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LncRNA OIP5-AS1 Signatures as a Biomarker of Gestational Diabetes Mellitus and a Regulator on Trophoblast Cells.

Authors:
Yanmei Li Lei Liu

Gynecol Obstet Invest 2021 Nov 29:1-9. Epub 2021 Nov 29.

Department of Obstetrics, Dongying People's Hospital, Dongying, China.

Objectives: Gestational diabetes mellitus (GDM) is a common disorder in pregnant women. Long noncoding RNA (lncRNA) is a fundamental mediator in the pathogenesis of GDM. The study aimed to detect the clinical importance of lncRNA OIP5-AS1 and its underlying regulation on trophoblast cells. Read More

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November 2021

Quercetin regulates inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells by promoting PVT1 expression.

Acta Histochem 2021 Nov 26;123(8):151819. Epub 2021 Nov 26.

Department of Cardiology, Wuhan Third Hospital & Tongren Hospital of Wuhan University, China. Electronic address:

Objective: To investigate the effect and potential mechanism of quercetin on inflammation, oxidative stress, apoptosis, and mitochondrial structure and function in H9C2 cells.

Materials And Methods: H9C2 cells were obtained from the Shanghai Institutes for Biological Sciences, Chinese Academy of Science, and randomly divided into six groups: control, model, PVT1 overexpression (OV), quercetin, OV + quercetin, and NAC groups. The CCK-8 assay was performed to examine cell proliferation. Read More

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November 2021

Comparative analysis of physiological, agronomic and transcriptional responses to drought stress in wheat local varieties from Mongolia and Northern China.

Plant Physiol Biochem 2021 Nov 17;170:23-35. Epub 2021 Nov 17.

Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. Electronic address:

Drought is one of the major abiotic stresses that threaten wheat production worldwide, especially in the Mongolian Plateau and adjacent regions. This study aims to find local wheat varieties with high yields and drought resistance at various developmental stages based on agronomic traits and drought resistance indices analysis and explore the underlining molecular mechanisms by transcriptome analysis. Our results revealed that drought stress started at the seedling stage has a greater impact on crop yields. Read More

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November 2021

Knockdown of optineurin controls C2C12 myoblast differentiation via regulating myogenin and MyoD expressions.

Differentiation 2021 Nov 25;123:1-8. Epub 2021 Nov 25.

Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, 734-8551, Japan.

Mutations in optineurin (OPTN) have been identified in a small proportion of sporadic and familial amyotrophic lateral sclerosis (ALS) cases. Recent evidences suggest that OPTN would be involved in not only the pathophysiological mechanisms of motor neuron death of ALS but also myofiber degeneration of sporadic inclusion body myositis. However, the detailed role of OPTN in muscle remains unclear. Read More

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November 2021

Disordered regions tune order in chromatin organization and function.

Biophys Chem 2021 Nov 17;281:106716. Epub 2021 Nov 17.

Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India. Electronic address:

Intrinsically disordered proteins or hybrid proteins with ordered domains and disordered regions (both collectively designated as IDP(R)s) defy the well-established structure-function paradigm due to their ability to perform multiple biological functions even in the absence of a well-defined 3D structure. IDP(R)s have a unique ability to exist as a functional heterogeneous ensemble, where they adopt multiple thermodynamically stable conformations with low energy barriers between states. The resultant structural plasticity or conformational adaptability provides them with a high functional diversity and ease of regulation. Read More

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November 2021

Conditional Gene Targeting Using UCP1-Cre Mice Directly Targets the Central Nervous System Beyond Thermogenic Adipose Tissues.

Mol Metab 2021 Nov 26:101405. Epub 2021 Nov 26.

Department of Neuroscience and Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Fraternal Order of Eagles Diabetes Research Center, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Iowa Neuroscience Institute, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Department of Veterans Affairs Medical Center, Iowa City, IA, 52242, USA. Electronic address:

Objective: Uncoupling protein 1 (UCP1) is a mitochondrial protein critical for adaptive thermogenesis in adipose tissues, and as such, is typically believed to be restricted to thermogenic adipose tissues. UCP1-Cre transgenic mice are utilized in numerous studies to provide "brown adipose-specific" conditional gene targeting. Here, we examine the distribution of Cre and UCP1 throughout the body in UCP1-Cre reporter mice. Read More

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November 2021

Plasmacytoid dendritic cells mediate the tolerogenic effect of CD8regulatory T cells in a rat tolerant liver transplantation model.

Transpl Immunol 2021 Nov 26:101508. Epub 2021 Nov 26.

Department of Hepatobiliary and Pancreaticosplenic Surgery, Beijing ChaoYang Hospital, Capital Medical University, Beijing 100020, China. Electronic address:

Background: Tolerance is more easily induced in liver transplant models than in other organs; CD8CD45RCregulatory T cells (Tregs) have been shown to induce tolerance in heart allografts. Whether CD8CD45RCTregs could induce tolerance in a liver transplant model and how dendritic cells (DCs) mediate the CD8CD45RCTregs effect remains to be investigated.

Methods: A rat liver transplantation model was established and used to test tolerance and acute rejection compared to control groups. Read More

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November 2021

Slc39a4 in the small intestine predicts zinc absorption and utilization: a comprehensive analysis of zinc transporter expression in response to diets of varied zinc content in young mice.

J Nutr Biochem 2021 Nov 26:108927. Epub 2021 Nov 26.

U.S. Army Research Institute of Environmental Medicine (USARIEM), Military Nutrition Division, Natick, MA, USA.

Zinc homeostasis is primarily maintained by zinc transporters that regulate zinc uptake and efflux in the small intestine; however, the relative contribution of the many zinc transporters identified (Slc39a1-14, Slc30a1-10) to dietary zinc absorption and utilization remains unknown. The objective of this study was to determine the expression of Slc39a1-14 and Slc30a1-10 in the small intestine and their relative contribution to dietary zinc absorption in mice. Five-week-old male C57BL/6J mice were fed modified AIN-93G diets containing <1, 30, or 100ppm zinc (n=15 mice/diet). Read More

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November 2021

Role of EZH2 in Bone Marrow Mesenchymal Stem Cells and Immune-Cancer Interactions.

Crit Rev Oncol Hematol 2021 Nov 26:103547. Epub 2021 Nov 26.

Department of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin, 300052, PR China. Electronic address:

In recent years, methylation modification has been determined to be vital for the biological regulation of normal cells, tumor cells, and tumor microenvironment immune cells. Enhancer of zeste homology 2 (EZH2), a component of the Polycomb Repressive Complex 2 (PRC2), catalyzes the trimethylation of the downstream gene in the tri-methylates histone three lysine 27 (H3K27me3) position, which causes chromatin pyknosis, and thus, silences the expression of related genes. In this paper, we reviewed the role of EZH2 in regulating bone marrow mesenchymal stem cell differentiation and the immune cell function in tumor microenvironment, summarized all types of existing EZH2 inhibitors and the main clinical trials, and proposed relevant ideas for potential clinical applications. Read More

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November 2021

Discovery of sterically-hindered phenol compounds with potent cytoprotective activities against ox-LDL-induced retinal pigment epithelial cell death as a potential pharmacotherapy.

Free Radic Biol Med 2021 Nov 26. Epub 2021 Nov 26.

Harvard Ophthalmology, Schepens Eye Research Institute of Massachusetts Eye and Ear, Boston, MA, USA. Electronic address:

Late-stage dry age-related macular degeneration (AMD) or geographic atrophy (GA) is an irreversible blinding condition characterized by degeneration of retinal pigment epithelium (RPE) and the associated photoreceptors. Clinical and genetic evidence supports a role for dysfunctional lipid processing and accumulation of harmful oxidized lipids in the pathogenesis of GA. Using oxidized low-density lipoprotein (ox-LDL)-induced RPE death assay, we screened and identified sterically-hindered phenol compounds with potent protective activities for RPE. Read More

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November 2021

GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis.

Mol Cell Endocrinol 2021 Nov 26:111519. Epub 2021 Nov 26.

Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address:

GATA-1 is a transcription factor from the GATA family, which features zinc fingers for DNA binding. This protein was initially identified as a crucial regulator of blood cell differentiation, but it is currently known that the Gata-1 gene expression is not limited to this system. Although the testis is also a site of significant GATA-1 expression, its role in testicular cells remains considerably unexplored. Read More

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November 2021

Analyses of polymorphisms of intron 2 of OPRK1 (kappa-opioid receptor gene) in association with opioid and cocaine dependence diagnoses in an African-American population.

Neurosci Lett 2021 Nov 26:136364. Epub 2021 Nov 26.

Laboratory of the Biology of Addictive Diseases, Laboratory of Statistical Genetics, the Rockefeller University, 1230 York Ave, New York, NY, 10065, USA.

Rationale The dynorphin/ kappa-opioid receptor (KOR) system (encoded by PDYN and OPRK1 genes respectively) is highly regulated by repeated exposure to drugs of abuse, including mu-opioid agonists and cocaine. These changes in the dynorphin/KOR system can then influence the rewarding effects of these drugs of abuse. Activation of the dynorphin/KOR system is also thought to have a role in the pro-addictive effects of stress. Read More

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November 2021

Identification of a novel catalytic inhibitor of topoisomerase II alpha that engages distinct mechanisms in p53 or p53 cells to trigger G2/M arrest and senescence.

Cancer Lett 2021 Nov 26. Epub 2021 Nov 26.

Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; NUS Centre for Cancer Research (N2CR), Yong Loo Lin School of Medicine, National University of Singapore, Singapore; NUS Medicine Healthy Longevity Program, National University of Singapore, Singapore; National University Cancer Institute, National University Health System, Singapore; Integrative Science and Engineering Programme (ISEP), NUS Graduate School (NUSGS), National University of Singapore, Singapore; Faculté de Medicine, University of Paris, Paris, France. Electronic address:

We report a novel topoisomerase IIα inhibitor, mercaptopyridine oxide (MPO), which induces G2/M arrest and senescence with distinctly different cell cycle regulators (p21 or p14) in HCT116p 53 and HCT116 p53 cells, respectively. MPO treatment induced defective topoisomerase IIα-mediated decatenation process and inhibition of the enzyme's catalytic activity that stalled M phase progression. Topoisomerase IIα inhibition was associated with ROS-mediated activation of ATM-Chk2 kinase axis in HCT116 p53 cells, but not in HCT116 p53 cells displaying early Chk1 activation. Read More

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November 2021

Silencing of cytochrome P450 gene CYP321A1 effects tannin detoxification and metabolism in Spodoptera litura.

Int J Biol Macromol 2021 Nov 26. Epub 2021 Nov 26.

State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address:

Cytochrome P450 monooxygenase (P450 or CYP) plays an important role in the metabolism of insecticides and plant allelochemicals by insects. CYP321B1, a novel Spodoptera litura P450 gene, was identified and characterized. CYP321B1 contains a 1488 bp open reading frame (ORF) that encodes a 495 amino acid protein. Read More

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November 2021

Role of the macula densa sodium glucose cotransporter type 1-nitric oxide synthase 1-tubuloglomerular feedback pathway in diabetic hyperfiltration.

Kidney Int 2021 Nov 26. Epub 2021 Nov 26.

Department of Molecular Pharmacology & Physiology, College of Medicine, University of South Florida, Tampa, FL.

An increase of glomerular filtration rate (GFR) is a common observation in early diabetes and is considered a key risk factor for subsequent kidney injury. However, the mechanisms underlying diabetic hyperfiltration have not been fully clarified. Here, we tested the hypothesis that macula densa neuronal nitric oxide synthase (NOS1) is upregulated via sodium glucose cotransporter type 1 (SGLT1) in diabetes, which then inhibits tubuloglomerular feedback (TGF) promoting glomerular hyperfiltration. Read More

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November 2021

MICE DISPLAY LEARNING AND BEHAVIORAL DEFICITS AFTER A 30-DAY SPACEFLIGHT ON BION-M1 SATELLITE.

Behav Brain Res 2021 Nov 26:113682. Epub 2021 Nov 26.

SSC RF Institute of biomedical problems 76A Khoroshevskoe av, Moscow, 123007, Russia.

Profound effects of spaceflight on the physiology of humans and non-human animals are well-documented but incompletely explored. Current goals to undertake interplanetary missions increase the urgency to learn more about adaptation to prolonged spaceflight and readaptation to Earth-normal conditions, especially with the inclusion of radiation exposures greater than those confronted in traditional, orbital flights. The 30-day-long Bion M-1 biosatellite flight was conducted at a relatively high orbit, exposing the mice to greater doses of radiation in addition to microgravity, a combination of factors relevant to Mars missions. Read More

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November 2021

Targeted Augmentation of Nuclear Gene Output (TANGO) of SCN1A Rescues Parvalbumin Interneuron Excitability and Reduces Seizures in a Mouse Model of Dravet Syndrome.

Brain Res 2021 Nov 26:147743. Epub 2021 Nov 26.

Department of Anesthesiology, University of Virginia Health System, Charlottesville, VA. Electronic address:

Dravet Syndrome (DS) is a severe developmental and epileptic encephalopathy typically caused by loss-of-function de novo mutations in the SCN1A gene which encodes the voltage-gated sodium channel isoform Na1.1. Decreased Na1. Read More

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November 2021

Loss of integrin α9β1 on tumor keratinocytes enhances the stromal vasculature and growth of cutaneous tumors.

J Invest Dermatol 2021 Nov 26. Epub 2021 Nov 26.

Department of Surgery; Department of Regenerative & Cancer Cell Biology, Albany Medical College, Albany, NY 12208, USA.

Angiogenesis is critical to tumor progression and the function of integrins in tumor angiogenesis is complex. Here we report that loss of integrin α9β1 expression from epidermal tumor cells is critical to maintain persistent stromal vessel density. Forced expression of α9 in transformed mouse keratinocytes dramatically reduces vessel density in allograft tumors, in vivo, compared to the same cells lacking α9β1. Read More

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November 2021

Aldo-ketoreductase 1c19 ablation does not affect insulin secretion in murine islets.

PLoS One 2021 29;16(11):e0260526. Epub 2021 Nov 29.

Department of Medicine and Naomi Berrie Diabetes Center, Vagelos College of Physicians & Surgeons of Columbia University, New York, New York, United States of America.

Beta cell failure is a critical feature of diabetes. It includes defects of insulin production, secretion, and altered numbers of hormone-producing cells. In previous work, we have shown that beta cell failure is mechanistically linked to loss of Foxo1 function. Read More

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November 2021

Gene expression signatures as candidate biomarkers of response to PD-1 blockade in non-small cell lung cancers.

PLoS One 2021 29;16(11):e0260500. Epub 2021 Nov 29.

Department of Pulmonary Medicine, Sendai Kousei Hospital, Sendai, Japan.

Although anti-PD-1/PD-L1 monotherapy has achieved clinical success in non-small cell lung cancer (NSCLC), definitive predictive biomarkers remain to be elucidated. In this study, we performed whole-transcriptome sequencing of pretreatment tumor tissue samples and pretreatment and on-treatment whole blood samples (WB) samples obtained from a clinically annotated cohort of NSCLC patients (n = 40) treated with nivolumab (anti-PD-1) monotherapy. Using a single-sample gene set enrichment scoring method, we found that the tumors of responders with lung adenocarcinoma (LUAD, n = 20) are inherently immunogenic to promote antitumor immunity, whereas those with lung squamous cell carcinoma (LUSC, n = 18) have a less immunosuppressive tumor microenvironment. Read More

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November 2021

Nucleo-cytoplasmic RNA distribution responsible for maintaining neuroinflammatory microenvironment.

RNA Biol 2021 Nov 29:1-15. Epub 2021 Nov 29.

Laboratory of Neurological Diseases and Brain Function, the Affiliated Hospital of Southwest Medical University, Luzhou, China.

Subcellular localization of transcripts is highly associated with regulation of gene expression, synthesis of protein, and also the development of the human brain cortex. Although many mechanisms are prevalent in the occurrence of neuroinflammation, the mechanisms based on differences in subcellular localization of transcripts have not been explored. To characterize the dynamic profile of nuclear and cytoplasmic transcripts during the progress of haemorrhage-induced neuroinflammation, we isolated nucleo-cytoplasmic RNA fractions of oxyhaemoglobin (oxy-Hb) treated microglia cells and sequenced both fractions. Read More

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November 2021

Oviduct epithelial cells-derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos.

Mol Reprod Dev 2021 Nov 29. Epub 2021 Nov 29.

Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.

Extracellular vesicles (EVs) carry bioactive cargoes involved in the early preimplantation development. This study investigated the effects of EVs obtained from an oviductal epithelial cell (OEC) conditioned medium on the developmental competence of in parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) porcine embryos. The OEC-EV-treated group showed significant increases in blastocyst formation and hatching rates compared to the control group (40. Read More

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November 2021

Biologia Futura: progress and future perspectives of long non-coding RNAs in forest trees.

Biol Futur 2021 Nov 29. Epub 2021 Nov 29.

Division of Plant Biotechnology and Cytogenetics, Institute of Forest Genetics and Tree Breeding, R.S. Puram, Coimbatore, 641002, India.

Forest trees are affected by climate change, anthropogenic pressure, as well as abiotic and biotic stresses. Conventional tree breeding has so far been limited to enhance overall productivity, and our understanding of the genetic basis of quantitative traits is still inadequate. Quantum leaps in next-generation sequencing technologies and bioinformatics have permitted the exploration and identification of various non-coding regions of the genome other than protein coding genes. Read More

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November 2021

Hsa-miR-199a-5p Protect Cell Injury in Hypoxia Induces Myocardial Cells Via Targeting HIF1α.

Mol Biotechnol 2021 Nov 29. Epub 2021 Nov 29.

Department of Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, People's Republic of China.

Myocardial infarction (MI) is one of the most common global diseases. Recently, microRNA 199a-5p (miR-199a-5p) has been recognized as a vital regulator in several human diseases. Nevertheless, the function of miR-199a-5p and the associated downstream molecular mechanisms in myocardial injury remain undescribed. Read More

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November 2021

A class I histone deacetylase HDA-2 is essential for embryonic development and size regulation of fertilized eggs in Caenorhabditis elegans.

Genes Genomics 2021 Nov 29. Epub 2021 Nov 29.

Department of Bioinformatics, College of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577, Japan.

Background: Caenorhabditis elegans encodes three class I histone deacetylases (HDACs), HDA-1, HDA-2, and HDA-3. Although HDA-1 is known to be involved in embryogenesis, the regulatory roles of HDA-2 and HDA-3 in embryonic development remain unexplored.

Objective: To elucidate the functional roles of the three class I HDACs in C. Read More

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November 2021

A Review on the Role of Endogenous Neurotrophins and Schwann Cells in Axonal Regeneration.

J Neuroimmune Pharmacol 2021 Nov 29. Epub 2021 Nov 29.

Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India, 576104.

Injury to the peripheral nerve is traditionally referred to acquired nerve injury as they are the result of physical trauma due to laceration, stretch, crush and compression of nerves. However, peripheral nerve injury may not be completely limited to acquired physical trauma. Peripheral nerve injury equally implies clinical conditions like Guillain-Barré syndrome (GBS), Carpal tunnel syndrome, rheumatoid arthritis and diabetes. Read More

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November 2021

Small RNAs Asserting Big Roles in Mycobacteria.

Noncoding RNA 2021 Oct 29;7(4). Epub 2021 Oct 29.

Departments of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9093, USA.

Tuberculosis (TB) is an infectious disease caused by (Mtb), with 10.4 million new cases per year reported in the human population. Recent studies on the Mtb transcriptome have revealed the abundance of noncoding RNAs expressed at various phases of mycobacteria growth, in culture, in infected mammalian cells, and in patients. Read More

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October 2021

Small but Mighty-The Emerging Role of snoRNAs in Hematological Malignancies.

Noncoding RNA 2021 Oct 26;7(4). Epub 2021 Oct 26.

Junior Research Group 'RBPs and ncRNAs in Human Diseases', Medical Faculty, Martin-Luther-University Halle-Wittenberg, 06120 Halle, Saale, Germany.

Over recent years, the long known class of small nucleolar RNAs (snoRNAs) have gained interest among the scientific community, especially in the clinical context. The main molecular role of this interesting family of non-coding RNAs is to serve as scaffolding RNAs to mediate site-specific RNA modification of ribosomal RNAs (rRNAs) and small nuclear RNAs (snRNAs). With the development of new sequencing techniques and sophisticated analysis pipelines, new members of the snoRNA family were identified and global expression patterns in disease backgrounds could be determined. Read More

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October 2021

Lysosomal Function Impacts the Skeletal Muscle Extracellular Matrix.

J Dev Biol 2021 Nov 23;9(4). Epub 2021 Nov 23.

School of Biology and Ecology, University of Maine, Orono, ME 04469, USA.

Muscle development and homeostasis are critical for normal muscle function. A key aspect of muscle physiology during development, growth, and homeostasis is modulation of protein turnover, the balance between synthesis and degradation of muscle proteins. Protein degradation depends upon lysosomal pH, generated and maintained by proton pumps. Read More

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November 2021

MYCN in Neuroblastoma: "Old Wine into New Wineskins".

Diseases 2021 Oct 29;9(4). Epub 2021 Oct 29.

Choremeio Research Laboratory, First Department of Pediatrics, National and Kapodistrian University of Athens, Thivon & Levadeias 8, Goudi, 11527 Athens, Greece.

MYCN Proto-Oncogene, BHLH Transcription Factor (MYCN) has been one of the most studied genes in neuroblastoma. It is known for its oncogenetic mechanisms, as well as its role in the prognosis of the disease and it is considered one of the prominent targets for neuroblastoma therapy. In the present work, we attempted to review the literature, on the relation between MYCN and neuroblastoma from all possible mechanistic sites. Read More

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October 2021