J Proteomics 2021 Apr 5:104219. Epub 2021 Apr 5.
The University of Queensland Diamantina Institute, The University of Queensland, Woolloongabba, QLD, Australia; Correspondence should be addressed to A/Prof Emma Hamilton-Williams, The University of Queensland Diamantina Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia. Electronic address:
Faecal proteomics studies have focussed on identification of microbial proteins, however; stool represents a valuable resource to interrogate the host interactions with the microbiota without the need for invasive intestinal biopsies. As the widely used enrichment method (differential centrifugation, DC) enriches for microbial proteins, we compared two other methods for enrichment of host proteins, termed 'host enriched' (HE) and ALL (all proteins). The HE and ALL protocols identified 1. Read More