7,757 results match your criteria expression peaked


It's about time; divergent circadian clocks in livers of mice and naked mole-rats.

FASEB J 2021 May;35(5):e21590

BGES Department, Center for Gene Regulation in Health and Disease, Cleveland State University, Cleveland, OH, USA.

Light is the key regulator of circadian clock, the time-keeping system synchronizing organism physiology and behavior with environmental day and night conditions. In its natural habitat, the strictly subterranean naked mole-rat (Heterocephalus glaber) has lived in a light-free environment for millennia. We questioned if this species retains a circadian clock and if the patterns of this clock and concomitant rhythms differed in liver tissue from mice and naked mole-rats. Read More

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Rearing temperature conditions (constant vs. thermocycle) affect daily rhythms of thermal tolerance and sensing in zebrafish.

J Therm Biol 2021 Apr 18;97:102880. Epub 2021 Feb 18.

Department of Physiology, Faculty of Biology, University of Murcia, 30100, Murcia, Spain. Electronic address:

In the wild, the environment does not remain constant, but periodically oscillates so that temperature rises in the daytime and drops at night, which generates a daily thermocycle. The effects of thermocycles on thermal tolerance have been previously described in fish. However, the impact of thermocycles on daytime-dependent thermal responses and daily rhythms of temperature tolerance and sensing expression mechanisms remain poorly understood. Read More

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Differential expression of groEL-1, incB, pyk-F, tal, hctA and omcB genes during Chlamydia trachomatis developmental cycle.

PLoS One 2021 15;16(4):e0249358. Epub 2021 Apr 15.

Centre for the AIDS Programme of Research in South Africa (CAPRISA), Durban, South Africa.

Chlamydia trachomatis infects squamous and columnar epithelia at the mucosal surface. Research on gene expression patterns of C. trachomatis has predominantly focused on non-native host cells, with limited data on growth kinetics and gene expression of chlamydia in keratinocytes. Read More

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Proteomic analysis of metabolic mechanisms associated with fatty acid biosynthesis during Styrax tonkinensis kernel development.

J Sci Food Agric 2021 Apr 15. Epub 2021 Apr 15.

Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancouver, British Columbia, V6T 1Z4, Canada.

Background: Styrax tonkinensis is a white-flowered tree with considerable potential as a feedstock source for biodiesel production from the oily seed contained within its nutlike drupes. Transcriptome changes during oil accumulation have been previously reported, but not concurrent changes in the proteome.

Results: Using proteomic analysis of samples collected at 50, 70, 100 and 130 days after flowering (DAF), we identified 1472 differentially expressed proteins (DEPs). Read More

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Mechanism of scar formation following Roux-en-Y choledochojejunostomy in a novel rat model of obstructive jaundice.

Ann Transl Med 2021 Mar;9(6):456

Department of Hepatobiliary and Pancreaticosplenic Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Background: The present study aimed to analyze the scar formation mechanism following Roux-en-Y choledochojejunostomy (CJS) in a novel rat model of obstructive jaundice.

Methods: The biliary obstruction model of Sprague-Dawley (SD) rats was established in advance, and 24 rats were randomly divided into 4 groups (control group, 1-day ligation group, 3-day ligation group, and 5-day ligation group). Changes in postoperative weight, common bile duct diameter, and laboratory indexes were analyzed to determine the best operation time. Read More

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Roflumilast as a Potential Therapeutic Agent for Cecal Ligation and Puncture-Induced Septic Lung Injury.

J Invest Surg 2021 Apr 11:1-9. Epub 2021 Apr 11.

Department of Pharmacology, Ataturk University, Erzurum, Turkey.

Purpose/aims: This study focused on delineating the possible effects of roflumilast (ROF), a selective phosphodiesterase 4 (PDE4) inhibitor, in rats with cecal ligation and puncture (CLP)-induced polymicrobial sepsis, and investigated whether ROF can act as a protective agent in sepsis-induced lung damage.

Material And Methods: Four experimental groups were organized, each comprising eight rats: Control, Sepsis, Sepsis + ROF 0.5 mgkg, and Sepsis + ROF 1 mgkg groups. Read More

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Inflammatory profile in a canine model of hypothermic circulatory arrest.

J Surg Res 2021 Apr 8;264:260-273. Epub 2021 Apr 8.

Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, Maryland; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Background: Hypothermic circulatory arrest (HCA) is a technique used for complex repair of the aorta, but it can be associated with neurologic morbidity. To better understand the molecular changes that underlie ischemic brain injury, we assessed gene expression and cytokine/chemokine polypeptide concentration in brain tissue and cerebrospinal fluid (CSF) of canines that underwent two hours of HCA.

Materials And Methods: Adult male canines were cannulated peripherally for cardiopulmonary bypass, cooled to 18°C, and arrested for two hours. Read More

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Mannosylated gelatin nanoparticles enhanced inactivated PRRSV targeting dendritic cells and increased T cell immunity.

Vet Immunol Immunopathol 2021 May 5;235:110237. Epub 2021 Apr 5.

College of Life Science and Technology, Dalian University, Dalian, 116622, PR China; Institute of Immunology, Dalian University, Dalian, 116622, PR China. Electronic address:

The objective of the present work was to evaluate the efficacy of a novel antigen carrier using mannosylated gelatin nanoparticles with entrapped inactivated porcine reproductive and respiratory syndrome virus (PRRSV) in inducing T cell mediated immunity in vitro. Gelatin nanoparticles (GNP) were modified with mannose to form mannosylated gelatin nanoparticles (MnGNP), which can efficiently and specifically target monocyte derived dendritic cells (MoDCs). The inactivated PRRSV was encapsulated in the MnGNP and GNP, referred to as MnGNP-PRRSV and GNP-PRRSV, respectively. Read More

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Expression of FOXO transcription factors in the brain following traumatic brain injury.

Neurosci Lett 2021 Apr 7;753:135882. Epub 2021 Apr 7.

Graduate School of Wannan Medical College, Wuhu, 241002, Anhui, China; Department of Pathology, School of Basic Medical Sciences, Wannan Medical College, Wuhu, 241002, Anhui, China. Electronic address:

Traumatic brain injury (TBI) is a substantial clinical and social problem worldwide, causing high morbidity and mortality along with significant economic and medical costs. Forkhead box O transcription factors (FOXOs) have been found to play a critical role in the regulation of cell functions, such as nutrient metabolism, programmed cell death, and tumor suppression. In the central nervous system, FOXOs are reported to be pivotal regulators of learning and memory, neurite outgrowth, and axonal degeneration. Read More

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OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats.

Mol Med 2021 Apr 9;27(1):37. Epub 2021 Apr 9.

Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Objective: Ovarian tumour domain deubiquitinase with linear linkage specificity (OTULIN) is a potent negative regulator of the nuclear factor-κB (NF-κB) signalling pathway, and it plays a strong neuroprotective role following acute ischemic stroke. Electroacupuncture (EA) is an effective adjuvant treatment for reducing brain injury and neuroinflammation via the inhibition of NF-κB p65 nuclear translocation, but the underlying mechanism is not clear. The present study investigated whether OTULIN was necessary for EA to mitigate brain injury and glial cell activation in a transient middle cerebral artery occlusion (tMCAO) model in rats. Read More

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Gonadal somatic cell-specific transforming growth factor-β superfamily member in the Yesso scallop reveals gonadal somatic cell distribution during the reproductive phase.

Gene 2021 Apr 6;787:145627. Epub 2021 Apr 6.

Laboratory of Aquacultural Biology, Graduate School of Agricultural Science, Tohoku University, 468-1 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8572, Japan.

The objective of this study was to identify the gonadal somatic cells in the Yesso scallop using a novel molecular marker. This study is the first to identify the bone morphogenetic protein 2a (Bmp2a) gene as a gonadal somatic cell-specific gene in this bivalve. We performed a transcriptomic survey to identify the transforming growth factor-β (TGFβ) superfamily members that act in Yesso scallop gonad development. Read More

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Influenza infection impairs the gut's barrier properties and favors secondary enteric bacterial infection through reduced production of short-chain fatty acids.

Infect Immun 2021 Apr 5. Epub 2021 Apr 5.

Univ. Lille, US 41 - UMS 2014 - PLBS, U1019 - UMR 9017 - CIIL - Centre d'Infection et d'Immunité de Lille, F-59000 Lille, France

Along with respiratory tract disease , viral respiratory infections can also cause extrapulmonary complications with a potentially critical impact on health. In the present study, we used an experimental model of influenza A virus (IAV) infection to investigate the nature and outcome of the associated gut disorders. In IAV-infected mice, the signs of intestinal injury and inflammation, altered gene expression, and compromised intestinal barrier functions peaked on day 7 post-infection. Read More

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Consumption of miRNA-Mediated Insect-Resistant Transgenic Rice Pollen Does Not Harm Adults.

J Agric Food Chem 2021 Apr 3;69(14):4234-4242. Epub 2021 Apr 3.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

RNAi-based insect-resistant genetically engineered (IRGE) crops represent a promising approach for pest management by suppressing gene expressions or translation. A developed microRNA-mediated IRGE rice line expressing endogenous Csu-novel-260 shows significant resistance to target pests. The nontarget insect is an important pollinator used as a surrogate species for the ecological risk assessment of IRGE plants. Read More

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Characterization of paramyosin protein structure and gene expression during myogenesis in Pacific oyster (Crassostrea gigas).

Comp Biochem Physiol B Biochem Mol Biol 2021 Apr 1;255:110594. Epub 2021 Apr 1.

Institute of Marine and Environmental Technology, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, United States.

Paramyosin is a key component of thick filaments in invertebrate muscles. In this study, we isolated the full length cDNA of paramyosin from Pacific oyster (Crassostrea gigas), and determined its pattern of expression during myogenesis. The full length paramyosin (CgPM) cDNA contains an open reading frame (ORF) of 2586 bp encoding a 861-amino acid protein. Read More

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Transcriptome Analyses Throughout Chili Pepper Fruit Development Reveal Novel Insights into the Domestication Process.

Plants (Basel) 2021 Mar 19;10(3). Epub 2021 Mar 19.

Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Irapuato, Guanajuato 36824, Mexico.

Chili pepper ( spp.) is an important crop, as well as a model for fruit development studies and domestication. Here, we performed a time-course experiment to estimate standardized gene expression profiles with respect to fruit development for six domesticated and four wild chili pepper ancestors. Read More

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Characterization of two group III potato CDPKs, StCDPK22 and StCDPK24, that contain three EF-Hand motifs in their CLDs.

Plant Physiol Biochem 2021 May 9;162:716-729. Epub 2021 Mar 9.

Laboratorio de Transducción de Señales en Plantas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires (C.A.B.A.), Argentina; Departamento de Química Biológica, UBA, C.A.B.A, Argentina. Electronic address:

Four members of the potato (Solanum tuberosum L.) calcium-dependent protein kinase (CDPK) family StCDPK22/23/24 and StCDPK27, present three functional EF-hands motifs in their calmodulin-like domain (CLD). StCDPK22/23/24 are clustered in clade III-b1 with tomato and Arabidopsis CDPKs that lack the first EF-hand motif, while StCDPK27 is clustered in clade III-b3 with CDPKs that lack EF-hand 2. Read More

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Ethylene response factors 15 and 16 trigger jasmonate biosynthesis in tomato during herbivore resistance.

Plant Physiol 2021 Apr;185(3):1182-1197

Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, PR China.

Jasmonates (JAs) are phytohormones with crucial roles in plant defense. Plants accumulate JAs in response to wounding or herbivore attack, but how JA biosynthesis is triggered remains poorly understood. Here we show that herbivory by cotton bollworm (Helicoverpa armigera) induced both ethylene (ET) and JA production in tomato (Solanum lycopersicum) leaves. Read More

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Adiponectin regulates osteocytic MLO-Y4 cell apoptosis in a high-glucose environment through the AMPK/FoxO3a signaling pathway.

J Cell Physiol 2021 Apr 1. Epub 2021 Apr 1.

Center of Stomatology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Clinical studies have shown that persistent hyperglycemia following oxidative stress is associated with the apoptosis of osteocytes in diabetics. Adiponectin (APN) can ameliorate oxidative stress, and its receptors have been identified in bone-forming cells. However, the relationship between APN and osteocyte apoptosis has not been fully elucidated. Read More

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Protective Effect of Mitochondrially Targeted Peptide Against Oxidant Injury of Cone Photoreceptors Through Preventing Necroptosis Pathway.

J Biomed Nanotechnol 2021 Feb;17(2):279-290

Department of Ophthalmology, The Second Affiliated Hospital ofXi'an Medical University, Xi'an 710038, Shanxi, PR China.

Retinopathy is an eye disease caused by the death of retinal cells in the macular area and the surrounding choroid. As the retinal rod cell dysfunction and death lead to the loss of night vision, the disease will lead to visual dysfunction and blindness as the disease progresses. Because of the irreversible nature of cell death, gene therapy has become a research hotspot in the field of retinopathy. Read More

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February 2021

Enzymatic and transcriptomic analysis reveals the essential role of carbohydrate metabolism in freesia () corm formation.

PeerJ 2021 19;9:e11078. Epub 2021 Mar 19.

Department of Landscape Architecture, Shanghai Jiao Tong University, Shanghai, China.

Starch and sucrose metabolism plays a crucial role in the formation and development of bulbs in bulbous plants. However, these mechanisms remain unclear and unexplored in the corms of . Herein, we investigated the dynamics of the major form of carbohydrates and related enzyme activities and profiled the transcriptome of freesia corms at four developmental stages with the aim to reveal the relation between the expression of genes involved in the metabolism of carbohydrates and the accumulation of carbohydrates in corm developmental stages for further exploring the mechanism on the starch and sucrose metabolism regulating the formation and development of corms in . Read More

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[Insulin-like growth factor 1 (IGF-1) promotes phagocytic activity of mouse BV-2 microglial cells via activating PI3K/AKT signaling pathway].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Mar;37(3):199-204

Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, China. *Corresponding authors, E-mail:

Objective To investigate the effect of insulin-like growth factor 1 (IGF-1) on the phagocytic activity of mouse BV-2 microglial cells. Methods Western blotting was performed to detect the protein levels of IGF-1 and IGF-1 receptor (IGF-1R) in the murine brain after the establishment of acute central nervous system inflammation models by intraperitoneal lipopolysaccharide (LPS) injection (10 mg/kg). The protein level of IGF-1R on BV-2 microglial cells that had been stimulated by 500 ng/mL LPS for 4, 12 and 24 hours was measured by Western blotting. Read More

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The expression of VEGF and VEGFR in endotoxin induced otitis media with effusion in rats.

Int J Pediatr Otorhinolaryngol 2021 Mar 5;144:110669. Epub 2021 Mar 5.

Department of Otolaryngology Head and Neck Surgery, Beiijng Anzhen Hospital Affiliated with Capital Medical University, 2 Anzhen Road, Chaoyang District, Beijing, 100029, China.

Objective: To investigate the expression and correlation of vascular endothelial growth factor (VEGF) and its receptor with hypoxia-inducible factor-1 α (HIF-1 α) in otitis media with effusion (OME).

Methods: A rat model of OME was induced by injection of lipopolysaccharide (LPS) into the middle ear. Hematoxylin and eosin (HE) staining was used to observe the pathomorphological changes of the tympanic cavity in the middle ear of rats. Read More

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Aquaporin-4 is a potential drug target for traumatic brain injury via aggravating the severity of brain edema.

Burns Trauma 2021 Jan 15;9:tkaa050. Epub 2021 Mar 15.

State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing 400042, China.

Background: Traumatic brain edema (TBE) is caused by a specific water channel mediated by membrane aquaporins. Aquaporin-4 (AQP4) plays an especially important role in this process, but the relationship between AQP4 and TBE remains unclear. The purpose of this study was to explore expression of AQP4 in the hippocampus after traumatic brain injury (TBI), as well as the effect of brain edema on skeletal protein and its function in hippocampal neurons. Read More

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January 2021

Dynamic study into autophagy and apoptosis during orthodontic tooth movement.

Exp Ther Med 2021 May 26;21(5):430. Epub 2021 Feb 26.

Oral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.

Orthodontic tooth movement (OTM) has been widely observed worldwide. The OTM process is involved in several biological activities and can result in temporary hypoxia. The dynamic changes of autophagy and apoptosis during OTM have not, to the best of our knowledge, been previously reported. Read More

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The chemotactic cytokines in the cerebrospinal fluid of patients with neuroborreliosis.

Cytokine 2021 Jun 18;142:155490. Epub 2021 Mar 18.

Department of the Infectious Diseases and Neuroinfections, Medical University in Białystok, ul. Żurawia 14, 15-540 Białystok, Poland.

Background: The outcome of neuroborreliosis (NB) is variable and may partially depend on host-related immune factors. In NB, the cerebrospinal fluid (CSF) contains a large population of T lymphocytes, but the mechanisms and consequences of their recruitment have not been fully elucidated. We have studied expression of T lymphocyte chemoattractant cytokines in association with CSF cytometric parameters and clinical data in NB patients. Read More

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Low birth weight female piglets show altered intestinal development, gene expression, and epigenetic changes at key developmental loci.

FASEB J 2021 Apr;35(4):e21522

Comparative Perinatal Development, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium.

Intestinal development is compromised in low birth weight (LBW) pigs, negatively impacting their growth, health, and resilience. We investigated the molecular mechanisms of the altered intestinal maturation observed in neonatal and juvenile LBW female piglets by comparing the changes in intestinal morphology, gene expression, and methylation in LBW versus normal birth weight (NBW) female piglets. A total of 16 LBW/NBW sibling pairs were sacrificed at 0 hours, 8 hours, 10 days, and 8 weeks of age. Read More

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Histology-based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers.

Int Endod J 2021 Mar 14. Epub 2021 Mar 14.

Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.

Aim: To profile molecular changes in lipopolysaccharide (LPS)-induced experimental pulpitis in a rat model and explore the feasibility of a molecular-based diagnostic strategy for pulpitis.

Methodology: Seventy-three maxillary incisors of Sprague-Dawley rats were used to establish pulpitis models with LPS. Inflammatory grading was performed in four equal sections of the pulp divided from the injured site to the root apex. Read More

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Evaluating the influence of progesterone concentration and time of exposure on in vitro endometrial decidualisation.

Mol Cell Endocrinol 2021 Mar 11;528:111242. Epub 2021 Mar 11.

Division of Child Health, Obstetrics and Gynaecology, School of Medicine, University of Nottingham, Nottingham, UK. Electronic address:

This study aimed to evaluate the influence of progesterone (concentration and time of exposure) on endometrial decidualisation using an in vitro model cell line: Human Endometrial Stromal Cells (HESCs). HESCs exposed to progesterone (1 and 10 μM) had higher percentages of decidualised cells and higher expression of the decidual marker (Insulin Like Growth Factor Binding Protein 1 (IGFBP1)) compared with those exposed to (0.1 μM). Read More

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Endothelin-1 Upregulates Activin Receptor-Like Kinase-1 Expression via G/RhoA/Sp-1/Rho Kinase Pathways in Human Pulmonary Arterial Endothelial Cells.

Front Cardiovasc Med 2021 23;8:648981. Epub 2021 Feb 23.

Department of Cardiovascular Medicine, Fukushima Medical University, Fukushima, Japan.

Pulmonary arterial hypertension (PAH) is characterized by pulmonary vasoconstriction and organic stenosis. It has been demonstrated that endothelin-1 (ET-1) induces pulmonary vasoconstriction through the activation of RhoA. In addition, a gene mutation of activin receptor-like kinase (ACVRL)-1 is recognized in PAH patients. Read More

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February 2021

Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats.

Oxid Med Cell Longev 2021 24;2021:8891373. Epub 2021 Feb 24.

Department of Critical Care Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China.

Background: Albumin has been regarded as a potent antioxidant with free radical scavenging activities. Oxidative stress and neuronal apoptosis are responsible for its highly damaging effects on brain injury after intracerebral hemorrhage (ICH). Here, the present study investigated the neuroprotective effect of albumin against early brain injury after ICH and the potential underlying mechanisms. Read More

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February 2021