310,036 results match your criteria extracts

Novel alimentary pasta made of chickpeas has an important allergenic content that is altered by boiling in a different manner than chickpea seeds.

Food Chem 2022 Jun 27;395:133586. Epub 2022 Jun 27.

Department of Allergy, Research Institute Hospital 12 de Octubre (imas12), Avenida de Córdoba s/n, 28041 Madrid, Spain. Electronic address:

Alimentary pasta made of chickpeas has been recently introduced in the market. The novelty and presentation of this food can have a confounding effect on chickpea allergic patients and can pose a risk to them. The allergenic content of novel alimentary chickpea pasta in comparison with regular chickpea seeds has not been analyzed so far. Read More

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Multi-residue analytical method for detecting pesticides, veterinary drugs, and mycotoxins in feed using liquid- and gas chromatography coupled with mass spectrometry.

J Chromatogr A 2022 Jun 23;1676:463257. Epub 2022 Jun 23.

Experiment Research Institute, National Agricultural Products Quality Management Service, 141, Yongjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, South Korea. Electronic address:

Modified QuEChERS and triple quadrupole mass spectrometry (LC and GC-MS/MS) technology were used to sequentially analyze pesticides, veterinary drugs, and mycotoxins in feed. In order to analyze the harmful substances that may remain or occur in the feed, we performed optimization experiments for sample preparation and LC-MS/MS and GC-MS/MS conditions. Optimized sample preparation involves extracting 5 g of sample with 15 mL of 0. Read More

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Sperm-borne proteins improve rabbit cloning efficiency via regulating embryonic cleavage and epigenetics.

Proteomics 2022 Jul 2:e2200020. Epub 2022 Jul 2.

Laboratory Animal Center, Xi'an Jiaotong University Health Science Centre, Xi'an, Shaanxi, China.

Somatic cell nuclear transfer (SCNT) shows great application value in the generation of transgenic animals, protection of endangered species, and therapeutic cloning. However, the cloning efficiency is still very low, which greatly restricts its application. Compared to fertilized embryos, cloned embryos lack the sperm proteins, which are considered to play an important role in embryonic development. Read More

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The clinical cross-reactivity and immunological cross-antigenicity of wheat and barley.

Allergol Int 2022 Jun 28. Epub 2022 Jun 28.

Department of Allergy, Allergy and Immunology Center, Aichi Children's Health and Medical Center, Obu, Japan; Department of Pediatrics, Yokohama City University Graduate School of Medicine, Yokohama, Japan; Department of Comprehensive Pediatric Medicine, Nagoya University Graduate School of Medicine, Obu, Japan. Electronic address:

Background: Some patients with wheat allergy have been reported to show clinical cross-reactivity to barley. However, it is not clear whether the development of barley allergy in patients with wheat allergy is due to cross-antigenicity between wheat and barley. This study aimed to determine the clinical cross-reactivity and immunological cross-antigenicity of wheat and barley. Read More

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Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.

Phytochem Anal 2022 Jul 1. Epub 2022 Jul 1.

Faculty of Pharmacy, Pharmaceutical Sciences Department, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.

Introduction: Coffea arabica L. leaves are considered a by-product of the coffee industry however they are sources of several bioactive compounds.

Objectives: This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL). Read More

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Network pharmacology provides a systematic approach to understanding the treatment of ischemic heart diseases with traditional Chinese medicine.

Phytomedicine 2022 Jun 12;104:154268. Epub 2022 Jun 12.

Faculty of Chinese Medicine and State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao, P R China; Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, Macau University of Science and Technology, Taipa, Macao, P R China; Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, P R China; Zhuhai Hospital of Integrated Traditional Chinese and Western Medicine, Zhuhai, Guangdong Province, P R China; Guangdong Provincial Hospital of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, State Key Laboratory of Dampness Syndrome of Chinese Medicine, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, P R China. Electronic address:

Background: The field of network pharmacology showed significant development. The concept of network pharmacology has many similarities to the philosophy of traditional Chinese medicine (TCM), making it suitable to understand the action mechanisms of TCM in treating complex diseases, such as ischemic heart diseases (IHDs).

Purpose: This review summarizes the representative applications of network pharmacology in deciphering the mechanism underlying the treatment of IHDs with TCM. Read More

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Molecular network-guided chemical profile and mass spectrometry, volatile compounds and antimicrobial activity of Scaptotrigona depilis propolis.

Rapid Commun Mass Spectrom 2022 Jul 1:e9348. Epub 2022 Jul 1.

Universidade Federal de Roraima-Programa de Pós-graduação em Biodiversidade e Biotecnologia da Amazônia, Boa Vista, RR, Brazil.

Rationale: Propolis has a great diversity in its composition due to numerous factors, therefore each study is an important contribution to the knowledge of its composition and biological action. The objective of this study was to determine the chemical profile and biological activity of propolis produced by Scaptotrigona depilis.

Methods: Extracts with 70% ethanol (EPE70) and with cereal alcohol (CAPE) were elaborated, and then characterized using UHPLC-ESI(+)-MS/MS. Read More

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Insulin sensitizer and antihyperlipidemic effects of (L.) millsp. root in methylglyoxal-induced diabetic rats.

Chin J Physiol 2022 May-Jun;65(3):125-135

Department of Food Science and Biotechnology, Da-Yeh University, Changhua, Taiwan.

Cajanus cajan (L.) Millsp., known as pigeon pea, is one of the major grain legume crops of the tropical world. Read More

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Activity of Some Medicinal Plants on Blood Coagulation.

Turk J Pharm Sci 2022 Jun;19(3):330-335

An-Najah National University, Faculty of Science, Department of Statistics, Nablus, Palestine.

Objectives: The concern for finding natural and curative agents without adverse side effects has prompted the interest in discovering hemostatic agents from plants. Therefore, activity of L. (Aizoaceae), Boiss. Read More

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Long-read genotyping with SLANG (Simple Long-read loci Assembly of Nanopore data for Genotyping).

Appl Plant Sci 2022 May-Jun;10(3):e11484. Epub 2022 Jun 14.

Institute of Plant Sciences, University of Regensburg, Universitätsstraße 31 Regensburg BY D-93053 Germany.

Premise: Most phylogenomic library preparation methods and bioinformatic analysis tools in restriction site-associated DNA sequencing (RADseq)/genotyping-by-sequencing (GBS) studies are designed for use with Illumina data. The lack of alternative bioinformatic pipelines hinders the exploration of long-read multi-locus data from other sequencing platforms. The Simple Long-read loci Assembly of Nanopore data for Genotyping (SLANG) pipeline enables locus assembly, orthology estimation, and single-nucleotide polymorphism (SNP) calling using Nanopore-sequenced multi-locus data. Read More

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Ancient DNA extraction methods for herbarium specimens: When is it worth the effort?

Appl Plant Sci 2022 May-Jun;10(3):e11477. Epub 2022 Jun 15.

Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium) University of Göttingen, Untere Karspüle 2 37073 Göttingen Germany.

Premise: Herbaria harbor a tremendous number of plant specimens that are rarely used for molecular systematic studies, largely due to the difficulty in extracting sufficient amounts of high-quality DNA from the preserved plant material.

Methods: We compared the standard Qiagen DNeasy Plant Mini Kit and a specific protocol for extracting ancient DNA (aDNA) (the -phenacylthiazolium bromide and dithiothreitol [PTB-DTT] extraction method) from two different plant genera ( and ). The included herbarium materials covered about two centuries of plant collections. Read More

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Transcriptomic Profile Analysis of Response to Flower Extracts.

Evid Based Complement Alternat Med 2022 21;2022:7767940. Epub 2022 Jun 21.

Department of Basic Science and Oral Biology, Faculty of Dentistry, Universiti Sains Islam Malaysia, Kuala Lumpur 55100, Malaysia.

Background: has been used as a traditional medicine to treat oral health diseases such as dental caries and periodontitis. is a common bacterium that initiates dental caries at an early stage.

Aim: The aim of this study was to determine the mode of action of . Read More

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Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform.

Synth Biol (Oxf) 2022 21;7(1):ysac008. Epub 2022 May 21.

Synthetic Biology & Bioenergy, J. Craig Venter Institute, La Jolla, CA, USA.

Cell-free expression (CFE) systems are one of the main platforms for building synthetic cells. A major drawback is the orthogonality of cell-free systems across species. To generate a CFE system compatible with recently established minimal cell constructs, we attempted to optimize a bacterium-based CFE system using lysates of the genome-minimized cell JCVI-syn3A (Syn3A) and its close phylogenetic relative (Mcap). Read More

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Preparation and characterization analysis of biofuel derived through seed extracts of Ricinus communis (castor oil plant).

Sci Rep 2022 Jun 30;12(1):11021. Epub 2022 Jun 30.

Centre for Excellence-Indigenous Knowledge, Innovative Technology Transfer and Entrepreneurship, Dambi Dollo University, Dembi Dolo, Ethiopia.

The current study assesses the prospect of using R. Communis seed oil as a substitute fuel for diesel engines. Biodiesel is prepared from the R. Read More

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Comparison between MACSprep™ forensic sperm microbead kit and Erase Sperm Isolation kit for the enrichment of sperm fractions recovered from sexual assault samples.

Int J Legal Med 2022 Jun 30. Epub 2022 Jun 30.

Forensic Genetics Unit, University Center of Legal Medicine, Lausanne - Geneva, Lausanne University Hospital and University of Lausanne, Chemin de la Vulliette 4, 1000, Lausanne 25, Switzerland.

Sexual assault samples often contain mixtures of cells coming from at least two donors. Ideally, one would need to separate the cells into two cellular fractions: one consisting of the alleged aggressor's spermatozoa (the sperm fraction) and the other containing the victim's epithelial cells (the non-sperm fraction). This separation increases the probability of obtaining the alleged offender's autosomal DNA profile. Read More

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Coffee and cigarettes: Modulation of high and low sensitivity α4β2 nicotinic acetylcholine receptors by n-MP, a biomarker of coffee consumption.

Neuropharmacology 2022 Jun 27;216:109173. Epub 2022 Jun 27.

Department of Pharmacology and Therapeutics, University of Florida, PO Box 100267, Gainesville, FL, 32610-0267, (RLP, MK, CS), USA.

Smokers report particular appreciation for coffee with their first cigarettes of the day. We investigated with voltage-clamp experiments, effects of aqueous extracts (coffees) of unroasted and roasted coffee beans on the activity of human brain nicotinic acetylcholine receptor (nAChR) subtypes expressed in Xenopus oocytes, looking at complex brews, low molecular weight (LMW) fractions, and specific compounds present in coffee. When co-applied with PNU-120596, a positive allosteric modulator (PAM), the coffees stimulated currents from cells expressing α7 nAChR that were larger than ACh controls. Read More

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Machine learning for multi-parametric breast MRI: radiomics-based approaches for lesion classification.

Phys Med Biol 2022 Jun 30. Epub 2022 Jun 30.

Department of Pathology and Diagnostics, Medical Physics Unit, Azienda Ospedaliera Universitaria Integrata Verona Sede di Borgo Trento, Piazzale Stefani 1, Verona, 37124, ITALY.

In the artificial intelligence era, machine learning techniques have gained more and more importance in the advanced analysis of medical images in several fields of modern medicine. Radiomics extracts a huge number of medical imaging features revealing key components of tumor phenotype that can be linked to genomic pathways. The multi-dimensional nature of radiomics requires highly accurate and reliable machine-learning methods to create predictive models for classification or therapy response assessment. Read More

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Withaferin A, a polyfunctional pharmacophore that includes covalent engagement of IPO5, is an inhibitor of influenza A replication.

Bioorg Med Chem 2022 Jun 18;69:116883. Epub 2022 Jun 18.

Department of Organic Chemistry, Faculty of Science, NCCR Chemical Biology, University of Geneva, 1211 Geneva, Switzerland. Electronic address:

Withaferin A, a natural steroidal lactone found in the extracts of Withania somnifera, is used extensively in traditional medicine and part of an ancient remedy in ayurvedic medicine. Prior investigations into its mode of action have shown withaferin to be a polyfunctional pharmacophore with the covalent engagement of a multitude of therapeutic targets. Herein, we report that withaferin A is also a covalent inhibitor of IPO5, an importin that translocates cargos from the cytosol to the nucleus. Read More

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PScL-DDCFPred: an ensemble deep learning-based approach for characterizing multiclass subcellular localization of human proteins from bioimage data.

Bioinformatics 2022 Jun 30. Epub 2022 Jun 30.

School of Computer Science and Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei, Nanjing, 210094, China.

Motivation: Characterization of protein subcellular localization has become an important and long-standing task in bioinformatics and computational biology, which provides valuable information for elucidating various cellular functions of proteins and guiding drug design.

Results: Here, we develop a novel bioimage-based computational approach, termed PScL-DDCFPred, to accurately predict protein subcellular localizations in human tissues. PScL-DDCFPred first extracts multiview image features, including global and local features, as base or pure features; Next, it applies a new integrative feature selection method based on stepwise discriminant analysis and generalized discriminant analysis to identify the optimal feature sets from the extracted pure features; Finally, a classifier based on deep neural network (DNN) and deep-cascade forest (DCF) is established. Read More

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Methods to Detect Neutrophil Extracellular Traps in Asthma.

Methods Mol Biol 2022 ;2506:281-295

Laboratory of Immunophysiology, GIGA Institute, Liège University, Liège, Belgium.

Neutrophil extracellular traps (NETs) have the ability to regulate many aspects of asthma pathology. NETs can be detected either in bronchoalveolar lavage fluids (BALF) or in lung biopsies. Here, we describe methods to quantify NETs in BALF, namely the quantification of cell-free DNA, or of myeloperoxidase (MPO) or neutrophil elastase (NE) complexed with cell-free DNA. Read More

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January 2022

Sustainable eco-friendly extraction of yellow natural dye from haar singhar (Nyctanthes arbor-tritis) for bio coloration of cotton fabric.

Environ Sci Pollut Res Int 2022 Jun 30. Epub 2022 Jun 30.

Applied Chemistry Research Center, PCSIR Laboratories Complex, Feruzepur Road Lahore, Lahore, Pakistan.

 The revival of natural dyes in different walks of life is due to stringent environmental standards imposed by many associations. For current studies, flowers of haar singhar (Nyctanthes arbor-tritis) has been chosen for bio-dyeing of cotton fabric using microwave irradiation techniques. For this purpose, liquid extracts and fabrics were exposed to microwave for 5 min at various conditions. Read More

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Green biosynthesis of magnetic iron oxide nanoparticles using Mentha longifolia for imatinib mesylate delivery.

IET Nanobiotechnol 2022 Jun 30. Epub 2022 Jun 30.

Department of Mechanical Engineering, University of Birjand, Birjand, Iran.

In this work, the rapid, facile, and eco-friendly green process was introduced in the preparation of β-cyclodextrin/magnetic iron oxide nanoparticles by using the aqueous Mentha longifolia extracts of Mentha longifolia. The obtained nanoparticles were characterised by Fourier transform infrared spectroscopy, x-ray powder diffraction, field emission scanning electron microscope, and thermogravimetric analysis. Also, effective factors on the synthesis of magnetic nanocomposites including temperature, concentration of the Mentha longifolia extract, and concentration of FeSO solution were optimised by Taguchi design. Read More

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Pharmacological potential of seaweed-associated heterotrophic Firmicutes.

Lett Appl Microbiol 2022 Jun 30. Epub 2022 Jun 30.

Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No, 1603, Cochin, -682018, Kerala State, India.

Seaweed-associated bacterial symbionts are sources of potential pharmacological properties. The present study resulted in the culture-dependent isolation of bioactive heterotrophs belonging to the bacterial phylum Firmicutes, which were dominated more than 30% of the 127 cultivable isolates, among which 23 of them showed potential antimicrobial activities against a wide range of pathogens. The symbionts isolated from the seaweed Sargassum wightii showed significant bioactivity. Read More

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First report of barley virus G infecting winter barley (Hordeum vulgare L.) in France.

Plant Dis 2022 Jun 30. Epub 2022 Jun 30.

INRA Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, INRA - Université de Bordeaux, CS20032, Villenave d'Ornon , France, 33882 cedex;

As part of a cereals virome project high throughput sequencing (HTS)-based viral indexing was performed on plants with symptoms of barley yellow dwarf disease collected in June (2017-2020) in the main French cereals production areas. Total RNAs from 32 individual plants were purified (RNeasy Plant Mini Kit, Qiagen, Courtaboeuf, France) and Illumina sequenced (2x150 nt) following ribodepletion (Genewiz-Azenta, Leipzig, Germany). Following quality trimming, reads for each sample were de novo assembled (CLC Genomics Workbench 21, Qiagen) [1] and contigs annotated by BlastX analysis. Read More

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Research Advances in Lotus Leaf as Chinese Dietary Herbal Medicine.

Am J Chin Med 2022 Jun 30:1-23. Epub 2022 Jun 30.

Department of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, Hubei, P. R. China.

Lotus leaf (Heye), the dry foliage of Gaertn, has been valuable as a dietary herbal medicine for thousands of years. Phytochemical studies indicated that alkaloids and flavonoids are the main components of Heye. Polysaccharides, terpenes, and amino acids are also active ingredients. Read More

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The Leaves of : A Functional Tea with Preventive and Therapeutic Potential of Type 2 Diabetes.

Am J Chin Med 2022 Jun 30:1-27. Epub 2022 Jun 30.

School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, P. R. China.

Type 2 diabetes mellitus (T2DM) has become a universal and chronic global public health concern and causes multiple complex complications. In order to meet the rapidly growing demand for T2DM treatment, increased research has been focused on hypoglycemic drugs. (Batal. Read More

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Transcutaneous Electrical Nerve Stimulation of PC5 and PC6 Acupoints Increases Sympathovagal Balance but Not Oxidative Stress in Healthy Subjects: A Randomized Clinical Trial.

J Acupunct Meridian Stud 2021 Oct;14(5):183-192

Department of Physiological Sciences, Biomedical Institute, Federal University of the State of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

Background: Transcutaneous electrical acupoint stimulation (TEAS) improves autonomic balance and reduces oxidative stress in subjects with chronic diseases, that decreases the risk of low-grade chronic inflammatory diseases, including cardiovascular diseases. However, these beneficial effects have never been demonstrated in healthy subjects. Objectives: To evaluate the acute effects of TEAS on autonomic balance and oxidative stress of healthy subjects. Read More

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October 2021

The exploration of bio-inspired copper oxide nanoparticles: synthesis, characterization and in-vitro biological investigations.

Heliyon 2022 Jun 11;8(6):e09726. Epub 2022 Jun 11.

Research Centre for Solar Energy, Department of Physics, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, 522502, Andhra Pradesh, India.

The paper describes the synthesis and characterization of copper oxide nanoparticles (CuO NPs) using the mixture of plant rhizome extracts and as a reducing agent. The prepared CuO nanoparticles are characterized and confirmed their formation based on data obtained from powder X-ray diffraction spectroscopy, Fourier Transmission Infrared, Ultraviolet-Visible spectra, Field Emission Scanning Electron Microscopy images, Energy Dispersive X-ray analysis and Dynamic light scattering techniques and data reveal that the average size of CuO Nps was 103.4 nm. Read More

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Isolation and Quantification of the Hepatoprotective Flavonoids From (Willd.) R. J. Wang With Bio-Enzymatic Method Against NAFLD by UPLC-MS/MS.

Front Pharmacol 2022 13;13:890148. Epub 2022 Jun 13.

School of Public Health, Shaanxi University of Chinese medicine, Xi'an, China.

Flavonoids were the major phytochemicals against hepatic peroxidative injury in (Willd.) R. J. Read More

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Characterization of the B-Cell Epitopes of Histones H4 and H2A Recognized by Sera From Patients With Liver Cysts.

Front Cell Infect Microbiol 2022 13;12:901994. Epub 2022 Jun 13.

Universidad Abierta Interamericana (UAI), Centro de Altos Estudios en Ciencias Humanas y de la Salud (CAECIHS), Buenos Aires, Argentina.

Cystic echinococcosis (CE) is a zoonotic disease worldwide distributed, caused by the cestode sensu lato (), with an incidence rate of 50/100,000 person/year and a high prevalence in humans of 5-10%. Serology has variable sensitivity and specificity and low predictive values. Antigens used are from the hydatid fluid and recombinant antigens have not demonstrated superiority over hydatid fluid. Read More

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