165,580 results match your criteria fluid cell


Effect of the Fungal Immunomodulatory Protein FIP-fve in the Neutrophilic Asthma Animal Model.

Int Arch Allergy Immunol 2021 Oct 14:1-12. Epub 2021 Oct 14.

Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.

Background: Asthma animal models provide valuable information about the pathogenesis and the treatment of asthma. An ovalbumin (OVA)/complete Freund's adjuvant (CFA)-sensitized model was developed to induce neutrophil-dominant asthma and to investigate whether fungal immunomodulatory peptide-fve (FIP-fve) could improve asthma features in the OVA/CFA-sensitized model.

Methods: We used female BALB/c mice and sensitized them intraperitoneally with OVA/CFA on days 1, 2, and 3. Read More

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October 2021

Biofilm with highly heterogeneous interior structure for pollutant removal: Cell distribution and manipulated mass transport.

Bioresour Technol 2021 Sep 8;343:125913. Epub 2021 Sep 8.

Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan; Department of Mechanical Engineering, City University of Hong Kong, Kowloon Tang, Hong Kong, China; College of Engineering, Tunghai University, Taichung 40704, Taiwan. Electronic address:

Biofilm wastewater treatment had been applied in practice. Conventionally the biofilm was modeled as a uniform structure to simplify the analysis. This study for the first time established a three-dimensional biofilm model with distributions separating living cells, Extracellular polymeric substances (EPS) and pores, based on which the local fluid flow velocity and pollutant diffusion and reaction fluxes inside the biofilm were numerically evaluated. Read More

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September 2021

Glycine-Rich Peptides from FUS Have an Intrinsic Ability to Self-Assemble into Fibers and Networked Fibrils.

Biochemistry 2021 Oct 14. Epub 2021 Oct 14.

Department of Biomedical Engineering and Center for Science & Engineering of Living Systems (CSELS), Washington University in St. Louis, St. Louis, Missouri 63130, United States.

Glycine-rich regions feature prominently in intrinsically disordered regions (IDRs) of proteins that drive phase separation and the regulated formation of membraneless biomolecular condensates. Interestingly, the Gly-rich IDRs seldom feature poly-Gly tracts. The protein fused in sarcoma (FUS) is an exception. Read More

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October 2021

Tissue engineering with mechanically induced solid-fluid transitions.

Adv Mater 2021 Oct 14:e2106149. Epub 2021 Oct 14.

Institute of Mechanical Engineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, 1015, Switzerland.

Epithelia are contiguous sheets of cells that stabilize the shape of internal organs and support their structure by covering their surfaces. They acquire diverse morphological forms appropriate for their specific functions during embryonic development, such as the kidney tubules and the complex branching structures found in the lung. The maintenance of epithelial morphogenesis and homeostasis is controlled by their remarkable mechanics-epithelia can become elastic, plastic, and viscous by actively remodelling cell-cell junctions and modulating the distribution of local stresses. Read More

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October 2021

Biological and Psychological Factors Determining Neuropsychiatric Outcomes in COVID-19.

Curr Psychiatry Rep 2021 Oct 1;23(10):68. Epub 2021 Oct 1.

Mood and Anxiety Program, Department of Psychiatry, University of Maryland School of Medicine, 685 W. Baltimore Street, Suite# 930, Baltimore, MD, USA.

Purpose Of Review: We present biological and psychological factors implicated in psychiatric manifestations of SARS-CoV-2, as well as its neuroinvasive capability and immune pathophysiology.

Recent Findings: Preexisting mental illness leads to worse clinical outcomes in COVID-19. The presence of the virus was reported in the cerebrospinal fluid (CSF) and brain tissue post-mortem. Read More

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October 2021

Severe cisplatin-induced renal toxicity in a patient with xeroderma pigmentosum.

J Oncol Pharm Pract 2021 Oct 14:10781552211038246. Epub 2021 Oct 14.

Cancer Care Services, Toowoomba Hospital, Australia.

Introduction: Xeroderma pigmentosum is a rare genetic disorder of DNA repair, defined by extreme sensitivity to sunlight, leading to sunburn, skin pigmentation and increased incidence of skin cancers. Cisplatin acts by interfering with DNA repair mechanisms to cause DNA damage and apoptosis. It has indications in many malignancies including bladder, head and neck and lung cancers. Read More

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October 2021

Significant blunt bowel and mesenteric injury - Comparison of two CT scoring systems in a trauma registry cohort.

Eur J Radiol Open 2021 30;8:100380. Epub 2021 Sep 30.

Department of Diagnostic and Interventional Radiology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.

Purpose: This study evaluated and compared two imaging-based scoring systems for the detection of significant blunt bowel and mesenteric injury (sBBMI) by emergency computed tomography (CT).

Methods And Materials: We included all consecutive adult polytrauma patients admitted to our emergency department following a road traffic accident from January 2008 to June 2015, provided that intravenously contrast-enhanced whole-body CT examination was performed immediately after hospital admission. Two radiologists, blinded to patients' outcome, reviewed the CT examinations for distinctive direct intestinal or mesenteric vascular injury and indirect signs of abdominal injury. Read More

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September 2021

Neutrophil-derived heparin binding protein triggers vascular leakage and synergizes with myeloperoxidase at the early stage of severe burns (With video).

Burns Trauma 2021 17;9:tkab030. Epub 2021 Sep 17.

Department of Burns and Plastic Surgery, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China.

Background: Burn shock caused by vascular leakage is one of the main causes of high mortality in severe burn injury. However, the pathophysiological mechanism of vascular leakage is still unclear. The purpose of this study was to explore the molecular mechanism of vascular leakage in the early stage of severe burn and provide a new target for the treatment of severe burns. Read More

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September 2021

Therapeutic Potential of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Recovering From Murine Pulmonary Emphysema Under Cigarette Smoke Exposure.

Front Med (Lausanne) 2021 27;8:713824. Epub 2021 Sep 27.

School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan.

Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were shown to have potential for immunoregulation and tissue repair. The objective of this study was to investigate the effects of hUC-MSCs on emphysema in chronic obstructive pulmonary disease (COPD). The C57BL/6JNarl mice were exposed to cigarette smoke (CS) for 4 months followed by administration of hUC-MSCs at 3 × 10 (low dose), 1 × 10 (medium dose), and 3 × 10 cells/kg body weight (high dose). Read More

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September 2021

Hybrid Nanofluids as Renewable and Sustainable Colloidal Suspensions for Potential Photovoltaic/Thermal and Solar Energy Applications.

Front Chem 2021 27;9:737033. Epub 2021 Sep 27.

Department of Physics, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia.

The comparative utilization of solar thermal or photovoltaic systems has significantly increased to fulfill the requirement of electricity and heat since few decades. These hybrid systems produce both thermal and electrical energy simultaneously. In recent times, increasing interest is being redirected by researchers in exploiting variety of nanoparticles mixed with miscellaneous base fluids (hybrid nanofluid) for these hybrid systems. Read More

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September 2021

Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles.

Front Chem 2021 27;9:734280. Epub 2021 Sep 27.

Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, United States.

Expressed prostatic secretions (EPS), also called post digital rectal exam urines, are proximal fluids of the prostate that are widely used for diagnostic and prognostic assays for prostate cancer. These fluids contain an abundant number of glycoproteins and extracellular vesicles secreted by the prostate gland, and the ability to detect changes in their N-glycans composition as a reflection of disease state represents potential new biomarker candidates. Methods to characterize these N-glycan constituents directly from clinical samples in a timely manner and with minimal sample processing requirements are not currently available. Read More

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September 2021

Potential Molecular Biomarkers of Vestibular Schwannoma Growth: Progress and Prospects.

Front Oncol 2021 27;11:731441. Epub 2021 Sep 27.

Department of Pharmacy, Huashan Hospital, Fudan University, Shanghai, China.

Vestibular schwannomas (VSs, also known as acoustic neuromas) are relatively rare benign brain tumors stem from the Schwann cells of the eighth cranial nerve. Tumor growth is the paramount factor for neurosurgeons to decide whether to choose aggressive treatment approach or careful follow-up with regular magnetic resonance imaging (MRI), as surgery and radiation can introduce significant trauma and affect neurological function, while tumor enlargement during long-term follow-up will compress the adjacent nerves and tissues, causing progressive hearing loss, tinnitus and vertigo. Recently, with the deepening research of VS biology, some proteins that regulate merlin conformation changes, inflammatory cytokines, miRNAs, tissue proteins and cerebrospinal fluid (CSF) components have been proposed to be closely related to tumor volume increase. Read More

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September 2021

Bone Metastases of Glioblastoma: A Case Report and Review of the Literature.

Front Oncol 2021 27;11:705455. Epub 2021 Sep 27.

Department of Radiation Oncology, Weifang People's Hospital, Weifang, China.

Background: Glioblastoma (GBM) is the most common primary intracranial tumor and originates from the small pool of adult neural stem and progenitor cells (NSPCs). According to the World Health Organization (WHO) classification of brain tumors, gliomas are classified into grades I-IV, and GBM is defined as the highest grade (IV). GBM can be disseminated by cerebrospinal fluid (CSF), but extracranial metastasis is rare. Read More

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September 2021

Neurofilament Proteins as Biomarkers to Monitor Neurological Diseases and the Efficacy of Therapies.

Front Neurosci 2021 27;15:689938. Epub 2021 Sep 27.

Center for Dementia Research, Nathan Kline Institute, Orangeburg, NY, United States.

Biomarkers of neurodegeneration and neuronal injury have the potential to improve diagnostic accuracy, disease monitoring, prognosis, and measure treatment efficacy. Neurofilament proteins (NfPs) are well suited as biomarkers in these contexts because they are major neuron-specific components that maintain structural integrity and are sensitive to neurodegeneration and neuronal injury across a wide range of neurologic diseases. Low levels of NfPs are constantly released from neurons into the extracellular space and ultimately reach the cerebrospinal fluid (CSF) and blood under physiological conditions throughout normal brain development, maturation, and aging. Read More

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September 2021

Extra-cellular vesicles of the male genital tract: new actors in male fertility?

Basic Clin Androl 2021 Oct 14;31(1):25. Epub 2021 Oct 14.

CHU Rennes, Service de Biologie de la Reproduction-CECOS, 35000, Rennes, France.

Extracellular Vesicles (EVs) are membrane-limited particles containing proteins, lipids, metabolites and nucleic acids that are secreted by healthy and cancerous cells. These vesicles are very heterogeneous in size and content and mediate a variety of biological functions. Three subtypes of EV have been described in the male genital tract: microvesicles, myelinosomes and exosomes. Read More

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October 2021

Models in vivo of wound healing in the horse and the role of growth factors.

Vet Dermatol 1997 Dec;8(4):259-272

University of Liverpool, Equine studies division, Department of Veterinary Clinical Science, Leahurst, Neston, South Wirral, Merseyside L64 7TE, UK.

Abstract  Wound models attempt to simulate the natural healing processes in wounds. However, all models have significant limitations due to the complexity of the tissue repair process. Much can be learned from wound models in vitro by the use of cell culture techniques. Read More

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December 1997

Clinical Features and Outcomes of Streptococcus pneumoniae Meningitis in Children: A Retrospective Analysis of 26 Cases in China.

Neuropediatrics 2021 Oct 13. Epub 2021 Oct 13.

Department of Neurology, Children's Hospital of Shanxi, Taiyuan, Shanxi, China.

Background:   is an important cause of pediatric meningitis.

Objective:  The aim of this study was to analyze the clinical features and outcomes of children with pneumococcal meningitis at our hospital in China, so as to provide basis for improving the clinical treatment effect.

Methods:  This retrospective analysis included patients aged <16 years treated for pneumococcal meningitis at the Department of Neurology, Children's Hospital of Shanxi (January 2014-February 2016). Read More

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October 2021

Autoantibody of interleukin-17A induced by recombinant attenuates airway inflammation in mice with neutrophilic asthma.

J Asthma 2021 Oct 13:1-11. Epub 2021 Oct 13.

Department of Pediatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

: Previous studies have shown Interleukin (IL)-17A as an important contributor to the development of severe asthma, which is mainly characterized by neutrophilic inflammation and less response to corticosteroids. Consequently, the IL-17A-neutrophil axis could be a potential therapeutic target. Previously, we constructed a recombinant (rMS) expressing fusion protein Ag85A-IL-17A, and confirmed it could induce production of IL-17A autoantibody . Read More

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October 2021

Next-generation sequencing of cerebrospinal fluid for diagnosis of atypical herpes simplex encephalitis.

J Int Med Res 2021 Oct;49(10):3000605211049645

Department of Neurology, Harrison International Peace Hospital, Hengshui, Hebei, China.

Objectives: Herpes simplex encephalitis (HSE) is one of the most common causes of severe viral encephalitis. The characteristic manifestations of HSE include cerebrospinal fluid with mild cytopenia, dominated by lymphocytes, elevated protein, and normal blood glucose values (3.9-6. Read More

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October 2021

Chloride transport modulators as drug candidates.

Am J Physiol Cell Physiol 2021 Oct 13. Epub 2021 Oct 13.

Telethon Institute of Genetics and Medicine (TIGEM), Campi Flegrei 34, 80078, Pozzuoli, NA, Italy; Department of Translational Medical Sciences, University of Naples,, Naples, Italy.

Chloride transport across cell membranes is broadly involved in epithelial fluid transport, cell volume and pH regulation, muscle contraction, membrane excitability, and organellar acidification. The human genome encodes at least 53 chloride transporting proteins with expression in cell plasma or intracellular membranes, which include chloride channels, exchangers and cotransporters, some having broad anion specificity. Loss of function mutations in chloride transporters cause a wide variety of human diseases, including cystic fibrosis, secretory diarrhea, kidney stones, salt wasting nephropathy, myotonia, osteopetrosis, hearing loss and goiter. Read More

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October 2021

Fully 3D printed fluidic devices with integrated valves and pumps for flow injection analysis.

Anal Methods 2021 Oct 13. Epub 2021 Oct 13.

Department of Chemistry, Saint Louis University, USA.

The use of a PolyJet 3D printer to create a microfluidic device that has integrated valves and pumps is described. The process uses liquid support and stacked printing to result in fully printed devices that are ready to use within minutes of fabrication after minimal post-processing. A unique feature of PolyJet printing is the ability to incorporate several different materials of varying properties into one print. Read More

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October 2021

The Dynamic Interactions between Nanoparticles and Macrophages Impact Their Fate in Brain Tumors.

Small 2021 Oct 12:e2103600. Epub 2021 Oct 12.

Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Tongji University School of Medicine, Collaborative Innovation Center for Brain Science, Tongji University, 1800 Yuntai Road, Shanghai, 200123, China.

Functional nanomaterials such as iron oxide nanoparticles have been extensively explored for the diagnosis and treatment of central nervous system diseases. However, an insufficient understanding of the comprehensive nanomaterial-biological interactions in the brain hinders the nanomaterials from meeting the medical requirements for translational research. Here, FDA-approved ferumoxytol, an iron oxide nanoparticle, is chosen as the model nanomaterial for a systematic study of the dynamic interactions between ferumoxytol and immune cells, including microglia and macrophages, in the brain tumors. Read More

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October 2021

Fluidic bacterial diodes rectify magnetotactic cell motility in porous environments.

Nat Commun 2021 Oct 12;12(1):5949. Epub 2021 Oct 12.

Department of Mechanical Engineering, Tufts University, 200 College Avenue, Medford, MA, 02155, USA.

Directed motility enables swimming microbes to navigate their environment for resources via chemo-, photo-, and magneto-taxis. However, directed motility competes with fluid flow in porous microbial habitats, affecting biofilm formation and disease transmission. Despite this broad importance, a microscopic understanding of how directed motility impacts the transport of microswimmers in flows through constricted pores remains unknown. Read More

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October 2021

Genomic and transcriptomic correlates of immunotherapy response within the tumor microenvironment of leptomeningeal metastases.

Nat Commun 2021 Oct 12;12(1):5955. Epub 2021 Oct 12.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.

Leptomeningeal disease (LMD) is a devastating complication of solid tumor malignancies, with dire prognosis and no effective systemic treatment options. Over the past decade, the incidence of LMD has steadily increased due to therapeutics that have extended the survival of cancer patients, highlighting the need for new interventions. To examine the efficacy of immune checkpoint inhibitors (ICI) in patients with LMD, we completed two phase II clinical trials. Read More

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October 2021

Genomic comparison between cerebrospinal fluid and primary tumor revealed the genetic events associated with brain metastasis in lung adenocarcinoma.

Cell Death Dis 2021 Oct 12;12(10):935. Epub 2021 Oct 12.

Department of Neurosurgery, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Hunan Province, Changsha, 410013, China.

Lung adenocarcinoma (LUAD) is most common pathological type of lung cancer. LUAD with brain metastases (BMs) usually have poor prognosis. To identify the potential genetic factors associated with BM, a genomic comparison for BM cerebrospinal fluid (CSF) and primary lung tumor samples obtained from 1082 early- and late-stage LUAD patients was performed. Read More

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October 2021

Fluid flow exposure promotes epithelial-to-mesenchymal transition and adhesion of breast cancer cells to endothelial cells.

Breast Cancer Res 2021 Oct 12;23(1):97. Epub 2021 Oct 12.

Cellular and Molecular Bioengineering Research Lab, University of Calgary, Calgary, AB, Canada.

Background: Mechanical interactions between tumor cells and microenvironments are frequent phenomena during breast cancer progression, however, it is not well understood how these interactions affect Epithelial-to-Mesenchymal Transition (EMT). EMT is associated with the progression of most carcinomas through induction of new transcriptional programs within affected epithelial cells, resulting in cells becoming more motile and adhesive to endothelial cells.

Methods: MDA-MB-231, SK-BR-3, BT-474, and MCF-7 cells and normal Human Mammary Epithelial Cells (HMECs) were exposed to fluid flow in a parallel-plate bioreactor system. Read More

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October 2021

Personalized analysis of minimal residual cancer cells in peritoneal lavage fluid predicts peritoneal dissemination of gastric cancer.

J Hematol Oncol 2021 Oct 12;14(1):164. Epub 2021 Oct 12.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Panjiayuan nanli 17, Chaoyang District, Beijing, 100021, China.

Peritoneal dissemination (PD) is a major type of gastric cancer (GC) recurrence and leads to rapid death. Current approaches cannot precisely determine which patients are at high risk of PD to provide early intervention. In this study, we developed a technology to detect minimal residual cancer cells in peritoneal lavage fluid (PLF) samples with a personalized assay profiling tumor-specific mutations. Read More

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October 2021

Intraoperative radiation therapy induces immune response activity after pancreatic surgery.

BMC Cancer 2021 Oct 12;21(1):1097. Epub 2021 Oct 12.

Department of Surgery, Gangnam Severance Hospital, Yonsei University College of Medicine, 20, Eonju-ro 63 gil, Gangnam-gu, Seoul, 06229, South Korea.

Background: Pancreatic cancer has highly aggressive features, such as local recurrence that leads to significantly high morbidity and mortality and recurrence after successful tumour resection. Intraoperative radiation therapy (IORT), which delivers targeted radiation to a tumour bed, is known to reduce local recurrence by directly killing tumour cells and modifying the tumour microenvironment.

Methods: Among 30 patients diagnosed with pancreatic cancer, 17 patients received IORT immediately after surgical resection. Read More

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October 2021

Longitudinal Bottom-Up Proteomics of Serum, Serum Extracellular Vesicles, and Cerebrospinal Fluid Reveals Candidate Biomarkers for Early Detection of Glioblastoma in a Murine Model.

Molecules 2021 Oct 2;26(19). Epub 2021 Oct 2.

Fondazione Pisana per la Scienza ONLUS, 56017 San Giuliano Terme, Italy.

Glioblastoma Multiforme (GBM) is a brain tumor with a poor prognosis and low survival rates. GBM is diagnosed at an advanced stage, so little information is available on the early stage of the disease and few improvements have been made for earlier diagnosis. Longitudinal murine models are a promising platform for biomarker discovery as they allow access to the early stages of the disease. Read More

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October 2021

Chitinase Chit62J4 Essential for Chitin Processing by Human Microbiome Bacterium J4.

Molecules 2021 Oct 2;26(19). Epub 2021 Oct 2.

Laboratory of Anaerobic Microbiology, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, v. v. i., Vídeňská 1083, 142 00 Prague, Czech Republic.

Commensal bacterium J4 produces several extracellular chitinolytic enzymes including a 62 kDa chitinase Chit62J4 active toward 4-nitrophenyl ,'-diacetyl-β-d-chitobioside (pNGG). We characterized the crude enzyme from bacterial culture fluid, recombinant enzyme rChit62J4, and its catalytic domain rChit62J4cat. This major chitinase, securing nutrition of the bacterium in the human intestinal tract when supplied with chitin, has a pH optimum of 5. Read More

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October 2021