230,359 results match your criteria flow cytometry

Oral cytological changes in young adults related to alcohol consumption.

Arch Oral Biol 2021 Apr 16;126:105127. Epub 2021 Apr 16.

Programa de Pós graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil; Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil; Centro Regional de Estudos, Prevenção e Recuperação de Dependentes Químicos - CENPRE Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil. Electronic address:

Objective: The study aimed to investigate the oral cytological changes in young adults with recent history of alcohol consumption, as well as its relation with the consumption of alcohol.

Design: The sample included 67 young adults, who performed a smear of the oral mucosa and answered a questionnaire about recent and lifetime consumption of alcohol and other drugs. The methods used were sensitive to show the association between alcohol consumption and other drugs with the damage to oral cavity cells. Read More

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miR-760 mediated the proliferation and metastasis of hepatocellular carcinoma cells by regulating HMGA2.

Pathol Res Pract 2021 Mar 26;222:153420. Epub 2021 Mar 26.

Department of General Surgery, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, China; Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 226001, China. Electronic address:

Background: The purpose of our study was to investigate the roles of miR-760 and its potential mechanisms in HCC.

Methods: The functions of miR-760 were identified and measured by MTT, colony formation, transwell, and flow cytometry assays. Luciferase assay was applied to verify the direct binding of miR-760 on HMGA2 3'untranslated region (3'UTR). Read More

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LncRNA-cCSC1 promotes cell proliferation of colorectal cancer through sponging miR-124-3p and upregulating CD44.

Biochem Biophys Res Commun 2021 Apr 19;557:228-235. Epub 2021 Apr 19.

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. Electronic address:

LncRNA-cCSC1 is highly expressed in colorectal cancer (CRC). The study was designed to evaluate the function and mechanism of lncRNA-cCSC1 in cell proliferation of CRC. RT-PCR was used to measure the expression levels of lncRNA-cCSC1 in CRC cell lines. Read More

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Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Transl Res 2021 Apr 19. Epub 2021 Apr 19.

Oncogenetic laboratory; Sackler faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address:

Aberrant mesenchymal stem cells (MSCs) in multiple myeloma (MM) bone marrows (BM) promote disease progression and drug resistance. Here, we assayed the protein cargo transported from MM-MSCs to MM cells via microvesicles (MVs) with focus on ribosomal proteins (RPs) and assessment of their influence on translation initiation and design of MM phenotype. Proteomics analysis (mass spectrometry) demonstrated increased levels and repertoire of RPs in MM-MSCs MVs compared to normal donors (ND) counterparts (n=3-8; p= 9. Read More

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CyTOF: An Emerging Technology for Single-Cell Proteomics in the Mouse.

Curr Protoc 2021 Apr;1(4):e118

Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.

The ability to analyze the proteome of single cells is critical for the advancement of studies of steady-state and pathological processes. Mass cytometry, or CyTOF, combines principles of mass spectrometry and flow cytometry to enable single-cell analysis of protein expression. CyTOF can simultaneously assess DNA content and proteins and has the capacity to measure 40 to 100 parameters in each cell. Read More

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Impact of electric cardioversion on platelet activation.

PLoS One 2021 22;16(4):e0250353. Epub 2021 Apr 22.

Division of General Paediatrics, Department of Paediatrics and Adolescent Medicine, Medical University of Graz, Graz, Austria.

Introduction: Atrial fibrillation (AF) comes along with high risk of stroke. This risk continues even after re-establishing sinus rhythm with cardioversion. Aim of this study is to evaluate the contribution of electric cardioversion (EC) to platelet activation and procoagulatory tendency. Read More

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Assessment of salivary pro inflammatory cytokines profile level in patients treated with labial and lingual fixed orthodontic appliances.

PLoS One 2021 22;16(4):e0249999. Epub 2021 Apr 22.

Department of Orthodontics, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.

The secretions of certain cytokines, chemokines and growth factors are triggered by orthodontic appliances, which often affect the remodelling of periodontal tissues. Critical cumulative forces are applied by various types of orthodontic appliances to the periodontium. The secretion of such molecules is probably responsible, through molecular and cellular communications, for the optimal resorption of hard tissues in the periodontal setting, which therefore enables the coordination of multiple movements of tooth. Read More

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Octreotide inhibits secretion of IGF-1 from orbital fibroblasts in patients with thyroid-associated ophthalmopathy via inhibition of the NF-κB pathway.

PLoS One 2021 22;16(4):e0249988. Epub 2021 Apr 22.

Department of Ophthalmology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Purpose: We investigated the effect of octreotide, a long-acting somatostatin (SST) analogue, on IGF-1 secretion and its possible mechanism of action in orbital fibroblasts (OFs) from patients with thyroid-associated ophthalmopathy (TAO).

Materials And Methods: OFs were isolated from the orbital fat of patients with TAO or healthy individuals. The expression level of insulin-like growth factor (IGF)-1, at the protein and mRNA level, was determined with ELISA and quantitative RT-PCR, respectively. Read More

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Pitavastatin and metformin synergistically activate apoptosis and autophagy in pancreatic cancer cells.

Environ Toxicol 2021 Apr 22. Epub 2021 Apr 22.

Women's Health Research Laboratory, Changhua Christian Hospital, Changhua, Taiwan.

Pancreatic cancer is the seventh leading cause of cancer-related deaths globally. Metformin is the standard first-line of treatment for hyperglycemia in Type 2 diabetes, whereas pitavastatin is a cholesterol-lowering drug used to prevent cardiovascular diseases. Both these agents evidently exert anticancer effects on pancreatic cancer; however, it remains unclear whether cotreatment using them has additive or synergistic anticancer effects on pancreatic cancer. Read More

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LINC00943 knockdown exerts neuroprotective effects in Parkinson's disease through regulates CXCL12 expression by sponging miR-7-5p.

Genes Genomics 2021 Apr 22. Epub 2021 Apr 22.

Department of Neurology, Jingmen No. 1 People's Hospital, No. 168, Xiangshan Avenue, Duodao District, Jingmen, 448000, Hubei, China.

Background: Parkinson's disease (PD) is a common neurodegenerative movement disorder, but the pathogenesis is still unclear. Long non-coding RNAs (lncRNAs) have been reported to play a prominent role in PD.

Objective: This study is designed to explore the role and mechanism of long intergenic non-coding RNA 00943 (LINC00943) in the N-methyl-4-phenylpyridine (MPP)-inducted PD model. Read More

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Association of cell free mitochondrial DNA and caspase-1 expression with disease severity and ARTs efficacy in HIV infection.

Mol Biol Rep 2021 Apr 22. Epub 2021 Apr 22.

Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, University Road, Islamabad, 45320, Pakistan.

HIV infection is a global health concern. Current HIV-diagnostics provide information about the disease progression and efficacy of anti-retroviral therapies (ARVs), but this information is very limited and sometimes imprecise. Present study assessed the potential role of mononuclear cell (MNC) death, expression of caspases (1&3) and cell free mitochondrial DNA (CF mt-DNA) in HIV infected individuals. Read More

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Ouabain-induced activation of phospholipase C zeta and its contributions to bovine sperm capacitation.

Cell Tissue Res 2021 Apr 22. Epub 2021 Apr 22.

Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, CAL, T2N 4N1, Canada.

The sperm-derived oocyte activating factor, phospholipase C zeta (PLC ζ), is the only PLC isoform reported in cattle. The objectives were to (1) localize PLC ζ in fresh and capacitated bovine sperm and (2) investigate the activation of PLC ζ during bull sperm capacitation and contributions of PLC activity to this process. We confirmed interaction of testis-specific isoform of Na/K-ATPase (ATP1A4) with PLC ζ (immunolocalization and immunoprecipitation) and tyrosine phosphorylation (immunoprecipitation) of PLC ζ (a post-translational protein modification commonly involved in activation of PLC in somatic cells) during capacitation. Read More

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Long non-coding RNA NEAT1 promotes lipopolysaccharide-induced injury in human tubule epithelial cells by regulating miR-93-5p/TXNIP axis.

Med Microbiol Immunol 2021 Apr 22. Epub 2021 Apr 22.

Department of Respiratory and Critical Care Medicine, The Fourth People's Hospital of Shaanxi Province, No. 512, Xianning East Road, Xincheng District, Xi'an City, 710043, Shanxi Province, China.

Many long non-coding RNAs (lncRNAs) have been found to play crucial roles in sepsis-induced acute kidney injury (AKI), including lncRNA nuclear-enriched abundant transcript 1 (NEAT1). We aimed to further elucidate the functions and molecular mechanism of NEAT1 in sepsis-induced AKI. Sepsis-induced AKI cell model was established by treatment with lipopolysaccharide (LPS) in human tubule epithelial (HK2) cells. Read More

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Exhaustion in tumor-infiltrating Mucosal-Associated Invariant T (MAIT) cells from colon cancer patients.

Cancer Immunol Immunother 2021 Apr 22. Epub 2021 Apr 22.

Dept of Microbiology and Immunology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.

Mucosal-associated invariant T (MAIT) cells are unconventional T cells recognizing microbial metabolites, presented by the invariant MR1 protein. Upon activation, MAIT cells rapidly secrete cytokines and exert cytotoxic functions, and may thus be highly relevant also in tumor immunity. MAIT cells accumulate in colon tumors, but in contrast to other cytotoxic T cell subsets, their presence in tumors has been associated with worse patient outcome. Read More

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Effects of monochloramine on culturability, viability and persistence of Pseudomonas putida and tap water mixed bacterial community.

Appl Microbiol Biotechnol 2021 Apr 22. Epub 2021 Apr 22.

Department of Civil and Environmental Engineering, National University of Singapore, 1 Engineering Drive 2, #07-03 E1A, Singapore, 117576, Singapore.

Bacterial biofilms are able to persist in drinking water distribution systems (DWDS) even if disinfectants such as monochloramine are used to inhibit bacterial colonization and biofilm formation. While studies have determined the monochloramine concentrations required to inhibit bacterial biofilms, not much is known about how bacterial biofilms develop resistance towards monochloramine. This study covers the development of resistance to monochloramine in both single species and mixed bacterial biofilms. Read More

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Glucosamine attenuates drug resistance in Mitoxantrone-resistance breast cancer cells.

J Pharm Pharmacol 2021 Apr 22. Epub 2021 Apr 22.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad,Iran.

Objectives: This study was aimed at investigating the cytotoxicity and multi-drug resistance (MDR) reversal effect of Glucosamine (GlcN) on resistant BCRP-overexpressing breast cancer MCF-7/MX cells.

Methods: After confirming the overexpression of BCRP, the cytotoxicity and MDR reversing potential of GlcN on MCF-7/MX mitoxantrone-resistant and MCF-7 sensitive breast cancer cells were assessed via MTT assay. The effects of GlcN on mitoxantrone accumulation were analyzed through flow cytometry. Read More

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Atractylodin inhibited the migration and induced autophagy in cholangiocarcinoma cells via PI3K/AKT/mTOR and p38MAPK signalling pathways.

J Pharm Pharmacol 2021 Apr 22. Epub 2021 Apr 22.

Chulabhorn International College of Medicine, Thammasat University, Rangsit Center, Klong Luang, Pathum Thani, Thailand.

Objectives: The effects of atractylodin (ATD), the bioactive compound from Atractylodes lancea, on migration and autophagy status of cholangiocarcinoma cell line were investigated.

Methods: Cytotoxic activity and effects on cell migration and invasion were evaluated by MTT and trans-well assay, respectively. Autophagy and underlying molecular mechanisms were investigated using flow cytometry and western blot analysis. Read More

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Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response.

Environ Toxicol 2021 Apr 22. Epub 2021 Apr 22.

Department of General Biology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, Brazil.

Gastric cancer is the fifth most common malignancy worldwide. Serjania marginata Casar. (SM) displays anti-inflammatory properties and has been used to treat gastrointestinal disorders. Read More

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LncRNA DANCR targets miR-185-5p to upregulate LIM and SH3 protein 1 (LASP1) promoting prostate cancer via the FAK/PI3K/AKT/GSK3β/Snail pathway.

J Gene Med 2021 Apr 22:e3344. Epub 2021 Apr 22.

Department of Urology, The Second Hospital of Shandong University, Jinan City, Shandong, China.

Background: LncRNA DANCR acts as an oncogene in different cancers, but its roles in prostate cancer are not fully reported. We aim to explore its mechanism in facilitating the malignancy of prostate cancer.

Methods: The expressions of DANCR, miR-185-5p and LASP1 in 40 pairs of prostate cancer tissues and normal tissues, 5 prostate cancer cell lines and 1 epithelial cell line were assessed by qRT-PCR, western blot and immunohistochemistry respectively. Read More

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Tumor staging in a Beagle dog with concomitant large B-cell lymphoma and T-cell acute lymphoblastic leukemia.

J Vet Diagn Invest 2021 Apr 22:10406387211011024. Epub 2021 Apr 22.

Department of Veterinary Medicine, University of Milan, Lodi, Italy.

An 8-y-old spayed female Beagle dog was presented with peripheral lymphadenomegaly. Lymph node cytology and flow cytometry led to the diagnosis of large B-cell lymphoma (LBCL). We detected minimal percentages of LBCL cells in peripheral blood and bone marrow samples. Read More

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CD4+ and CD25+ T cell response to short-time interferon-beta therapy on IL10, IL23A and FOXP3 genes in multiple sclerosis patients.

Int J Clin Pract 2021 Apr 22:e14238. Epub 2021 Apr 22.

Department of Medical Biology, Faculty of Medicine, Yeditepe University, 34755, Istanbul, Turkey.

Aim Of The Study: Interferon-beta (IFN-β), multiple sclerosis (MS) drug for years, does not have therapeutic effects on each patient. Yet, a considerable portion has experienced no therapeutic response to IFN-β. Therefore, it is necessary to determine disease-specific biomarkers that affect drug response. Read More

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Profound dysregulation of T cell homeostasisand function in patients with severe COVID-19.

Allergy 2021 Apr 21. Epub 2021 Apr 21.

Department of Immunology, University Hospital Zurich (USZ), Zurich, Switzerland.

Background: Coronavirus disease 2019 (COVID-19) is caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and shows a broad clinical presentation ranging from asymptomatic infection to fatal disease. A very prominentfeature associated with severe COVID-19 is T cell lymphopenia. However, homeostatic and functional properties of T cells are ill-defined in COVID-19. Read More

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Macrophage depletion in stellate ganglia alleviates cardiac sympathetic overactivation and ventricular arrhythmogenesis by attenuating neuroinflammation in heart failure.

Basic Res Cardiol 2021 Apr 21;116(1):28. Epub 2021 Apr 21.

Department of Emergency Medicine, University of Nebraska Medical Center, Omaha, NE, 68198, USA.

Cardiac sympathetic overactivation is involved in arrhythmogenesis in patients with chronic heart failure (CHF). Inflammatory infiltration in the stellate ganglion (SG) is a critical factor for cardiac sympathoexcitation in patients with ventricular arrhythmias. This study aims to investigate if macrophage depletion in SGs decreases cardiac sympathetic overactivation and ventricular arrhythmogenesis in CHF. Read More

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Black pepper and piperine induce anticancer effects on leukemia cell line.

Toxicol Res (Camb) 2021 Mar 18;10(2):169-182. Epub 2021 Feb 18.

Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.

The black pepper, most commonly used in Indian cuisines for ages, is considered as "king of spices." The present study evaluates the anticancer potential of black pepper and its main constituent, i.e. Read More

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Polyphenols from Induce Apoptosis of HepG2 Cells via Inactivation of ERK and AKT Signaling Pathways.

Evid Based Complement Alternat Med 2021 23;2021:8841706. Epub 2021 Mar 23.

Department of Bioengineering, College of Food Science, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China.

The extract of has been proved to have antitumor activity. However, the underlying mechanism remains unclear. This study aimed to elucidate the mechanism of apoptosis of HepG2 cells induced by polyphenols from (PBPs). Read More

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MicroRNAs Expression Induces Apoptosis of Macrophages in Response to (MRHO/IR/75/ER): An In-Vitro and In-Vivo Study.

Iran J Parasitol 2020 Oct-Dec;15(4):475-487

Zoonosis Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Background: We aimed to investigate the effect of miR-15a mimic and inhibitor of miR-155 expression on apoptosis induction in macrophages infected with Iranian strain of in-vitro and in-vivo.

Methods: RAW 264.7 cells were infected with promastigotes (MRHO/IR/75/ER), and then were treated with miRNAs. Read More

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Angiotensin receptor blocker Losartan inhibits tumor growth of colorectal cancer.

EXCLI J 2021 1;20:506-521. Epub 2021 Mar 1.

Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

The renin-angiotensin system (RAS) is up-regulated in patients with colorectal cancer (CRC) and is reported to be associated with poor prognosis and chemo-resistance. Here we explored the therapeutic potential of targeting RAS in CRC using Losartan, an angiotensin receptor blocker. An integrative-systems biology approach was used to explore a proteome-level dataset of a gene signature that is modulated by Losartan. Read More

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Long Non-Coding RNA XIST Promotes Wilms Tumor Progression Through the miR-194-5p/YAP Axis.

Cancer Manag Res 2021 12;13:3171-3180. Epub 2021 Apr 12.

Department of Urology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, People's Republic of China.

Purpose: Although the long non-coding RNA (lncRNA) X inactive-specific transcript (XIST) has been reported to have an anti-tumor effect in multiple malignant tumors, its role in Wilms tumor (WT) progression has not been characterized. Thus, we investigated the underlying mechanism by which XIST regulates WT progression.

Patients And Methods: We performed microarray analysis and real-time quantitative PCR (RT-qPCR) to detect the expression levels of XIST lncRNA, microRNA-194-5p (miR-194-5p), and YAP (yes-associated protein in Hippo pathway) in tumor and matched adjacent normal tissues and blood collected from 49 WT patients. Read More

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Calycosin Induces Gastric Cancer Cell Apoptosis via the ROS-Mediated MAPK/STAT3/NF-κB Pathway.

Onco Targets Ther 2021 12;14:2505-2517. Epub 2021 Apr 12.

Department of Biochemistry and Molecular Biology, College of Life Science & Technology, Heilongjiang Bayi Agricultural University, Daqing, People's Republic of China.

Background: Calycosin, an active compound in plants, can promote the apoptosis of various cancer cells; however, the mechanism by which it regulates reactive oxygen species (ROS) in gastric cancer (GC) cells remains unclear.

Purpose: In this study, we investigated the effects of calycosin on apoptosis, the cell cycle, and migration in GC cells under ROS regulation.

Results: The results of the Cell Counting Kit-8 assay suggested that calycosin had significant cytotoxic effects on 12 gastric cancer cells, but no significant cytotoxic effects on normal cells. Read More

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Actin-Like Protein 8 Promotes the Progression of Triple-Negative Breast Cancer via Activating PI3K/AKT/mTOR Pathway.

Onco Targets Ther 2021 12;14:2463-2473. Epub 2021 Apr 12.

Breast and Thyroid Surgery, Dongying People's Hospital, Dongying, Shandong, 257091, People's Republic of China.

Objective: The purpose of this study was to investigate the function of actin-like protein 8 (ACTL8) on triple-negative breast cancer (TNBC) and its potential mechanisms.

Methods: In our study, ACTL8 expression and the prognostic values of ACTL8 were evaluated via the dataset from the Cancer Genome Atlas (TCGA). At the same time, the expression of ACTL8 in TNBC cells was measured by Western blot and qRT-PCR. Read More

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