Molecules 2019 Feb 11;24(3). Epub 2019 Feb 11.
Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Seven diterpene lactones, andrographolide (), isoandrographolide (), neo-andrographolide (), 14-deoxy-11,12-didehydroandrographolide (), 14-deoxyandrographiside (), 14-deoxy-11,12-didehydroandrographiside (), 3,14-dideoxyandrographolide (), and three flavones, andrographidine C (), andrographidine A (), 5-hydroxy-7,8-dimethoxyflavanone () have been successfully and efficiently isolated from using an off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) method for the first time. For the first dimension HSCCC separation, petroleum ether-ethyl acetate-methanol-water 3:7:5:5 (/) was employed to isolate 14.4 mg of compound , 3. Read More