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Single-dose immunisation with a multimerised SARS-CoV-2 receptor binding domain (RBD) induces an enhanced and protective response in mice.

FEBS Lett 2021 Jul 31. Epub 2021 Jul 31.

MRC Laboratory of Molecular Biology, Cambridge, UK.

The COVID-19 pandemic, caused by the SARS-CoV-2 coronavirus, has triggered a worldwide health emergency. Here, we show that ferritin-like Dps from hyperthermophilic Sulfolobus islandicus, covalently coupled with SARS-CoV-2 antigens via the SpyCatcher system, forms stable multivalent dodecameric vaccine nanoparticles that remain intact even after lyophilisation. Immunisation experiments in mice demonstrated that the SARS-CoV-2 receptor binding domain (RBD) coupled to Dps (RBD-S-Dps) elicited a higher antibody titre and an enhanced neutralising antibody response compared to monomeric RBD. Read More

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Effect of EPS and its forms of aerobic granular sludge on sludge aggregation performance during granulation process based on XDLVO theory.

Sci Total Environ 2021 Jun 25;795:148682. Epub 2021 Jun 25.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China; School of Environment and Ecology, Chongqing University, Chongqing 400045, China.

Different forms of extracellular polymeric substances (EPS) play different roles in the formation process of aerobic granular sludge (AGS). This work focused on the contribution of loosely bound EPS (LB-EPS), tightly bound EPS (TB-EPS) and EPS to the aggregation between sludge cells during the start-up of aerobic granular sludge in a sequencing batch reactor. By analyzing the changes of sludge surface characteristics before and after the extraction of each layer of EPS, the contribution of LB-EPS, TB-EPS and EPS to the adhesion and aggregation of sludge cells in the granulation was calculated by surface thermodynamics and the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory. Read More

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Manganese-mediated hydrochemistry and microbiology in a meromictic subalpine lake (Lake Idro, Northern Italy) - A biogeochemical approach.

Sci Total Environ 2021 Jul 1;795:148743. Epub 2021 Jul 1.

Ricerca sul Sistema Energetico, RSE S.p.A., Department of Sustainable Development and Energy Sources, Via Rubattino 54, 20134 Milano, Italy.

This study presents the findings from several field campaigns carried out in Lake Idro (Northern Italy), a deep (124 m) meromictic-subalpine lake, whose water column is subdivided in a mixolimnion (~0-40 m) and a monimolimnion (~40-124 m). Hydrochemical data highlight two main peculiarities characterizing the Lake Idro meromixis: a) presence of a high manganese/iron ratio (up to 20 mol/mol), b) absence of a clear chemocline between the two main layers. The high manganese content contributed to the formation of a stable manganese dominated deep turbid stratum (40-65 m), enveloping the redoxcline (~45-55 m) in the upper monimolimnion. Read More

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Resonating neurons stabilize heterogeneous grid-cell networks.

Elife 2021 Jul 30;10. Epub 2021 Jul 30.

Molecular Biophysics Unit, Indian Institute of Science, Bangalore, Bangalore, India.

A central theme that governs the functional design of biological networks is their ability to sustain stable function despite widespread parametric variability. Here, we investigated the impact of distinct forms of biological heterogeneities on the stability of a two-dimensional continuous attractor network (CAN) implicated in grid-patterned activity generation. We show that increasing degrees of biological heterogeneities progressively disrupted the emergence of grid-patterned activity and resulted in progressively large perturbations in low-frequency neural activity. Read More

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A Combined Study of Tc Redox Speciation in Complex Aqueous Systems: Wet-Chemistry, Tc K-/L-Edge X-ray Absorption Fine Structure, and Ab Initio Calculations.

Inorg Chem 2021 Jul 30. Epub 2021 Jul 30.

Karlsruhe Institute of Technology (KIT), Institute for Nuclear Waste Disposal (INE), P.O. Box 3640, D-76021 Karlsruhe, Germany.

The combination of wet-chemistry experiments (measurements of pH, , and [Tc]) and advanced spectroscopic techniques (K- and L-edge X-ray absorption fine structure spectroscopy) confirms the formation of a very stable Tc(V)-gluconate complex under anoxic conditions. In the presence of gluconate and an excess of Sn(II) (at pe + pH ≈ 2), technetium forms a very stable Tc(IV)-gluconate complex significantly enhancing the solubility defined by TcO(s) in hyperalkaline gluconate-free systems. A new setup for "tender" X-ray spectroscopy (spectral range, ∼2-5 keV) in transmission or total fluorescence yield detection mode based on a He flow cell has been developed at the INE Beamline for radionuclide science (KIT light source). Read More

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Probl Sotsialnoi Gig Zdravookhranenniiai Istor Med 2021 Jun;29(Special Issue):680-683

N. A. Kosygin Russian State University (Technologies. Design. Art), 117997, Moscow, Russia,

The global coronavirus pandemic (COVID-19) has led to changes in advertising, marketing, advertising spending and media activities, forcing companies and brands to rethink their views on current and future advertising and marketing campaigns in order to maintain a stable revenue stream. This becomes even more important, because as a result of COVID-19, it is expected that starting in 2020, in the future, at least for the next 4-5 years, advertising costs for some advertisers will decrease as revenue decreases and the transition to other forms and channels of communication with the target audience of consumers. As a result, many brands may seek to optimize their marketing practices to better reflect the growth of online transactions, communication, and personal interaction time with consumers. Read More

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Photocurable bioresorbable adhesives as functional interfaces between flexible bioelectronic devices and soft biological tissues.

Nat Mater 2021 Jul 29. Epub 2021 Jul 29.

Chemistry Life Processes Institute, Northwestern University, Evanston, IL, USA.

Flexible electronic/optoelectronic systems that can intimately integrate onto the surfaces of vital organ systems have the potential to offer revolutionary diagnostic and therapeutic capabilities relevant to a wide spectrum of diseases and disorders. The critical interfaces between such technologies and living tissues must provide soft mechanical coupling and efficient optical/electrical/chemical exchange. Here, we introduce a functional adhesive bioelectronic-tissue interface material, in the forms of mechanically compliant, electrically conductive, and optically transparent encapsulating coatings, interfacial layers or supporting matrices. Read More

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Characterizing change in vagal tone during the first three years of life: A systematic review and empirical examination across two longitudinal samples.

Neurosci Biobehav Rev 2021 Jul 26. Epub 2021 Jul 26.

University of North Carolina at Chapel Hill, United States.

Functioning of the parasympathetic nervous system (PNS), most often indexed by respiratory sinus arrhythmia (RSA), influences the volitional, cognitively-mediated forms of self-regulation across development. However, despite its clear relevance to children's self-regulation, and its utility as a transdiagnostic biomarker of emotion dysregulation and psychopathology, the ontogeny of vagal tone under conditions of homeostasis across infancy and early childhood is not well understood. The current research is comprised of two complementary studies. Read More

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Sulfate migration and transformation characteristics in paddy soil profile affected by acid mine drainage.

Environ Res 2021 Jul 26:111732. Epub 2021 Jul 26.

School of Environment and Energy, South China University of Technology, Guangzhou, 510006, PR China; Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, South China University of Technology, Guangzhou, 510006, PR China.

SO, a major component of acid mine drainage (AMD), plays an important role in study environment of AMD. We investigated the distribution and adsorption-desorption mechanisms of SO and the variation of stable isotope of sulfur (δS) values in the soil profile polluted by AMD. Results showed that the species and S values of SO differed significantly among different soil depths. Read More

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Reliability, Validity, Clinical Utility, and Responsiveness of Measures for Assessing Mobility and Physical Function in Patients with Traumatic Injury in the Acute Care Hospital Setting: A Prospective Study.

Phys Ther 2021 Jul 29. Epub 2021 Jul 29.

Department of Physiotherapy, The Alfred Hospital, Prahran, Melbourne, VIC 3181.

Objective: The longer-term impact of injury is increasingly recognized, but the early phases of recovery are less well understood. The best tools to measure early recovery of mobility and physical function following traumatic injury are unclear. The purpose of this study was to assess the clinical utility, validity, reliability, and responsiveness of 4 mobility and physical function measures in patients following traumatic injury. Read More

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To Join or Not to Join: Decision Points Along the Pathway to Double-Strand Break Repair vs. Chromosome End Protection.

Front Cell Dev Biol 2021 12;9:708763. Epub 2021 Jul 12.

Department of Biological Sciences, University of South Carolina, Columbia, SC, United States.

The regulation of DNA double-strand breaks (DSBs) and telomeres are diametrically opposed in the cell. DSBs are considered one of the most deleterious forms of DNA damage and must be quickly recognized and repaired. Telomeres, on the other hand, are specialized, stable DNA ends that must be protected from recognition as DSBs to inhibit unwanted chromosome fusions. Read More

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Alternatives to Traditional Pedicle Screws for Posterior Fixation of the Degenerative Lumbar Spine.

JBJS Rev 2021 07 28;9(7). Epub 2021 Jul 28.

San Francisco Orthopaedic Residency Program, St. Mary's Medical Center, San Francisco, California.

Background: Traditional pedicle screws are currently the gold standard to achieve stable 3-column fixation of the degenerative lumbar spine. However, there are cases in which pedicle screw fixation may not be ideal. Due to their starting point lateral to the pars interarticularis, pedicle screws require a relatively wide dissection along with a medialized trajectory directed toward the centrally located neural elements and prevertebral vasculature. Read More

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Functional Myoglobin Model Composed of a Strapped Porphyrin/Cyclodextrin Supramolecular Complex with an Overhanging COOH That Increases O/CO Binding Selectivity in Aqueous Solution.

Inorg Chem 2021 Jul 28. Epub 2021 Jul 28.

Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, Kyotanabe-city, Kyoto 610-0321, Japan.

A water-soluble strapped iron(III)tetraarylporphyrin () bearing two propylpyridinium groups at the side chains and a carboxylic acid group at the overhanging position of the strap was synthesized to mimic the function of myoglobin with the distal polar functionality in aqueous solution. forms a stable 1:1 inclusion complex with a per--methylated β-cyclodextrin dimer having a pyridine linker (), providing a hydrophobic environment and a proximal fifth ligand to stabilize the O-complex. The ferrous complex () binds both O and CO in aqueous solution. Read More

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Mechanical studies of the solid electrolyte interphase on anodes in lithium and lithium ion batteries.

Nanotechnology 2021 Jul 27. Epub 2021 Jul 27.

Department of Chemistry, University of Utah, Salt Lake City , Utah, UNITED STATES.

A stable solid electrolyte interphase (SEI) layer is key to high performing lithium ion batteries for metrics such as calendar and cycle life. The SEI must be mechanically robust to withstand large volumetric changes in anode materials such as lithium and silicon, so understanding the mechanical properties and behavior of the SEI is essential for the rational design of artificial SEI and anode form factors. The mechanical properties and mechanical failure of the SEI are challenging to study, because the SEI is thin at only ~ 10 - 200 nm thick and is air sensitive. Read More

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Solution Epitaxy of Halide Perovskite Thin Single Crystals for Stable Transistors.

ACS Appl Mater Interfaces 2021 Jul 27. Epub 2021 Jul 27.

School of Physics, Australian Centre for Microscopy and Microanalysis, Sydney Nano Institute, The University of Sydney, Sydney, New South Wales 2006, Australia.

Halide perovskites hold promise for energy and optoelectronic applications due to their fascinating photophysical properties and facile processing. Among various forms, epitaxial thin single crystals (TSCs) are highly desirable due to their high crystallinity, reduced defects, and easy epitaxial integration with other materials. However, a cost-effective method for obtaining TSCs with perfect epitaxial features remains elusive. Read More

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In search of the optimal enrichment program for zoo-housed animals.

Zoo Biol 2021 Jul 26. Epub 2021 Jul 26.

Scientific Research Department, Moscow Zoo, Moscow, Russia.

Zoo-housed animals are regularly exposed to new forms of environmental enrichment to make their lives less predictable. However, providing new enrichment can have unpredictable effects. We evaluated the effectiveness of two enrichment regimes: (1) providing only new enrichments - "Novelty" and (2) only familiar enrichments - "Familiar. Read More

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Intron-retained radish (Raphanus sativus L.) RsMYB1 transcripts found in colored-taproot lines enhance anthocyanin accumulation in transgenic Arabidopsis plants.

Plant Cell Rep 2021 Jul 25. Epub 2021 Jul 25.

Department of Biological Sciences, College of Biological Science and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea.

Key Message: Overexpression of the naturally occurring intron-retained (IR) forms of radish RsMYB1 and RsTT8 transcripts in Arabidopsis causes a substantial increase in anthocyanin accumulation. The production of anthocyanins in plants is tightly controlled by the MYB-bHLH-WD40 (MBW) complex. In this study, analysis of four radish (Raphanus sativus L. Read More

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Structural and Biochemical Characterization of the Flavin-Dependent Siderophore-Interacting Protein from .

ACS Omega 2021 Jul 6;6(28):18537-18547. Epub 2021 Jul 6.

Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061, United States.

is an opportunistic pathogen with a high mortality rate due to multi-drug-resistant strains. The synthesis and uptake of the iron-chelating siderophores acinetobactin (Acb) and preacinetobactin (pre-Acb) have been shown to be essential for virulence. Here, we report the kinetic and structural characterization of BauF, a flavin-dependent siderophore-interacting protein (SIP) required for the reduction of Fe(III) bound to Acb/pre-Acb and release of Fe(II). Read More

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Advances and new frontiers for immunotherapy in colorectal cancer: Setting the stage for neoadjuvant success?

Mol Ther Oncolytics 2021 Sep 14;22:1-12. Epub 2021 May 14.

Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota, 420 Delaware St., SE, MMC 480, Minneapolis, MN 55455, USA.

Immunotherapy in the metastatic setting has drastically altered the landscape of treatment for various types of malignancy, including colorectal cancer. The category of immune checkpoint inhibitors has especially emerged as a class of therapy predicated on a more comprehensive understanding of immune cell-cancer cell regulation and evolution of the tumor microenvironment over time. Strategies including adoptive cellular therapies, tumor vaccines, and antibodies have also demonstrated the ability to enhance antitumor immunity. Read More

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September 2021

NOX66 as Monotherapy, and in Combination With Carboplatin, in Patients With Refractory Solid Tumors: Phase Ia/b Study.

Curr Ther Res Clin Exp 2021 28;94:100631. Epub 2021 Mar 28.

Noxopharm Limited, Gordon, New South Wales, Australia.

Background: Although oral and intravenous forms of idronoxil have been well tolerated, the safety of NOX66, with idronoxil formulated as a rectal suppository, is not known. This Phase Ia/b clinical study (protocol No. NOX66-001A), known as Chemotherapy Enhancement Program-1, is the first to assess NOX66 in patients with refractory solid tumors. Read More

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Simultaneous determination of twenty-five compounds with anti-inflammatory activity in Spatholobi Caulis by using an optimized UFLC-MS/MS method: An application to pharmacokinetic study.

J Pharm Biomed Anal 2021 Jul 14;204:114267. Epub 2021 Jul 14.

Zhuzhou Qianjin Pharmaceutical Co., Ltd., Zhuzhou 412003, China.

As a kind of commonly used Traditional Chinese Medicine in clinical, Spatholobi Caulis (SPC) contains a wide variety of bioactive compounds, including protocatechuate (1), nicotinic acid (2), p-hydroxybenzoic acid (3), salicylic acid (4), 6,9-dihydroxy megastigma-4,7-dien-3-one (5), 8,9-dihydroxy megastigma-4,6-dien-3-one (6), daidzin (7), genistin (8), isolariciresinol (9), ononin (10), 4',8-dimethoxy-7-O-β-d-glucopyranosyl isoflavone (11), 3'-methoxydaidzein (12), odoratin (13), spasuberol A (14), (+)-pinoresinol (15), 4-hydroxy-3-methoxy cinnamic acid methyl ester (16), (+)-epipinoresinol (17), calycosin (18), 8-O-methylretusin (19), formononetin sodium (20), formononetin (21), biochanin A (22), butesuperin A (23), homovanillyl-4-oxo-nonanoate (24) and (6aR,11aR)-maackiain (25). The pharmacokinetic characteristics of these twenty-five compounds in rat plasma were quantitatively and simultaneously studied using a fast, sensitive and precise ultra fast liquid chromatography combined with electrospray ionization triple quadrupole tandem mass spectrometry (UFLC-MS/MS) method after oral administration of aqueous extract of SPC to rats. The mobile phase consists of acetonitrile and 0. Read More

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Cryo-EM structures of LptBFG and LptBFGC from Klebsiella pneumoniae in complex with lipopolysaccharide.

Biochem Biophys Res Commun 2021 Jul 21;571:20-25. Epub 2021 Jul 21.

Department of Clinical Laboratory, Shenzhen Baoan Women's and Children's Hospital, Jinan University, Shenzhen, 518133, China. Electronic address:

Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most Gram-negative bacteria. LPS transport from the inner membrane (IM) to the OM is achieved by seven lipopolysaccharide transport proteins (LptA-G). LptBFG, an type VI ATP-binding cassette (ABC) transporter, forms a stable complex with LptC, extracts LPS from the IM and powers LPS transport to the OM. Read More

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The treatment of electroplating wastewater using an integrated approach of interior microelectrolysis and Fenton combined with recycle ferrite.

Chemosphere 2021 Jul 13;286(Pt 1):131543. Epub 2021 Jul 13.

School of the Environment and Safety Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, China; Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, T6G 1H9, Canada. Electronic address:

Heavy metal ions in chelated forms have aroused great concerns because of their high solubility, poor biodegradation and extreme stability. In this research, an efficient strategy, interior microelectrolysis-Fenton-recycle ferrite (IM-Fenton-RF), was developed to treat simulated electroplating wastewater containing chelated copper at room temperature. The decomplexation of chelated copper was carried out by both interior microelectrolysis and Fenton reactions. Read More

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Dissecting primary and secondary senescence to enable new senotherapeutic strategies.

Ageing Res Rev 2021 Jul 21;70:101412. Epub 2021 Jul 21.

SENS Research Foundation, Mountain View, CA, USA; Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Loughborough, UK. Electronic address:

Cellular senescence is a state of stable cell cycle arrest that is known to be elicited in response to different stresses or forms of damage. Senescence limits the replication of old, damaged, and precancerous cells in the short-term but is implicated in diseases and debilities of aging due to loss of regenerative reserve and secretion of a complex combination of factors called the senescence-associated secretory phenotype (SASP). More recently, investigators have discovered that senescent cells induced by these methods (what we term "primary senescent cells") are also capable of inducing other non-senescent cells to undergo senescence - a phenomenon we call "secondary senescence. Read More

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Reaction of Chalcones with Cellular Thiols. The Effect of the 4-Substitution of Chalcones and Protonation State of the Thiols on the Addition Process. Diastereoselective Thiol Addition.

Molecules 2021 Jul 17;26(14). Epub 2021 Jul 17.

Institute of Pharmaceutical Chemistry, University of Pécs, H-7624 Pécs, Hungary.

Several biological effects of chalcones have been reported to be associated with their thiol reactivity. In vivo, the reactions can result in the formation of small-molecule or protein thiol adducts. Both types of reactions can play a role in the biological effects of this class of compounds. Read More

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Structure and IR Spectra of 3(5)-Aminopyrazoles and UV-Induced Tautomerization in Argon Matrix.

Molecules 2021 Jul 15;26(14). Epub 2021 Jul 15.

CQC-IMS, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal.

The prototropic tautomerism in 3(5)-aminopyrazoles was investigated by matrix isolation infrared (IR) spectroscopy, supported by DFT(B3LYP)/6-311++G(d,p) calculations. In consonance with the experimental data, the calculations predict tautomer 3-aminopyrazole (3AP) to be more stable than the 5-aminopyrazole (5AP) tautomer (calculated energy difference: 10.7 kJ mol; Gibbs free energy difference: 9. Read More

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PXR Modulates the Prostate Cancer Cell Response to Afatinib by Regulating the Expression of the Monocarboxylate Transporter SLC16A1.

Cancers (Basel) 2021 Jul 20;13(14). Epub 2021 Jul 20.

IRCM, Institut de Recherche en Cancérologie de Montpellier, INSERM U1194, Université de Montpellier, ICM, F-34298 Montpellier, France.

Resistance to castration is a crucial issue in the treatment of metastatic prostate cancer. Kinase inhibitors (KIs) have been tested as potential alternatives, but none of them are approved yet. KIs are subject of extensive metabolism at both the hepatic and the tumor level. Read More

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Predictive and Prognostic Biomarker Identification in a Large Cohort of Androgen Receptor-Positive Salivary Duct Carcinoma Patients Scheduled for Combined Androgen Blockade.

Cancers (Basel) 2021 Jul 14;13(14). Epub 2021 Jul 14.

Department of Urology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6525GA Nijmegen, The Netherlands.

Patients suffering from recurrent or metastatic (R/M) salivary duct carcinoma (SDC) are often treated with combined androgen blockade (CAB). However, CAB frequently fails, resulting in a worse prognosis. Therefore, biomarkers that can predict treatment failure are urgently needed. Read More

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Binder-free twin-screw melt granulation: an effective approach to manufacture high-dose API formulations.

Int J Pharm 2021 Jul 20:120886. Epub 2021 Jul 20.

C-SOPS, Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, 98 Brett Road, Piscataway, NJ 08854, USA. Electronic address:

This study investigates the use of twin-screw binder-free melt granulation (BFMG) in the development of high-dose solid dose formulations for low melting point thermally stable drugs. Both ibuprofen and guaifenesin are examined. By granulating pure API powder, it is shown that BFMG can successfully be used to produce granules that contain 100% API. Read More

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Full-length G Glycoprotein directly extracted from Rabies Virus with Detergent and then Stabilized by Amphipols in Liquid and Freeze-dried Forms.

Biotechnol Bioeng 2021 Jul 23. Epub 2021 Jul 23.

Bioprocess R&D Department, Sanofi Pasteur, Marcy l'Etoile, France.

Pathogen surface antigens are at the forefront of the viral strategy when invading host organisms. These antigens, including membrane proteins (MPs), are broadly targeted by the host immune response. Obtaining these MPs in a soluble and stable form constitutes a real challenge, regardless of the application purposes (e. Read More

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