2,069 results match your criteria favourable vitro


Development of Dexamethasone-loaded mixed polymeric micelles for nasal delivery.

Eur J Pharm Sci 2021 Jul 30:105960. Epub 2021 Jul 30.

Faculty of Pharmacy, Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, H-6720 Szeged, Hungary, Eötvös str. 6.. Electronic address:

Our study aimed to formulate a novel dexamethasone (DXM)-loaded, mixed polymeric micelle-based drug delivery system, focusing on the auspicious nose-to-brain pathway, as a key delivery route to treat central nervous system (CNS) associated diseases. Polymeric micelles might be a solution to deliver drugs to the place of action compared to conventional formulations. Due to low Z-average (89. Read More

View Article and Full-Text PDF

A review of attempts to develop the axenic culture of and genomics-based suggestions to achieve this elusive goal.

J Med Microbiol 2021 Jul;70(7)

Aix-Marseille Univ, IRD, APHM, MEPHI, Marseille, France.

To date, the axenic culture of remains a challenge in the field of microbiology despite countless attempts. Here, we conducted a comprehensive bibliographic analysis using several databases and search engines, namely Pubmed, Google scholar, Google, Web of Science and Scopus. Numerous unsuccessful empiric studies have been conducted and evaluated using as criteria dark-field microscopic observation of motile spiral shaped cells in the culture and virulence of the culture through rabbit infectivity. Read More

View Article and Full-Text PDF

Effect of cervical lesion centered access cavity restored with short glass fibre reinforced resin composites on fracture resistance in human mandibular premolars- an in vitro study.

J Mech Behav Biomed Mater 2021 Jun 26;122:104654. Epub 2021 Jun 26.

Department of Conservative Dentistry and Endodontics, Meenakshi Academy of Higher Education and Research University, Alapakkam Main Road, Maduravoyal, Chennai, 600095, India. Electronic address:

Aim: The aim of this study was to evaluate the fracture resistance of cervical lesion centered access cavity restored with short glass fibre reinforced resin materials in human mandibular premolars.

Materials And Methods: Sixty freshly extracted human mandibular premolars were collected and assigned to positive control group (G1- Intact teeth) and other experimental groups (G2, G3. G4, G5, G6), Traditional Access Cavity(TAC) and Cervical Lesion Centered Access Cavity(CLCAC). Read More

View Article and Full-Text PDF

Exploiting dentine matrix proteins in cell-free approaches for periradicular tissue engineering.

Tissue Eng Part B Rev 2021 Jul 26. Epub 2021 Jul 26.

University of Birmingham College of Medical and Dental Sciences, 150183, School of Dentistry, Institute of Clinical Sciences, 5 Mill Pool Way, Edgbaston, Birmingham, Birmingham, Birmingham, United Kingdom of Great Britain and Northern Ireland, B5 7EG.

The recent discovery of mesenchymal stem cells within periapical lesions (PL-MSC) has presented novel opportunities for managing periradicular diseases in adult teeth by way of enhancing tissue regeneration. This discovery coincides with the current paradigm shift towards biologically driven treatment strategies in endodontics, which have typically been reserved for non-vital immature permanent teeth. One such approach that shows promise is utilising local endogenous non-collagenous dentine extracellular matrix components (dECM) to recruit and upregulate the intrinsic regenerative capacity of PL-MSCs in situ. Read More

View Article and Full-Text PDF

The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells.

Int J Mol Sci 2021 Jul 12;22(14). Epub 2021 Jul 12.

Department for Bacteriology and Parasitology, Croatian Veterinary Institute, Savska Cesta 143, 10 000 Zagreb, Croatia.

The influence of cultivation on the expression pattern of canine adipose-derived mesenchymal stem cells (cAD-MSCs) surface markers, contributing to, among others, the promotion of growth, proliferation, differentiation and immunomodulatory mechanisms of an excellent therapeutic, is still unknown. To fill the gap, we investigated CD90, CD44, CD73, CD29, CD271, CD105, CD45 and CD14 patterns of expression at the protein level with flow cytometry and mRNA level using a real-time polymerase chain reaction array. Gentle variations of expression occurred during cultivation, along with increased CD90, CD44 and CD29 expression, low and decreasing CD271 and CD73 expression and a decrease of initially high CD105. Read More

View Article and Full-Text PDF

SPTAN1 Expression Predicts Treatment and Survival Outcomes in Colorectal Cancer.

Cancers (Basel) 2021 Jul 20;13(14). Epub 2021 Jul 20.

Department of Medicine, Biomedical Research Laboratory, University Hospital Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany.

Colorectal cancer (CRC) is a leading cause of cancer-related morbidity and mortality. In a cohort of 189 patients with CRC, we recently showed that expression of the cytoskeletal scaffolding protein non-erythroid spectrin αII (SPTAN1) was lower in advanced metastatic tumours. The aim of the present study was to clarify the association of intratumoural SPTAN1 expression levels with treatment and survival outcomes in patients with CRC. Read More

View Article and Full-Text PDF

TTAPE-Me dye is not selective to cardiolipin and binds to common anionic phospholipids non-specifically.

Biophys J 2021 Jul 16. Epub 2021 Jul 16.

Department of Biochemistry and Molecular Biology, McGovern Medical School at The University of Texas Health Science Center at Houston, TX 77030, USA; Department of Biochemistry, Biotechnology and Pharmacology, Kazan Federal University, Institute of Fundamental Medicine and Biology, Kazan, Russian Federation 420008. Electronic address:

Identification, visualization and quantitation of cardiolipin (CL) in biological membranes is of great interest due to important structural and physiological roles of this lipid. Selective fluorescent detection of CL using non-covalently bound fluorophore TTAPE-Me (1,1,2,2-tetrakis[4-(2-trimethylammonioethoxy)-phenylethene) has been recently proposed. However, this dye was only tested on wild-type mitochondria or liposomes containing negligible amounts of other anionic lipids, such as PG and PS. Read More

View Article and Full-Text PDF

RUNX1T1, a potential prognostic marker in breast cancer, is co-ordinately expressed with ERα, and regulated by estrogen receptor signalling in breast cancer cells.

Mol Biol Rep 2021 Jul 15. Epub 2021 Jul 15.

Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.

Background: RUNX1T1 is extensively studied in the context of AML1-RUNX1T1 fusion protein in acute myeloid leukemia. Little is known about the function of RUNX1T1 itself, although data on its function and regulation have begun to emerge from clinical, and in vitro studies. It is a putative tumor suppressor, whose expression is altered in a variety of solid tumors. Read More

View Article and Full-Text PDF

ORMDL1 is upregulated and associated with favorable outcomes in colorectal cancer.

Transl Oncol 2021 Jul 6;14(10):101171. Epub 2021 Jul 6.

Guangdong Institute of Gastroenterology, 26 Yuancun Er Heng Road, Guangzhou, Guangdong 510655, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, 26 Yuancun Er Heng Road, Guangzhou, Guangdong 510655, China; Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, 26 Yuancun Er Heng Road, Guangzhou, Guangdong 510655, China. Electronic address:

Background: The ORMDL1 gene is known as a crucial negative regulator of sphingolipid biogenesis. However, the ORMDL1 gene has rarely been studied in a tumor-related context. Therefore, its prognostic value and functional significance in colorectal cancer (CRC) remain to be explored. Read More

View Article and Full-Text PDF

Ocimum tenuiflorum mitigates iron-induced testicular toxicity via modulation of redox imbalance, cholinergic and purinergic dysfunctions, and glucose metabolizing enzymes activities.

Andrologia 2021 Jul 6:e14179. Epub 2021 Jul 6.

Department of Biochemistry, University of KwaZulu-Natal, Durban, South Africa.

Oxidative stress is a primary culprit in the pathophysiology of infertility conditions in males. This study investigated the effects of Ocimum tenuiflorum on redox imbalance, cholinergic and purinergic dysfunctions and glucose dysmetabolism in oxidative-mediated testicular toxicity using in vitro, ex vivo and in silico models. Induction of oxidative testicular injury was carried out by incubating normal testicular tissue with 0. Read More

View Article and Full-Text PDF

Design, synthesis, in vitro and in silico studies of some novel thiazole-dihydrofuran derivatives as aromatase inhibitors.

Bioorg Chem 2021 Jun 25;114:105123. Epub 2021 Jun 25.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskişehir, Turkey. Electronic address:

Aromatase inhibitors used against hormone-dependent breast cancer, especially in post-menopausal women, are very susceptible to the development of resistance due to their limited number and long-term use. In this study, it is aimed to obtain new aromatase inhibitors including thiazole and dihydrofuran ring systems. Synthesis of compounds (2a-2l) were performed according to literature methods. Read More

View Article and Full-Text PDF

Simultaneously boosting the conjugation, brightness and solubility of organic fluorophores by using AIEgens.

Chem Sci 2020 Jul 27;11(32):8438-8447. Epub 2020 Jul 27.

Department of Chemistry, The Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Institute for Advanced Study, Department of Chemical and Biological Engineering, Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong China

Organic near-infrared (NIR) emitters hold great promise for biomedical applications. Yet, most organic NIR fluorophores face the limitations of short emission wavelengths, low brightness, unsatisfactory processability, and the aggregation-caused quenching effect. Therefore, development of effective molecular design strategies to improve these important properties at the same time is a highly pursued topic, but very challenging. Read More

View Article and Full-Text PDF

New substrates and determinants for tRNA recognition of RNA methyltransferase DNMT2/TRDMT1.

RNA Biol 2021 Jun 10:1-15. Epub 2021 Jun 10.

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.

Methylation is a common post-transcriptional modification of tRNAs, particularly in the anticodon loop region. The cytosine 38 (C38) in tRNAs, such as tRNA, tRNA, tRNA, and tRNA, can be methylated by human DNMT2/TRDMT1 and some homologs found in bacteria, plants, and animals. However, the substrate properties and recognition mechanism of DNMT2/TRDMT1 remain to be explored. Read More

View Article and Full-Text PDF

Myocardial hypoxic stress mediates functional cardiac extracellular vesicle release.

Eur Heart J 2021 Jul;42(28):2780-2792

DZHK, Munich Partner Site, Ismaninger Str. 22, 81675 Munich, Germany.

Aims : Increased shedding of extracellular vesicles (EVs)-small, lipid bilayer-delimited particles with a role in paracrine signalling-has been associated with human pathologies, e.g. atherosclerosis, but whether this is true for cardiac diseases is unknown. Read More

View Article and Full-Text PDF

Rational design, synthesis and testing of novel tricyclic topoisomerase inhibitors for the treatment of bacterial infections part 2.

RSC Med Chem 2020 Dec 18;11(12):1379-1385. Epub 2020 Sep 18.

Redx Anti-Infectives Ltd Alderley Park Macclesfield SK10 4TG Cheshire UK.

Building on our previously-reported novel tricyclic topoisomerase inhibitors (NTTIs), we disclose the discovery of REDX07965, which has an MIC of 0.5 μg mL against , favourable pharmacokinetic properties, selectivity human topoisomerase II and an acceptable toxicity profile. The results herein validate a rational design approach to address the urgent unmet medical need for novel antibiotics. Read More

View Article and Full-Text PDF
December 2020

Bi-directional regulation functions of lanthanum-substituted layered double hydroxide nanohybrid scaffolds activating osteogenesis and inhibiting osteoclastogenesis for osteoporotic bone regeneration.

Theranostics 2021 3;11(14):6717-6734. Epub 2021 May 3.

The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.

Osteoporotic patients suffer symptoms of excessive osteoclastogenesis and impaired osteogenesis, resulting in a great challenge to treat osteoporosis-related bone defects. Based on the positive effect of rare earth elements on bone metabolism and bone regeneration, we try to prove the hypothesis that the La dopants in lanthanum-substituted MgAl layered double hydroxide (La-LDH) nanohybrid scaffolds simultaneously activate osteogenesis and inhibit osteoclastogenesis. A freeze-drying technology was employed to construct La-LDH nanohybrid scaffolds. Read More

View Article and Full-Text PDF

Structural characterisation and bioactivity of polysaccharides isolated from fermented Dendrobium officinale.

J Sci Food Agric 2021 Jun 6. Epub 2021 Jun 6.

School of Food Science and Engineering, University of Hainan, Haikou, China.

Background: A polysaccharide was purified in this study, which was acquired from the fermentation broth of Dendrobium officinale Kimura et Migo. We aimed to investigate the structural features and bioactivity of this polysaccharide.

Results: The polysaccharide was purified and the main polysaccharide fraction (i. Read More

View Article and Full-Text PDF

A practical guide for evaluating the osteoimmunomodulatory properties of biomaterials.

Acta Biomater 2021 08 2;130:115-137. Epub 2021 Jun 2.

Ortholab, Department of Surgical Sciences-Orthopaedics, Uppsala University, 751 85 Uppsala, Sweden.

Biomaterials offer a promising approach to repair bone defects. Whereas traditional studies predominantly focused on optimizing the osteogenic capacity of biomaterials, less focus has been on the immune response elicited by them. However, the immune and skeletal systems extensively interact, a concept which is referred to as 'osteoimmunology'. Read More

View Article and Full-Text PDF

Addition of lactoferrin and substance P in a chitin/PLGA-CaSO hydrogel for regeneration of calvarial bone defects.

Mater Sci Eng C Mater Biol Appl 2021 Jul 6;126:112172. Epub 2021 May 6.

Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi-682041, India. Electronic address:

Calcium-based injectable hydrogels with various bioactive active molecules possess a great potential for bone regeneration. Herein, we have synthesized a chitin-PLGA-calcium sulfate hydrogel (CSG) containing bioactive molecules - lactoferrin (LF) and substance P (SP). SEM and XRD analysis revealed that CS crystal growth was altered with the addition of LF. Read More

View Article and Full-Text PDF

Current Knowledge on Functionality and Potential Therapeutic Uses of Donkey Milk.

Animals (Basel) 2021 May 13;11(5). Epub 2021 May 13.

Department of Veterinary Science, University of Pisa, 56124 Pisa, Italy.

The increase of knowledge on the composition of donkey milk has revealed marked similarities to human milk, which led to a growing number of investigations focused on testing the potential effects of donkey milk in vitro and in vivo. This paper examines the scientific evidence regarding the beneficial effects of donkey milk on human health. Most clinical studies report a tolerability of donkey milk in 82. Read More

View Article and Full-Text PDF

Modulation of γ-Secretase Activity by a Carborane-Based Flurbiprofen Analogue.

Molecules 2021 May 11;26(10). Epub 2021 May 11.

Institut für Anorganische Chemie, Universität Leipzig, Johannisallee 29, D-04103 Leipzig, Germany.

All over the world, societies are facing rapidly aging populations combined with a growing number of patients suffering from Alzheimer's disease (AD). One focus in pharmaceutical research to address this issue is on the reduction of the longer amyloid-β (Aβ) fragments in the brain by modulation of γ-secretase, a membrane-bound protease. -Flurbiprofen (tarenflurbil) was studied in this regard but failed to show significant improvement in AD patients in a phase 3 clinical trial. Read More

View Article and Full-Text PDF

Fertility preservation opportunities for cancer patients in Malaysia.

Med J Malaysia 2021 05;76(3):417-418

Universiti Putra Malaysia, Faculty of Medicine and Health Sciences, Serdang, Selangor, Malaysia.

Fertility preservation is significant for oncology patients to maintain their ability to start a family when they are ready. Onco-fertility, as a discipline, exists at the intersection of oncology and reproductive medicine that safeguards and expands the fertility options for cancer survivors, by facilitating early intervention and suitable treatment with favourable outcomes. Successful fertility preservation requires a comprehensive networking among the gynaecologists, oncologists, pathologists, imaging and other specialists, involved in diagnosing and treating cancer in the reproductive age group. Read More

View Article and Full-Text PDF

In vitro and in vivo interaction of caspofungin with isavuconazole against Candida auris planktonic cells and biofilms.

Med Mycol 2021 May 22. Epub 2021 May 22.

Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98., 4032 Debrecen, Hungary.

The in vitro and in vivo efficacy of caspofungin was determined in combination with isavuconazole against Candida auris. Drug-drug interactions were assessed utilising the fractional inhibitory concentration indices (FICIs), the Bliss independence model and an immunocompromised mouse model. Median planktonic minimum inhibitory concentrations (pMICs) of 23 C. Read More

View Article and Full-Text PDF

Water jet-assisted lipoaspiration and Sepax cell separation system for the isolation of adipose stem cells with high adipogenic potential.

J Plast Reconstr Aesthet Surg 2021 Mar 29. Epub 2021 Mar 29.

Department of Integrative Medical Biology, Umeå University, Sweden.

Introduction: Water jet-assisted liposuction has gained popularity due to favourable fat grafting outcomes. In this study, we compared stem cells obtained from fat isolated with manual or the water jet-assisted procedure.

Methods: Liposuction of abdominal fat was performed using the two methods on each donor (n = 10). Read More

View Article and Full-Text PDF

Pharmacokinetics, metabolism and off-target effects in the rat of 8-[(1H- benzotriazol-1-yl)amino]octanoic acid, a selective inhibitor of human cytochrome P450 4Z1: -oxidation as a potential augmenting pathway for inhibition.

Xenobiotica 2021 Aug 11;51(8):901-915. Epub 2021 Jun 11.

Department of Medicinal Chemistry, School of Pharmacy, University of Washington, Seattle, WA, USA.

8-[(1H-1,2,3-benzotriazol-1-yl)amino]octanoic acid (8-BOA) was recently identified as a selective and potent mechanism-based inactivator (MBI) of breast cancer-associated CYP4Z1 and exhibited favourable inhibitory activity thus meriting characterization.The pharmacokinetics and metabolism of 8-BOA in rats was examined after a single IV bolus dose of 10 mg/kg. A biphasic time-concentration profile resulted in relatively low clearance and a prolonged elimination half-life. Read More

View Article and Full-Text PDF

Toxicological and anti-tumor effects of a linden extract ( Scop.) in a HPV16-transgenic mouse model.

Food Funct 2021 May;12(9):4005-4014

Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal.

Tilia platyphyllos Scop. is a popular broad-leaved tree, native to Central and Southern Europe. Hydroethanolic extracts rich in phenolic compounds obtained from T. Read More

View Article and Full-Text PDF

Role of calcium phosphate and bioactive glass coating on in vivo bone healing of new Mg-Zn-Ca implant.

J Mater Sci Mater Med 2021 May 7;32(5):55. Epub 2021 May 7.

Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, India.

Present investigation focuses on development and detailed characterization of a new Mg alloy sample (BM) with and without coating of hydroxyapatite (BMH) and bioactive glass (BMG) by air plasma spray method. After detailed mechano-physico-chemical characterization of powders and coated samples, electrochemical corrosion and SBF immersion tests were carried out. Detailed in vitro characterizations for cell viability were undertaken using MG-63 cell line followed by in vivo tests in rabbit model for studying bone healing up to 60 days. Read More

View Article and Full-Text PDF

Assisted reproductive technology outcomes in women with a chronic viral disease.

AIDS 2021 06;35(7):1073-1081

Université de Paris, Faculté de Médecine.

Objective: The aim of this study was to evaluate the cumulative live birth rate in women undergoing in-vitro fertilization/intracytoplasmic-sperm-injection (IVF/ICSI) according to the type of chronic viral infection [HIV, hepatitis-B virus (HBV) and hepatitis-C virus (HCV)].

Design: A cohort study.

Setting: A tertiary-care university hospital. Read More

View Article and Full-Text PDF

Assessment of the biological activity of fish muscle protein hydrolysates using in vitro model systems.

Food Chem 2021 Oct 20;359:129852. Epub 2021 Apr 20.

School of Food and Nutritional Science, University College Cork, T12 YN60 Cork, Ireland. Electronic address:

The generation of biologically active fish protein hydrolysates (FPH) is a useful technique to produce value-added products with potential application in the functional food and nutraceutical industries. Fish muscle is an attractive substrate for the production of protein hydrolysates due to its rich protein content, containing 15-25% of total fish protein. This paper reviews the production of protein hydrolysates from fish muscle, most commonly via enzymatic hydrolysis, and their subsequent bioactivities including anti-obesity, immunomodulatory, antioxidant, angiotensin I-converting enzyme (ACE)-inhibitory, anti-microbial, and anti-cancer activities as measured by in vitro testing methods. Read More

View Article and Full-Text PDF
October 2021

Cross-talk between motor neurons and myotubes via endogenously secreted neural and muscular growth factors.

Physiol Rep 2021 Apr;9(8):e14791

Musculoskeletal Science & Sports Medicine Research Centre, Department of Life Sciences, Manchester Metropolitan University, Manchester, UK.

Neuromuscular junction (NMJ) research is vital to advance the understanding of neuromuscular patho-physiology and development of novel therapies for diseases associated with NM dysfunction. In vivo, the micro-environment surrounding the NMJ has a significant impact on NMJ formation and maintenance via neurotrophic and differentiation factors that are secreted as a result of cross-talk between muscle fibers and motor neurons. Recently we showed the formation of functional NMJs in vitro in a co-culture of immortalized human myoblasts and motor neurons from rat-embryo spinal-cord explants, using a culture medium free from serum and neurotrophic or growth factors. Read More

View Article and Full-Text PDF