J Mol Diagn 2021 Sep 22. Epub 2021 Sep 22.
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri. Electronic address:
The detection of recurrent gene fusions can help confirm diagnoses in solid tumors, particularly when the morphology and staining are unusual or nonspecific, and can guide therapeutic decisions. While fluorescence in situ hybridization (FISH) and PCR are often used to identify fusions, the rearrangement must be suspected, with only a few prioritized probes run. We hypothesized that the Illumina TruSight RNA Fusion Panel, which detects fusions of 507 genes and their partners, would uncover fusions with greater sensitivity than other approaches, leading to changes in diagnosis, prognosis, or therapy. Read More