7,657 results match your criteria function podocyte

Kidney damage from nonsteroidal anti-inflammatory drugs-Myth or truth? Review of selected literature.

Pharmacol Res Perspect 2021 Aug;9(4):e00817

Department of Pharmacokinetics and Monitored Therapy, Pomeranian Medical University, Szczecin, Poland.

Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely available drugs with anti-inflammatory and analgesic properties. Their mechanism of action is associated with the enzymes of the arachidonic acid cycle (cyclooxygenases: COX-1 and COX-2). The cyclooxygenase pathway results in the formation of prostanoids (prostaglandins [PGs], prostacyclins, and thromboxanes). Read More

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Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species.

Int J Mol Sci 2021 Jul 15;22(14). Epub 2021 Jul 15.

Department of Physiology, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

Doxorubicin (DOX), a category D pregnancy drug, is a chemotherapeutic agent that has been shown in animal studies to induce fetal toxicity, including renal abnormalities. Upregulation of the transient receptor potential cation (TRPC) 6 channel is involved in DOX-induced podocyte apoptosis. We have previously reported that TRPC6-mediated Ca signaling promotes neonatal glomerular mesangial cell (GMC) death. Read More

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Preparation of single-cell suspensions of mouse glomeruli for high-throughput analysis.

Nat Protoc 2021 Jul 19. Epub 2021 Jul 19.

Department of Research Biology, Genentech, South San Francisco, CA, USA.

The kidney glomerulus is essential for proper kidney function. Until recently, technical challenges associated with glomerular isolation and subsequent dissolution into single cells have limited the detailed characterization of cells in the glomerulus. Previous techniques of kidney dissociation result in low glomerular cell yield, which limits high-throughput analysis. Read More

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The AGE receptor, OST48 drives podocyte foot process effacement and basement membrane expansion (alters structural composition).

Endocrinol Diabetes Metab 2021 Jul 22;4(3):e00278. Epub 2021 Jun 22.

Glycation and Diabetes Complications Mater Research Institute - The University of Queensland Translational Research Institute Woolloongabba Qld Australia.

Aims: The accumulation of advanced glycation end products is implicated in the development and progression of diabetic kidney disease. No study has examined whether stimulating advanced glycation clearance via receptor manipulation is reno-protective in diabetes. Podocytes, which are early contributors to diabetic kidney disease and could be a target for reno-protection. Read More

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Advanced-Glycation End-Products Induce Podocyte Injury and Contribute to Proteinuria.

Front Med (Lausanne) 2021 1;8:685447. Epub 2021 Jul 1.

Department of Biochemistry, University of Hyderabad, Hyderabad, India.

The prevalence of diabetes reaches epidemic proportions. Diabetes is the leading cause of end-stage kidney disease (ESKD) since 30-40% of diabetic patients develop diabetic nephropathy. Albuminuria and glomerular filtration rate used to assess kidney function are considered surrogate outcomes of chronic kidney disease. Read More

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Beneficial effects of metformin on glomerular podocytes in diabetes.

Biochem Pharmacol 2021 Jul 16;192:114687. Epub 2021 Jul 16.

Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Institute Polish Academy of Sciences, Wita Stwosza 63, Gdansk 80-308, Poland; Department of Molecular Biotechnology, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, Gdansk 80-308, Poland. Electronic address:

Podocytes and their foot processes form an important cellular layer of the glomerular barrier involved in regulating glomerular permeability. Disturbances in podocyte function play a central role in the development of proteinuria in diabetic nephropathy. The retraction of podocyte foot processes forming a slit diaphragm is a common feature of proteinuria. Read More

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Nephrotic syndrome-associated mutation of KANK2 induces pathologic binding competition with physiological interactor KIF21A.

J Biol Chem 2021 Jul 15:100958. Epub 2021 Jul 15.

Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China; Academy for Advanced Interdisciplinary Studies, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China; Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, and Shenzhen Key Laboratory of Cell Microenvironment, Shenzhen, Guangdong 518055, China. Electronic address:

Nephrotic syndrome (NS) is a common kidney disorder caused by dysfunction of the glomerular filtration barrier. Some genetic mutations identified in NS patients cause amino acid substitutions of kidney ankyrin repeat-containing (KANK) proteins, which are scaffold proteins that regulate actin polymerization, microtubule targeting and cell adhesion via binding to various molecules, including the kinesin motor protein KIF21A. However, the mechanisms by which these mutations lead to NS are unclear. Read More

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The glomerular filtration barrier: a structural target for novel kidney therapies.

Nat Rev Drug Discov 2021 Jul 14. Epub 2021 Jul 14.

Research and Development, Prime Medicine, Cambridge, MA, USA.

Loss of normal kidney function affects more than 10% of the population and contributes to morbidity and mortality. Kidney diseases are currently treated with immunosuppressive agents, antihypertensives and diuretics with partial but limited success. Most kidney disease is characterized by breakdown of the glomerular filtration barrier (GFB). Read More

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The ketone body β-hydroxybutyrate mitigates the senescence response of glomerular podocytes to diabetic insults.

Kidney Int 2021 Jul 8. Epub 2021 Jul 8.

Division of Nephrology, University of Toledo College of Medicine, Toledo, Ohio;; Division of Kidney Disease and Hypertension, Rhode Island Hospital, Brown Medical School, Providence, Rhode Island;; Department of Medicine, University of Toledo College of Medicine, Toledo, Ohio; Deaprtment of Physiology and Pharmacology, University of Toledo College of Medicine, Toledo, Ohio. Electronic address:

Diabetic kidney disease (DKD) is one of the most common complications of diabetes and clinically featured by progressive albuminuria, consequent to glomerular destruction that involves podocyte senescence. Burgeoning evidence suggests that ketosis, in particular β-hydroxybutyrate, exerts a beneficial effect on aging and on myriad metabolic or chronic diseases, including obesity, diabetes and chronic kidney diseases. Its effect on DKD is largely unknown. Read More

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A study of altered calcium sensing system caused primary membranous nephropathy to end-stage renal failure.

Biomed Pharmacother 2021 Jul 7;141:111777. Epub 2021 Jul 7.

Division of Nephrology, Affiliated Hospital of Chengde Medical University, Chengde, Hebei, China.

PMN (primary membranous nephropathy) is the most prevalent source of idiopathic nephrotic syndrome, which further progressed to ESRD (end-stage renal disease) in non-diabetic adults worldwide. Autoantibodies circulating against podocyte membrane proteins PLA2R1 and THSD7A are present in approximately 75-80% of incidents. Furthermore, a research presented an unusual case of IgG4-RD correlated with elevated serum levels of calcium concluded that renal irregularities have been preceded and triggered by hypercalcemia. Read More

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Pyrroloquinoline quinone (PQQ) alleviated sepsis-induced acute liver injury, inflammation, oxidative stress and cell apoptosis by downregulating CUL3 expression.

Bioengineered 2021 Dec;12(1):2459-2468

Department of Critical Care Medicine, The People's Hospital of Xishuangbanna Dai Nationality Autonomous Prefecture, Jinghong, Yunnan Province, China.

PQQ has anti-inflammatory and anti-oxidant effects. PQQ can relieve high glucose-induced renal cell damage by suppressing Keap1 expression. Keap1 can interact with CUL3. Read More

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December 2021

CCL24 Protects Renal Function by Controlling Inflammation in Podocytes.

Dis Markers 2021 16;2021:8837825. Epub 2021 Jun 16.

Department of Endocrinology and Genetic Metabolism, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu 241002, China.

Diabetic nephropathy (DN) is one of the most lethal complications of diabetes mellitus with chronic inflammation. We have examined the role of the inflammatory chemokine CCL24 in DN. We observed that serum levels of CCL24 were significantly elevated in patients with DN. Read More

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High-Throughput Screening to Identify Small Molecules That Selectively Inhibit APOL1 Protein Level in Podocytes.

SLAS Discov 2021 Jul 3:24725552211026245. Epub 2021 Jul 3.

Screening, Target and Compound Profiling, Merck & Co., Inc., Kenilworth, NJ, USA.

High-throughput phenotypic screening is a key driver for the identification of novel chemical matter in drug discovery for challenging targets, especially for those with an unclear mechanism of pathology. For toxic or gain-of-function proteins, small-molecule suppressors are a targeting/therapeutic strategy that has been successfully applied. As with other high-throughput screens, the screening strategy and proper assays are critical for successfully identifying selective suppressors of the target of interest. Read More

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Engineered nanoplex mediated targeted miRNA delivery to rescue dying podocytes in diabetic nephropathy.

Int J Pharm 2021 Jul 1;605:120842. Epub 2021 Jul 1.

National Institute of Pharmaceutical Education and Research-Ahmedabad (NIPER-A), An Institute of National Importance, Government of India, Department of Pharmaceuticals, Ministry of Chemicals and Fertilizers, Palaj, Opp. Air Force Station, Gandhinagar 382355, Gujarat, India. Electronic address:

MicroRNAs (miRNA) is vital for gene expression regulation and normal kidney function. Mainly, miRNA-30a is responsible for the homeostasis of podocytes. In the diabetic nephropathic condition, miRNA-30a is directly and primarily suppressed by hyperglycemic kidney induced Notch signaling pathway leads to podocyte damage and apoptosis. Read More

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Carnosine, Small but Mighty-Prospect of Use as Functional Ingredient for Functional Food Formulation.

Antioxidants (Basel) 2021 Jun 28;10(7). Epub 2021 Jun 28.

Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, J. Huttlera 4, HR-31000 Osijek, Croatia.

Carnosine is a dipeptide synthesized in the body from β-alanine and L-histidine. It is found in high concentrations in the brain, muscle, and gastrointestinal tissues of humans and is present in all vertebrates. Carnosine has a number of beneficial antioxidant properties. Read More

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Podocalyxin in Normal Tissue and Epithelial Cancer.

Cancers (Basel) 2021 Jun 8;13(12). Epub 2021 Jun 8.

Implantation and Pregnancy Research Laboratory, School of Health and Biomedical Sciences, RMIT University, Bundoora 3083, Australia.

Podocalyxin (PODXL), a glycosylated cell surface sialomucin of the CD34 family, is normally expressed in kidney podocytes, vascular endothelial cells, hematopoietic progenitors, mesothelium, as well as a subset of neurons. In the kidney, PODXL functions primarily as an antiadhesive molecule in podocyte epithelial cells, regulating adhesion and cell morphology, and playing an essential role in the development and function of the organ. Outside the kidney, PODXL plays subtle roles in tissue remodelling and development. Read More

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Simulations of increased glomerular capillary wall strain in the 5/6-nephrectomized rat.

Microcirculation 2021 Jun 30:e12721. Epub 2021 Jun 30.

Department of Physiology, Tulane School of Medicine, New Orleans, LA, USA.

Objective: Chronic glomerular hypertension is associated with glomerular injury and sclerosis; however, the mechanism by which increases in pressure damage glomerular podocytes remains unclear. We tested the hypothesis that increases in glomerular pressure may deleteriously affect podocyte structural integrity by increasing the strain of the glomerular capillary walls, and that glomerular capillary wall strain may play a significant role in the perpetuation of glomerular injury in disease states that are associated with glomerular hypertension.

Methods: We developed an anatomically accurate mathematical model of a compliant, filtering rat glomerulus to quantify the strain of the glomerular capillary walls in a remnant glomerulus of the 5/6-nephrectomized rat model of chronic kidney disease. Read More

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[Renal failure and renal symptoms associated with molecular targeted therapies in oncology].

Rev Prat 2021 Feb;71(2):198-205

"Université Paris Est-Créteil, Inserm, Institut Mondor de recherche biomédicale, Créteil, France".

"Targeted therapies and pathophysiological mechanisms of proteinuria Targeted therapy represents a promising therapeutic approach for patients with diverse cancers and has enabled significant development in medical oncology. This new class of anticancer drugs includes antibodies, fusion-proteins and receptor tyrosine kinase inhibitors among others. Depending on their molecular targeting, side effects can affect multiple organs, especially the kidney. Read More

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February 2021

Effect of Interleukin-17 gene on glomerular ultrastructure and podocyte injury in adriamycin nephropathy rat models.

J Biol Regul Homeost Agents 2021 May-Jun;35(3):1001-1010

Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, Xian, Shaanxi, China.

The aim of this study was to investigate the mechanism of interleukin-17 (IL-17) gene in renal tissues of rats suffering from adriamycin (ADM) nephropathy and its effect on the expression level of characteristic proteins, such as Podocalyxin and Nephrin, in podocytes. Sprague-Dawley (SD) rats were randomly divided into a control group (treated with normal saline) and an ADM group (treated with adriamycin). ADM model rats were transfected with lentivirus and divided into a transfection group (transfected with recombinant plasmid IL-17-shRNA) and a negative control group (transfected with plasmid shNC). Read More

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ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte.

Ren Fail 2021 Dec;43(1):968-979

Department of Nephrology, People's Hospital of Hangzhou Medical College, Zhejiang Provincial People's Hospital, Zhejiang, P.R. China.

Aim: Podocyte injury plays an important role in diabetic nephropathy (DN), yet the underlying molecular mechanisms of podocyte injury in DN is not clear. Here, we investigated the role of activating transcription factor 4 (ATF4) and HO-1 in DN-induced podocyte injury.

Methods: Protein expression was measured by western blotting (WB) and immunofluorescence. Read More

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December 2021

Super-resolved local recruitment of CLDN5 to filtration slits implicates a direct relationship with podocyte foot process effacement.

J Cell Mol Med 2021 Jun 22. Epub 2021 Jun 22.

Department of Anatomy and Cell Biology, University Medicine Greifswald, Greifswald, Germany.

Under healthy conditions, foot processes of neighbouring podocytes are interdigitating and connected by an electron-dense slit diaphragm. Besides slit diaphragm proteins, typical adherens junction proteins are also found to be expressed at this cell-cell junction. It is therefore considered as a highly specialized type of adherens junction. Read More

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Modeling the Glomerular Filtration Barrier and Intercellular Crosstalk.

Front Physiol 2021 2;12:689083. Epub 2021 Jun 2.

Division of Nephrology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.

The glomerulus is a compact cluster of capillaries responsible for blood filtration and initiating urine production in the renal nephrons. A trilaminar structure in the capillary wall forms the glomerular filtration barrier (GFB), composed of glycocalyx-enriched and fenestrated endothelial cells adhering to the glomerular basement membrane and specialized visceral epithelial cells, podocytes, forming the outermost layer with a molecular slit diaphragm between their interdigitating foot processes. The unique dynamic and selective nature of blood filtration to produce urine requires the functionality of each of the GFB components, and hence, mimicking the glomerular filter has been challenging, though critical for various research applications and drug screening. Read More

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PPARγ Mediates the Anti-Epithelial-Mesenchymal Transition Effects of FGF1 in Chronic Kidney Diseases via Inhibition of TGF-β1/SMAD3 Signaling.

Front Pharmacol 2021 3;12:690535. Epub 2021 Jun 3.

The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Podocytes are essential components of the glomerular basement membrane. Epithelial-mesenchymal-transition (EMT) in podocytes results in proteinuria. Fibroblast growth factor 1 (FGF1) protects renal function against diabetic nephropathy (DN). Read More

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Renal effects of growth hormone in health and in kidney disease.

Pediatr Nephrol 2021 Aug 18;36(8):2511-2530. Epub 2021 Jun 18.

Department of Pediatric Kidney, Liver and Metabolic Diseases, Pediatric Research Center, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.

Growth hormone (GH) and its mediator insulin-like growth factor-1 (IGF-1) have manifold effects on the kidneys. GH and IGF receptors are abundantly expressed in the kidney, including the glomerular and tubular cells. GH can act either directly on the kidneys or via circulating or paracrine-synthesized IGF-1. Read More

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Characterization of purinergic receptor 2 signaling in podocytes from diabetic kidneys.

iScience 2021 Jun 11;24(6):102528. Epub 2021 May 11.

Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.

Growing evidence suggests that renal purinergic signaling undergoes significant remodeling during pathophysiological conditions such as diabetes. This study examined the renal P2 receptor profile and ATP-mediated calcium response from podocytes in glomeruli from kidneys with type 1 or type 2 diabetic kidney disease (DKD), using type 2 diabetic nephropathy (T2DN) rats and streptozotocin-injected Dahl salt-sensitive (type 1 diabetes) rats. A dramatic increase in the ATP-mediated intracellular calcium flux in podocytes was observed in both models. Read More

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Lack of APOL1 in proximal tubules of normal human kidneys and proteinuric APOL1 transgenic mouse kidneys.

PLoS One 2021 17;16(6):e0253197. Epub 2021 Jun 17.

Department of Inflammation & Immunity, Cleveland Clinic, Case Western Reserve University School of Medicine, Cleveland, OH, United States of America.

The mechanism of pathogenesis associated with APOL1 polymorphisms and risk for non-diabetic chronic kidney disease (CKD) is not fully understood. Prior studies have minimized a causal role for the circulating APOL1 protein, thus efforts to understand kidney pathogenesis have focused on APOL1 expressed in renal cells. Of the kidney cells reported to express APOL1, the proximal tubule expression patterns are inconsistent in published reports, and whether APOL1 is synthesized by the proximal tubule or possibly APOL1 protein in the blood is filtered and reabsorbed by the proximal tubule remains unclear. Read More

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Rho GTPases in kidney physiology and diseases.

Small GTPases 2021 Jun 17:1-21. Epub 2021 Jun 17.

Faculté De Médecine Et De Pharmacie, Université De Poitiers, Poitiers, France.

Rho family GTPases are molecular switches best known for their pivotal role in dynamic regulation of the actin cytoskeleton, but also of cellular morphology, motility, adhesion and proliferation. The prototypic members of this family (RhoA, Rac1 and Cdc42) also contribute to the normal kidney function and play important roles in the structure and function of various kidney cells including tubular epithelial cells, mesangial cells and podocytes. The kidney's vital filtration function depends on the structural integrity of the glomerulus, the proximal portion of the nephron. Read More

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Lupus Nephritis and Hydroxychloroquine-Associated Zebra Bodies: Not Just in Fabry Disease.

Kidney Med 2021 May-Jun;3(3):442-446. Epub 2021 Mar 18.

Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.

Zebra bodies in kidney biopsy specimens are widely accepted as a specific feature of Fabry disease but they can also be present in a drug-induced mimic of Fabry disease, phospholipidosis. Chloroquine and hydroxychloroquine may both induce zebra body formation and kidney phospholipidosis. However, the frequency and clinical significance of such changes remain unknown. Read More

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Losartan Protects Podocytes against High Glucose-induced Injury by Inhibiting B7-1 Expression.

Curr Med Sci 2021 Jun 15;41(3):505-512. Epub 2021 Jun 15.

Department of Nephrology, Zibo Central Hospital, Zibo, 255036, China.

The role of B7-1 in podocyte injury has received increasing attention. The aim of this study was to investigate whether losartan protects podocytes of patients with diabetic kidney disease (DKD) by regulating B7-1 and the underlying mechanisms. Rats with streptozotocin-induced DKD were treated with losartan for 8 weeks. Read More

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Epigenetic regulation of TXNIP-mediated oxidative stress and NLRP3 inflammasome activation contributes to SAHH inhibition-aggravated diabetic nephropathy.

Redox Biol 2021 09 5;45:102033. Epub 2021 Jun 5.

Guangdong Provincial Key Laboratory of Digestive Cancer Research, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China. Electronic address:

S-adenosylhomocysteine (SAH) is hydrolyzed by SAH hydrolase (SAHH) to homocysteine and adenosine. Increased plasma SAH levels were associated with disturbed renal function in patients with diabetes. However, the role and mechanism of SAHH in diabetic nephropathy is still unknown. Read More

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September 2021