858,090 results match your criteria flow cytometryresults

Development of a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay for the Detection of Haemonchus contortus in Goat Feces.

Korean J Parasitol 2021 Apr 22;59(2):167-171. Epub 2021 Apr 22.

Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.

Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. Read More

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Effect of Inhibition of the JAK2/STAT3 Signaling Pathway on the Th17/IL-17 Axis in Acute Cellular Rejection After Heart Transplantation in Mice.

J Cardiovasc Pharmacol 2021 Apr 7;77(5):614-620. Epub 2021 Apr 7.

Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.

Abstract: Acute immune rejection is one of the most serious complications of heart transplantation, and its mechanism has always been a hot spot. Th17 cells and cytokine interleukin-17 (IL-17) have been proved to be involved in acute immune rejection, and the signaling pathway mechanism has attracted our interest. It has been confirmed that the Janus kinase 2-signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway is involved in the differentiation of CD4+ T cells, so we focus on whether the JAK2/STAT3 signaling pathway is involved in the occurrence of acute immune rejection by regulating the Th17/IL-17 axis. Read More

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Circ_0000345 Protects Endothelial Cells From Oxidized Low-Density Lipoprotein-Induced Injury by miR-129-5p/Ten-Eleven Translocation Axis.

J Cardiovasc Pharmacol 2021 May;77(5):603-613

Dartpartment of Cardiovascular Surgery, The Third People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China.

Abstract: Circular RNAs have shown regulatory functions in atherosclerosis (AS) progression. Here, we explored the role and working mechanism of circ_0000345 in the AS cell model in vitro. Quantitative real-time polymerase chain reaction was applied to measure the enrichment of circ_0000345, microRNA-129-5p (miR-129-5p), and ten-eleven translocation-2 (TET2) messenger RNA. Read More

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Circular RNA circ_0010729 Knockdown Attenuates Oxygen-Glucose Deprivation-Induced Human Cardiac Myocytes Injury by miR-338-3p/CALM2 Axis.

J Cardiovasc Pharmacol 2021 May;77(5):594-602

Department of Cardiovascular III, Cangzhou Central Hospital, Cangzhou, Hebei, China.

Abstract: Circular RNAs have pivotal roles in cardiovascular disease. The injury of cardiac myocytes is associated with occurrence of cardiovascular disease. Circular RNA hsa_circ_0010729 (circ_0010729) is associated with cardiac myocytes injury. Read More

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Bulbar Urethral Stents for Bulbar Urethral Strictures: Long-Term Follow-Up after Stent Removal.

Urol Int 2021 May 5:1-6. Epub 2021 May 5.

Department of Urology, Kocaeli University School of Medicine, Kocaeli, Turkey.

Background/aim: The aim of this study was to assess long-term clinical efficacy of temporary bulbar urethral stent (BUS) used for treatment of recurrent bulbar urethral stricture (US).

Materials And Methods: A total of 168 patients with recurrent bulbar US who underwent BUS placement after internal urethrotomy between 2009 and 2019 were enrolled. An indwelling time of 12 months was planned for the stents. Read More

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Pseudomonas aeruginosa Pneumonia Causes a Loss of Type-3 and an Increase in Type-1 Innate Lymphoid Cells in the Gut.

J Surg Res 2021 May 2;265:212-222. Epub 2021 May 2.

Department of Surgery, Washington University School of Medicine, Saint Louis, MO. Electronic address:

Background: Sepsis induces gut barrier dysfunction characterized by increased gut epithelial apoptosis and increased intestinal permeability. The cytokine IL-22 has been demonstrated to regulate gut barrier function. Type-3 innate lymphoid cells (ILC3) are the predominate source of IL-22 in the GI tract. Read More

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Biofouling control: the impact of biofilm dispersal and membrane flushing.

Water Res 2021 Apr 18;198:117163. Epub 2021 Apr 18.

Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands; Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911 MA Leeuwarden, The Netherlands. Electronic address:

Pure culture studies have shown that biofilm dispersal can be triggered if the nutrient supply is discontinued by stopping the flow. Stimulating biofilm dispersal in this manner would provide a sustainable manner to control unwanted biofilm growth in industrial settings, for instance on synthetic membranes used to purify water. The response of multispecies biofilms to nutrient limitation has not been thoroughly studied. Read More

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Pristimerin synergistically sensitizes conditionally reprogrammed patient derived-primary hepatocellular carcinoma cells to sorafenib through endoplasmic reticulum stress and ROS generation by modulating Akt/FoxO1/p27 signaling pathway.

Phytomedicine 2021 Apr 4;86:153563. Epub 2021 Apr 4.

Department of Pharmacy, The First Affiliated Hospital, Sun Yat-sen University, 510080 Guangzhou, China. Electronic address:

Background: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-associated mortality worldwide. Sorafenib (SORA), as a first-line therapeutic drug, has been used to treat HCC, but resistance poses a major limitation on the efficacy of SORA chemotherapy. Pristimerin (PRIS), a natural bioactive component isolated from various plant species in the Celastraceae and Hippocrateaceae families, has been reported to exhibit outstanding antitumor effects in several types of cells in vitro. Read More

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Immunological and inflammatory profiles during acute and convalescent phases of severe/ critically ill COVID-19 patients.

Int Immunopharmacol 2021 Apr 17;97:107685. Epub 2021 Apr 17.

Department of Laboratory Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, China. Electronic address:

Background: The 2019 Coronavirus (COVID-19) pandemic poses a huge threat internationally; however, the role of the host immune system in the pathogenesis of COVID-19 is not well understood.

Methods: Cytokine and chemokine levels and characterisation of immune cell subsets from 20 COVID-19 cases after hospital admission (17 critically ill and 3 severe patients) and 16 convalescent patients were determined using a multiplex immunoassay and flow cytometry, respectively.

Results: IP-10, MCP-1, MIG, IL-6, and IL-10 levels were significantly higher in acute severe/critically ill patients with COVID-19, whereas were normal in patients who had reached convalescence. Read More

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PD-1 blockade combined with IL-33 enhances the antitumor immune response in a type-1 lymphocyte-mediated manner.

Cancer Treat Res Commun 2021 Apr 23;28:100379. Epub 2021 Apr 23.

Department of Immunology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China; Jiangsu Key Laboratory of Clinical Immunology, Soochow University, Suzhou 215006, China; Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address:

PD-1 immune checkpoint blockade and cytokine IL-33 have shown significant therapeutic effects in tumor immunotherapy. These therapies promote CD8 T cell activation, proliferation, and effector functions. However, there were few research about the combined therapy efficacy. Read More

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In vitro effects of bis(N-[4-(hydroxyphenyl)methyl]-2-pyridinemethamine)zinc perchlorate monohydrate 4 on the physiology and interaction process of Leishmania amazonensis.

Parasitol Int 2021 May 2:102376. Epub 2021 May 2.

Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Programa de Pós-Graduação em Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Leishmaniasis is one of the most relevant neglected tropical diseases in the world, affecting 14 million people. Despite the high morbidity, mortality and socio-economic impact, few therapeutic options are available for this disease. To make matters worse, the available molecules have several limitations such as limited efficacy, high cost, side effects and increased resistance. Read More

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Carotid endarterectomy with stent graft removal and end to end bypass in the treatment of in-stent occlusion after endovascular therapy of carotid gunshot injury.

Ann Vasc Surg 2021 May 2. Epub 2021 May 2.

Cardiac Surgery, Peking University Third Hospital, Beijing, China.

Carotid jugular fistula and pseudoaneurysm are easy to form after gunshot injury of carotid artery. Endovascular therapy, such as stent graft implantation, can successfully block the fistula and pseudoaneurysm cavity. However, the possibility of in-stent restenosis or occlusion will lead to treatment failure. Read More

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Odor identity can be extracted from the reciprocal connectivity between olfactory bulb and piriform cortex in humans.

Neuroimage 2021 May 2:118130. Epub 2021 May 2.

Department of Clinical Neuroscience, Karolinska Institutet, 17177, Stockholm, Sweden; Monell Chemical Senses Center, Philadelphia, PA, 19104, USA; Department of Psychology, University of Pennsylvania, Philadelphia, PA, 19104, USA; Stockholm University Brain Imaging Centre, Stockholm University, 10691, Stockholm, Sweden. Electronic address:

Neuronal oscillations route external and internal information across brain regions. In the olfactory system, the two central nodes-the olfactory bulb (OB) and the piriform cortex (PC)-communicate with each other via neural oscillations to shape the olfactory percept. Communication between these nodes have been well characterized in non-human animals but less is known about their role in the human olfactory system. Read More

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Comparison of Myeloablative Versus Reduced-Intensity Conditioning Regimens in Allogeneic Stem Cell Transplant Recipients with Acute Myelogenous Leukemia with MRD Negative Disease at the Time of Transplant: A Retrospective Cohort Study.

Transplant Cell Ther 2021 May 2. Epub 2021 May 2.

Blood and Marrow Transplant Center, AdventHealth Orlando Hospital, Orlando, FL 32804, USA. Electronic address:

Background/objective: The ideal conditioning intensity in allogeneic hematopoietic stem cell transplantation (HSCT) is evolving. Previous prospective studies comparing myeloablative versus reduced intensity conditioning regimens in adults with acute myelogenous leukemia (AML) have shown mixed results. In many of these studies, patients were not stratified based on measurable residual disease (MRD). Read More

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Can 3D pseudo-continuous arterial spin labeling perfusion imaging be applied to predict early response to chemoradiotherapy in patients with advanced nasopharyngeal carcinoma?

Radiother Oncol 2021 May 2. Epub 2021 May 2.

GE Healthcare, MR Research China, Beijing, 100176, China.

Background And Purpose: Chemoradiotherapy (CRT) has been widely applied in patients with advanced nasopharyngeal carcinoma (ANPC). However, limited imaging modality exists on the evaluation of early response to CRT. The purpose of this study was therefore to investigate whether 3D pseudo-continuous arterial spin labeling (3D pCASL) perfusion imaging could predict early response to CRT in ANPC patients. Read More

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IL-10 producing B cells regulated 1,3-β-glucan induced Th responses in coordinated with Treg.

Immunol Lett 2021 May 2. Epub 2021 May 2.

Division of Pneumoconiosis, School of Public Health, China Medical University, Shenyang, Liaoning, 110122, PR China. Electronic address:

Repeated exposure to fungi-contaminated dust can lead to multiple adverse effects on the lung, such as hypersensitivity pneumonitis, granuloma even irreversible fibrosis. 1,3-β-glucan, a major cell wall component of fungi, is considered as its exposure biomarker. Existing studies showed that a series of Th responses were involved in 1,3-β-glucan induced hypersensitivity pneumonitis, in which macrophages, Treg, and IL-10 producing B cells were reported to participate. Read More

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The effect of muscle blood flow restriction on hemodynamics, cerebral oxygenation and activation at rest.

Appl Physiol Nutr Metab 2021 May 5. Epub 2021 May 5.

National and Kapodistrian University of Athens, 68993, School of Physical Education and Sport Science, Athens, Attica, Greece;

This study tested the hypothesis that muscle blood flow restriction reduces muscle and cerebral oxygenation, at rest. In 26 healthy males, aged 33±2 yrs, physiological variables were continuously recorded during a 10-min period in two experimental conditions: a) with muscle blood flow restriction through thigh cuffs application inflated at 120 mmHg (With Cuffs, WC) and b) without restriction (No Cuffs, NC). Muscle and cerebral oxygenation were reduced by muscle blood flow restriction as suggested by the increase in both muscle and cerebral deoxygenated hemoglobin (Δ[HHb]; p<0. Read More

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microRNA-125b and its downstream Smurf1/KLF2/ATF2 axis as important promoters on neurological function recovery in rats with spinal cord injury.

J Cell Mol Med 2021 May 5. Epub 2021 May 5.

Department of Spine Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.

The purpose of this study is to investigate the role of microRNA-125b (miR-125b) and its mechanism in spinal cord injury (SCI) by targeting Smurf1. After loss- and gain-function approaches were conducted in SCI rat models and neural stem cells (NSCs) isolated from foetal rats, the Basso-Beattie-Bresnahan (BBB) score was calculated, and related protein expression was determined by Western blot analysis and cell apoptosis by TUNEL staining. NSC viability was detected by CCK-8, migration abilities by Transwell assay and apoptosis by flow cytometry. Read More

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Multicenter clinical evaluation of a piezoresistive-MEMS-sensor rapid-exchange pressure microcatheter system for fractional flow reserve measurement.

Catheter Cardiovasc Interv 2021 May 5. Epub 2021 May 5.

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, China.

Objectives: This multicenter, prospective clinical study investigates whether the microelectromechanical-systems-(MEMS)-sensor pressure microcatheter (MEMS-PMC) is comparable to a conventional pressure wire in fractional flow reserve (FFR) measurement.

Background: As a conventional tool for FFR measurement, pressure wires (PWs) still have some limitations such as suboptimal handling characteristics and unable to maintain the wire position during pullback assessment. Recently, a MEMS-PMC compatible with any 0. Read More

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Long-term impacts of drain spacing, crop management, and weather on nitrate leaching to subsurface drains.

J Environ Qual 2021 Mar 9. Epub 2021 Mar 9.

Dep. of Agronomy, Purdue Univ., 915 W. State St., West Lafayette, IN, 47907, USA.

Subsurface drainage is an essential water management practice for many poorly drained soils in the U.S. Midwest, but this practice also contributes nitrate-N loads to surface waters. Read More

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Does vascular endothelial dysfunction play a role in physical frailty and sarcopenia? A systematic review.

Age Ageing 2021 May;50(3):725-732

Department of Cardiology, Blacktown Hospital, Western Sydney Local Health District, Sydney, Australia.

Background: Frailty is strongly associated with adverse cardiovascular outcomes; however, the underlying pathophysiological processes are largely unknown. Vascular endothelial dysfunction (VED) is the earliest stage of cardiovascular disease (CVD) progression and predicts long-term CVD outcomes. Both these conditions share an elevated inflammatory state as a common pathological factor. Read More

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Laminar flow substantially affects the morphology and functional phenotype of glomerular endothelial cells.

PLoS One 2021 5;16(5):e0251129. Epub 2021 May 5.

Department of Nephrology, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Medical Center, Nijmegen, The Netherlands.

Shear stress induced by laminar blood flow has a profound effect on the morphology and functional phenotype of macrovascular endothelial cells. The influence of laminar flow on the glomerular microvascular endothelium, however, remains largely elusive. The glomerular endothelium, including its glycocalyx, is a crucial part of the glomerular filtration barrier, which is involved in blood filtration. Read More

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Potential value of urine lateral-flow lipoarabinomannan (LAM) test for diagnosing tuberculosis among severely acute malnourished children.

PLoS One 2021 5;16(5):e0250933. Epub 2021 May 5.

Epicentre, Paris, France.

Background: Tuberculosis (TB) is a serious co-morbidity among children with severe acute malnutrition (SAM) and TB diagnosis remains particularly challenging in the very young. We explored whether, in a low HIV-prevalence setting, the detection of mycobacterial lipoarabinomannan (LAM) antigen in urine may assist TB diagnosis in SAM children, a pediatric population currently not included in LAM-testing recommendations. To that end, we assessed LAM test-positivity among SAM children with and without signs or symptoms of TB. Read More

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Application and side effects of blood flow restriction technique: A cross-sectional questionnaire survey of professionals.

Medicine (Baltimore) 2021 May;100(18):e25794

Master of Science in Physical Education, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte.

Abstract: The physiological benefits of applying blood flow restriction (BFR) in isolation or in the presence of physical exercise have been widely documented in the scientific literature. Most investigations carried out under controlled laboratory conditions have found the technique to be safe. However, few studies have analyzed the use of the technique in clinical settings. Read More

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Long event-free survival after anti-BCMA CAR-T cell treatment for relapsed and refractory multiple myeloma patients: Two case reports.

Medicine (Baltimore) 2021 May;100(18):e25784

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R.China.

Introduction: Chimeric antigen receptor T (CAR-T) cells targeting B-cell maturation antigen (BCMA) have been used in the treatment of relapsed and refractory multiple myeloma (RRMM). The response rate and the depth of responses induced by anti-BCMA CAR-T cells are impressive. However, despite this, remissions are not sustained, and the majority of patients eventually relapse. Read More

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A breastfeeding woman with spontaneous coronary artery dissection and possible takotsubo syndrome: A case report.

Medicine (Baltimore) 2021 May;100(18):e25775

Department of Cardiology, Hiroshima Red Cross Hospital and Atomic-bomb Survivors Hospital, Hiroshima, Japan.

Rationale: The relationship between spontaneous coronary artery dissection (SCAD) and takotsubo syndrome (TTS) remains unclear. Coexistence of SCAD and TTS has been reported in the literature. However, the relationship between these two diseases has not yet been elucidated. Read More

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The role of E255K/V-inclusive mutations in a Philadelphia-positive acute lymphoblastic leukemia with mutation evolution during sequential TKIs therapies: A case report.

Medicine (Baltimore) 2021 May;100(18):e25579

Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan.

Rationale: Until recently, the survival rate in patients with Philadelphia-positive acute lymphoblastic leukemia (Ph+ ALL) was approximately 30%. Tyrosine kinase inhibitors (TKIs), which are a new class of drugs that target BCR-ABL fusion protein, have shown to be effective in treating Ph+ ALL in adults. However, the resistance mechanisms that promote the disease recurrence have altered the initial success of these revolutionary agents. Read More

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The diagnostic reliability and validity of noninvasive imaging modalities to assess leptomeningeal collateral flow for ischemic stroke patients: A systematic review and meta-analysis.

Medicine (Baltimore) 2021 May;100(18):e25543

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

Abstract: Leptomeningeal collateral flow (LMF) is associated with infarct area and clinical outcome for ischemic stroke patients. Although LMF can be detected by multiple imaging methods, but their diagnostic performance is uncertain.The aim of this study was to evaluate the diagnostic validity or reliability of noninvasive image methods in assessing LMF. Read More

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The research for the function evaluation of facial nerve and the mechanisms of rehabilitation training.

Medicine (Baltimore) 2021 May;100(18):e25430

Guangzhou University of Chinese Medicine, Guangzhou, China.

Background: Peripheral facial paralysis (PFP) is a common peripheral neural disease. Acupuncture treatment combined with PFP rehabilitation exercises is a routine method of PFP treatment. This article is to provide a new visual and objective evaluation method for exploring the mechanism and efficacy of acupuncture treatment on PFP, and develop an interactive augmented facial nerve function rehabilitation training system with multiple training models. Read More

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Thromboembolic Complications of Vascular Catheters Used for Pediatric Continuous Renal Replacement Therapy: Prevalence in a Single-Center, Retrospective Cohort.

Pediatr Crit Care Med 2021 May 4. Epub 2021 May 4.

Department of Perioperative Medicine and Intensive Care, Karolinska University Hospital, Huddinge, Stockholm, Sweden. Department of Physiology and Pharmacology, Section of Anaesthesia and Intensive Care, Karolinska Institutet, Stockholm, Sweden. Department of Pediatric Perioperative Medicine and Intensive Care, Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden. Department of Clinical Science Intervention and Technology (CLINTEC), Karolinska Institutet, Stockholm, Sweden.

Objectives: Pediatric venous thromboembolic events are commonly associated with in situ central venous catheters. The risk for severe venous thromboembolism increases if a larger portion of the vessel lumen is occupied by the central venous catheter. A functioning vascular catheter is required when the continuous renal replacement therapy is used in critically ill children. Read More

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