1,161 results match your criteria fsh-beta mrna

Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene, Nr4a3, and its suppressor Anxa5 in the pituitary gland of female rats.

J Reprod Dev 2021 Apr 10. Epub 2021 Apr 10.

Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan.

Functional relationship between nuclear receptor subfamily 4 group A member 3 (Nr4a3) and annexin A5 (Anxa5), which are two gonadotropin-releasing hormone (GnRH)-inducible genes, has been established while evaluating pituitary gonadotropes in relation to follicle-stimulating hormone beta (Fshb) expression. However, the physiological variations that arise due to the differential expression of these genes in the pituitary gland during rat estrous cycle remain unknown. This study aimed to evaluate the Nr4a3 and Anxa5 mRNA expression during the estrous cycle in rats in comparison with the expression of the gonadotropin subunit genes, luteinizing hormone beta (Lhb) and Fshb. Read More

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Molecular characterization and expression analysis of anti-Müllerian hormone in common carp (Cyprinus carpio) adult testes.

Gene Expr Patterns 2021 Mar 3;40:119169. Epub 2021 Mar 3.

Reproductive and Molecular Biology Group, Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil. Electronic address:

Anti-Müllerian hormone (Amh) is a member of the transforming growth factor-β (Tgf-β) superfamily required in the regression of Müllerian ducts during gonadal sex differentiation of higher vertebrates. Teleost fish lack Müllerian ducts, but identified Amh orthologs have been shown to exert crucial functions during sex determination and differentiation of several species of teleosts. However, the function of Amh during gametogenesis in adult fish remains poorly investigated. Read More

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TGFBR3L-An Uncharacterised Pituitary Specific Membrane Protein Detected in the Gonadotroph Cells in Non-Neoplastic and Tumour Tissue.

Cancers (Basel) 2020 Dec 31;13(1). Epub 2020 Dec 31.

Department of Immunology, Genetics and Pathology, Uppsala University, Dag Hammarskjöldsväg 20, 752 37 Uppsala, Sweden.

Here, we report the investigation of transforming growth factor beta-receptor 3 like (TGFBR3L), an uncharacterised pituitary specific membrane protein, in non-neoplastic anterior pituitary gland and pituitary neuroendocrine tumours. A polyclonal antibody produced within the Human Protein Atlas project (HPA074356) was used for TGFBR3L staining and combined with SF1 and FSH for a 3-plex fluorescent protocol, providing more details about the cell lineage specificity of TGFBR3L expression. A cohort of 230 pituitary neuroendocrine tumours were analysed. Read More

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December 2020

In vitro differentiation of theca cells from ovarian cells isolated from postmenopausal women.

Hum Reprod 2020 Dec;35(12):2793-2807

Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.

Study Question: Can human theca cells (TCs) be differentiated in vitro?

Summary Answer: It is possible to differentiate human TCs in vitro using a medium supplemented with growth factors and hormones.

What Is Known Already: There are very few studies on the origin of TCs in mammalian ovaries. Precursor TCs have been described in neonatal mice ovaries, which can differentiate into TCs under the influence of factors from oocytes and granulosa cells (GCs). Read More

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December 2020

In vitro long-term culture of isolated ovine preantral follicles: Influence of ethanol on steroid production, oocyte meiotic resumption, and metabolomic profile.

Res Vet Sci 2021 Mar 2;135:432-441. Epub 2020 Nov 2.

Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceara, Itaperi Campus, 60740-903 Fortaleza, CE, Brazil. Electronic address:

Ethanol is used routinely to dilute cell culture media supplements with little or no water solubility. This study evaluates the effect of low concentration of ethanol on the follicular development, oocyte maturation, hormone production, gene expression, and metabolomics profile of spent culture medium after long-term culture of isolated ovine preantral follicles. For this, follicles were cultured for 18 days in α-Minimum Essential Medium alone (control treatment) or supplemented with 100 ng/mL recombinant bovine FSH (rbFSH treatment) or with 0. Read More

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Changes in nitric oxide, carbon monoxide, hydrogen sulfide and male reproductive hormones in response to chronic restraint stress in rats.

Amira Moustafa

Free Radic Biol Med 2021 Jan 29;162:353-366. Epub 2020 Oct 29.

Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig, Egypt. Electronic address:

Gasotrasmitters are endogenously synthesized gaseous molecules that are engaged in cellular physiological and pathological processes. Stress influences various physiological aspects of an organism and amends a normal system's functions, including those of the reproductive system. This study aims to investigate the effect of long-term exposure to restraint stress on the male reproductive system as well as the possible impact of stress on the levels of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (HS), and the expression of their producing-enzymes. Read More

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January 2021

Effects of Kiss2 on the Expression of Gonadotropin Genes in the Pituitary of Nile Tilapia ().

Dev Reprod 2020 Sep 30;24(3):149-158. Epub 2020 Sep 30.

Dept. of Aquatic Life Medical Sciences, Sunmoon University, Asan 31460, Korea.

Kisspeptin, expressed mainly in the hypothalamus, stimulates gonadotropin-releasing hormone neurons to facilitate reproduction. In some model animals, the kisspeptin is also expressed in the pituitary. Recently, a pathway has been suggested in which kisspeptin acts directly on the pituitary to secretion of gonadotropin in mammals. Read More

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September 2020

Effects of allopregnanolone on central reproductive functions in sheep under natural and stressful conditions.

Theriogenology 2020 Dec 11;158:138-147. Epub 2020 Sep 11.

Department of Animal Physiology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3 St, 05-110, Jablonna, Poland. Electronic address:

Reproductive functions may be affected by internal and external factors that are integrated in the central nervous system (CNS). Stressful stimuli induce the neuroendocrine response of the hypothalamic-pituitary-adrenal axis, as well as the synthesis of the neurosteroid allopregnanolone (AL) in the brain. This study tested the hypothesis that centrally administered AL could affect the expression of certain genes involved in reproductive functions at the hypothalamus and pituitary levels, as well as pulsatile gonadotropin secretion in sheep under both natural and stressful conditions. Read More

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December 2020

The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature.

Poult Sci 2020 Sep 20;99(9):4586-4597. Epub 2020 Jun 20.

Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan. Electronic address:

Reproductive failure associated with heat stress is a well-known phenomenon in poultry. High temperatures also induce various metabolic disturbances in many animals. Because the liver plays a central role in metabolism, the present study aimed to clarify the relationship between liver and reproduction in Japanese quails exposed to high temperatures. Read More

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September 2020

The content of gonadotropin-releasing hormone (GnRH), kisspeptin, and estrogen receptors (ERα/ERβ) in the anteromedial hypothalamus displays daily variations throughout the rat estrous cycle.

Cell Tissue Res 2020 Sep 24;381(3):451-460. Epub 2020 Jul 24.

Neuroendocrinology Laboratory, Reproductive Biology Research Unit, Faculty of High Studies Zaragoza, National Autonomous University of Mexico, 09230, Mexico City, Mexico.

The content of gonadotropin-releasing hormone (GnRH), its mRNA, and estrogen receptor alpha (ERα) and beta (ERβ) in the hypothalamus varies throughout the estrous cycle. Furthermore, the abundance of these molecules displays asymmetry between the right and left side. In the present study, we investigated the changes in the content of ERα, ERβ, kisspeptin, and GnRH by western blot in the left and right anteromedial hypothalamus, at four different times during each stage of the rat estrous cycle. Read More

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September 2020

Differential distribution and potential regulatory roles of estrogen receptor 2a and 2b in the pituitary of ricefield eel Monopterus albus.

Gen Comp Endocrinol 2020 11 18;298:113554. Epub 2020 Jul 18.

Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China; Biology Department, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China. Electronic address:

Estrogens play important regulatory roles in the pituitary of vertebrates. Two forms of estrogen receptor 2 (Esr2), namely Esr2a and Esr2b, are identified in teleosts, but their differential roles remain to be fully elucidated. In the present study, expression and potential functional roles of Esr2a and Esr2b were characterized in ricefield eels. Read More

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November 2020

"" Effect of Different Follicle-Stimulating Hormone Preparations on Sertoli Cells: Toward a Personalized Treatment for Male Infertility.

Front Endocrinol (Lausanne) 2020 18;11:401. Epub 2020 Jun 18.

Research Unit on Human Reproduction, International Scientific Institute Paul VI, Rome, Italy.

Follicle-stimulating hormone (FSH), a major regulator of spermatogenesis, has a crucial function in the development and function of the testis and it is extensively given as a fertility treatment to stimulate spermatogenesis. We analyzed the effects of different FSH preparations (α-follitropin, β-follitropin, and urofollitropin) in combination with testosterone on porcine pre-pubertal Sertoli cells. To study the effect of the different FSH treatments in the Sertoli cell function we performed Real Time PCR analysis of AMH, inhibin B, and FSH-r, an ELISA assay for AMH and inhibin B, and a high-throughput comparative proteomic analysis. Read More

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Bisphenol A in utero exposure induces ovary dysfunction in mice offspring and the ameliorating effects of Cuscuta chinensis flavonoids.

Environ Sci Pollut Res Int 2020 Sep 2;27(25):31357-31368. Epub 2020 Jun 2.

Institute of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding, 071001, China.

To study the alleviating effects of flavonoids from Cuscuta chinensis (CCFs) on ovary injury in female offspring of pregnant mice exposed to BPA, five groups (n = 20) of pregnant mice were intragastrically administrated with BPA (5 mg/kg/day) and CCFs (20 mg/kg/day, 30 mg/kg/day, 40 mg/kg/day) at pregnancy days 1-18. The ovaries and serum of F1 female mice were collected at postnatal day (PND) 21 and PND 56 for the detection of related indicators. The ovarian and testicular histomorphologies were observed with hematoxylin-eosin staining (H&E). Read More

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September 2020

Clinical and genetic analysis of an isolated follicle-stimulating hormone deficiency female patient.

J Assist Reprod Genet 2020 Jun 5;37(6):1441-1448. Epub 2020 May 5.

Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology, 1095 JieFang Avenue, Wuhan, 430030, People's Republic of China.

Objective: To characterize the clinical features of a female patient with isolated follicle-stimulating hormone (FSH) deficiency and to investigate the underlying mechanisms of FSH inactivation.

Methods: The proband was a 29-year-old woman with primary amenorrhea, impaired pubertal development, and infertility. Subsequently, reproductive endocrine was screened. Read More

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Seasonal expressions of SPAG11A and androgen receptor in the epididymis of the wild ground squirrels (Citellus dauricus Brandt).

Eur J Histochem 2020 Apr 21;64(2). Epub 2020 Apr 21.

Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing.

Sperm-associated antigen 11A (SPAG11A) is expressed exclusively in the epididymis, which can specifically regulate sperm maturation. The aim of this study was to investigate the seasonal expressions of beta-defensin (SPAG11A) and androgen receptor (AR) in the epididymis of the wild ground squirrels (Citellus dauricus Brandt). Morphologically, the results showed that epididymis length and weight in the breeding season were significantly higher than those of the non-breeding season. Read More

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Discovery of new receptors regulating luteinizing hormone and follicle-stimulating hormone secretion by bovine gonadotrophs to explore a new paradigm for mechanisms regulating reproduction.

Hiroya Kadokawa

J Reprod Dev 2020 Aug 6;66(4):291-297. Epub 2020 Apr 6.

Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

Previous studies in the 1960s and 1970s have reported that both gonadotropin-releasing hormone (GnRH) and estradiol-activated nuclear estrogen receptors regulate gonadotropin secretion in women. However, I had previously reported that gonadotroph function is regulated by complex crosstalk between several membrane receptors. RNA-seq had previously revealed 259 different receptor genes expressed in the anterior pituitary of heifers. Read More

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Post-photostimulation energy intake accelerated pubertal development in broiler breeder pullets.

Poult Sci 2020 Apr 28;99(4):2215-2229. Epub 2020 Feb 28.

Department of Agricultural, Food and Nutritional Science, 410 Agriculture/Forestry Centre, University of Alberta, Edmonton, AB, Canada, T6G 2P5. Electronic address:

The effect of ME intake (MEI) on the reproductive system was evaluated. Ross 308 broiler breeder pullets (n = 140) were assigned to 2 treatments from 22 to 26 wk of age: (1) Low-energy diet fed restricted (2,807 kcal/kg, low MEI) and (2) high-energy diet fed unrestricted (3,109 kcal/kg, high MEI). Daylength was increased from 8 to 14 h at 22 wk of age with a light intensity of 30 lux. Read More

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Human Follicle-Stimulating Hormone ß Subunit Expression Depends on FOXL2 and SMAD4.

Endocrinology 2020 05;161(5)

Department of Pharmacology and Therapeutics, McGill University, Montréal, Québec, Canada.

Follicle-stimulating hormone (FSH), an essential regulator of mammalian fertility, is synthesized by pituitary gonadotrope cells in response to activins. In mice, activins signal via SMAD3, SMAD4, and FOXL2 to regulate transcription of the FSHβ subunit (Fshb) gene. Gonadotrope-specific deletion of Foxl2, alone or in combination with Smad4, renders mice FSH-deficient. Read More

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HIF1 driven transcriptional activity regulates steroidogenesis and proliferation of bovine granulosa cells.

Sci Rep 2020 03 3;10(1):3906. Epub 2020 Mar 3.

Experimental Reproductive Biology Unit, Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), 18196, Dummerstorf, Germany.

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor, consisting of a constitutively expressed β-subunit (HIF1B) and a regulated α-subunit (HIF1A). In the present study, we analyzed the HIF1 driven transcriptional activity in bovine granulosa cells (GC). Treatment of GC with FSH (follicle stimulating hormone) and IGF1 (insulin-like growth factor 1) resulted in the upregulation of HIF1A mRNA expression under normoxia. Read More

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Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids.

J Reprod Dev 2020 Jun 28;66(3):249-254. Epub 2020 Feb 28.

Laboratory of Environmental Physiology, Department of Life Science, School of Science and Engineering, Kindai University, Higashiosaka 577-8502, Japan.

GPR120 is a long-chain fatty acid (LCFA) receptor that is specifically expressed in gonadotropes in the anterior pituitary gland in mice. The aim of this study was to investigate whether GPR120 is activated by free fatty acids in the pituitary of mice and mouse immortalized gonadotrope LβT2 cells. First, the effects of palmitate on GPR120, gonadotropic hormone b-subunits, and GnRH-receptor expression in gonadotropes were investigated in vitro. Read More

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Effect of cassic acid on immunity and immune-reproductive genes transcription in Clarias gariepinus against Edwardsiella tarda.

Fish Shellfish Immunol 2020 Apr 19;99:331-341. Epub 2020 Feb 19.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.

The present study was investigated the dietary administration of cassic acid (CA) on growth, innate immunity, transcription profiles of estrogen and follicle-stimulating hormones as well as lysozyme enzyme determined in Clarias gariepinus against Edwardsiella tarda. The weight gain (WG), protein efficacy ratio (PER), and feed conversion ratio (FCR) were significantly improved in infected fish fed dietary administration with CA at 5 and 10 mg kg diets. The survival is higher (96. Read More

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Reproductive hormones influence zinc homeostasis in the bovine cumulus-oocyte complex: Impact on intracellular zinc concentration and transporters gene expression.

Theriogenology 2020 Apr 29;146:48-57. Epub 2020 Jan 29.

IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout"(UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, calles 60 y 118, B1904AMA La Plata, Buenos Aires, Argentina; Cátedra de Citología, Histología y Embriología "A" Facultad de Ciencias Médicas, Universidad Nacional de La Plata, calle 60 y 120 s/n, CP 1900, La Plata, Buenos Aires, Argentina. Electronic address:

Zinc (Zn) is a vital trace element for the body and its bioavailability influences numerous reproductive events. However, the mechanisms that regulate Zn homeostasis in the cumulus-oocyte complex (COC) are yet to be elucidated. The aim of this study was to investigate the role of estradiol 17-beta (E), FSH and LH in Zn homeostasis regulation in bovine COC matured in vitro and Zn transporters gene expression. Read More

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Induction of oocyte development in previtellogenic eel, Anguilla australis.

Gen Comp Endocrinol 2020 05 27;291:113404. Epub 2020 Jan 27.

Department of Zoology, University of Otago, 340 Great King Street, PO Box 56, Dunedin 9054, New Zealand.

The role of gonadotropins during early ovarian development in fish remains little understood. Concentrations of gonadotropins were therefore experimentally elevated in vivo by administration of recombinant follicle-stimulating hormone (rec-Fsh) or human chorionic gonadotropin (hCG) and the effects on ovarian morphology, sex steroid levels and mRNA levels of genes expressed in pituitary and ovary examined. Hormones were injected thrice at weekly intervals in different doses (20, 100 or 500 µg/kg BW for rec-Fsh and 20, 100 or 500 IU/kg BW for hCG). Read More

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Active immunization against AMH reveals its inhibitory role in the development of pre-ovulatory follicles in Zhedong White geese.

Theriogenology 2020 Mar 8;144:185-193. Epub 2020 Jan 8.

Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China; Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, 210014, China. Electronic address:

The aim of this study was to investigate the effects of active immunization against recombinant Anti-Müllerian hormone (AMH) protein on the ovarian follicular development, egg production, and molecular regulatory mechanisms in broody-prone Zhedong White geese. For this, a recombinant goose AMH protein was expressed using a prokaryotic expression system. Fifty incubating geese from the same genetic background were selected and equally divided into two groups. Read More

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Short-term but not long-term high-fat diet induces an increase in gene expression of gonadotropic hormones and GPR120 in the male mouse pituitary gland.

J Reprod Dev 2020 Apr 29;66(2):143-148. Epub 2019 Dec 29.

Laboratory of Environmental Physiology, Department of Life Science, School of Science and Engineering, Kindai University, Osaka 577-8502, Japan.

High-fat diet (HFD) is associated with the regulation of reproductive functions. This study aimed to investigate the effects of short-term HFD on the mRNA expression levels of follicle-stimulating hormone β subunit (FSHβ), luteinizing hormone β subunit (LHβ), gonadotropin-releasing hormone receptor, and long-chain fatty acid receptor, GPR120, in the matured male mouse pituitary gland. Adult male mice were fed either control chow or HFD for 1, 2, 5, 10, 30 and 150 days. Read More

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Colocalization of GPR120 and anterior pituitary hormone-producing cells in female Japanese Black cattle.

J Reprod Dev 2020 Apr 27;66(2):135-141. Epub 2019 Dec 27.

Laboratory of Environmental physiology, Department of Life Science, Kindai University, Osaka 577-8502, Japan.

Negative energy balance in domestic animals suppresses their reproductive function. These animals commonly use long-chain fatty acids (LCFAs) from adipocytes as an energy source under states of malnutrition. The G-protein coupled receptor, GPR120, is a specific receptor for LCFAs, but its role in reproductive function remains unknown in domestic animals. Read More

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Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones.

Genes (Basel) 2019 12 12;10(12). Epub 2019 Dec 12.

Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Anti-Mullerian hormone (AMH) is an important reproductive marker of ovarian reserve produced by granulosa cells (GCs) of pre-antral and early-antral ovarian follicles in several species, including cattle. This hormone plays a vital role during the recruitment of primordial follicles and follicle stimulating hormone (FSH)-dependent follicular growth. However, the regulatory mechanism of AMH expression in follicles is still unclear. Read More

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December 2019

Effects of sevoflurane on reproductive function of male rats and its main mechanism of action.

Inhal Toxicol 2019 Sep - Oct;31(11-12):392-398. Epub 2019 Dec 5.

Department of Anesthesiology, Yeda Hospital, Yantai, China.

To study the effects of sevoflurane on reproductive function and its main mechanism of action in male rats. Forty adult male Sprague-Dawley rats were divided into 4 groups and exposed to 0, 50, 300 and 1800 ppm of sevoflurane, respectively. After 15 days, the serum levels of sex hormones and inflammatory factors were detected using enzyme-linked immunosorbent assay. Read More

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The regulation and signalling of anti-Müllerian hormone in human granulosa cells: relevance to polycystic ovary syndrome.

Hum Reprod 2019 12;34(12):2467-2479

Cell Biology and Genetics Research Centre, St George's University of London, London SW17 0RE, UK.

Study Question: What prevents the fall in anti-Müllerian hormone (AMH) levels in polycystic ovary syndrome (PCOS) and what are the consequences of this for follicle progression in these ovaries?

Summary Answer: Exposure of granulosa cells (GCs) to high levels of androgens, equivalent to that found in PCOS, prevented the fall in AMH and was associated with dysregulated AMH-SMAD signalling leading to stalled follicle progression in PCOS.

What Is Known Already: In normal ovaries, AMH exerts an inhibitory role on antral follicle development and a fall in AMH levels is a prerequisite for ovulation. Levels of AMH are high in PCOS, contributing to the dysregulated follicle growth that is a common cause of anovulatory infertility in these women. Read More

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December 2019

The mRNA expression patterns of kisspeptins, GnRHs, and gonadotropins in the brain and pituitary gland of a tropical damselfish, Chrysiptera cyanea, during the reproductive cycle.

Fish Physiol Biochem 2020 Feb 8;46(1):277-291. Epub 2019 Nov 8.

Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru 1, Nishihara, Okinawa, 903-0213, Japan.

The sapphire devil (Chrysiptera cyanea) is a tropical damselfish that undergoes active reproduction under long-day conditions. To elucidate the physiological regulation of the brain-pituitary-gonadal axis in female sapphire devil, we cloned and characterized the genes of two kisspeptins (kiss1 and kiss2), three gonadotropin-releasing hormones (gnrh1, gnrh2, gnrh3), and the β-subunit of two gonadotropins (fshβ and lhβ) and investigated the gene expression changes during ovarian development. Quantitative polymerase chain reaction analyses in various brain parts revealed high expression levels of kiss1, kiss2, and gnrh2 in the diencephalon; gnrh2 and gnrh3 in the telencephalon; and fshβ and lhβ in the pituitary. Read More

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February 2020