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No re-calibration required? Stability of a bioelectrochemical sensor for biodegradable organic matter over 800 days.

Biosens Bioelectron 2021 Jun 4;190:113392. Epub 2021 Jun 4.

School of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne, UK. Electronic address:

Microbial Fuel Cells (MFCs) operated as biosensors could potentially enable truly low-cost, real-time monitoring of organic loading in wastewaters. The current generated by MFCs has been correlated with conventional measures of organic load such as Biochemical Oxygen Demand (BOD), but much remains to be established in terms of the reliability and applicability of such sensors. In this study, batch-mode and multi-stage, flow-mode MFCs were operated for over 800 days and regularly re-calibrated with synthetic wastewater containing glucose and glutamic acid (GGA). Read More

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miR-424 induces apoptosis in glioblastoma cells and targets AKT1 and RAF1 oncogenes from the ERBB signaling pathway.

Eur J Pharmacol 2021 Jun 18:174273. Epub 2021 Jun 18.

- Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address:

Glioblastoma is a lethal and incurable cancer. Tumor suppressor miRNAs are promising gene therapy tools for cancer treatment. In silico, we predicted miR-424 as a tumor suppressor. Read More

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Diagnostic utility of flow cytometry based coated-platelets assay as a biomarker to predict thrombotic or hemorrhagic phenotype in acute stroke.

Cytometry B Clin Cytom 2021 Jun 21. Epub 2021 Jun 21.

Department of Transfusion Medicine and Immunohematology, Christian Medical College, Vellore, Tamil Nadu, India.

Background: Coated-platelets are sub-population of platelets "coated" with highly procoagulant proteins and phosphatidylserine that sustains thrombin generation. They are produced upon dual agonist stimulation by collagen and thrombin. This study was conducted to assess if there was any difference in the levels of coated-platelets in patients with primary intracranial hemorrhage (PICH) and ischemic stroke due to large artery atherosclerosis (LAA) as compared to healthy controls, and to see if coated-platelet levels had any influence on the hemorrhagic transformation (HT) of ischemic stroke. Read More

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Phagocytosis checkpoints on hematopoietic stem cells in patients with myelodysplastic syndromes.

Asia Pac J Clin Oncol 2021 Jun 21. Epub 2021 Jun 21.

Department of Hematology, General Hospital, Tianjin Medical University, Tianjin, China.

Background: The myelodysplastic syndrome (MDS) is a high-risk hemocytopenia easily converted to acute myeloid leukemia. CD47 plays an important role in regulating phagocytosis, and its role in the pathogenesis of MDS is unclear.

Methods: CD47 and PI3K/AKT/mTOR on CD34 CD38 cells were detected by flow cytometry. Read More

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O-GlcNAcylation in early stages of chronic lymphocytic leukemia; protocol development for flow cytometry.

Cancer Biomark 2021 Jun 19. Epub 2021 Jun 19.

Department of Laboratory Medicine, Medical School, University of Pécs, Pécs, Hungary.

Background: Recent studies proved that metabolic changes in malignant disorders have an impact on protein glycosylation, however, only a few attempts have been made so far to use O-GlcNAc analysis as a prognostic tool. Glucose metabolism is reported to be altered in hematological malignancies thus, we hypothesized that monitoring intracellular O-GlcNAc levels in Rai stage 0-I (Binet A) CLL patients could give deeper insights regarding subtle metabolic changes of progression which are not completely detected by the routine follow-up procedures.

Objective: In this proof of concept study we established a flow cytometric detection method for the assessment of O-GlcNAcylation as a possible prognostic marker in CLL malignancy which was supported by fluorescence microscopy. Read More

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The effect of brace treatment on pulmonary functions in adolescent idiopathic scoliosis: An 8-month follow-up study.

J Back Musculoskelet Rehabil 2021 Jun 18. Epub 2021 Jun 18.

Physiotherapy and Rehabilitation Department, Faculty of Health Sciences, Eastern Mediterranean University, Mersin, Turkey.

Background: The instant effect of a brace on pulmonary functions of patients with adolescent idiopathic scoliosis (AIS) is known. However, the permanent effects of its regular use are still unclear.

Objective: This study aimed to determine whether a brace in patients with AIS had a permanent effect on respiratory functions. Read More

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Effects of structured home-based exercise training on circulating endothelial progenitor cells and endothelial function in patients with intermittent claudication.

Vasc Med 2021 Jun 21:1358863X211020822. Epub 2021 Jun 21.

Unit of Internal Medicine, Angiology and Atherosclerosis, Department of Medicine and Surgery, University of Perugia, Perugia, Italy.

Introduction: Exercise training improves walking capacity in patients with intermittent claudication (IC). Endothelial progenitor cells (EPCs), endothelial microparticles (EMPs), and endothelial dysfunction could play a role in this process.

Methods: We measured EPCs and EMPs in a group of 60 patients with IC, and in a control group of 20 individuals without IC, before a treadmill test and 2, 24, and 48 hours after the test. Read More

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Serum HBV pregenomic RNA exhibited opposite associations with NKdim and NKbright cell immunity in treatment-naïve chronic hepatitis B patients.

Biosci Rep 2021 Jun 21. Epub 2021 Jun 21.

Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a new biomarker that reflects HBV replication, but its relationship with natural killer (NK) cell immunity in chronic hepatitis B (CHB) is unknown. We assessed serum HBV pgRNA levels in 323 CHB patients by reverse transcription-polymerase chain reaction, assessed cytokine production and activation and inhibitory markers of NK cells by flow cytometry, and measured serum cytokines by enzyme-linked immunosorbent assays. Among the different CHB phases, the serum HBV pgRNA level was highest in the immune tolerant (IT) and immune active (IA) phases. Read More

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Discovery of a microbial rhodopsin that is the most stable in extreme environments.

iScience 2021 Jun 24;24(6):102620. Epub 2021 May 24.

Department of Life Science and Institute of Biological Interfaces, Sogang University, Seoul 04107, Korea.

Microbial rhodopsin is a retinal protein that functions as an ion pump, channel, and sensory transducer, as well as a light sensor, as in biosensors and biochips. Tara76 rhodopsin is a typical proton-pumping rhodopsin that exhibits strong stability against extreme pH, detergent, temperature, salt stress, and dehydration stress and even under dual and triple conditions. Tara76 rhodopsin has a thermal stability approximately 20 times higher than that of thermal rhodopsin at 80°C and is even stable at 85°C. Read More

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Cancer-Preventive Role of Bone Marrow-Derived Mesenchymal Stem Cells on Colitis-Associated Colorectal Cancer: Roles of Gut Microbiota Involved.

Front Cell Dev Biol 2021 4;9:642948. Epub 2021 Jun 4.

Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Mesenchymal stem cells (MSCs) treatment showed promising results in inflammatory bowel disease in both rodent models and patients. Nevertheless, previous studies conducted conflicting results on preclinical tumor models treated with MSCs concerning their influence on tumor initiation and progression. This study is designed to demonstrate the role of bone marrow-derived MSCs and the potential mechanism in the colitis-associated colon cancer (CAC) model. Read More

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An Integrated Analysis of the Response of Colorectal Adenocarcinoma Caco-2 Cells to X-Ray Exposure.

Front Oncol 2021 3;11:688919. Epub 2021 Jun 3.

Laboratory of Radiation Biophysics and Radiobiology, Department of Physics, University of Pavia, Pavia, Italy.

Colorectal cancer is among the three top cancer types for incidence and the second in terms of mortality, usually managed with surgery, chemotherapy and radiotherapy. In particular, radiotherapeutic concepts are crucial for the management of advanced rectal cancer, but patients' survival remains poor, despite advances in treatment modalities. The use of well-characterized cell culture systems offers an important preclinical strategy to study mechanisms at the basis of cell response to therapeutic agents, including ionizing radiation, possibly leading to a better understanding of the response to the treatment. Read More

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3,3'-Diindolylmethane Enhances Paclitaxel Sensitivity by Suppressing DNMT1-Mediated KLF4 Methylation in Breast Cancer.

Front Oncol 2021 3;11:627856. Epub 2021 Jun 3.

Laboratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Paclitaxel (PTX) is a first-line chemotherapeutic drug for the treatment of breast cancer, but drug resistance seriously limits its clinical use. The aim of the present work was to explore the effect of 3,3'-diindolylmethane (DIM) on PTX sensitivity and its possible mechanism in breast cancer. The expression of Krüppel-like factor 4 (KLF4) and DNA-methyltransferase 1 (DNMT1) in breast cancer tissues were assessed by immunohistochemistry and Western blotting. Read More

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Autophagy dysregulation mediates the damage of high glucose to retinal pigment epithelium cells.

Int J Ophthalmol 2021 18;14(6):805-811. Epub 2021 Jun 18.

Department of Ophthalmology, the First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, Shaanxi Province, China.

Aim: To observe the role and mechanism of autophagy in retinal pigment epithelial cell (RPE) damaged by high glucose, so as to offer a new idea for the treatment of diabetic retinopathy (DR).

Methods: ARPE-19, a human RPE cell line cultured was divided into the normal control (NC), autophagy inhibitor 3-methyladenine (3-MA), high-glucose (HG), and HG+3-MA groups. Cell viability was detected by CCK-8 assay and the apoptosis rate was measured by flow cytometry. Read More

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Evaluation of iron and manganese removal effectiveness by treatment plant modules based on water pollution index; a comprehensive approach.

J Environ Health Sci Eng 2021 Jun 2;19(1):1005-1013. Epub 2021 May 2.

Civil, Water and Environmental Engineering Faculty, Shahid Beheshti University, 19839-69411, Tehran, Iran.

Groundwater is a viable alternative when access to surface water resources is limited. Iron and manganese are known ions in soil and naturally in groundwater sources. However, human activities also are responsible. Read More

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Removal of volatile organic compounds (VOCs) from waste air stream using ozone assisted zinc oxide (ZnO) nanoparticles coated on zeolite.

J Environ Health Sci Eng 2021 Jun 23;19(1):771-780. Epub 2021 Mar 23.

Faculty of Marine Science and Technology, North Tehran Branch, Islamic Azad University, Tehran, Iran.

The release of volatile organic compounds (VOCs) from stationary and mobile sources increases the concentration of these pollutants in the environment. These compounds have the potential to cause adverse effects on human health and the environment. The adoption of management and engineering procedures to control the emission of these pollutants to the air has become essential. Read More

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The effect of quercetin on cervical cancer cells as determined by inducing tumor endoplasmic reticulum stress and apoptosis and its mechanism of action.

Am J Transl Res 2021 15;13(5):5240-5247. Epub 2021 May 15.

Department of Gynecology, Gansu Provincial Maternity and Child-care Hospital Lanzhou 730050, Gansu Province, P. R. China.

Objective: This study aimed to explore the effect of quercetin on cervical cancer cells by inducing tumor endoplasmic reticulum stress (ERS) and apoptosis and its mechanism of action.

Methods: HeLa cells were treated with different concentrations of quercetin, and the cell viability was measured using methyl thiazolyl tetrazolium (MTT) colorimetric assays. The apoptosis rate was measured using flow cytometry. Read More

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The effect of targeted regulation of LATS2 by LncRNA BCAR4 on proliferation, migration and apoptosis of HCC cells.

Am J Transl Res 2021 15;13(5):4624-4631. Epub 2021 May 15.

Department of Hepatobiliary Surgery, Huizhou Municipal Central Hospital Huizhou 516001, Guangdong, China.

Objective: This study explored and analyzed the effects of targeted regulation of LATS2 by LncRNA BCAR4 on the proliferation, migration and apoptosis of hepatocellular carcinoma (HCC).

Method: We detected the expression of LncRNA, BCAR4 and LATS2 mRNA in liver hepatocellular carcinoma HepG2 cells and normal hepatocellular cells LO2 by RT-PCR. HepG2 cells were divided into BCAR4-siRNA, NC-siRNA and control groups. Read More

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Exosomes containing miR-122-5p secreted by LPS-induced neutrophils regulate the apoptosis and permeability of brain microvascular endothelial cells by targeting OCLN.

Am J Transl Res 2021 15;13(5):4167-4181. Epub 2021 May 15.

Department of Pediatric, Affiliated Hospital of Youjiang Medical University for Nationalities Baise, Guangxi Zhuang Autonomous Region, China.

Objective: To explore the effect of exosomes containing miR-122-5p secreted by lipopolysaccharide (LPS)-induced neutrophils on the apoptosis and permeability of brain microvascular endothelial cells (BMECs).

Methods: Neutrophils in blood were isolated, purified and identified. LPS-induced neutrophils were co-cultured with BMECs. Read More

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Hydroxysafflor Yellow A inhibits the viability and migration of vascular smooth muscle cells induced by serum from rats with chronic renal failure via inactivation of the PI3K/Akt signaling pathway.

Exp Ther Med 2021 Aug 8;22(2):850. Epub 2021 Jun 8.

Department of Nephrology, Lianyungang Hospital of Traditional Chinese Medicine, Lianyungang, Jiangsu 222000, P.R. China.

It has been reported that the viability and migration of vascular smooth muscle cells contributes to arteriovenous fistula stenosis. Hydroxysafflor Yellow A (HSYA) has been demonstrated to inhibit the viability and migration of VSMCs by regulating Akt signaling. The present study aimed to investigate the role of HSYA on the viability and migration of human umbilical vein smooth muscle cells (HUVSMCs) following stimulation using serum from rats with chronic renal failure (CRF), and to determine the effects of HSYA on PI3K/Akt signaling. Read More

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Inhibition of Exosome Release Sensitizes U937 Cells to PEGylated Liposomal Doxorubicin.

Front Immunol 2021 4;12:692654. Epub 2021 Jun 4.

Department of Pharmacology and Toxicology, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

Aims: Acute myeloblastic leukemia (AML) is the most common type of acute leukemia in adults. Despite numerous treatment strategies including chemotherapy and radiotherapy, a large number of patients do not respond to treatment and experience relapse. The main problem of these patients is the development of resistance to anti-cancer drugs. Read More

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The Frequency and Effect of Granulocytic Myeloid-Derived Suppressor Cells on Mycobacterial Survival in Patients With Tuberculosis: A Preliminary Report.

Front Immunol 2021 1;12:676679. Epub 2021 Jun 1.

Centre for Lung Infection and Immunity, Division of Pulmonology, Department of Medicine and UCT Lung Institute & South African MRC/UCT Centre for the Study of Antimicrobial Resistance, University of Cape Town, Cape Town, South Africa.

Introduction: Protective host responses in those exposed to or infected with tuberculosis (TB) is thought to require a delicate balance between pro-inflammatory and regulatory immune responses. Myeloid-derived suppressor cells (MDSCs), regulatory cells that dampen T-cell function, have been described in cancer and other infectious diseases but there are limited data on their role in TB.

Methods: Peripheral blood was obtained from patients with active pulmonary TB and participants with presumed latent TB infection (LTBI) from Cape Town, South Africa. Read More

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Measuring the Impact of Targeting FcRn-Mediated IgG Recycling on Donor-Specific Alloantibodies in a Sensitized NHP Model.

Front Immunol 2021 2;12:660900. Epub 2021 Jun 2.

Duke Transplant Center, Department of Surgery, Duke University Medical Center, Durham, NC, United States.

Background: In transplantation, plasmapheresis and IVIg provide the mainstay of treatment directed at reducing or removing circulating donor-specific antibody (DSA), yet both have limitations. We sought to test the efficacy of targeting the IgG recycling mechanism of the neonatal Fc receptor (FcRn) using anti-FcRn mAb therapy in a sensitized non-human primate (NHP) model, as a pharmacological means of lowering DSA.

Methods: Six (6) rhesus macaque monkeys, previously sensitized by skin transplantation, received a single dose of 30mg/kg anti-RhFcRn IV, and effects on total IgG, as well as DSA IgG, were measured, in addition to IgM and protective immunity. Read More

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Reversal of Abnormal CD4+ T Cell Metabolism Alleviates Thyroiditis by Deactivating the mTOR/HIF1a/Glycolysis Pathway.

Front Endocrinol (Lausanne) 2021 4;12:659738. Epub 2021 Jun 4.

Department of Endocrinology and Metabolism, The Endocrine Institute and The Liaoning Provincial Key Laboratory of Endocrine Diseases, NHC Key Laboratory of Diagnosis and Treatment of Thyroid Diseases, The First Hospital of China Medical University, Shenyang, China.

Background: Hashimoto's thyroiditis (HT) is an autoimmune disease that features activation of thyroid antigen-specific helper T cells. HT patients have increased Th1 and Th17 T cell subsets. Glycolysis supports chronic activation of Th1 and Th17 T cells, but how this contributes to HT remains unknown. Read More

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A Comparison of CVR Magnitude and Delay Assessed at 1.5 and 3T in Patients With Cerebral Small Vessel Disease.

Front Physiol 2021 2;12:644837. Epub 2021 Jun 2.

Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, United Kingdom.

Background: Cerebrovascular reactivity (CVR) measures blood flow change in response to a vasoactive stimulus. Impairment is associated with several neurological conditions and can be measured using blood oxygen level-dependent (BOLD) magnetic resonance imaging (MRI). Field strength affects the BOLD signal, but the effect on CVR is unquantified in patient populations. Read More

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LINC00665/miR-379-5p/GRP78 regulates cisplatin sensitivity in gastric cancer by modulating endoplasmic reticulum stress.

Cytotechnology 2021 Jun 26;73(3):413-422. Epub 2021 Apr 26.

Department of Thoracic Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210029 Jiangsu Province China.

Acquired resistance to cisplatin (DDP)-based chemotherapy greatly hinders the treatment of gastric cancer (GC). LINC00665 serves as an oncogene in GC. Hence, the current study was designed to investigate the regulatory effects of LINC00665 on DDP-resistance of GC. Read More

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[Imaging Parameter Optimization of 3D Radial Stack-of-stars MRA with FID Sampling after Treatment of Cerebral Aneurysms with Metallic Devices].

Nihon Hoshasen Gijutsu Gakkai Zasshi 2021 ;77(6):572-580

Department of Radiology, Eastern Chiba Medical Center.

Magnetic resonance angiography (MRA) using ultra-short TE (uTE) is known to be used for the evaluation of cerebral aneurysm after treatment such as clipping and coiling. However, conventional uTE sequences are not appropriate as an additional imaging sequence for 3D time-of-flight (TOF)-MRA because it is not possible to shorten scan time and acquire selective-volume imaging. To solve the problem, we focused on the combination of uTE sampling and 3D radial scan sequences. Read More

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January 2021

Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis.

J Vet Diagn Invest 2021 Jun 19:10406387211025513. Epub 2021 Jun 19.

Section of Clinical Pathology, Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.

Canine parvoviral enteritis (CPE) is a severe disease characterized by systemic inflammation and immunosuppression. The function of circulating phagocytes (neutrophils and monocytes) in affected dogs has not been fully investigated. We characterized the functional capacity of canine phagocytes in CPE by determining their oxidative burst and phagocytic activities using flow cytometry. Read More

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Understanding of plasticity size-effect governed mechanical response and incomplete die filling in a microscale double-punch molding configuration.

Int J Mech Sci 2020 Apr 23;172. Epub 2019 Dec 23.

Department of Mechanical & Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, U.S.A.

Direct replication of microscale patterns onto metal surfaces by compression molding with patterned dies is used to fabricate metal-based structures for microsystem applications. Micron scale plasticity governs both the mechanical molding response and the geometric fidelity of replicated patterns. Microscale molding replication offers a technologically relevant example in which various plasticity size effects manifest themselves and control the effectiveness of the fabrication process. Read More

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DRASTIC model adjusted with lineament density to map groundwater vulnerability: a case study in Rania basin, Kurdistan, Iraq.

Environ Sci Pollut Res Int 2021 Jun 19. Epub 2021 Jun 19.

Department of Geology, University of Sulaimani, Sulaymaniyah, Iraq.

Groundwater has never been heavily relied on as a water source in Northern Iraq as it has been in the last two decades due to the rapid and often unplanned urbanization, industrial and agricultural projects. This paper attempts to present a concise groundwater vulnerability assessment of Rania basin to the local and regional planning authorities to ensure a more sustainable development in the area. The focus of the study is the Rania basin, which is a part of Dokan subbasin in northeast Iraq. Read More

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Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children.

Ecotoxicol Environ Saf 2021 Jun 17;221:112430. Epub 2021 Jun 17.

Department of Environmental and Occupational Health, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM, Serdang, Selangor, Malaysia.

The exposure of school children to indoor air pollutants has increased allergy and respiratory diseases. The objective of this study were to determine the toxicodynamic interaction of indoor pollutants exposure, biological and chemical with expression of adhesion molecules on eosinophil and neutrophil. A self-administered questionnaire, allergy skin test, and fractional exhaled nitric oxide (FeNO) analyser were used to collect information on health status, sensitization to allergens and respiratory inflammation, respectively among school children at age of 14 years. Read More

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