554,779 results match your criteria fluid volume

Cytomorphological spectrum of cysticercosis: a study of 26 cases.

Cytopathology 2021 Jul 31. Epub 2021 Jul 31.

Department Of Pathology, Atal Bihari Vajpayi Institute Of Medical Sciences & Dr. Ram Manohar Lohia Hospital, New Delhi, India.

Background: Cysticercosis is a systemic parasitic disease caused by the larval stage of Taenia solium. It is the most common parasitic disease worldwide. Fine needle aspiration cytology plays an important role in prompt recognition of this disease. Read More

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Autoantibody against the Rab6A/Rab6B in primary autoimmune cerebellar ataxia associated with Sjogren's syndrome: A case report.

J Neuroimmunol 2021 Jul 22;359:577667. Epub 2021 Jul 22.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

In the current study we report a novel autoantibody against Purkinje cells in a patient with primary autoimmune cerebellar ataxia (PACA) associated with Sjogren's syndrome (SS). Tissue-based indirect immunofluorescence assay (TBA) of the patient's serum and cerebrospinal fluid (CSF) revealed IgG antibody to Purkinje cells and the granular layer of the rat cerebellum. Rab6A was identified as autoantigen by mass spectrometry (MS) and Western blotting, and the interactions between Rab6A or its homologous Rab6B and autoantibody in patient serum were verified by recombinant cell-based assay (CBA) and neutralization experiments. Read More

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A fluid-supported 3D hydrogel bioprinting method.

Biomaterials 2021 Jul 21;276:121034. Epub 2021 Jul 21.

Molecular Engineering Laboratory, Institute of Molecular and Cell Biology, A*STAR, 61 Biopolis Drive, #03-13 Proteos, 138673, Singapore.

Hydrogels are used in many biomedical applications, including regenerative medicine and surgical training phantoms. However, the ability to shape these materials into complex anatomical structures using additive manufacturing is limited in part by their low mechanical stiffness. We developed a hydrogel 3D printer, that projects patterns directly onto a thin layer of fluid-supported hydrogel precursor, which serves as a floating, liquid projection screen. Read More

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Influence of the geometry of extra column volumes on band broadening in a chromatographic system. Predictions by computational fluid dynamics.

J Chromatogr A 2021 Jul 14;1653:462410. Epub 2021 Jul 14.

Department of Chemical and Process Engineering, Rzeszów University of Technology, Rzeszów, Poland. Electronic address:

A computational fluid dynamics method was used for prediction of flow behavior and band profiles of small- and macro-molecule compounds eluting in extra-column volumes (ECV) of an Äkta chromatographic system. The model compounds were: acetone, bovine serum albumin and an antibody. The construction of ECV was approximated by different types of geometries, starting from the simplest two-dimensional (2D) arrangement consisting of a straight capillary tube, and ending with a three-dimensional system (3D), which accounted for the flow path curvature of individual elements of ECV, including: injection loop capillary, multi-way valve, connecting capillary and detector cell. Read More

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An intelligent method based on feed-forward artificial neural network and least square support vector machine for the simultaneous spectrophotometric estimation of anti hepatitis C virus drugs in pharmaceutical formulation and biological fluid.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 22;263:120190. Epub 2021 Jul 22.

Department of Chemistry, North Tehran Branch, Islamic Azad University, Tehran, Iran.

This study proposed simple and reliable spectrophotometry method for simultaneous analysis of hepatitis C antiviral binary mixture containing sofosbuvir (SOF) and daclatasvir (DAC). This technique is based on the use of feed-forward artificial neural network (FF-ANN) and least square support vector machine (LS-SVM). FF-NN with Levenberg-Marquardt (LM) and Cartesian genetic programming (CGP) algorithms was trained to determine the best number of hidden layers and the number of neurons. Read More

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Human tear fluid modulates the Pseudomonas aeruginosa transcriptome to alter antibiotic susceptibility.

Ocul Surf 2021 Jul 28. Epub 2021 Jul 28.

School of Optometry, University of California, Berkeley, CA, USA; Graduate Groups in Vision Science, Microbiology, and Infectious Diseases & Immunity, University of California, Berkeley, CA, USA. Electronic address:

Purpose: Previously, we showed that tear fluid protects corneal epithelial cells against Pseudomonas aeruginosa without suppressing bacterial viability. Here, we studied how tear fluid affects bacterial gene expression.

Methods: RNA-sequencing was used to study the P. Read More

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Standard therapy of Mycobacterium avium complex pulmonary disease shows limited efficacy in an open source hollow fiber system that simulates human plasma and epithelial lining fluid pharmacokinetics.

Clin Microbiol Infect 2021 Jul 28. Epub 2021 Jul 28.

Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands.

Objectives: Mycobacterium avium complex (MAC) bacteria can cause chronic pulmonary disease (PD). Current treatment regimens of azithromycin, ethambutol and rifampicin have culture conversion rates of around 65%. Dynamic, pre-clinical models to assess the efficacy of treatment regimens are important to guide clinical trial development. Read More

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Strategies for Multivariate Analyses of Imaging Genetics Study in Alzheimer's disease.

Neurosci Lett 2021 Jul 28:136147. Epub 2021 Jul 28.

School of Computer Science and Technology, Hangzhou Dianzi University, Hangzhou, Zhejiang, 310018, China; Key Laboratory of Intelligent Image Analysis for Sensory and Cognitive Health, Ministry of Industry and Information Technology of China, Hangzhou, Zhejiang, 310018, China.

Alzheimer's disease (AD) is an incurable neurodegenerative disease primarily affecting the elderly population. Early diagnosis of AD is critical for the management of this disease. Imaging genetics examines the influence of genetic variants (i. Read More

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Ripple-like instability in the simulated gel phase of finite size phosphocholine bilayers.

Biochim Biophys Acta Biomembr 2021 Jul 28:183714. Epub 2021 Jul 28.

Institut Charles Sadron, CNRS and University of Strasbourg, 23 rue du Loess, F-67034 Strasbourg, France. Electronic address:

Atomistic molecular dynamics simulations have reached a degree of maturity that makes it possible to investigate the lipid polymorphism of model bilayers over a wide range of temperatures. However if both the fluid L and tilted gel [Formula: see text] states are routinely obtained, the [Formula: see text] ripple phase of phosphatidylcholine lipid bilayers is still unsatifactorily described. Performing simulations of lipid bilayers made of different numbers of DPPC (1,2-dipalmitoylphosphatidylcholine) molecules ranging from 32 to 512, we demonstrate that the tilted gel phase [Formula: see text] expected below the pretransition cannot be obtained for large systems (equal or larger than 94 DPPC molecules) through common simulations settings or temperature treatments. Read More

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Mathematical modeling of surface deformation during vitrification.

Cryobiology 2021 Jul 28. Epub 2021 Jul 28.

Biothermal Technology Laboratory, Department of Mechanical Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA, 15213, United States. Electronic address:

Mathematical modeling of surface deformation during cryopreservation by vitrification is presented in this study. The specific problem under consideration is of a cryoprotective agent (CPA) solution vitrifying in a vial, following previously obtained cryomacroscopy observations. A multiphysics solution is proposed in this study, combining coupled effects associated with heat transfer, fluid mechanics, and solid mechanics. Read More

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Regulation of male fertility and accessory gland gene expression by the Drosophila HR39 nuclear receptor.

Dev Biol 2021 Jul 28. Epub 2021 Jul 28.

Department of Human Genetics, University of Utah School of Medicine, 15 North 2030 East, Rm 5100, Salt Lake City, UT, 84112, USA. Electronic address:

Successful reproduction is dependent on the transfer of male seminal proteins to females upon mating. These proteins arise from secretory tissues in the male reproductive tract, including the prostate and seminal vesicles in mammals and the accessory gland in insects. Although detailed functional studies have provided important insights into the mechanisms by which accessory gland proteins support reproduction, much less is known about the molecular mechanisms that regulate their expression within this tissue. Read More

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3D migration of human amniotic fluid stem cells involves mesenchymal and amoeboid modes and is regulated by mTORC1.

Stem Cells 2021 Jul 31. Epub 2021 Jul 31.

Institute of Medical Genetics, Center of Pathobiochemistry and Genetics, Medical University of Vienna, Vienna, Austria.

3D cell migration is an integral part of many physiologic processes. Although being well studied in the context of adult tissue homeostasis and cancer development, remarkably little is known about the invasive behavior of human stem cells. Using two different kinds of invasion assays, this study aimed at investigating and characterizing the 3D migratory capacity of human amniotic fluid stem cells (hAFSCs), a well-established fetal stem cell type. Read More

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Hemodynamic factor evaluation using computational fluid dynamics analysis for de novo bleb formation in unruptured intracranial aneurysms.

Neurol Sci 2021 Jul 31. Epub 2021 Jul 31.

Department of Neurosurgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan.

Background: Although bleb formation increases the risk of rupture of intracranial aneurysms, previous computational fluid dynamic (CFD) studies have been unable to identify robust causative hemodynamic factors, due to the morphological differences of prebleb aneurysm models and a small number of aneurysms with de novo bleb formation. This study investigated the influences of differences in the aneurysm-models and identify causative hemodynamic factors for de novo bleb formation.

Materials And Methods: CFD analysis was conducted on three aneurysm models, actual prebleb, postbleb, and virtual prebleb models of two unruptured aneurysms with de novo bleb formation. Read More

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Predictive performance of dynamic arterial elastance for arterial pressure response to fluid expansion in mechanically ventilated hypotensive adults: a systematic review and meta-analysis of observational studies.

Ann Intensive Care 2021 Jul 31;11(1):119. Epub 2021 Jul 31.

Department of Intensive Care Medicine, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, 315000, Zhejiang, China.

Background: Dynamic arterial elastance (Ea) has been extensively considered as a functional parameter of arterial load. However, conflicting evidence has been obtained on the ability of Ea to predict mean arterial pressure (MAP) changes after fluid expansion. This meta-analysis sought to assess the predictive performance of Ea for the MAP response to fluid expansion in mechanically ventilated hypotensive patients. Read More

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Association of BRAF V600E mutations with vasoactive intestinal peptide syndrome in MYCN-amplified neuroblastoma.

Pediatr Blood Cancer 2021 Jul 31:e29265. Epub 2021 Jul 31.

Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, New York.

Very rarely, vasoactive intestinal peptide-related diarrhea (VIP-D) is observed in patients with high-risk neuroblastoma (HR-NB) where the associated fluid and electrolyte abnormalities can pose a major clinical challenge for administering the required aggressive multimodality treatment. Two patients with HR-NB developed VIP-D during induction and were found to have a somatic BRAF V600E mutation. Serum VIP levels and diarrhea promptly resolved in both patients after initiating treatment with BRAF and MEK inhibitors. Read More

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Current salivary biomarkers for detection of human papilloma virus-induced oropharyngeal squamous cell carcinoma.

Head Neck 2021 Jul 31. Epub 2021 Jul 31.

Department of Otolaryngology, University of Miami Miller School of Medicine, Miami, Florida, USA.

Human papilloma virus (HPV) infection is a key risk factor and etiology for oropharyngeal squamous cell carcinoma (OPSCC). HPV-induced OPSCC is rapidly increasing in incidence, with men experiencing increased mortality. When identified at an early stage, HPV-induced OPSCC can be successfully treated. Read More

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Validation of the Orlando Protocol for endoscopic management of pancreatic fluid collections in the era of lumen-apposing metal stents.

Dig Endosc 2021 Jul 31. Epub 2021 Jul 31.

Digestive Health Institute, Orlando Health, Orlando, Florida, USA.

Objectives: Although lumen-apposing metal stents (LAMS) are being increasingly used in lieu of plastic stents, clinical approach to endoscopic management of pancreatic fluid collections (PFCs) is poorly standardized. We compared outcomes of approaches over two time intervals, initially using plastic stents and later integrating LAMS.

Methods: This was a retrospective, observational, before-after study of prospectively collected data on consecutive patients with symptomatic PFCs managed over two time periods. Read More

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Isolation of Lymphatic Muscle Cells (LMCs ) from Rat Mesentery.

Methods Mol Biol 2021 ;2319:137-141

Department of Medical Physiology, College of Medicine, Texas A&M University, Bryan, TX, USA.

Lymphatic muscle cells (LMCs), with unique characteristics resembling a combination of both cardiac and smooth muscle cells, play an essential role in the spontaneous contraction of the lymphatic vessels to pump fluid forward. However, our understanding of the more detailed molecular phenotypes of LMCs is limited. Here, we described a method to isolate the LMCs from rat mesentery and then culture the cells in vitro, which will serve a lot more molecular biology study of LMCs and significantly improve our knowledge about the unique characteristics of LMCs. Read More

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January 2021

In Vitro Study of Permeability in Lymphatic Endothelial Cells Responding to Histamine.

Hongjiang Si

Methods Mol Biol 2021 ;2319:45-49

Department of Medical Physiology, College of Medicine, Texas A&M University, Bryan, TX, USA.

Histamine is well characterized to cause hyperpermeability in both blood and lymphatic endothelial cells (BECs and LECs) in infection and inflammation. The increased permeability impairs the barrier function of vessels to fluid, soluble electrolytes, and proteins, resulting in the swelling of interstitial tissues, termed edema and lymphedema. Here, we describe two approaches to study the permeability of LECs, to macromolecules or to electrolytes, upon histamine stimulation in vitro. Read More

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January 2021

RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells.

Methods Mol Biol 2021 ;2319:25-30

Department of Medical Physiology, College of Medicine, Texas A&M University, Bryan, TX, USA.

Fluid shear stress (FSS) is an important mechanical force that regulates endothelial and vascular functions in the blood and lymphatic vasculature. RNA-sequencing (RNA-Seq), an emerging next-generation sequencing (NGS) technique, facilitates us to quantify the global gene expression levels and, thereby, provides us with a powerful tool to study the transcriptional reprograming in the cells. Here, we describe the RNA-Seq procedures and the subsequent bioinformatic analysis to understand the transcriptome regulation by FSS in lymphatic endothelial cells (LECs). Read More

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January 2021

Dynamic Intracranial Pressure Waveform Morphology Predicts Ventriculitis.

Neurocrit Care 2021 Jul 30. Epub 2021 Jul 30.

Department of Neurology, Columbia University Vagelos College of Physicians and Surgeons, 177 Fort Washington Ave, 8 Milstein - 300 Center, New York, NY, USA.

Background: Intracranial pressure waveform morphology reflects compliance, which can be decreased by ventriculitis. We investigated whether morphologic analysis of intracranial pressure dynamics predicts the onset of ventriculitis.

Methods: Ventriculitis was defined as culture or Gram stain positive cerebrospinal fluid, warranting treatment. Read More

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Spared Nerve Injury Causes Sexually Dimorphic Mechanical Allodynia and Differential Gene Expression in Spinal Cords and Dorsal Root Ganglia in Rats.

Mol Neurobiol 2021 Jul 30. Epub 2021 Jul 30.

Department of Pharmacology, Faculty of Medicine, University of Helsinki, Haartmaninkatu 8 (Biomedicum 1), 00014, Helsinki, Finland.

Neuropathic pain is more prevalent in women. However, females are under-represented in animal experiments, and the mechanisms of sex differences remain inadequately understood. We used the spared nerve injury (SNI) model in rats to characterize sex differences in pain behaviour, unbiased RNA-Seq and proteomics to study the mechanisms. Read More

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Analysis of the main soft tissue stress associated with flexible flatfoot deformity: a finite element study.

Biomech Model Mechanobiol 2021 Jul 30. Epub 2021 Jul 30.

Department of Orthopedics, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, People's Republic of China.

A better understanding of soft tissue stress and its role in supporting the medial longitudinal arch in flexible flatfoot could help to guide the clinical treatment. In this study, a 3-Dimensional finite element (FE) foot model was reconstructed to measure the stress of the soft tissue, and its variation in different scenarios related to flexible flatfoot. All bones, cartilages, ligaments and related tendons around the ankle, and fat pad were included in the finite element model. Read More

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Non-invasive characterization of pleural and pericardial effusions using T1 mapping by magnetic resonance imaging.

Eur Heart J Cardiovasc Imaging 2021 Jul 30. Epub 2021 Jul 30.

Department of Cardiac Imaging, Barts Heart Centre, St Bartholomew's Hospital, King George V Building, West Smithfield, London EC1A 7BE, UK.

Aims: Differentiating exudative from transudative effusions is clinically important and is currently performed via biochemical analysis of invasively obtained samples using Light's criteria. Diagnostic performance is however limited. Biochemical composition can be measured with T1 mapping using cardiovascular magnetic resonance (CMR) and hence may offer diagnostic utility for assessment of effusions. Read More

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Validation of nasospheroids to assay CFTR functionality and modulator responses in cystic fibrosis.

Sci Rep 2021 Jul 30;11(1):15511. Epub 2021 Jul 30.

Medicine Genetics Group, Vall D'Hebron Research Institute, 08035, Barcelona, Spain.

The availability of a simple, robust and non-invasive in vitro airway model would be useful to study the functionality of the cystic fibrosis transmembrane regulator (CFTR) protein and to personalize modulator therapy for cystic fibrosis (CF) patients. Our aim was to validate a CFTR functional study using nasospheroids, a patient-derived nasal cell 3D-culture. We performed live-cell experiments in nasospheroids obtained from wild-type individuals and CF patients with different genotypes and phenotypes. Read More

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Quasi-equilibrium phase coexistence in single component supercritical fluids.

Nat Commun 2021 Jul 30;12(1):4630. Epub 2021 Jul 30.

Department of Physics, Pohang University of Science and Technology, Pohang, Republic of Korea.

In their supercritical state simple fluids are generally thought to assume a homogeneous phase throughout all combinations of pressures and temperatures, although various response functions or transport properties may exhibit anomalous behavior, characterizing a state point as either more gas-like or liquid-like, respectively. While a large body of results has been compiled in the last two decades regarding the details of the supercritical phase in thermodynamic equilibrium, far less studies have been dedicated to out-of-equilibrium situations that nevertheless occur along with the handling of substances such as carbon dioxide or Argon. Here we consider successive compression-expansion cycles of equal amounts of Argon injected into a high-pressure chamber, traversing the critical pressure at two times the critical temperature. Read More

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Multimodal single-cell omics analysis identifies epithelium-immune cell interactions and immune vulnerability associated with sex differences in COVID-19.

Signal Transduct Target Ther 2021 Jul 30;6(1):292. Epub 2021 Jul 30.

Genomic Medicine Institute, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.

Sex differences in the susceptibility of SARS-CoV-2 infection and severity have been controversial, and the underlying mechanisms of COVID-19 in a sex-specific manner remain understudied. Here we inspected sex differences in SARS-CoV-2 infection, hospitalization, admission to the intensive care unit (ICU), sera inflammatory biomarker profiling, and single-cell RNA-sequencing (scRNA-seq) profiles across nasal, bronchoalveolar lavage fluid (BALF), and peripheral blood mononuclear cells (PBMCs) from COVID-19 patients with varying degrees of disease severities. Our propensity score-matching observations revealed that male individuals have a 29% elevated likelihood of SARS-CoV-2 positivity, with a hazard ratio (HR) 1. Read More

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Clinical, laboratory and brain Magnetic Resonance Imaging (MRI) characteristics of asymptomatic and symptomatic HIV-negative neurosyphilis patients.

J Infect Chemother 2021 Jul 27. Epub 2021 Jul 27.

Department of Dermatology,Jinling Hospital, Nanjing Medical University, Nanjing, Jiangsu, 210002, China. Electronic address:

Introduction: There are few studies concerning the differences between asymptomatic neurosyphilis (ANS) and symptomatic neurosyphilis (SNS). This study aimed to summarize clinical, laboratory and brain Magnetic Resonance Imaging (MRI) characteristics of HIV-negative patients with ANS and SNS.

Methods: Data from 43 HIV-negative patients with ANS and 59 HIV-negative patients with SNS were retrospectively collected from our hospital between December 2012 and December 2018. Read More

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Evaluation of techniques used to assess skeletal muscle quantity in patients with cirrhosis.

Clin Nutr ESPEN 2021 Aug 11;44:287-296. Epub 2021 Jun 11.

School of Medicine, The University of Queensland, Brisbane, Queensland, Australia; Department of Gastroenterology and Hepatology, Princess Alexandra Hospital, Brisbane, Queensland, Australia; Translational Research Institute, Brisbane, Australia.

Introduction: Loss of skeletal muscle mass is a well-recognised complication of cirrhosis. Bedside methods to assess skeletal muscle mass including anthropometrics and bioelectrical impedance analysis (BIA) are negatively impacted by fluid overload in advanced cirrhosis and thus there is a need to identify alternatives. There is a paucity of data on the accuracy of commonly used radiological methods such as dual X-ray absorptiometry (DXA) to assess appendicular lean mass (ALM), and computed tomography (CT) skeletal muscle area in patients with cirrhosis. Read More

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A case of neovagina surgical creation using the uterine cervix remnant in a patient with Mayer-Rokitansky-Küster-Hauser syndrome.

Fertil Steril 2021 Jul 27. Epub 2021 Jul 27.

Limited Liability Company "Clinika Doktora Medvedeva", Dnipro, Ukraine.

Objective: To study the efficacy of a novel approach to vaginoplasty in a patient with vaginal aplasia and a preserved cervix.

Design: We present the case of a 28-year-old patient who was referred with a history of absent menstruation and vaginal intercourse was absent as well as abnormal development of the internal genitals. The patient underwent a laparoscopic cystectomy in 2012 because of an ovarian cyst and later that year underwent laparotomic supracervical hysterectomy with bilateral salpingectomy because of severe pain caused by a hematometra in their uterine remnant. Read More

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