3,207 results match your criteria facilitated mrna

Size effects of polystyrene microplastics on the accumulation and toxicity of (semi-)metals in earthworms.

Environ Pollut 2021 Sep 16;291:118194. Epub 2021 Sep 16.

Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China. Electronic address:

Microplastics (MPs) are plastic fragments less than 5 mm, which may have adverse impacts on organisms. In this study, we investigated the impacts and mechanisms of polystyrene MPs (10 μm and 100 μm) and nanoplastics (NPs, 100 nm) with different concentrations (10 mg/kg and 100 mg/kg) in soil on the uptake of metal Cd and semi-metal As in earthworms, Eisenia fetida. MPs facilitated the accumulation of (semi-)metals via damaging the integrity of earthworm intestine, and earthworms accumulated more (semi-)metals in MP treatment groups than NP treatment groups, especially in group of 100 mg/kg of 10 μm MP with concentrations of 1. Read More

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September 2021

Circular RNA repertoires are associated with evolutionarily young transposable elements.

Elife 2021 Sep 20;10. Epub 2021 Sep 20.

Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.

Circular RNAs (circRNAs) are found across eukaryotes and can function in post-transcriptional gene regulation. Their biogenesis through a circle-forming backsplicing reaction is facilitated by reverse-complementary repetitive sequences promoting pre-mRNA folding. Orthologous genes from which circRNAs arise, overall contain more strongly conserved splice sites and exons than other genes, yet it remains unclear to what extent this conservation reflects purifying selection acting on the circRNAs themselves. Read More

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September 2021

Effects of Total Dietary Fiber on Cecal Microbial Community and Intestinal Morphology of Growing White Pekin Duck.

Front Microbiol 2021 1;12:727200. Epub 2021 Sep 1.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

The current study was to investigate the effects of total dietary fiber (TDF) on growth performance, cecal structure, cecal microbial community, and short-chain fatty acids (SCFAs) profiles in the cecum of growing White Pekin ducks. A total of 108 male Pekin ducks of 14-days-old were randomly allocated and fed diets containing 12.4, 14. Read More

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September 2021

Fluoride regulates chondrocyte proliferation and autophagy via PI3K/AKT/mTOR signaling pathway.

Chem Biol Interact 2021 Sep 15:109659. Epub 2021 Sep 15.

Department of Environmental Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province, PR China. Electronic address:

Fluorine is an essential trace element for human health. However, excessive fluoride intake causes skeletal fluorosis which affects cartilage development. Fluoride inhibited chondrocyte proliferation which is the initial and critical step of endochondral ossification, but the underlying mechanism has not been clearly illustrated. Read More

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September 2021

METTL3 induces PLX4032 resistance in melanoma by promoting mA-dependent EGFR translation.

Cancer Lett 2021 Sep 13;522:44-56. Epub 2021 Sep 13.

College of Pharmacy, Chosun University, Gwangju, 61452, Republic of Korea. Electronic address:

Acquired resistance often limits therapeutic efficacy of the BFAF (V600E) kinase inhibitor PLX4032 in patients with advanced melanoma. Epitranscriptomic modification of mRNAs by N-methyladenosine (mA) modification contributes to melanoma pathogenesis; however, its role in acquired PLX4032 resistance remains unexplored. Here, we showed that mA methyltransferase METTL3 expression is upregulated in A375R cells, a PLX4032-resistant subline of A375 melanoma cells, compared with the parental cells. Read More

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September 2021

Camostat mesilate inhibits pro-inflammatory cytokine secretion and improves cell viability by regulating MFGE8 and HMGN1 in lipopolysaccharide-stimulated DF-1 chicken embryo fibroblasts.

PeerJ 2021 26;9:e12053. Epub 2021 Aug 26.

Henan Key Laboratory of Farm Animal Breeding and Nutritional Regulation, Institute of Animal Husbandry and Veterinary Medicine, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China.

Camostat mesilate (CM) possesses potential anti-viral and anti-inflammatory activities. However, it remains unknown whether CM is involved in lipopolysaccharide (LPS)-mediated inflammatory responses and cell injury. In this project, differentially expressed proteins (DEPs, fold change ≥ 1. Read More

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VIRMA contributes to non-small cell lung cancer progression via N-methyladenosine-dependent DAPK3 post-transcriptional modification.

Cancer Lett 2021 Sep 11. Epub 2021 Sep 11.

Department of Respiratory Disease, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, PR China. Electronic address:

N-methyladenosine (m6A) has been reported to be abnormally expressed in non-small cell lung cancer (NSCLC), and plays a vital role in regulation of cell proliferation, invasion and metastasis. Vir-Like m6A methyltransferase associated (VIRMA, also called KIAA1429) has not been well studied in NSCLC. Thus, in this study, we investigated the biological impact and underlying mechanism of VIRMA in NSCLC. Read More

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September 2021

Protective effect of MitoQ on oxidative stress-mediated senescence of canine bone marrow mesenchymal stem cells via activation of the Nrf2/ARE pathway.

In Vitro Cell Dev Biol Anim 2021 Aug 13;57(7):685-694. Epub 2021 Sep 13.

College of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, China.

The destruction of biological activity such as senescence and apoptosis caused by oxidative stress could play a pivotal role in the poor therapeutic efficiency of bone marrow mesenchymal stem cells (BMSCs) transplantation. Mitoquinone (MitoQ) has a highly effective mitochondrial antioxidant effect, and has been widely used in many oxidative damage models. This study aimed to investigate the protective effect of MitoQ on the oxidative stress-mediated senescence of canine BMSCs and the underlying mechanism. Read More

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IGF2BP1 promotes the liver cancer stem cell phenotype by regulating MGAT5 mRNA stability via m6A RNA methylation.

Stem Cells Dev 2021 Sep 13. Epub 2021 Sep 13.

The First Affiliated Hospital of Chongqing Medical University, 117972, Department of Gastroenterology, Chongqing, China;

The aim of this study was to elucidate the mechanism of action of the insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) on the phenotype of the liver cancer stem cells (LCSCs). To gain insight into the mechanism of action of the IGF2BP1 on LCSCs, the IGF2BP1 shRNA sequences were transfected into HCC cells. The LCSC phenotypes were measured by stemness gene expressions, spheroid formations, percentages of the CD133+ cells, colony formations, and tumorigeneses in vivo. Read More

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September 2021

The RaPID Platform for the Discovery of Pseudo-Natural Macrocyclic Peptides.

Acc Chem Res 2021 Sep 10;54(18):3604-3617. Epub 2021 Sep 10.

Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo 113-0033, Japan.

ConspectusAlthough macrocyclic peptides bearing exotic building blocks have proven their utility as pharmaceuticals, the sources of macrocyclic peptide drugs have been largely limited to mimetics of native peptides or natural product peptides. However, the recent emergence of technologies for discovering bioactive peptides has led to their reconceptualization as a promising therapeutic modality. For the construction and screening of libraries of such macrocyclic peptides, our group has devised a platform to conduct affinity-based selection of massive libraries (>10 unique sequences) of expressed macrocyclic peptides, which is referred to as the random nonstandard peptides integrated discovery (RaPID) system. Read More

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September 2021

MicroRNA-301b-3p facilitates cell proliferation and migration in colorectal cancer by targeting HOXB1.

Bioengineered 2021 Dec;12(1):5839-5849

Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.

Previous studies revealed that miR-301b-3p was essential to the onset and development of several cancers, but the implied functions of miR-301b-3p in colorectal cancer (CRC) remained largely unclear. The current study is aimed to exploring the potential roles and possible mechanism of miR-301b-3p in CRC. The abundance of miR-301b-3p and HOXB1 in CRC clinical specimens and cell lines was verified using RT-qPCR. Read More

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December 2021

Downregulation of Filamin a Expression in the Aorta Is Correlated With Aortic Dissection.

Front Cardiovasc Med 2021 13;8:690846. Epub 2021 Aug 13.

Division of Cardiothoracic and Vascular Surgery, Sino-Swiss Heart-Lung Transplantation Institute, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Filamins (FLNs) are actin cross-linking proteins, and as scaffolding proteins, FLNs are closely associated with the stabilization of the cytoskeleton. Nevertheless, the biological importance of FLNs in aortic dissection (AD) has not been well-elucidated. In this study, we first reanalyzed datasets downloaded from the Gene Expression Omnibus (GEO) database, and we found that in addition to the extracellular matrix, the actin cytoskeleton is a key structure associated with AD. Read More

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RecQ-like helicase 4 (RECQL4) exacerbates resistance to oxaliplatin in colon adenocarcinoma via activation of the PI3K/AKT signaling pathway.

Bioengineered 2021 Dec;12(1):5859-5869

Department of Colorectal Surgery, Jiangsu Provincial People's Hospital, China.

Oxaliplatin (OXA) resistance is a great challenge for colon adenocarcinoma (COAD) chemotherapy. The promoting role of RecQ-Like Helicase 4 (RECQL4) in chemoresistance to platinum-based drugs has been identified, whereas the effect and specific mechanism of RECQL4 in regulating OXA resistance within COAD have not been explicated yet. In this work, RECQL4 mRNA expression was detected by RT-qPCR. Read More

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December 2021

Whole-genome sequencing facilitates patient-specific quantitative PCR-based minimal residual disease monitoring in acute lymphoblastic leukaemia, neuroblastoma and Ewing sarcoma.

Br J Cancer 2021 Sep 1. Epub 2021 Sep 1.

Children's Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney, NSW, Australia.

Background: Minimal residual disease (MRD) measurement is a cornerstone of contemporary acute lymphoblastic leukaemia (ALL) treatment. The presence of immunoglobulin (Ig) and T cell receptor (TCR) gene recombinations in leukaemic clones allows widespread use of patient-specific, DNA-based MRD assays. In contrast, paediatric solid tumour MRD remains experimental and has focussed on generic assays targeting tumour-specific messenger RNA, methylated DNA or microRNA. Read More

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September 2021

Repair of APOBEC3G-mutated retroviral DNA is facilitated by the host enzyme uracil DNA glycosylase 2.

J Virol 2021 Sep 1:JVI0124421. Epub 2021 Sep 1.

Department of Microbiology and Immunology, University of Illinois at Chicago College of Medicine, Chicago IL 60612.

Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3 (APOBEC3) proteins are critical for the control of infection by retroviruses. These proteins deaminate cytidines in negative strand DNA during reverse transcription, leading to G to A changes in coding strands. Uracil DNA glycosylase (UNG) is a host enzyme that excises uracils in genomic DNA, which the base excision repair machinery then repairs. Read More

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September 2021

Long noncoding RNA SNHG4 remits lipopolysaccharide-engendered inflammatory lung damage by inhibiting METTL3 - Mediated mA level of STAT2 mRNA.

Mol Immunol 2021 Aug 25;139:10-22. Epub 2021 Aug 25.

Neonatal Department, Children's Hospital Affiliate to Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710003, China. Electronic address:

Background: Emerging evidence suggests that long non coding RNA (lncRNA) small nucleolar RNA host gene 4 (SNHG4) has become a new insight into lipopolysaccharide (LPS)-induced microglia inflammation, its role in neonatal pneumonia (NP) remains to be largely unrevealed.

Methods: RT-qPCR was used to determine the expression of SNHG4 and METTL3 in the serum from NP patients and normal volunteers, as well as in LPS treated-WI-38 cells. The SNHG4 overexpression vector (pcDNA-SNHG4) was transfected into LPS-treated cells. Read More

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PRMT5>regulates ovarian follicle development by facilitating translation.

Elife 2021 Aug 27;10. Epub 2021 Aug 27.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Protein arginine methyltransferase 5 () is the major type II enzyme responsible for symmetric dimethylation of arginine. Here, we found PRMT5 was expressed at high level in ovarian granulosa cells of growing follicles. Inactivation of in granulosa cells resulted in aberrant follicle development and female infertility. Read More

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Propamocarb exposure has the potential to accelerate the formation of atherosclerosis in both WT and ApoE mice accompanied by gut microbiota dysbiosis.

Sci Total Environ 2021 Aug 12;800:149602. Epub 2021 Aug 12.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, Zhejiang, China. Electronic address:

Propamocarb is a systemic carbamate fungicide used to fight diseases. The effect of propamocarb on the formation of atherosclerosis was evaluated in wild-type (WT) and ApoE knockout (ApoE) mice. C57BL/6 J WT mice were fed control diet or high-fat diet (HFD) with 20 mg/L propamocarb in drinking water for 24 weeks. Read More

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ERCC6L promotes cell growth and metastasis in gastric cancer through activating NF-κB signaling.

Aging (Albany NY) 2021 08 23;13(16):20218-20228. Epub 2021 Aug 23.

Department of General Surgery, Taizhou People's Hospital, The Fifth Affiliated Hospital of Nantong University, Taizhou 225300, China.

ERCC6L has been reported to act as a potential oncogenic protein in various cancers. However, the role of ERCC6L in the progression of gastric cancer (GC) remains to be elucidated. Herein, we aimed to assess the clinical significance, the role, and the underlying mechanism of ERCC6L in GC progression. Read More

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Long non-coding RNA FOXD3 antisense RNA 1 augments anti-estrogen resistance in breast cancer cells through the microRNA-363/ trefoil factor 1/ phosphatidylinositol 3-kinase/protein kinase B axis.

Bioengineered 2021 Dec;12(1):5266-5278

Department of Medical Oncology, the Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang, P.R. China.

Long non-coding RNA (lncRNA) FOXD3 antisense RNA 1 (FOXD3-AS1) has been reported to participate in multiple processes that contribute toward the development of cancer. The present study aimed to explore the effect of lncRNA FOXD3-AS1 on anti-estrogen resistance in breast cancer (BC) cells. FOXD3-AS1 was found to be highly expressed in BC cell lines. Read More

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December 2021

TMEM100 Modulates TGF- Signaling Pathway to Inhibit Colorectal Cancer Progression.

Gastroenterol Res Pract 2021 11;2021:5552324. Epub 2021 Aug 11.

Department of Colorectal and Anal Surgery, Lishui Municipal Central Hospital, Affiliated Lishui Hospital of Zhejiang University, Lishui 323000, China.

Objectives: This study investigated the functional mechanism of transmembrane protein 100 (TMEM100) as a tumor inhibitor gene in CRC cells and offered a reference for the treatment of CRC.

Methods: The mRNA expression data of CRC were acquired from the TCGA database to mine differentially expressed mRNAs. The role of TMEM100 in the progression of CRC cells was evaluated by MTT, colony formation, scratch healing, and Transwell assays. Read More

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Role of nitric oxide on zymosan-induced inhibition of crop emptying in chicks.

Comp Biochem Physiol A Mol Integr Physiol 2021 Nov 19;261:111057. Epub 2021 Aug 19.

Department of Agrobiological Science, Faculty of Agriculture, Ehime University, Matsuyama 790-8566, Ehime, Japan. Electronic address:

Zymosan, a component of yeast cell walls, reduces feed passage through the digestive tract in chicks (Gallus gallus), although the mechanism mediating this effect is poorly understood. Nitric oxide (NO) is associated with a variety of biological actions including effects on the immune system. In addition, it has been suggested that NO is involved in relaxation of the digestive tract and affects feed passage in mammals. Read More

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November 2021

Lysophosphatidate Promotes Sphingosine 1-Phosphate Metabolism and Signaling: Implications for Breast Cancer and Doxorubicin Resistance.

Cell Biochem Biophys 2021 Aug 20. Epub 2021 Aug 20.

Department of Biochemistry, University of Alberta, Edmonton, AB, T6G 2S2, Canada.

Lysophosphatidate (LPA) and sphingosine 1-phosphate (S1P) promote vasculogenesis, angiogenesis, and wound healing by activating a plethora of overlapping signaling pathways that stimulate mitogenesis, cell survival, and migration. As such, maladaptive signaling by LPA and S1P have major effects in increasing tumor progression and producing poor patient outcomes after chemotherapy and radiotherapy. Many signaling actions of S1P and LPA are not redundant; each are vital in normal physiology and their metabolisms differ. Read More

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Effect and mechanism of propranolol on promoting osteogenic differentiation and early implant osseointegration.

Int J Mol Med 2021 Oct 20;48(4). Epub 2021 Aug 20.

Department of Oral Implantology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China.

The present study aimed to investigate the effect of β‑receptor blocker propranolol on early osseointegration of pure titanium implants and the underlying molecular regulatory mechanisms. An implant osseointegration model using the tibial metaphysis of New Zealand rabbits was established. The rabbits were divided into control and low‑, medium‑ and high‑dose propranolol groups. Read More

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October 2021

ORC6, Negatively Regulated by miR-1-3p, Promotes Proliferation, Migration, and Invasion of Hepatocellular Carcinoma Cells.

Front Cell Dev Biol 2021 29;9:652292. Epub 2021 Jul 29.

Department of Laboratory, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: In recent years, microRNA-1-3p (miR-1-3p) has been linked to the progression of multiple cancers, whereas little is known about its role in hepatocellular carcinoma (HCC). Herein, we investigated the function of miR-1-3p in HCC, and its regulatory function on origin recognition complex subunit 6 (ORC6).

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for detecting the expression levels of miR-1-3p and ORC6 mRNA in HCC samples and cell lines. Read More

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Microcyst fluid promotes the migration and invasion of fibroblasts in the adventitial layer of alveolar echinococcosis.

Acta Trop 2021 Aug 10;223:106084. Epub 2021 Aug 10.

Research Center for High Altitude Medicine, Qinghai University, Xining, China; Qinghai Key Laboratory of Science and Technology for High Altitude Medicine, Xining, China. Electronic address:

Alveolar echinococcosis (AE) caused by Echinococcus multilocularis (E. multilocularis), characterized by lesions composed of an aggregate of microcysts embedded in a granulomatous host's reaction. The periphery of parasite granulomas often additionally displays fibrotic reactions of varying intensity, in which E. Read More

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Caffeine-induced inversion of prostaglandin E effects on hepatic stellate cell activation.

Biomed Pharmacother 2021 Oct 10;142:111989. Epub 2021 Aug 10.

Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka City, Shizuoka 422-8526, Japan.

Background And Aims: Liver inflammation leads to the activation of hepatic stellate cells (HSCs), resulting in the development of liver fibrosis. The present study aimed to investigate the effects of prostaglandin E (PGE), which is biosynthesized by Kupffer cells, hepatocytes, and HSCs during inflammation, on HSC activation, including its combinatory effect with caffeine.

Methods: HSCs isolated from mice were activated by culturing in a medium supplemented with 10% fetal bovine serum for 7 days on plastic plates. Read More

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October 2021

Enterotoxigenic Bacteroidesfragilis Promotes Intestinal Inflammation and Malignancy by Inhibiting Exosome-Packaged miR-149-3pf.

Gastroenterology 2021 Aug 8. Epub 2021 Aug 8.

State Key Laboratory for Oncogenes and Related Genes, Shanghai, China; Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, Shanghai, China; Division of Gastroenterology and Hepatology, Shanghai Cancer Institute, Shanghai, China; Shanghai Institute of Digestive Disease, Shanghai, China; Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Background & Aims: Enterotoxigenic Bacteroides fragilis (ETBF) is strongly associated with the occurrence of inflammatory bowel disease (IBD), colitis-associated colorectal cancer, and colorectal cancer (CRC). However, the mechanism of ETBF-induced intestinal inflammation and tumorigenesis remains unclear.

Methods: microRNA sequencing was used to detect the differentially expressed microRNAs in both ETBF-treated cells and exosomes derived from ETBF-inoculated cells. Read More

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MMP1 regulated by NEAT1/miR-361-5p axis facilitates the proliferation and migration of cutaneous squamous cell carcinoma via the activation of Wnt pathway.

Cancer Biol Ther 2021 Jun 7;22(5-6):381-391. Epub 2021 Aug 7.

Department of Burn and Plastic Surgery, Shenzhen Longhua District Central Hospital, No. 187 Guanlan Avenue, Longhua District, Shenzhen, 518110, Guangdong, China.

Cutaneous squamous cell carcinoma (CSCC) is one of the most malignant tumors worldwide. It has been validated that matrix metallopeptidase 1 (MMP1) expression was obviously up-regulated in CSCC tissues. However, its specific role in CSCC is still unclear. Read More

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CTR9-mediated JAK2/STAT3 pathway promotes the proliferation, migration, and invasion of human glioma cells.

J Clin Lab Anal 2021 Sep 8;35(9):e23943. Epub 2021 Aug 8.

Department of neurosurgery, the Affiliated Brain Hospital of Nanjing Medical University, Nanjing Medical University, Nanjing, China.

Background: CTR9 (Cln three requiring 9) has been reported to be implicated in protein modification and oncogenesis of several human cancers. However, the protein expression and mechanism of CTR9 in glioma progression remain unclear.

Methods: We analyzed mRNA expression of CTR9 and CTR9-related survival curves in the public database. Read More

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September 2021