153,002 results match your criteria extracellular matrix

Peptide location fingerprinting identifies species- and tissue-conserved structural remodelling of proteins as a consequence of ageing and disease.

Matrix Biol 2022 May 23. Epub 2022 May 23.

Divisions of Cell Matrix Biology & Regenerative Medicine and Cardiovascular Sciences, School of Biological Science, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK. Electronic address:

Extracellular matrix (ECM) in the intervertebral disc (IVD), lung and artery are thought to undergo the age-dependant accumulation of damage by chronic exposure to mechanisms such as reactive oxygen species, proteases and glycation. It is unknown whether this damage accumulation is species-dependant (via differing lifespans and hence cumulative exposures) or whether it can influence the progression of age-related diseases such as atherosclerosis. Peptide location fingerprinting (PLF) is a new proteomic analysis method, capable of the non-targeted identification of structure-associated changes within proteins. Read More

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Implications of enigmatic transglutaminase 2 (TG2) in cardiac diseases and therapeutic developments.

Biochem Pharmacol 2022 May 23:115104. Epub 2022 May 23.

Department of Biological Sciences, Al al-bayt University, Al-Mafraq, Jordan.

Cardiac diseases are the leading cause of mortality and morbidity worldwide. Mounting evidence suggests that transglutaminases (TGs), tissue TG (TG2) in particular, are involved in numerous molecular responses underlying the pathogenesis of cardiac diseases. The TG family has several intra- and extracellular functions in the human body, including collagen cross-linking, angiogenesis, cell growth, differentiation, migration, adhesion as well as survival. Read More

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Bilayer silk fibroin/sodium alginate scaffold promotes vascularization and advances inflammation stage in full-thickness wound.

Biofabrication 2022 May 26. Epub 2022 May 26.

Biomedical Material and Engineering Research Center, Wuhan University of Technology, Wuhan 430070, Wuhan, Hubei, 430070, CHINA.

An ideal wound dressing for full-thickness wound regeneration should offer desirable biocompatibility, adequate mechanical properties, barrier function, and cellular regulation. Here, a bilayer scaffold resembling the hierarchical structure of human skin was developed using silk fibroin and sodium alginate. The upper membrane was prepared through casting and functioned as the epidermis, whereas the lower porous scaffold was prepared by freeze-drying and mimicked extracellular matrix structures. Read More

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Immunoresponsive microbiota-gut-on-chip reproduces barrier dysfunction, stromal reshaping and probiotics translocation under inflammation.

Biomaterials 2022 May 17;286:121573. Epub 2022 May 17.

Center for Advanced Biomaterials for [email protected], Istituto Italiano di Tecnologia, Naples, Italy. Electronic address:

Here, we propose an immune-responsive human Microbiota-Intestine axis on-chip as a platform able to reproduce the architecture and vertical topography of the microbiota with a complex extracellular microenvironment consisting of a responsive extra cellular matrix (ECM) and a plethora of immune-modulatory mediators released from different cell populations such as epithelial, stromal, blood and microbial species in homeostatic and inflamed conditions. Firstly, we developed a three-dimensional human intestine model (3D-hI), represented by an instructive and histologically competent ECM and a well-differentiated epithelium with mucus-covered microvilli. Then, we replicated the microenvironmental anaerobic condition of human intestinal lumen by fabricating a custom-made microbiota chamber (M) on the apical side of the Microbiota-human Intestine on chip (MihI-oC), establishing the physiological oxygen gradient occurring along the thickness of human small intestine from the serosal to the luminal side. Read More

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Down-Regulating Scar Formation by Microneedles Directly a Mechanical Communication Pathway.

ACS Nano 2022 May 26. Epub 2022 May 26.

Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.

Excessive extracellular matrix deposition drives fibroblasts into a state of high mechanical stress, exacerbating pathological fibrosis and hypertrophic scar formation, leading to tissue dysfunction. This study reports a minimally invasive and convenient approach to obtaining scarless tissue using a silk fibroin microneedle patch (SF MNs). We found that by tuning the MN size and density only, the biocompatible MNs significantly decreased the scar elevation index in the rabbit ear hypertrophic scar model and increased ultimate tensile strength close to regular skin. Read More

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Nanchangmycin regulates FYN, PTK2, and MAPK1/3 to control the fibrotic activity of human hepatic stellate cells.

Elife 2022 May 26;11. Epub 2022 May 26.

Division of Gastroenterology, Massachusetts General Hospital, Boston, United States.

Chronic liver injury causes fibrosis, characterized by the formation of scar tissue resulting from excessive accumulation of extracellular matrix (ECM) proteins. Hepatic stellate cell (HSC) myofibroblasts are the primary cell type responsible for liver fibrosis, yet there are currently no therapies directed at inhibiting the activity of HSC myofibroblasts. To search for potential anti-fibrotic compounds, we performed a high-throughput compound screen in primary human HSC myofibroblasts and identified 19 small molecules that induce HSC inactivation, including the polyether ionophore nanchangmycin (NCMC). Read More

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Microglia-mediated degradation of perineuronal nets promotes pain.

Science 2022 May 26:eabl6773. Epub 2022 May 26.

Department of Anesthesia, McGill University, Montreal, Quebec, Canada.

Activation of microglia in the spinal cord dorsal horn following peripheral nerve injury contributes to the development of pain hypersensitivity. How activated microglia selectively enhance the activity of spinal nociceptive circuits is not well understood. We discovered that following peripheral nerve injury, microglia degrade extracellular matrix structures, perineuronal nets (PNNs), in lamina I of the spinal cord dorsal horn. Read More

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AP-1 Subunit JUNB Promotes Invasive Phenotypes in Endometriosis.

Reprod Sci 2022 May 26. Epub 2022 May 26.

Department of Obstetrics, Gynecology and Reproductive Biology, College of Human Medicine, Michigan State University, Grand Rapids, MI, 49503, USA.

Endometriosis is a disease defined by the presence of abnormal endometrium at ectopic sites, causing pain and infertility in 10% of women. Mutations in the chromatin remodeling protein ARID1A (AT-rich interactive domain-containing protein 1A) have been identified in endometriosis, particularly in the more severe deep infiltrating endometriosis and ovarian endometrioma subtypes. ARID1A has been shown to regulate chromatin at binding sites of the Activator Protein 1 (AP-1) transcription factor, and AP-1 expression has been shown in multiple endometriosis models. Read More

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Galactose Enhances Chondrogenic Differentiation of ATDC5 and Cartilage Matrix Formation by Chondrocytes.

Front Mol Biosci 2022 9;9:850778. Epub 2022 May 9.

School of Materials Science and Engineering, South China University of Technology, Guangzhou, China.

Galactose, an important carbohydrate nutrient, is involved in several types of cellular metabolism, participating in physiological activities such as glycosaminoglycan (GAG) synthesis, glycosylation, and intercellular recognition. The regulatory effects of galactose on osteoarthritis have attracted increased attention. In this study, cell models of ATDC5 and chondrocytes were prepared and cultured with different concentrations of galactose to evaluate its capacity on chondrogenesis and cartilage matrix formation. Read More

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Osteocyte β1 integrin loss causes low bone mass and impairs bone mechanotransduction in mice.

J Orthop Translat 2022 May 17;34:60-72. Epub 2022 May 17.

Department of Biochemistry, School of Medicine, Southern University of Science and Technology, Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, Shenzhen Key Laboratory of Cell Microenvironment, Shenzhen, 518055, China.

Background: The key focal adhesion protein β1 integrin plays an essential role in early skeletal development. However, roles of β1 integrin expression in osteocytes during the regulation of bone homeostasis and mechanotransduction are incompletely understood.

Materials And Methods: To study the in vivo function of osteocyte β1 integrin in bone, we utilized the 10-kb to generate mice with β1 integrin deletion in this cell type. Read More

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Biomechanical Regulatory Factors and Therapeutic Targets in Keloid Fibrosis.

Front Pharmacol 2022 9;13:906212. Epub 2022 May 9.

National Innovation and Attracting Talents "111" Base, Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.

Keloids are fibroproliferative skin disorder caused by abnormal healing of injured or irritated skin and are characterized by excessive extracellular matrix (ECM) synthesis and deposition, which results in excessive collagen disorders and calcinosis, increasing the remodeling and stiffness of keloid matrix. The pathogenesis of keloid is very complex, and may include changes in cell function, genetics, inflammation, and other factors. In this review, we aim to discuss the role of biomechanical factors in keloid formation. Read More

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Strong Bioinspired Polymer Hydrogel with Tunable Stiffness and Toughness for Mimicking the Extracellular Matrix.

ACS Macro Lett 2016 Nov 18;5(11):1217-1221. Epub 2016 Oct 18.

Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.

Inspired by the delicate architecture of hyaline articular cartilage, we report on a biomimetic polymer hydrogel that incorporates strong intermolecular hydrogen bonding between urethane-urethane linkages as well as urethane-ester linkages. The resultant hydrogel, containing ≈75% water, can endure a compressive stress up to 56 MPa with a strain of 98%, and exhibit tunable compressive modulus (0.19-1. Read More

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November 2016

Comparative transcriptome profiles of human dental pulp stem cells from maxillary and mandibular teeth.

Sci Rep 2022 May 25;12(1):8860. Epub 2022 May 25.

Center of Excellence for Medical Genomics, Medical Genomics Cluster, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

The molecular control of tooth development is different between the maxilla and mandible, contributing to different tooth shapes and locations; however, whether this difference occurs in human permanent teeth is unknown. The aim of this study was to investigate and compare the transcriptome profiles of permanent maxillary and mandibular posterior teeth. Ten participants who had a pair of opposing premolars or molars extracted were recruited. Read More

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Signaling events evoked by domain III of envelop glycoprotein of tick-borne encephalitis virus and West Nile virus in human brain microvascular endothelial cells.

Sci Rep 2022 May 25;12(1):8863. Epub 2022 May 25.

Laboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenského 73, 04181, Kosice, Slovak Republic.

Tick-borne encephalitis virus and West Nile virus can cross the blood-brain barrier via hematogenous route. The attachment of a virion to the cells of a neurovascular unit, which is mediated by domain III of glycoprotein E, initiates a series of events that may aid viral entry. Thus, we sought to uncover the post-attachment biological events elicited in brain microvascular endothelial cells by domain III. Read More

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In vitro proliferation and long-term preservation of functional primary rat hepatocytes in cell fibers.

Sci Rep 2022 May 25;12(1):8813. Epub 2022 May 25.

Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-Ku, Tokyo, 153-8505, Japan.

Primary hepatocytes are essential cellular resources for drug screening and medical transplantation. While culture systems have already succeeded in reconstituting the biomimetic microenvironment of primary hepatocytes, acquiring additional capabilities to handle them easily as well as to expand them remains unmet needs. This paper describes a culture system for primary rat hepatocytes, based on cell fiber technology, that brings scalability and handleability. Read More

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Pathophysiology of Age-Related Macular Degeneration. Implications for Treatment.

Ophthalmic Res 2022 May 25. Epub 2022 May 25.

Age-related macular degeneration (AMD) is a complex, multifactorial, progressive retinal disease that affects millions of people worldwide and has become the leading cause of visual impairment in developed countries. The disease etiopathogenesis is not understood fully, although many triggers and processes that lead to dysfunction and degeneration of the retinal pigment epithelium (RPE) already have been identified. Thus, the lack of cellular control of oxidative stress, altered proteostasis, dysfunction of lipid homeostasis, and mitochondrial dysfunction form an internal feedback loop that causes the RPE to fail and allows accumulation of abnormal misfolded proteins and abnormal lipids that will form drusen. Read More

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Proteoglycans and Glycosaminoglycans in Central Nervous System Injury.

Am J Physiol Cell Physiol 2022 May 25. Epub 2022 May 25.

Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States.

The brain and spinal cord constitute the central nervous system (CNS), which when injured, can be exceedingly devastating. The mechanistic roles of proteoglycans (PGs) and their glycosaminoglycan (GAG) side chains in such injuries have been extensively studied. CNS injury immediately alters endothelial and extracellular matrix (ECM) PGs and GAGs. Read More

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Structural control of fibrin bioactivity by mechanical deformation.

Proc Natl Acad Sci U S A 2022 May 25;119(22):e2117675119. Epub 2022 May 25.

Department of Biomedical Engineering, University of Texas at Austin, Austin, TX 78712.

SignificanceFibrin plays a vital role in biology as the fibrous network that stabilizes blood clots and also through interaction with numerous blood components. While much is known about fibrin mechanics, comparatively little is known about how fibrin's mechanics influence its biochemistry. We show that structural changes in fibrin under mechanical tension reduces binding of tissue plasminogen activator, an enzyme that initiates lysis. Read More

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Rapid Profiling of Tumor-Immune Interaction Using Acoustically Assembled Patient-Derived Cell Clusters.

Adv Sci (Weinh) 2022 May 25:e2201478. Epub 2022 May 25.

Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, 47405, USA.

Tumor microenvironment crosstalk, in particular interactions between cancer cells, T cells, and myeloid-derived suppressor cells (MDSCs), mediates tumor initiation, progression, and response to treatment. However, current patient-derived models such as tumor organoids and 2D cultures lack some essential niche cell types (e.g. Read More

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The molecular mechanism research of cartilage calcification induced by osteoarthritis.

Bioengineered 2022 May;13(5):13082-13088

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

To explore the molecular mechanism of cartilage calcification induced by osteoarthritis (OA) based on distal-less homeobox gene 5 - alkaline phosphatase - integrin-binding sialoprotein - ecto-nucleotide pyrophosphatase 1 (DLX5-ALPL-IBSP-ENPP1) signal axis. Twenty-four rabbits were selected to build models of cartilage calcification induced by OA and randomly divided into 3 groups. The first group was the normal group whose rabbits were injected into 0. Read More

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Bioleaching of rare earths elements from phosphate rock using Acidothiobacillus ferrooxidans.

Lett Appl Microbiol 2022 May 25. Epub 2022 May 25.

College of Resource and Environmental Engineering, Guizhou University, Guizhou Karst Environmental Ecosystems Observation and Research Station, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guiyang, 550025, China.

Phosphate rock containing rare earth elements (REEs) is considered one of the most promising potential secondary sources of REEs, as evidenced by large tonnages of phosphate rock mined annually. The bioleaching of REEs from phosphate rock using A. ferrooxidans was done for the first time in this study, and it was found to be greater than abiotic leaching and was more environmentally friendly. Read More

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Elasticity-associated rebinding rate of molecular bonds between soft elastic media.

Biophys J 2022 May 23. Epub 2022 May 23.

Key Laboratory of Mechanics on Disaster and Environment in Western China, Ministry of Education, College of Civil Engineering and Mechanics, Lanzhou University, Lanzhou, Gansu 730000, China. Electronic address:

A quantitative understanding of how cells interact with their extracellular matrix via molecular bonds is fundamental for many important processes in cell biology and engineering. In these interactions, the deformability of cells and matrix are usually comparable with that of the bonds, making their rebinding events globally coupled with the deformation states of whole systems. Unfortunately, this important principle is not realized or adopted in most conventional theoretical models for analyzing cellular adhesions. Read More

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CircSLC8A1 targets miR-181a-5p/HIF1AN pathway to inhibit the growth, migration and extracellular matrix deposition of human keloid fibroblasts.

Burns 2022 Apr 22. Epub 2022 Apr 22.

Department of Pediatric Burn and Plastic Surgery, Fujian Children's Hospital, Fuzhou, Fujian 350000, China.

Background: Circular RNAs (circRNAs) are identified as important regulators in human diseases, including keloid. The purpose of this study is to reveal the role and molecular mechanism of circSLC8A1 in keloid formation.

Methods: Expression of circSLC8A1, microRNA (miR)-181a-5p, and hypoxia inducible factor 1 alpha inhibitor (HIF1AN) were detected by quantitative real-time PCR. Read More

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Epididymal white adipose tissue promotes angiotensin II-induced cardiac fibrosis in an exosome-dependent manner.

Transl Res 2022 May 21. Epub 2022 May 21.

Department of Cardiology, the First Affiliated Hospital, Harbin Medical University, Harbin, 150001, China; NHC Key Laboratory of Cell Translation, Harbin Medical University, Heilongjiang, 150001, China; Key Laboratory of Cardiac Diseases and Heart Failure, Harbin Medical University, Harbin, 150001, China; Key Laboratory of Hepatosplenic Surgery, Harbin Medical University, Ministry of Education, Harbin, 150001, China. Electronic address:

Cardiac fibrosis is a process characterized by extracellular matrix accumulation leading to myocardial dysfunction. Angiotensin II (Ang II) has been shown to play an important role in the pathogenesis of cardiac fibrosis. However, the underlying mechanisms are not well established. Read More

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Granular Matrigel: Restructuring a Trusted Extracellular Matrix Material for Improved Permeability.

Biomed Mater 2022 May 24. Epub 2022 May 24.

Department of Chemical and Biological Engineering, Center for Biofilm Engineering, Montana State University Bozeman, PO BOX 172520, Bozeman, Montana, 59717-2520, UNITED STATES.

Matrigel is a polymeric extracellular matrix (ECM) material produced by mouse cancer cells. Over the past four decades, Matrigel has been shown to support a wide variety of two- and three-dimensional cell and tissue culture applications including organoids. Despite its widespread use, transport of molecules, cells, and colloidal particles through Matrigel can be limited. Read More

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Encapsulation of Cells in a Collagen Matrix Surrounded by an Alginate Hydrogel Shell for 3D Cell Culture.

ACS Biomater Sci Eng 2022 May 24. Epub 2022 May 24.

Laboratoire Colloïdes et Matériaux Divisés, CBI, ESPCI Paris, Université PSL, CNRS, 10 rue Vauquelin, F-75005 Paris, France.

Numerous techniques for mammalian cell culture have been developed to mimic the complex in vivo three-dimensional structure of tissues and organs. Among them, the sole use of proteins to create a matrix where cells are embedded already gives rise to self-organized multicellular assemblies. Loading cells in a controlled extracellular matrix along with cell culture and monitoring through a strategy that is compatible with pipetting tools would be beneficial for high throughput screening applications or simply for a standardized method. Read More

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Multifaceted control of E-cadherin dynamics by the Adaptor Protein Complex 1 during epithelial morphogenesis.

Mol Biol Cell 2022 May 24:mbcE21120598. Epub 2022 May 24.

School of Biosciences and Bateson Centre, The University of Sheffield, Sheffield S10 2TN, UK.

Intracellular trafficking regulates the distribution of transmembrane proteins including the key determinants of epithelial polarity and adhesion. The Adaptor Protein 1 (AP-1) complex is the key regulator of vesicle sorting, which binds many specific cargos. We examined roles of the AP-1 complex in epithelial morphogenesis, using the wing as a paradigm. Read More

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The lncRNA PILA promotes NF-κB signaling in osteoarthritis by stimulating the activity of the protein arginine methyltransferase PRMT1.

Sci Signal 2022 May 24;15(735):eabm6265. Epub 2022 May 24.

Clinical Research Centre, Zhujiang Hospital, Southern Medical University, 510280 Guangzhou, Guangdong, China.

Inflammatory cytokine-induced activation of nuclear factor κB (NF-κB) signaling plays a critical role in the pathogenesis of osteoarthritis (OA). We identified PILA as a long noncoding RNA (lncRNA) that enhances NF-κB signaling and OA. The abundance of PILA was increased in damaged cartilage from patients with OA and in human articular chondrocytes stimulated with the proinflammatory cytokine tumor necrosis factor (TNF). Read More

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EMMPRIN Promotes the Expression of MMP-9 and Exacerbates Neurological Dysfunction in a Mouse Model of Intracerebral Hemorrhage.

Neurochem Res 2022 May 24. Epub 2022 May 24.

Departments of Cerebrovascular Diseases and Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou, 450001, Henan, China.

Extracellular matrix metalloproteinase inducer (EMMPRIN) has been shown to be a vital inflammatory mediator in several neurological and neurodegenerative diseases. However, the role of EMMPRIN in intracerebral hemorrhage (ICH) remains unexplored. In this study, we aimed to exploit a highly selective monoclonal anti-EMMPRIN antibody to functionally inhibit EMMPRIN activity and thus that of MMPs as the downstream effector. Read More

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[Integrins in cardiac fibrosis].

Med Sci (Paris) 2022 May 24;38(5):438-444. Epub 2022 May 24.

Paris Centre de recherche cardiovasculaire (PARCC), Inserm U.970, 56 rue Leblanc, 75015 Paris, France.

For the last 20 years, integrins have been a therapeutic target of interest in the treatment of fibrotic diseases, particularly regarding the integrins of the αV family. Initially developed as anti-cancer drugs but with modest benefits, inhibitors of integrins (such as the anti-αV cilengitide) have shown interesting anti-fibrotic effects in different organs including the heart. Cardiac fibrosis is defined as an accumulation of stiff extracellular matrix in the myocardium, and ultimately leads to heart failure, one of the leading causes of mortality worldwide. Read More

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