437,333 results match your criteria functional analysis


Profiling Analysis of Circular RNA and mRNA in Human Temporal Lobe Epilepsy with Hippocampal Sclerosis ILAE Type 1.

Cell Mol Neurobiol 2021 Aug 2. Epub 2021 Aug 2.

Department of Neurosurgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, Heilongjiang, China.

Hippocampal sclerosis (HS) is the most common surgical pathology associated with temporal lobe epilepsy (TLE). However, the cause of TLE with or without HS remains unknown. Our current study aimed to illustrate the essential molecular mechanism that is potentially involved in the pathogenesis of TLE-HS and to shed light on the transcriptional changes associated with hippocampal sclerosis. Read More

View Article and Full-Text PDF

MIR3142HG promotes lipopolysaccharide-induced acute lung injury by regulating miR-450b-5p/HMGB1 axis.

Mol Cell Biochem 2021 Aug 2. Epub 2021 Aug 2.

Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, No.1678 Dongfang Road, Pudong New Area, Shanghai, 200127, China.

The present study aimed to evaluate the potential roles of MIR3142HG, a novel long non-coding RNA (lncRNA) in lipopolysaccharide (LPS)-induced acute lung injury (ALI). ALI was simulated by the treatment of LPS in human pulmonary microvascular endothelial cells (HPMECs). The expression of MIR3142HG, miR-450b-5p and high-mobility group box 1 (HMGB1) was determined by real-time PCR and western blotting. Read More

View Article and Full-Text PDF

Pole position: How plant cells polarize along the axes.

Plant Cell 2021 Aug 2. Epub 2021 Aug 2.

Laboratory of Biochemistry, Wageningen University, Stippeneng 4, 6703WE Wageningen, the Netherlands.

Having a sense of direction is a fundamental cellular trait that can determine cell shape, division orientation, or function, and ultimately the formation of a functional, multicellular body. Cells acquire and integrate directional information by establishing discrete subcellular domains along an axis with distinct molecular profiles, a process known as cell polarization. Insight into the principles and mechanisms underlying cell polarity has been propelled by decades of extensive research mostly in yeast and animal models. Read More

View Article and Full-Text PDF

Direct to Angiography Suite Without Stopping for Computed Tomography Imaging for Patients With Acute Stroke: A Randomized Clinical Trial.

JAMA Neurol 2021 Aug 2. Epub 2021 Aug 2.

Unitat d'Ictus, Hospital Universitari Vall d'Hebron, Barcelona, Spain.

Importance: Direct transfer to angiography suite (DTAS) for patients with suspected large vessel occlusion (LVO) stroke has been described as an effective and safe measure to reduce workflow time in endovascular treatment (EVT). However, it is unknown whether DTAS improves long-term functional outcomes.

Objective: To explore the effect of DTAS on clinical outcomes among patients with LVO stroke in a randomized clinical trial. Read More

View Article and Full-Text PDF

Being sheltered from a demanding everyday life: experiences of the next of kin to people with dementia attending farm-based daycare.

Int J Qual Stud Health Well-being 2021 Dec;16(1):1959497

Vestfold Hospital Trust, Norwegian National Advisory Unit on Ageing and Health, Tønsberg, Norway.

Purpose: Farm-based daycare (FDC) is a type of daycare service for people with dementia. The aim of the present study was to explore the next of kin's experiences with FDC and how the service may affect their daily life.

Methods: The study has a qualitative, descriptive design. Read More

View Article and Full-Text PDF
December 2021

Facile surface functional polyetheretherketone with antibacterial and immunoregulatory activities for enhanced regeneration toward bacterium-infected bone destruction.

Drug Deliv 2021 Dec;28(1):1649-1663

Department of Orthopedic Surgery, Fujian Medical University Union Hospital, Fuzhou, China.

Existing biologically inert or unmodified implants to treat infectious bone defects or osteomyelitis still cannot effectively solve bacterial infection and osseointegration. In this work, a simple co-deposition strategy was developed to modify porous polyetheretherketone (PEEK) with improved antibacterial activity and controllable immunoregulatory ability. After PEEK was treated by HSO to obtain porous PEEK (SPEEK), the self-polymerization of dopamine was operated on SPEEK in the solution of dopamine and gentamicin sulfate (GS) to prepare polydopamine (pDA) and GS layer-modified SPEEK (labeled as SPEEK-pDA-GS). Read More

View Article and Full-Text PDF
December 2021

Biomechanics of adjacent segment after three-level lumbar fusion, hybrid single-level semi-rigid fixation with two-level lumbar fusion.

Comput Methods Biomech Biomed Engin 2021 Aug 2:1-9. Epub 2021 Aug 2.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, P. R. China.

Multi-level spinal fusion has been reported in some cases to lead to adjacent segment disease (ASD) and proximal junctional kyphosis (PJK). The purpose of this study was to demonstrate a polyether-ether-ketone (PEEK) rod fixation system implanted adjacent to a two-level lumbar fusion would have a lower risk of PJK than three-level lumbar fusion, which was investigated by comparing the biomechanical effects on the adjacent level after surgical procedures. Four finite element (FE) models of the lumbar-sacral spine (intact model (INT), L4-S1 fusion model (L4-S1 FUS), L3-S1 fusion model (L3-S1 FUS), and single-level PEEK rod semi-rigid fixation adjacent to L4-S1 fusion model (FUSPRF)) were established. Read More

View Article and Full-Text PDF

Microbial Roles in Dissolved Organic Matter Transformation in Full-Scale Wastewater Treatment Processes Revealed by Reactomics and Comparative Genomics.

Environ Sci Technol 2021 Aug 2. Epub 2021 Aug 2.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, No. 163, Xianlin Avenue, Nanjing 210023, Jiangsu, P. R. China.

Understanding the degradation of dissolved organic matter (DOM) is vital for optimizing DOM control. However, the microbe-mediated DOM transformation during wastewater treatment remains poorly characterized. Here, microbes and DOM along full-scale biotreatment processes were simultaneously characterized using comparative genomics and high-resolution mass spectrometry-based reactomics. Read More

View Article and Full-Text PDF

HLA genetic polymorphism in patients with Coronavirus Disease 2019 in Midwestern United States.

HLA 2021 Aug 2. Epub 2021 Aug 2.

Division of Laboratory and Genomic Medicine, Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.

The experience of individuals with Coronavirus Disease 2019 (COVID-19) ranges from asymptomatic to life threatening multi-organ dysfunction. Specific HLA alleles may affect the predisposition to severe COVID-19 due to their role in presenting viral peptides to launch the adaptive immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this population-based case-control study in the midwestern United States, we performed high-resolution HLA typing of 234 cases hospitalized for COVID-19 in the St. Read More

View Article and Full-Text PDF

An inducible Cldn11-CreER mouse line for selective targeting of lymphatic valves.

Genesis 2021 Aug 2:e23439. Epub 2021 Aug 2.

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

Luminal valves of collecting lymphatic vessels are critical for maintaining unidirectional flow of lymph and their dysfunction underlies several forms of primary lymphedema. Here, we report on the generation of a transgenic mouse expressing the tamoxifen inducible CreER under the control of Cldn11 promoter that allows, for the first time, selective and temporally controlled targeting of lymphatic valve endothelial cells. We show that within the vasculature CLDN11 is specifically expressed in lymphatic valves but is not required for their development as mice with a global loss of Cldn11 display normal valves in the mesentery. Read More

View Article and Full-Text PDF

Identification of the Key Parameters for Horizontal Transition Dipole Orientation in Fluorescent and TADF Organic Light-Emitting Diodes.

Adv Mater 2021 Aug 2:e2100677. Epub 2021 Aug 2.

Organic Semiconductor Centre, SUPA School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK.

In organic light-emitting diodes (OLEDs), horizontal orientation of the emissive transition dipole moment (TDM) can improve light outcoupling efficiency by up to 50% relative to random orientation. Therefore, there have been extensive efforts to identify drivers of horizontal orientation. The aspect ratio of the emitter molecule and the glass-transition temperature (T ) of the films are currently regarded as particularly important. Read More

View Article and Full-Text PDF

IgE epitope analysis of sarcoplasmic-calcium-binding protein, a heat-resistant allergen in .

Food Funct 2021 Aug 2. Epub 2021 Aug 2.

College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, Fujian 361021, China.

Sarcoplasmic-calcium-binding protein (SCP) has been investigated as a novel allergen in Crassostrea angulata. Nevertheless, knowledge of its effector-cell-based allergic relevance and epitopes is limited. In this study, the heat-resistant allergen SCP was able to induce significant upregulation of CD63 and CD203c (p < 0. Read More

View Article and Full-Text PDF

Identification and validation of a novel redox-related lncRNA prognostic signature in lung adenocarcinoma.

Bioengineered 2021 Dec;12(1):4331-4348

Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.

Lung adenocarcinoma (LUAD) is one of the main causes of cancer deaths globally. Redox is emerging as a crucial contributor to the pathophysiology of LUAD, which can be regulated by long non-coding RNAs (lncRNAs). The aim of our research is to identify a novel redox-related lncRNA prognostic signature (redox-LPS) for better prediction of LUAD prognosis. Read More

View Article and Full-Text PDF
December 2021

Novel fabrication of dual nanoparticle loaded-co-polymeric dressing for effective healing efficiency in wound care after fracture surgery.

J Biomater Sci Polym Ed 2021 Aug 1:1-19. Epub 2021 Aug 1.

Orthopaedic Center, the First People's Hospital of Wenling, Wenling, PR China.

In the present study, curcumin loaded chitosan/poly ethylene glycol nanomaterial (CUR loaded CH/PEG/AgNPs) was fabricated and characterized for wound healing efficiency after fracture surgery. The interaction of functional groups and crystal nature were recorded under FTIR and XRD spectrometer and reveals that the stabilization and purity of NPs was mediated by OH/NH groups in chitosan. FESEM showed the presence of spherical and well dispersed particles. Read More

View Article and Full-Text PDF

Understanding the fundamentals of microbial remediation with emphasize on metabolomics.

Prep Biochem Biotechnol 2021 Aug 1:1-13. Epub 2021 Aug 1.

Department of Biosciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India.

The post-genomic tool metabolomics is a great advancement in science and technology which acquires novel strategies and pathways to analyze various biological compounds. Metabolomics aids in retrieving the qualitative and quantitative data from the various biological system. The current review is focused on the application of metabolomics in bioremediation and helps to focus on the xenobiotic compounds which are discharged into the environment and have long term impact. Read More

View Article and Full-Text PDF

Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer.

Bioengineered 2021 Dec;12(1):4201-4216

State Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Jiangsu Women and Children Health Hospital, Nanjing, China.

Endometrial cancer (EC) is the most common gynecological malignancy. Recent studies have uncovered miRNA acted a striking role in predicting the prognosis of multiple tumors. Over 500 EC samples were selected from the Cancer Genome Atlas (TCGA) database. Read More

View Article and Full-Text PDF
December 2021

Development and Validation of an Instrument to Assess Horseback Mobility in Hippotherapy.

Percept Mot Skills 2021 Aug 1:315125211036578. Epub 2021 Aug 1.

Adapted Motor Activity Laboratory, College of Physical Education, University of Brasília, Brasília, Brazil.

Improvement in rider mobility represents an important functional gain for people with disabilities undergoing hippotherapy. However, there is no validated measuring instrument to track and document the rider's progress in riding activities. In this study, we aimed to develop and establish validity evidence for an instrument to assess hippotherapy participants' mobility on horseback. Read More

View Article and Full-Text PDF

Identification of competing endogenous RNA network in laryngeal squamous cell carcinoma.

Oral Dis 2021 Aug 1. Epub 2021 Aug 1.

Department of Otolaryngology, Shenzhen Hospital, Southern Medical University, Shenzhen, China.

Objective: This study was conducted to investigate key lncRNAs involved in ceRNA network associated with laryngeal squamous cell carcinoma (LSCC).

Materials And Methods: Three mRNA datasets, two miRNA datasets and one lncRNA dataset of LSCC were downloaded from GEO database. Following the identification of differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs) in LSCC compared with adjacent tissues, functional enrichment of DEmRNAs was performed. Read More

View Article and Full-Text PDF

Piwi-piRNA complexes induce stepwise changes in nuclear architecture at target loci.

EMBO J 2021 Aug 2:e108345. Epub 2021 Aug 2.

Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.

PIWI-interacting RNAs (piRNAs) are germline-specific small RNAs that form effector complexes with PIWI proteins (Piwi-piRNA complexes) and play critical roles for preserving genomic integrity by repressing transposable elements (TEs). Drosophila Piwi transcriptionally silences specific targets through heterochromatin formation and increases histone H3K9 methylation (H3K9me3) and histone H1 deposition at these loci, with nuclear RNA export factor variant Nxf2 serving as a co-factor. Using ChEP and DamID-seq, we now uncover a Piwi/Nxf2-dependent target association with nuclear lamins. Read More

View Article and Full-Text PDF

Surface electromyography and gamification: Translational research to advance physical rehabilitation.

J Appl Behav Anal 2021 Aug 1. Epub 2021 Aug 1.

Department of Behavior Analysis, University of North Texas.

Many problems that fall in medical domains of inquiry have behavioral components that can be optimized to increase benefits for patients. For example, although surgical intervention is effective in reducing the pain resulting from osteoarthritis of the knee, patients often struggle to regain complete functional use of the joint. In this translational study, we point to some ways in which behavioral contingencies of reinforcement are compromised, making the recovery process more difficult. Read More

View Article and Full-Text PDF

Imbalance Between Prefronto-Thalamic and Sensorimotor-Thalamic Circuitries Associated with Working Memory Deficit in Schizophrenia.

Schizophr Bull 2021 Aug 2. Epub 2021 Aug 2.

Medical Psychological Center, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Background: Thalamocortical circuit imbalance characterized by prefronto-thalamic hypoconnectivity and sensorimotor-thalamic hyperconnectivity has been consistently documented at rest in schizophrenia (SCZ). However, this thalamocortical imbalance has not been studied during task engagement to date, limiting our understanding of its role in cognitive dysfunction in schizophrenia.

Methods: Both n-back working memory (WM) task-fMRI and resting-state fMRI data were collected from 172 patients with SCZ and 103 healthy control subjects (HC). Read More

View Article and Full-Text PDF

A Computational Framework for Dissociating Development-Related from Individually Variable Flexibility in Regional Modularity Assignment in Early Infancy.

Med Image Comput Comput Assist Interv 2020 29;12267:13-21. Epub 2020 Sep 29.

Department of Radiology and Biomedical Research Imaging Center (BRIC), University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Functional brain development in early infancy is a highly dynamic and complex process. Understanding each brain region's topological role and its development in the brain functional connectivity (FC) networks is essential for early disorder detection. A handful of previous studies have mostly focused on how FC network is changing regarding age. Read More

View Article and Full-Text PDF
September 2020

Metagenomic Profile of Microbial Communities in a Drinking Water Storage Tank Sediment after Sequential Exposure to Monochloramine, Free Chlorine, and Monochloramine.

ACS ES T Water 2021 ;1(5):1283-1294

Center for Environmental Solutions & Emergency Response, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268, United States.

Sediment accumulation in drinking water storage facilities may lead to water quality degradation, including biological growth and disinfectant decay. The current research evaluated the microbiome present in a sediment after sequential exposure to monochloramine, free chlorine, and monochloramine. Chemical profiles within the sediment based on microelectrodes showed evidence of nitrification, and monochloramine slowly penetrated the sediment but was not measurable at lower depths. Read More

View Article and Full-Text PDF
January 2021

Degree of Preservation of Neurovascular Bundles in Radical Prostatectomy and Recurrence of Prostate Cancer.

Eur Urol Open Sci 2021 Aug 19;30:25-33. Epub 2021 Jun 19.

Department of Urology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Background: Reports on possible benefits for continence with nerve-sparing (NS) radical prostatectomy have expanded the indications beyond preservation of erectile function. It is unclear whether NS surgery affects oncological outcomes.

Objective: To determine whether the degree of NS during radical prostatectomy influences oncological outcomes. Read More

View Article and Full-Text PDF

Long-term Functional Urinary and Sexual Outcomes in Patients with Anorectal Malformations-A Systematic Review.

Eur Urol Open Sci 2021 Mar 3;25:29-38. Epub 2021 Feb 3.

Department of Surgery, Odense University Hospital, Odense, Denmark.

Context: Long-term urinary and sexual outcomes after repair of anorectal malformations (ARMs) are currently affected by concomitant malformations of the urinary tract and genitalia, sacral anomalies, and the surgical approach. However, the overall prevalence of urinary and sexual dysfunction remains unclear.

Objective: To evaluate the prevalence of urinary and sexual dysfunction in patients aged >10 yr after repair of ARM in infancy. Read More

View Article and Full-Text PDF

WATER versus WATER II 2-Year Update: Comparing Aquablation Therapy for Benign Prostatic Hyperplasia in 30-80-cm and 80-150-cm Prostates.

Eur Urol Open Sci 2021 Mar 31;25:21-28. Epub 2021 Jan 31.

Division of Urology, Centre Hospitalier de l'Université de Montréal, Montreal, Canada.

Background: Surgical options are limited when treating large (>80 cm) prostates for lower urinary tract symptoms (LUTS) due to benign prostatic hyperplasia (BPH). Open simple prostatectomy remains the most common procedure performed for large prostates. There is a need for novel surgical approaches with shorter learning curves and effective treatment. Read More

View Article and Full-Text PDF

Second Primary Cancers After Kidney Cancers, and Kidney Cancers as Second Primary Cancers.

Eur Urol Open Sci 2021 Feb 9;24:52-59. Epub 2021 Jan 9.

Division of Molecular Genetic Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Background: Second primary cancers (SPCs) are increasing due to improving survival in first primary cancers. Previous studies on SPCs in renal cell carcinoma (RCC) have focused on treatment and other risk factors, but data of RCC as an SPC are scarce.

Objective: In this study, we want to elucidate the risk for any SPC after RCC, and in reverse order, for RCC as an SPC after any cancer. Read More

View Article and Full-Text PDF
February 2021

Salvage Magnetic Resonance Imaging-guided Transurethral Ultrasound Ablation for Localized Radiorecurrent Prostate Cancer: 12-Month Functional and Oncological Results.

Eur Urol Open Sci 2020 Dec 25;22:79-87. Epub 2020 Nov 25.

Department of Urology, University of Turku and Turku University Hospital, Turku, Finland.

Background: Up to half of all men who undergo primary radiotherapy for localized prostate cancer (PCa) experience local recurrence.

Objective: To evaluate the safety and early functional and oncological outcomes of salvage magnetic resonance imaging-guided transurethral ultrasound ablation (sTULSA) for men with localized radiorecurrent PCa.

Design Setting And Participants: This prospective, single-center phase 1 study (NCT03350529) enrolled men with biopsy-proven localized PCa recurrence after radiotherapy. Read More

View Article and Full-Text PDF
December 2020

Living with Bladder Cancer: Self-reported Changes in Patients' Functional and Overall Health Status Following Diagnosis.

Eur Urol Open Sci 2020 Jul 31;20:14-19. Epub 2020 Jul 31.

Department of Surgery-Urology Division, University of Missouri, Columbia, MO, USA.

Background: Bladder cancer affects life quality, overall health, and mortality negatively. The effect of bladder cancer on activities of daily living (ADLs) is not well established.

Objective: To examine the effect of bladder cancer diagnosis on ADLs, in addition to physical, mental, and overall health measures. Read More

View Article and Full-Text PDF

A Systematic Review and Meta-analysis of Prostatic Urethral Lift for Male Lower Urinary Tract Symptoms Secondary to Benign Prostatic Hyperplasia.

Eur Urol Open Sci 2020 Jul 4;19:3-15. Epub 2020 Jun 4.

Department of Urology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Context: Recently, prostatic urethral lift (PUL) is being used to treat lower urinary tract symptoms (LUTS) secondary to benign prostatic hyperplasia (BPH). Although preliminary clinical studies on PUL are increasing, the long-term efficacy and safety of this procedure are still not well evaluated.

Objective: The objective of our study is to synthesize the existing literature evidence, and make a comprehensive and long-term systematic review for the PUL procedure. Read More

View Article and Full-Text PDF