51,039 results match your criteria functional assays

PqqE is a new virulence factor that cleaves junctional adhesion molecule A and disrupts gastric epithelial integrity.

Gut Microbes 2021 Jan-Dec;13(1):1-21

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.

infects approximately half of the world's population and is the strongest risk factor for peptic ulcer disease and gastric cancer, representing a major global health concern. persistently colonizes the gastric epithelium, where it subverts the highly organized structures that maintain epithelial integrity. Here, a unique strategy used by to disrupt the gastric epithelial junctional adhesion molecule-A (JAM-A) is disclosed, using various experimental models that include gastric cell lines, primary human gastric cells, and biopsy specimens of infected and non-infected individuals. Read More

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Chemical composition, antimicrobial, antioxidant, and cytotoxic activity of the essential oil from the leaves of Stachys pilifera Benth.

FEMS Microbiol Lett 2021 May 10. Epub 2021 May 10.

Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS-Armand Frappier, Health and Biotechnology Center, Institute of Nutrition and Functional Foods, Laval, QC, Canada.

Stachys pilifera Benth is an endemic species of Stachys family found in Iran with a wide application as an herbal tea. The objective of this research was to evaluate the antimicrobial, antioxidant, and cytotoxic activity of the essential oil from the aerial parts of S. pilifera. Read More

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Immune sensing of mouse polyomavirus DNA by p204 and cGAS DNA sensors.

FEBS J 2021 May 10. Epub 2021 May 10.

Department of Genetics and Microbiology, Faculty of Science, Charles University, Biocev, Vestec, 25250, Czech Republic.

The mechanism by which DNA viruses interact with different DNA sensors and their connection with the activation of interferon (IFN) type I pathway are poorly understood. We investigated the roles of p204 and cGAS sensors during infection with mouse polyomavirus (MPyV). The phosphorylation of IRF3 and STING proteins and the upregulation of IFN-β and MX-1 genes were detected at the time of replication of MPyV genomes in the nucleus. Read More

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Inhibition of Plasmodium falciparum Lysyl-tRNA synthetase via a piperidine-ring scaffold inspired Cladosporin analogues.

Chembiochem 2021 May 9. Epub 2021 May 9.

National Institute of Malaria Research, Director, Sector 8 Dwarka, Dwarka, New Delhi, Delhi 110077, 110077, delhi, INDIA.

Plasmodium falciparum lysyl tRNA synthetase ( Pf KRS) represent a promising therapeutic anti-malarial target. Cladosporin, a tool compound was identified as a selective and potent PfKRS inhibitor but lacks metabolic stability. Here, we report chemical synthesis, biological evaluation and structural characterization of analogues where the tetrahydropyran (THP) frame of cladosporin is replaced with the piperidine ring bearing functional group variations. Read More

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Design of fusion protein for efficient preparation of cyanovirin-n and rapid enrichment of pseudorabies virus.

Biotechnol Lett 2021 May 9. Epub 2021 May 9.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, 430062, China.

Objective: Cyanovirin-N (CVN) is a cyanobacterial protein with potent neutralizing activity against enveloped virus. To achieve the economic and functional production of CVN, the CVN N-terminally fused with CL7(A mutant of the Colicin E7 Dnase) was utilized to improve the solubility and stability of CVN fusion protein (CL7-CVN). Additionally, to improve the detection limit of existing PRV diagnostic assays, CL7-CVN was used for Pseudorabies virus (PRV) enrichment from larger sample volumes. Read More

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KIF11 Serves as an Independent Prognostic Factor and Therapeutic Target for Patients With Lung Adenocarcinoma.

Front Oncol 2021 23;11:670218. Epub 2021 Apr 23.

Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medicine, Jilin University, Changchun, China.

Background: Lung adenocarcinoma (LUAD) is challenging in clinical practice due to the poor understanding of molecular mechanisms and limited therapeutic targets. Herein, the work aimed to use bioinformatics to identify a promising molecular target for LUAD therapy.

Methods: Differentially expressed genes (DEGs) from the Cancer Genome Atlas (TCGA) dataset were used for a weighted gene co-expression network analysis (WGCNA) to screen the hub gene. Read More

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Downregulation of GLYAT Facilitates Tumor Growth and Metastasis and Poor Clinical Outcomes Through the PI3K/AKT/Snail Pathway in Human Breast Cancer.

Front Oncol 2021 22;11:641399. Epub 2021 Apr 22.

Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, China.

Background: The Glycine N-acyltransferase (GLYAT) gene encodes a protein that catalyzes the transfer of acyl groups from acyl CoA to glycine, resulting in acyl glycine and coenzyme A. Aberrant GLYAT expression is associated with several malignant tumors, but its clinical importance in human breast cancer (BC), has yet to be fully addressed. This study aims to evaluate the clinical function of GLYAT in BC patients. Read More

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Krüppel-like factor 5 is upregulated and induces cell proliferation in endometrial cancer.

Oncol Lett 2021 Jun 21;21(6):484. Epub 2021 Apr 21.

Department of Obstetrics and Gynecology, Tokyo Medical University, Shinjuku, Tokyo 160-0023, Japan.

Krüppel-like factor 5 (KLF5) is involved in various cellular processes, such as cell proliferation and survival. KLF5 has been implicated in cancer pathology. The aim of the present study was to investigate the expression levels and function of KLF5 in endometrial cancer. Read More

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Investigating binding tendency, stability of Sulforaphane-N-acetyl cysteine in the active site of histone deacetylase 2 (HDAC2) and testing its cytotoxicity against distinct cancer lines through stringent molecular dynamics, DFT and cell based assays.

Chem Biol Drug Des 2021 May 9. Epub 2021 May 9.

Division of Basic Sciences and Humanities, FoA, SKUAST-Kashmir, 193201, Jammu & Kashmir, India.

Histone deacetylase 2 (HDAC2), an isozyme of Class I HDACs has potential implications in inducing neurodegenerative signalling. Currently there are substantial therapeutic concerns with the use of synthetic histone deacetylase inhibitors in disease management. This strongly suggests the unmet medical need of plant substitutes for therapeutic intervention. Read More

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Silencing periostin inhibits myofibroblast transdifferentiation of fibrotic buccal mucosal fibroblasts.

J Formos Med Assoc 2021 May 5. Epub 2021 May 5.

School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Institute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan. Electronic address:

Background/purpose: Oral submucous fibrosis (OSF) a well-recognized oral premalignant disorder. Several studies have demonstrated that periostin, a matricellular protein, is involved in the development and pathogenesis of fibrosis diseases. Nevertheless, the contribution of periostin in OSF remains to be uncovered. Read More

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Assessment of structural protein expression by FTIR and biochemical assays as biomarkers of metabolites response in gastric and colon cancer.

Talanta 2021 Aug 1;231:122353. Epub 2021 Apr 1.

Institute of Nuclear Physics Polish Academy of Science, 31-342, Krakow, Poland. Electronic address:

Colon and gastric cancers are the widespread benign types of cancers which are synchronous and metachronous neoplasms. In terms of the progression and progress of the disease, metabolic processes and differentiation in protein structures have an important role in for treatment of the disease. In this study we proposed to investigate the metabolic process and the differentiation of protein secondary structure among colon and gastric cancer as well as healthy controls using biochemistry and Fourier Transform InfraRed spectroscopy (FTIR) methods. Read More

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Decidualization modulates the mesenchymal stromal/stem cell and pericyte characteristics of human decidual stromal cells. Effects on antigen expression, chemotactic activity on monocytes and antitumoral activity.

J Reprod Immunol 2021 May 1;145:103326. Epub 2021 May 1.

Instituto de Biopatología y Medicina Regenerativa, Centro de Investigación Biomédica, Universidad de Granada, Armilla, Granada, Spain; Departamento de Bioquímica y Biología Molecular III e Inmunología, Universidad de Granada, Granada, Spain; Unidad de Gestión Clínica Laboratorios, Complejo Hospitalario Universitario de Granada, Granada, Spain.

Decidual stromal cells (DSCs) are the most abundant cellular component of human decidua and play a central role in maternal-fetal immune tolerance. Antigen phenotyping and functional studies recently confirmed the relationship of DSCs with mesenchymal stem/stromal cells (MSCs) and pericytes, the latter two cell types being closely related or identical. The present study investigated the effect of decidualization, a process of cell differentiation driven by progesterone (P4) and other pregnancy hormones, on the MSC/pericyte characteristics of DSCs. Read More

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Enhancing hemocompatibility and the performance of Au@silica nanoparticles by coating with cRGD functionalized zein.

Mater Sci Eng C Mater Biol Appl 2021 Jun 31;125:112064. Epub 2021 Mar 31.

Guangdong Provincial Key Lab of Green Chemical Product Technology, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address:

Poor safety and effectiveness is an outstanding challenge in the preparation of drug delivery systems (DDS) for cancer treatment. The pursuit of the high curative effect will inevitably increase the risk of adverse side effects. Herein, a bio-safe DDS was constructed by combining the advantages of functional zein and Au doped mesoporous silica nanoparticles (Au@SiO) to achieve chemo-photothermal therapy. Read More

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Mitochondrial Ribosomal Small Subunit proteins (MRPS) MRPS6 and MRPS23 show dysregulation in breast cancer affecting tumorigenic cellular processes.

Gene 2021 May 5:145697. Epub 2021 May 5.

Department of Molecular Oncology, Cancer Institute (WIA), Adyar, Chennai, 600020, Tamil Nadu, India.

Human Mitoribosomal Small Subunit unit (MRPS) family of genes appears to have role in cancer. Gene expression analysis of select MRPS genes (n=9) in 15 cancer cell lines showed altered expression in cancer cells. Protein levels of MRPS6, MRPS23 showed significant overexpression in breast cancer cells and tissues. Read More

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Unravelling the mechanisms of Type 1A topoisomerases using single-molecule approaches.

Nucleic Acids Res 2021 May 8. Epub 2021 May 8.

Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK.

Topoisomerases are essential enzymes that regulate DNA topology. Type 1A family topoisomerases are found in nearly all living organisms and are unique in that they require single-stranded (ss)DNA for activity. These enzymes are vital for maintaining supercoiling homeostasis and resolving DNA entanglements generated during DNA replication and repair. Read More

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Integrative analysis of miRNAs and mRNAs revealed regulation of lipid metabolism in dairy cattle.

Funct Integr Genomics 2021 May 7. Epub 2021 May 7.

College of Animal Science, Jilin University, Changchun, 130062, People's Republic of China.

Lipid metabolism in bovine mammary epithelial cells has been the primary focus of the research of milk fat percentage of dairy cattle. Functional microRNAs can affect lipid metabolism by regulating the expression of candidate genes. The purpose of the study was to screen and identify differentially expressed miRNAs, candidate genes, and co-regulatory pathways related to the metabolism of milk fat. Read More

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Selective sweep for an enhancer involucrin allele identifies skin barrier adaptation out of Africa.

Nat Commun 2021 May 7;12(1):2557. Epub 2021 May 7.

Division of Dermatology, Department of Medicine, Washington University in St. Louis School of Medicine, St. Louis, MO, 63110, USA.

The genetic modules that contribute to human evolution are poorly understood. Here we investigate positive selection in the Epidermal Differentiation Complex locus for skin barrier adaptation in diverse HapMap human populations (CEU, JPT/CHB, and YRI). Using Composite of Multiple Signals and iSAFE, we identify selective sweeps for LCE1A-SMCP and involucrin (IVL) haplotypes associated with human migration out-of-Africa, reaching near fixation in European populations. Read More

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ROC1 promotes the malignant progression of bladder cancer by regulating p-IκBα/NF-κB signaling.

J Exp Clin Cancer Res 2021 May 7;40(1):158. Epub 2021 May 7.

Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No.100 Haining Road, Hongkou District, Shanghai, 200080, China.

Background: Regulator of cullins 1 (ROC1) is an important catalytic subunit of cullin-RING E3 ligase. Nuclear factor-kappa B (NF-κB) signaling is closely related to tumor invasion and metastasis. Earlier, we reported that ROC1 was associated with a poor prognosis in patients with bladder cancer (BCa). Read More

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Hematopoietic stem cells retain functional potential and molecular identity in hibernation cultures.

Stem Cell Reports 2021 Apr 27. Epub 2021 Apr 27.

Wellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK; York Biomedical Research Institute, Department of Biology, University of York, York YO10 5DD, UK. Electronic address:

Advances in the isolation and gene expression profiling of single hematopoietic stem cells (HSCs) have permitted in-depth resolution of their molecular program. However, long-term HSCs can only be isolated to near purity from adult mouse bone marrow, thereby precluding studies of their molecular program in different physiological states. Here, we describe a powerful 7-day HSC hibernation culture system that maintains HSCs as single cells in the absence of a physical niche. Read More

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Luminescence lifetime imaging of three-dimensional biological objects.

J Cell Sci 2021 May 7;134(9):1-17. Epub 2021 May 7.

Department of Molecular and Cellular Physiology, Albany Medical College, Albany, NY 12208, USA.

A major focus of current biological studies is to fill the knowledge gaps between cell, tissue and organism scales. To this end, a wide array of contemporary optical analytical tools enable multiparameter quantitative imaging of live and fixed cells, three-dimensional (3D) systems, tissues, organs and organisms in the context of their complex spatiotemporal biological and molecular features. In particular, the modalities of luminescence lifetime imaging, comprising fluorescence lifetime imaging (FLI) and phosphorescence lifetime imaging microscopy (PLIM), in synergy with Förster resonance energy transfer (FRET) assays, provide a wealth of information. Read More

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Effects of the Maillard reaction on the epitopes and immunoreactivity of tropomyosin, a major allergen in .

Food Funct 2021 May 7. Epub 2021 May 7.

College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, Fujian 361021, China.

Scallop (Chlamys nobilis) causes an IgE-mediated food allergy; however, studies of the allergens in its musculus are not sufficiently comprehensive. In this context, the target protein was purified from scallops and confirmed to be the major allergen tropomyosin (TM) using proteomic technology and serological testing. Subsequently, seven potential IgE epitopes of TM were obtained using phage display technology with IgE enrichment from the serum of scallop-sensitized patients and identified via inhibition enzyme-linked immunosorbent assays. Read More

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The bacterial effector HopZ1a acetylates MKK7 to suppress plant immunity.

New Phytol 2021 May 7. Epub 2021 May 7.

Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Depto. Biología Celular, Genética y Fisiología, Campus de Teatinos, Málaga, E-29071, Spain.

The Pseudomonas syringae type III secretion system translocates effector proteins into the host cell cytosol to suppress plant basal immunity. Effector HopZ1a suppresses local and systemic immunity triggered by pathogen-associated molecular patterns (PAMPs) and effectors, through target acetylation. HopZ1a has been shown to target several plant proteins, but none fully substantiates HopZ1a-associated immune suppression. Read More

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STAM Prolongs Clear Cell Renal Cell Carcinoma Patients' Survival Inhibiting Cell Growth and Invasion.

Front Oncol 2021 20;11:611081. Epub 2021 Apr 20.

Department of Urology and Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.

Signal transducing adaptor molecule 1 (STAM1) was considered to mediate cell growth and be involved in multiple signaling pathways; however, no research on the role of STAM1 in any tumors has been published yet. Our study aimed to investigate the prognostic value of STAM1 for clear cell renal cell carcinoma (ccRCC) and its role in modulating cancer cell function. Data from The Cancer Genome Atlas (TCGA) in December 2019 were used to examine the role of STAM1 in indicating ccRCC patients' survival. Read More

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8-Substituted Triazolobenzodiazepines: and Pharmacology in Relation to Structural Docking at the α1 Subunit-Containing GABA Receptor.

Front Pharmacol 2021 20;12:625233. Epub 2021 Apr 20.

Department of Psychiatry and Human Behavior, University of Mississippi Medical Center, Jackson, MS, United States.

In order to develop improved anxiolytic drugs, 8-substituted analogs of triazolam were synthesized in an effort to discover compounds with selectivity for α2/α3 subunit-containing GABA subtypes. Two compounds in this series, XLi-JY-DMH (6-(2-chlorophenyl)-8-ethynyl-1-methyl-4H-benzo [f][1,2,4]triazolo[4,3-a][1,4]diazepine) and SH-TRI-108 [(E)-8-ethynyl-1-methyl-6-(pyridin-2-yl)-4H-benzo [f][1,2,4]triazolo[4,3-a][1,4]diazepine], were evaluated for and properties associated with GABA subtype-selective ligands. In radioligand binding assays conducted in transfected HEK cells containing rat αXβ3γ2 subtypes (X = 1,2,3,5), no evidence of selectivity was obtained, although differences in potency relative to triazolam were observed overall (triazolam > XLi-JY-DMH > SH-TRI-108). Read More

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Effects of CXCL12 isoforms in a pancreatic pre-tumour cellular model: Microarray analysis.

World J Gastroenterol 2021 Apr;27(15):1616-1629

Department of Specialistic Clinical and Odontostomatological Sciences, Polytechnic University of Marche, Ancona 60126, Italy.

Background: Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of death among cancers, it is characterized by poor prognosis and strong chemoresistance. In the PDAC microenvironment, stromal cells release different extracellular components, including CXCL12. The CXCL12 is a chemokine promoting the communication between tumour and stromal cells. Read More

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Integrase-derived peptides together with CD24-targeted lentiviral particles inhibit the growth of CD24 expressing cancer cells.

Oncogene 2021 May 6. Epub 2021 May 6.

Health Promotion Center and Integrated Cancer Prevention Center, Sourasky Medical Center, Tel-Aviv, Israel.

The integration of viral DNA into the host genome is mediated by viral integrase, resulting in the accumulation of double-strand breaks. Integrase-derived peptides (INS and INR) increase the number of integration events, leading to escalated genomic instability that induces apoptosis. CD24 is a surface protein expressed mostly in cancer cells and is very rarely found in normal cells. Read More

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Differential In vitro Pharmacological Profiles of Structurally Diverse Nociceptin Receptor Agonists in Activating G-protein and Beta-arrestin Signaling at the Human Nociceptin Opioid Receptor.

Mol Pharmacol 2021 May 6. Epub 2021 May 6.

Astraea Therapeutics LLC, United States

Agonists at the nociceptin opioid peptide receptor (NOP) are under investigation as therapeutics for non-addicting analgesia, opioid use disorder, Parkinson's disease, and other indications. NOP full and partial agonists have both been of interest, particularly since NOP partial agonists show a reduced propensity for behavioral disruption than NOP full agonists. Here, we investigated the in vitro pharmacological properties of chemically diverse NOP receptor agonists in assays measuring functional activation of the NOP receptor such as GTPgS binding, cAMP inhibition, GIRK activation, phosphorylation, β-arrestin recruitment and receptor internalization. Read More

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Effects of a Multimerized Recombinant Autoantibody Against Amyloid-β.

Neuroscience 2021 May 12;463:355-369. Epub 2021 Apr 12.

Chair of Geriatric Medicine, University Duisburg-Essen, Germany; Department of Neurology, Philipps-University, Marburg, Germany. Electronic address:

Alzheimer's disease (AD) is the most common neurodegenerative disease; thus, the search for a cure or causal therapy has become necessary. Despite intense research on this topic in recent decades, there is no curative therapy up today, and also no disease-modifying treatment has been approved. As promising approach passive immunization strategies have thereby come forth. Read More

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MiR-34a suppression targets Nampt to ameliorate bone marrow mesenchymal stem cell senescence by regulating NAD-Sirt1 pathway.

Stem Cell Res Ther 2021 May 6;12(1):271. Epub 2021 May 6.

The Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, 126 Xin Min Street, Changchun, Jilin Province, People's Republic of China.

Background: Expansion-mediated replicative senescence and age-related natural senescence have adverse effects on mesenchymal stem cell (MSC) regenerative capability and functionality, thus severely impairing the extensive applications of MSC-based therapies. Emerging evidences suggest that microRNA-34a (miR-34a) has been implicated in the process of MSC senescence; however, the molecular mechanisms with regard to how miR-34a influencing MSC senescence remain largely undetermined.

Methods: MiR-34a expression in MSCs was evaluated utilizing RT-qPCR. Read More

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Targeted truncation of the ADAM17 cytoplasmic domain in mice results in protein destabilization and a hypomorphic phenotype.

J Biol Chem 2021 May 3:100733. Epub 2021 May 3.

Physiology, Biophysics and Systems Biology Program, Weill Cornell Medicine, New York, NY, 10021, USA; Arthritis and Tissue Degeneration Program, Hospital for Special Surgery, New York, NY, 10021, USA; Institute for Advanced Study, Technische Universität München, D-85748 Garching, Germany; Department of Medicine, Physiology and Systems Biology, Weill Cornell Medicine, New York, NY, 10021, USA; Department of Biophysics, Physiology and Systems Biology, Weill Cornell Medicine, New York, NY 10021, USA. Electronic address:

A disintegrin and metalloprotease 17 (ADAM17) is a cell-surface metalloprotease that serves as the principle sheddase for Tumor necrosis factor α (TNFα), interleukin-6 receptor (IL-6R) and several ligands of the epidermal growth factor receptor (EGFR), regulating these crucial signaling pathways. ADAM17 activation requires its transmembrane domain, but not its cytoplasmic domain, and little is known about the role of this domain in vivo. To investigate, we used CRISPR-Cas9 to mutate the endogenous Adam17 locus in mice to produce a mutant ADAM17 lacking its cytoplasmic domain (Adam17Δcyto). Read More

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