23 results match your criteria fragment mat1-1

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Polyphasic Discrimination of Trichophyton tonsurans and T. equinum from Humans and Horses.

Mycopathologia 2020 Feb 5;185(1):113-122. Epub 2019 Jul 5.

Centre of Expertise in Mycology, Radboud University Medical Centre/Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.

The anthropophilic dermatophyte Trichophyton tonsurans and its zoophilic counterpart T. equinum are phylogenetically closely related. The barcoding marker rDNA internal transcribed spacer (ITS) shows limited variation between these two species. Read More

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February 2020

Self-fertility in Chromocrea spinulosa is a consequence of direct repeat-mediated loss of MAT1-2, subsequent imbalance of nuclei differing in mating type, and recognition between unlike nuclei in a common cytoplasm.

PLoS Genet 2017 Sep 11;13(9):e1006981. Epub 2017 Sep 11.

Plant Pathology & Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, United States of America.

The filamentous fungus Chromocrea spinulosa (Trichoderma spinulosum) exhibits both self-fertile (homothallic) and self-sterile (heterothallic) sexual reproductive behavior. Self-fertile strains produce progeny cohorts that are 50% homothallic, 50% heterothallic. Heterothallic progeny can mate only with homothallic strains, and progeny also segregate 50% homothallic, 50% heterothallic. Read More

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September 2017

Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale.

Springerplus 2016 15;5(1):1085. Epub 2016 Jul 15.

CIRAD, UMR LSTM, 34398 Montpellier Cedex 5, France.

Background: This work aimed at characterizing 12 isolates of the genus Tuber including Tuber melanosporum (11 isolates) and Tuber brumale (one isolate). This was done using internal transcribed spacer (ITS) sequences, confirming their origin.

Results: Analysis of their mating type revealed that both MAT1-1 and MAT1-2 exist within these isolates (with 3 and 8 of each, respectively). Read More

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Discovery of a sexual cycle in Talaromyces amestolkiae.

Mycologia 2016 Jan-Feb;108(1):70-9. Epub 2015 Nov 17.

CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands

Talaromyces amestolkiae is a common cosmopolitan species that has been cultured from indoor house dust, sputum and lungs from cystic fibrosis patients, indoor air, wheat, soil, pineapple, sculptures and manure. It was described as an asexual Talaromyces species and was reported to produce black sclerotia. In this study we report on the induction of sexual reproductive structures in T. Read More

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Population Structure, Pathogenicity, and Mating Type Distribution of Magnaporthe oryzae Isolates from East Africa.

Phytopathology 2015 Aug 10;105(8):1137-45. Epub 2015 Aug 10.

First, third, and fifth authors: Division of Plant Pathology and Crop Protection, Department of Crop Sciences, and second author: CLB-Tropical and Subtropical Agriculture and Forestry, Georg-August-University Göttingen, Germany; and fourth author: Africa Rice Center, P.O. Box 33581, Dar es Salaam, Tanzania.

Rice blast, caused by Magnaporthe oryzae, is one of the emergent threats to rice production in East Africa (EA), where little is known about the population genetics and pathogenicity of this pathogen. We investigated the genetic diversity and mating type (MAT) distribution of 88 isolates of M. oryzae from EA and representative isolates from West Africa (WA) and the Philippines (Asia) using amplified fragment length polymorphism markers and mating-type-specific primer sets. Read More

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Genotypic and Phenotypic Characterization of Fungi in the Fusarium oxysporum Species Complex from Soybean Roots.

Phytopathology 2014 Dec;104(12):1329-39

Isolates in the Fusarium oxysporum species complex (FOSC) from soybean range from nonpathogenic to aggressive pathogens causing seedling damping-off, wilt, and root rot. The objective of this research was to characterize the genotype and phenotype of isolates within the FOSC recovered predominantly from soybean roots and seedlings. Sequence analyses of the translation elongation factor (tef1α) gene and the mitochondrial small subunit (mtSSU), polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis of the intergenic spacer (IGS) region, and identification of the mating type loci were conducted for 170 isolates. Read More

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December 2014

BOTH MAT1-1 AND MAT1-2 MATING TYPES OF MYCOSPHAERELLA GRAMINICOLA OCCUR AT EQUAL FREQUENCIES IN ALGERIA.

Commun Agric Appl Biol Sci 2014 ;79(3):469-72

Septoria tritici blotch caused by Mycosphaerella graminicola is currently the most devastating disease on wheat crops worldwide. Mycosphaerella graminicola sexual reproduction involves two mating type idiomorphs that were previously studied in several areas around the world, but not in Algeria so far. The objective of this study was thus to determine the frequencies and distribution of M. Read More

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The heterothallic sugarbeet pathogen Cercospora beticola contains exon fragments of both MAT genes that are homogenized by concerted evolution.

Fungal Genet Biol 2014 Jan 8;62:43-54. Epub 2013 Nov 8.

Department of Plant Pathology, North Dakota State University, Fargo, ND, United States.

Dothideomycetes is one of the most ecologically diverse and economically important classes of fungi. Sexual reproduction in this group is governed by mating type (MAT) genes at the MAT1 locus. Self-sterile (heterothallic) species contain one of two genes at MAT1 (MAT1-1-1 or MAT1-2-1) and only isolates of opposite mating type are sexually compatible. Read More

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January 2014

Identification of the mating-type (MAT) locus that controls sexual reproduction of Blastomyces dermatitidis.

Eukaryot Cell 2013 Jan 9;12(1):109-17. Epub 2012 Nov 9.

Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA.

Blastomyces dermatitidis is a dimorphic fungal pathogen that primarily causes blastomycosis in the midwestern and northern United States and Canada. While the genes controlling sexual development have been known for a long time, the genes controlling sexual reproduction of B. dermatitidis (teleomorph, Ajellomyces dermatitidis) are unknown. Read More

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January 2013

Phylogeography of the cosmopolitan fungus Aspergillus flavus: is everything everywhere?

Fungal Biol 2012 Mar 28;116(3):452-63. Epub 2012 Jan 28.

Department of Biology, University of Puerto Rico - Río Piedras, San Juan 00931, Puerto Rico.

Aspergillus flavus is one of the most common fungal eukaryotes on the planet. It is notorious for production of aflatoxins, for causing aspergillosis in humans and animals, and as an opportunistic pathogen of animals and plants. Its role in marine habitats is unclear. Read More

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Magnaporthe oryzae populations adapted to finger millet and rice exhibit distinctive patterns of genetic diversity, sexuality and host interaction.

Mol Biotechnol 2012 Feb;50(2):145-58

University of Warwick, Coventry, UK.

In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of clonal lineages, with a wide range of haplotypes. M. Read More

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February 2012

Single strand conformation polymorphism is a sensitive method for screening nucleotide variations in Mycosphaerella graminicola.

Commun Agric Appl Biol Sci 2010 ;75(4):593-9

Univ. Ulle-Nord de France.

Single Strand Conformation Polymorphism (SSCP) and sequencing were performed in order to assess molecular polymorphism of mating type sequences in the heterothallic ascomycete Mycosphaerella graminicola, the causal agent of Septoria tritici blotch of wheat. The screening was undertaken on mat1-1 and mat1-2 partial sequences of 341 and 657 bp, respectively, amplified with multiplex PCR from 510 French single-conidial strains plus the two reference isolates IPO323 and IPO94269 from The Netherlands. After restriction with Taq1 in order to reduce the fragment sizes, all digested amplicons were subjected to SSCP. Read More

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Phylogeny and evolution of mating-type genes from Pyrenophora teres, the causal agent of barley "net blotch" disease.

Curr Genet 2007 Jun 11;51(6):377-92. Epub 2007 Apr 11.

Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.

The main aim of this study was to test the patterns of sequence divergence and haplotype structure at the MAT locus of Pyrenophora teres, the causal agent of barley 'net blotch' disease. P. teres is a heterothallic ascomycete that co-occurs in two symptomatological forms, the net form (NF) and the spot form (SF). Read More

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Isolation and characterization of the mating type locus of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana.

Mol Plant Pathol 2007 Jan;8(1):111-20

Centro de Investigación Científica de Yucatán (CICY), Calle 43 no. 130, Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, México.

SUMMARY Idiomorphs mat1-1 and mat1-2 from Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana, were isolated. Degenerate oligos were used to amplify the HMG box of the mat1-2 idiomorph from M. fijiensis, showing homology with the HMG box of Mycosphaerella graminicola. Read More

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January 2007

Mating-type genes and the genetic structure of a world-wide collection of the tomato pathogen Cladosporium fulvum.

Fungal Genet Biol 2007 May 18;44(5):415-29. Epub 2006 Dec 18.

Laboratory of Phytopathology, Wageningen University and Research Centre, Binnenhaven 5, 6709 PD Wageningen, The Netherlands.

Two mating-type genes, designated MAT1-1-1 and MAT1-2-1, were cloned and sequenced from the presumed asexual ascomycete Cladosporium fulvum (syn. Passalora fulva). The encoded products are highly homologous to mating-type proteins from members of the Mycosphaerellaceae, such as Mycosphaerella graminicola and Cercospora beticola. Read More

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Significant difference in pathogenicity between MAT1-1 and MAT1-2 isolates in the wheat pathogen Mycosphaerella graminicola.

Fungal Genet Biol 2007 May 8;44(5):339-46. Epub 2006 Dec 8.

Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK.

Five Mycosphaerella graminicola populations from four geographic regions (Australia, Israel, Switzerland, and the USA) were assayed for neutral RFLP markers and mating type idiomorphs. On average, 25-30 genetically distinct isolates were selected from each population and their pathogenicity was measured on two wheat cultivars in a common garden experiment conducted in a greenhouse. A significant difference in pathogenicity was found between MAT1-1 and MAT1-2 isolates. Read More

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Cloning of the mating type locus from Ascochyta lentis (teleomorph: Didymella lentis) and development of a multiplex PCR mating assay for Ascochyta species.

Curr Genet 2006 Sep 18;50(3):203-15. Epub 2006 Jul 18.

Laboratoire de Phytopathologie, Institut National Agronomique de Tunisie, Cité Mahrajéne, Tunis, Tunisia.

The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers were designed to specifically amplify MAT from several Ascochyta spp. Read More

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September 2006

Characterisation of the mating-type locus in the genus Xanthoria (lichen-forming ascomycetes, Lecanoromycetes).

Fungal Genet Biol 2005 Dec 2;42(12):976-88. Epub 2005 Nov 2.

Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8008 Zurich, Switzerland.

Conserved regions of mating-type genes were amplified in four representatives of the genus Xanthoria (X. parietina, X. polycarpa, X. Read More

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December 2005

Evidence for sexuality in the opportunistic fungal pathogen Aspergillus fumigatus.

Curr Biol 2005 Jul;15(13):1242-8

School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom.

Aspergillus fumigatus is a medically important opportunistic pathogen and a major cause of respiratory allergy. The species has long been considered an asexual organism. However, genome analysis has revealed the presence of genes associated with sexual reproduction, including a MAT-2 high-mobility group mating-type gene and genes for pheromone production and detection (Galagan et al. Read More

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Historical and contemporary multilocus population structure of Ascochyta rabiei (teleomorph: Didymella rabiei) in the Pacific Northwest of the United States.

Mol Ecol 2004 Feb;13(2):291-309

Department of Plant Pathology and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USA.

The historical and contemporary population genetic structure of the chickpea Ascochyta blight pathogen, Ascochyta rabiei (teleomorph: Didymella rabiei), was determined in the US Pacific Northwest (PNW) using 17 putative AFLP loci, four genetically characterized, sequence-tagged microsatellite loci (STMS) and the mating type locus (MAT). A single multilocus genotype of A. rabiei (MAT1-1) was detected in 1983, which represented the first recorded appearance of Ascochyta blight of chickpea in the PNW. Read More

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February 2004

Sequence diversity of mating-type genes in Phaeosphaeria avenaria.

Curr Genet 2003 May 4;43(2):121-30. Epub 2003 Mar 4.

Molecular Plant Pathology Laboratory, U.S. Department of Agriculture, ARS, Beltsville, MD 20705, USA.

Phaeosphaeria avenaria, one of the causal agents of stagonospora leaf blotch diseases in cereals, is composed of two subspecies, P. avenaria f. sp. Read More

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Multilocus population structure of Tapesia yallundae in Washington State.

Mol Ecol 2002 Nov;11(11):2229-39

Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA.

Population genetic structure of the fungal wheat pathogen Tapesia yallundae in Washington State was determined using genetically characterized amplified fragment length polymorphic (AFLP) markers and mating-type (MAT1-1 or MAT1-2). Segregation and linkage relationships among 164 AFLP markers and MAT were analysed using 59 progeny derived from an in vitro cross. Alleles at 158 AFLP loci and the mating-type locus segregated in a 1:1 ratio. Read More

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November 2002

Cross-Compatibility and Distribution of Mating Type Alleles of the Rice Blast Fungus Magnaporthe grisea in India.

Plant Dis 2000 Jun;84(6):700-704

Professor, Center for Advanced Studies in Botany, University of Madras, Chennai 600025, India.

Two hundred twenty-seven isolates of Magnaporthe grisea isolated from blast-infected rice tissues from different states of India were tested with MAT1-1 and MAT1-2 fertile standard testers to determine their mating type. Of the 227 monoconidial isolates, 90 (39.6%) were fertile and 137 (60. Read More

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