gram-negative bacilli. We report the use of ceftolozane/tazobactam in Canada using a national registry.

Methods: The CLEAR registry uses REDCap (Research Electronic Data Capture) (online survey, https://is. Read More

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The PAR promoter expression system: modified lac promoters for controlled recombinant protein production in Escherichia coli.

N Biotechnol 2021 May 10. Epub 2021 May 10.

Institute of Microbiology and Infection and School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK; College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address:

Many commonly used bacterial promoters employed for recombinant protein production (RPP) in Escherichia coli are capable of high-level protein expression. However, such promoter systems are often too strong, being ill suited for expressing proteins that are difficult to fold, targeted to the membrane or secreted out of the cytoplasm. To circumvent this problem, a suite of bacterial promoters has been constructed with a range of different promoter strengths, assigning them specific "promoter activity ratings" (PARs). Read More

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Coevolution between bacterial CRISPR-Cas systems and their bacteriophages.

Cell Host Microbe 2021 May;29(5):715-725

ESI, Biosciences, University of Exeter, Cornwall Campus, Penryn TR10 9FE, UK. Electronic address:

CRISPR-Cas systems provide bacteria and archaea with adaptive, heritable immunity against their viruses (bacteriophages and phages) and other parasitic genetic elements. CRISPR-Cas systems are highly diverse, and we are only beginning to understand their relative importance in phage defense. In this review, we will discuss when and why CRISPR-Cas immunity against phages evolves, and how this, in turn, selects for the evolution of immune evasion by phages. Read More

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Rickettsial infections: A blind spot in our view of neglected tropical diseases.

PLoS Negl Trop Dis 2021 May 13;15(5):e0009353. Epub 2021 May 13.

Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.

Rickettsial diseases are a group of vector-borne bacterial infections that cause acute febrile illness with potentially severe or fatal complications. These vector-borne diseases are prevalent in tropical and subtropical regions worldwide and disproportionately affect poorer communities but are scientifically underrecognized. Despite this, they are not included in the World Health Organization's list of neglected tropical diseases nor were they mentioned in Peter Hotez's recent reflections on "What constitutes a neglected tropical disease?" in PLOS Neglected Tropical Diseases [1]. Read More

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Understanding the differences in 2G ethanol fermentative scales through omics data integration.

FEMS Yeast Res 2021 May 13. Epub 2021 May 13.

Department of Genetics, Evolution, Microbiology, and Immunology, Institute of Biology, University of Campinas, Campinas, SP, 13083-862, Brazil.

In this work, we evaluated the fermentative performance and metabolism modifications of a second generation (2G) industrial yeast by comparing an industrial condition during laboratory and industrial scale fermentations. Fermentations were done using industrial lignocellulosic hydrolysate and a synthetic medium containing inhibitors and analyses were carried out through transcriptomics and proteomics of these experimental conditions. We found that fermentation profiles were very similar, but there was an increase in xylose consumption rate during fermentations using synthetic medium when compared to lignocellulosic hydrolysate, likely due to the presence of unknown growth inhibitors contained in the hydrolysate. Read More

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[Research progress on natural products of fungi of Diaporthe sp.]

Zhongguo Zhong Yao Za Zhi 2021 Apr;46(7):1717-1726

Hunan University of Traditional Chinese Medicine Changsha 410208, China Hunan Engineering Technology Center of Standardization and Function of Chinese Herbal Decoction Pieces Changsha 410208, China.

Diaporthe sp. fungi is one of the important sources of active natural products. Polyketides, alkaloids, terpenes, anthraquinones and other types of novel metabolic products are found from this genus, and many of them have significant anti-tumor, antibacterial, anti-hyperlipidemia, inhibition of pulmonary fibrosis, antioxidant and other biological activities. Read More

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Targeting the Gut Microbiota for Remediating Obesity and Related Metabolic Disorders.

J Nutr 2021 May 12. Epub 2021 May 12.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.

The rate of obesity is rapidly increasing and has become a health and economic burden worldwide. As recent studies have revealed that the gut microbiota is closely linked to obesity, researchers have used various approaches to modulate the gut microbiota to treat the condition. Dietary composition and energy intake strongly affect the composition and function of the gut microbiota. Read More

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Metagenome dataset of lateritic soil microbiota from Sadaipur, Birbhum, West Bengal, India.

Data Brief 2021 Jun 6;36:107041. Epub 2021 Apr 6.

Post Graduate Department of Biotechnology, St. Xavier's College (Autonomous), 30 Mother Teresa Sarani (Park Street), Kolkata, West Bengal 700016, India.

The data represents the bacterial community profile obtained through metagenomic sequencing of soil sample, collected from the 'Rarh' region of West Bengal, which is characterized by the lateritic badlands dating back to the late Pleistocene. Taxonomic binning and operational taxonomic unit (OTU) prediction of the Illumina sequencing data indicated the abundance Proteobacteria (61%) followed closely by Bacterioidetes (35%). The top two most abundant genera identified, were Sphingobacterium and Acinetobacter respectively. Read More

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Bactericidal/Permeability-Increasing Protein Preeminently Mediates Clearance of CD18-Dependent Phagocytosis.

Front Immunol 2021 26;12:659523. Epub 2021 Apr 26.

Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, United States.

Chronic infection mysteriously occurs in the airways of patients with cystic fibrosis (CF), bronchiectasis (BE), and chronic obstructive pulmonary disease (COPD) in the absence of neutrophil dysfunction or neutropenia and is strongly associated with autoimmunity to bactericidal permeability-increasing protein (BPI). Here, we define a critical role for BPI in immunity against Wild type and BPI-deficient ( mice were infected with , and bacterial clearance, cell infiltrates, cytokine production, and phagocytosis were quantified. mice exhibited a decreased ability to clear in concert with increased neutrophil counts and cytokine release. Read More

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Impact of on the Development of High Level Colistin Resistance in and .

Front Microbiol 2021 26;12:666782. Epub 2021 Apr 26.

College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, Key Laboratory of Zoonosis of Ministry of Agricultural and Rural Affairs, National Risk Assessment Laboratory for Antimicrobial Resistance of Microorganisms in Animals, South China Agricultural University, Guangzhou, China.

Plasmid-mediated colistin resistance gene generally confers low-level resistance. The purpose of this study was to investigate the impact of on the development of high-level colistin resistance (HLCR) in and . In this study, -negative and strains and their corresponding -positive transformants were used to generate HLCR mutants multiple passages in the presence of increasing concentrations of colistin. Read More

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The lower respiratory tract microbiome of critically ill patients with COVID-19.

Sci Rep 2021 05 12;11(1):10103. Epub 2021 May 12.

Operative Unit of Clinical Microbiology, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 9 via G. Massarenti, 40138, Bologna, Italy.

COVID-19 infection may predispose to secondary bacterial infection which is associated with poor clinical outcome especially among critically ill patients. We aimed to characterize the lower respiratory tract bacterial microbiome of COVID-19 critically ill patients in comparison to COVID-19-negative patients. We performed a 16S rRNA profiling on bronchoalveolar lavage (BAL) samples collected between April and May 2020 from 24 COVID-19 critically ill subjects and 24 patients with non-COVID-19 pneumonia. Read More

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mSphere of Influence: Surface Sensing in Biofilm Formation.

Kara B De Leόn

mSphere 2021 May 12;6(3). Epub 2021 May 12.

Department of Microbiology and Plant Biology, University of Oklahoma, Norman, Oklahoma, USA

Kara B. De Leόn works in the field of microbial ecology, environmental biofilms, and microbial genetics. In this mSphere of Influence article, she reflects on how the paper "Multigenerational memory and adaptive adhesion in early bacterial biofilm communities" by C. Read More

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Lsr2 and Its Novel Paralogue Mediate the Adjustment of Mycobacterium smegmatis to Unfavorable Environmental Conditions.

mSphere 2021 May 12;6(3). Epub 2021 May 12.

Department of Molecular Microbiology, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

Lsr2 is a nucleoid-associated protein (NAP) that has been found strictly in actinobacteria, including mycobacteria. It is a functional homolog of histone-like nucleoid-structuring protein (H-NS); it acts as a DNA-bridging protein that plays a role in chromosomal organization and transcriptional regulation. To date, the studies on Lsr2 have focused mainly on In this study, we analyze the role of Lsr2 as a transcription factor in , a saprophytic bacterium whose natural habitat (soil and water) substantially differs from those of the obligatory mycobacterial pathogens. Read More

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Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands.

mSphere 2021 May 12;6(3). Epub 2021 May 12.

Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic

is a leading opportunistic pathogen causing nosocomial infections that is notable for its ability to form a biofilm and for its high rates of antibiotic resistance. It serves as a reservoir of multiple antimicrobial resistance genes that spread among the staphylococcal population by horizontal gene transfer such as transduction. While phage-mediated transduction is well studied in , transducing phages have not been described in detail yet. Read More

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Genome Investigation of Urinary Strains and Their Relationship to Isolates of the Vaginal Microbiota.

mSphere 2021 May 12;6(3). Epub 2021 May 12.

Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA.

is a frequent member of the urogenital microbiota. Given the association between and bacterial vaginosis (BV), significant efforts have been focused on characterizing this species in the vaginal microbiota. However, also is a frequent member of the urinary microbiota. Read More

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