124,479 results match your criteria frozen


Spinous process screw fixation: A salvage technique in sub-axial cervical spinal instrumentation.

World Neurosurg 2021 Jul 19. Epub 2021 Jul 19.

Department of Orthopaedics, Peking University Third Hospital, Beijing, China. Electronic address:

Objective: To evaluate the feasibility and efficacy of spinous process screws (SPS) in sub-axial cervical fixation.

Methods: A retrospective study was conducted on seven patients receiving posterior cervical instrumentation for sub-axial fracture dislocation or atlantoaxial dislocation from 2014 to 2015. Additionally, a biomechanical test was performed on seven fresh-frozen cadaveric spine samples (from the occiput to C7) from healthy human subjects with no history of spinal trauma. Read More

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Enteric adenovirus epidemiology from historical fecal samples in Brazil (1998-2005): Pre-rotavirus vaccine era.

Infect Genet Evol 2021 Jul 19:105007. Epub 2021 Jul 19.

Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, Sao Paulo, Brazil. Electronic address:

Human adenovirus (HAdV) is recognized as frequent cause of acute gastroenteritis and enteric viruses can be preserved in frozen stored feces for long periods of times. The purpose of the present study was to investigate enteric HAdV genotypic diversity in archival fecal specimens stored from 1998 to 2005 in order to understand the natural history of HAdV in diarrheal patients in Brazil before rotavirus vaccine introduction. A total of 3346 specimens were tested for HAdV using conventional PCR. Read More

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The Effect of Hemiarthroplasty Implant Modulus on Contact Mechanics: An Experimental Investigation.

J Shoulder Elbow Surg 2021 Jul 19. Epub 2021 Jul 19.

Department of Mechanical and Materials Engineering, Western University, London, ON, Canada; Bioengineering Laboratory, Roth McFarlane Hand and Upper Limb Centre, Lawson Health Research Institute, St Joseph's Health Care, London, ON, Canada; Department of Surgery, Western University, London, ON, Canada. Electronic address:

Background: Hemiarthroplasties cause damage to the cartilage that they articulate against, which is a major limitation to their use. This study investigates the use of lower stiffness materials to determine if they improve hemiarthroplasty contact mechanics and thus reduce the risk of cartilage damage.

Methods: Eleven fresh-frozen cadaveric upper extremities were disarticulated and fixed in a custom-built jig that applied a static load of 50 N to the radiocapitellar joint. Read More

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A Low-Sodium DASH Dietary Pattern Affects Serum Markers of Inflammation and Mineral Metabolism in Adults with Elevated Blood Pressure.

J Nutr 2021 Jul 22. Epub 2021 Jul 22.

Welch Center for Prevention, Epidemiology, and Clinical Research, Johns Hopkins University, Baltimore, MD, USA.

Background: The blood pressure-lowering effects of the Dietary Approaches to Stop Hypertension (DASH) dietary pattern and reduced sodium intake are well established. The effects on other biomarkers related to vascular health are of interest and might assist in explaining the effects of the DASH diet and sodium reduction.

Objectives: We hypothesized that a low-sodium DASH diet improves (lowers) biomarkers of inflammation [C-reactive protein (CRP) and soluble urokinase plasminogen activator receptor (suPAR)] and mineral metabolism [phosphorus and fibroblast growth factor-23 (FGF23)]. Read More

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The Comma Sign: An Anatomic Study.

Orthopedics 2021 Jul-Aug;44(4):e546-e548. Epub 2021 Jul 1.

The "comma sign" is a comma-shaped arc of tissue located at the superolateral edge of the subscapularis at its insertion on the humeral head. It consists of the coracohumeral ligament (CHL) and the superior glenohumeral ligament (SGHL). It was recently recognized as an important landmark to aid surgeons in identifying the edge of a torn subscapularis tendon. Read More

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Posterior Cruciate Ligament Reconstruction With Internal Brace Augmentation Reduces Posterior Tibial Translation Under Cyclic Loading.

Orthopedics 2021 Jul-Aug;44(4):235-240. Epub 2021 Jul 1.

The goal of this study was to evaluate the stiffness and resistance to elongation of an internal bracing (IB) construct in posterior cruciate ligament reconstruction (PCLR). The authors hypothesized that augmentation with an internal brace would increase construct stiffness and decrease posterior tibial translation during cyclic loading in a fresh frozen cadaveric model. Ten cadaver knees underwent PCL reconstruction with (PCLR+IB) and without (PCLR) augmentation with an internal brace and were compared with an intact PCL state. Read More

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Causes of an elevated international normalized ratio in the intensive care unit and the implications for plasma transfusion.

Transfusion 2021 Jul 22. Epub 2021 Jul 22.

Department of Coagulation and Transfusion Medicine, The Miriam Hospital, Providence, Rhode Island, USA.

Background: The presence of an elevated international normalized ratio (INR) is common in patients in the intensive care unit (ICU), but the cause rarely determined. These patients are at risk to receive prophylactic plasma prior to invasive procedures.

Study Design And Methods: Samples from patients with an INR of 1. Read More

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Compliance with sentinel lymph node biopsy guidelines for invasive melanomas treated with Mohs micrographic surgery.

Cancer 2021 Jul 22. Epub 2021 Jul 22.

Department of Dermatology, University of Pennsylvania Health System, Philadelphia, Pennsylvania.

Background: Sentinel lymph node biopsy (SLNB) has not been studied for invasive melanomas treated with Mohs micrographic surgery using frozen-section MART-1 immunohistochemical stains (MMS-IHC). The primary objective of this study was to assess the accuracy and compliance with National Comprehensive Cancer Network (NCCN) guidelines for SLNB in a cohort of patients who had invasive melanoma treated with MMS-IHC.

Methods: This retrospective cohort study included all patients who had primary, invasive, cutaneous melanomas treated with MMS-IHC at a single academic center between March 2006 and April 2018. Read More

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Strong association between insufficient plasma exchange and fatal outcomes in Japanese patients with immune-mediated thrombotic thrombocytopenic purpura.

Int J Hematol 2021 Jul 22. Epub 2021 Jul 22.

Department of Blood Transfusion Medicine, Nara Medical University, 840 Shijo-Cho, Kashihara, 634-8522, Japan.

Plasma exchange (PEX) using fresh frozen plasma has considerably reduced the mortality rate in patients with immune-mediated thrombotic thrombocytopenic purpura (iTTP). However, some patients still do not survive even with treatment, but little information is available regarding which treatment these patients received. This study was conducted to obtain this information in 240 patients who met the current iTTP diagnostic criteria and completed at least 30 days of follow-up except for deceased cases. Read More

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Effectiveness of proprioceptive training and conventional physical therapy in treating adhesive capsulitis.

Pak J Med Sci 2021 Jul-Aug;37(4):1196-1200

Sadaf Aziz, DPT, MSPT. Assistant Professor, Rehman College of Rehabilitation Sciences, Peshawar, Pakistan.

Objective: To determine effectiveness of proprioceptive training and conventional physical therapy in managing patients with adhesive capsulitis.

Methods: A pre-test post-test control group study was conducted at Rehman Medical Institute from June to December 2019. Thirty-eight patients, aged 30 to 60 years, with diagnosis of adhesive capsulitis for more than four months were divided into two groups. Read More

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November 2020

Minocycline Treatment Reduces Mass and Force Output From Fast-Twitch Mouse Muscles and Inhibits Myosin Production in C2C12 Myotubes.

Front Physiol 2021 5;12:696039. Epub 2021 Jul 5.

School of Medicine, Western Sydney University, Sydney, NSW, Australia.

Minocycline, a tetracycline-class of antibiotic, has been tested with mixed effectiveness on neuromuscular disorders such as amyotrophic lateral sclerosis, autoimmune neuritis and muscular dystrophy. The independent effect of minocycline on skeletal muscle force production and signalling remain poorly understood. Our aim here is to investigate the effects of minocycline on muscle mass, force production, myosin heavy chain abundance and protein synthesis. Read More

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Glycosphingolipid GM3 is localized in both exoplasmic and cytoplasmic leaflets of Plasmodium falciparum malaria parasite plasma membrane.

Sci Rep 2021 Jul 21;11(1):14890. Epub 2021 Jul 21.

Department of Molecular and Cell Biology and Biochemistry, Basic Veterinary Science, Faculty of Veterinary Medicine, Kagoshima University, Korimoto 1-21-24, Kagoshima, 890-0065, Japan.

Lipid rafts, sterol-rich and sphingolipid-rich microdomains on the plasma membrane are important in processes like cell signaling, adhesion, and protein and lipid transport. The virulence of many eukaryotic parasites is related to raft microdomains on the cell membrane. In the malaria parasite Plasmodium falciparum, glycosylphosphatidylinositol-anchored proteins, which are important for invasion and are possible targets for vaccine development, are localized in the raft. Read More

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Post-thaw culture has no beneficial effect on frozen embryos transfer outcomes comparing to same embryonal age non-cultured frozen-thawed embryos.

J Matern Fetal Neonatal Med 2021 Jul 21:1-8. Epub 2021 Jul 21.

Fertility and IVF Unit, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Purpose: To assess the impact of post-thawing embryo culture on frozen embryo transfer (FET) outcomes.

Methods: A retrospective cohort study including 678 consecutive FET cycles performed between the years 2004 and 2017 was conducted. Patients older than 45 years old were excluded. Read More

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Biomechanical evaluation of an intramedullary clavicle screw in simple oblique and butterfly wedge fractures.

Proc Inst Mech Eng H 2021 Jul 21:9544119211031052. Epub 2021 Jul 21.

Medical College of Georgia at Augusta University, Augusta, GA, USA.

This biomechanical study evaluates the performance of a solid titanium-alloy intra-medullary () clavicular screw in torsion and cantilever bending in cadaveric clavicle specimens with simulated simple oblique and butterfly wedge midshaft fractures. Thirty-two fresh-frozen male clavicles were sorted into six experimental groups: Torsion Control, Torsion Simple Oblique Fracture, Torsion Butterfly Wedge Fracture, Bending Control, Bending Simple Oblique Fracture, and Bending Butterfly Wedge Fracture. The experimental groups were controlled for density, length, diameter, and laterality. Read More

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Melatonin improves learning and memory of mice with chronic social isolation stress via an interaction between microglia polarization and BDNF/TrkB/CREB signaling pathway.

Eur J Pharmacol 2021 Jul 18;908:174358. Epub 2021 Jul 18.

Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran; Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Chronic social isolation stress (SIS) could impair learning and memory-related behaviors. Herein, we investigated the efficacy of Melatonin in treatment of memory despair and also its possible underlying mechanism of action in an animal model of SIS. For this purpose, mice were allocated to two opposing conditions, including social condition (SC) and isolated condition (IC), for five weeks. Read More

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Comparison of procedural efficacy, balloon nadir temperature and incidence of phrenic nerve palsy between two cryoballoon technologies for pulmonary vein isolation: a systematic review and meta-analysis.

J Cardiovasc Electrophysiol 2021 Jul 21. Epub 2021 Jul 21.

Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands.

Introduction: In May 2020 a novel cryoballoon system (POLARx, Boston Scientific) became available for catheter ablation of atrial fibrillation (AF). The design of the cryoballoon is comparable to the Arctic Front Advance Pro (AFA-Pro, Medtronic), but it is more compliant during freezing. We compared the procedural efficacy, biophysical parameters and risk of phrenic nerve palsy (PNP) between the two cryoballoons. Read More

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Eight metagenome-assembled genomes provide evidence for microbial adaptation in 20,000 to 1,000,000-year-old Siberian permafrost.

Appl Environ Microbiol 2021 Jul 21:AEM0097221. Epub 2021 Jul 21.

University of Tennessee, Knoxville Tennessee 37996 USA.

Permafrost microbes may be metabolically active in microscopic layers of liquid brines, even in ancient soil. Metagenomics can help discern whether permafrost microbes show adaptations to this environment. Thirty-three metagenome-assembled genomes (MAGs) were obtained from six depths (3. Read More

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Feasibility of lateral sentinel lymph node biopsy in medullary thyroid cancer: A surrogate tool for determining prophylactic lateral neck dissection-A pilot study.

Head Neck 2021 Jul 20. Epub 2021 Jul 20.

Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.

Backgrounds: This study aimed to evaluate usefulness of lateral sentinel lymph node biopsy (SLNB) in determining lateral neck dissection (LND) of patients with medullary thyroid cancer (MTC).

Methods: Sixteen patients with MTC were enrolled in the study from January 2013 to June 2019. Intratumoral injection of technetium (Tc)-99m phytate followed by lymphoscintigraphy was performed preoperatively. Read More

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Assessing growth, frost tolerance and acclimation of pine seedlings with contrasted dormancy strategies as influenced by organic nitrogen supply.

Physiol Plant 2021 Jul 20. Epub 2021 Jul 20.

Forest Ecology and Restoration Group. Departamento de Ciencias de la Vida. Universidad de Alcalá. Apdo, Madrid, Spain.

Freezing stress is a critical environmental factor affecting survival, distribution, and evolution of plants. Although there is evidence that nitrogen (N) affects frost tolerance of juvenile conifers, the magnitude and direction of such effect can diverge among species. The influence of the N source on frost tolerance has been barely studied. Read More

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Linezolid- and Multidrug-Resistant Enterococci in Raw Commercial Dog Food, Europe, 2019-2020.

Emerg Infect Dis 2021 Aug;27(8):2221-2224

We describe enterococci in raw-frozen dog food commercialized in Europe as a source of genes encoding resistance to the antibiotic drug linezolid and of strains and plasmids enriched in antibiotic-resistance and virulence genes in hospitalized patients. Whole-genome sequencing was fundamental to linking isolates from dog food to human cases across Europe. Read More

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Ultrastructural analysis of Lyophilized Human Spermatozoa.

JBRA Assist Reprod 2021 Jul 21;25(3):473-479. Epub 2021 Jul 21.

Department of Obstetrics and Gynaecology of the Medical School, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

Objective: Lyophilization is potentially more practical and cost-effective alternative for sperm preservation. However, there are no studies that evaluate the ultrastructure of human spermatozoa after lyophilization. Therefore, the aim of our study was to evaluate the ultrasctructure of lyophilized spermatozoa using Transmission Electron Microscopy. Read More

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Structure and thermodynamics of empty clathrate hydrates below the freezing point of water.

Phys Chem Chem Phys 2021 Jul 21. Epub 2021 Jul 21.

LAQV-REQUIMTE, Department of Chemistry, Faculdade de Ciências e Tecnologia, NOVA University Lisbon, 2829-516 Caparica, Portugal.

Recently prepared as a new H2O phase, ice XVI was obtained by degassing a Ne-sII clathrate hydrate under vacuum, however very little is known of that crystalline solid under temperatures (T ≤ 220 K) and pressures (p ≤ 5000 bar) relevant for the Earth's environment and geochemistry. In this work, atomically detailed calculations using long time-scale molecular simulations, seldom paralelled before, are employed to probe empty sII clathrate hydrates. It is found that the volumetric response to an applied pressure-temperature gradient is accurately described by the Parsafar and Mason equation of state with an accuracy of at least 99. Read More

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Mild Acquired Factor XIII Deficiency and Clinical Relevance at the ICU-A Retrospective Analysis.

Clin Appl Thromb Hemost 2021 Jan-Dec;27:10760296211024741

Institute of IMD Blood Coagulation Centre, Frankfurt/Bad Homburg, Germany.

Acquired FXIII deficiency is a relevant complication in the perioperative setting; however, we still have little evidence about the incidence and management of this rarely isolated coagulopathy. This study aims to help find the right value for the substitution of patients with an acquired mild FXIII deficiency. In this retrospective single-center cohort study, we enrolled critically ill patients with mild acquired FXIII deficiency (>5% and ≤70%) and compared clinical and laboratory parameters, as well as pro-coagulatory treatments. Read More

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Liver biomarkers response of the neotropical fish to environmental stressors associated with the oil industry.

Heliyon 2021 Jul 1;7(7):e07458. Epub 2021 Jul 1.

Grupo de Investigación en Biotecnología y Toxicología Acuática y Ambiental - BioTox, Facultad de Ciencias Agrícolas y Recursos Naturales, Universidad de los Llanos, km 12 vía Puerto López, vereda Barcelona, Villavicencio, Colombia.

The Acacias River in Colombia receives large volumes of industrial effluents mostly derived from the oil industry. To contribute to the study of the possible effects of industrial wastewaters on the aquatic environment and particularly on fish populations, a native neotropical fish, was used as a sentinel species. Wild specimens of were caught at three different places of the Acacias River taking as reference the point of discharge of an oil industry effluent; upstream, downstream, and at the vicinity of the discharge pipe. Read More

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Oocytes Preparation for in-Cell EPR Spectroscopy.

Bio Protoc 2018 Apr 5;8(7):e2798. Epub 2018 Apr 5.

Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany.

One of the most exciting perspectives for studying bio-macromolecules comes from the emerging field of in-cell spectroscopy, which enables to determine the structure and dynamics of bio-macromolecules in the cell. In-cell electron paramagnetic resonance (EPR) spectroscopy in combination with micro-injection of bio-macromolecules into oocytes is ideally suited for this purpose. oocytes are a commonly used eukaryotic cell model in different fields of biology, such as cell- and development-biology. Read More

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A review of coral bleaching specimen collection, preservation, and laboratory processing methods.

PeerJ 2021 8;9:e11763. Epub 2021 Jul 8.

School of Earth Sciences, Ohio State University, Columbus, OH, United States of America.

Under current climate warming predictions, the future of coral reefs is dire. With projected coral reef decline, it is likely that coral specimens for bleaching research will increasingly become a more limited resource in the future. By adopting a holistic approach through increased collaborations, coral bleaching scientists can maximize a specimen's investigative yield, thus reducing the need to remove more coral material from the reef. Read More

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Liver Protection Mechanism and Absorption Promotion Technology of Silybin Based on Intelligent Medical Analysis.

J Healthc Eng 2021 3;2021:9968016. Epub 2021 Jul 3.

Zhucheng People's Hospital, Zhucheng 262200, Shandong, China.

With the continuous popularization of smart medicine, the protective effect of silibinin in the liver has attracted much attention. This study mainly explores the liver protection mechanism and absorption promotion technology of silybin based on intelligent medical analysis. Refining of silibinin: accurately weigh 1. Read More

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Barcode medication administration technology use in hospital practice: a mixed-methods observational study of policy deviations.

BMJ Qual Saf 2021 Jul 20. Epub 2021 Jul 20.

Department of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway.

Introduction: Barcode medication administration (BCMA) can, if poorly implemented, cause disrupted workflow, increased workload and cause medication errors. Further exploration is needed of the causes of BCMA policy deviations.

Objective: To gain an insight into nurses' use of barcode technology during medication dispensing and administration; to record the number and type of BCMA policy deviations, and to investigate their causes. Read More

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Attention switching deficit in patients of Parkinson's disease who experience freezing of gait.

Appl Neuropsychol Adult 2021 Jul 20:1-12. Epub 2021 Jul 20.

Psychophysiology Laboratory, Department of Humanities and Social Sciences, Indian Institute of Technology Bombay, Mumbai, India.

Attention switching is involved in postural adjustments for gait. A deficit in attention switching was expected among patients having Parkinson's disease and experiencing freezing. There was a deficit in attention switching abilities among the patients of Parkinson's disease, having episodes of freezing of gait. Read More

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