1,405 results match your criteria functionalized biomolecules


A Review of the Antimicrobial Activity of Selenium Nanoparticles.

J Nanosci Nanotechnol 2021 Nov;21(11):5383-5398

Instituto de Investigación en Biociencias, Centro Universitario de Los Altos, Universidad de Guadalajara, Tepatitlán de Morelos, Jalisco, 47600, México.

Antimicrobial resistance has become a severe problem for health systems worldwide, and counteractions are challenging because of the lack of interest of pharmaceutical companies in generating new and effective antimicrobial drugs. Selenium nanoparticles have attracted considerable interest in treating bacteria, fungi, parasites, and viruses of clinical importance due to their high therapeutic efficacy and almost zero generation of adverse effects. Some studies have revealed that the antimicrobial activity of these nanoparticles is due to the generation of reactive oxygen species, but more studies are needed to clarify their antimicrobial mechanisms. Read More

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November 2021

6-Aminopyridine-3-boronic acid functionalized magnetic nanoparticles for highly efficient enrichment of -diol-containing biomolecules.

Anal Methods 2021 May 10. Epub 2021 May 10.

College of Chemistry and Chemical Engineering, Henan Key Laboratory of Function-Oriented Porous Materials, Luoyang Normal University, Luoyang 471022, P. R. China. and Department of Chemistry Bengbu Medical College, 233030, China.

Boronate affinity materials, as efficient sorbents for extraction, separation and enrichment of cis-diol-containing biomolecules, have attracted more and more attention in recent years. However, conventional boronate affinity materials require a basic binding pH (usually 8.5), which gives rise to not only inconvenience in operation but also the risk of degradation of labile compounds, and suffer from low binding affinity, which make the extraction of cis-diol-containing compounds of low concentration difficult or impossible. Read More

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Introducing Bacteria and Synthetic Biomolecules along Engineered DNA Fibers.

Authors:
Jonathan R Burns

Small 2021 May 7:e2100136. Epub 2021 May 7.

Department of Chemistry, Institute of Structural and Molecular Biology, University College London, London, WC1H 0AJ, UK.

Deoxyribonucleic acid (DNA) nanotechnology enables user-defined structures to be built with unrivalled control. The approach is currently restricted across the nanoscale, yet the ability to generate macroscopic DNA structures has enormous potential with applications spanning material, physical, and biological science. To address this need, I employed DNA nanotechnology and developed a new macromolecular nanoarchitectonic assembly method to produce DNA fibers with customizable properties. Read More

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Microbial Fabricated Nanosystems: Applications in Drug Delivery and Targeting.

Front Chem 2021 20;9:617353. Epub 2021 Apr 20.

Department of Biochemistry and Biotechnology, Sardar Bhagwan Singh University, Dehradun, India.

The emergence of nanosystems for different biomedical and drug delivery applications has drawn the attention of researchers worldwide. The likeness of microorganisms including bacteria, yeast, algae, fungi, and even viruses toward metals is well-known. Higher tolerance to toxic metals has opened up new avenues of designing microbial fabricated nanomaterials. Read More

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Antifouling Peptide Hydrogel Based Electrochemical Biosensors for Highly Sensitive Detection of Cancer Biomarker HER2 in Human Serum.

Anal Chem 2021 May 6. Epub 2021 May 6.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.

A facile strategy for the electrochemical detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker, was presented via the fabrication of an antifouling sensing interface based on the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and a biocompatible peptide hydrogel. The peptide hydrogel prepared from a designed short peptide of Phe-Glu-Lys-Phe functionalized with a fluorene methoxycarbonyl group (Fmoc-FEKF) enabled excellent activity preservation for the immobilized biomolecules, and its good hydrophilicity facilitated effective alleviation of nonspecific adsorption or biofouling, while the PEDOT film provided a highly stable and conducting substrate. The developed biosensor was highly sensitive and selective for HER2 detection, with a wide linear response range from 0. Read More

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The unpredictable carbon nanotube biocorona and a functionalization method to prevent protein biofouling.

J Nanobiotechnology 2021 May 5;19(1):129. Epub 2021 May 5.

The Nanomedicine Group, University of Cantabria-IDIVAL, 39011, Santander, Spain.

Background: The intrinsic physicochemical properties of carbon nanotubes (CNTs) make them unique tools in nanotechnology. Their elemental composition, resilience, thermal properties, and surface reactivity make CNTs also of undisputed interest in biotechnology. In particular, their extraordinary ability to capture biomolecules on their surface makes them essential in this field. Read More

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Chitosan Functionalized Magnetic Nanoparticles to Provide Neural Regeneration and Recovery after Experimental Model Induced Peripheral Nerve Injury.

Biomolecules 2021 Apr 30;11(5). Epub 2021 Apr 30.

Department of Physiology, Iuliu Hațieganu University of Medicine and Pharmacy Cluj-Napoca, Clinicilor Street No. 1-3, 400006 Cluj-Napoca, Cluj County, Romania.

(1) Background: Peripheral nerve injuries have a great impact on a patient's quality of life and a generally poor outcome regarding functional recovery. Lately, studies have focused on different types of nanoparticles and various natural substances for the treatment of peripheral nerve injuries. This is the case of chitosan, a natural compound from the crustaceans' exoskeleton. Read More

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Smart gating porous particles as new carriers for drug delivery.

Adv Drug Deliv Rev 2021 Apr 27. Epub 2021 Apr 27.

Univ Lyon, University Claude Bernard Lyon-1, CNRS, ISA-UMR 5280, 69622 Villeurbanne, France. Electronic address:

The design of smart drug delivery carriers has recently attracted great attention in the biomedical field. Smart carriers can specifically respond to physical and chemical changes in their environment, such as temperature, photoirradiation, ultrasound, magnetic field, pH, redox species, and biomolecules. This review summarizes recent advances in the integration of porous particles and stimuli-responsive gatekeepers for effective drug delivery. Read More

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Enantioselective effect of cysteine functionalized mesoporous silica nanoparticles in U87 MG and GM08680 human cells and bacteria.

J Mater Chem B 2021 Apr;9(16):3544-3553

School of Chemistry, CRANN and AMBER Research Centres, Trinity College Dublin, College Green, Dublin 2, Ireland.

Chirality is a fundamental phenomenon in biological systems, since most of the biomolecules and biological components and species are chiral and therefore recognize and respond differently depending on the enantiomer present. With increasing research into the use of nanomaterials for biomedical purposes, it is essential to understand the role that chirality of nanoparticles plays at the cellular level. Here, the chiral cysteine functionalization of mesoporous silica nanoparticles has been shown to broadly affect its interaction with U87 MG human glioblastoma cell, healthy human fibroblast (GM08680) and methicillin-resistant S. Read More

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Soft matter polysaccharide-based hydrogels as versatile bioengineered platforms for brain tissue repair and regeneration.

Int J Biol Macromol 2021 Apr 21;182:1091-1111. Epub 2021 Apr 21.

Department of Preventive Veterinary Medicine, Veterinary School, Federal University of Minas Gerais - UFMG, Brazil.

Acute or chronic brain injuries promote deaths and the life-long debilitating neurological status where, despite advances in therapeutic strategies, clinical outcome hardly achieves total patient recovery. In recent decades, brain tissue engineering emerged as an encouraging area of research for helping in damaged central nervous system (CNS) recovery. Polysaccharides are abundant naturally occurring biomacromolecules with a great potential enhancement of advanced technologies in brain tissue repair and regeneration (BTRR). Read More

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Bio-nano interactions: binding proteins, polysaccharides, lipids and nucleic acids onto magnetic nanoparticles.

Biomater Res 2021 Apr 21;25(1):12. Epub 2021 Apr 21.

Bioseparation Engineering Group, Department of Mechanical Engineering, Technical University of Munich, 85748, Garching bei München, Germany.

The major interest in nanoparticles as an application platform for biotechnology arises from their high surface-to-volume ratio. Iron oxide nanoparticles (IONPs) are particularly appealing due to their superparamagnetic behavior, which enables bioseparation using external magnetic fields. In order to design advanced biomaterials, improve binding capacities and develop innovative processing solutions, a thorough understanding of the factors governing organic-inorganic binding in solution is critical but has not yet been achieved, given the wide variety of chemical and physical influences. Read More

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AAZTA-squaramide ester competing with DOTA-, DTPA- and CHX-A-DTPA-analogues: Promising tool for Lu-labeling of monoclonal antibodies under mild conditions.

Nucl Med Biol 2021 Mar 26;96-97:80-93. Epub 2021 Mar 26.

Department of Chemistry - TRIGA site, Johannes Gutenberg University, Mainz, Germany. Electronic address:

Background: Combining the advantages of both cyclic and acyclic chelator systems, AAZTA (1,4-bis(carboxymethyl)-6-[bis(carboxymethyl)]amino-6-methylperhydro-1,4-diazepine) is well suited for complexation of various diagnostic and therapeutic radiometals such as gallium-68, scandium-44 and lutetium-177 under mild conditions. Due to its specificity for primary amines and pH dependent binding properties, squaric acid (SA) represents an excellent tool for selective coupling of the appropriate chelator to different target vectors. Therefore, the aim of this study was to evaluate radiolabeling properties of the novel bifunctional AAZTA-SA being coupled to a model antibody (bevacizumab) in comparison to DOTA-SA, DTPA-p-Bn-SA and CHX-A″-DTPA-p-Bn-SA using the therapeutic nuclide lutetium-177. Read More

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Interaction of Folic Acid with Mn Doped CdTe/ZnS Quantum Dots: In Situ Detection of Folic Acid.

J Fluoresc 2021 Apr 5. Epub 2021 Apr 5.

School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur, C.G., 492010, India.

To utilize the nanomaterials as an effective carrier for the drug delivery applications, it is important to study the interaction between nanomaterials and drug or biomolecules. In this study GSH functionalized Mn-doped CdTe/ZnS QDs has been utilized as a model nanomaterial due to its high luminescence property. Folic acid (FA) gradually quenches the FL of GSH functionalized Mn - doped CdTe/ZnS QDs. Read More

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Proof-of-Concept Study of Multifunctional Hybrid Nanoparticle System Combined with NIR Laser Irradiation for the Treatment of Melanoma.

Biomolecules 2021 Mar 30;11(4). Epub 2021 Mar 30.

Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisboa, Portugal.

The global impact of cancer emphasizes the importance of developing innovative, effective and minimally invasive therapies. In the context of superficial cancers, the development of a multifunctional nanoparticle-based system and its in vitro and in vivo safety and efficacy characterization are, herein, proposed as a proof-of-concept. This multifunctional system consists of gold nanoparticles coated with hyaluronic and oleic acids, and functionalized with epidermal growth factor for greater specificity towards cutaneous melanoma cells. Read More

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Vapor Sublimation and Deposition to Fabricate a Porous Methyl Propiolate-Functionalized Poly--xylylene Material for Copper-Free Click Chemistry.

Polymers (Basel) 2021 Mar 4;13(5). Epub 2021 Mar 4.

Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.

Conventional porous materials are mostly synthesized in solution-based methods involving solvents and initiators, and the functionalization of these porous materials usually requires additional and complex steps. In the current study, a methyl propiolate-functionalized porous poly--xylylene material was fabricated based on a unique vapor sublimation and deposition process. The process used a water solution and ice as the template with a customizable shape and dimensions, and the conventional chemical vapor deposition (CVD) polymerization of poly--xylylene on such an ice template formed a three-dimensional, porous poly--xylylene material with interconnected porous structures. Read More

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Enhanced Transport and Permeation of a Polymeric Nanocarrier across the Retina by Mixing with ATP upon Intravitreal Injection.

Pharmaceutics 2021 Mar 29;13(4). Epub 2021 Mar 29.

School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea.

The outer part of the retina pigment epithelium (RPE) in the retina is the main site of neovascularization associated with retinal diseases. However, various obstacles interrupt the delivery of medicines across the RPE, mainly due to the well-developed tight junctions in the RPE. Currently, there is no practical formulation to overcome this issue. Read More

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Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations.

Membranes (Basel) 2021 Mar 6;11(3). Epub 2021 Mar 6.

Golden LEAF Biomanufacturing Training and Education Center (BTEC), North Carolina State University, 850 Oval Dr, Raleigh, NC 27695-7905, USA.

This study presents the preparation and characterization of UV-grafted polybutylene terepthalate (PBT) ion exchange nonwoven membranes for chromatographic purification of biomolecules. The PBT nonwoven was functionalized with sulfonate and secondary amine for cation and anion exchange (CEX and AEX), respectively. The anion exchange membrane showed an equilibrium static binding capacity of 1300 mg BSA/g of membrane, while the cationic membranes achieved a maximum equilibrium binding capacity of over 700 mg hIgG/g of membrane. Read More

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Drug-Free Enzyme-Based Bactericidal Nanomotors against Pathogenic Bacteria.

ACS Appl Mater Interfaces 2021 Apr 26;13(13):14964-14973. Epub 2021 Mar 26.

Smart nano-bio-devices, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Baldiri Reixac 10-12, 08028 Barcelona Spain.

The low efficacy of current conventional treatments for bacterial infections increases mortality rates worldwide. To alleviate this global health problem, we propose drug-free enzyme-based nanomotors for the treatment of bacterial urinary-tract infections. We develop nanomotors consisting of mesoporous silica nanoparticles (MSNPs) that were functionalized with either urease (U-MSNPs), lysozyme (L-MSNPs), or urease and lysozyme (M-MSNPs), and use them against nonpathogenic planktonic . Read More

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Ultrasensitive Characterization of the Prion Protein by Surface-Enhanced Raman Scattering: Selective Enhancement via Electrostatic Tethering of the Intrinsically Disordered Domain with Functionalized Silver Nanoparticles.

J Phys Chem Lett 2021 Apr 24;12(12):3187-3194. Epub 2021 Mar 24.

Surface-enhanced Raman scattering (SERS) circumvents the inherent insensitivity of Raman spectroscopy and offers a powerful tool for the ultrasensitive detection and characterization of biomolecules at low concentrations. Here we show that SERS via electrostatic tethering between surface-modified negatively charged silver nanoparticles and highly positively charged intrinsically disordered N-terminal domain of the prion protein allows highly sensitive and reproducible protein detection and characterization at as low as hundreds of nanomolar protein concentrations. These measurements preferentially illuminate a selective part of the protein due to a sharp dependence of the near-field intensity on the distance between the nanoparticle surface and the protein. Read More

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Dual Antibody-Conjugated Amyloid Nanorods to Promote Selective Cell-Cell Interactions.

ACS Appl Mater Interfaces 2021 Apr 24;13(13):14875-14884. Epub 2021 Mar 24.

Institut de Biotecnologia i de Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra (Barcelona) 08193, Spain.

Grafting biomolecules on nanostructures' surfaces is an increasingly used strategy to target pathogenic cells, with both diagnostic and therapeutic applications. However, nanomaterials monofunctionalized by conjugating a single type of ligand find limited uses in pathologies/therapies that require two or more targets/receptors to be targeted and/or activated with a single molecular entity simultaneously. Therefore, multivalent nanomaterials for dual- or multitargeting are attracting significant interest. Read More

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Functionalized ultra-fine bimetallic PtRu alloy nanoparticle with high peroxidase-mimicking activity for rapid and sensitive colorimetric quantification of C-reactive protein.

Mikrochim Acta 2021 Mar 9;188(4):119. Epub 2021 Mar 9.

Department of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan, 426-791, South Korea.

The in situ synthesis is reported of citric acid-functionalized ultra-fine bimetallic PtRu alloy nanoparticles (CA@PtRu ANPs) through a simple one-pot wet chemical method. The cost-efficient CA@PtRu ANPs with an average diameter of 3.2 nm revealed to have enhanced surface area, peroxidase-like activity, high stability, and adequate availability of functional groups to bind biomolecules. Read More

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Gold(III) Aryl Complexes as Reagents for Constructing Hybrid Peptide-Based Assemblies via Cysteine -Arylation.

Inorg Chem 2021 Apr 19;60(7):5054-5062. Epub 2021 Mar 19.

Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.

Organometallic complexes have recently gained attention as competent bioconjugation reagents capable of introducing a diverse array of substrates to biomolecule substrates. Here, we detail the synthesis and characterization of an aminophosphine-supported Au(III) platform that provides rapid and convenient access to a wide array of peptide-based assemblies via cysteine -arylation. This strategy results in the formation of robust C-S covalent linkages and is an attractive method for the modification of complex biomolecules due to the high functional group tolerance, chemoselectivity, and rapid reaction kinetics associated with these arylation reactions. Read More

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Bioorthogonal Labeling and Chemoselective Functionalization of Lung Extracellular Matrix.

Bio Protoc 2021 Feb 20;11(4):e3922. Epub 2021 Feb 20.

Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, USA.

Decellularized extracellular matrix (ECM) biomaterials derived from native tissues and organs are widely used for tissue engineering and wound repair. To boost their regenerative potential, ECM biomaterials can be functionalized via the immobilization of bioactive molecules. To enable ECM functionalization in a chemoselective manner, we have recently reported an effective approach for labeling native organ ECM with the click chemistry-reactive azide ligand via physiologic post-translational glycosylation. Read More

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February 2021

Mechanism of Mesoporous Silica Nanoparticle Interaction with Hairy Root Cultures during Nanoharvesting of Biomolecules.

Adv Biol (Weinh) 2021 03 9;5(3):e2000173. Epub 2021 Feb 9.

Department of Chemical and Materials Engineering, University of Kentucky, 177 F. Paul Anderson Tower, Lexington, KY, 40506, USA.

Cellular uptake and expulsion mechanisms of engineered mesoporous silica nanoparticles (MSNPs) are important in their design for novel biomolecule isolation and delivery applications such as nanoharvesting, defined as using nanocarriers to transport and isolate valuable therapeutics (secondary metabolites) out of living plant organ cultures (e.g., hairy roots). Read More

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New Bifunctional Chelators Incorporating Dibromomaleimide Groups for Radiolabeling of Antibodies with Positron Emission Tomography Imaging Radioisotopes.

Bioconjug Chem 2021 Mar 16. Epub 2021 Mar 16.

School of Biomedical Engineering and Imaging Sciences, King's College London, St. Thomas' Hospital, London SE1 7EH, U.K.

Positron Emission Tomography (PET) imaging with antibody-based contrast agents frequently uses the radioisotopes [Cu]Cu and [Zr]Zr. The macrobicyclic chelator commonly known as sarcophagine (sar) is ideal for labeling receptor-targeted biomolecules with [Cu]Cu. The siderophore chelator, desferrioxamine-B (dfo), has been widely used to incorporate [Zr]Zr into antibodies. Read More

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C-Reactive Protein Sensor Based on Porous Silicon Bragg Stack Interferometers.

J Nanosci Nanotechnol 2021 Jul;21(7):3759-3763

Department of Chemistry, Chosun University, Gwangju 61452, Republic of Korea.

Bio-functionalized nanomaterials represent the cutting-edge research for a sensing biomolecules in nano-systems. Their physicochemical properties of porous silicon bring along advantages in sensing applications. Here, a modified biosensor based on the anti-C-reactive protein-modified porous silicon Bragg stack interferometer was developed to detect C-reactive protein. Read More

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Spherical Nucleic Acid Mediated Functionalization of Polydopamine-Coated Nanoparticles for Selective DNA Extraction and Detection.

Bioconjug Chem 2021 04 12;32(4):801-809. Epub 2021 Mar 12.

Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

Magnetic nanoparticles have been widely used for the separation of biomolecules for biological applications due to the mild and efficient separation process. In previous studies, core-shell magnetic nanoparticles (NPs) were designed for DNA extraction without much sequence specificity. In this work, to achieve highly selective DNA extraction, we designed a core-shell magnetic structure by coating polydopamine (PDA) on FeO NPs. Read More

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Iodosobenzene-Mediated Three-Component Selenofunctionalization of Olefins.

J Org Chem 2021 Apr 11;86(7):5292-5304. Epub 2021 Mar 11.

School of Pharmacy and Analysis & Testing Center, Nantong University, Nantong 226019, People's Republic of China.

A three-component reaction of olefin, diselenide and water, alcohols, phenol, carboxylic acid, or amine by a commercially available hypervalent iodine(III) reagent, PhIO, was developed. This method provides access to a wide range of vicinally functionalized selenoderivatives under ambient conditions with mostly excellent yields and high diastereoselectivity. The developed reaction displays high levels of functional group compatibility and is suitable for the late-stage functionalization of styrene-functionalized biomolecules. Read More

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Quinoline Functionalized Schiff Base Silver (I) Complexes: Interactions with Biomolecules and In Vitro Cytotoxicity, Antioxidant and Antimicrobial Activities.

Molecules 2021 Feb 24;26(5). Epub 2021 Feb 24.

School of Chemistry and Physics, Pietermaritzburg Campus, University of Kwazulu-Natal, Private Bag X01, Scottsville 3209, South Africa.

A series of fifteen silver (I) quinoline complexes - have been synthesized and studied for their biological activities. - were synthesized from the reactions of quinolinyl Schiff base derivatives - (obtained by condensing 2-quinolinecarboxaldehyde with various aniline derivatives) with AgNO, AgClO and AgCFSO. - were characterized by various spectroscopic techniques and the structures of [Ag()]NO, [Ag()]ClO, [Ag()]ClO, [Ag()]CFSO and [Ag()]CFSO were unequivocally determined by single crystal X-ray diffraction analysis. Read More

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February 2021

Natural medicine delivery from biomedical devices to treat bone disorders: A review.

Acta Biomater 2021 May 28;126:63-91. Epub 2021 Feb 28.

W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, United States.

With an increasing life expectancy and aging population, orthopedic defects and bone graft surgeries are increasing in global prevalence. Research to date has advanced the understanding of bone biology and defect repair mechanism, leading to a marked success in the development of synthetic bone substitutes. Yet, the quest for functionalized bone grafts prompted the researchers to find a viable alternative that regulates cellular activity and supports bone regeneration and healing process without causing serious side-effects. Read More

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