2,478 results match your criteria freezing prior

Transcriptional response to prolonged perchlorate exposure in the methanogen Methanosarcina barkeri and implications for Martian habitability.

Sci Rep 2021 Jun 11;11(1):12336. Epub 2021 Jun 11.

Department of Geosciences, Princeton University, Princeton, NJ, USA.

Observations of trace methane (CH) in the Martian atmosphere are significant to the astrobiology community given the overwhelming contribution of biological methanogenesis to atmospheric CH on Earth. Previous studies have shown that methanogenic Archaea can generate CH when incubated with perchlorates, highly oxidizing chaotropic salts which have been found across the Martian surface. However, the regulatory mechanisms behind this remain completely unexplored. Read More

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Development of a Home Meal Replacement Product Containing Braised Mackerel () with Radish ().

Foods 2021 May 19;10(5). Epub 2021 May 19.

Seafood Research Center, IACF, Silla University, 606, Advanced Seafood Processing Complex, Wonyang-ro, Amnam-dong, Seo-gu, Busan 49277, Korea.

The coronavirus disease pandemic has contributed to increasing convenience in food preferences. Home meal replacement (HMR) products are ready-to-eat, -cook, or -heat foods, providing convenience for consumers. We developed a HMR product containing mackerel as a protein- and lipid-rich source using various food-processing technologies to maintain its nutritional content and prolong shelf life. Read More

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Validating a Seated Virtual Reality Threat Paradigm for Inducing Anxiety and Freezing of Gait in Parkinson's Disease.

J Parkinsons Dis 2021 May 28. Epub 2021 May 28.

Parkinson's Disease Research Clinic, Brain and Mind Centre, University of Sydney, Australia.

Background: Although prior research has established that freezing of gait (FOG) in Parkinson's disease (PD) is associated with anxiety, only one study to date has directly manipulated anxiety levels to induce FOG.

Objective: The current study aimed to replicate these previous findings and evaluate whether a seated version of a 'threat' virtual reality (VR) paradigm could induce anxiety and provoke FOG.

Methods: Twenty-four PD patients with FOG were assessed across various threat conditions in both a walking VR paradigm (Experiment 1) and a seated VR paradigm (Experiment 2). Read More

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Establishment of a novel cell-based assay using HLA-transfected cells to detect HLA antibodies.

J Immunol Methods 2021 May 26;495:113074. Epub 2021 May 26.

Japanese Red Cross Hokkaido Blood Center, Sapporo, Hokkaido, Japan.

The detection of HLA antibodies is important in clinical practice, such as platelet transfusion refractoriness and transfusion-related lung injury. However, difficulties are associated with the preparation of panel cells for conventional HLA detection systems using intact cells, such as the immunocomplex capture fluorescence analysis (ICFA). Based on an ICFA analysis, HEK293 cells stably transfected with the HLA-A locus were used instead of peripheral blood mononuclear cells (PBMC). Read More

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Bone fragment or bone powder? ATR-FTIR spectroscopy-based comparison of chemical composition and DNA preservation of bones after 10 years in a freezer.

Int J Legal Med 2021 May 24. Epub 2021 May 24.

National Institute of Chemistry, Hajdrihova 19, 1000, Ljubljana, Slovenia.

Freezing bone samples to preserve their biomolecular properties for various analyses at a later time is a common practice. Storage temperature and freeze-thaw cycles are well-known factors affecting degradation of molecules in the bone, whereas less is known about the form in which the tissue is most stable. In general, as little intervention as possible is advised before storage. Read More

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Genetic mapping and identification of a QTL determining tolerance to freezing stress in Fragaria vesca L.

PLoS One 2021 21;16(5):e0248089. Epub 2021 May 21.

Department of Genetics, Genomics and Breeding, NIAB-EMR, East Malling, Kent, United Kingdom.

Extreme cold and frost cause significant stress to plants which can potentially be lethal. Low temperature freezing stress can cause significant and irreversible damage to plant cells and can induce physiological and metabolic changes that impact on growth and development. Low temperatures cause physiological responses including winter dormancy and autumn cold hardening in strawberry (Fragaria) species, and some diploid F. Read More

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Short/long-term cryopreservation prior to comet assay of whole-blood leukocytes and -cultured lung fibroblasts.

Toxicol Mech Methods 2021 May 20:1-25. Epub 2021 May 20.

Institute of Biology, Ecology and Natural Resources, Kemerovo State University, Kemerovo, Russian Federation.

Single-cell gel electrophoresis (comet assay) is a valuable test that can be used in ecotoxicological, epidemiological, and biomonitoring contexts. We assessed the effects of short- (without cryopreservation) and long-term (with cryopreservation) storage of DMEM-cultivated human peripheral blood leukocytes (HPBLs) and a human lung fibroblast cell line (FLECH-104) on comet assay results. Samples were stored for 6 or 24 h at room temperature (23°С) or 4 °C and frozen at -80 °C or -196 °C for 1, 2, or 4 weeks. Read More

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Ventral hippocampus mediates the context-dependence of two-way signaled avoidance in male rats.

Neurobiol Learn Mem 2021 May 18;183:107458. Epub 2021 May 18.

Texas A&M University Institute for Neuroscience, Texas A&M University, College Station, TX 77843-4235, United States; Department of Psychological & Brain Sciences, Texas A&M University, College Station, TX 77843-4235, United States. Electronic address:

Considerable work indicates that instrumental responding is context-dependent, but the neural mechanisms underlying this phenomenon are poorly understood. Given the important role for the hippocampal formation in contextual processing, we hypothesized that reversible inactivation of the hippocampus would impair the context-dependence of active avoidance. To test this hypothesis, we used a two-way signaled active avoidance (SAA) task that requires rats to shuttle across a divided chamber during a tone CS in order to avoid a footshock US. Read More

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Comparison of two methods for prolong storage of decellularized mouse whole testis for tissue engineering application: An experimental study.

Int J Reprod Biomed 2021 Apr 22;19(4):321-332. Epub 2021 Apr 22.

Basic Medical Science Research Center, Histogenotech Company, Tehran, Iran.

Background: Biological scaffolds are derived by the decellularization of tissues or organs. Various biological scaffolds, such as scaffolds for the liver, lung, esophagus, dermis, and human testicles, have been produced. Their application in tissue engineering has created the need for cryopreservation processes to store these scaffolds. Read More

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Dimethylacetamide in Rooster Semen Cryopreservation.

Cryo Letters 2021 Jan-Feb;42(1):39-43

Reproducao Animal Comparada, Instituto de Ciencia Biologicas, Universidade Federal de Rio Grande, Rio Grande, Brasil.

Background: Sperm cryopreservation of cockerels is a major challenge, and so far there is no adequate information to enable commercial use of frozen semen.

Objective: To test the toxicity of dimethylacetamide (DMA).

Materials And Methods: DMA was added at 3%, 6%, 9% and 12% to the freezing diluent, and maintained for equilibration with the semen sample for 1 min, 3 min, 5 min, 7 min and 9 min prior to freezing. Read More

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Vitrification and Ultra-Rapid Laser Warming of Yeast Saccharomyces cerevisiae.

E Paredes

Cryo Letters 2021 Jan-Feb;42(1):19-24

Centro de Investigacion Mariña, Universidade de Vigo, Laboratorio de Ecoloxia Costeira (ECOCOST), Departamento de Ecoloxia e Bioloxia Animal, Vigo, Spain.

Background: As fundamental model organisms, yeasts have been used for the study and understanding of cryopreservation and freezing damage mechanisms, in particular Saccharomyces cerevisiae.

Objective: As cryopreservation success requires optimization of the cooling and warming rates, the objective was to test how ultra-rapid warming could improve yeast cell cryopreservation.

Materials And Methods: S. Read More

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Do animal slaughter age and pre-cure freezing have a significant impact on the quality of Iberian dry-cured pork loin?

Meat Sci 2021 Sep 1;179:108531. Epub 2021 May 1.

Scientific and Technological Research Centre of Extremadura (CICYTEX-La Orden), Meat Quality Area, Junta de Extremadura, Ctra. A-V, Km372, 06187 Guadajira, Badajoz, Spain.

The influence of various animal slaughter age and of pre-cure freezing times on the quality traits of dry-cured loins from Iberian × Duroc (IBxD) crossbreed animals was assessed. Three animal batches (n = 15) were selected (IBxD8, IBxD10 and IBxD12) to be slaughtered at the age of 8, 10 and 12 months, respectively. Following slaughter, longissimus thoracis et lumborum muscles were extracted and submitted to curing (T0) or freezing storage during three (T3) or six (T6) months prior to curing. Read More

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September 2021

Exposure to epididymal extracellular vesicles enhances immature sperm function and sustains vitality of cryopreserved spermatozoa in the domestic cat model.

J Assist Reprod Genet 2021 May 5. Epub 2021 May 5.

Smithsonian Conservation Biology Institute, National Zoological Park, Washington, DC, USA.

Purpose: Extracellular vesicles (EVs) secreted by the epididymal epithelium transfer key factors to maturing spermatozoa. Using an in vitro system previously developed in our laboratory, the objective was to (1) characterize the impact of EV exposure on the fertilizing ability and developmental potential of immature sperm cells from the caput epididymidis and (2) examine the benefit of EV exposure to restore vitality of mature spermatozoa from the cauda epididymidis after freezing-thawing.

Methods: EVs were isolated from entire epididymides and collected into pellets via ultracentrifugation. Read More

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Current approaches of preservation of cells during (freeze-) drying.

J Pharm Sci 2021 Apr 30. Epub 2021 Apr 30.

Ludwig-Maximilians-Universität München, Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Munich, Germany.

The widespread application of therapeutic cells requires a successful stabilization of cells for the duration of transport and storage. Cryopreservation is currently considered the gold standard for the storage of active cells; however, (freeze-) drying cells could enable higher shelf life stability at ambient temperatures and facilitate easier transport and storage. During (freeze-) drying, freezing, (primary and secondary) drying and also the reconstitution step pose the risk of potential cell damage. Read More

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Observer's adrenal corticosterone secretion involvement in vicarious fear conditioning.

Psychoneuroendocrinology 2021 Apr 26;129:105246. Epub 2021 Apr 26.

Department of Physiology, National Cheng Kung University College of Medicine, Tainan 701, Taiwan, ROC; Institute of Basic Medical Sciences, National Cheng Kung University College of Medicine, Tainan 701, Taiwan, ROC. Electronic address:

Vicarious learning represents a far-reaching value for the survival of social animals. Adrenal hormones are known to affect many forms of learning, yet the roles of adrenal hormones in vicarious learning remain unexplored. This study was undertaken to assess whether observation-stimulated corticosterone (CORT) secretion may affect the magnitude of a vicarious fear conditioning. Read More

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Stress-induced impairment of fear extinction recall is associated with changes in neuronal activity patterns in PVT.

Prog Neuropsychopharmacol Biol Psychiatry 2021 Apr 26;111:110338. Epub 2021 Apr 26.

Institute of Physiology I, Westfälische Wilhelms-University Münster, 48149 Münster, Germany. Electronic address:

Treatment resistance of anxiety-related disorders often arises from an inappropriate fear expression, impairment in fear extinction, and spontaneous return of fear. Stress exposure is considered a high risk factor for neuropsychiatric disorders, but understanding of the long-term consequences of stress is limited, particularly when it comes to treatment outcome. Therefore, studying the consequences of acute stress would provide critical information on the role of stress in psychopathology. Read More

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Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy.

Oncol Lett 2021 Jun 8;21(6):458. Epub 2021 Apr 8.

Department of Basic Medical Sciences, Royal College of Surgeons in Ireland, Medical University of Bahrain, Adliya 15503, Kingdom of Bahrain.

Cryoablation is an emerging type of treatment for cancer. The sensitization of tumors using cryosensitizing agents prior to treatment enhances ablation efficiency and may improve clinical outcomes. Water efflux, which is regulated by aquaporin channels, contributes to cancer cell damage achieved through cryoablation. Read More

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Forensic analysis on bursting accident of buffer tank in liquefied natural gas bus.

J Forensic Sci 2021 Apr 27. Epub 2021 Apr 27.

Ningbo Special Equipment Inspection & Research Institute, Ningbo, China.

In December 2019, a buffer tank burst accident occurred in the maintenance of liquefied natural gas (LNG) bus in China. Failure analysis revealed the bus-mounted buffer tank had been subjected to an excessive internal pressurization, although the tank material met the specifications for engineering practice. The physical evidence related to the failed tank showed that a chemical explosion was impossible. Read More

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The impact of adenomyosis on IVF outcomes: a prospective cohort study.

Hum Reprod Open 2021 19;2021(2):hoab015. Epub 2021 Apr 19.

Newlife IVF, Box Hill, VIC, Australia.

Study Question: Does the presence of adenomyosis in women treated with IVF alter IVF outcomes?

Summary Answer: Adenomyosis does not significantly alter IVF outcomes when adjusted for confounding factors including maternal age and smoking status.

What Is Known Already: Studies evaluating adenomyosis and its impact on infertility, particularly when focusing on IVF, remain controversial. Many studies report that adenomyosis has a detrimental effect on IVF outcomes, however age is strongly related with both the prevalence of adenomyosis and worse reproductive outcomes. Read More

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A novel method for the storage and transport of biological samples of therapeutic proteins prior to the detection of analytes using ELISA.

Sci Rep 2021 Apr 22;11(1):8763. Epub 2021 Apr 22.

Research Institute of Drug Metabolism and Pharmacokinetics, School of Xiangya Pharmaceutical Sciences, Central South University, Changsha, 410013, Hunan, China.

Therapeutic proteins have exhibited promising clinical applications in the diagnosis and treatment of some diseases. Prior to the detection of analytes using enzyme-linked immunosorbent assay, biological samples of therapeutic proteins are conventionally frozen at temperatures ranging from - 20 to - 80 °C to increase the stability of analytes. However, therapeutic proteins destabilization and aggregation may occur during the frozen storage or the freeze-thawing step. Read More

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Cold tolerance of laboratory-reared Asian longhorned beetles.

Comp Biochem Physiol A Mol Integr Physiol 2021 Jul 20;257:110957. Epub 2021 Apr 20.

Department of Biology, The University of Western Ontario, London, ON N6A 5B7, Canada.

Low winter temperatures in temperate climates can limit the success of non-native species. The Asian longhorned beetle, Anoplophora glabripennis, is an invasive wood-boring pest of hardwood trees in North America and Europe. Native A. Read More

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Allogeneic transplant procurement in the times of COVID-19: Quality report from the central European cryopreservation site.

J Transl Med 2021 04 8;19(1):145. Epub 2021 Apr 8.

German Red Cross Blood Service Baden-Württemberg-Hesse, Institute Frankfurt, Frankfurt a.M., Germany.

Background: Because of limitations of transportation imposed by the COVID-19 pandemic, current recommendation calls for cryopreservation of allogeneic stem cell transplants before patient conditioning. A single cell therapy laboratory was selected to function as the central cryopreservation hub for all European registry donor transplants intended for the Australian-Pacific region. We examined properties of these transplants to ascertain how quality is maintained. Read More

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Early Detection of Freezing of Gait during Walking Using Inertial Measurement Unit and Plantar Pressure Distribution Data.

Sensors (Basel) 2021 Mar 23;21(6). Epub 2021 Mar 23.

Department of Systems Design Engineering, University of Waterloo, Waterloo, ON N2L 3G1, Canada.

Freezing of gait (FOG) is a sudden and highly disruptive gait dysfunction that appears in mid to late-stage Parkinson's disease (PD) and can lead to falling and injury. A system that predicts freezing before it occurs or detects freezing immediately after onset would generate an opportunity for FOG prevention or mitigation and thus enhance safe mobility and quality of life. This research used accelerometer, gyroscope, and plantar pressure sensors to extract 861 features from walking data collected from 11 people with FOG. Read More

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Abnormal theta oscillation aggravated by chronic stress in the CA1 may mediate the deterioration of fear memory impairment induced by lipopolysaccharide.

Brain Res Bull 2021 Jun 19;171:172-182. Epub 2021 Mar 19.

Department of Anesthesiology, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, China. Electronic address:

Both environmental stress and immune challenge can induce abnormal neurobehavior. However, the impact of chronic stress on immune challenge-related neurobehavioral abnormalities is still controversial. Hence, we aimed to investigate the effects of chronic stress on immune challenge-related neurobehavioral abnormalities and explore the possible underlying mechanisms. Read More

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Ovarian stimulation for oocyte donation: a systematic review and meta-analysis.

Hum Reprod Update 2021 Mar 20. Epub 2021 Mar 20.

Department of Obstetrics, Gynaecology and Reproductive Medicine, Hospital Universitario Dexeus, Barcelona, Spain.

Background: Since its introduction in the 1980s, oocyte donation (OD) has been largely integrated into ART. Lately, both demand and the indications for OD have increased greatly. Oocyte donors are healthy and potentially fertile women undergoing voluntarily ovarian stimulation (OS). Read More

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Strategies to improve intratrain prospective motion correction for turbo spin-echo sequences with constant flip angles.

Magn Reson Med 2021 08 16;86(2):852-865. Epub 2021 Mar 16.

Department of Radiology, Medical Physics, Medical Center University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Purpose: To investigate the effects of prospective motion correction on turbo spin echo sequences and optimize motion correction approaches, mitigating signal dropout artifacts caused by the imperfections of motion tracking data.

Methods: Signal dropout artifacts caused by undesired phase deviations introduced by tracking errors are analyzed theoretically. To reduce the adverse effect of such deviations, two approaches are proposed: (1) freezing the correction for example, for even-numbered or higher number of echoes and (2) shifting the correction event prior to the left crusher gradient preceding the refocusing pulse. Read More

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High-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes.

Methods Cell Biol 2021 2;162:151-170. Epub 2020 Oct 2.

Experimental Center, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

We introduce a new workflow that allows screening and selection of staged mammalian cells in mitosis prior to subsequent electron microscopy. We mainly describe four improved steps of specimen preparation. Firstly, we describe a method to efficiently enrich mammalian cells and attach them to sapphire discs; secondly, we report on the use of 3D-printed containers to seed cells on coated sapphire discs for high-pressure freezing; thirdly, we take advantage of a specimen carrier that allows for an upside-down placing of sapphire discs without a second carrier or spacer ring to close the "sandwich"; and fourthly, we use histological dyes to stain DNA/chromatin during freeze-substitution. Read More

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October 2020

Association of fall risk factors and non-motor symptoms in patients with early Parkinson's disease.

Sci Rep 2021 Mar 4;11(1):5171. Epub 2021 Mar 4.

Department of Neurology, Soonchunhyang University Seoul Hospital, Soonchunhyang University School of Medicine, 59 Daesagwan-ro, Yongsan-gu, Seoul, 04401, Republic of Korea.

The association of non-motor symptoms (NMSs) with fall-related factors in patients with Parkinson's disease (PD) remains to be further elucidated in the early stages of the disease. Eighty-six patients with less than 5 years of the onset of PD were retrospectively enrolled in the study. We assessed potential fall-related risk factors including (1) a history of falls during the past year (faller versus non-faller), (2) the fear of falling (FoF), and (3) the freezing of gait (FoG). Read More

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Cynodontium luthii sp. nov.: a permineralized moss gametophyte from the Late Cretaceous of the North Slope of Alaska.

Am J Bot 2021 03 2;108(3):495-504. Epub 2021 Mar 2.

Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, 97331, USA.

Premise: Mosses are a major component of Arctic vegetation today, with >500 species known to date. However, the origins of the Arctic moss flora are poorly documented in the fossil record, especially prior to the Pliocene. Here, we present the first anatomically preserved pre-Cenozoic Arctic moss and discuss how the unique biology of bryophytes has facilitated their success in polar environments over geologic time. Read More

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Chemogenetic inhibition of ventral hippocampal CaMKIIα-expressing neurons attenuates anxiety- but not fear-like defensive behaviors in male Long-Evans hooded rats.

Neurosci Lett 2021 04 26;751:135777. Epub 2021 Feb 26.

Department of Psychology, University of New Mexico, Albuquerque, NM, United States. Electronic address:

Previous research has implicated the ventral pole of the hippocampus in regulating anxiety. However, most rat studies examining the specific contribution of the ventral hippocampus have utilized techniques that have nonspecific effects and/or create nonreversible damage to the region. The present study sought to characterize the role of ventral hippocampal CaMKIIα-expressing neurons in modulating anxiety- and fear-like behavior during exposure to a variety of threatening stimuli. Read More

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