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HSP17.4 mediates salicylic acid and jasmonic acid pathways in the regulation of resistance to Colletotrichum gloeosporioides in strawberry.

Mol Plant Pathol 2021 May 5. Epub 2021 May 5.

Institute of Forestry and Pomology, Shanghai Academy of Agricultural Sciences, Shanghai, China.

In this study, we used virus-mediated gene silencing technology and found that the HSP17.4 gene-silenced cultivar Sweet Charlie plants were more susceptible to Colletotrichum gloeosporioides than the wild-type Sweet Charlie, and the level of infection was even higher than that of the susceptible cultivar Benihopp. The results of differential quantitative proteomics showed that after infection with the pathogen, the expression of the downstream response genes NPR1, TGA, and PR-1 of the salicylic acid (SA) signalling pathway was fully up-regulated in the wild-type Sweet Charlie, and the expression of the core transcription factor MYC2 of the jasmonic acid (JA) pathway was significantly down-regulated. Read More

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Molecular Characterization of Three B-1,4-Endoglucanase Genes in and Functional Analysis of -2 Gene.

Plants (Basel) 2021 Mar 17;10(3). Epub 2021 Mar 17.

Istituto per la Protezione Sostenibile delle Piante (IPSP), Consiglio Nazionale delle Ricerche (CNR), S.S. Bari, Via G. Amendola 122/D, 70126 Bari, Italy.

is an important root-lesion nematode that causes damage to tea plantations in Iran and all over the world. The present study reports on the characterization and evolution of three ß-1,4-endoglucanase genes: -2, -3 and -4. The gene structure of -2 was fully determined with the predicted signal peptide and devoid of the linker domain and carbohydrate-binding domain, while -3 and -4 were only partially sequenced. Read More

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Exosomes derived from mesenchymal stem cells regulate Treg/Th17 balance in aplastic anemia by transferring miR-23a-3p.

Clin Exp Med 2021 Mar 29. Epub 2021 Mar 29.

Department of Pediatrics, Renmin Hospital of Wuhan University, No. 99 Zhangzhidong Road, Wuhan, 430060, China.

Imbalanced Th17/Treg ratio is implicated in the pathogenesis of aplastic anemia. Studies have indicated that bone marrow-derived mesenchymal stem cells-derived exosomes (BMSC-Exo) could correct imbalanced Th17/Treg in aplastic anemia, but the mechanism remains not fully understand. This study was designed to investigate whether BMSC-Exo regulates the Th17/Treg balance in aplastic anemia by transferring miR-23a-3p. Read More

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Molecular Characterization and Functional Analysis of the 90-1 Gene in Relation to Temperature Changes in .

Front Physiol 2021 23;12:615653. Epub 2021 Feb 23.

Institute for Sustainable Plant Protection-CNR, Bari, Italy.

Understanding how entomopathogenic nematodes respond to temperature changes and have adapted to the local environment is crucial to improve their potential as biocontrol agents. In order to improve understanding of 's potential adaptability to future climate changes, full-length cDNA and the corresponding gene of heat shock protein 90 (Hsp90) were isolated and fully characterized. The reproductive potential of the Apulian strain of increased when the temperature rose from 23 to 30°C, but no reproduction was found at 12°C. Read More

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February 2021

An interaction between PRRT2 and Na/K ATPase contributes to the control of neuronal excitability.

Cell Death Dis 2021 Mar 17;12(4):292. Epub 2021 Mar 17.

Department of Experimental Medicine, University of Genova, Viale Benedetto XV, 3, 16132, Genoa, Italy.

Mutations in PRoline Rich Transmembrane protein 2 (PRRT2) cause pleiotropic syndromes including benign infantile epilepsy, paroxysmal kinesigenic dyskinesia, episodic ataxia, that share the paroxysmal character of the clinical manifestations. PRRT2 is a neuronal protein that plays multiple roles in the regulation of neuronal development, excitability, and neurotransmitter release. To better understand the physiopathology of these clinical phenotypes, we investigated PRRT2 interactome in mouse brain by a pulldown-based proteomic approach and identified α1 and α3 Na/K ATPase (NKA) pumps as major PRRT2-binding proteins. Read More

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A novel allele of SIR2 reveals a heritable intermediate state of gene silencing.

Genetics 2021 Mar 13. Epub 2021 Mar 13.

Department of Molecular and Cell Biology; University of California, Berkeley; Berkeley, CA.

Genetic information acquires additional meaning through epigenetic regulation, the process by which genetically identical cells can exhibit heritable differences in gene expression and phenotype. Inheritance of epigenetic information is a critical step in maintaining cellular identity and organismal health. In Saccharomyces cerevisiae, one form of epigenetic regulation is the transcriptional silencing of two mating-type loci, HML and HMR, by the SIR-protein complex. Read More

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Rap2B promotes the proliferation and migration of human glioma cells via activation of the ERK pathway.

Oncol Lett 2021 Apr 23;21(4):314. Epub 2021 Feb 23.

Department of Ultrasound, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.

Glioma is one of the most common primary brain tumors and has a poor prognosis. Rap2B, a member of the Ras family of oncogenes, is highly expressed and promotes the progression of several tumors, including glioma. However, the mechanism underlying the role of Rap2B in glioma is not fully understood. Read More

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CTCF-silenced miR-137 contributes to EMT and radioresistance in esophageal squamous cell carcinoma.

Cancer Cell Int 2021 Mar 8;21(1):155. Epub 2021 Mar 8.

Department of Radiation Oncology, Xuzhou Cancer Hospital, No. 131, Huancheng Road, Gulou District, Xuzhou, 221005, Jiangsu, China.

Background: Esophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors in gastrointestinal system. MicroRNAs (miRNAs) have been reported to be implicated in cancer development. However, the role of miR-137 has not been fully revealed in ESCC. Read More

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LINC01224 accelerates malignant transformation via MiR-193a-5p/CDK8 axis in gastric cancer.

Cancer Med 2021 02;10(4):1377-1393

Oncology Department, Jiangdu People's Hospital Affiliated to Medical College of Yangzhou University, Yangzhou, Jiangsu, China.

Background: Gastric cancer (GC) is a malignant tumor with a significantly high mortality rate, yet, its pathogenesis is not fully understood. Bioinformatics predicted that LINC01224 is highly expressed in stomach adenocarcinoma (STAD), and showed that LINC01224 adsorbed miR-193a-5p to target CDK8. Therefore, this study intended to verify the effect of the LINC01224/miR-193a-5p/CDK8 axis on the biological behavior of gastric cancer. Read More

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February 2021

Development of a novel, fully human, anti-PCSK9 antibody with potent hypolipidemic activity by utilizing phage display-based strategy.

EBioMedicine 2021 Mar 26;65:103250. Epub 2021 Feb 26.

Department of Cell and Molecular Biology, School of Life Science and Technology, State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, China Pharmaceutical University, Nanjing 210009, P.R. China. Electronic address:

Background: Proprotein convertase subtilisin/kexin type 9 (PCSK9) regulates serum LDL cholesterol (LDL-C) levels by facilitating the degradation of the LDL receptor (LDLR) and is an attractive therapeutic target for hypercholesterolemia intervention. Herein, we generated a novel fully human antibody with favourable druggability by utilizing phage display-based strategy.

Methods: A potent single-chain variable fragment (scFv) named AP2M21 was obtained by screening a fully human scFv phage display library with hPCSK9, and performing two in vitro affinity maturation processes including CDR-targeted tailored mutagenesis and cross-cloning. Read More

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Identification of Tick Midgut Genes Differentially Expressed During the Transmission of Spirochetes Using a Transcriptomic Approach.

Front Immunol 2020 4;11:612412. Epub 2021 Feb 4.

Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Ceske Budejovice, Czechia.

Lyme borreliosis is an emerging tick-borne disease caused by spirochetes sensu lato. In Europe, Lyme borreliosis is predominantly caused by and transmitted by . Although behavior throughout tick development is quite well documented, specific molecular interactions between and the tick have not been satisfactorily examined. Read More

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February 2021

COR-101, ein menschlicher Antikörper gegen COVID-19.

Biospektrum (Heidelb) 2021 12;27(1):46-48. Epub 2021 Feb 12.

Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstraße 7, D-38106 Braunschweig, Deutschland.

COR-101 is a fully human, Fc silenced IgG that was discovered by antibody phage display. It reduced the SARS-CoV-2 virus load in the lung by more than 99 percent in Hamster models and led to much faster recovery. Its mode of action has been elucidated by solving the atomic structure of its interaction with SARS-CoV-2. Read More

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February 2021

CircPPP1R12A promotes the progression of colon cancer through regulating CTNNB1 via sponging miR-375.

Anticancer Drugs 2021 06;32(6):635-646

Department of Gastroenterology, First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Circular RNAs (circRNAs) have been identified as potential biomarkers for many cancer, including colon cancer (CC). However, the function and mechanism of circPPP1R12A in CC have not been fully elucidated. Quantitative real-time PCR was employed to assess the expression of circPPP1R12A, microRNA (miR)-375 and catenin beta-1 (CTNNB1). Read More

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A risk progression breast epithelial 3D culture model reveals Cx43/hsa_circ_0077755/miR-182 as a biomarker axis for heightened risk of breast cancer initiation.

Sci Rep 2021 Jan 29;11(1):2626. Epub 2021 Jan 29.

Department of Biology, Faculty of Arts and Sciences, American University of Beirut, P.O. Box: 11-0236, Beirut, Lebanon.

mRNA-circRNA-miRNAs axes have been characterized in breast cancer, but not as risk-assessment axes for tumor initiation in early-onset breast cancer that is increasing drastically worldwide. To address this gap, we performed circular RNA (circRNA) microarrays and microRNA (miRNA) sequencing on acini of HMT-3522 S1 (S1) breast epithelial risk-progression culture model in 3D and chose an early-stage population miRNome for a validation cohort. Nontumorigenic S1 cells form fully polarized epithelium while pretumorigenic counterparts silenced for gap junction Cx43 (Cx43-KO-S1) lose epithelial polarity, multilayer and mimic premalignant in vivo mammary epithelial morphology. Read More

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January 2021

An insight into the functional role of antioxidant and detoxification enzymes in adult Rhipicephalus microplus female ticks.

Parasitol Int 2021 Apr 19;81:102274. Epub 2020 Dec 19.

Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500 Porto Alegre, RS, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Brazil; Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9090 Porto Alegre, RS, Brazil. Electronic address:

Ticks have developed physiological adaptations to transport, store, metabolize and secrete toxic components from the diet and environment. Different classes of enzymes are involved in these processes, however, the role of several of them is not yet characterized in Rhipicephalus microplus. In this context, this work investigated the action of antioxidant and detoxification enzymes, as well as the levels of essential cellular reductants in R. Read More

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Epigenetic Silencing of Tumor Suppressor miR-124 Directly Supports STAT3 Activation in Cutaneous T-Cell Lymphoma.

Cells 2020 12 15;9(12). Epub 2020 Dec 15.

Department of Dermatology, Institut Hospital del Mar d'Investigacions Mèdiques (IMIM), Universitat Autònoma Barcelona, Carrer del Dr. Aiguader, 88, 08003 Barcelona, Spain.

Increasing evidence supports a potential role for STAT3 as a tumor driver in cutaneous T-cell lymphomas (CTCL). The mechanisms leading to STAT3 activation are not fully understood; however, we recently found that miR-124, a known STAT3 regulator, is robustly silenced in MF tumor-stage and CTCL cells.

Objective: We studied here whether deregulation of miR-124 contributes to STAT3 pathway activation in CTCL. Read More

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December 2020

Proteomic Analysis of Potato Responding to the Invasion of UW551 and Its Type III Secretion System Mutant.

Mol Plant Microbe Interact 2021 Apr 24;34(4):337-350. Epub 2021 Mar 24.

Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.

The infection of potato with UW551 gives rise to bacterial wilt disease via colonization of roots. The type III secretion system (T3SS) is a determinant factor for the pathogenicity of . To fully understand perturbations in potato by type III effectors(T3Es), we used proteomics to measure differences in potato root protein abundance after inoculation with UW551 and the T3SS mutant (UW551△V). Read More

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lncRNA SNHG14 involved in trophoblast cell proliferation, migration, invasion and epithelial-mesenchymal transition by targeting miR-330-5p in preeclampsia.

Zygote 2021 Apr 9;29(2):108-117. Epub 2020 Nov 9.

Department of Obstetrics, Huai'an First People's Hospital/The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, Huai'an, 223300, Jiangsu, China.

Preeclampsia (PE), a pregnancy-specific disease, has become one of the leading causes of maternal and neonatal morbidity and mortality. Pathogenesis of PE has still not been fully addressed and there is a great need to develop early diagnosis markers and effective therapy. This study aimed to determine if lncRNA SNHG14 has a protective effect on placental trophoblast and prevents PE. Read More

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COL11A1 Was Involved in Cell Proliferation, Apoptosis and Migration in Non-Small Cell Lung Cancer Cells.

J Invest Surg 2020 Nov 5:1-6. Epub 2020 Nov 5.

Department of Integrated Traditional Chinese and Western Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Background: Type XI collagen (COL11A1) was reported to associate with malignancy in several cancer types, whereas its role in lung cancer is not fully understood. Therefore, the present study aimed to explore the expression level and biological role of COL11A1 in lung cancer cells.

Methods: Gene Expression Omnibus (GEO) database containing 6 lung cancer tissues and normal lung tissues was used to identify potential aberrantly expressed genes. Read More

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November 2020

20(S)-Protopanaxadiol inhibits epithelial-mesenchymal transition by promoting retinoid X receptor alpha in human colorectal carcinoma cells.

J Cell Mol Med 2020 12 30;24(24):14349-14365. Epub 2020 Oct 30.

Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, Changchun, China.

Colorectal carcinoma (CRC) recurrence is often accompanied by metastasis. Most metastasis undergo through epithelial-mesenchymal transition (EMT). Studies showed that retinol X receptor alpha (RXRα) and 20(S)-Protopanaxadiol (PPD) have anti-tumour effects. Read More

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December 2020

Inhibition of MUC1 exerts cell-cycle arrest and telomerase suppression in glioblastoma cells.

Sci Rep 2020 10 26;10(1):18238. Epub 2020 Oct 26.

Department of Neurosurgery, Seoul National University College of Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Korea.

Mucin 1 (MUC1) is a transmembrane glycoprotein involved in tumorigenesis of diverse cancers. However, the role of MUC1 in glioblastoma (GBM) has not yet been fully explored. In this study, the anticancer mechanism of MUC1 suppression in GBM was investigated. Read More

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October 2020

Restoring the epigenetically silenced PCK2 suppresses renal cell carcinoma progression and increases sensitivity to sunitinib by promoting endoplasmic reticulum stress.

Theranostics 2020 15;10(25):11444-11461. Epub 2020 Sep 15.

Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Tumors have significant abnormalities in various biological properties. In renal cell carcinoma (RCC), metabolic abnormalities are characteristic biological dysfunction that cannot be ignored. Despite this, many aspects of this dysfunction have not been fully explained. Read More

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September 2020

Regulation of High-Altitude Hypoxia on the Transcription of CYP450 and UGT1A1 Mediated by PXR and CAR.

Front Pharmacol 2020 17;11:574176. Epub 2020 Sep 17.

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.

Little is known about what roles the pregnane X receptor (PXR) and constitutive androstane receptor (CAR) play in drug metabolism in high-altitude hypoxia. Likewise, the potential interaction of nuclear receptors and drug metabolism enzymes during drug metabolism of high-altitude hypoxia is not fully understood. In this work, we investigated the effects of high-altitude hypoxia on transcriptional regulation of cytochrome P450 (CYP450) and UDP-glucuronosyltransferase 1A1 (UGT1A1) genes mediated by PXR and CAR proteins. Read More

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September 2020

MiR-381-3p/RAB2A axis activates cell proliferation and inhibits cell apoptosis in bladder cancer.

Cell Mol Biol (Noisy-le-grand) 2020 Sep 30;66(6):117-120. Epub 2020 Sep 30.

Department of Urology, Qilu Hospital of Shandong University , Jinan, 250012, China.

Acting as a really common cancer in the world, bladder cancer has taken many people's life away. MiRNAs and mRNA have been reported can regulate the expression of cancers. In this study, the role of RAB2A and miR-381-3p was fully studied in bladder cancer. Read More

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September 2020

Unique and contrasting effects of light and temperature cues on plant transcriptional programs.

Transcription 2020 Jun - Aug;11(3-4):134-159. Epub 2020 Oct 4.

Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB , Barcelona, Spain.

Plants have adapted to tolerate and survive constantly changing environmental conditions by reprogramming gene expression in response to stress or to drive developmental transitions. Among the many signals that plants perceive, light and temperature are of particular interest due to their intensely fluctuating nature which is combined with a long-term seasonal trend. Whereas specific receptors are key in the light-sensing mechanism, the identity of plant thermosensors for high and low temperatures remains far from fully addressed. Read More

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October 2020

TLR4/AP-1-Targeted Anti-Inflammatory Intervention Attenuates Insulin Sensitivity and Liver Steatosis.

Mediators Inflamm 2020 16;2020:2960517. Epub 2020 Sep 16.

Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Insulin resistance has been shown to be the common pathogenesis of many metabolic diseases. Metainflammation is one of the important characteristics of insulin resistance. Macrophage polarization mediates the production and development of metainflammation. Read More

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September 2020

Fatty Acid Synthase Beta Dehydratase in the Lipid Biosynthesis Pathway Is Required for Conidiogenesis, Pigmentation and Appressorium Formation in S6.

Int J Mol Sci 2020 Sep 30;21(19). Epub 2020 Sep 30.

Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia; UKM Bangi 43600, Malaysia.

Lipid biosynthesis produces glycerol, which is important in fueling turgor pressure necessary for germination and penetration of plant host by fungi. As the relationship between pathogenicity and the lipid biosynthetic pathway is not fully understood, we have elucidated the role of the fatty acid synthase beta subunit dehydratase () gene in lipid biosynthesis. The gene was silenced through homologous double crossover in strain S6 to study the effect on lipid biosynthesis. Read More

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September 2020

Knockdown of CRAD suppresses the growth and promotes the apoptosis of human lung cancer cells via Claudin 4.

Biosci Rep 2020 10;40(10)

College of Basic Medicine, Jining Medical University, Jining, Shandong 272067, P.R. China.

Non-small cell lung cancer (NSCLC) is one of the most common causes of cancer-related mortality globally. However, the mechanism underlying NSCLC is not fully understood. Here, we investigated the role of cancer-related regulator of actin dynamics (CRAD) in NSCLC. Read More

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October 2020

DNA demethylase ROS1 prevents inheritable DREB1A/CBF3 repression by transgene-induced promoter methylation in the Arabidopsis ice1-1 mutant.

Plant Mol Biol 2020 Dec 30;104(6):575-582. Epub 2020 Sep 30.

Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan.

Key Message: In the ros1-defective mutant, DREB1A repression by the transgene-induced promoter methylation of ice1-1 became inheritable across generations even in the absence of the causative transgene NICE1. Transgene silencing (TGS) is a widely observed event during plant bioengineering, which is presented as a gradual decrease in ectopic gene expression across generations and occasionally coupled with endogenous gene silencing based on DNA sequence similarity. TGS is known to be established by guided DNA methylation machinery. Read More

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December 2020

Long-isoform NRF1 protects against arsenic cytotoxicity in mouse bone marrow-derived mesenchymal stem cells by suppressing mitochondrial ROS and facilitating arsenic efflux.

Toxicol Appl Pharmacol 2020 11 25;407:115251. Epub 2020 Sep 25.

Laboratory of Chronic Diseases and Environmental Genetics, School of Public Health, China Medical University, Shenyang, Liaoning 110122, PR China; Experimental Teaching Center, School of Public Health, China Medical University, Shenyang, Liaoning 110122, PR China; The Key Laboratory of Liaoning Province on Toxic and Biological Effects of Arsenic, School of Public Health, China Medical University, Shenyang, Liaoning 110122, PR China. Electronic address:

Acute exposure to arsenic is known to cause bone marrow depression and result in anemia, in which the dusfunction of cells in the bone marrow niche such as mesenchymal stem cells (MSCs) is vital. However, the mechanism underlying response of MSCs to arsenic challange is not fully understood. In the present study, we investigated the role of nuclear factor erythroid 2-related factor (NRF) 1 (NRF1), a sister member of the well-known master regulator in antioxidative response NRF2, in arsenite-induced cytotoxicity in mouse bone marrow-derived MSCs (mBM-MSCs). Read More

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November 2020