65,110 results match your criteria functionality


An SNX10-dependent mechanism downregulates fusion between mature osteoclasts.

J Cell Sci 2021 May 11;134(9). Epub 2021 May 11.

Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel.

Homozygosity for the R51Q mutation in sorting nexin 10 (SNX10) inactivates osteoclasts (OCLs) and induces autosomal recessive osteopetrosis in humans and in mice. We show here that the fusion of wild-type murine monocytes to form OCLs is highly regulated, and that its extent is limited by blocking fusion between mature OCLs. In contrast, monocytes from homozygous R51Q SNX10 mice fuse uncontrollably, forming giant dysfunctional OCLs that can become 10- to 100-fold larger than their wild-type counterparts. Read More

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Physicians' perceptions about narrative note sections format and content: A multi-specialty survey.

Int J Med Inform 2021 May 7;151:104475. Epub 2021 May 7.

Informatics Institute, University of Alabama at Birmingham, AL, USA.

Objective: To assess physicians' perceptions about narrative note sections format and content commonly reported in visit notes to inform future research and EHR development.

Methods: We conducted two online surveys with a multi-specialty panel of outpatient physicians from a large health system to collect their perceptions of the usefulness of three narrative formats and the relevance of content reported in the note sections history of present illness (HPI) and assessment and plan (AP). Survey questions were responded with a 7-point Likert scale and include two open-ended questions for comments on challenges and suggestions related to electronic clinical documentation. Read More

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The multifaceted roles of macrophages in bone regeneration: A story of polarization, activation and time.

Acta Biomater 2021 May 8. Epub 2021 May 8.

Julius Wolff Institut, Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Berlin Institute of Health at Charité - Universitätsmedizin Berlin, BIH Center for Regenerative Therapies (BCRT), Charitéplatz 1, 10117 Berlin, Germany. Electronic address:

To present knowledge, macrophages are found in all tissues of the human body. They are a cell population with high plasticity which come with a multitude of functions which appear to be adapted to the respective tissue niche and micro-environment in which they reside. Bone harbors multiple macrophage subpopulations, with the osteoclasts as classical representative of a bone resorbing cells and osteomacs as a bone tissue resident macrophage first described by the expression of F4/80. Read More

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Subcellular distribution of ezrin/radixin/moesin and their roles in the cell surface localization and transport function of P-glycoprotein in human colon adenocarcinoma LS180 cells.

PLoS One 2021 11;16(5):e0250889. Epub 2021 May 11.

Laboratory of Clinical Pharmaceutics, Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Osaka, Japan.

The ezrin/radixin/moesin (ERM) family proteins act as linkers between the actin cytoskeleton and P-glycoprotein (P-gp) and regulate the plasma membrane localization and functionality of the latter in various cancer cells. Notably, P-gp overexpression in the plasma membrane of cancer cells is a principal factor responsible for multidrug resistance and drug-induced mutagenesis. However, it remains unknown whether the ERM proteins contribute to the plasma membrane localization and transport function of P-gp in human colorectal cancer cells in which the subcellular localization of ERM has yet to be determined. Read More

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5-HT Receptors Are Not Involved in the Effects of Fluoxetine in the Corticosterone Model of Depression.

ACS Chem Neurosci 2021 May 11. Epub 2021 May 11.

Instituto de Biomedicina y Biotecnología de Cantabria, IBBTEC (Universidad de Cantabria, CSIC, SODERCAN), Departamento de Fisiología y Farmacología, Universidad de Cantabria, 39011 Santander, Spain.

Clinical and preclinical studies report the implication of 5-hydroxytryptamine 4 receptors (5-HTRs) in depression and anxiety. Here, we tested whether the absence of 5-HT4Rs influences the response to the antidepressant fluoxetine in mice subjected to chronic corticosterone administration, an animal model of depression and anxiety. Therefore, the effects of chronic administration of fluoxetine in corticosterone-treated wild-type (WT) and 5-HTR knockout (KO) mice were evaluated in the open-field and novelty suppressed feeding tests. Read More

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Visual outcome after rhegmatogenous retinal detachment repair surgery in patients with multifocal versus monofocal intraocular lenses.

J Cataract Refract Surg 2021 Apr 28. Epub 2021 Apr 28.

1Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany 2University Eye Hospital Ulm, Germany.

Purpose: To evaluate functional outcome after retinal detachment (RD) repair surgery in eyes with a multifocal intraocular lens (MIOL).

Setting: Ludwig-Maximilias-University, Munich, Germany DESIGN:: Single-center case control study.

Methods: Fifty-two pseudophakic eyes of 48 patients with successful anatomical outcome after surgical RD repair were included. Read More

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Dimethylacetamide in Rooster Semen Cryopreservation.

Cryo Letters 2021 Jan-Feb;42(1):39-43

Reproducao Animal Comparada, Instituto de Ciencia Biologicas, Universidade Federal de Rio Grande, Rio Grande, Brasil.

Background: Sperm cryopreservation of cockerels is a major challenge, and so far there is no adequate information to enable commercial use of frozen semen.

Objective: To test the toxicity of dimethylacetamide (DMA).

Materials And Methods: DMA was added at 3%, 6%, 9% and 12% to the freezing diluent, and maintained for equilibration with the semen sample for 1 min, 3 min, 5 min, 7 min and 9 min prior to freezing. Read More

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Exogenous ATP in the Cryopreservation of Brycon orbignyanus Spermatozoa.

Cryo Letters 2020 Jan-Feb;41(1):13-18

Federal University of Rio Grande, Biology of Continental Aquatic Environments. Rio Grande; Federal University of Pelotas, Veterinary Medicine. Capao do Leao, Brazil.

Background: ATP exogenous (ATPe) has been used successfully in improving motility and fertility for many animal species. However this has not yet been tested on Brycon orbignyamus.

Objective: The objective of this study was to evaluate the use of ATPe for the cryopreservation of sperm from B. Read More

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Quality Improvement of Post-Thawed Stallion Epididymal Spermatozoa with Single Layer Centrifugation.

Cryo Letters 2020 Jan-Feb;41(1):6-12

Programa de Pos Graduacao Medicina Animal: Equinos, Faculdade de Veterinaria, Universidade Federal do Rio Grande do Sul; Curso de Medicina Veterinaria, Universidade Luterana, Rio Grande do Sul, Brazil.

Background: Epididymal sperm cryopreservation represents the ultimate option to preserve spermatozoa of valuable stallions.

Objective: The study aims to evalute whether single layer centrifugation (SLC) prior to cryopreservation or after post-thawing improves the quality of stallion epididymal sperm.

Materials And Methods: Epididymal sperms of stallions were harvested (N=20). Read More

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Effect of Amide on Semen Cryopreservation of Curimba (Prochilodus lineatus).

Cryo Letters 2020 Jan-Feb;41(1):1-5

Nucleus of Teaching and Research in Animal Reproduction, Federal University of Pelotas, Pelotas, Brazil.

Background: The low molecular weight and high cellular permeability of amides make them suitable for use as penetrative cryoprotectants for sperm cells.

Objective: This study aims to evaluate the effect of dimethylformamide (DMF) and dimethylacetamide (DMA) on sperm cryopreservation of Curimba (Prochilodus lineatus).

Materials And Methods: Semen samples were diluted in media containing cryoprotectants [DMF, DMA and dimethyl sulfoxide (DMSO)]. Read More

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Coordination sphere hydrogen bonding as a structural element in metal-organic Frameworks.

Authors:
Chris S Hawes

Dalton Trans 2021 May 12;50(18):6034-6049. Epub 2021 Apr 12.

School of Chemical and Physical Sciences, Keele University, Keele ST5 5BG, UK.

In the design of new metal-organic frameworks, the constant challenges of framework stability and structural predictability continue to influence ligand choice in favour of well-studied dicarboxylates and similar ligands. However, a small subset of known MOF ligands contains suitable functionality for coordination sphere hydrogen bonding which can provide new opportunities in ligand design. Such interactions may serve to support and rigidity the coordination geometry of mononuclear coordination spheres, as well as providing extra thermodynamic and kinetic stabilisation to meet the challenge of hydrolytic stability in these materials. Read More

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Modeling and Experimental Validation of Algorithms for Maximum Quantity of Protein to be Immobilized on Solid Supports by Electrostatic Adsorption in the Strategy of Rational Design of Immobilized Derivatives.

Protein J 2021 May 10. Epub 2021 May 10.

Centro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25, #455, e/ J e I, Vedado, 10400, Havana, Cuba.

Protein immobilization by electrostatic adsorption to a support could represent a good option. On the other hand, lysozyme (EC 3.2. Read More

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Cell-surface SLC nucleoside transporters and purine levels modulate BRD4-dependent chromatin states.

Nat Metab 2021 May 10. Epub 2021 May 10.

CeMM, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.

Metabolism negotiates cell-endogenous requirements of energy, nutrients and building blocks with the immediate environment to enable various processes, including growth and differentiation. While there is an increasing number of examples of crosstalk between metabolism and chromatin, few involve uptake of exogenous metabolites. Solute carriers (SLCs) represent the largest group of transporters in the human genome and are responsible for the transport of a wide variety of substrates, including nutrients and metabolites. Read More

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Magnetization control by angular momentum transfer from surface acoustic wave to ferromagnetic spin moments.

Nat Commun 2021 May 10;12(1):2599. Epub 2021 May 10.

Institute for Materials Research, Tohoku University, Sendai, 980-8577, Japan.

Interconversion between electron spin and other forms of angular momentum is useful for spin-based information processing. Well-studied examples of this are the conversion of photon angular momentum and rotation into ferromagnetic moment. Recently, several theoretical studies have suggested that the circular vibration of atoms work as phonon angular momentum; however, conversion between phonon angular momentum and spin-moment has yet to be demonstrated. Read More

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Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum.

Biotechnol Biofuels 2021 May 10;14(1):117. Epub 2021 May 10.

Institute of Microbiology and Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.

Background: The interest in using methanol as a substrate to cultivate acetogens increased in recent years since it can be sustainably produced from syngas and has the additional benefit of reducing greenhouse gas emissions. Eubacterium limosum is one of the few acetogens that can utilize methanol, is genetically accessible and, therefore, a promising candidate for the recombinant production of biocommodities from this C1 carbon source. Although several genetic tools are already available for certain acetogens including E. Read More

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Insights into the vertical distribution of the microbiota in steel plant soils with potentially toxic elements and PAHs contamination after 60 years operation: Abundance, structure, co-occurrence network and functionality.

Sci Total Environ 2021 Apr 26;786:147338. Epub 2021 Apr 26.

Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong 266237, China; Suzhou Research Institute, Shandong University, Suzhou, Jiangsu 215123, China. Electronic address:

Both potentially toxic elements (PTEs) and polycyclic aromatic hydrocarbons (PAHs) are widely present in soil contaminated by steel industries. This study assessed the vertical variation (at 20 cm, 40 cm, 60 cm, 80 cm, 120 cm, and 150 cm depth) of bacterial abundance, community structure, functional genes related to PAHs degradation, and community co-occurrence patterns in an old steel plant soils which contaminated by PTEs and PAHs for 60 years. The excessive PAHs and PTEs in steel plant soils were benzo (a) pyrene, benzo (b) fluoranthene, dibenzo (a, h) anthracene, indeno (1,2,3-c, d) pyrene, and lead (Pb). Read More

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Post-translational modifications and antioxidant properties of different therapeutic human serum albumins.

Int J Biol Macromol 2021 May 7. Epub 2021 May 7.

Kedrion S.p.A., Research and Innovation Department, Via di Fondovalle, Loc., Bolognana 55027, Gallicano (LU), Italy.

Human serum albumin (HSA) is widely used for the treatment of diverse clinical conditions to restore plasma volume, manage burns and treat hypoproteinemia.Although the HSA preparations should ideally preserve its functionality, the structural integrity and antioxidant properties of HSA may be compromised as a result of the manufacturing process. The present study examined seven commercially available HSA preparations for clinical use to investigate their post-translational modifications (PTMs) and antioxidant activity, including DPPH radical-scavenging, peroxyl radical antioxidant and metal binding activities, by means of mass spectrometry and Ellman's assay. Read More

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Trends in functional food development with three-dimensional (3D) food printing technology: prospects for value-added traditionally processed food products.

Crit Rev Food Sci Nutr 2021 May 10:1-38. Epub 2021 May 10.

Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Gauteng, South Africa.

One of the recent, innovative, and digital food revolutions gradually gaining acceptance is three-dimensional food printing (3DFP), an additive technique used to develop products, with the possibility of obtaining foods with complex geometries. Recent interest in this technology has opened the possibilities of complementing existing processes with 3DFP for better value addition. Fermentation and malting are age-long traditional food processes known to improve food value, functionality, and beneficial health constituents. Read More

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Rock-Salt MnSSe Nanocubes Assembled on N-Doped Graphene Forming van der Waals Heterostructured Hybrids as High-Performance Anode for Lithium- and Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2021 May 10. Epub 2021 May 10.

Manganese-based chalcogenides would be of latent capacity in serving as anodes for assembling lithium- and/or sodium-ion batteries (LIBs/SIBs) due to their large theoretical capacity, low price, and low-toxicity functionality, while the low electroconductivity and easy agglomeration behavior may impede their technical applications. Here, a solid-state solution of MnSSe nanocubes in rock-salt phase has been synthesized for the first time at a relatively low temperature from the precursors of Mn(II) acetylacetonate with dibenzyl dichalcogens in oleylamine with octadecene, and the MnSSe nanocubes have been assembled with N-doped graphene to form a new kind of heterostructured nanohybrids (shortened as MnSSe/N-G hybrids), which are very potential for the fabrication of metal-ion batteries including LIBs and/or SIBs. Investigations revealed that there have been dense vacancies generated and active sites increased via nonequilibrium alloying of MnS and MnSe into the solid-solution MnSSe nanocubes with segregation and defects achieved in the low-temperature solution synthetic route. Read More

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Biallelic variants in HPDL cause pure and complicated hereditary spastic paraplegia.

Brain 2021 May 10. Epub 2021 May 10.

Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.

Human 4-hydroxyphenylpyruvate dioxygenase-like (HPDL) is a putative iron-containing non-heme oxygenase of unknown specificity and biological significance. We report 25 families containing 34 individuals with neurological disease associated with biallelic HPDL variants. Phenotypes ranged from juvenile-onset pure hereditary spastic paraplegia to infantile-onset spasticity and global developmental delays, sometimes complicated by episodes of neurological and respiratory decompensation. Read More

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Enabling LIFDI-MS measurements of highly air sensitive organometallic compounds: a combined MS/glovebox technique.

Dalton Trans 2021 May 10. Epub 2021 May 10.

Department of Chemistry, Technical University of Munich, Lichtenbergstraße 4, D-85748 Garching, Germany. and Catalysis Research Center, Technical University of Munich, Ernst-Otto-Fischer-Straße 1, D-85748 Garching, Germany.

A new setup combining a ThermoFisher Exactive Plus Orbitrap Mass Spectrometer with a liquid injection field desorption ionization (LIFDI) source directly connected to an inert atmosphere glovebox is presented. The described setup allows for the analysis of very air- and moisture sensitive samples. Furthermore, the soft nature of LIFDI ionization gives access to the molecular ions of fragile molecules. Read More

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A highly stable multifunctional aptamer for enhancing antitumor immunity against hepatocellular carcinoma by blocking dual immune checkpoints.

Biomater Sci 2021 May 10. Epub 2021 May 10.

The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P. R. China. and Fujian Institute of Research on The Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, P. R. China.

T-lymphocytes play a potent role in cancer immunotherapy; while, limited tumor infiltrating lymphocytes (TILs) combined with severe immunosuppression always significantly hinder their antitumor immune responses, especially in solid tumors such as hepatocellular carcinoma (HCC). Here, we prepared a highly stable multifunctional aptamer for strengthening antitumor immunity against HCC solid tumors through a dual immune checkpoint blockade of CTLA-4 and PD-L1. The engineered multifunctional aptamer (termed P1/C4-bi-apt) can block both CTLA-4/B7 and PD-1/PD-L1 signaling pathways and thus enhance the antitumor immune responses. Read More

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Smart scaffolds: shape memory polymers (SMPs) in tissue engineering.

J Mater Chem B 2021 May 10. Epub 2021 May 10.

Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA. and Department of Materials Science and Engineering, Texas A&M University, College Station, TX 77843, USA and Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.

Smart scaffolds based on shape memory polymer (SMPs) have been increasingly studied in tissue engineering. The unique shape actuating ability of SMP scaffolds has been utilized to improve delivery and/or tissue defect filling. In this regard, these scaffolds may be self-deploying, self-expanding, or self-fitting. Read More

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Design, synthesis, and molecular docking of novel 3,5-disubstituted-1,3,4-oxadiazole derivatives as iNOS inhibitors.

Arch Pharm (Weinheim) 2021 May 9:e2000469. Epub 2021 May 9.

Department of Pharmacology, Faculty of Pharmacy, Yeditepe University, Istanbul, Turkey.

To obtain new anti-inflammatory agents, recent studies have aimed to replace the carboxylate functionality of nonsteroidal anti-inflammatory drugs with less acidic heterocyclic bioisosteres like 1,3,4-oxadiazole to protect the gastric mucosa from free carboxylate moieties. In view of these observations, we designed and synthesized a series of 3,5-disubstituted-1,3,4-oxadiazole derivatives as inhibitors of prostaglandin E (PGE ) and NO production with an improved activity profile. As initial screening, and to examine the anti-inflammatory activities of the compounds, the inhibitions of the productions of lipopolysaccharide-induced NO and PGE in RAW 264. Read More

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Spinodal decomposition of chemically fueled polymer solutions.

Soft Matter 2021 May 10. Epub 2021 May 10.

A3BMS Lab, Department of Chemistry, University of Mainz, Duesbergweg 10-14, 55128 Mainz, Germany. and Cluster of Excellence livMatS @ FIT - Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg, Georges-Köhler-Allee 105, 79110 Freiburg, Germany.

Out-of-equilibrium phase transitions driven by dissipation of chemical energy are a common mechanism for morphological organization and temporal programming in biology. Inspired by this, dissipative self-assembly utilizes chemical reaction networks (CRNs) that consume high-energy molecules (chemical fuels) to generate transient structures and functionality. While a wide range of chemical fuels and building blocks are now available for chemically fueled systems, so far little attention has been paid to the phase-separation process itself. Read More

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Investigation of the , and Mutations and Polymorphisms in Iranian Infertile Women with Polycystic Ovary Syndrome (PCOS).

Rep Biochem Mol Biol 2021 Jan;9(4):470-477

Vali-e-Asr Reproductive Health Research center, Tehran University of Medical Sciences, Tehran, Iran.

Background: Polycystic ovary syndrome (PCOS) is the most common cause of ovarian dysfunction associated with infertility, Oligomenorrhea or amenorrhea, hirsutism, acne, and obesity. A large body of evidence unraveled, three major groups of genes play critical roles in underlying PCOS molecular mechanism. The aim of this study is to investigate critical exonic variant of , and and determine the functionality of these mutations in Iranian patients with PCOS. Read More

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January 2021

Functionality of Nanomaterials and its Technological Aspects - Used in Preventing, Diagnosing and Treating COVID-19.

Mater Today Proc 2021 May 4. Epub 2021 May 4.

Ethiopian Technical University (Federal TVET Institute), Addis Ababa, Ethiopia.

COVID-19 (Coronavirus) has severely affected the life of human beings since December 2019. Many difficulties are faced by human beings to prevent the spread of the corona virus. However, this unexpected evolution of COVID-19 has also thrown many challenges to scientists and researchers so as to develop technologies that can be used to combat COVID-19. Read More

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The Significance of Chloroplast NAD(P)H Dehydrogenase Complex and Its Dependent Cyclic Electron Transport in Photosynthesis.

Front Plant Sci 2021 23;12:661863. Epub 2021 Apr 23.

School of Biological Sciences, The University of Western Australia, Perth, WA, Australia.

Chloroplast NAD(P)H dehydrogenase (NDH) complex, a multiple-subunit complex in the thylakoid membranes mediating cyclic electron transport, is one of the most important alternative electron transport pathways. It was identified to be essential for plant growth and development during stress periods in recent years. The NDH-mediated cyclic electron transport can restore the over-reduction in stroma, maintaining the balance of the redox system in the electron transfer chain and providing the extra ATP needed for the other biochemical reactions. Read More

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Allyl Isothiocyanate: A TAS2R38 Receptor-Dependent Immune Modulator at the Interface Between Personalized Medicine and Nutrition.

Front Immunol 2021 20;12:669005. Epub 2021 Apr 20.

Molecular Preventive Medicine, University Medical Center and Faculty of Medicine-University of Freiburg, Freiburg, Germany.

Understanding individual responses to nutrition and medicine is of growing interest and importance. There is evidence that differences in bitter taste receptor (TAS2R) genes which give rise to two frequent haplotypes, TAS2R38-PAV (functional) and TAS2R38-AVI (non-functional), may impact inter-individual differences in health status. We here analyzed the relevance of the TAS2R38 receptor in the regulation of the human immune response using the TAS2R38 agonist allyl isothiocyanate (AITC) from plants. Read More

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Robust Performance of Potentially Functional SNPs in Machine Learning Models for the Prediction of Atorvastatin-Induced Myalgia.

Front Pharmacol 2021 22;12:605764. Epub 2021 Apr 22.

Department of Medicine, Yong Loo Lin School of Medicine, Cardiovascular Research Institute, National University of Singapore, Singapore, Singapore.

Statins can cause muscle symptoms resulting in poor adherence to therapy and increased cardiovascular risk. We hypothesize that combinations of potentially functional SNPs (pfSNPs), rather than individual SNPs, better predict myalgia in patients on atorvastatin. This study assesses the value of potentially functional single nucleotide polymorphisms (pfSNPs) and employs six machine learning algorithms to identify the combination of SNPs that best predict myalgia. Read More

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