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Chemical profiling, in vitro antioxidant, membrane stabilizing and antimicrobial properties of wild growing Murraya paniculata from Amarkantak (M.P.).

Sci Rep 2021 May 7;11(1):9691. Epub 2021 May 7.

Department of Biotechnology, Indira Gandhi National Tribal University, Amarkantak, Anuppur, Madhya Pradesh, 484887, India.

The excessive usage of antibiotics in humans and veterinary medicine has lead to the emergence of antibiotic resistance and now requires the use of novel antibiotics. There has been increased interest towards plants as source of drugs because of their pharmacological potency and long traditional usage. The aim of the current study was to evaluate bioactive components, antioxidant, and anti-inflammatory activities of the leaf extracts of Murraya paniculata, a plant traditionally used in Indian medicinal system. Read More

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Counteracting the Ramifications of UVB Irradiation and Photoaging with King Seed.

Molecules 2021 Apr 1;26(7). Epub 2021 Apr 1.

Biofunctional Molecule Exploratory Research Group, School of Pharmacy, Monash University Malaysia, Bandar Sunway 47500, Malaysia.

In this day and age, the expectation of cosmetic products to effectively slow down skin photoaging is constantly increasing. However, the detrimental effects of UVB on the skin are not easy to tackle as UVB dysregulates a wide range of molecular changes on the cellular level. In our research, irradiated keratinocyte cells not only experienced a compromise in their redox system, but processes from RNA translation to protein synthesis and folding were also affected. Read More

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Metallobiochemistry of ultratrace levels of bismuth in the rat II. Interaction of Bi with tissue, intracellular and molecular components.

J Trace Elem Med Biol 2021 Apr 20:126752. Epub 2021 Apr 20.

Department of Physics, Università Degli Studi di Milano, Via Celoria 16, Milano, I-20133, Italy; LASA, Department of Physics, Università Degli Studi di Milano and INFN-Milano, Via F.lli Cervi 201, Segrate, MI, I-20090, Italy. Electronic address:

Background: Knowledge on Bi metabolism in laboratory animals refers to studies at "extreme" exposures, i.e. pharmacologically relevant high-doses (mg kg b. Read More

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Seasonal succession of microbes in different size-fractions and their modular structures determined by both macro- and micro-environmental filtering in dynamic coastal waters.

Sci Total Environ 2021 Apr 20;784:147046. Epub 2021 Apr 20.

Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Department of Environmental Biotechnology, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. Electronic address:

Microbes interact with each other in response to various environmental changes in coastal marine ecosystems. To explore how the macroenvironment (environmental filtering) and species-engineered microenvironment (niche construction) affect the ecological network of the marine microbiome in the highly dynamic coastal waters of Korea, we analyzed the modular structures of the microbial community and identified microbial interconnections in different size fractions for a year. Fluctuations in the macroenvironment, such as temperature and nutrient concentrations driven by seasonal changes, are the major factors in determining successive microbial modules. Read More

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Individual and joint effects of phthalate metabolites on biomarkers of oxidative stress among pregnant women in Puerto Rico.

Environ Int 2021 Apr 23;154:106565. Epub 2021 Apr 23.

Department of Environmental Health Sciences, University of Michigan School of Public Health, Ann Arbor, MI, USA. Electronic address:

Exposures to phthalate compounds have been linked to adverse birth outcomes, potentially through oxidative stress mechanisms. We explored associations between mixtures of biomarkers of phthalate and phthalate replacement metabolites and oxidative stress using lipid peroxidation biomarker 8-iso-prostaglandin-F2α (8-iso-PGF2α). As 8-iso-PGF2α can be generated via both chemical (nonenzymatic) and enzymatic lipid peroxidation pathways, we calculated the ratio of 8-iso-PGF2α/prostaglandin F2α in an attempt to distinguish the potential contributions of the two pathways. Read More

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Genetics of HLA peptide presentation, and impact on outcomes in HLA-matched allo-HCT.

Transplant Cell Ther 2021 Apr 18. Epub 2021 Apr 18.

Internal Medicine, University of North Carolina, Chapel Hill, NC, USA. Electronic address:

Background: Minor histocompatibility antigens (mHA), which are recipient derived peptide epitopes presented on the cell surface, are known to mediate GVHD; however, there are no current methods to associate mHA features with GVHD risk. This deficiency is due in part to the lack of technological means to accurately predict, let alone confirm, the tremendous number of potential mHA in each individual transplant. Prior studies have shown that different HLA molecules present varying fractions of candidate peptide epitopes; however, the genetic "distance" between HLA matched donors and recipients is relatively constrained. Read More

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Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.

Int J Biol Macromol 2021 Apr 6;182:187-196. Epub 2021 Apr 6.

Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018, Shandong, PR China. Electronic address:

In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L. A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L. They differed in monosaccharide composition, molecular weight and linkage mode. Read More

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Metaproteomic sample preparation methods bias the recovery of host and microbial proteins according to taxa and cellular compartment.

J Proteomics 2021 May 5;240:104219. Epub 2021 Apr 5.

The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD, Australia; Correspondence should be addressed to A/Prof Emma Hamilton-Williams, The University of Queensland Diamantina Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia. Electronic address:

Faecal proteomics studies have focussed on identification of microbial proteins, however; stool represents a valuable resource to interrogate the host interactions with the microbiota without the need for invasive intestinal biopsies. As the widely used enrichment method (differential centrifugation, DC) enriches for microbial proteins, we compared two other methods for enrichment of host proteins, termed 'host enriched' (HE) and ALL (all proteins). The HE and ALL protocols identified 1. Read More

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Unravelling the anthelmintic bioactives from Jasminum grandiflorum L. subsp. Floribundum adopting in vitro biological assessment.

J Ethnopharmacol 2021 Apr 6;275:114083. Epub 2021 Apr 6.

Departement of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, P.O. Box 12211, Egypt. Electronic address:

Ethnopharmacological Relevance: Jasminum grandiflorum L. is a medicinal plant widely used in the traditional system of Medicine as an anthelmintic in ringworm infections, for treating ulcers, stomatitis, skin diseases, and wounds.

Aim Of The Study: The emergence of resistance by different parasites to currently used chemicals has been reported. Read More

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Characterizing the cytotoxic effects and several antimicrobial phytocompounds of Argemone mexicana.

PLoS One 2021 7;16(4):e0249704. Epub 2021 Apr 7.

Department of Biology, Valparaiso University, Valparaiso, IN, United States of America.

Commonly called the Mexican prickly poppy, Argemone mexicana is a stress-resistant member of the Papaveraceae family of plants that has been used in traditional medicine for centuries by indigenous communities in Mexico and Western parts of the United States. This plant has been exploited to treat a wide variety of ailments, with reported antimicrobial and antioxidant properties, as well as cytotoxic effects against some human cancer cell lines. Due to its various therapeutic uses and its abundance of secondary metabolites, A. Read More

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Subcellular Localization of Acyl-CoA: Lysophosphatidylethanolamine Acyltransferases (LPEATs) and the Effects of Knocking-Out and Overexpression of Their Genes on Autophagy Markers Level and Life Span of .

Int J Mol Sci 2021 Mar 16;22(6). Epub 2021 Mar 16.

Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, 80-307 Gdansk, Poland.

possesses two acyl-CoA:lysophosphatidylethanolamine acyltransferases, LPEAT1 and LPEAT2, which are encoded by and genes, respectively. Both single mutant and double mutant plants exhibit a variety of conspicuous phenotypes, including dwarfed growth. Confocal microscopic analysis of tobacco suspension-cultured cells transiently transformed with green fluorescent protein-tagged versions of LPEAT1 or LPEAT2 revealed that LPEAT1 is localized to the endoplasmic reticulum (ER), whereas LPEAT2 is localized to both Golgi and late endosomes. Read More

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Biodiscovery of Potential Antibacterial Diagnostic Metabolites from the Endolichenic Fungus Using LC-MS-Based Metabolomics.

Biology (Basel) 2021 Mar 4;10(3). Epub 2021 Mar 4.

School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Dahrul Ehsan 47500, Malaysia.

Three species of the lichen ( (Stirt.) Zahlbr., Zahlbr. Read More

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In Vitro Anticancer Potential of and Its Main Components against Human Amelanotic Melanoma Cells.

Int J Mol Sci 2021 Mar 25;22(7). Epub 2021 Mar 25.

Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland.

L. (Campanulaceae) is used in traditional Belarusian herbal medicine for sleep disorders in children, but the chemical composition and biological activity have not been investigated. In this study, the activities of extracts, their fractions and main compounds were evaluated in amelanotic melanoma C32 (CRL-1585) cells and normal fibroblasts (PCS-201-012). Read More

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Antioxidant and Antiproliferative Potentials of and Its Bioactive Polyphenol Metabolites.

Pharmaceuticals (Basel) 2021 Mar 15;14(3). Epub 2021 Mar 15.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.

Delile (Moraceae), a reputed plant that is used in herbal medicine, is of high medicinal and nutritional value in local communities primarily ascribed to its phytochemical profile. Currently, there are hardly any fine details on the chemical profiling and pharmacological evaluation of this species. In this study, the flavonoids and phenolics contents of the ethanol extracts and four extracted fractions (petroleum ether (PE), ethyl acetate (EA), -butanol, and water) of the stem bark of were firstly quantified. Read More

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A Metabolomics and Molecular Networking Approach to Elucidate the Structures of Secondary Metabolites Produced by Strains.

Front Chem 2021 16;9:633870. Epub 2021 Mar 16.

Department of Microbiology, Faculty of Science, Stellenbosch University, Stellenbosch, South Africa.

An integrated approach that combines reverse-phase high-performance liquid chromatography (RP-HPLC), electrospray ionization mass spectrometry, untargeted ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS) and molecular networking (using the Global Natural Products Social molecular network platform) was used to elucidate the metabolic profiles and chemical structures of the secondary metabolites produced by pigmented (P1) and non-pigmented (NP1) () strains. Tandem mass spectrometry-based molecular networking guided the structural elucidation of 18 compounds for the P1 strain (including 6 serratamolides, 10 glucosamine derivatives, prodigiosin and serratiochelin A) and 15 compounds for the NP1 strain (including 8 serratamolides, 6 glucosamine derivatives and serratiochelin A) using the MS fragmentation profiles. The serratamolide homologues were comprised of a peptide moiety of two L-serine residues (cyclic or open-ring) linked to two fatty acid chains (lengths of C, C, or C). Read More

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Comparison of free and bound phenolic compositions and antioxidant activities of leaves from different mulberry varieties.

BMC Chem 2021 Mar 29;15(1):21. Epub 2021 Mar 29.

Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

Mulberry leaves are used in traditional Chinese medicine and contain numerous active substances that are known to be beneficial for human health. The aim of this study was to investigate the phenolic compositions and antioxidant activities of the leaves from 23 mulberry cultivars. Qualitative LC-ESI-QTOF analysis revealed the presence of 11 phenolic compounds in the free phenolic extracts and 10 phenolic compounds in the bound fractions. Read More

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Preoperative immune landscape predisposes adverse outcomes in hepatocellular carcinoma patients with liver transplantation.

NPJ Precis Oncol 2021 Mar 26;5(1):27. Epub 2021 Mar 26.

Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, Republic of Korea.

Immune class in hepatocellular carcinoma (HCC) has been shown to possess immunogenic power; however, how preestablished immune landscapes in premalignant and early HCC stages impact the clinical outcomes of HCC patients remains unexplored. We sequenced bulk transcriptomes for 62 malignant tumor samples from a Korean HCC cohort in which 38 patients underwent total hepatectomy, as well as for 15 normal and 47 adjacent nontumor samples. Using in silico deconvolution of expression mixtures, 22 immune cell fractions for each sample were inferred, and validated with immune cell counting by immunohistochemistry. Read More

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SARS-CoV-2 infection of the oral cavity and saliva.

Nat Med 2021 Mar 25. Epub 2021 Mar 25.

Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Despite signs of infection-including taste loss, dry mouth and mucosal lesions such as ulcerations, enanthema and macules-the involvement of the oral cavity in coronavirus disease 2019 (COVID-19) is poorly understood. To address this, we generated and analyzed two single-cell RNA sequencing datasets of the human minor salivary glands and gingiva (9 samples, 13,824 cells), identifying 50 cell clusters. Using integrated cell normalization and annotation, we classified 34 unique cell subpopulations between glands and gingiva. Read More

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Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from Ziziphus jujuba cv. Hamidazao: A comparison.

Carbohydr Polym 2021 Jun 1;261:117879. Epub 2021 Mar 1.

College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an, 271018, Shandong, PR China. Electronic address:

A neutral polysaccharide (HJP-1a) and three acid polysaccharides (HJP-2, HJP-3 and HJP-4) were obtained from Z. jujuba cv. Hamidazao. Read More

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Pre-existing effector T-cell levels and augmented myeloid cell composition denote response to CDK4/6 inhibitor palbociclib and pembrolizumab in hormone receptor-positive metastatic breast cancer.

J Immunother Cancer 2021 Mar;9(3)

Medical Oncology, City of Hope National Medical Center, Duarte, California, USA

Background: Single-agent pembrolizumab treatment of hormone receptor-positive metastatic breast cancer (MBC) has demonstrated modest clinical responses. Little is known about potential biomarkers or mechanisms of response to immune checkpoint inhibitors (ICIs) in patients with HR+ MBC. The present study presents novel immune correlates of clinical responses to combined treatment with CDK4/6i and ICI. Read More

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Blood pressure and sugar regulating potentials of nut globulin and albumin hydrolysates.

Heliyon 2021 Mar 12;7(3):e06384. Epub 2021 Mar 12.

Department of Biological Sciences, College of Science and Engineering, Landmark University, Omu-Aran, Nigeria.

Several novel functional peptides have been successfully extracted from plant storage proteins. This study investigated the degree of hydrolysis, peptide yield, amino acid constituents, angiotensin converting enzyme (ACE), alpha amylase inhibitory and antioxidant activities of cashew () nut proteins (CNP) hydrolysates (CNPHs). Cashew nut proteins (albumin and globulin) were hydrolysed using pancreatin, Alcalase and trypsin. Read More

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Untangling the response of fungal community structure, composition and function in soil aggregate fractions to food waste compost addition.

Sci Total Environ 2021 May 20;769:145248. Epub 2021 Jan 20.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; State Environmental Protection Key Laboratory of Simulation and Control of Groundwater Pollution, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address:

Soil fungi are key drivers in regulating the ecosystem function, playing a vital role in protecting the plant from phytopathogens and other biotic and abiotic pressures. However, the potential impact of compost addition and soil aggregate size on the fungal community and functional ecological guild remains uncertain. This study investigated the structure, composition, and function of soil fungal communities across aggregate fractions under food waste compost addition using Miseq sequencing and FUNGuild. Read More

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Bioactive compounds from Octopus vulgaris ink extracts exerted anti-proliferative and anti-inflammatory effects in vitro.

Food Chem Toxicol 2021 May 17;151:112119. Epub 2021 Mar 17.

Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, 1658, Hermosillo, Sonora, Mexico. Electronic address:

Underutilized marine food products such as cephalopods' ink could be sources of bioactive compounds providing health benefits. This study aimed to assess the anti-proliferative and anti-inflammatory effects from Octopus vulgaris ink extracts (hexane-, ethyl acetate-, dichloromethane- (DM), and water extracts) using human colorectal (HT-29/HCT116) and breast (MDA-MB-231) cancer cells, and LPS-challenged murine RAW 264.7 cells. Read More

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Preliminary results of simultaneous integrated boost intensity-modulated radiation therapy based neoadjuvant chemoradiotherapy on locally advanced rectal cancer with clinically suspected positive lateral pelvic lymph nodes.

Ann Transl Med 2021 Feb;9(3):217

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing, China.

Background: Lateral pelvic lymph node (LPLN) is approximately 11-14% and always associated with poorer prognosis. This study investigated the efficacy and safety of simultaneous integrated boost intensity-modulated radiation therapy (SIB-IMRT) based on neoadjuvant chemoradiotherapy (NCRT) on locally advanced rectal cancer (LARC) patients with clinically suspected positive LPLNs.

Methods: We retrospectively screened distal LARC patients with NCRT in our center from May 2016 and June 2019. Read More

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February 2021

Exploration of microbiome of medicinally important plants as biocontrol agents against Phytophthora parasitica.

Arch Microbiol 2021 Mar 6. Epub 2021 Mar 6.

Department of Plant Pathology, Mid-Florida Research and Education Center, University of Florida/Institute of Food and Agricultural Sciences, 2725 Binion Rd, Apopka, FL, 32703, USA.

In a preliminary plant-based microbiome study, diverse bacterial taxa were identified from different medicinal plants using 16S rRNA gene sequencing. Based on initial antimicrobial screening, eight (8) bacterial endophytes in six (6) different genera, Streptomyces, Pseudomonas, Enterobacter, Bacillus, Arthrobacter, and Delftia, from four important medicinal plants Dodonaea viscosa, Fagonia indica, Caralluma tuberculata, and Calendula arvensis were selected for further analyses. Antimicrobial assays revealed that Pseudomonas taiwanensis MOSEL-RD23 has strong anti-Phytophthora activity. Read More

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Circulating Inhibitory Factor 1 levels in adult patients with Prader-Willi syndrome.

Horm Mol Biol Clin Investig 2021 Mar 5. Epub 2021 Mar 5.

Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari "Aldo Moro", Bari, Italy.

Objectives: Prader-Willi syndrome (PWS) is a rare genetic syndrome characterized by hyperphagia and early development of morbid obesity. Cardiovascular disease (CVD) and metabolic syndrome (MetS) are major comorbidities in these patients leading to premature death. Inhibitory factor 1 (IF) works as a regulatory protein, inhibiting the ATP hydrolase activity of mitochondrial ATP synthase and likely playing a role in lipid metabolism. Read More

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The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells.

Molecules 2021 Feb 22;26(4). Epub 2021 Feb 22.

School of Food and Advanced Technology, Massey University, Palmerston North 4442, New Zealand.

Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases. The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells. Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards. Read More

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February 2021

Fructose Induces Fluconazole Resistance in through Activation of Mdr1 and Cdr1 Transporters.

Int J Mol Sci 2021 Feb 21;22(4). Epub 2021 Feb 21.

Department of Biotransformation, Faculty of Biotechnology, University of Wrocław, 50-383 Wrocław, Poland.

is a pathogenic fungus that is increasingly developing multidrug resistance (MDR), including resistance to azole drugs such as fluconazole (FLC). This is partially a result of the increased synthesis of membrane efflux transporters Cdr1p, Cdr2p, and Mdr1p. Although all these proteins can export FLC, only Cdr1p is expressed constitutively. Read More

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February 2021

Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from (Vahl) Mast. Growing in Tunisia.

Molecules 2021 Feb 19;26(4). Epub 2021 Feb 19.

Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Medicinal Chemistry and Natural Products Team, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia.

The aim of the present research was to determine the chemical composition and the cytotoxic effects of trunk bark essential oil (HEE) obtained by steam distillation and five fractions obtained by normal phase silica chromatographic separation. Chemical analysis allowed the identification of 54 known compounds. Relatively high amounts of oxygenated sesquiterpenes (44. Read More

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February 2021

Feasibility of off-pump coronary artery grafting for patients with impaired left ventricular ejection fraction: A retrospective cohort study from a single institutional database.

J Card Surg 2021 Jun 4;36(6):1935-1942. Epub 2021 Mar 4.

Department of Cardiothoracic Surgery, Huashan Hospital, Fudan University, Shanghai, PR China.

Objective: Despite substantial advances in surgical practice, the management of patients with impaired left ventricular ejection fraction (LVEF) remains challenging. Furthermore, evidence on the outcomes of off-pump coronary artery bypass (OPCAB) surgery in this population is inconsistent. We conducted the present study to compare the short- and long-term outcomes of OPCAB in patients with different ejection fractions. Read More

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