337,891 results match your criteria fibroblast


Chemically Crosslinked Methylcellulose Substrates for Cell Sheet Engineering.

Gels 2021 Sep 14;7(3). Epub 2021 Sep 14.

Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Via Luigi Mancinelli 7, 20131 Milan, Italy.

Methylcellulose (MC) hydrogels have been successfully proposed in the field of cell sheet engineering (CSE), allowing cell detachment from their surface by lowering the temperature below their transition temperature (T). Among the main limitations of pristine MC hydrogels, low physical stability and mechanical performances limit the breadth of their potential applications. In this study, a crosslinking strategy based on citric acid (CA) was used to prepare thermoresponsive MC hydrogels, with different degrees of crosslinking, to exploit their possible use as substrates in CSE. Read More

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September 2021

A new cytotoxic sesquiterpene lactone from Euphorbia microsphaera Boiss against human breast cancer (MCF-7) and human fibrosarcoma (HT1080) cells.

Toxicon 2021 Sep 22. Epub 2021 Sep 22.

Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address:

A new guaianolide sesquiterpene lactone with cytotoxic properties was isolated from Euphorbia microsphaera Boiss. To determine the highest active fraction and isolate bioactive compounds, a bioassay guided fractionation approach was used. The general toxicity properties of the plant's extracts and fractions (fr) were assessed against Artemia salina, Oryzeaphilus mercator, and Tribolium castaneum. Read More

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September 2021

A Method for Orthotopic Transplantation of Lung Cancer in Mice.

Methods Mol Biol 2022 ;2374:231-242

Nancy B. and Jake L. Hamon Center for Therapeutic Oncology Research and Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Preclinical mouse models of lung cancer have been vital experimental tools to elucidate cancer biology and test novel therapeutic regimens. Two main models are most commonly used-genetically engineered mouse models and xenograft transplantation models. The most common xenograft model employs subcutaneous transplantation of tumor cells. Read More

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January 2022

The stimulatory effects of glucagon on cortisol and GH secretion occur independently from FGF-21.

Endocrine 2021 Sep 25. Epub 2021 Sep 25.

Yeditepe University Medical School Department of Endocrinology, Istanbul, Turkey.

Purpose: Glucagon stimulation test (GST) is used to assess the hypothalamo-pituitary-adrenal (HPA) and growth hormone (GH) axes with an incompletely defined mechanism. We aimed to assess if glucagon acted through fibroblast growth factor-21 (FGF-21) to stimulate cortisol and GH secretion. The secondary outcome was to determine the relationship of FGF-21 with variable GH responses to GST in obesity. Read More

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September 2021

TGFBI production by macrophages contributes to an immunosuppressive microenvironment in ovarian cancer.

Cancer Res 2021 Sep 24. Epub 2021 Sep 24.

Barts Cancer Institute, Queen Mary University of London

The tumor microenvironment evolves during malignant progression with major changes in non-malignant cells, cytokine networks, and the extracellular matrix (ECM). In this study, we aimed to understand how the ECM changes during neoplastic transformation of serous tubal intraepithelial carcinoma lesions (STIC) into high-grade serous ovarian cancers (HGSOC). Analysis of the mechanical properties of human fallopian tubes (FT) and ovaries revealed that normal FT and fimbria had a lower tissue modulus, a measure of stiffness, than normal or diseased ovaries. Read More

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September 2021

Chitosan - Rosmarinic acid conjugates with antioxidant, anti-inflammatory and photoprotective properties.

Carbohydr Polym 2021 Dec 1;273:118619. Epub 2021 Sep 1.

Group of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, Madrid, Spain; Networking Biomedical Research Centre in Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, Madrid, Spain. Electronic address:

Rosmarinic acid is an attractive candidate for skin applications because of its antioxidant, anti-inflammatory, and photoprotective functions, however, its poor bioavailability hampers its therapeutic outcome. In this context, synthesis of polymer conjugates is an alternative to enlarge its applications. This work describes the synthesis of novel water-soluble chitosan - rosmarinic acid conjugates (CSRA) that have great potential for skin applications. Read More

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December 2021

Mechanoadaptive injectable hydrogel based on poly(γ-glutamic acid) and hyaluronic acid regulates fibroblast migration for wound healing.

Carbohydr Polym 2021 Dec 26;273:118607. Epub 2021 Aug 26.

State Key Laboratory of Materials-Oriented Chemical Engineering, National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing 211816, China. Electronic address:

Injectable hydrogels have shown therapeutic effects on wound repair, but most of them exhibit poor mechanical strength. The impacts of stiff injectable hydrogels on cell behavior and wound healing remain unclear. Herein, an injectable hydrogel was developed based on thiolated poly(γ-glutamic acid) (γ-PGA-SH) and glycidyl methacrylate-conjuated oxidized hyaluronic acid (OHA-GMA). Read More

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December 2021

Cytotoxicity, anti-angiogenic, anti-tumor and molecular docking studies on phytochemicals isolated from Polygonum hydropiper L.

BMC Complement Med Ther 2021 Sep 24;21(1):239. Epub 2021 Sep 24.

Department of Pharmacy, Faculty of Biological Sciences, University of Malakand, Chakdara, 18000 Dir (L), KP, Pakistan.

Background: According to the recent global cancer statistics, breast cancer is the leading cause of deaths among women with 2.3 million new cases globally. Likewise, cervical cancer is also among the leading causes of mortality among women. Read More

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September 2021

Nesfatin-1 facilitates IL-1β production in osteoarthritis synovial fibroblasts by suppressing miR-204-5p synthesis through the AP-1 and NF-κB pathways.

Aging (Albany NY) 2021 Sep 24;13(undefined). Epub 2021 Sep 24.

Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.

The progression of osteoarthritis (OA) is mediated by adipokines, one of which is nesfatin-1, which is responsible for the production of inflammatory cytokines. However, how this molecule may affect the synthesis of the proinflammatory cytokine interleukin 1 beta (IL-1β) in OA is unclear. Our analyses of records from the Gene Expression Omnibus (GEO) dataset and clinical specimens of synovial tissue revealed higher levels of nesfatin-1 and IL-1β in OA samples compared with normal healthy tissue. Read More

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September 2021

Generation and characterization of iPSC lines from two nuclear envelopathy patients with a homozygous nonsense mutation in the TOR1AIP1 gene.

Stem Cell Res 2021 Sep 20;56:102539. Epub 2021 Sep 20.

The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel. Electronic address:

LAP1 is an inner nuclear membrane protein encoded by TOR1AIP1. A homozygous c.961C > T loss of function mutation in TOR1AIP1 that affects both isoforms of LAP1 was recently described. Read More

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September 2021

Toxicity effects of size fractions of incinerated sewage sludge bottom ash on human cell lines.

Environ Int 2021 Sep 21;158:106881. Epub 2021 Sep 21.

Energy and Environmental Sustainability Solutions for Megacities (E2S2), Campus for Research Excellence and Technological Enterprise (CREATE), 138602, Singapore; Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585, Singapore. Electronic address:

Sewage sludge bottom ash (SSBA) from the incineration plant used for the production of construction materials possibly possess heavy metals which might cause a negative impact on human health. Considering biosafety, we investigated the toxicity effects of 0.5-2 mm (aggregate substitute) and < 0. Read More

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September 2021

TGF-β-induced fibrosis: A review on the underlying mechanism and potential therapeutic strategies.

Eur J Pharmacol 2021 Sep 21;911:174510. Epub 2021 Sep 21.

Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43300, Malaysia. Electronic address:

Transforming growth factor-beta (TGF-β) plays multiple homeostatic roles in the regulation of inflammation, proliferation, differentiation and would healing of various tissues. Many studies have demonstrated that TGF-β stimulates activation and proliferation of fibroblasts, which result in extracellular matrix deposition. Its increased expression can result in many fibrotic diseases, and the level of expression is often correlated with disease severity. Read More

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September 2021

Isolation of neural stem and oligodendrocyte progenitor cells from the brain of live rats.

Stem Cell Reports 2021 Sep 8. Epub 2021 Sep 8.

Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, CB2 0AW Cambridge, UK; Lab of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, Greece. Electronic address:

Postnatal brain neural stem and progenitor cells (NSPCs) cluster in anatomically inaccessible stem cell niches, such as the subependymal zone (SEZ). Here, we describe a method for the isolation of NSPCs from live animals, which we term "milking." The intracerebroventricular injection of a release cocktail, containing neuraminidase, integrin-β1-blocking antibody, and fibroblast growth factor 2, induces the controlled flow of NSPCs in the cerebrospinal fluid, where they are collected via liquid biopsies. Read More

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September 2021

General anesthesia with cisatracurium and sevoflurane for a patient with primary carnitine deficiency receiving L-carnitine therapy: A case report.

Authors:
Ling Ai Yan Fang

Medicine (Baltimore) 2021 Sep;100(38):e27333

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

Rationale: Lipid storage myopathies (LSMs) are a series of genetic disorders of lipid metabolism predominantly affecting muscle. The low incidence and lethal properties of this disease make anesthesia experience limited in such patients. Among all etiologies of LSMs, primary carnitine deficiency (PCD) is now considered highly treatable by early administration of L-carnitine, though it remains unclear whether L-carnitine is effective enough to protect diseased muscle against conventionally used neuromuscular blocking agents (NMBAs) during general anesthesia. Read More

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September 2021

Radiotherapy for a rare phosphaturic mesenchymal tumor in the middle ear presenting with oncogenic osteomalacia: A case report.

Medicine (Baltimore) 2021 Sep;100(38):e27284

Department of Radiation Oncology, Narita Memorial Proton Center, Toyohashi, Japan.

Rationale: Phosphaturic mesenchymal tumor (PMT) is a rare neoplasm causing oncogenic osteomalacia. Surgery remains the definitive treatment for PMT, and radiotherapy is seldom employed. However, surgery for PMT involving the head and neck is often difficult due to the local invasion and complicated anatomy. Read More

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September 2021

Adipocytokines and Associations with Abnormal Body Composition in Rheumatoid Arthritis.

Arthritis Care Res (Hoboken) 2021 Sep 24. Epub 2021 Sep 24.

Columbia University, New York, NY, USA.

Purpose: We determined associations between adipokines and abnormal body composition in patients with rheumatoid arthritis (RA).

Methods: Combining data from three RA cohorts, whole-body dual-energy absorptiometry measures of appendicular lean mass and fat mass indices were converted to age, sex, and race-specific Z-Scores. Lean mass relative to fat mass was determined based on prior methods. Read More

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September 2021

Tinzaparin inhibits VL30 retrotransposition induced by oxidative stress and/or VEGF in HC11 mouse progenitor mammary cells: Association between inhibition of cancer stem cell proliferation and mammosphere disaggregation.

Oncol Rep 2021 Nov 24;46(5). Epub 2021 Sep 24.

Laboratory of General Biology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece.

Tinzaparin is an anticoagulant and antiangiogenic drug with inhibitory properties against tumor growth. VEGF stimulates angiogenesis, while an association between reactive oxygen species (ROS) and angiogenesis is involved in tumor progression. The present study aimed to investigate the effect of tinzaparin on VL30 retrotransposition‑positive mouse HC11 mammary stem‑like epithelial cells, previously reported to be associated with induced mammosphere/cancer stem cell (CSC) generation and tumorigenesis. Read More

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November 2021

Molecular characterisation and function analysis of gene from Yangzhou goose ().

Br Poult Sci 2021 Sep 24. Epub 2021 Sep 24.

Joint International Research Laboratory of Agriculture & Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, Jiangsu, China.

1. NOD1 is a significant member of the NOD-like receptor (NLR) family. Its main role is to identify microorganisms that invade the body, transmit immune signals and regulate innate immune responses. Read More

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September 2021

[Acupuncture intervention prolongates thrombolysis time window by regulating cerebral cortex angiogenesis-related genes and proteins in cerebral infarction rats].

Zhen Ci Yan Jiu 2021 Sep;46(9):751-6

College of Acupuncture and Massage, Nanjing University of Chinese Medicine, Nanjing 210023, China.

Objective: To observe the effect of acupuncture on the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and endostatin (Endostatin, ES) mRNAs and proteins (angiogenesis related factors) in the ischemic penumbra region in rats with cerebral infarction (CI), so as to explore its underlying mechanisms in prolonging the time window of thrombolysis therapy for CI.

Methods: A total of 48 male SD rats were randomly divided into sham operation, model, medication (6 h thrombolysis) and acupuncture (Acupunct)+medication groups (n=12 in each group). The CI model was established using modified auto-thrombus method. Read More

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September 2021

Silencing of Nesprin-2 inhibits the differentiation of myofibroblasts from fibroblasts induced by mechanical stretch.

Int Wound J 2021 Sep 23. Epub 2021 Sep 23.

Department of Burn and Plastic Surgery, the Affiliated Hospital of Qingdao University, Qingdao, China.

Mechanical force plays a pivotal role in the pathogenesis of hypertrophic scar (HTS). Dermal fibroblasts and myofibroblasts are the key cells involved in HTS. Myofibroblasts in HTS possess different biochemical and biophysical characteristics by which myofibroblasts are often distinguished from fibroblasts. Read More

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September 2021

Differential Modulation of the Voltage-Gated Na Channel 1.6 by Peptides Derived From Fibroblast Growth Factor 14.

Front Mol Biosci 2021 7;8:742903. Epub 2021 Sep 7.

Department of Pharmacology and Toxicology, Galveston, TX, United States.

The voltage-gated Na (Nav) channel is a primary molecular determinant of the initiation and propagation of the action potential. Despite the central role of the pore-forming α subunit in conferring this functionality, protein:protein interactions (PPI) between the α subunit and auxiliary proteins are necessary for the full physiological activity of Nav channels. In the central nervous system (CNS), one such PPI occurs between the C-terminal domain of the Nav1. Read More

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September 2021

Fibroblast growth factor 21 (FGF21) alleviates senescence, apoptosis, and extracellular matrix degradation in osteoarthritis via the SIRT1-mTOR signaling pathway.

Cell Death Dis 2021 Sep 23;12(10):865. Epub 2021 Sep 23.

Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, Zhejiang Province, China.

Osteoarthritis (OA) is a complex condition that involves both apoptosis and senescence and currently cannot be cured. Fibroblast growth factor 21 (FGF21), known for its role as a potent regulator of glucose and energy metabolism, protects from various diseases, possibly by mediating autophagy. In the present study, the role of FGF21 in the progression of OA was investigated in both in vitro and in vivo experiments. Read More

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September 2021

Synthesis, preclinical evaluation, and a pilot clinical PET imaging study of Ga-labeled FAPI dimer.

J Nucl Med 2021 Sep 23. Epub 2021 Sep 23.

The First Affiliated Hospital of Xiamen University, China.

Cancer-associated fibroblasts (CAFs) are crucial components of the tumor microenvironment. Fibroblast activation protein (FAP) is overexpressed in CAFs. FAP-targeted molecular imaging agents, including FAP inhibitor (FAPI)-04 and FAPI-46, have shown promising results in tumor diagnosis. Read More

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September 2021

Soluble Klotho and Incident Hypertension.

Clin J Am Soc Nephrol 2021 Sep 23. Epub 2021 Sep 23.

Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas

Background And Objectives: Hypertension is associated with significant morbidity and mortality despite effective antihypertensive therapies. Soluble klotho is a circulating protein that in preclinical studies is protective against the development of hypertension. There are limited studies of klotho and blood pressure in humans. Read More

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September 2021

Rolipram plays an anti-fibrotic effect in ligamentum flavum fibroblasts by inhibiting the activation of ERK1/2.

BMC Musculoskelet Disord 2021 Sep 23;22(1):818. Epub 2021 Sep 23.

Department of Orthopedics, Changzheng Hospital, Naval Medical University, Shanghai, 200003, People's Republic of China.

Background: Fibrosis is an important factor and process of ligamentum flavum hypertrophy. The expression of phosphodiesterase family (PDE) is related to inflammation and fibrosis. This article studied the expression of PDE in hypertrophic ligamentum flavum fibroblasts and investigated whether inhibition of PDE4 activity can play an anti-fibrotic effect. Read More

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September 2021

A standardized g-force allows the preparation of similar PRF qualities regardless of rotor angle.

Tissue Eng Part A 2021 Sep 24. Epub 2021 Sep 24.

Goethe-Universitat Frankfurt am Main, 9173, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University, Frankfurt Am Main, Germany, Frankfurt am Main, Hessen, Germany;

Platelet rich fibrin (PRF) is an autologous blood concentrate, that supports tissue regeneration. The effect of the centrifuge rotor angle in the fabrication of PRF is still not fully elucidated. The hypothesis of this study is: When applying the same g-force {relative centrifugal force (RCF)} and centrifugation time, PRF components and bioactivity are not modified using either a swing-out or fixed angle rotor. Read More

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September 2021

Endothelial Stromal PD-L1 (Programmed Death Ligand 1) Modulates CD8 T-Cell Infiltration After Heart Transplantation.

Circ Heart Fail 2021 Sep 24:CIRCHEARTFAILURE120007982. Epub 2021 Sep 24.

Department of Pathology, School of Medicine (W.B.-B., T.W., M.V.T., R.A.A., Q.Z., M.K.H., D.Č.).

Background: The role of checkpoint axes in transplantation has been partially addressed in animal models but not in humans. Occurrence of fulminant myocarditis with allorejection-like immunologic features in patients under anti-PD1 (programmed death cell protein 1) treatment suggests a key role of the PD1/PD-L1 (programmed death ligand 1) axis in cardiac immune homeostasis.

Methods: We cross-sectionally studied 23 heart transplant patients undergoing surveillance endomyocardial biopsy. Read More

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September 2021

IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury.

Int Immunopharmacol 2021 Sep 20;100:108142. Epub 2021 Sep 20.

Department of Anesthesiology, The First People's Hospital of Foshan, Foshan 528000, China. Electronic address:

The chronic phase following toxin-induced acute kidney injury (AKI) is characterized by robust inflammation and progressive kidney fibrosis. Interferon regulatory factor 4 (IRF-4) is a type of multifunctional transcription factor that has been deeply linked to inflammation and fibrotic diseases. However, the role of IRF-4 in kidney damage and renal fibrosis after toxin-induced AKI remain to be explored. Read More

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September 2021

Fabrication and characterization of a semi-rigid shell barrier system made of polycaprolactone and biphasic calcium phosphate: A novel barrier system for bone regeneration.

J Mech Behav Biomed Mater 2021 Sep 20;124:104841. Epub 2021 Sep 20.

Institute of Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Thailand.

Introduction: Nowadays, no barrier membrane serves all purposes of bone augmentation. This study aimed to fabricate a semi-rigid shell barrier system composed of a semi-rigid shell and a covering membrane or a semi-resorbable barrier membrane, based on polycaprolactone (PCL) and biphasic calcium phosphate (BCP) for guided bone regeneration (GBR).

Materials And Methods: A shell and a covering membrane were fabricated by a solvent casting technique based on PCL (70) and BCP (30). Read More

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September 2021

LIF and bFGF enhanced chicken primordial follicle activation by Wnt/β-catenin pathway.

Theriogenology 2021 Sep 16;176:1-11. Epub 2021 Sep 16.

Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address:

The cytokines leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF) are closely related to the development of primordial follicles. In this study, the functions and correlation of LIF and bFGF in the development of chicken primordial follicles were examined, along with the signaling pathways including protein kinase B (AKT), extracellular regulated protein kinase (ERK) and Wnt/β-catenin signaling pathways. Ovarian tissues were collected from four-day-old chicks and incubated with LIF and bFGF alone or in combination for three days to observe the changes in follicular development. Read More

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September 2021