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Novel hyperthermophilic crenarchaeon Infirmifilum lucidum gen. nov. sp. nov., reclassification of Thermofilum uzonense as Infirmifilum uzonense comb. nov. and assignment of the family Thermofilaceae to the order Thermofilales ord. nov.

Syst Appl Microbiol 2021 Jul 9;44(4):126230. Epub 2021 Jul 9.

Winogradsky Institute of Microbiology, Research Center of Biotechnology RAS, 7/2 Prospekt 60-letiya Oktyabrya, 117312 Moscow, Russia.

A novel hyperthermophilic crenarchaeon, strain 3507LT, was isolated from a terrestrial hot spring near Tinguiririca volcano, Chile. Cells were non-motile thin, slightly curved filamentous rods. It grew at 73-93 °C and pH range of 5 to 7. Read More

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Precipitation and transformation of σ phase in a selective laser melted high-chromium superalloy subjected to heat treatment.

Micron 2021 Jul 15;149:103113. Epub 2021 Jul 15.

State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, PR China.

A commercial high-chromium contained GH648 superalloy was fabricated by selective laser melting (SLM), followed by solution treatment at 1140 °C for 1 h and subsequent aging treatment at 900 °C for 16 h. The precipitation and transformation of σ phase has been characterized. It has been shown that σ precipitates formed after solution treatment, attributed to the segregation of Mo element in the subgrain boundaries and also the difference between the diffusion ability of subgrain boundaries and lattice. Read More

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Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17.

Protein Expr Purif 2021 Jul 19:105944. Epub 2021 Jul 19.

Laboratory of Protein Conformation and Dynamics, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, United States. Electronic address:

The pre-melanosomal protein (Pmel17) is a human functional amyloid that supports melanin biosynthesis within melanocytes. This occurs in the melanosome, a membrane-bound organelle with an acidic intraluminal pH. The repeat region of Pmel17 (RPT, residues 315-444) has been previously shown to form amyloid aggregates under acidic melanosomal conditions, but not under neutral cytosolic conditions, when expressed and purified using a C-terminal hexa-histidine tag (RPT-His). Read More

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Liquid-liquid phase separation as a common organizing principle of intracellular space and biomembranes providing dynamic adaptive responses.

Biochim Biophys Acta Mol Cell Res 2021 Jul 19:119102. Epub 2021 Jul 19.

Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Institutskiy pereulok, 9, Dolgoprudny, 141700, Russia; Department of Molecular Medicine and USF Health Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd., MDC07, Tampa, FL 33612, USA. Electronic address:

This work is devoted to the phenomenon of liquid-liquid phase separation (LLPS), which has come to be recognized as fundamental organizing principle of living cells. We distinguish separation processes with different dimensions. Well-known 3D-condensation occurs in aqueous solution and leads to membraneless organelle (MLOs) formation. Read More

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A boronic acid-modified C derivatization reagent for the rapid detection of 3-monochloropropane-1,2-diol using MALDI mass spectrometry.

Rapid Commun Mass Spectrom 2021 Jul 22:e9169. Epub 2021 Jul 22.

Department of Chemistry, Wuhan University, Wuhan, P.R. China.

Rationale: 3-Monochloropropane-1,2-diol (3-MCPD) is a well-known contaminant formed in food thermal processing, which could be found in a variety of foodstuffs. Due to its potential carcinogenicity, it was essential to quickly develop a rapid and high-throughput analytical method to monitor 3-MCPD in foodstuffs, which is described in this study.

Methods: 3-MCPD was extracted from foodstuffs and then was derivatized with a boronic acid-modified C (B-C ) through the boronic acid-diol reaction. Read More

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Platelet EVs contain an active proteasome involved in protein processing for antigen presentation via MHC-I molecules.

Blood 2021 Jul 22. Epub 2021 Jul 22.

CHU de Quebec, Quebec, Quebec, Canada.

In addition to their hemostatic role, platelets play a significant role in immunity. Once activated, platelets release extracellular vesicles (EVs) formed by budding of their cytoplasmic membranes. Because of their heterogeneity, platelet EVs (PEVs) are thought to perform diverse functions. Read More

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A tightly clustered hepatitis E virus genotype 1a is associated with endemic and outbreak infections in Bangladesh.

PLoS One 2021 22;16(7):e0255054. Epub 2021 Jul 22.

Oxford University Clinical Research Unit, Wellcome Asia Programme, The Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.

Background: Hepatitis E virus (HEV) infection is endemic in Bangladesh and there are occasional outbreaks. The molecular characteristics and pathogenesis of endemic and outbreak HEV strains are poorly understood. We compared the genetic relatedness and virulence associated mutations of endemic HEV strains with outbreak strains. Read More

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Service-learning: experience of teacher-tutors in the nursing career.

Medwave 2021 Jul 14;21(6):e8218. Epub 2021 Jul 14.

Instituto Enfermería, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile. Adress: Isla Teja sin número, Campus Isla Teja, Universidad Austral de Chile, Valdivia, Chile. Email: ORCID: 0000-0002-2403-5756.

Introduction: The learning process demands appropriate and effective strategies. Furthermore, the service-learning methodology implies significant challenges for both the student and the teacher-tutor.

Objective: To reveal the experience of community-based teacher-tutors work with nursing students from the Austral University of Chile, using the service-learning methodology. Read More

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Collaborative governance of postgraduate medical education: Can it be achieved?

Med Teach 2021 Jul 22:1-6. Epub 2021 Jul 22.

Department of Family Practice, University of British Columbia, Vancouver, BC, Canada.

Faced with the need to modernize and improve the postgraduate medical education experience and to maintain the high quality of physicians that Canadians expect, in 2010, four organizations -Association of Faculties of Medicine of Canada (AFMC); Collège des Médecins du Québec (CMQ); College of Family Physicians of Canada (CFPC); and Royal College of Physicians and Surgeons of Canada (RCPSC) formed a consortium to conduct a review of Postgraduate Medical Education (PGME) in Canada. In 2012, the Consortium published the Future of Medical Education in Canada Postgraduate (FMEC PG) project's 10 recommendations for change in PGME. One of these recommendations was to 'Establish Effective Collaborative Governance in PGME'. Read More

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Structural Insights into Notum Covalent Inhibition.

J Med Chem 2021 Jul 22. Epub 2021 Jul 22.

Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, U.K.

The carboxylesterase Notum hydrolyzes a palmitoleate moiety from Wingless/Integrated(Wnt) ligands and deactivates Wnt signaling. Notum inhibitors can restore Wnt signaling which may be of therapeutic benefit for pathologies such as osteoporosis and Alzheimer's disease. We report the identification of a novel class of covalent Notum inhibitors, 4-(indolin-1-yl)-4-oxobutanoate esters. Read More

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Independent and Synergistic Modulations of Viscoelasticity and Stiffness of Dynamically Cross-Linked Cell-Encapsulating ClickGels by Covalently Tethered Polymer Brushes.

Authors:
Yu Tan Jie Song

Biomacromolecules 2021 Jul 22. Epub 2021 Jul 22.

Department of Orthopedics & Physical Rehabilitation, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, United States.

We report independent and synergistic modulations of the stiffness and viscoelasticity of ClickGels, formed by a combination of the bio-orthogonal covalent and dynamic noncovalent cross-linking, by covalently incorporating nonionic, zwitterionic, or anionic polymer brushes. Tethering nonionic and zwitterionic brushes at the cost of noncovalent cross-linking increased stiffness and slowed stress relaxation, respectively, without altering the other properties. Meanwhile, tethering anionic brushes significantly increased ClickGel stiffness, while also slowing its stress relaxation. Read More

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3'-Terminal Repair-Powered Dendritic Nanoassembly of Polyadenine Molecular Beacons for One-Step Quantification of Alkaline Phosphatase in Human Serum.

Anal Chem 2021 Jul 22. Epub 2021 Jul 22.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.

Alkaline phosphatase (ALP) is an important hydrolase with crucial roles in biological processes, and the dysregulation of ALP may cause various human diseases. The conventional ALP assays usually involve cumbersome procedures with poor sensitivity. Herein, taking advantage of intrinsic superiorities of molecular beacons (MBs) and unique features of terminal deoxynucleotidyl transferase (TdT), we demonstrate for the first time the 3'-terminal repair-powered dendritic nanoassembly of polyadenine (A) MBs for one-step quantification of ALP in human serum. Read More

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Molecular Engineering of Hydrogels for Rapid Water Disinfection and Sustainable Solar Vapor Generation.

Adv Mater 2021 Jul 22:e2102994. Epub 2021 Jul 22.

Materials Science and Engineering Program and Department of Mechanical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA.

Consumption of unsafe water is a major cause of morbidity and mortality in developing regions. Pasteurizing or boiling water to remove pathogens is energy-intensive and often impractical to off-grid communities. Therefore, low capital cost, rapid and energy-efficient water disinfection methods are urgently required to address global challenges of safe water access. Read More

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Can the cytokine adsorber CytoSorb help to mitigate cytokine storm and reduce mortality in critically ill patients? A propensity score matching analysis.

Ann Intensive Care 2021 Jul 22;11(1):115. Epub 2021 Jul 22.

Department of Anesthesiology, University Hospital LMU Munich, Marchioninistrasse 15, 81377, Munich, Germany.

Background: A cytokine storm is life threatening for critically ill patients and is mainly caused by sepsis or severe trauma. In combination with supportive therapy, the cytokine adsorber Cytosorb (CS) is increasingly used for the treatment of cytokine storm. However, it is questionable whether its use is actually beneficial in these patients. Read More

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Raman imaging of Micrasterias: new insights into shape formation.

Protoplasma 2021 Jul 22. Epub 2021 Jul 22.

Department of Nanobiotechnology, University of Natural Resources and Life Sciences Vienna (BOKU), 1190, Vienna, Austria.

The algae Micrasterias with its star-shaped cell pattern is a perfect unicellular model system to study morphogenesis. How the indentations are formed in the primary cell wall at exactly defined areas puzzled scientists for decades, and they searched for chemical differences in the primary wall of the extending tips compared to the resting indents. We now tackled the question by Raman imaging and scanned in situ Micrasterias cells at different stages of development. Read More

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Signal strength controls the rate of polarization within CTLs during killing.

J Cell Biol 2021 Oct 22;220(10). Epub 2021 Jul 22.

Cambridge Institute for Medical Research, Biomedical Campus, Cambridge, UK.

Cytotoxic T lymphocytes (CTLs) are key effector cells in the immune response against viruses and cancers, killing targets with high precision. Target cell recognition by CTL triggers rapid polarization of intracellular organelles toward the synapse formed with the target cell, delivering cytolytic granules to the immune synapse. Single amino acid changes within peptides binding MHC class I (pMHCs) are sufficient to modulate the degree of killing, but exactly how this impacts the choreography of centrosome polarization and granule delivery to the target cell remains poorly characterized. Read More

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October 2021

Pattern And Prevalence of Liver Involvement in Pediatric Acute Lymphoblastic and Myeloid Leukemia at Diagnosis.

J Pediatr Gastroenterol Nutr 2021 Jul 20. Epub 2021 Jul 20.

Department of Pediatrics, Karolinska University Hospital, Stockholm, Sweden Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden Department of Pediatrics, Karolinska University Hospital and Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden Department of Pediatrics, Karolinska University Hospital and CLINTEC Karolinska Institutet, Stockholm, Sweden Department of Pediatrics, University of Helsinki, Helsinki, Finland.

Objectives: The prevalence and significance of liver involvement at diagnosis was studied in pediatric acute lymphoblastic (ALL) and myeloid leukemia (AML).

Methods: A population based cohort of 122 pre B-ALL, 22 T-ALL and 45 AML patients was formed from the Nordic Society of Pediatric Hematology and Oncology leukemia registries (years 2005 - 2017). Hepatomegaly, elevated ALT, high INR, hypoalbuminemia and conjugated hyperbilirubinemia at diagnosis were used as markers for liver involvement. Read More

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sp. nov., isolated from marine sediment.

Int J Syst Evol Microbiol 2021 Jul;71(7)

Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.

A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterial strain (CAU 1508) was isolated from marine sediment collected in the Republic of Korea. Growth was observed at 10-45 °C (optimum, 30 °C), pH 4.0-11. Read More

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Understanding Heart Rate Reactions to Post-Traumatic Stress Disorder (PTSD) Among Veterans: A Naturalistic Study.

Hum Factors 2021 Jul 22:187208211034024. Epub 2021 Jul 22.

265514736 Texas A&M University, College Station, USA.

Objective: We collected naturalistic heart rate data from veterans diagnosed with post-traumatic stress disorder (PTSD) to investigate the effects of various factors on heart rate.

Background: PTSD is prevalent among combat veterans in the United States. While a positive correlation between PTSD and heart rate has been documented, specific heart rate profiles during the onset of PTSD symptoms remain unknown. Read More

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-Tris(heteroleptic) Red Phosphorescent Iridium(III) Complexes Bearing a Dianionic ,,','-Tetradentate Ligand.

Inorg Chem 2021 Jul 22. Epub 2021 Jul 22.

Universal Display Corporation, Ewing, New Jersey 08618, United States.

1-Phenyl-3-(1-phenyl-1-(pyridin-2-yl)ethyl)isoquinoline (HMeL) has been prepared by Pd(-XantPhos)-catalyzed "" to synthesize new phosphorescent red iridium(III) emitters (601-732 nm), including the carbonyl derivative Ir(κ--,'--,'-MeL)Cl(CO) and the acetylacetonate compound Ir(κ--,'--,'-MeL)(acac). The tetradentate 6e-donor ligand (6tt') of these complexes is formed by two different bidentate units, namely, an orthometalated 2-phenylisoquinoline and an orthometalated 2-benzylpyridine. The link between the bidentate units reduces the number of possible stereoisomers of the structures [6tt' + 3b] (3b = bidentate 3e-donor ligand), with respect to a [3b + 3b' + 3b″] emitter containing three free bidentate units, and it permits a noticeable stereocontrol. Read More

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Physicochemical aspects of design of ultrathin films based on chitosan, pectin, and their silver nanocomposites with antiadhesive and bactericidal potential.

J Biomed Mater Res A 2021 Jul 22. Epub 2021 Jul 22.

Laboratory of micro- and nanostructured systems, Institute of chemistry of new materials National academy of sciences of Belarus, 36 F. Skaryna str, Minsk, 220141, Belarus.

Implant-related infection is one of the serious problems in regenerative medicine. Promising approach to overcome the problems caused by bacterial growth on the medical implants is their modification by bioactive coatings. A versatile technique for designing multilayer films with tailored characteristics at the nanometer scale is layer-by-layer assembly. Read More

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T-Regulatory Cells in Erythema Nodosum Leprosum: An Immunohistochemical and Image Morphometric Study.

Am J Dermatopathol 2021 Jul 20. Epub 2021 Jul 20.

Departments of Pathology and Lab Medicine, and. Dermatology, AIIMS, Bhubaneswar, Odisha.

Abstract: Erythema nodosum leprosum (ENL) occurs as an immune-inflammatory complication of multibacillary leprosy (MBL), precipitated by an interaction between the host, bacilli, and the environment. This complication often causes significant morbidity due to systemic involvement and needs to be treated aggressively. T-regulatory cells (T-regs) are the immunomodulatory subset of T cells that are hypothesized to play a role in ENL. Read More

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Giant Pilomatrical Tumor With Broad Epidermal Components: An Example of Histological Diversity and a Potential Diagnostic Pitfall of Tumors With Pilomatrical Differentiation.

Am J Dermatopathol 2021 Jul 20. Epub 2021 Jul 20.

Departments of Diagnostic Pathology, and Dermatology, Kyoto University Hospital, Kyoto, Japan; Department of Dermatology, Kagawa University Hospital, Kagawa, Japan; and National Hospital Organization Kyoto Medical Center, Kyoto, Japan.

Abstract: The diagnosis of pilomatricoma, the most common matrical tumor, is generally straightforward; however, it exhibits diverse histology associated with various morphological stages and several clinical variants, and matrical differentiation can occur in various neoplastic diseases. A 56-year-old man was admitted to our hospital to resect an 11.0-cm skin tumor on his right shoulder. Read More

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Cultivating the physician workforce: Recruiting, training, and retaining physicians to meet the needs of the population.

Med Teach 2021 Jul;43(sup2):S39-S48

University of California Davis School of Medicine, Sacramento, CA, USA.

In the 10 years since the Lancet Commission on Education of Health Professionals for the 21st Century suggested the changes necessary to transform medical education, the United States remains plagued by shortages of physicians and maldistribution of the physician workforce. Minoritized and rural communities usually suffer the most, with widely documented health disparities across the United States by race, ethnicity, gender identity, education, and zip code. Medical schools can respond by recruiting students more likely to practice in these settings and training them to address the community needs. Read More

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Upgrading of Ethanol to 1,1-Diethoxyethane by Proton Exchange Membrane Electrolysis.

ChemSusChem 2021 Jul 21. Epub 2021 Jul 21.

Saitama University Graduate School of Science and Engineering: Saitama Daigaku Daigakuin Rikogaku Kenkyuka, Applied Chemistry, JAPAN.

The direct acetalization of ethanol is a significant challenge for upgrading bioethanol to value-added chemicals. Herein, 1,1-diethoxyethane (DEE) was selectively synthesized by the electrolysis of ethanol using a proton exchange membrane (PEM) reactor. In the PEM reactor, a Pt/C catalyst promoted the electro-oxidation of ethanol to acetaldehyde. Read More

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The complete chloroplast genome of , an orchid with important ornamental value and medicinal value.

Mitochondrial DNA B Resour 2021 9;6(8):2263-2265. Epub 2021 Jul 9.

School of Life Sciences, Yunnan Normal University, Kunming, China.

is an epiphytic orchid with significant ornamental value and medicinal value. Here, we report the first complete chloroplast genome of . The circular genome was 158,394 bp in length and consisted of a pair of inverted repeats (IR 26,646 bp), which were separated by a large single copy region (LSC 86,603 bp) and a small single copy region (SSC 18,499 bp). Read More

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Analysis of Milk Using a Portable Potentiometric Electronic Tongue Based on Five Polymeric Membrane Sensors.

Front Chem 2021 5;9:706460. Epub 2021 Jul 5.

Group UVASENS, Escuela de Ingenierías Industriales, Universidad de Valladolid, Valladolid, Spain.

A portable potentiometric electronic tongue (PE-tongue) was developed and applied to evaluate the quality of milk with different fat content (skimmed, semi-skimmed, and whole) and with different nutritional content (classic, calcium-enriched, lactose-free, folic acid-enriched, and enriched in sterols of vegetal origin). The system consisted of a simplified array of five sensors based on PVC membranes, coupled to a data logger. The five sensors were selected from a larger set of 20 sensors by applying the genetic algorithm (GA) to the responses to compounds usually found in milk including salts (KCl, CaCl, and NaCl), sugars (lactose, glucose, and galactose), and organic acids (citric acid and lactic acid). Read More

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Continued use of Warfarin in lower dose has safe maternal and neonatal outcomes in pregnant women with Prosthetic Heart Valves.

Pak J Med Sci 2021 Jul-Aug;37(4):933-938

Anjum Jalal, FRCS-CTh. Professor of Cardiac Surgery, Executive Director, Faisalabad Institute of Cardiology, Faisalabad, Pakistan.

Background And Objective: There has been concerns regarding the safety of Warfarin in pregnant females due to its teratogenic potential. At the same time warfarin provides best anticoagulation in patients with prosthetic valves. Various dosage regimes have been tried to strike a balance between safety of mother and the avoidance of congenital anomalies in the newborn. Read More

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November 2020

Co-harboring of Novel Plasmid and Integrative and Conjugative Element Carrying Tn in Multidrug-Resistant .

Front Microbiol 2021 5;12:674974. Epub 2021 Jul 5.

Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Many strains of the opportunistic pathogen have acquired resistance to multiple antibiotics. Carbapenem-resistant poses a global healthcare problem due to limited therapeutic options for the treatment of infections. Plasmids and integrative and conjugative elements (ICEs) are the major vectors of antibiotic-resistance gene transfer. Read More

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Whole Resistome Analysis in and Genomes Available in Public Repositories.

Front Microbiol 2021 5;12:662144. Epub 2021 Jul 5.

Department of Food Hygiene and Technology, Universidad de León, León, Spain.

spp. are the most frequent agent of human gastroenteritis worldwide, and the spread of multidrug-resistant strains makes the clinical treatment difficult. The current study presents the resistome analysis of 39,798 and 11,920 genomes available in public repositories. Read More

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