12,234 results match your criteria expression master

Novel and known miRNAs in zebu (Tharparkar) and crossbred (Karan-Fries) cattle under heat stress.

Funct Integr Genomics 2021 May 11. Epub 2021 May 11.

Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.

MicroRNAs (miRNAs) are small single-stranded non-coding RNAs that act as the master regulator of animal growth and development. RNA-RNA interaction is an important mechanism of gene regulation during biotic and abiotic stress. Heat stress (HS) is one of the most important abiotic factors which affect the growth, milk yield and reproductive health of the dairy animals. Read More

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Quantitative Proteomics Reveals that Hsp90 Inhibition Dynamically Regulates Global Protein Synthesis in Leishmania mexicana.

mSystems 2021 May 11;6(3). Epub 2021 May 11.

Department of Biosciences, Durham University, Durham, United Kingdom.

Heat shock protein 90 (Hsp90) is a conserved molecular chaperone responsible for the folding and maturation of nascent proteins. Hsp90 is regarded as a master regulator of protein homeostasis in the cell, and its inhibition affects the functions of a large array of client proteins. The classical Hsp90 inhibitor tanespimycin has shown potent antileishmanial activity. Read More

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Tribbles Homolog 3 Mediates the Development and Progression of Diabetic Retinopathy.

Diabetes 2021 May 11. Epub 2021 May 11.

University of Alabama at Birmingham, Birmingham, AL, USA.

The current understanding of molecular pathogenesis of diabetic retinopathy does not provide a mechanistic link between early molecular changes and the subsequent progression of the disease. In this study, we found that human diabetic retinas overexpressed TRIB3 and investigated the role of TRIB3 in diabetic retinal pathobiology in mice. We discovered that TRIB3 controlled major molecular events in early diabetic retinas via HIF1α-mediated regulation of retinal glucose flux, reprograming cellular metabolism, and governing inflammatory gene expression. Read More

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XPO1 inhibition with selinexor synergizes with proteasome inhibition in neuroblastoma by targeting nuclear export of IkB.

Transl Oncol 2021 May 8;14(8):101114. Epub 2021 May 8.

Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue Ullmann 813 Bronx, NY 10461, United States; Department of Pediatrics, Albert Einstein College of Medicine, United States.

Across many cancer types in adults, upregulation of the nuclear-to-cytoplasmic transport protein Exportin-1 (XPO1) correlates with poor outcome and responsiveness to selinexor, an FDA-approved XPO1 inhibitor. Similar data are emerging in childhood cancers, for which selinexor is being evaluated in early phase clinical studies. Using proteomic profiling of primary tumor material from patients with high-risk neuroblastoma, as well as gene expression profiling from independent cohorts, we have demonstrated that XPO1 overexpression correlates with poor patient prognosis. Read More

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Insights into immunopathology and triggering of apoptosis in chronic cerebral toxoplasmosis using murine models.

Trop Biomed 2021 Jun;38(2):53-62

Medical Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

Background: toxoplasmosis is a cosmopolitan protozoan disease with a wide range of neuropathology. Recent studies identified its potential association with several mental disorders e.g. Read More

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Gene regulation in climacteric fruit ripening.

Javier Brumos

Curr Opin Plant Biol 2021 May 7;63:102042. Epub 2021 May 7.

Institute of Molecular and Cellular Biology of Plants, Consejo Superior de Investigaciones Científicas, Universitat Politècnica de València, 46022, Valencia, Spain. Electronic address:

Seed dispersion and consequent plant propagation depend on the success of fruit ripening. Thus, ripening is a highly regulated developmental process aiming to maximize fruit organoleptic traits to attract herbivores. During ripening, the developing fruit experiences dramatic modifications, including color change, flavor improvement, and loss of firmness that are remarkably coordinated. Read More

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Myeloid lncRNA LOUP Mediates Opposing Regulatory Effects of RUNX1 and RUNX1-ETO in t(8;21) AML.

Blood 2021 05 10. Epub 2021 May 10.

Harvard Medical School Initiative for RNA Medicine, Harvard Medical School, Boston, Massachusetts, United States.

The mechanism underlying cell type-specific gene induction conferred by ubiquitous transcription factors as well as disruptions caused by their chimeric derivatives in leukemia is not well understood. Here we investigate whether RNAs coordinate with transcription factors to drive myeloid gene transcription. In an integrated genome-wide approach surveying for gene loci exhibiting concurrent RNA- and DNA-interactions with the broadly expressed transcription factor RUNX1, we identified the long noncoding RNA LOUP. Read More

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Improvement in the anti-inflammatory profile with lifelong physical exercise is related to clock genes expression in effector-memory CD4+ T cells in master athletes.

Exerc Immunol Rev 2021 ;27:67-83

University of Coimbra, Research Center for Sport and Physical Activity, Faculty of Sports Science and Physical Education, Coimbra, Portugal.

Purpose: Ageing is associated with alterations in the immune system as well as with alterations of the circadian rhythm. Immune cells show rhythmicity in execution of their tasks. Chronic inflammation (inflammaging), which is observed in the elderly, is mitigated by lifelong exercise. Read More

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January 2021

Academic application of Good Cell Culture Practice for induced pluripotent stem cells.

ALTEX 2021 May 6. Epub 2021 May 6.

IUF-Leibniz Research Institute for Environmental Medicine, Duesseldorf, Germany.

Human induced pluripotent stem cells (hiPSC) are a promising tool for replacing animal-based experiments. To warrant data reproducibility, quality-controlled research material is recommended. While the need for global harmonization of quality standards for stem cell banking centers, commercial providers, pre-clinical and clinical use of the cells is well documented, up to date there are no recommendations available for quality control of hiPSC in an academic research environment. Read More

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GSK-3β S9A overexpression leads murine hippocampal neural precursors to acquire an astroglial phenotype in vivo.

Dev Neurobiol 2021 May 6. Epub 2021 May 6.

Department of Molecular Neuropathology, Centro de Biología Molecular "Severo Ochoa", CBMSO, CSIC-UAM, Madrid, Spain.

The addition of new neurons to the existing hippocampal circuitry persists in the adult dentate gyrus (DG). During this process, named adult hippocampal neurogenesis (AHN), adult hippocampal progenitor cells (AHPs) give rise to newborn dentate granule cells (DGCs). The acquisition of a neuronal lineage by AHPs is tightly regulated by numerous signaling molecules and transcription factors. Read More

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Targeting SLC1A5 and SLC3A2/SLC7A5 as a Potential Strategy to Strengthen Anti-Tumor Immunity in the Tumor Microenvironment.

Front Immunol 2021 19;12:624324. Epub 2021 Apr 19.

Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.

Cancer cells are metabolically vigorous and are superior in the uptake of nutrients and in the release of the tumor microenvironment (TME)-specific metabolites. They create an acidic, hypoxic, and nutrient-depleted TME that makes it difficult for the cytotoxic immune cells to adapt to the metabolically hostile environment. Since a robust metabolism in immune cells is required for optimal anti-tumor effector functions, the challenges caused by the TME result in severe defects in the invasion and destruction of the established tumors. Read More

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SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial-mesenchymal transition by β-catenin and Beclin1/autophagy signaling in colorectal cancer.

Cell Death Dis 2021 May 5;12(5):449. Epub 2021 May 5.

Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Sex-determining region Y-box2 (SOX2), a master regulator of embryonic and induced pluripotent stem cells, drives cancer stem cells (CSCs) properties, fuels tumor initiation, and contributes to tumor aggressiveness. Our previous study has demonstrated the oncogenic role of SOX2 in colorectal cancer (CRC). In this study, we sought to elucidate the underlying mechanisms. Read More

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Curcumin promotes cholesterol efflux by regulating ABCA1 expression through miR-125a-5p/SIRT6 axis in THP-1 macrophage to prevent atherosclerosis.

J Toxicol Sci 2021 ;46(5):209-222

Graduate School of Hunan University of Chinese Medicine, China.

Objective: To seek out the effect of curcumin on cholesterol efflux in THP-1 macrophages and clarify its specific mechanism.

Methods: THP-1 macrophages were cultured with curcumin at different concentrations, followed by detection of the toxicity of curcumin to cells utilizing CCK-8 assay. Following culturing with serum-free ox-LDL, THP-1 macrophages were transfected with mi-miR-125a-5p, or in-miR-125a-5p, or pcDNA3. Read More

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January 2021

Novel antiadipogenic effect of menadione in 3T3-L1 cells.

Chem Biol Interact 2021 May 1;343:109491. Epub 2021 May 1.

Instituto de Investigaciones Bioquímicas de Bahía Blanca, Universidad Nacional Del Sur (UNS)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bahía Blanca, Argentina. Departamento de Biología, Bioquímica y Farmacia, UNS, Bahía Blanca, Argentina. Electronic address:

Inhibition of adipocyte differentiation can be used as a strategy for preventing adipose tissue expansion and, consequently, for obesity management. Since reactive oxygen species (ROS) have emerged as key modulators of adipogenesis, the effect of menadione (a synthetic form of vitamin K known to induce the increase of intracellular ROS) on 3T3-L1 preadipocyte differentiation was studied. Menadione (15 μM) increased ROS and lipid peroxidation, generating mild oxidative stress without affecting cell viability. Read More

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The lncRNA and the transcription factor WRKY42 target common cell wall EXTENSIN encoding genes to trigger root hair cell elongation.

Plant Signal Behav 2021 May 4:1920191. Epub 2021 May 4.

Fundación Instituto Leloir and IIBBA-CONICET, Buenos Aires, CP, Argentina.

Plant long noncoding RNAs (lncRNAs) are key chromatin dynamics regulators, directing the transcriptional programs driving a wide variety of developmental outputs. Recently, we uncovered how the lncRNA () directly recognizes the locus encoding the root hair (RH) master regulator () modulating its transcriptional activation and leading to low temperature-induced RH elongation. We further demonstrated that interacts with the transcription factor WRKY42 in a novel ribonucleoprotein complex shaping epigenetic environment and integrating signals governing RH growth and development. Read More

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Postoperative abdominal sepsis induces selective and persistent changes in CTCF binding within the MHC-II region of human monocytes.

PLoS One 2021 3;16(5):e0250818. Epub 2021 May 3.

Department of Anesthesiology, Heidelberg University Hospital, Heidelberg, Baden-Württemberg, Germany.

Background: Postoperative abdominal infections belong to the most common triggers of sepsis and septic shock in intensive care units worldwide. While monocytes play a central role in mediating the initial host response to infections, sepsis-induced immune dysregulation is characterized by a defective antigen presentation to T-cells via loss of Major Histocompatibility Complex Class II DR (HLA-DR) surface expression. Here, we hypothesized a sepsis-induced differential occupancy of the CCCTC-Binding Factor (CTCF), an architectural protein and superordinate regulator of transcription, inside the Major Histocompatibility Complex Class II (MHC-II) region in patients with postoperative sepsis, contributing to an altered monocytic transcriptional response during critical illness. Read More

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Isopentenyltransferases as master regulators of crop performance: their function, manipulation, and genetic potential for stress adaptation and yield improvement.

Plant Biotechnol J 2021 May 2. Epub 2021 May 2.

Department of Biology, Trent University, Peterborough, ON, Canada.

Isopentenyltransferase (IPT) in plants regulates a rate-limiting step of cytokinin (CTK) biosynthesis. IPTs are recognized as key regulators of CTK homeostasis and phytohormone crosstalk in both biotic and abiotic stress responses. Recent research has revealed the regulatory function of IPTs in gene expression and metabolite profiles including source-sink modifications, energy metabolism, nutrient allocation and storage, stress defence and signalling pathways, protein synthesis and transport, and membrane transport. Read More

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GSTZ1 sensitizes hepatocellular carcinoma cells to sorafenib-induced ferroptosis via inhibition of NRF2/GPX4 axis.

Cell Death Dis 2021 Apr 30;12(5):426. Epub 2021 Apr 30.

Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.

Increasing evidence supports that ferroptosis plays an important role in tumor growth inhibition. Sorafenib, originally identified as an inhibitor of multiple oncogenic kinases, has been shown to induce ferroptosis in hepatocellular carcinoma (HCC). However, some hepatoma cell lines are less sensitive to sorafenib-induced ferroptotic cell death. Read More

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A Review of the State of HIV Nursing Science With Sexual Orientation, Gender Identity/Expression Peoples.

J Assoc Nurses AIDS Care 2021 May-Jun 01;32(3):225-252

J. Craig Phillips, PhD, LLM, RN, ACRN, FAAN, is a Professor of Nursing and Vice Dean of Governance and Secretary, Faculty of Health Sciences, University of Ottawa, Ottawa, Canada. Jufri Hidayat, MSN, RN, is an MSN Graduate, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan. Kristen D. Clark, MSN, RN, is a PhD Candidate, School of Nursing, University of California, San Francisco, San Francisco, California, USA. Julia Melisek, BScN, RN, is a Master of Nursing Graduate Student, Faculty of Health Sciences, University of Ottawa, Ottawa, Canada. Monique S. Balthazar, DNP, PhD, FNP-BC, AGACNP-BC, is a Postdoctoral Fellow, Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, Georgia, USA. Albert G. D. Beck is a Senior Policy Analyst, Métis National Council, Ottawa, Canada, and an Indigenous Fellow, Indigenous Fellowship Programme, United Nations Office of the High Commissioner for Human Rights, Geneva, Switzerland. Scott E. Moore, PhD, APRN, AGPCNP-BC, is an Assistant Professor, Frances Payne Bolton School of Nursing, Case Western Reserve University, Cleveland, Ohio, USA. Carol Dawson-Rose, PhD, RN, FAAN, is a Professor and Chair, Department of Community Health Systems, School of Nursing, University of California, San Francisco, San Francisco, California, USA.

Abstract: Throughout the HIV pandemic, nurses have contributed to or led approaches to understanding the effects of HIV disease at individual and societal levels. Nurses have advocated for socially just care for more than a century, and our efforts have created a foundation on which to further build the state of HIV nursing science with sexual orientation and gender identity/expression (SOGI) Peoples. Nurses have also participated in the development of approaches to manage HIV disease for and in collaboration with populations directly affected by the disease. Read More

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Comparative Phosphoproteomics of Classical Bordetellae Elucidates the Potential Role of Serine, Threonine and Tyrosine Phosphorylation in Biology and Virulence.

Front Cell Infect Microbiol 2021 13;11:660280. Epub 2021 Apr 13.

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia.

The genus is divided into two groups: classical and non-classical. , and are known as classical bordetellae, a group of important human pathogens causing whooping cough or whooping cough-like disease and hypothesized to have evolved from environmental non-classical bordetellae. infections have increased globally driving the need to better understand these pathogens for the development of new treatments and vaccines. Read More

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Generation of biologically active recombinant human OCT4 protein from .

3 Biotech 2021 May 8;11(5):207. Epub 2021 Apr 8.

Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039 Assam India.

Octamer-binding transcription factor 4 (OCT4) is vital for early embryonic development and is a master regulator of pluripotency in embryonic stem cells. Notably, OCT4 is a key reprogramming factor to derive induced pluripotent stem cells, which have tremendous prospects in regenerative medicine. In the current study, we report heterologous expression and purification of human OCT4 in to produce pure recombinant protein under native conditions. Read More

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Induction of during the Crosstalk between Antigen Presenting Like-Cells MHCIICD83 and Splenocytes CD4IgM in Rainbow Trout.

Biology (Basel) 2021 Apr 13;10(4). Epub 2021 Apr 13.

Grupo de Marcadores Inmunológicos en Organismos Acuáticos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, 2340000 Valparaíso, Chile.

In fish, the spleen is one of the major immune organs in the animal, and the splenocytes could play a key role in the activation and modulation of the immune response, both innate and adaptive. However, the crosstalk between different types of immune cells in the spleen has been poorly understood. In this work, an in vitro strategy is carried out to obtain and characterize mononuclear splenocytes from rainbow trout, using biomarkers associated with lymphocytes (CD4 and IgM) and antigen-presenting cells (CD83 and MHC II). Read More

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Exon-Intron Differential Analysis Reveals the Role of Competing Endogenous RNAs in Post-Transcriptional Regulation of Translation.

Noncoding RNA 2021 Apr 16;7(2). Epub 2021 Apr 16.

Institute of Oncology Research, Faculty of Biomedical Sciences, Universita`Svizzera Italiana, 6500 Bellinzona, Switzerland.

Stressful conditions induce the cell to save energy and activate a rescue program modulated by mammalian target of rapamycin (mTOR). Along with transcriptional and translational regulation, the cell relies also on post-transcriptional modulation to quickly adapt the translation of essential proteins. MicroRNAs play an important role in the regulation of protein translation, and their availability is tightly regulated by RNA competing mechanisms often mediated by long noncoding RNAs (lncRNAs). Read More

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Role of SHH in Patterning Human Pluripotent Cells towards Ventral Forebrain Fates.

Cells 2021 Apr 16;10(4). Epub 2021 Apr 16.

Child Study Center, Yale University, New Haven, CT 06520, USA.

The complexities of human neurodevelopment have historically been challenging to decipher but continue to be of great interest in the contexts of healthy neurobiology and disease. The classic animal models and monolayer in vitro systems have limited the types of questions scientists can strive to answer in addition to the technical ability to answer them. However, the tridimensional human stem cell-derived organoid system provides the unique opportunity to model human development and mimic the diverse cellular composition of human organs. Read More

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Betulinic Acid Affects the Energy-Related Proteomic Profiling in Pancreatic Ductal Adenocarcinoma Cells.

Molecules 2021 Apr 24;26(9). Epub 2021 Apr 24.

Graduate Institute of Metabolism and Obesity Sciences, Taipei Medical University, Taipei 11031, Taiwan.

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease with a 5-year survival rate of <8%. Therefore, finding new treatment strategies against PDAC cells is an imperative issue. Betulinic acid (BA), a plant-derived natural compound, has shown great potential to combat cancer owing to its versatile physiological functions. Read More

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Eps15 Homology Domain-Containing Protein 3 Hypermethylation as a Prognostic and Predictive Marker for Colorectal Cancer.

Biomedicines 2021 Apr 22;9(5). Epub 2021 Apr 22.

Ph.D. Program in the Clinical Drug Development of Herbal Medicine, Taipei Medical University, Taipei 11031, Taiwan.

Colorectal cancer (CRC) arises from chromosomal instability, resulting from aberrant hypermethylation in tumor suppressor genes. This study identified hypermethylated genes in CRC and investigated how they affect clinical outcomes. Methylation levels of specific genes were analyzed from The Cancer Genome Atlas dataset and 20 breast cancer, 16 esophageal cancer, 33 lung cancer, 15 uterine cancer, 504 CRC, and 9 colon polyp tissues and 102 CRC plasma samples from a Taiwanese cohort. Read More

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Genistein Activates Transcription Factor EB and Corrects Niemann-Pick C Phenotype.

Int J Mol Sci 2021 Apr 19;22(8). Epub 2021 Apr 19.

Departament of Gastroenterology, School of Medicine, Pontificia Universidad Católica de Chile, Santiago 3580000, Chile.

Niemann-Pick type C disease (NPCD) is a lysosomal storage disease (LSD) characterized by abnormal cholesterol accumulation in lysosomes, impaired autophagy flux, and lysosomal dysfunction. The activation of transcription factor EB (TFEB), a master lysosomal function regulator, reduces the accumulation of lysosomal substrates in LSDs where the degradative capacity of the cells is compromised. Genistein can pass the blood-brain barrier and activate TFEB. Read More

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Identification of Novel Biomarkers and Candidate Drug in Ovarian Cancer.

J Pers Med 2021 Apr 19;11(4). Epub 2021 Apr 19.

Department of Obstetrics and Gynaecology, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan.

This paper investigates the expression of the CREB1 gene in ovarian cancer (OV) by deeply excavating the gene information in the multiple databases and the mechanism thereof. In short, we found that the expression of the CREB1 gene in ovarian cancer tissue was significantly higher than that of normal ovarian tissue. Kaplan-Meier survival analysis showed that the overall survival was significantly shorter in patients with high expression of the CREB1 gene than those in patients with low expression of the CREB1 gene, and the prognosis of patients with low expression of the CREB1 gene was better. Read More

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The Unity of Redox and Structural Remodeling of Brown Adipose Tissue in Hypothyroidism.

Antioxidants (Basel) 2021 Apr 12;10(4). Epub 2021 Apr 12.

Center for Electron Microscopy, Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia.

Brown adipose tissue (BAT) is important for maintaining whole-body metabolic and energy homeostasis. However, the effects of hypothyroidism, one of the most common diseases worldwide, which increases the risk of several metabolic disorders, on BAT redox and metabolic homeostasis remain mostly unknown. We aimed to investigate the dynamics of protein expression, enzyme activity, and localization of antioxidant defense (AD) enzymes in rat interscapular BAT upon induction of hypothyroidism by antithyroid drug methimazole for 7, 15, and 21 days. Read More

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Physiological and Transcriptomic Analysis Provide Insight into Low Temperature Enhancing Hypericin Biosynthesis in .

Molecules 2021 Apr 15;26(8). Epub 2021 Apr 15.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.

Hypericin (Hyp), well-known as an antidepressant, is mainly extracted from . Although Hyp accumulation and biomass are greater at lower compared to higher temperature, the regulation mechanism has not been reported. Here, the physiological characteristics and transcriptome of grown at 15 and 22 °C were determined and analyzed by HPLC and de novo sequencing. Read More

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