31 results match your criteria fijiensis causal


The Mitochondrial Genome of a Plant Fungal Pathogen (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies.

Life (Basel) 2021 Mar 9;11(3). Epub 2021 Mar 9.

Corporación para Investigaciones Biológicas, Comparative Biology Laboratory, Cra 72A Medellín, Antioquia, Colombia.

Mycosphaerellaceae is a highly diverse fungal family containing a variety of pathogens affecting many economically important crops. Mitochondria play a crucial role in fungal metabolism and in the study of fungal evolution. This study aims to: (i) describe the mitochondrial genome of , and (ii) compare it with closely related species (, , and ) available online, paying particular attention to the Sigatoka disease's complex causal agents. Read More

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A world-wide analysis of reduced sensitivity to DMI fungicides in the banana pathogen Pseudocercospora fijiensis.

Pest Manag Sci 2021 Mar 25. Epub 2021 Mar 25.

Wageningen Research, Wageningen University and Research, Wageningen, The Netherlands.

Background: Pseudocercospora fijiensis is the causal agent of the black leaf streak disease (BLSD) of banana. Bananas are important global export commodities and a major staple food. Their susceptibility to BLSD pushes disease management towards excessive fungicide use, largely relying on multisite inhibitors and sterol demethylation inhibitors (DMIs). Read More

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Distribution of species causing Sigatoka leaf diseases of banana in Uganda and Tanzania.

Plant Pathol 2020 Jan 3;69(1):50-59. Epub 2019 Nov 3.

International Institute of Tropical Agriculture PO Box 7878 Kampala Uganda.

Sigatoka leaf diseases are a major constraint to banana production. A survey was conducted in Tanzania and Uganda to assess the distribution of species and severity of Sigatoka leaf diseases. species were identified using species-specific primers. Read More

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January 2020

Pfcyp51 exclusively determines reduced sensitivity to 14α-demethylase inhibitor fungicides in the banana black Sigatoka pathogen Pseudocercospora fijiensis.

PLoS One 2019 17;14(10):e0223858. Epub 2019 Oct 17.

Laboratory of Phytopathology, Wageningen University and Research, The Netherlands, Wageningen, the Netherlands.

The haploid fungus Pseudocercospora fijiensis causes black Sigatoka in banana and is chiefly controlled by extensive fungicide applications, threatening occupational health and the environment. The 14α-Demethylase Inhibitors (DMIs) are important disease control fungicides, but they lose sensitivity in a rather gradual fashion, suggesting an underlying polygenic genetic mechanism. In spite of this, evidence found thus far suggests that P. Read More

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A polyketide synthase gene cluster associated with the sexual reproductive cycle of the banana pathogen, Pseudocercospora fijiensis.

PLoS One 2019 25;14(7):e0220319. Epub 2019 Jul 25.

Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States of America.

Disease spread of Pseudocercospora fijiensis, causal agent of the black Sigatoka disease of banana, depends on ascospores produced through the sexual reproductive cycle. We used phylogenetic analysis to identify P. fijiensis homologs (PKS8-4 and Hybrid8-3) to the PKS4 polyketide synthases (PKS) from Neurospora crassa and Sordaria macrospora involved in sexual reproduction. Read More

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A novel polyketide synthase gene cluster in the plant pathogenic fungus Pseudocercospora fijiensis.

PLoS One 2019 8;14(2):e0212229. Epub 2019 Feb 8.

Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States of America.

Pseudocercospora fijiensis, causal agent of black Sigatoka of banana, produces polyketide synthase (PKS) pathways shown to be important in disease development by related Dothideomycete fungi. Genome analysis of the P. fijiensis PKS8-1 gene identified it as part of a gene cluster including genes encoding two transcription factors, a regulatory protein, a glyoxylase/beta-lactamase-like protein, an MFS transporter, a cytochrome P450, two aldo/keto reductases, a dehydrogenase, and a decarboxylase. Read More

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November 2019

Silencing of the Mitogen-Activated Protein Kinases (MAPK) and in Reduces Growth and Virulence on Host Plants.

Front Plant Sci 2018 13;9:291. Epub 2018 Mar 13.

International Institute of Tropical Agriculture, Nairobi, Kenya.

, causal agent of the black Sigatoka disease (BSD) of spp., has spread globally since its discovery in Fiji 1963 all the banana and plantain growing areas across the globe. It is becoming the most damaging and economically important disease of this crop. Read More

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A new mechanism for reduced sensitivity to demethylation-inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis.

Mol Plant Pathol 2018 06 13;19(6):1491-1503. Epub 2018 Feb 13.

Wageningen University and Research, Wageningen Plant Research, 6700 AA Wageningen, the Netherlands.

The Dothideomycete Pseudocercospora fijiensis, previously Mycosphaerella fijiensis, is the causal agent of black Sigatoka, one of the most destructive diseases of bananas and plantains. Disease management depends on fungicide applications, with a major contribution from sterol demethylation-inhibitors (DMIs). The continued use of DMIs places considerable selection pressure on natural P. Read More

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Population Structure of Pseudocercospora fijiensis in Costa Rica Reveals Shared Haplotype Diversity with Southeast Asian Populations.

Phytopathology 2017 12 9;107(12):1541-1548. Epub 2017 Oct 9.

First, second, third, and sixth authors: Department of Plant Pathology, North Carolina State University, Raleigh 27695; third author: Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29631; fourth author: Dole Standard Fruits, SA, Tropical Research Department, Rio Frio, Costa Rica; and fifth author: Agronomic Research Center, University of Costa Rica, San Pedro, Montes de Oca, Costa Rica.

Pseudocercospora fijiensis is the causal pathogen of black Sigatoka, a devastating disease of banana that can cause 20 to 80% yield loss in the absence of fungicides in banana crops. The genetic structure of populations of P. fijiensis in Costa Rica was examined and compared with Honduran and global populations to better understand migration patterns and inform management strategies. Read More

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December 2017

A comparative genome analysis of with other members of the pathogen genus on different plant hosts.

Genom Data 2017 Sep 8;13:54-63. Epub 2017 Jul 8.

Department of Plant Biology, University of Illinois, Urbana, IL 61801, USA.

Fungi are the causal agents of many of the world's most serious plant diseases causing disastrous consequences for large-scale agricultural production. Pathogenicity genomic basis is complex in fungi as multicellular eukaryotic pathogens. Here, we report the genome sequence of , and comparative genome analysis with plant pathogen members of the genus ( (synonyms ), , and - pathogens of wheat, pine, poplar and banana, respectively). Read More

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September 2017

Bioinformatics Prediction of Polyketide Synthase Gene Clusters from Mycosphaerella fijiensis.

PLoS One 2016 7;11(7):e0158471. Epub 2016 Jul 7.

Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, 27695-7612, United States of America.

Mycosphaerella fijiensis, causal agent of black Sigatoka disease of banana, is a Dothideomycete fungus closely related to fungi that produce polyketides important for plant pathogenicity. We utilized the M. fijiensis genome sequence to predict PKS genes and their gene clusters and make bioinformatics predictions about the types of compounds produced by these clusters. Read More

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Antifungal performance of extracellular chitinases and culture supernatants of Streptomyces galilaeus CFFSUR-B12 against Mycosphaerella fijiensis Morelet.

World J Microbiol Biotechnol 2016 Mar 12;32(3):44. Epub 2016 Feb 12.

El Colegio de la Frontera Sur (ECOSUR), Carr. Antiguo Aeropuerto km. 2.5, Apartado Postal 36, 30700, Tapachula, Chiapas, Mexico.

The tropical and mycoparasite strain Streptomyces galilaeus CFFSUR-B12 was evaluated as an antagonist of Mycosphaerella fijiensis Morelet, causal agent of the Black Sigatoka Disease (BSD) of banana. On zymograms of CFFSUR-B12 culture supernatants, we detected four chitinases of approximately 32 kDa (Chi32), 20 kDa (Chi20), and two with masses well over 170 kDa (ChiU) that showed little migration during denaturing electrophoresis at different concentrations of polyacrylamide. The thymol-sulphuric acid assay showed that the ChiU were glycosylated chitinases. Read More

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4-Phenylphenalenones as a template for new photodynamic compounds against Mycosphaerella fijiensis.

Pest Manag Sci 2016 Apr 6;72(4):796-800. Epub 2015 Jul 6.

Instituto de Química-Universidad de Antioquia, Medellín, Colombia.

Background: Evaluation of 4-phenylphenalenones and structural analogues against the fungal pathogen Mycosphaerella fijiensis (causal agent of black sigatoka disease in bananas) under light-controlled conditions uncovered some key structural features for the design of photodynamic compounds.

Results: Structure-activity relationship analysis revealed the importance of a chromophoric aryl-ketone and a steroidomimetic structural motif in the activity of the assayed compounds. The results pointed to 1,2-dihydro-3H-naphtho[2',1':3,4]cyclohepta[1,2-b]furan-3-one, which displayed an activity in the range of propiconazole but with photodynamic behaviour. Read More

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Positive selection and intragenic recombination contribute to high allelic diversity in effector genes of Mycosphaerella fijiensis, causal agent of the black leaf streak disease of banana.

Mol Plant Pathol 2014 Jun 16;15(5):447-60. Epub 2013 Dec 16.

Department of Plant Pathology, University of California Davis, One Shields Avenue, Davis, CA, 95616-8751, USA; Laboratory of Phytopathology, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, 6708PB, the Netherlands; Centre for BioSystems Genomics, PO Box 98, Wageningen, 6700 AB, the Netherlands.

Previously, we have determined the nonhost-mediated recognition of the MfAvr4 and MfEcp2 effector proteins from the banana pathogen Mycosphaerella fijiensis in tomato, by the cognate Cf-4 and Cf-Ecp2 resistance proteins, respectively. These two resistance proteins could thus mediate resistance against M. fijiensis if genetically transformed into banana (Musa spp. Read More

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Genome-wide in silico identification of GPI proteins in Mycosphaerella fijiensis and transcriptional analysis of two GPI-anchored β-1,3-glucanosyltransferases.

Mycologia 2013 Mar-Apr;105(2):285-96. Epub 2012 Sep 6.

Unidad de biotecnología, Centro de Investigación Científica de Yucatán, Calle 43, No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, México.

The hemibiotrophic fungus Mycosphaerella fijiensis is the causal agent of black Sigatoka (BS), the most devastating foliar disease in banana (Musa spp.) worldwide. Little is known about genes that are important during M. Read More

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First Report of Black Sigatoka Disease in Banana Caused by Mycosphaerella fijiensis on Martinique Island.

Plant Dis 2011 Mar;95(3):359

Service de la Protection des Végétaux, Marigot de Bellevue, 97205 Fort de France, Martinique, France.

Mycosphaerella fijiensis Morelet (anamorph Pseudocercospora fijiensis Morelet), the causal agent of black Sigatoka disease of banana, is considered to be the greatest economical threat for export-banana cultivation throughout the world because most cultivars are highly susceptible. The disease has a worldwide distribution throughout the humid tropical regions, but was still absent in some Caribbean islands hitherto. In Martinique Island, an intensive survey has been conducted by the plant protection service and the Fédération Régionale de Défense Contre les Organismes Nuisibles (FREDON) since April 2008 to detect as early as possible any outbreak of infection by M. Read More

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Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis-banana interaction.

Plant Cell Rep 2011 May 30;30(5):913-28. Epub 2011 Jan 30.

Instituto de Biotecnología de las Plantas, Universidad Central Marta Abreu de Las Villas, Carretera a Camajuaní km 5.5, 54 830, Santa Clara, Cuba.

Mycosphaerella fijiensis, a hemibiotrophic fungus, is the causal agent of black leaf streak disease, the most serious foliar disease of bananas and plantains. To analyze the compatible interaction of M. fijiensis with Musa spp. Read More

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Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp).

Genet Mol Res 2010 Nov 9;9(4):2207-12. Epub 2010 Nov 9.

Plant Research International B.V., Wageningen University and Research Centre, Wageningen, The Netherlands.

We searched the genome of Mycosphaerella fijiensis for molecular markers that would allow population genetics analysis of this plant pathogen. M. fijiensis, the causal agent of banana leaf streak disease, also known as black Sigatoka, is the most devastating pathogen attacking bananas (Musa spp). Read More

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November 2010

Tomato Cf resistance proteins mediate recognition of cognate homologous effectors from fungi pathogenic on dicots and monocots.

Proc Natl Acad Sci U S A 2010 Apr 5;107(16):7610-5. Epub 2010 Apr 5.

Laboratory of Phytopathology, Wageningen University and Research Centre, 6708 PB, Wageningen, The Netherlands.

Most fungal effectors characterized so far are species-specific and facilitate virulence on a particular host plant. During infection of its host tomato, Cladosporium fulvum secretes effectors that function as virulence factors in the absence of cognate Cf resistance proteins and induce effector-triggered immunity in their presence. Here we show that homologs of the C. Read More

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Oxidative stress response of Mycosphaerella fijiensis, the causal agent of black leaf streak disease in banana plants, to hydrogen peroxide and paraquat.

Can J Microbiol 2009 Jul;55(7):887-94

Departamento de Química ICET, Universidad Autónoma de Guadalajara, Patria 1201, Lomas del Valle 44100, Guadalajara, Jalisco, México.

Mycosphaerella fijiensis causes black leaf streak disease in banana and plantain. This fungus is usually attacked by reactive oxygen species secreted by the plant or during exposure to fungicide, however, little is known about the antioxidant response of the fungus. In this study, mycelia were observed to totally decompose 30 mmol/L of hydrogen peroxide (H2O2) within 120 min, liberating oxygen bubbles, and also to survive in concentrations as high as 100 mmol/L H2O2. Read More

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A Quantitative Assay Using Mycelial Fragments to Assess Virulence of Mycosphaerella fijiensis.

Phytopathology 2007 Aug;97(8):916-29

ABSTRACT We describe a method to evaluate the virulence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease (BLSD) of banana and plantain. The method is based on the delivery of weighed slurries of fragmented mycelia by camel's hair brush to 5-by-5-cm areas on the abaxial surface of banana leaf blades. Reliable BLSD development was attained in an environmental growth chamber with stringent lighting and humidity controls. Read More

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Construction of a genetic linkage map of the fungal pathogen of banana Mycosphaerella fijiensis, causal agent of black leaf streak disease.

Curr Genet 2008 May 26;53(5):299-311. Epub 2008 Mar 26.

Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130. Colonia Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.

A genetic linkage map of the fungal plant pathogen Mycosphaerella fijiensis, causal agent of black leaf streak disease of banana was developed. A cross between the isolates CIRAD86 (from Cameroon) and CIRAD139A (from Colombia) was analyzed using molecular markers and the MAT locus. The genetic linkage map consists of 298 AFLP and 16 SSR markers with 23 linkage groups, containing five or more markers, covering 1,879 cM. Read More

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Construction and characterization of a bacterial artificial chromosome library of the causal agent of Black Sigatoka fungal leaf spot disease of banana and plantain, Mycosphaerella fijiensis.

Mol Biotechnol 2007 May;36(1):64-70

Centro de Investigación Científica de Yucatán, Unidad de Biotecnología, Calle 43 No. 130, Col. Chuburná de Hidalgo, Mérida, Yucatán, 97200, México.

A bacterial artificial chromosome library of the causal agent of the Black Sigatoka leaf spot disease of banana and plantain, Mycosphaerella fijiensis, has been constructed using a non-sphaeroplasting technique and characterized using both homologous and heterologous probes. After first and a second size selection of PFGE-fractionated DNA, a ligation was obtained using a 1:4 molar ratio (insert:vector). One hundred random clones were analyzed, and the mean insert size was estimated to be 90 kb. Read More

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Rapid Screening of Musa Species for Resistance to Black Leaf Streak Using In Vitro Plantlets in Tubes and Detached Leaves.

Plant Dis 2007 Mar;91(3):308-314

IITA, PMB 5320, Ibadan, Nigeria.

This study investigated the utility of inoculation of in vitro plantlets in tubes and detached leaves as reliable and rapid assays for screening Musa genotypes against Mycosphaerella fijiensis, the causal agent of black leaf streak. In the first part of the study, three types of inocula were evaluated to determine suitability for in vitro inoculation. Inoculation of in vitro plantlets with mycelial fragments resulted in significantly (P < 0. Read More

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Isolation and characterization of the mating type locus of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana.

Mol Plant Pathol 2007 Jan;8(1):111-20

Centro de Investigación Científica de Yucatán (CICY), Calle 43 no. 130, Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, México.

SUMMARY Idiomorphs mat1-1 and mat1-2 from Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana, were isolated. Degenerate oligos were used to amplify the HMG box of the mat1-2 idiomorph from M. fijiensis, showing homology with the HMG box of Mycosphaerella graminicola. Read More

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January 2007

Mycosphaerella fijiensis, Causal Agent of Black Sigatoka of Musa spp. Found in Puerto Rico and Identified by Polymerase Chain Reaction.

Plant Dis 2006 May;90(5):684

University of Florida, IFAS, Tropical Research and Education Center, Homestead.

Black Sigatoka, also known as black leaf streak, is caused by Mycosphaerella fijiensis Morelet (anamorph Pseudocercospora fijiensis (Morelet) Deighton). It is the most significant disease of bananas and plantains (Musa spp.) because most of the economically important cultivars of exported and staple commodities are highly susceptible. Read More

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Development of a transformation system for Mycosphaerella pathogens of banana: a tool for the study of host/pathogen interactions.

FEMS Microbiol Lett 2001 Feb;195(1):9-15

Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853-1801, USA.

A genetic transformation system has been developed for three Mycosphaerella pathogens of banana and plantain (Musa spp.). Mycosphaerella fijiensis and Mycosphaerella musicola, the causal agents of black and yellow Sigatoka, respectively, and Mycosphaerella eumusae, which causes Septoria leaf spot of banana, were transformed with a construct carrying a synthetic gene encoding green fluorescent protein (GFP). Read More

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February 2001

Detection of Chlorothalonil in Dew Water Following Aerial Spray Application and Its Role in the Control of Black Sigatoka in Banana.

Plant Dis 1998 Nov;82(11):1191-1198

ISK Biosciences Corporation, La Lima, Honduras.

A standardized bioassay measuring the growth inhibition of Aspergillus niger in vitro allowed the detection of small concentrations (0.1 to 20 μg/ml) of chlorothalonil present in dew water on both the adaxial and abaxial surface of banana leaves in a commercial plantation receiving aerial sprays. Chlorothalonil concentrations detected in dew water on the banana leaf surface were within the range of concentrations required to prevent Mycosphaerella fijiensis (causal agent of black Sigatoka) ascospore germination in laboratory bioassays. Read More

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November 1998

Infection Studies of Mycosphaerella fijiensis on Banana and the Control of Black Sigatoka with Chlorothalonil.

Plant Dis 1998 Nov;82(11):1185-1190

ISK Biosciences Corporation, La Lima, Honduras.

Infection studies with Mycosphaerella fijiensis, causal agent of black Sigatoka disease of banana (Musa AAA), demonstrated that the abaxial leaf surface is the primary infection site. Inoculation of banana plants with M. fijiensis ascospores on the abaxial surface of young leaves resulted in disease symptoms in 100% of the leaves inoculated within 18 to 30 days; whereas only 5% of the leaves inoculated on the adaxial surface showed black Sigatoka symptoms within 10 weeks. Read More

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November 1998

Selection ofMycosphaerella fijiensis-resistant cell lines from micro-cross sections of banana and plantain.

Plant Cell Rep 1997 Feb;16(5):339-343

FG Phytomedizin, Humboldt University, Lentzeallee 55-57, D-14195, Berlin, Germany.

Anin vitro selection system using microcross sections of banana and plantain cultivars belonging to AAA and AAB genomic groups were used to produce plants resistant against the Black Sigatoka disease. The fungus resistant plantlets were obtained in a double selection system. This involved in a first step the use of a fungal crude filtrate and in the second step the purified host-specific toxin 2,4,8-trihydroxytetralone extracted from the fungusMycosphaerella fijiensis (M. Read More

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February 1997