20,434 results match your criteria freezing methods


Identification and semi-quantification of Atlantic salmon in processed and mixed seafood products using Droplet Digital PCR (ddPCR).

Food Chem Toxicol 2021 Jun 8:112329. Epub 2021 Jun 8.

Flanders Research Institute for Agriculture, Fisheries and Food, ILVO, Aquatic Environment and Quality, Ankerstraat 1, B-8400, Oostende, Belgium; Department of Biology, Ghent University, Krijgslaan 281, Building S8, B-9000 Ghent, Belgium. Electronic address:

Fishery products are often subject to substitution fraud, which is hard to trace due to a lack of morphologic traits when processed, gutted, or decapitated. Traditional molecular methods (DNA barcoding) fail to identify products containing multiple species and cannot estimate original weight percentages. As a proof of concept, an Atlantic salmon (Salmo salar) specific ddPCR assay was designed to authenticate mixed food products. Read More

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[Effects of noise, bright light and mechanical stimulation on sleep, blood-brain barrier and cognitive function in septic rats].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 May;33(5):529-534

Department of Critical Care Medicine, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology (Xiaogan Central Hospital), Xiaogan 432000, Hubei, China. Corresponding author: Yao Peng, Email:

Objective: To evaluate the effects of noise, bright light and mechanical stimulation on sleep, blood-brain barrier and cognitive function in septic rats.

Methods: Forty male Sprague-Dawley (SD) rats were selected and intraperitoneal injection of 10 mg/kg lipopolysaccharide (LPS) was used to establish sepsis model. 0, 30, 45, 60, 75 dB noise stimulation or 0, 50, 100, 200, 400 Lux light stimulation were given to rats (all n = 4). Read More

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Medical recommender systems based on continuous-valued logic and multi-criteria decision operators, using interpretable neural networks.

BMC Med Inform Decis Mak 2021 Jun 11;21(1):186. Epub 2021 Jun 11.

Knowledgepark GmbH, Leonrodstr. 68, 80636, Munich, Germany.

Background: Out of the pressure of Digital Transformation, the major industrial domains are using advanced and efficient digital technologies to implement processes that are applied on a daily basis. Unfortunately, this still does not happen in the same way in the medical domain. For this reason, doctors usually do not have the time or knowledge to evaluate all alternative treatment options for each patient accurately and individually. Read More

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Truncating a densely connected convolutional neural network with partial layer freezing and feature fusion for diagnosing COVID-19 from chest X-rays.

MethodsX 2021 5;8:101408. Epub 2021 Jun 5.

Batangas State University.

Deep learning and computer vision revolutionized a new method to automate medical image diagnosis. However, to achieve reliable and state-of-the-art performance, vision-based models require high computing costs and robust datasets. Moreover, even with the conventional training methods, large vision-based models still involve lengthy epochs and costly disk consumptions that can entail difficulty during deployment due to the absence of high-end infrastructures. Read More

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Paired inhibitory stimulation and gait training modulates supplemental motor area connectivity in freezing of gait.

Parkinsonism Relat Disord 2021 Jun 1;88:28-33. Epub 2021 Jun 1.

Department of Neurology, Medical University of South Carlina, Charleston, SC, USA. Electronic address:

Introduction: Freezing of gait (FOG) is a debilitating feature of Parkinson's disease (PD). Evidence suggests patients with FOG have increased cortical control of gait. The supplementary motor area (SMA) may be a key structure due to its connectivity with locomotor and cognitive networks. Read More

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Red Blood Cell Cryopreservation with Minimal Post-Thaw Lysis Enabled by a Synergistic Combination of a Cryoprotecting Polyampholyte with DMSO/Trehalose.

Biomacromolecules 2021 Jun 7. Epub 2021 Jun 7.

Department of Chemistry, University of Warwick, Coventry CV4 7AL, U.K.

From trauma wards to chemotherapy, red blood cells are essential in modern medicine. Current methods to bank red blood cells typically use glycerol (40 wt %) as a cryoprotective agent. Although highly effective, the deglycerolization process, post-thaw, is time-consuming and results in some loss of red blood cells during the washing procedures. Read More

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Macroscopic-Scale Preparation of Aramid Nanofiber Aerogel by Modified Freezing-Drying Method.

ACS Nano 2021 Jun 4. Epub 2021 Jun 4.

Key Laboratory of Advanced Materials (MOE), Department of Chemical Engineering, Tsinghua University, No.1, Tsinghua Garden, Haidian District, Beijing 100084, P.R. China.

Aerogel has been widely known as a low-density and highly porous material and is closely connected with the complex processing methods, such as freeze-drying or supercritical drying. In this work, using the polymerization-induced aramid nanofiber (PANF) as a building block, we put forward a modified freezing-drying method for the high-efficiency preparation of all--aromatic-amide aerogels. In the preparation process, PANF hydrogel is first frozen at -18 °C and then dried at 20-150 °C for the formation of PANF aerogel. Read More

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An Audit and Comparison of pH, Measured Concentration, and Particulate Matter in Mannitol and Hypertonic Saline Solutions.

Front Neurol 2021 17;12:667842. Epub 2021 May 17.

Section of Neurosurgery, Department of Surgery, University of Manitoba, Health Sciences Centre, Winnipeg, MB, Canada.

The preferred hyperosmolar therapy remains controversial. Differences in physical properties such as pH and osmolality may be important considerations in hyperosmolar agent selection. We aimed to characterize important physical properties of commercially available hyperosmolar solutions. Read More

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Deep brain stimulation of the Cuneiform nucleus for levodopa-resistant freezing of gait in Parkinson's disease: study protocol for a prospective, pilot trial.

Pilot Feasibility Stud 2021 Jun 2;7(1):117. Epub 2021 Jun 2.

The Miami Project to Cure Paralysis, Miami, FL, USA.

Background: Freezing of gait (FOG) is a particularly debilitating motor deficit seen in a subset of Parkinson's disease (PD) patients that is poorly responsive to standard levodopa therapy or deep brain stimulation (DBS) of established PD targets such as the subthalamic nucleus and the globus pallidus interna. The proposal of a DBS target in the midbrain, known as the pedunculopontine nucleus (PPN) to address FOG, was based on its observed pathology in PD and its hypothesized involvement in locomotor control as a part of the mesencephalic locomotor region, a functionally defined area of the midbrain that elicits locomotion in both intact animals and decerebrate animal preparations with electrical stimulation. Initial reports of PPN DBS were met with much enthusiasm; however, subsequent studies produced mixed results, and recent meta-analysis results have been far less convincing than initially expected. Read More

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Effectiveness of water softening residuals as components of road deicing chemicals: Model analysis of freezing point depression.

J Environ Manage 2021 May 28;293:112888. Epub 2021 May 28.

Department of Civil Engineering, Kansas State University, Manhattan, KS, 66506, USA. Electronic address:

Water softening residuals disposal is a worldwide issue due to the lack of effective reuse alternatives. The current principal disposal methods of landfilling and land application are quite costly due to the dewatering and transportation costs involved, and these operations can also cause potential environmental harm from leaching of the additives used in the treatment process. This research is aimed at the use of water softening residuals in the production of biodegradable road deicers that would be beneficial in replacing the highly corrosive and environmentally harmful chloride salts that are currently used for road deicing. Read More

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Validating a Seated Virtual Reality Threat Paradigm for Inducing Anxiety and Freezing of Gait in Parkinson's Disease.

J Parkinsons Dis 2021 May 28. Epub 2021 May 28.

Parkinson's Disease Research Clinic, Brain and Mind Centre, University of Sydney, Australia.

Background: Although prior research has established that freezing of gait (FOG) in Parkinson's disease (PD) is associated with anxiety, only one study to date has directly manipulated anxiety levels to induce FOG.

Objective: The current study aimed to replicate these previous findings and evaluate whether a seated version of a 'threat' virtual reality (VR) paradigm could induce anxiety and provoke FOG.

Methods: Twenty-four PD patients with FOG were assessed across various threat conditions in both a walking VR paradigm (Experiment 1) and a seated VR paradigm (Experiment 2). Read More

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Short Pulse and Conventional Deep Brain Stimulation Equally Improve the Parkinsonian Gait Disorder.

J Parkinsons Dis 2021 May 28. Epub 2021 May 28.

University Medical Center Hamburg-Eppendorf, Department of Neurology, Hamburg, Germany.

Background: Gait disturbances and balance remain challenging issues in Parkinsonian patients (PD) with deep brain stimulation (DBS). Short pulse deep brain stimulation (spDBS) increases the therapeutic window in PD patients, yet the effect on gait and postural symptoms remains unknown.

Objective: We assessed the efficacy of spDBS compared to conventional DBS (cDBS) within the subthalamic nucleus (STN) on Parkinsonian gait. Read More

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Method for Accurate Quantitation of Volatile Organic Compounds in Urine Using Point of Collection Internal Standard Addition.

ACS Omega 2021 May 4;6(19):12684-12690. Epub 2021 May 4.

Tobacco and Volatiles Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway MS S103-3, Atlanta, Georgia 30341, United States.

A method to achieve accurate measurement of unmetabolized volatile organic compounds (VOCs) in urine was developed and characterized. The method incorporates a novel preanalytical approach of adding isotopically labeled internal standard (ISTD) analogues directly to the collection container at the point of collection to compensate for analyte loss to the headspace and the collection container surfaces. Using this approach, 45 toxic VOCs ranging in water solubility and boiling point were evaluated and analyzed by headspace solid-phase microextraction/gas chromatography-mass spectrometry. Read More

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Establishment of a novel cell-based assay using HLA-transfected cells to detect HLA antibodies.

J Immunol Methods 2021 May 26;495:113074. Epub 2021 May 26.

Japanese Red Cross Hokkaido Blood Center, Sapporo, Hokkaido, Japan.

The detection of HLA antibodies is important in clinical practice, such as platelet transfusion refractoriness and transfusion-related lung injury. However, difficulties are associated with the preparation of panel cells for conventional HLA detection systems using intact cells, such as the immunocomplex capture fluorescence analysis (ICFA). Based on an ICFA analysis, HEK293 cells stably transfected with the HLA-A locus were used instead of peripheral blood mononuclear cells (PBMC). Read More

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Effects of long-term cryopreservation of PBMC on recovery of B cell subpopulations.

J Immunol Methods 2021 May 26;495:113081. Epub 2021 May 26.

Division of Microbiology, Paul-Ehrlich-Institut, Langen, Germany. Electronic address:

Cryopreservation of human peripheral blood mononuclear cells (PBMC) is used in many clinical and research applications to avoid direct and on-site analysis of samples. Storage of PBMC further allows prequalification of donor cells for routine laboratory methods involving the evaluation of immune responses. Previous studies reported changes in cellular composition and phenotype of PBMC following the freezing procedure. Read More

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The impact of regular sperm donation on bulls' seminal plasma hormonal profile and phantom response.

Sci Rep 2021 May 27;11(1):11116. Epub 2021 May 27.

Malopolska Biotechnic Centre Ltd, Krasne, Poland.

The aim of this study was to analyze the relationship between the concentration of hormones in the seminal plasma, the bull maintenance system in the insemination station, and the regularity of sperm donation and the response to the phantom (libido level). An additional goal was to determine whether there is a relationship between the hormonal profile in the blood, the sperm plasma, the oxidative and antioxidant profile in the blood of bulls and the biometry of their testicles and scrotum, as well as the quality of their sperm in both different seasons and intensities of reproductive use. For the study, 220 healthy and sexually mature Polish Holstein-Friesian bulls were used. Read More

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Investigation into antibacterial and wound healing properties of platelets lysate against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections.

Ann Clin Microbiol Antimicrob 2021 May 27;20(1):40. Epub 2021 May 27.

Microbiology Department, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.

Background And Aim: Treatment of burn wound infections has become a global challenge due to the spread of multidrug-resistant bacteria; therefore, the development of new treatment options for the mentioned infections is essential. Platelets have drawn much attention for this purpose because they are a safe and cost-effective source of different antimicrobial peptides and growth factors. The present study evaluated antibacterial effects and wound healing properties of Platelet-derived Biomaterial (PdB) against Acinetobacter baumannii and Klebsiella pneumoniae burn wound infections. Read More

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Tetrandrine inhibits the occurrence and development of frozen shoulder by inhibiting inflammation, angiogenesis, and fibrosis.

Biomed Pharmacother 2021 May 24;140:111700. Epub 2021 May 24.

Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. Electronic address:

Background: Frozen shoulders (FS) is a major clinical concern, where chronic synovial inflammation, abnormal angiogenesis, and fibrosis represent the critical pathologies in the glenohumeral capsule. However, no pharmacotherapy has been introduced to treat this pathology. Tetrandrine (TET) has been proposed as a treatment for many diseases due to its strong anti-inflammatory, anti-angiogenic, and anti-fibrotic effects. Read More

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Intercalary reconstruction following resection of diaphyseal bone tumors: A systematic review.

J Clin Orthop Trauma 2021 Aug 7;19:1-10. Epub 2021 May 7.

Orthopaedic Service, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.

Introduction: The options for the reconstruction of diaphyseal defects following the resection of bone tumors include biological or prosthetic implants. The purpose of our study was to evaluate different types of intercalary reconstruction techniques, including massive bone allograft, extracorporeal devitalized autograft, vascularized free fibula, and modular prosthesis.

Methods: We performed a systematic review of articles using the terms diaphyseal bone tumor and intercalary reconstruction. Read More

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Rapid detection and enumeration of aerobic mesophiles in raw foods using dielectrophoresis.

J Microbiol Methods 2021 Jul 24;186:106251. Epub 2021 May 24.

Graduate School of Agricultural Science, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan. Electronic address:

The concept of dielectrophoresis (DEP), which involves the movement of neutral particles by induced polarization in nonuniform electric fields, has been exploited in various biological applications. However, only a few studies have investigated the use of DEP for detecting and enumerating microorganisms in foodstuffs. Therefore, we aimed to evaluate the accuracy and efficiency of a DEP-based method for enumerating viable bacteria in three raw foods: freshly cut lettuce, chicken breast, and minced pork. Read More

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How many gait initiation trials are necessary to reliably detect anticipatory postural adjustments and first step characteristics in healthy elderly and people with Parkinson's disease?

Gait Posture 2021 May 18;88:126-131. Epub 2021 May 18.

Department of Neurology, University Hospital Schleswig-Holstein, Christian-Albrechts-University, Kiel, Germany.

Background: The gait initiation (GI) process can be characterized by anticipatory postural adjustments (APAs) and first step characteristics. However, even within a constrained environment, it is unclear how many trials are necessary to obtain a reliable measurement of the GI process within one assessment.

Research Question: How many gait initiation trials are necessary to reliably detect APAs and first step characteristics in healthy elderly (HC) and people with Parkinson's disease with Freezing of Gait (PD + FOG) under single (ST) and dual task (DT) conditions and are there any potential systematic errors?

Methods: Thirty-eight PD + FOG (ON-medication) and 30 HC performed 5 trials of GI under ST and DT (auditory stroop test). Read More

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Cryopreservation and Preparation of Thawed Spermatozoa from Rhesus Macaques (Macaca mulatta) for In Vitro Fertilization.

J Am Assoc Lab Anim Sci 2021 May 23. Epub 2021 May 23.

Advances in assisted reproductive technologies in rhesus macaques have allowed the development of valuable models of human disease, particularly when combined with recent techniques for gene editing. While the ability to perform in vitro fertilization (IVF) in rhesus macaques is well established, this procedure has not yet been optimized. Specifically, damage to the sperm caused by cryopreservation (cryodamage) may lead to unsuccessful artificial insemination and low fertilization and blastocyst formation rates in vitro. Read More

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Influence of hydrogel encapsulation during cryopreservation of ovarian tissues and impact of post-thawing in vitro culture systems in a research animal model.

Clin Exp Reprod Med 2021 Jun 24;48(2):111-123. Epub 2021 May 24.

Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

Objective: Using domestic cats as a biomedical research model for fertility preservation, the present study aimed to characterize the influences of ovarian tissue encapsulation in biodegradable hydrogel matrix (fibrinogen/thrombin) on resilience to cryopreservation, and static versus non-static culture systems following ovarian tissue encapsulation and cryopreservation on follicle quality.

Methods: In experiment I, ovarian tissues (n=21 animals; 567 ovarian fragments) were assigned to controls or hydrogel encapsulation with 5 or 10 mg/mL fibrinogen (5 or 10 FG). Following cryopreservation (slow freezing or vitrification), follicle viability, morphology, density, and key protein phosphorylation were assessed. Read More

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Effects of Gocovri (Amantadine) Extended-Release Capsules on Motor Aspects of Experiences of Daily Living in People with Parkinson's Disease and Dyskinesia.

Neurol Ther 2021 May 22. Epub 2021 May 22.

Adamas Pharmaceuticals, Inc., Emeryville, CA, USA.

Introduction: Gocovri, a bedtime-administered delayed-release/extended-release capsule formulation of amantadine, is the only drug approved by the US Food and Drug Administration as levodopa-adjunctive therapy for the treatment of OFF episodes and/or dyskinesia in Parkinson's disease (PD). Part II of the Movement Disorder Society Unified Parkinson's Disease Rating Scale (MDS-UPDRS) assesses patient-perceived disability on experiences of daily living affected by PD motor symptoms. We analyzed Gocovri-related changes in MDS-UPDRS Part II ratings in two placebo-controlled clinical trials. Read More

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Vitrification yields higher cryo-survival rate than slow freezing in biopsied bovine in vitro produced blastocysts.

Theriogenology 2021 Sep 13;171:44-54. Epub 2021 May 13.

Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark. Electronic address:

Vitrification and slow freezing are the two commonly used embryo cryopreservation methods. In most studies, vitrification of intact embryos has proven superior in several respects, including cell and embryo survival and pregnancy rate. However, there is a lack of data for comparing these two methods in in vitro produced (IVP) bovine blastocysts, which have been subjected to the retrieval of trophectoderm (TE) biopsy. Read More

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September 2021

Disrupted functional brain network topology in Parkinson's disease patients with freezing of gait.

Neurosci Lett 2021 May 21;759:135970. Epub 2021 May 21.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address:

Background: Freezing of gait (FOG) is a common and debilitating gait disturbance in patients with Parkinson's disease (PD), but the potential mechanisms are still unclear. This study aimed to explore alterations in the topological organization of whole-brain functional networks in PD patients with FOG.

Methods: We recruited 75 patients with PD, 37 patients with FOG and 38 patients without FOG, to undergo resting-state functional magnetic resonance imaging (fMRI). Read More

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Standardization of electrolyte leakage data and a novel liquid nitrogen control improve measurements of cold hardiness in woody tissue.

Plant Methods 2021 May 22;17(1):53. Epub 2021 May 22.

Arnold Arboretum of Harvard University, 1300 Centre St., Boston, MA, 02131, USA.

Background: A variety of basic and applied research programs in plant biology require the accurate and reliable determination of plant tissue cold hardiness. Over the past 50 years, the electrolyte leakage method has emerged as a popular and practical method for quantifying the amount of damage inflicted on plant tissue by exposure to freezing temperatures. Numerous approaches for carrying out this method and analyzing the resultant data have emerged. Read More

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Pain quality of thermal grill illusion is similar to that of central neuropathic pain rather than peripheral neuropathic pain.

Scand J Pain 2021 May 21. Epub 2021 May 21.

Graduate School of Health Science, Kio University, Nara, Japan.

Objectives: Application of spatially interlaced innocuous warm and cool stimuli to the skin elicits illusory pain, known as the thermal grill illusion (TGI). This study aimed to discriminate the underlying mechanisms of central and peripheral neuropathic pain focusing on pain quality, which is considered to indicate the underlying mechanism(s) of pain. We compared pain qualities in central and peripheral neuropathic pain with reference to pain qualities of TGI-induced pain. Read More

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[Nutrients of fresh strawberries and products of its processing taking into account varietal characteristics].

Vopr Pitan 2021;90(2):117-127. Epub 2021 Mar 11.

North-Caucasian Federal Scientific Center for Horticulture, Viticulture, Winemaking, 350901, Krasnodar, Russian Federation.

In the context of a deficiency of some essential nutrients in the diet of the majority of Russian population, studies aimed at evaluation of the accumulation of nutrients in plant foods that determine the nutritional value and contribute to the improvement of the nutritional structure of the human diet are relevant. of this work was to evaluate content of macro- and micronutrients that determine nutritional value in strawberries, taking into account varietal characteristics, and to assess its opportunity to improve the structure of human nutrition when eaten fresh and in processed products. . Read More

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Short/long-term cryopreservation prior to comet assay of whole-blood leukocytes and -cultured lung fibroblasts.

Toxicol Mech Methods 2021 May 20:1-25. Epub 2021 May 20.

Institute of Biology, Ecology and Natural Resources, Kemerovo State University, Kemerovo, Russian Federation.

Single-cell gel electrophoresis (comet assay) is a valuable test that can be used in ecotoxicological, epidemiological, and biomonitoring contexts. We assessed the effects of short- (without cryopreservation) and long-term (with cryopreservation) storage of DMEM-cultivated human peripheral blood leukocytes (HPBLs) and a human lung fibroblast cell line (FLECH-104) on comet assay results. Samples were stored for 6 or 24 h at room temperature (23°С) or 4 °C and frozen at -80 °C or -196 °C for 1, 2, or 4 weeks. Read More

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