Ocul Surf 2021 May 5. Epub 2021 May 5.
State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao, China; Qingdao Eye Hospital of Shandong First Medical University, Qingdao, China; Eye Hospital of Shandong First Medical University, Jinan, China. Electronic address:
Purpose: The corneal limbus maintains the homeostasis, immune and angiogenic privilege of cornea. This study aimed to depict the landscape of human limbus tissues by single-cell RNA sequencing (scRNA-seq).
Methods: Single cells of human limbus collected from donor corneas were performed scRNA-seq using 10x Genomics platform, followed by clustering cell types through the t-distributed stochastic neighbor embedding (t-SNE) and unbiased computational informatic analysis. Read More