324,190 results match your criteria form cellular

Fabricating Efficient and Stable Quasi-3D and 3D/2D Perovskite Solar Cells with 2D-Sheets Connected by Inorganic Type Ionic-Bond.

Nanotechnology 2021 May 11. Epub 2021 May 11.

School of Chemistry and Materials Science, Nanjing University of Information Science and Technology, Nanjing, Jiangsu, CHINA.

In this work, we report a novel two dimensional (2D) (SrBr)2PbI4 perovskite layered architecture, which were formed by the reaction of strontium bromide (SrBr2), strontium iodide (SrI2) and lead iodide (PbI2). Formation of 2D (SrBr)2PbI4 was verified by small angle XRD peak at 6.4°, which corresponds to the layer distance of 13. Read More

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Distinctive roles of fibrillar collagen I and collagen III in mediating fibroblast-matrix interaction: A nanoscopic study.

Biochem Biophys Res Commun 2021 May 8;560:66-71. Epub 2021 May 8.

Department of Chemistry, Illinois Institute of Technology, 3101 S. Dearborn St., Chicago, IL, 60616, USA. Electronic address:

One major goal in tissue engineering is to create functional materials, mimicking scaffolds in native tissues, to modulate cell function for tissue repair. Collagen is the most abundant structural protein in human body. Though collagen I (COLI) and collagen III (COLIII) are the predominant collagen types in connective tissues and they form stable hybrid fibrils at varied ratios, cell responses to the hybrid matrices are underinvestigated. Read More

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Development of a new method for isolation of urban air particulates deposited in the human lung tissue.

Chemosphere 2021 Apr 27;280:130585. Epub 2021 Apr 27.

Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran; Department of Research Methodology and Data Analysis, Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Particulate matters (PMs) are important pollutants in urban air pollution because of their variable composition. The pulmonary clearance of PMs is critical to prevent long-term immunological responses. This study established a new method for the isolation of probably deposited urban air particulates from the human lung tissue, to investigate the features of uncleared particulates. Read More

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Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organization.

Mutat Res 2021 Apr 20;822:111743. Epub 2021 Apr 20.

Department of Biology, University of Iowa, Iowa City, IA, 52241, USA. Electronic address:

DNA double-strand breaks (DSBs) are one of the most dangerous assaults on the genome, and yet their natural and programmed production are inherent to life. When DSBs arise close together they are particularly deleterious, and their repair may require an altered form of the DNA damage response. Our understanding of how clustered DSBs are repaired in the germline is unknown. Read More

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Ossifying low grade endometrial stromal sarcoma with PHF1-BRD8 fusion.

Cancer Genet 2021 Apr 22;256-257:81-85. Epub 2021 Apr 22.

Department of Pathology, Taipei Veterans General Hospital, No. 201, Sec. 2, Shih-Pai Road, Taipei 11217, Taiwan; Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.

Low-grade endometrial stromal sarcoma and ossifying fibromyxoid tumors are two types of mesenchymal tumors that share no similarities in terms of site, sex, and morphological characteristics. They are rare, low grade tumors of uncertain lineage, with no definite immunological markers. Interestingly, a common PHF1 gene- related rearrangement was observed in these two tumors. Read More

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Ultrastructural 3D reconstruction of the smallest known insect photoreceptors: The stemmata of a first instar larva of Strepsiptera (Hexapoda).

Arthropod Struct Dev 2021 May 8;62:101055. Epub 2021 May 8.

Entomology Group, Institut für Zoologie und Evolutionsforschung, Friedrich-Schiller-Universität Jena, Erbertstraße 1, 07743 Jena, Germany.

Stemmata of strepsipteran insects represent the smallest arthropod eyes known, having photoreceptors which form fused rhabdoms measuring an average size of 1.69 × 1.21 × 1. Read More

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Control of intestinal epithelial permeability by lysophosphatidic acid receptor 5.

Cell Mol Gastroenterol Hepatol 2021 May 8. Epub 2021 May 8.

Division of Digestive Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA; Gastroenterology Research, Atlanta Veterans Administration Medical Center, Decatur, GA, USA; Winship Cancer Institute, Emory University School of Medicine. Electronic address:

Background & Aims: Epithelial cells form a monolayer at mucosal surface that functions as a highly selective barrier. Lysophosphatidic acid (LPA) is a bioactive lipid that elicits a broad range of biological effects via cognate G protein-coupled receptors. LPA receptor 5 (LPA) is highly expressed in intestinal epithelial cells, but its role in the intestine is not well known. Read More

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Albumin conjugates of thiosemicarbazone and imidazole-2-thione prochelators: Iron coordination and antiproliferative activity.

ChemMedChem 2021 May 11. Epub 2021 May 11.

University of Arizona, Chemistry & Biochemistry, 1306 E. University Ave., 85721, Tucson, UNITED STATES.

The central role of iron in tumor progression and metastasis motivates the development of iron-binding approaches in cancer chemotherapy. Disulfide-based prochelators are reductively activated upon cellular uptake to liberate thiol chelators responsible for iron sequestration. Herein, a trimethyl thiosemicarbazone moiety and the imidazole-2-thione heterocycle are incorporated in this prochelator design. Read More

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Synergistic Engineering of Conduction Band, Conductivity, and Interface of Bilayered Electron Transport Layers with Scalable TiO and SnO Nanoparticles for High-Efficiency Stable Perovskite Solar Cells.

ACS Appl Mater Interfaces 2021 May 11. Epub 2021 May 11.

Department of Chemistry, National Central University, Jhong-Li, Chung-Li 32001, Taiwan.

A simple, synergistic engineering of the conduction band (CB), conductivity, and interface of TiO-based bilayered electron transport layers (ETLs) via scalable TiO and SnO nanoparticles processed at low temperature (≤ 100 °C) for regular planar perovskite solar cells (PSCs) was developed. The bottom layer (Lt-TiO:SnO nanocomposite film) was prepared by spin coating from the ethanol suspension of small ground TiO nanoparticles with big ground SnO nanoparticles as the additive. The top C-SnO layer (spin-coated from the concentrated commercial SnO nanoparticles (C-SnO NPs, 20 wt %, 7 nm in size suspended in HO)) can be regarded as an interlayer between Lt-TiO:SnO and perovskite (Psk) absorbers. Read More

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Differentiation and proliferation of spermatogonial stem cells using a three-dimensional decellularized testicular scaffold: a new method to study the testicular microenvironment in vitro.

Int Urol Nephrol 2021 May 11. Epub 2021 May 11.

Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, 1419433151, Tehran, Iran.

Purpose: Successful in vitro transplantation of spermatogonial stem cells (SSCs) demands effective culture systems for SSCs proliferation and differentiation. Natural extracellular matrix (ECM) creates a microenvironment suitable for culture of stem cells. In the present study, we intended to assess the capability of the porous scaffold consisting of hyaluronic acid (HA), chitosan, and decellularized testicular matrix (DTM) as a proper niche for SSCs seeding. Read More

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Danger-associated molecular pattern molecules and the receptor for advanced glycation end products enhance ANCA-induced responses.

Rheumatology (Oxford) 2021 May 11. Epub 2021 May 11.

Centre for Inflammatory Disease, Department of Immunology and Inflammation, Imperial College London, Hammersmith Hospital, London, UK.

Objectives: The pro-inflammatory activities of the calgranulins and HMGB1 can be counteracted by sRAGE the soluble form of their shared receptor. To understand the role of these molecules in AAV and their potential as therapeutic targets we have studied 1) The relationship between these DAMPS and disease activity, 2) The expression of RAGE and sRAGE in biopsy tissue and peripheral blood and 3) The effect of these molecules on ANCA-mediated cytokine production.

Methods: We examined circulating levels of calgranulins (S100A8/A9 and S100A12), HMGB1 and sRAGE by ELISA. Read More

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The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains.

Elife 2021 May 11;10. Epub 2021 May 11.

Department of Physiology, UT Southwestern Medical Center at Dallas, Dallas, United States.

Sigma 1 receptor (S1R) is a 223-amino-acid-long transmembrane endoplasmic reticulum (ER) protein. S1R modulates activity of multiple effector proteins and is a well-established drug target. However, signaling functions of S1R in cells are poorly understood. Read More

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Novel antimicrobial anionic cecropins from the spruce budworm feature a poly-L-aspartic acid C-terminus.

Proteins 2021 May 11. Epub 2021 May 11.

Institut de biologie intégrative et des systèmes (IBIS) and Faculté de médecine, Université Laval, Quebec City, Canada.

Cecropins form a family of amphipathic α-helical cationic peptides with broad-spectrum antibacterial properties and potent anticancer activity. The emergence of bacteria and cancer cells showing resistance to cationic antimicrobial peptides (CAMPs) has fostered a search for new, more selective and more effective alternatives to CAMPs. With this goal in mind, we looked for cecropin homologs in the genome and transcriptome of the spruce budworm, Choristoneura fumiferana. Read More

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Leptomeningeal and intraventricular myelomatosis manifesting an aggressive form of communicating hydrocephalus.

Neuropathology 2021 May 10. Epub 2021 May 10.

Department of Neuropathology, Institute of Brain Science, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.

Leptomeningeal myelomatosis (LMM) is a fatal complication that occurs in < 1% of patients with multiple myeloma. Many patients with LMM present with neurologic symptoms referable to cranial neuropathies, while the manifestation of communicating hydrocephalus has been underrecognized. A Japanese man with Bence Jones protein-κ multiple myeloma developed fever and headache at age 54 years. Read More

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Clinical and Laboratory Predictors of Severity, Criticality, and Mortality in COVID-19: A Multisystem Disease.

Adv Exp Med Biol 2021 ;1318:369-402

Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran.

Coronavirus disease 2019 (COVID-19) pandemic continues devastating effects on healthcare systems. Such a crisis calls for an urgent need to develop a risk stratification tool. The present chapter aimed to identify laboratory and clinical correlates of adverse outcomes in patients with COVID-19. Read More

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January 2021

How COVID-19 Has Globalized: Unknown Origin, Rapid Transmission, and the Immune System Nourishment.

Adv Exp Med Biol 2021 ;1318:119-147

Research Center for Immunodeficiencies, Children's Medical Center Hospital, Tehran University of Medical Sciences, Tehran, Iran.

The novel coronavirus disease (COVID-19) profoundly influences T-cell immunity. The counts of total T cells and T-cell subsets, especially CD4+ and CD8+ T cells, are decreased in patients with COVID-19. Also, the function of these cells becomes less effective as the expression of immune inhibitory receptors, such as Tim3 and PD-1, increases over time during the disease. Read More

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January 2021

A hemagglutination-based, semi-quantitative test for point-of-care determination of SARS-CoV-2 antibody levels.

medRxiv 2021 May 4. Epub 2021 May 4.

Serologic, point-of-care tests to detect antibodies against SARS-CoV-2 are an important tool in the COVID-19 pandemic. The majority of current point-of-care antibody tests developed for SARS-CoV-2 rely on lateral flow assays, but these do not offer quantitative information. To address this, we developed a new method of COVID-19 antibody testing employing hemagglutination tested on a dry card, similar to that which is already available for rapid typing of ABO blood groups. Read More

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A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis.

Nat Commun 2021 May 10;12(1):2610. Epub 2021 May 10.

Department of Medical Biochemistry, Amsterdam Cardiovascular Sciences, Amsterdam UMC, University of Amsterdam, location AMC, Amsterdam, The Netherlands.

Angiogenic sprouting relies on collective migration and coordinated rearrangements of endothelial leader and follower cells. VE-cadherin-based adherens junctions have emerged as key cell-cell contacts that transmit forces between cells and trigger signals during collective cell migration in angiogenesis. However, the underlying molecular mechanisms that govern these processes and their functional importance for vascular development still remain unknown. Read More

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Tumor Spheroids of an Aggressive Form of Central Neurocytoma Have Transit-Amplifying Progenitor Characteristics with Enhanced EGFR and Tumor Stem Cell Signaling.

Exp Neurobiol 2021 Apr;30(2):120-143

Department of Neurosurgery, Seoul National University College of Medicine, Seoul 03082, Korea.

Central neurocytoma (CN) has been known as a benign neuronal tumor. In rare cases, CN undergoes malignant transformation to glioblastomas (GBM). Here we examined its cellular origin by characterizing differentiation potential and gene expression of CN-spheroids. Read More

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Vps34 and TOR Kinases Coordinate mRNA Translation in the presence or absence of Ire1-dependent Splicing.

Mol Cell Biol 2021 May 10. Epub 2021 May 10.

Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI-53211, USA

In the budding yeast an mRNA, called , exists in a translationally repressed form in the cytoplasm. Under conditions of cellular stress, such as when unfolded proteins accumulate inside the endoplasmic reticulum (ER), an RNase Ire1 removes an intervening sequence (intron) from the mRNA by non-conventional cytosolic splicing. Removal of the intron results in translational de-repression of mRNA and production of a transcription factor that activates expressions of many enzymes and chaperones to increase the protein-folding capacity of the cell. Read More

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Smac mimetics and TRAIL cooperate to induce MLKL-dependent necroptosis in Burkitt's lymphoma cell lines.

Neoplasia 2021 May 7;23(5):539-550. Epub 2021 May 7.

Institute for Experimental Cancer Research in Pediatrics, Goethe-University Frankfurt, Germany. Electronic address:

Burkitt's lymphoma (BL) is a highly aggressive form of B-cell non-Hodgkin's lymphoma. The clinical outcome in children with BL has improved over the last years but the prognosis for adults is still poor, highlighting the need for novel treatment strategies. Here, we report that the combinational treatment with the Smac mimetic BV6 and TRAIL triggers necroptosis in BL when caspases are blocked by zVAD. Read More

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Endolysosomal N-glycan processing is critical to attain the most active form of the enzyme acid alpha-glucosidase.

J Biol Chem 2021 May 7:100769. Epub 2021 May 7.

Amicus Therapeutics, Inc. Philadelphia, PA, USA. Electronic address:

Acid alpha-glucosidase (GAA) is a lysosomal glycogen-catabolizing enzyme, the deficiency of which leads to Pompe disease. Pompe disease can be treated with systemic recombinant human GAA (rhGAA) enzyme replacement therapy (ERT), but the current standard of care exhibits poor uptake in skeletal muscles, limiting its clinical efficacy. Further, it is unclear how the specific cellular processing steps of GAA post-delivery to lysosomes impact its efficacy. Read More

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Mitochondrial matrix proteases - quality control and beyond.

FEBS J 2021 May 10. Epub 2021 May 10.

Institute for Mitochondrial Diseases and Aging, Medical Faculty, University of Cologne, D-50931, Cologne, Germany.

To ensure correct function, mitochondria have developed several mechanisms of protein quality control (QC). Protein homeostasis highly relies on chaperones and proteases to maintain proper folding and remove damaged proteins that might otherwise form cell-toxic aggregates. Besides quality control, mitochondrial proteases modulate and regulate many essential functions, such as trafficking, processing, and activation of mitochondrial proteins, mitochondrial dynamics, mitophagy, and apoptosis. Read More

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Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging.

PLoS One 2021 10;16(5):e0240768. Epub 2021 May 10.

Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Göttingen, Germany.

Electron microscopy (EM) has been employed for decades to analyze cell structure. To also analyze the positions and functions of specific proteins, one typically relies on immuno-EM or on a correlation with fluorescence microscopy, in the form of correlated light and electron microscopy (CLEM). Nevertheless, neither of these procedures is able to also address the isotopic composition of cells. Read More

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A first-takes-all model of centriole copy number control based on cartwheel elongation.

PLoS Comput Biol 2021 May 10;17(5):e1008359. Epub 2021 May 10.

Instituto Gulbenkian de Ciência, Oeiras, Portugal.

How cells control the numbers of its subcellular components is a fundamental question in biology. Given that biosynthetic processes are fundamentally stochastic it is utterly puzzling that some structures display no copy number variation within a cell population. Centriole biogenesis, with each centriole being duplicated once and only once per cell cycle, stands out due to its remarkable fidelity. Read More

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Targeting nonapoptotic pathways with functionalized nanoparticles for cancer therapy: current and future perspectives.

Nanomedicine (Lond) 2021 May 10. Epub 2021 May 10.

Nanobiotech Lab, Kirori Mal College, University of Delhi, Delhi, 110007, India.

Apoptotic death evasion is a hallmark of cancer progression. In this context, past decades have witnessed cytotoxic agents targeting apoptosis. However, owing to cellular defects in the apoptotic machinery, tumors develop resistance to apoptosis-based cancer therapies. Read More

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3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting.

J Vis Exp 2021 Apr 21(170). Epub 2021 Apr 21.

Biomanufacturing Center, Dept. of Mechanical Engineering, Tsinghua University; Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing; Department of Mechanical Engineering, Drexel University;

Microcarriers are beads with a diameter of 60-250 µm and a large specific surface area, which are commonly used as carriers for large-scale cell cultures. Microcarrier culture technology has become one of the main techniques in cytological research and is commonly used in the field of large-scale cell expansion. Microcarriers have also been shown to play an increasingly important role in in vitro tissue engineering construction and clinical drug screening. Read More

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A possible way to prevent the progression of bone lesions in multiple myeloma via Src-homology-region-2-domain-containing-phosphatase-1 activation.

J Cell Biochem 2021 May 10. Epub 2021 May 10.

Department of Molecular Immunology and Inflammation, Research Institute National Center for Global Health and Medicine, Tokyo, Japan.

On the basis of our recent findings, in which multiple receptor-mediated mast cell functions are regulated via a common signaling cascade, we posit that the formation and functioning of osteoclasts are also controlled by a similar common mechanism. These cells are derived from the same granulocyte/monocyte progenitors and share multiple receptors except those that are cell-specific. In both types of cells, all known receptors reside in lipid rafts, form multiprotein complexes with recruited signaling molecules, and are internalized upon receptor engagement. Read More

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Interferon-induced degradation of the persistent hepatitis B virus cccDNA form depends on ISG20.

EMBO Rep 2021 May 9:e49568. Epub 2021 May 9.

Institute of Virology, School of Medicine, Technical University of Munich / Helmholtz Zentrum München, Munich, Germany.

Hepatitis B virus (HBV) persists by depositing a covalently closed circular DNA (cccDNA) in the nucleus of infected cells that cannot be targeted by available antivirals. Interferons can diminish HBV cccDNA via APOBEC3-mediated deamination. Here, we show that overexpression of APOBEC3A alone is not sufficient to reduce HBV cccDNA that requires additional treatment of cells with interferon indicating involvement of an interferon-stimulated gene (ISG) in cccDNA degradation. Read More

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Reduced A-to-I editing of endogenous Alu RNAs in lung after SARS-CoV-2 infection.

Curr Res Immunol 2021 30;2:52-59. Epub 2021 Apr 30.

Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37212, USA.

Due to potential severity of disease caused by SARS-CoV-2 infection, it is critical to understand both mechanisms of viral pathogenesis as well as diversity of host responses to infection. Reduced A-to-I editing of endogenous double-stranded RNAs (dsRNAs), as a result of inactivating mutations in , produces one form of Aicardi-Goutières Syndrome, with an immune response similar to an anti-viral response. By analyzing whole genome RNA sequencing data, we find reduced levels of A-to-I editing of endogenous Alu RNAs in normal human lung cells after infection by SARS-CoV-2 as well as in lung biopsies from patients with SARS-CoV-2 infections. Read More

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