178 results match your criteria fluorescent biochip


A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration.

Front Microbiol 2020 16;11:590736. Epub 2020 Dec 16.

Department of Molecular Evolution, Centro de Astrobiología (INTA-CSIC), Madrid, Spain.

Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially abundant on the surface of Mars. As some bacterial strains are capable of using perchlorate as an electron acceptor under anaerobic conditions, their detection is relevant for environmental monitoring on Earth as well as for the search for life on Mars. Read More

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December 2020

Localized fluorescent imaging of multiple proteins on individual extracellular vesicles using rolling circle amplification for cancer diagnosis.

J Extracell Vesicles 2020 Oct 11;10(1):e12025. Epub 2020 Nov 11.

Henan Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases School of Pharmaceutical Sciences Zhengzhou University Zhengzhou 450001 China.

Extracellular vesicles (EV) have attracted increasing attention as tumour biomarkers due to their unique biological property. However, conventional methods for EV analysis are mainly based on bulk measurements, which masks the EV-to-EV heterogeneity in tumour diagnosis and classification. Herein, a localized fluorescent imaging method (termed Digital Profiling of Proteins on Individual EV, DPPIE) was developed for analysis of multiple proteins on individual EV. Read More

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October 2020

Global Detection of Proteins by Label-Based Antibody Array.

Methods Mol Biol 2021 ;2237:45-53

RayBiotech Life, Peachtree Corners, GA, USA.

Because of narrow availability of antibody pairs and potential cross-reactivity between antibodies, the development of sandwich-based antibody arrays which need a pair of antibodies for each target has been restricted to higher density resulting in limited proteomic breadth of detection. Label-based array is one way to overcome this obstacle by directly labeling all targets in samples with fluorescent dyes such as Cy3 and Cy5. The labeled samples are then applied on the antibody array chip composed of capture antibodies. Read More

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Sandwich-Based Antibody Arrays for Protein Detection.

Methods Mol Biol 2021 ;2237:1-10

RayBiotech Life, Peachtree Corners, GA, USA.

Sandwich-based antibody arrays enable the detection of multiple proteins simultaneously, thus offering a time- and cost-effective alternative to single-plex platforms. The protein of interest is "sandwiched" between an antibody that captures it to the array and a second antibody that is used for detection. Here we describe a 1-day procedure to process samples, such as serum or cell lysates, with a quantitative sandwich-based antibody array on a glass substrate using fluorescence. Read More

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A pyrene-based ratiometric fluorescent probe with a large Stokes shift for selective detection of hydrogen peroxide in living cells.

J Pharm Anal 2020 Oct 4;10(5):490-497. Epub 2020 Aug 4.

Department of Chemistry and Center of Super-Diamond and Advanced Films, City University of Hong Kong, Hong Kong, China.

Hydrogen peroxide (HO) plays a significant role in regulating a variety of biological processes. Dysregulation of HO can lead to various diseases. Although numerous fluorescent imaging probes for HO have been reported, the development of HO ratiometric fluorescent probe with large Stokes shift remains rather limited. Read More

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October 2020

Sensitive Signal Amplifying a Diagnostic Biochip Based on a Biomimetic Periodic Nanostructure for Detecting Cancer Exosomes.

ACS Appl Mater Interfaces 2020 Jul 14;12(30):33473-33482. Epub 2020 Jul 14.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan Province 450001, China.

Tumor-derived exosomes are emerging noninvasive biomarker reservoirs that reflect biological information from their parental cells, especially specific markers, including proteins, DNA fragments and RNAs. Recently, analytical methods of tumor-derived exosomes have been increasing growth. However, developing a convenient signal amplification technique to improve the sensitivity of exosomes detection still remains a challenge. Read More

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A fluorescent molecular rotor probe for tracking plasma membranes and exosomes in living cells.

Chem Commun (Camb) 2020 Jul;56(60):8480-8483

Department of Chemistry and COSDAF (Centre of Super-Diamond and Advanced Films), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China. and Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, P. R. China.

A rotor-based probe MRMP-1 was designed and synthesized. MRMP-1 can bind to plasma membranes very quickly and stably with remarkable fluorescence enhancement. It can be used to monitor the dynamic changes in cell membranes in real-time under stimuli conditions. Read More

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High-throughput single-molecule imaging system using nanofabricated trenches and fluorescent DNA-binding proteins.

Biotechnol Bioeng 2020 06 6;117(6):1640-1648. Epub 2020 Apr 6.

School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.

DNA curtain is a high-throughput system, integrating a lipid bilayer, fluorescence imaging, and microfluidics to probe protein-DNA interactions in real-time and has provided in-depth understanding of DNA metabolism. Especially, the microfluidic platform of a DNA curtain is highly suitable for a biochip. In the DNA curtain, DNA molecules are aligned along chromium nanobarriers, which are fabricated on a slide surface, and visualized using an intercalating dye, YOYO-1. Read More

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A fluorescent plasmonic biochip assay for multiplex screening of diagnostic serum antibody targets in human Lyme disease.

PLoS One 2020 10;15(2):e0228772. Epub 2020 Feb 10.

College of Nanoscale Science & Engineering, State University of New York Polytechnic Institute, Albany, New York, United States of America.

Lyme disease (LD) diagnosis using the current two-tier algorithm is constrained by low sensitivity for early-stage infection and ambiguity in determining treatment response. We recently developed a protein microarray biochip that measures diagnostic serum antibody targets using grating-coupled fluorescent plasmonics (GC-FP) technology. This strategy requires microliters of blood serum to enable multiplexed biomarker screening on a compact surface and generates quantitative results that can be further processed for diagnostic scoring. Read More

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Multicenter prospective study on multivariant diagnostics of autoimmune bullous dermatoses using the BIOCHIP technology.

J Am Acad Dermatol 2020 Nov 28;83(5):1315-1322. Epub 2020 Jan 28.

Institute of Experimental Immunology, EUROIMMUN AG, Lübeck, Germany.

Background: The current standard in the serologic diagnosis of autoimmune bullous diseases (AIBD) is a multistep procedure sequentially applying different assays. In contrast, the BIOCHIP Mosaic technology combines multiple substrates for parallel analysis by indirect immunofluorescence.

Methods: Sera from 749 consecutive, prospectively recruited patients with direct immunofluorescence-positive AIBD from 13 international study centers were analyzed independently and blinded by using (1) a BIOCHIP Mosaic including primate esophagus, salt-split skin, rat bladder, monkey liver, monkey liver with serosa, recombinant BP180 NC16A, and gliadin GAF3X, as well as HEK293 cells expressing recombinant desmoglein 1, desmoglein 3, type VII collagen, and BP230 C-terminus and (2) the conventional multistep approach of the Department of Dermatology, University of Lübeck. Read More

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November 2020

Performance evaluation of an indirect immunofluorescence kit for the serological diagnosis of dengue.

Einstein (Sao Paulo) 2020 20;18:eAO5078. Epub 2019 Dec 20.

Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.

Objective: To evaluate the performance of indirect immunofluorescence for serological diagnosis of dengue virus in a population with high prevalence of arboviruses.

Methods: Two-hundred serum samples from patients with clinical suspicion of dengue fever were tested by immunoenzymatic and indirect immunofluorescence assay BIOCHIP® mosaic. Specificity, sensitivity and Kappa coefficient were calculated. Read More

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Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum.

PLoS One 2019 2;14(12):e0225525. Epub 2019 Dec 2.

Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee, Freiburg, Germany.

The Systemic Inflammatory Response Syndrome (SIRS), a sepsis related inflammatory state, is a self-defense mechanism against specific and nonspecific stimuli. The six most extensively studied inflammatory biomarkers for the clinical diagnosis of SIRS are interleukin 4 (hIL-4), interleukin 6 (hIL-6), interleukin 10 (hIL-10), tumor necrosis factor alpha (hTNF-α), interferon gamma (hIFN-γ) and procalcitonin (hPCT). These biomarkers are naturally present (but usually only at low concentration) in SIRS infected patients [1, 2] and thus the development of a highly sensitive detection method is of major clinical interest. Read More

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The use of BIOCHIP mosaics in diagnostics of bullous pemphigoid: Evaluation and comparison to conventional multistep procedures.

J Cutan Pathol 2020 Feb 29;47(2):121-127. Epub 2019 Oct 29.

Department of Dermatology and Immunodermatology, Medical University of Warsaw, Warsaw, Poland.

Background: Bullous pemphigoid (BP) is an autoimmune blistering disease associated with autoantibodies against BP180 and/or BP230 antigens. The immunoassays available for serological diagnostics include indirect immunofluorescence (IIF) on monkey esophagus (ME), salt-split skin (SSS), and enzyme-linked immunosorbent assay (ELISA) for BP180-NC16a and BP230. Only a few studies validated innovative BIOCHIP mosaic, but none compared agreement between BIOCHIP substrates with conventional methods separately. Read More

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February 2020

A fluorogenic HS-triggered prodrug based on thiolysis of the NBD amine.

Bioorg Med Chem Lett 2019 10 17;29(19):126627. Epub 2019 Aug 17.

State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Engineering Research Center of Pesticide (Tianjin), College of Chemistry, Nankai University, Tianjin 300071, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), China. Electronic address:

Based on thiolysis of the NBD amine, a HS-triggered prodrug has been designed and synthesized for localized production of ciprofloxacin under micromolar HS. Activation of the prodrug can be monitored through fluorescence in real-time. We envision that thiolysis of the NBD amine could be readily used for development of other HS-triggered prodrugs in the future. Read More

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October 2019

An ultra-sensitive ratiometric fluorescent probe for hypochlorous acid detection by the synergistic effect of AIE and TBET and its application of detecting exogenous/endogenous HOCl in living cells.

J Mater Chem B 2019 09 31;7(33):5125-5131. Epub 2019 Jul 31.

Department of Chemistry and Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China. and Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, China.

An ultra-sensitive and ratiometric fluorescent probe for hypochlorous acid (HOCl) detection based on the mechanism of aggregation induced emission (AIE) and through-bond energy transfer (TBET) has been reported herein. By exploiting the advantages of AIE and TBET, which eliminates emission leakage from dark donors, the probe exhibits ultra-high sensitivity towards HOCl by an enhancement of over 7000-fold in the fluorescence ratio (I/I), which is one of the highest recorded so far. The reaction mechanism has been discussed in detail, and the effects of interferents and the reaction kinetics have also been investigated. Read More

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September 2019

Inter-rater reliability of the BIOCHIP indirect immunofluorescence dermatology mosaic in bullous pemphigoid and pemphigus patients.

J Eur Acad Dermatol Venereol 2019 Dec 12;33(12):2327-2333. Epub 2019 Aug 12.

University of New South Wales, Kogarah, NSW, Australia.

Background: The BIOCHIP (Dermatology Mosaic 7; EUROIMMUN, Lubeck, Germany) is a novel multiplex indirect immunofluorescence (IIF) technique used in the serological diagnosis of bullous pemphigoid (BP) and pemphigus.

Objective: To validate the accuracy and inter-rater reliability (IRR) of the BIOCHIP in the diagnosis of BP, pemphigus foliaceus (PF) and pemphigus vulgaris (PV).

Methods: Sera from patients with BP (n = 38), PF (n = 8), PV (n = 23), control patients (n = 64) and healthy control volunteers (n = 39) were tested. Read More

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December 2019

Validation of the BIOCHIP test for the diagnosis of bullous pemphigoid, pemphigus vulgaris and pemphigus foliaceus.

J Eur Acad Dermatol Venereol 2020 Jan 30;34(1):153-160. Epub 2019 Jul 30.

Department of Dermatology, St George Hospital, Sydney, NSW, Australia.

Background: The BIOCHIP is a novel multiplex indirect immunofluorescence technique used in the serological diagnosis of bullous pemphigoid and pemphigus. The BIOCHIP method combines the screening of autoantibodies and target antigen-specific substrates in a single miniature incubation field.

Objective: To evaluate the diagnostic accuracy of the new immunofluorescence BIOCHIP multiplex tool in pemphigus and bullous pemphigoid. Read More

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January 2020

Role of BIOCHIP Indirect Immunofluorescence Test in Cutaneous Vesiculobullous Diseases.

Am J Dermatopathol 2020 May;42(5):322-328

Department of Pathology, St. John's Medical College, Bengaluru, India.

BIOCHIP mosaics for indirect immunofluorescence in cutaneous vesiculobullous diseases provide antibody profiles in a single run and can be an alternative to performing multistep assays. There is scanty data regarding their utility. BIOCHIP tests performed over 4 years were compared with biopsy and/or direct immunofluorescence (DIF). Read More

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Label-free detection of γ-aminobutyric acid based on silicon nanowire biosensor.

Nano Converg 2019 May 1;6(1):13. Epub 2019 May 1.

Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 04107, Korea.

γ-Aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the central nervous system (CNS), which acts as a major biomarker for neurological disorders such as Parkinson's disease and Meningitis. To this end, the precise measurement of GABA molecule arisen as an important subject for the effective diagnosis and treatment of neurological disorders. However, yet highly sensitive biosensor systems which can analyze a wide range of GABA molecule in a fast response manner have not been reported. Read More

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A new indirect immunofluorescence BIOCHIP method for the serological diagnosis of bullous pemphigoid: A review of literature.

Australas J Dermatol 2019 Aug 9;60(3):e173-e177. Epub 2019 Apr 9.

Department of Dermatology, St George Hospital, Sydney, New South Wales, Australia.

The BIOCHIP (Dermatology Mosaic 7, EUROIMMUN, Lubeck, Germany) is a novel multiplex indirect immunofluorescence technique used in the serological diagnosis of bullous pemphigoid. The BIOCHIP method combines the screening of several autoantibodies and target antigen-specific substrates in a single miniature incubation field to allow for simultaneous processing of the most common autoimmune bullous diseases autoantibodies using a single investigation. This manuscript reviews the literature on the validity of the BIOCHIP in the diagnosis of bullous pemphigoid. Read More

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The use of BIOCHIP technique in diagnosis of different types of pemphigus: Vulgaris and foliaceus.

J Immunol Methods 2019 05 22;468:35-39. Epub 2019 Mar 22.

Department of Dermatology and Immunodermatology, Medical University of Warsaw, Poland.

Background: Pemphigus is a rare, autoimmune blistering disease characterized by autoantibodies against desmoglein 3 (Dsg3) and 1 (Dsg1) with mucosal and/or skin involvement. Main types of pemphigus include mucosal pemphigus vulgaris (m-PV), mucocutaneus pemphigus vulgaris (mc-PV) and pemphigus foliaceus (PF) determined by clinical picture, positive direct and indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA).

Methods: We evaluated the sensitivity and specificity of a novel multi-substrate immunofluorescence technique called BIOCHIP in the diagnosis of main types of pemphigus. Read More

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Photochemistry on the Space Station-Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station.

Astrobiology 2019 08 28;19(8):1063-1074. Epub 2019 Feb 28.

2Laboratoire d'Astrophysique de Bordeaux (LAB), Université de Bordeaux, CNRS, B18N, Pessac, France.

Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. Read More

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[Possibilities of cytological diagnosis of the nature of the exudate at the stage of emergency.]

Klin Lab Diagn 2018;63(12):768-772

Federal budgetary institution of science "Nizhny Novgorod research Institute of epidemiology and microbiology. Academician I.N. Blokhina Rospotrebnadzor", 603005, 603006, Nizhny Novgorod, Russia.

Cytological diagnosis by effusions is currently the only reliable method of morphological verification of the diagnosis, it has prognostic significance and determines the choice of treatment strategy. At the same time, the variability of normal mesothelial cells causes significant difficulties in its differential diagnosis with reactive mesothelium, malignant mesothelioma and cancer metastasis, which requires additional analytical methods. A retrospective study of cytological preparations for 2017 was conducted, as well as the effectiveness of the use of fluorescent immunocytochemistry (FITZ) on the test system "biochip" in combination with a traditional cytological study was evaluated. Read More

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Biochip detection of BP180 autoantibodies in blister fluid for the serodiagnosis of bullous pemphigoid: A pilot study.

Medicine (Baltimore) 2019 Feb;98(7):e14514

Dermatology Unit, Department of Medicine, University of Padua, Via Vincenzo Gallucci 4, Padua, Italy.

Bullous pemphigoid is an autoimmune subepidermal blistering skin disease immunologically defined by autoantibodies directed against basement membrane zone antigens, the main of which is BP180. Laboratory tests are essential for diagnosis and include direct immunofluorescence and serologic assessments with indirect immunofluorescence and ELISA. Serology may be performed on blister fluid, in alternative to blood serum. Read More

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February 2019

Novel dual-color drug screening model for GLUT4 translocation in adipocytes.

Mol Cell Probes 2019 02 11;43:6-12. Epub 2019 Jan 11.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China; The General Hospital of Ningxia Medical University, Department of Beijing National Biochip Research Center Sub-Center in Ningxia, Yinchuan, 750004, China. Electronic address:

Insulin-responsive glucose transporter type 4 (GLUT4) translocation plays a major role in controlling glucose uptake in adipose tissue and muscle, maintaining homeostasis and preventing hyperglycemia. Screening for chemicals enhancing GLUT4 translocation is an approach for identifying hits of drug development for type 2 diabetes. Here we developed a novel functional dual-color probe, pHluorin-GLUT4-mOrange2, and constructed 3T3-L1 adipocytes based screening system to simply and efficiently screen new compounds stimulating GLUT4 translocation. Read More

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February 2019

[Multiplex Assay to Evaluate the Genetic Risk of Developing Human Melanoma].

Mol Biol (Mosk) 2018 Nov-Dec;52(6):997-1005

Engelhardt Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 119991 Russia.

A genotyping procedure based on single-step PCR and subsequent allele-specific hybridization on a hydrogel biochip was developed to address the polymorphisms of HERC2, OCA2, SLC24A4, SLC45A2, TYR, IRF4, MC1R,MITF, PIGU, MYH7B, NCOA6, and CDK10. Amplified gene fragments were fluorescently labeled in PCR, and fluorescent signals from biochip cells were detected to evaluate how efficiently the PCR product formed a perfect duplex with an immobilized probe. The analytical characteristics of hybridization analysis were estimated for several fluorophores with different optical spectra. Read More

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[Overexpression of miR-451a inhibits cell proliferation by targeted macrophage migration inhibitory factor in HepG2 cells].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2018 Dec;34(12):1091-1098

Research Equipment Management Center, General Hospital of Ningxia Medical University, Yinchuan 750000, China. *Corresponding author, E-mail:

Objective To investigate the regulatory effect of miR-451a on macrophage migration inhibitory factor (MIF) and its effect on the proliferation of hepatocellular carcinoma HepG2 cells. Methods Real-time quantitative PCR was utilized to detect the expression of miR-451a and MIF mRNA in clinical liver cancer tissues. The luciferase reporter system was used to validate the regulatory relationship between miR-451a and MIF. Read More

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December 2018

A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV.

Biochip J 2019 11;13(4):341-351. Epub 2019 Nov 11.

6Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, South Korea.

The current diagnosis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) relies on laboratory-based tests since its clinical features are nonspecific, unlike other respiratory pathogens. Therefore, the development of a rapid and simple method for on-site detection of SARS-CoV is crucial for the identification and prevention of future SARS outbreaks. In this study, a simple colorimetric and multiplex loop-mediated isothermal amplification (LAMP) assay was developed to rapid screening of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). Read More

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November 2019

Femtomolar Detection of Lipopolysaccharide in Injectables and Serum Samples Using Aptamer-Coupled Reduced Graphene Oxide in a Continuous Injection-Electrostacking Biochip.

Anal Chem 2019 02 8;91(3):2360-2367. Epub 2019 Jan 8.

Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences , Southern Medical University , Guangzhou 510515 , China.

A method for microfluidic sample preconcentration to detect femtomolar level of lipopolysaccharide (LPS) is introduced, enabled by 6-carboxyfluorescein (6-FAM) labeled aptamer-LPS binding along with reduced graphene oxide (rGO). The free FAM-aptamers can be adsorbed onto the surface of rGO, resulting in fluorescence quenching of background signals. Conversely, the aptamer-LPS complex cannot be adsorbed by rGO, so the fluorescence is maintained and detected. Read More

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February 2019

An ultra-sensitive and ratiometric fluorescent probe based on the DTBET process for Hg detection and imaging applications.

Analyst 2019 Feb 19;144(4):1353-1360. Epub 2018 Dec 19.

Department of Materials Science and Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.

In this article, we present an ultra-sensitive and ratiometric fluorescent probe (TR-Hg) for Hg detection based on the mechanism of aggregation induced emission (AIE) and dark through-bond energy transfer (DTBET). The probe was constructed using tetraphenylethene as the dark donor and rhodamine B thiolactone as the acceptor. By exploiting the advantages of DTBET, which eliminates emission leakage from dark donors and provides nearly 100% energy transfer efficiency, TR-Hg exhibits more than a 30 000-fold fluorescence ratio enhancement after reacting with Hg. Read More

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February 2019